Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73236.t1 | XP_013454931.1 | 97.3 | 220 | 6 | 0 | 1 | 220 | 1 | 220 | 3.70E-121 | 444.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73236.t1 | P32110 | 66.8 | 223 | 68 | 2 | 4 | 220 | 3 | 225 | 2.5e-81 | 303.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73236.t1 | A0A072UGV0 | 97.3 | 220 | 6 | 0 | 1 | 220 | 1 | 220 | 2.7e-121 | 444.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene73236 | 0.815514 | 8.89E-52 | -1.69E-46 |
| MS.gene051699 | MS.gene73236 | 0.832907 | 7.23E-56 | -1.69E-46 |
| MS.gene051832 | MS.gene73236 | 0.826756 | 2.28E-54 | -1.69E-46 |
| MS.gene051959 | MS.gene73236 | 0.864773 | 9.55E-65 | -1.69E-46 |
| MS.gene051960 | MS.gene73236 | 0.856994 | 2.21E-62 | -1.69E-46 |
| MS.gene051979 | MS.gene73236 | 0.803794 | 2.94E-49 | -1.69E-46 |
| MS.gene052040 | MS.gene73236 | 0.812933 | 3.30E-51 | -1.69E-46 |
| MS.gene052053 | MS.gene73236 | 0.843426 | 1.41E-58 | -1.69E-46 |
| MS.gene052533 | MS.gene73236 | 0.813713 | 2.23E-51 | -1.69E-46 |
| MS.gene052559 | MS.gene73236 | 0.882406 | 1.07E-70 | -1.69E-46 |
| MS.gene052560 | MS.gene73236 | 0.829935 | 3.90E-55 | -1.69E-46 |
| MS.gene052570 | MS.gene73236 | 0.807021 | 6.19E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene73236 | 0.823564 | 1.29E-53 | -1.69E-46 |
| MS.gene054222 | MS.gene73236 | 0.809438 | 1.89E-50 | -1.69E-46 |
| MS.gene054490 | MS.gene73236 | 0.815813 | 7.63E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene73236 | 0.844708 | 6.40E-59 | -1.69E-46 |
| MS.gene054893 | MS.gene73236 | 0.842542 | 2.43E-58 | -1.69E-46 |
| MS.gene054957 | MS.gene73236 | 0.807532 | 4.83E-50 | -1.69E-46 |
| MS.gene055147 | MS.gene73236 | 0.808695 | 2.73E-50 | -1.69E-46 |
| MS.gene055148 | MS.gene73236 | 0.808422 | 3.12E-50 | -1.69E-46 |
| MS.gene055149 | MS.gene73236 | 0.812483 | 4.15E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene73236 | 0.848743 | 5.05E-60 | -1.69E-46 |
| MS.gene05624 | MS.gene73236 | 0.865827 | 4.45E-65 | -1.69E-46 |
| MS.gene056530 | MS.gene73236 | 0.834257 | 3.33E-56 | -1.69E-46 |
| MS.gene056677 | MS.gene73236 | 0.831049 | 2.08E-55 | -1.69E-46 |
| MS.gene056982 | MS.gene73236 | 0.80295 | 4.40E-49 | -1.69E-46 |
| MS.gene057001 | MS.gene73236 | 0.805328 | 1.41E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene73236 | 0.825199 | 5.34E-54 | -1.69E-46 |
| MS.gene057622 | MS.gene73236 | 0.812583 | 3.94E-51 | -1.69E-46 |
| MS.gene058095 | MS.gene73236 | 0.819803 | 9.59E-53 | -1.69E-46 |
| MS.gene058592 | MS.gene73236 | 0.864702 | 1.01E-64 | -1.69E-46 |
| MS.gene05878 | MS.gene73236 | 0.83605 | 1.17E-56 | -1.69E-46 |
| MS.gene05952 | MS.gene73236 | 0.872378 | 3.33E-67 | -1.69E-46 |
| MS.gene05956 | MS.gene73236 | 0.807426 | 5.08E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene73236 | -0.835599 | 1.53E-56 | -1.69E-46 |
| MS.gene059901 | MS.gene73236 | -0.818687 | 1.72E-52 | -1.69E-46 |
| MS.gene06023 | MS.gene73236 | 0.844621 | 6.75E-59 | -1.69E-46 |
