AlfalfaGEDB | MS.gene66939

M. sativa cultivar XinJiangDaYe / MS.gene66939

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02229.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66939.t1	XP_004502969.2	58.1	62	19	1	131	185	58	119	1.10E-09	73.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66939.t1	O65154	49.2	63	24	1	131	185	19	81	6.2e-09	62.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66939.t1	A0A1S2YDV6	58.1	62	19	1	131	185	21	82	8.1e-10	73.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene66939	0.826516	2.60E-54	-1.69E-46
MS.gene049159	MS.gene66939	0.84261	2.33E-58	-1.69E-46
MS.gene049254	MS.gene66939	0.813275	2.78E-51	-1.69E-46
MS.gene050559	MS.gene66939	0.812403	4.31E-51	-1.69E-46
MS.gene050561	MS.gene66939	0.821231	4.51E-53	-1.69E-46
MS.gene050562	MS.gene66939	0.812922	3.32E-51	-1.69E-46
MS.gene050844	MS.gene66939	0.82408	9.80E-54	-1.69E-46
MS.gene051247	MS.gene66939	0.80213	6.49E-49	-1.69E-46
MS.gene051279	MS.gene66939	0.814068	1.86E-51	-1.69E-46
MS.gene051710	MS.gene66939	0.801499	8.75E-49	-1.69E-46
MS.gene051744	MS.gene66939	0.84664	1.91E-59	-1.69E-46
MS.gene051800	MS.gene66939	0.805029	1.63E-49	-1.69E-46
MS.gene052516	MS.gene66939	0.819239	1.29E-52	-1.69E-46
MS.gene052519	MS.gene66939	0.812561	3.98E-51	-1.69E-46
MS.gene052582	MS.gene66939	0.801932	7.13E-49	-1.69E-46
MS.gene052773	MS.gene66939	0.804976	1.67E-49	-1.69E-46
MS.gene052863	MS.gene66939	0.818101	2.34E-52	-1.69E-46
MS.gene052896	MS.gene66939	0.811963	5.38E-51	-1.69E-46
MS.gene053226	MS.gene66939	0.807616	4.63E-50	-1.69E-46
MS.gene053998	MS.gene66939	0.820045	8.44E-53	-1.69E-46
MS.gene05417	MS.gene66939	0.804116	2.52E-49	-1.69E-46
MS.gene054236	MS.gene66939	0.838794	2.33E-57	-1.69E-46
MS.gene054467	MS.gene66939	0.807303	5.40E-50	-1.69E-46
MS.gene054651	MS.gene66939	0.835372	1.74E-56	-1.69E-46
MS.gene055227	MS.gene66939	0.824458	7.99E-54	-1.69E-46
MS.gene055228	MS.gene66939	0.805626	1.22E-49	-1.69E-46
MS.gene055818	MS.gene66939	0.817565	3.09E-52	-1.69E-46
MS.gene055926	MS.gene66939	0.822509	2.28E-53	-1.69E-46
MS.gene056174	MS.gene66939	0.835437	1.68E-56	-1.69E-46
MS.gene056553	MS.gene66939	0.825543	4.43E-54	-1.69E-46
MS.gene05686	MS.gene66939	0.823914	1.07E-53	-1.69E-46
MS.gene057208	MS.gene66939	0.800123	1.67E-48	-1.69E-46
MS.gene057314	MS.gene66939	0.805564	1.26E-49	-1.69E-46
MS.gene057598	MS.gene66939	0.802124	6.51E-49	-1.69E-46
MS.gene057869	MS.gene66939	0.820718	5.92E-53	-1.69E-46
MS.gene057915	MS.gene66939	0.821634	3.64E-53	-1.69E-46
MS.gene058009	MS.gene66939	0.801829	7.49E-49	-1.69E-46
MS.gene058010	MS.gene66939	0.849287	3.56E-60	-1.69E-46
MS.gene058037	MS.gene66939	0.802625	5.13E-49	-1.69E-46
MS.gene058625	MS.gene66939	0.801589	8.39E-49	-1.69E-46
MS.gene058979	MS.gene66939	0.831215	1.89E-55	-1.69E-46
MS.gene059842	MS.gene66939	0.818829	1.60E-52	-1.69E-46
MS.gene060011	MS.gene66939	0.813875	2.05E-51	-1.69E-46
MS.gene060167	MS.gene66939	0.815229	1.03E-51	-1.69E-46
MS.gene060310	MS.gene66939	0.813674	2.27E-51	-1.69E-46
MS.gene060312	MS.gene66939	0.814689	1.36E-51	-1.69E-46
MS.gene060322	MS.gene66939	0.808472	3.05E-50	-1.69E-46
MS.gene06052	MS.gene66939	0.804333	2.27E-49	-1.69E-46
MS.gene060998	MS.gene66939	0.809975	1.45E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66939.t1	MTR_3g096690	51.250	80	26	2	119	185	2	81	5.35e-16	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66939.t1	AT5G09250	47.692	65	26	1	131	187	19	83	7.14e-14	64.3
MS.gene66939.t1	AT5G09250	47.692	65	26	1	131	187	19	83	7.14e-14	64.3
MS.gene66939.t1	AT5G09250	49.206	63	24	1	131	185	19	81	1.18e-13	64.3
MS.gene66939.t1	AT5G09250	49.206	63	24	1	131	185	19	81	5.11e-13	63.2

