AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73014


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73014.t1 MTR_7g091460 65.854 164 53 1 1 161 783 946 3.32e-42 149
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73014.t1 AT2G41740 44.444 198 62 8 6 161 748 939 1.00e-20 88.2
MS.gene73014.t1 AT2G41740 44.444 198 62 8 6 161 748 939 1.00e-20 88.2
MS.gene73014.t1 AT3G57410 47.500 160 71 3 2 161 782 928 1.27e-16 76.6
MS.gene73014.t1 AT3G57410 47.500 160 71 3 2 161 782 928 1.27e-16 76.6
MS.gene73014.t1 AT3G57410 47.500 160 71 3 2 161 782 928 1.27e-16 76.6
MS.gene73014.t1 AT3G57410 47.500 160 71 3 2 161 782 928 1.27e-16 76.6
MS.gene73014.t1 AT3G57410 47.500 160 71 3 2 161 782 928 1.27e-16 76.6
MS.gene73014.t1 AT3G57410 47.500 160 71 3 2 161 782 928 1.27e-16 76.6
MS.gene73014.t1 AT3G57410 47.500 160 71 3 2 161 782 928 1.27e-16 76.6
MS.gene73014.t1 AT3G57410 47.500 160 71 3 2 161 782 928 1.27e-16 76.6