| MS.gene060412 | MS.gene73236 | 0.810709 | 1.01E-50 | -1.69E-46 |
| MS.gene060964 | MS.gene73236 | 0.803253 | 3.81E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73236.t1 | MTR_4g019780 | 97.273 | 220 | 6 | 0 | 1 | 220 | 1 | 220 | 2.09e-156 | 432 |
| MS.gene73236.t1 | MTR_7g065720 | 63.303 | 218 | 76 | 3 | 1 | 216 | 1 | 216 | 1.38e-97 | 283 |
| MS.gene73236.t1 | MTR_7g065660 | 61.751 | 217 | 80 | 3 | 1 | 216 | 1 | 215 | 5.74e-95 | 277 |
| MS.gene73236.t1 | MTR_7g065590 | 61.033 | 213 | 81 | 2 | 5 | 216 | 4 | 215 | 5.29e-94 | 274 |
| MS.gene73236.t1 | MTR_4g019790 | 60.094 | 213 | 83 | 2 | 5 | 216 | 4 | 215 | 6.10e-94 | 274 |
| MS.gene73236.t1 | MTR_7g065740 | 60.092 | 218 | 83 | 3 | 1 | 216 | 1 | 216 | 2.48e-93 | 273 |
| MS.gene73236.t1 | MTR_7g065290 | 60.000 | 215 | 84 | 2 | 3 | 216 | 2 | 215 | 6.33e-93 | 271 |
| MS.gene73236.t1 | MTR_7g065600 | 60.280 | 214 | 83 | 2 | 4 | 216 | 3 | 215 | 2.74e-92 | 270 |
| MS.gene73236.t1 | MTR_7g065630 | 59.817 | 219 | 82 | 3 | 1 | 218 | 18 | 231 | 3.58e-91 | 268 |
| MS.gene73236.t1 | MTR_7g065750 | 59.259 | 216 | 85 | 2 | 3 | 216 | 2 | 216 | 1.06e-90 | 266 |
| MS.gene73236.t1 | MTR_7g065270 | 57.209 | 215 | 90 | 2 | 3 | 216 | 2 | 215 | 4.05e-88 | 259 |
| MS.gene73236.t1 | MTR_7g065265 | 58.140 | 215 | 88 | 2 | 3 | 216 | 2 | 215 | 8.25e-88 | 258 |
| MS.gene73236.t1 | MTR_7g065230 | 58.605 | 215 | 82 | 3 | 3 | 216 | 2 | 210 | 8.57e-86 | 253 |
| MS.gene73236.t1 | MTR_7g065260 | 57.339 | 218 | 91 | 2 | 4 | 220 | 10 | 226 | 3.23e-81 | 242 |
| MS.gene73236.t1 | MTR_7g065640 | 55.300 | 217 | 76 | 3 | 1 | 216 | 1 | 197 | 1.47e-80 | 239 |
| MS.gene73236.t1 | MTR_7g065710 | 54.327 | 208 | 89 | 3 | 9 | 215 | 5 | 207 | 3.66e-77 | 231 |
| MS.gene73236.t1 | MTR_7g065680 | 56.422 | 218 | 89 | 3 | 3 | 219 | 2 | 214 | 6.76e-75 | 226 |
| MS.gene73236.t1 | MTR_7g065700 | 56.542 | 214 | 87 | 3 | 3 | 215 | 2 | 210 | 3.70e-74 | 224 |
| MS.gene73236.t1 | MTR_6g080440 | 49.758 | 207 | 104 | 0 | 8 | 214 | 3 | 209 | 2.64e-72 | 219 |
| MS.gene73236.t1 | MTR_1g090150 | 46.226 | 212 | 111 | 2 | 6 | 215 | 1 | 211 | 1.34e-68 | 210 |
| MS.gene73236.t1 | MTR_5g040430 | 48.372 | 215 | 105 | 2 | 8 | 216 | 7 | 221 | 1.81e-66 | 204 |
| MS.gene73236.t1 | MTR_8g087425 | 48.585 | 212 | 109 | 0 | 5 | 216 | 2 | 213 | 6.57e-66 | 203 |
| MS.gene73236.t1 | MTR_3g099757 | 48.815 | 211 | 108 | 0 | 6 | 216 | 3 | 213 | 1.11e-65 | 202 |
| MS.gene73236.t1 | MTR_8g087410 | 48.325 | 209 | 108 | 0 | 5 | 213 | 3 | 211 | 5.61e-63 | 196 |
| MS.gene73236.t1 | MTR_1g115195 | 46.860 | 207 | 107 | 2 | 8 | 211 | 3 | 209 | 9.93e-61 | 189 |
| MS.gene73236.t1 | MTR_4g059730 | 45.161 | 217 | 115 | 3 | 7 | 220 | 3 | 218 | 1.04e-58 | 184 |
| MS.gene73236.t1 | MTR_1g090070 | 43.396 | 212 | 115 | 3 | 8 | 215 | 9 | 219 | 2.11e-56 | 179 |
| MS.gene73236.t1 | MTR_1g090060 | 43.602 | 211 | 113 | 3 | 8 | 214 | 3 | 211 | 5.85e-56 | 177 |
| MS.gene73236.t1 | MTR_1g090100 | 44.444 | 216 | 114 | 3 | 9 | 220 | 3 | 216 | 2.40e-54 | 173 |