Find 50 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGCGAATATTACTTAAAAGA+TGG	0.404513	6.2:-21709934	MS.gene66939:CDS
AGCATTGGAACGAAGGGATT+CGG	0.424874	6.2:-21709966	MS.gene66939:CDS
TTTATTACCAGGCTCAGTCT+AGG	0.432425	6.2:-21709591	None:intergenic
AATAGGTCTGGAACTGAAAA+GGG	0.449012	6.2:-21710176	MS.gene66939:CDS
GAAAAGGAGAAGAAGAGGTC+TGG	0.454761	6.2:-21710377	MS.gene66939:CDS
GAAAAGGAGAAGAAGAGGTC+TGG	0.454761	6.2:-21710485	MS.gene66939:CDS
GAAGAGGTCTGCAACTGAAA+AGG	0.458649	6.2:-21710204	MS.gene66939:CDS
GAAGAGGTCTGCAACTGAAA+AGG	0.458649	6.2:-21710258	MS.gene66939:CDS
GAAGAGGTCTGCAACTGAAA+AGG	0.458649	6.2:-21710312	MS.gene66939:CDS
GAAGAGGTCTGCAACTGAAA+AGG	0.458649	6.2:-21710420	MS.gene66939:CDS
CAACTGAAAAGGAGAAGAAT+AGG	0.461723	6.2:-21710193	MS.gene66939:CDS
CAACTGAAAAGGAGAAGAAT+AGG	0.461723	6.2:-21710247	MS.gene66939:CDS
CAACTGAAAAGGAGAAGAAT+AGG	0.461723	6.2:-21710301	MS.gene66939:CDS
CAACTGAAAAGGAGAAGAAT+AGG	0.461723	6.2:-21710409	MS.gene66939:CDS
GAATAGGTCTGGAACTGAAA+AGG	0.466779	6.2:-21710177	MS.gene66939:CDS
TTATTACCAGGCTCAGTCTA+GGG	0.468334	6.2:-21709590	None:intergenic
GAACTGAAAAGGAGAAGAAT+AGG	0.468877	6.2:-21710355	MS.gene66939:CDS
GAACTGAAAAGGAGAAGAAT+AGG	0.468877	6.2:-21710463	MS.gene66939:CDS
GAACTGAAAAGGAGAAGAAT+AGG	0.468877	6.2:-21710517	MS.gene66939:CDS
AGAAATGCCTGGAACTGAAA+AGG	0.469309	6.2:-21710528	None:intergenic
GAATAGGTCTGGAACTGAAA+AGG	0.472159	6.2:-21710231	MS.gene66939:CDS
GAATAGGTCTGGAACTGAAA+AGG	0.472159	6.2:-21710285	MS.gene66939:CDS
GAATAGGTCTGGAACTGAAA+AGG	0.472159	6.2:-21710339	MS.gene66939:CDS
GAATAGGTCTGGAACTGAAA+AGG	0.472159	6.2:-21710393	MS.gene66939:CDS
GAATAGGTCTGGAACTGAAA+AGG	0.472159	6.2:-21710447	MS.gene66939:CDS
GAATAGGTCTGGAACTGAAA+AGG	0.472159	6.2:-21710501	MS.gene66939:CDS
GAAAAGGAGAAGAATAGGTC+TGG	0.477457	6.2:-21710188	MS.gene66939:CDS
GAAAAGGAGAAGAATAGGTC+TGG	0.477457	6.2:-21710242	MS.gene66939:CDS
GAAAAGGAGAAGAATAGGTC+TGG	0.477457	6.2:-21710296	MS.gene66939:CDS
GAAAAGGAGAAGAATAGGTC+TGG	0.477457	6.2:-21710350	MS.gene66939:CDS
GAAAAGGAGAAGAATAGGTC+TGG	0.477457	6.2:-21710404	MS.gene66939:CDS
GAAAAGGAGAAGAATAGGTC+TGG	0.477457	6.2:-21710458	MS.gene66939:CDS
GAAAAGGAGAAGAATAGGTC+TGG	0.477457	6.2:-21710512	MS.gene66939:CDS
GGAAGGTTTCTGTGAAGCAT+TGG	0.486433	6.2:-21709981	MS.gene66939:CDS
GAAGAGGTCTGGAACTGAAA+AGG	0.498166	6.2:-21710366	MS.gene66939:CDS
GAAGAGGTCTGGAACTGAAA+AGG	0.498166	6.2:-21710474	MS.gene66939:CDS
AATCTTTATAGAGAAATGCC+TGG	0.524021	6.2:-21710539	None:intergenic
CTGTGAAGCATTGGAACGAA+GGG	0.525495	6.2:-21709972	MS.gene66939:CDS
GAACTGAAAAGGAGAAGAAG+AGG	0.574608	6.2:-21710220	MS.gene66939:CDS
GAACTGAAAAGGAGAAGAAG+AGG	0.574608	6.2:-21710274	MS.gene66939:CDS
GAACTGAAAAGGAGAAGAAG+AGG	0.574608	6.2:-21710328	MS.gene66939:CDS
GAACTGAAAAGGAGAAGAAG+AGG	0.574608	6.2:-21710382	MS.gene66939:CDS