Find 35 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAACTAAATGCGGAGTTTA+AGG 0.252036 1.2:-26613605 None:intergenic
GTTCCAGAGAGTAGCATTTC+AGG 0.273762 1.2:+26613778 MS.gene73014:CDS
ACTCCTAGGCCATCTGTAAA+AGG 0.329533 1.2:+26613649 MS.gene73014:CDS
CTCCTGATTCCGATAGCAAT+GGG 0.368283 1.2:+26614593 MS.gene73014:CDS
CTCTTTGTCTTGGTCCTCCA+TGG 0.382592 1.2:-26613567 None:intergenic
AGAAACTTCTTCCACTTCCT+TGG 0.413003 1.2:-26614572 None:intergenic
TGTCTAGGATTGATCTTAAA+CGG 0.444135 1.2:+26614719 MS.gene73014:CDS
CTGGCCACAGGAGAGGTATC+AGG 0.449973 1.2:-26613745 None:intergenic
AAAGCTTCCGCTCTTTGTCT+TGG 0.456698 1.2:-26613577 None:intergenic
GATCAATTAAAGACTACATC+AGG 0.462111 1.2:+26614688 MS.gene73014:CDS
CAGTCACAAGAGAGATTGAA+TGG 0.470209 1.2:+26613538 None:intergenic
GAAATGCTACTCTCTGGAAC+AGG 0.494243 1.2:-26613775 None:intergenic
TTACCTGAAATGCTACTCTC+TGG 0.510871 1.2:-26613781 None:intergenic
CAACAAAGAAAGTTACTCCT+AGG 0.511920 1.2:+26613635 MS.gene73014:CDS
CTCTGGAACAGGACTGTCAC+TGG 0.517307 1.2:-26613764 None:intergenic
AGCTACAGAAGCCAAGGAAG+TGG 0.518580 1.2:+26614561 MS.gene73014:CDS
GGAATCTGAACAAGAATATG+TGG 0.523447 1.2:+26614621 MS.gene73014:CDS
TGATGAAGCTACAGAAGCCA+AGG 0.540644 1.2:+26614555 MS.gene73014:CDS
TGAATGTCCTCTGACTACTT+TGG 0.561085 1.2:-26614660 None:intergenic
GAACAAGAATATGTGGAGGA+TGG 0.562270 1.2:+26614628 MS.gene73014:CDS
AGATTGAATGGCTTAAACCA+TGG 0.565932 1.2:+26613550 MS.gene73014:CDS
TCTCCTGATTCCGATAGCAA+TGG 0.567747 1.2:+26614592 MS.gene73014:CDS
ACAGGACTGTCACTGGCCAC+AGG 0.587561 1.2:-26613757 None:intergenic
GATTGATCTTAAACGGAGAG+AGG 0.596333 1.2:+26614726 MS.gene73014:CDS
AGGCCATCTGTAAAAGGTCA+AGG 0.608414 1.2:+26613655 MS.gene73014:CDS
CGTCAGTTCTTACTGCTGAG+AGG 0.621045 1.2:+26613710 MS.gene73014:CDS
CCTGATTCCGATAGCAATGG+GGG 0.624831 1.2:+26614595 MS.gene73014:CDS
ACTGTCACTGGCCACAGGAG+AGG 0.635142 1.2:-26613752 None:intergenic
TCCTGATTCCGATAGCAATG+GGG 0.635451 1.2:+26614594 MS.gene73014:CDS
TGGAGGACCAAGACAAAGAG+CGG 0.641857 1.2:+26613570 MS.gene73014:CDS
ATCACCTGATACCTCTCCTG+TGG 0.671050 1.2:+26613741 MS.gene73014:CDS
ATAATGACCAAAGTAGTCAG+AGG 0.681044 1.2:+26614653 MS.gene73014:CDS
TGTTGATGAAGAACTAAATG+CGG 0.703179 1.2:-26613614 None:intergenic
ATCTGAACAAGAATATGTGG+AGG 0.712418 1.2:+26614624 MS.gene73014:CDS
TTGAATGGCTTAAACCATGG+AGG 0.721883 1.2:+26613553 MS.gene73014:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTGTTTAATTATTTCAA+TGG + chr1.2:26614215-26614234 MS.gene73014:intron 15.0%
!! GTAATAATCGAATAAACTAT+AGG + chr1.2:26614371-26614390 MS.gene73014:intron 20.0%
!!! TTTTTGCAACAAATAGAATA+TGG - chr1.2:26613865-26613884 None:intergenic 20.0%
! CCTAATTTACTTAGAGAATA+AGG - chr1.2:26614038-26614057 None:intergenic 25.0%
! CCTTATTCTCTAAGTAAATT+AGG + chr1.2:26614035-26614054 MS.gene73014:intron 25.0%
! TGATGTAATTTATTTGACCA+TGG - chr1.2:26614253-26614272 None:intergenic 25.0%
! TTATATAGAGTTAAGTAGCT+TGG - chr1.2:26614110-26614129 None:intergenic 25.0%
! TTCTTCTCTTCAAATTCAAA+AGG - chr1.2:26614344-26614363 None:intergenic 25.0%
AAAAGATTTACACAGACTGT+TGG - chr1.2:26613926-26613945 None:intergenic 30.0%
GATCAATTAAAGACTACATC+AGG + chr1.2:26614688-26614707 MS.gene73014:CDS 30.0%
! TGTCTAGGATTGATCTTAAA+CGG + chr1.2:26614719-26614738 MS.gene73014:CDS 30.0%
! TGTTGATGAAGAACTAAATG+CGG - chr1.2:26613617-26613636 None:intergenic 30.0%
!! AAATTCAAAAGGCACTCAAT+AGG - chr1.2:26614333-26614352 None:intergenic 30.0%
!!! GTCTGTGTAAATCTTTTTTG+TGG + chr1.2:26613929-26613948 MS.gene73014:intron 30.0%
ACAAATAGAATATGGACCAG+TGG - chr1.2:26613857-26613876 None:intergenic 35.0%
AGAACTAAATGCGGAGTTTA+AGG - chr1.2:26613608-26613627 None:intergenic 35.0%
AGATTGAATGGCTTAAACCA+TGG + chr1.2:26613550-26613569 MS.gene73014:CDS 35.0%
ATAATGACCAAAGTAGTCAG+AGG + chr1.2:26614653-26614672 MS.gene73014:CDS 35.0%
ATCTGAACAAGAATATGTGG+AGG + chr1.2:26614624-26614643 MS.gene73014:CDS 35.0%
CAACAAAGAAAGTTACTCCT+AGG + chr1.2:26613635-26613654 MS.gene73014:CDS 35.0%
CATACTCAAATTTCAACCAC+TGG + chr1.2:26613838-26613857 MS.gene73014:intron 35.0%
CATCAGGAAAAAATGTGTCT+AGG + chr1.2:26614704-26614723 MS.gene73014:CDS 35.0%
GGAATCTGAACAAGAATATG+TGG + chr1.2:26614621-26614640 MS.gene73014:CDS 35.0%
GTGTTTACACAACCATTTCT+GGG + chr1.2:26614462-26614481 MS.gene73014:intron 35.0%
