Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62563.t1 | XP_004508161.1 | 96.7 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 1.40E-22 | 114.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62563.t1 | Q9M7X7 | 83.3 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 9.6e-20 | 96.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62563.t1 | I3SZB6 | 96.7 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 1.0e-22 | 114.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene62563 | 0.810098 | 1.36E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene62563 | 0.803027 | 4.24E-49 | -1.69E-46 |
| MS.gene050303 | MS.gene62563 | 0.813502 | 2.48E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene62563 | 0.814227 | 1.72E-51 | -1.69E-46 |
| MS.gene051330 | MS.gene62563 | 0.801332 | 9.47E-49 | -1.69E-46 |
| MS.gene05134 | MS.gene62563 | 0.838089 | 3.54E-57 | -1.69E-46 |
| MS.gene051700 | MS.gene62563 | 0.805954 | 1.04E-49 | -1.69E-46 |
| MS.gene05188 | MS.gene62563 | 0.814631 | 1.40E-51 | -1.69E-46 |
| MS.gene052133 | MS.gene62563 | 0.802226 | 6.20E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene62563 | 0.804237 | 2.38E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene62563 | 0.821699 | 3.51E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene62563 | 0.826328 | 2.88E-54 | -1.69E-46 |
| MS.gene05364 | MS.gene62563 | 0.801333 | 9.46E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene62563 | 0.843491 | 1.36E-58 | -1.69E-46 |
| MS.gene056091 | MS.gene62563 | 0.807632 | 4.60E-50 | -1.69E-46 |
| MS.gene056631 | MS.gene62563 | 0.837057 | 6.50E-57 | -1.69E-46 |
| MS.gene058216 | MS.gene62563 | 0.80168 | 8.03E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene62563 | 0.815767 | 7.81E-52 | -1.69E-46 |
| MS.gene058908 | MS.gene62563 | 0.820398 | 7.00E-53 | -1.69E-46 |
| MS.gene058911 | MS.gene62563 | 0.811025 | 8.60E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene62563 | 0.807446 | 5.03E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene62563 | 0.842393 | 2.66E-58 | -1.69E-46 |
| MS.gene060429 | MS.gene62563 | 0.910108 | 2.71E-82 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene62563 | MS.gene73093 | PPI |
| MS.gene043645 | MS.gene62563 | PPI |
| MS.gene62563 | MS.gene035825 | PPI |
| MS.gene035826 | MS.gene62563 | PPI |
| MS.gene064351 | MS.gene62563 | PPI |
| MS.gene99872 | MS.gene62563 | PPI |
| MS.gene043643 | MS.gene62563 | PPI |
| MS.gene62563 | MS.gene043595 | PPI |
| MS.gene035828 | MS.gene62563 | PPI |
| MS.gene043593 | MS.gene62563 | PPI |
| MS.gene064353 | MS.gene62563 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62563.t1 | MTR_4g121877 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 5.36e-37 | 117 |
| MS.gene62563.t1 | MTR_4g121870 | 93.333 | 60 | 4 | 0 | 1 | 60 | 137 | 196 | 8.56e-36 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62563.t1 | AT3G06680 | 83.333 | 60 | 10 | 0 | 1 | 60 | 23 | 82 | 1.68e-30 | 102 |
| MS.gene62563.t1 | AT3G06700 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 2.51e-30 | 100 |
| MS.gene62563.t1 | AT3G06700 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 2.51e-30 | 100 |
| MS.gene62563.t1 | AT3G06700 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 2.51e-30 | 100 |