GAACTGAAAAGGAGAAGAAG+AGG	0.574608	6.2:-21710436	MS.gene66939:CDS
GAACTGAAAAGGAGAAGAAG+AGG	0.574608	6.2:-21710490	MS.gene66939:CDS
CGACGAAATCGTCGTATGTG+AGG	0.579073	6.2:-21710099	MS.gene66939:intron
GAACTGAAAAGGGGAAGAAG+AGG	0.586147	6.2:-21710166	MS.gene66939:CDS
TCTGTGAAGCATTGGAACGA+AGG	0.589293	6.2:-21709973	MS.gene66939:CDS
GCAGCTTGGGACAAAGAGGA+AGG	0.602778	6.2:-21709998	MS.gene66939:intron
TAACGCAGCTTGGGACAAAG+AGG	0.608874	6.2:-21710002	MS.gene66939:intron
ATAGGTCTGGAACTGAAAAG+GGG	0.667488	6.2:-21710175	MS.gene66939:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTTCCATAAAATTGAAT+TGG	+	chr6.2:21710376-21710395	None:intergenic	15.0%
!!	AATCACTTTATATAAAGATA+AGG	+	chr6.2:21710283-21710302	None:intergenic	15.0%
!!	TTATGAAATGTTGTTATAAT+GGG	-	chr6.2:21710251-21710270	MS.gene66939:CDS	15.0%
!!	TTTATGAAATGTTGTTATAA+TGG	-	chr6.2:21710250-21710269	MS.gene66939:CDS	15.0%
!!!	ATTCAATTTTATGGAAATTT+TGG	-	chr6.2:21710376-21710395	MS.gene66939:CDS	15.0%
!!	CAAAATTGATAAACAAAATC+AGG	+	chr6.2:21710343-21710362	None:intergenic	20.0%
!!!	ATGAATTGAAATACCTTTTT+TGG	+	chr6.2:21710222-21710241	None:intergenic	20.0%
!!!	GACAAAGGTTTTTTAAAAAA+AGG	-	chr6.2:21710450-21710469	MS.gene66939:CDS	20.0%
!	ATGTTTAATGGAATAGCAAA+TGG	-	chr6.2:21710309-21710328	MS.gene66939:CDS	25.0%
!	GTGATTGATGAAATGTTTAA+TGG	-	chr6.2:21710297-21710316	MS.gene66939:CDS	25.0%
!!	TTGTCACCAATTCAATTTTA+TGG	-	chr6.2:21710367-21710386	MS.gene66939:CDS	25.0%
!!!	ATTGAAATACCTTTTTTGGT+AGG	+	chr6.2:21710218-21710237	None:intergenic	25.0%
	CGCGAATATTACTTAAAAGA+TGG	-	chr6.2:21710182-21710201	MS.gene66939:CDS	30.0%
	TGATGAAATTCAAAAGCCTA+AGG	-	chr6.2:21710425-21710444	MS.gene66939:CDS	30.0%
	AAAAAACCTTTGTCGTCCTT+AGG	+	chr6.2:21710444-21710463	None:intergenic	35.0%
	AAGCAAAGACCTACCAAAAA+AGG	-	chr6.2:21710206-21710225	MS.gene66939:CDS	35.0%
	ATGGAATAGCAAATGGACAT+TGG	-	chr6.2:21710316-21710335	MS.gene66939:CDS	35.0%
	CAACTGAAAAGGAGAAGAAT+AGG	-	chr6.2:21709707-21709726	MS.gene66939:intron	35.0%
	GAACTGAAAAGGAGAAGAAT+AGG	-	chr6.2:21709599-21709618	MS.gene66939:intron	35.0%
!	AATAGGTCTGGAACTGAAAA+GGG	-	chr6.2:21709616-21709635	MS.gene66939:intron	35.0%
!!	TGATTTGTTTAACGCAGCTT+GGG	-	chr6.2:21710105-21710124	MS.gene66939:CDS	35.0%
!!	TTGATTTGTTTAACGCAGCT+TGG	-	chr6.2:21710104-21710123	MS.gene66939:CDS	35.0%
	AGACTGAGCCTGGTAATAAA+GGG	+	chr6.2:21710525-21710544	None:intergenic	40.0%
	AGCCTGGTAATAAAGGGAAA+GGG	+	chr6.2:21710519-21710538	None:intergenic	40.0%
	GAAAAGGAGAAGAATAGGTC+TGG	-	chr6.2:21709604-21709623	MS.gene66939:intron	40.0%
	GAACTGAAAAGGAGAAGAAG+AGG	-	chr6.2:21709626-21709645	MS.gene66939:intron	40.0%
	GCAAAAAGAAACCCAAGCAA+TGG	+	chr6.2:21710066-21710085	None:intergenic	40.0%
	TAGACTGAGCCTGGTAATAA+AGG	+	chr6.2:21710526-21710545	None:intergenic	40.0%
	TTCCCTTTCCCTTTATTACC+AGG	-	chr6.2:21710514-21710533	MS.gene66939:CDS	40.0%