! AATGGCTAGTATTTTGTCCA+TGG + chr1.2:26614233-26614252 MS.gene73014:intron 35.0%
! CTATAACGCGCTAATTTTTG+TGG + chr1.2:26614288-26614307 MS.gene73014:intron 35.0%
!!! TTTCGGCTTATTTTACCAGT+TGG + chr1.2:26614487-26614506 MS.gene73014:intron 35.0%
AGAAACTTCTTCCACTTCCT+TGG - chr1.2:26614575-26614594 None:intergenic 40.0%
CGTGTTTACACAACCATTTC+TGG + chr1.2:26614461-26614480 MS.gene73014:intron 40.0%
GAACAAGAATATGTGGAGGA+TGG + chr1.2:26614628-26614647 MS.gene73014:CDS 40.0%
GAGGAATCTTCATAGGCAAT+AGG + chr1.2:26614410-26614429 MS.gene73014:intron 40.0%
TGAATGTCCTCTGACTACTT+TGG - chr1.2:26614663-26614682 None:intergenic 40.0%
TTACCTGAAATGCTACTCTC+TGG - chr1.2:26613784-26613803 None:intergenic 40.0%
TTGAATGGCTTAAACCATGG+AGG + chr1.2:26613553-26613572 MS.gene73014:CDS 40.0%
TTGCCTATGAAGATTCCTCT+AGG - chr1.2:26614409-26614428 None:intergenic 40.0%
! ACAACCATTTCTGGGTGTTT+CGG + chr1.2:26614470-26614489 MS.gene73014:intron 40.0%
! CAATAGGCCAGGGTTTTTAA+AGG - chr1.2:26614317-26614336 None:intergenic 40.0%
! GATCCTTGACCTTTTACAGA+TGG - chr1.2:26613661-26613680 None:intergenic 40.0%
! GTGGATACCTTTAAAAACCC+TGG + chr1.2:26614307-26614326 MS.gene73014:intron 40.0%
AAAGCTTCCGCTCTTTGTCT+TGG - chr1.2:26613580-26613599 None:intergenic 45.0%
ACTCCTAGGCCATCTGTAAA+AGG + chr1.2:26613649-26613668 MS.gene73014:CDS 45.0%
AGGCCATCTGTAAAAGGTCA+AGG + chr1.2:26613655-26613674 MS.gene73014:CDS 45.0%
CCACCTAGAGGAATCTTCAT+AGG + chr1.2:26614403-26614422 MS.gene73014:intron 45.0%
CCTATGAAGATTCCTCTAGG+TGG - chr1.2:26614406-26614425 None:intergenic 45.0%
CTCCTGATTCCGATAGCAAT+GGG + chr1.2:26614593-26614612 MS.gene73014:CDS 45.0%
GTTCCAGAGAGTAGCATTTC+AGG + chr1.2:26613778-26613797 MS.gene73014:CDS 45.0%
TAAGCCGAAACACCCAGAAA+TGG - chr1.2:26614477-26614496 None:intergenic 45.0%
TCCTGATTCCGATAGCAATG+GGG + chr1.2:26614594-26614613 MS.gene73014:CDS 45.0%
TCTCCTGATTCCGATAGCAA+TGG + chr1.2:26614592-26614611 MS.gene73014:CDS 45.0%
TCTGCAGCAAAAACACCAAC+TGG - chr1.2:26614505-26614524 None:intergenic 45.0%
TGATGAAGCTACAGAAGCCA+AGG + chr1.2:26614555-26614574 MS.gene73014:CDS 45.0%
TGGTTGTGTAAACACGAGTC+AGG - chr1.2:26614457-26614476 None:intergenic 45.0%
! AGGCTTTTACTTCCACCTAG+AGG + chr1.2:26614391-26614410 MS.gene73014:intron 45.0%
! TGACCTTTTACAGATGGCCT+AGG - chr1.2:26613655-26613674 None:intergenic 45.0%
!! AAAAGGCACTCAATAGGCCA+GGG - chr1.2:26614327-26614346 None:intergenic 45.0%
!! GAAATGCTACTCTCTGGAAC+AGG - chr1.2:26613778-26613797 None:intergenic 45.0%
!! AAAAAAATAAAAAAATACAA+TGG + chr1.2:26613881-26613900 MS.gene73014:intron 5.0%
!! AAAAAATAAAAAAATACAAT+GGG + chr1.2:26613882-26613901 MS.gene73014:intron 5.0%
AGCTACAGAAGCCAAGGAAG+TGG + chr1.2:26614561-26614580 MS.gene73014:CDS 50.0%
ATCACCTGATACCTCTCCTG+TGG + chr1.2:26613741-26613760 MS.gene73014:CDS 50.0%
CCTGATTCCGATAGCAATGG+GGG + chr1.2:26614595-26614614 MS.gene73014:CDS 50.0%
CGTCAGTTCTTACTGCTGAG+AGG + chr1.2:26613710-26613729 MS.gene73014:CDS 50.0%
CTCTTTGTCTTGGTCCTCCA+TGG - chr1.2:26613570-26613589 None:intergenic 50.0%
TGGAGGACCAAGACAAAGAG+CGG + chr1.2:26613570-26613589 MS.gene73014:CDS 50.0%
TTCCAGACCCCCATTGCTAT+CGG - chr1.2:26614605-26614624 None:intergenic 50.0%
!! CAAAAGGCACTCAATAGGCC+AGG - chr1.2:26614328-26614347 None:intergenic 50.0%
CCCCCATTGCTATCGGAATC+AGG - chr1.2:26614598-26614617 None:intergenic 55.0%
TTCCGATAGCAATGGGGGTC+TGG + chr1.2:26614600-26614619 MS.gene73014:CDS 55.0%
!! CTCTGGAACAGGACTGTCAC+TGG - chr1.2:26613767-26613786 None:intergenic 55.0%
ACAGGACTGTCACTGGCCAC+AGG - chr1.2:26613760-26613779 None:intergenic 60.0%
ACTGTCACTGGCCACAGGAG+AGG - chr1.2:26613755-26613774 None:intergenic 60.0%
!! CTGGCCACAGGAGAGGTATC+AGG - chr1.2:26613748-26613767 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 26613541 26614747 26613541 ID=MS.gene73014
chr1.2 mRNA 26613541 26614747 26613541 ID=MS.gene73014.t1;Parent=MS.gene73014
chr1.2 exon 26613541 26613799 26613541 ID=MS.gene73014.t1.exon1;Parent=MS.gene73014.t1
chr1.2 CDS 26613541 26613799 26613541 ID=cds.MS.gene73014.t1;Parent=MS.gene73014.t1
chr1.2 exon 26614524 26614747 26614524 ID=MS.gene73014.t1.exon2;Parent=MS.gene73014.t1
chr1.2 CDS 26614524 26614747 26614524 ID=cds.MS.gene73014.t1;Parent=MS.gene73014.t1
Gene Sequence