| MS.gene62563.t1 | AT3G06680 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 4.39e-30 | 100 |
| MS.gene62563.t1 | AT3G06680 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 4.39e-30 | 100 |
Find 13 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTGTGACGATGCCTCTT+TGG | 0.332537 | 7.3:-69013079 | None:intergenic |
| CTCTGGTTCCTCAAGAACTT+TGG | 0.404366 | 7.3:-69013214 | None:intergenic |
| TCTTGTGATCTTTGTAAGAC+TGG | 0.406426 | 7.3:-69013042 | None:intergenic |
| TATGCTTCCTTGCATACCTC+TGG | 0.420261 | 7.3:-69013231 | None:intergenic |
| ATCGTCACACTTCAACCAAA+GGG | 0.488627 | 7.3:+69013089 | MS.gene62563:CDS |
| ATGATGAATACATACCCCTT+TGG | 0.493133 | 7.3:-69013104 | None:intergenic |
| TCTTACAAAGATCACAAGAA+TGG | 0.554926 | 7.3:+69013046 | MS.gene62563:CDS |
| CATCGTCACACTTCAACCAA+AGG | 0.595920 | 7.3:+69013088 | MS.gene62563:CDS |
| AGATGGATCCAAAGTTCTTG+AGG | 0.596761 | 7.3:+69013206 | MS.gene62563:intron |
| TGAGGAACCAGAGGTATGCA+AGG | 0.624057 | 7.3:+69013224 | MS.gene62563:CDS |
| CAAAGTTCTTGAGGAACCAG+AGG | 0.651910 | 7.3:+69013215 | MS.gene62563:CDS |
| TTTGTAAGACTGGTTGTGAG+CGG | 0.659865 | 7.3:-69013032 | None:intergenic |
| TCGTCACACTTCAACCAAAG+GGG | 0.708509 | 7.3:+69013090 | MS.gene62563:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCTTACAAAGATCACAAGAA+TGG | + | chr7.3:69013046-69013065 | MS.gene62563:CDS | 30.0% | |
| TTGTGATTGATTACTGTAGA+TGG | + | chr7.3:69013189-69013208 | MS.gene62563:intron | 30.0% | |
| ! | AGGAAGCATAACAAGAAAAA+TGG | + | chr7.3:69013244-69013263 | MS.gene62563:CDS | 30.0% |
| ATGATGAATACATACCCCTT+TGG | - | chr7.3:69013107-69013126 | None:intergenic | 35.0% | |
| TCTTGTGATCTTTGTAAGAC+TGG | - | chr7.3:69013045-69013064 | None:intergenic | 35.0% | |
| AGATGGATCCAAAGTTCTTG+AGG | + | chr7.3:69013206-69013225 | MS.gene62563:intron | 40.0% | |
| ATCGTCACACTTCAACCAAA+GGG | + | chr7.3:69013089-69013108 | MS.gene62563:CDS | 40.0% | |
| ATGGCATCAAAAAGCCAAAG+AGG | + | chr7.3:69013065-69013084 | MS.gene62563:CDS | 40.0% | |
| ! | TTTGTAAGACTGGTTGTGAG+CGG | - | chr7.3:69013035-69013054 | None:intergenic | 40.0% |
| CAAAGTTCTTGAGGAACCAG+AGG | + | chr7.3:69013215-69013234 | MS.gene62563:CDS | 45.0% | |
| CATCGTCACACTTCAACCAA+AGG | + | chr7.3:69013088-69013107 | MS.gene62563:CDS | 45.0% | |
| TATGCTTCCTTGCATACCTC+TGG | - | chr7.3:69013234-69013253 | None:intergenic | 45.0% | |
| TCGTCACACTTCAACCAAAG+GGG | + | chr7.3:69013090-69013109 | MS.gene62563:CDS | 45.0% | |
| ! | CTCTGGTTCCTCAAGAACTT+TGG | - | chr7.3:69013217-69013236 | None:intergenic | 45.0% |
| GAAGTGTGACGATGCCTCTT+TGG | - | chr7.3:69013082-69013101 | None:intergenic | 50.0% | |
| ! | TGAGGAACCAGAGGTATGCA+AGG | + | chr7.3:69013224-69013243 | MS.gene62563:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 69013010 | 69013288 | 69013010 | ID=MS.gene62563 |
| chr7.3 | mRNA | 69013010 | 69013288 | 69013010 | ID=MS.gene62563.t1;Parent=MS.gene62563 |
| chr7.3 | exon | 69013010 | 69013111 | 69013010 | ID=MS.gene62563.t1.exon1;Parent=MS.gene62563.t1 |
| chr7.3 | CDS | 69013010 | 69013111 | 69013010 | ID=cds.MS.gene62563.t1;Parent=MS.gene62563.t1 |
| chr7.3 | exon | 69013208 | 69013288 | 69013208 | ID=MS.gene62563.t1.exon2;Parent=MS.gene62563.t1 |
| chr7.3 | CDS | 69013208 | 69013288 | 69013208 | ID=cds.MS.gene62563.t1;Parent=MS.gene62563.t1 |
| Gene Sequence |
| Protein sequence |