!	ATAGGTCTGGAACTGAAAAG+GGG	-	chr6.2:21709617-21709636	MS.gene66939:intron	40.0%
!	GAATAGGTCTGGAACTGAAA+AGG	-	chr6.2:21709615-21709634	MS.gene66939:intron	40.0%
!	TTCTTCTCCTTTTCAGTTCC+AGG	+	chr6.2:21709598-21709617	None:intergenic	40.0%
	AAACCCAAGCAATGGAGTTG+GGG	+	chr6.2:21710058-21710077	None:intergenic	45.0%
	AGAAACCCAAGCAATGGAGT+TGG	+	chr6.2:21710060-21710079	None:intergenic	45.0%
	CAAAAGCCTAAGGACGACAA+AGG	-	chr6.2:21710435-21710454	MS.gene66939:CDS	45.0%
	GAAAAGGAGAAGAAGAGGTC+TGG	-	chr6.2:21709631-21709650	MS.gene66939:intron	45.0%
	GAAACCCAAGCAATGGAGTT+GGG	+	chr6.2:21710059-21710078	None:intergenic	45.0%
	GAAATCGTCGTATGTGAGGT+CGG	-	chr6.2:21710021-21710040	MS.gene66939:intron	45.0%
	GAACTGAAAAGGGGAAGAAG+AGG	-	chr6.2:21709950-21709969	MS.gene66939:CDS	45.0%
	GAAGAGGTCTGCAACTGAAA+AGG	-	chr6.2:21709696-21709715	MS.gene66939:intron	45.0%
	GAGCCTGGTAATAAAGGGAA+AGG	+	chr6.2:21710520-21710539	None:intergenic	45.0%
	GGAAGGTTTCTGTGAAGCAT+TGG	-	chr6.2:21710135-21710154	MS.gene66939:CDS	45.0%
	TCACGCATCTCCCAAAAAGA+CGG	-	chr6.2:21709990-21710009	MS.gene66939:CDS	45.0%
!	AGCATTGGAACGAAGGGATT+CGG	-	chr6.2:21710150-21710169	MS.gene66939:CDS	45.0%
!	GAAGAGGTCTGGAACTGAAA+AGG	-	chr6.2:21709642-21709661	MS.gene66939:intron	45.0%
!	TCGTCGTTGACCGTCTTTTT+GGG	+	chr6.2:21710003-21710022	None:intergenic	45.0%
!	TTCGTCGTTGACCGTCTTTT+TGG	+	chr6.2:21710004-21710023	None:intergenic	45.0%
!!	CTGTGAAGCATTGGAACGAA+GGG	-	chr6.2:21710144-21710163	MS.gene66939:CDS	45.0%
!!	TCTGTGAAGCATTGGAACGA+AGG	-	chr6.2:21710143-21710162	MS.gene66939:CDS	45.0%
	AAACCCCCAACTCCATTGCT+TGG	-	chr6.2:21710051-21710070	MS.gene66939:intron	50.0%
	AACCCAAGCAATGGAGTTGG+GGG	+	chr6.2:21710057-21710076	None:intergenic	50.0%
	AACCCCCAACTCCATTGCTT+GGG	-	chr6.2:21710052-21710071	MS.gene66939:intron	50.0%
	CGACGAAATCGTCGTATGTG+AGG	-	chr6.2:21710017-21710036	MS.gene66939:intron	50.0%
	TAACGCAGCTTGGGACAAAG+AGG	-	chr6.2:21710114-21710133	MS.gene66939:CDS	50.0%
!	CAATGGAGTTGGGGGTTTTG+AGG	+	chr6.2:21710049-21710068	None:intergenic	50.0%
	GCAGCTTGGGACAAAGAGGA+AGG	-	chr6.2:21710118-21710137	MS.gene66939:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	21709592	21710546	21709592	ID=MS.gene66939
chr6.2	mRNA	21709592	21710546	21709592	ID=MS.gene66939.t1;Parent=MS.gene66939
chr6.2	exon	21710100	21710546	21710100	ID=MS.gene66939.t1.exon1;Parent=MS.gene66939.t1
chr6.2	CDS	21710100	21710546	21710100	ID=cds.MS.gene66939.t1;Parent=MS.gene66939.t1
chr6.2	exon	21709911	21710016	21709911	ID=MS.gene66939.t1.exon2;Parent=MS.gene66939.t1
chr6.2	CDS	21709911	21710016	21709911	ID=cds.MS.gene66939.t1;Parent=MS.gene66939.t1
chr6.2	exon	21709592	21709602	21709592	ID=MS.gene66939.t1.exon3;Parent=MS.gene66939.t1
chr6.2	CDS	21709592	21709602	21709592	ID=cds.MS.gene66939.t1;Parent=MS.gene66939.t1