>MS.gene73014

TCACAAGAGAGATTGAATGGCTTAAACCATGGAGGACCAAGACAAAGAGCGGAAGCTTTAGCTGCCTTAAACTCCGCATTTAGTTCTTCATCAACAACAAAGAAAGTTACTCCTAGGCCATCTGTAAAAGGTCAAGGATCACAAAGAGCAGCAGCTGTAGCTGCTCTTTCGTCAGTTCTTACTGCTGAGAGGAAGAAACAATCACCTGATACCTCTCCTGTGGCCAGTGACAGTCCTGTTCCAGAGAGTAGCATTTCAGGTAATAACTGCAGTTTTTTTTATCGAAGTTGAATGCTGCATACTCAAATTTCAACCACTGGTCCATATTCTATTTGTTGCAAAAAAAATAAAAAAATACAATGGGTTTCTTATGTCTCTCTTTCCAACAGTCTGTGTAAATCTTTTTTGTGGAATATTATATATGTTAAGTGCTTTGTTGATATTTATTGATTAAATAAATAGTAAATATTATATTTACATTGTAAATATATTTTCCTTATTCTCTAAGTAAATTAGGATAGATATTATGTAGAATATTTATTCTGTAAATATACATAGCTTAGTCTCCAAGCTACTTAACTCTATATAATAGACATTTCGTAGTGCTATTCAGTCACACGTTATTTAAGCATATGTCTCTCTTCTCTCTCATTCAACATGCTTAATCATAATTACAATTGTTTAATTATTTCAATGGCTAGTATTTTGTCCATGGTCAAATAAATTACATCAGAGTGAGATCTTGTGCTATAACGCGCTAATTTTTGTGGATACCTTTAAAAACCCTGGCCTATTGAGTGCCTTTTGAATTTGAAGAGAAGAAGCATCATGTAATAATCGAATAAACTATAGGCTTTTACTTCCACCTAGAGGAATCTTCATAGGCAATAGGCTGTGCTGCATTAAAAAAATGCCTGACTCGTGTTTACACAACCATTTCTGGGTGTTTCGGCTTATTTTACCAGTTGGTGTTTTTGCTGCAGAAGCTAAAAGTGAAAGTGAATATTCTGAAGTTGATGAAGCTACAGAAGCCAAGGAAGTGGAAGAAGTTTCTCCTGATTCCGATAGCAATGGGGGTCTGGAATCTGAACAAGAATATGTGGAGGATGGAGATAATGACCAAAGTAGTCAGAGGACATTCAGTTATGATCAATTAAAGACTACATCAGGAAAAAATGTGTCTAGGATTGATCTTAAACGGAGAGAG

Protein sequence

>MS.gene73014.t1

SQERLNGLNHGGPRQRAEALAALNSAFSSSSTTKKVTPRPSVKGQGSQRAAAVAALSSVLTAERKKQSPDTSPVASDSPVPESSISEAKSESEYSEVDEATEAKEVEEVSPDSDSNGGLESEQEYVEDGDNDQSSQRTFSYDQLKTTSGKNVSRIDLKRRE