Gene Sequence

>MS.gene66939

ATGCCTGGAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGAGGTCTGGAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGAGGTCTGCAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGAGGTCTGGAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGAGGTCTGCAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGAGGTCTGCAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGAGAAGAAGAGGTCTGCAACTGAAAAGGAGAAGAATAGGTCTGGAACTGAAAAGGGGAAGAAGAGGAAAGAACTTACTGAAGCTCACGCATCTCCCAAAAAGACGGTCAACGACGAAATCGTCGTATGTGAGGTCGGTTCCTCAAAACCCCCAACTCCATTGCTTGGGTTTCTTTTTGCTCTTTTTATTTATTGATATTGATTTGTTTAACGCAGCTTGGGACAAAGAGGAAGGTTTCTGTGAAGCATTGGAACGAAGGGATTCGGATTGACATTCGCGAATATTACTTAAAAGATGGCAAGCAAAGACCTACCAAAAAAGGTATTTCAATTCATTTTCGTTGTTTATGAAATGTTGTTATAATGGGTTGTTTCCTTATCTTTATATAAAGTGATTGATGAAATGTTTAATGGAATAGCAAATGGACATTGGACCTGATTTTGTTTATCAATTTTGAAAATTGTCACCAATTCAATTTTATGGAAATTTTGGTTAACACAACATTTTTTGCATCATGTTGATGAAATTCAAAAGCCTAAGGACGACAAAGGTTTTTTAAAAAAAGGTTTGTGAGCTGCATTTGTGTTGAAAATTTATGTTTGCTTGCTTCCCTTTCCCTTTATTACCAGGCTCAGTCTAG

Protein sequence

>MS.gene66939.t1

MPGTEKEKNRSGTEKEKKRSGTEKEKNRSGTEKEKKRSATEKEKNRSGTEKEKKRSGTEKEKNRSGTEKEKKRSATEKEKNRSGTEKEKKRSATEKEKNRSGTEKEKKRSATEKEKNRSGTEKGKKRKELTEAHASPKKTVNDEIVVCELGTKRKVSVKHWNEGIRIDIREYYLKDGKQRPTKKGSV