| MS.gene73236.t1 | MTR_1g090090 | 43.333 | 210 | 113 | 3 | 8 | 213 | 3 | 210 | 7.92e-54 | 172 |
| MS.gene73236.t1 | MTR_2g070130 | 40.488 | 205 | 119 | 2 | 7 | 210 | 3 | 205 | 2.30e-49 | 160 |
| MS.gene73236.t1 | MTR_5g037380 | 40.088 | 227 | 125 | 4 | 1 | 219 | 1 | 224 | 4.94e-48 | 157 |
| MS.gene73236.t1 | MTR_2g070150 | 41.951 | 205 | 114 | 3 | 7 | 209 | 3 | 204 | 5.07e-48 | 157 |
| MS.gene73236.t1 | MTR_8g061950 | 39.091 | 220 | 124 | 5 | 6 | 216 | 3 | 221 | 3.12e-46 | 153 |
| MS.gene73236.t1 | MTR_2g070180 | 39.024 | 205 | 122 | 2 | 7 | 210 | 3 | 205 | 2.18e-45 | 150 |
| MS.gene73236.t1 | MTR_2g070120 | 37.321 | 209 | 128 | 2 | 7 | 214 | 3 | 209 | 2.39e-45 | 150 |
| MS.gene73236.t1 | MTR_2g070140 | 40.722 | 194 | 110 | 3 | 22 | 213 | 2 | 192 | 3.29e-44 | 147 |
| MS.gene73236.t1 | MTR_2g070200 | 40.367 | 218 | 123 | 6 | 7 | 220 | 3 | 217 | 4.73e-44 | 147 |
| MS.gene73236.t1 | MTR_2g070210 | 39.048 | 210 | 123 | 3 | 7 | 213 | 3 | 210 | 1.29e-43 | 146 |
| MS.gene73236.t1 | MTR_3g467430 | 40.465 | 215 | 115 | 7 | 5 | 213 | 6 | 213 | 1.63e-43 | 145 |
| MS.gene73236.t1 | MTR_2g070070 | 37.981 | 208 | 124 | 4 | 8 | 213 | 4 | 208 | 4.46e-43 | 144 |
| MS.gene73236.t1 | MTR_3g467420 | 38.942 | 208 | 120 | 5 | 9 | 213 | 10 | 213 | 8.16e-43 | 144 |
| MS.gene73236.t1 | MTR_4g124130 | 38.532 | 218 | 128 | 5 | 6 | 220 | 2 | 216 | 1.14e-42 | 144 |
| MS.gene73236.t1 | MTR_8g056940 | 37.321 | 209 | 126 | 4 | 7 | 213 | 3 | 208 | 1.77e-41 | 140 |
| MS.gene73236.t1 | MTR_2g070060 | 37.500 | 208 | 125 | 4 | 8 | 213 | 4 | 208 | 2.20e-41 | 140 |
| MS.gene73236.t1 | MTR_2g070110 | 37.198 | 207 | 126 | 3 | 8 | 213 | 4 | 207 | 5.25e-40 | 137 |
| MS.gene73236.t1 | MTR_5g076900 | 36.538 | 208 | 129 | 2 | 7 | 213 | 5 | 210 | 3.23e-39 | 135 |
| MS.gene73236.t1 | MTR_0186s0030 | 44.286 | 140 | 75 | 3 | 7 | 144 | 3 | 141 | 1.56e-33 | 117 |
| MS.gene73236.t1 | MTR_2g072120 | 47.297 | 74 | 37 | 2 | 52 | 124 | 2 | 74 | 2.23e-18 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73236.t1 | AT3G09270 | 58.140 | 215 | 87 | 3 | 5 | 216 | 2 | 216 | 2.81e-90 | 265 |
| MS.gene73236.t1 | AT2G29420 | 48.387 | 217 | 112 | 0 | 4 | 220 | 5 | 221 | 4.56e-77 | 231 |
| MS.gene73236.t1 | AT2G29450 | 49.282 | 209 | 104 | 2 | 3 | 209 | 1 | 209 | 1.06e-66 | 205 |
| MS.gene73236.t1 | AT2G29490 | 49.029 | 206 | 104 | 1 | 5 | 209 | 4 | 209 | 1.15e-66 | 205 |
| MS.gene73236.t1 | AT2G29460 | 44.240 | 217 | 120 | 1 | 5 | 220 | 4 | 220 | 4.73e-65 | 201 |
| MS.gene73236.t1 | AT2G29480 | 46.829 | 205 | 108 | 1 | 6 | 209 | 5 | 209 | 3.07e-64 | 199 |
| MS.gene73236.t1 | AT2G29440 | 44.495 | 218 | 120 | 1 | 4 | 220 | 2 | 219 | 8.00e-63 | 195 |
| MS.gene73236.t1 | AT2G29470 | 45.146 | 206 | 111 | 2 | 6 | 209 | 5 | 210 | 6.49e-60 | 188 |
| MS.gene73236.t1 | AT1G78320 | 42.654 | 211 | 110 | 7 | 6 | 211 | 2 | 206 | 3.70e-51 | 165 |
| MS.gene73236.t1 | AT1G59670 | 41.364 | 220 | 121 | 4 | 4 | 215 | 2 | 221 | 3.77e-50 | 163 |
| MS.gene73236.t1 | AT1G59700 | 43.182 | 220 | 117 | 4 | 4 | 215 | 2 | 221 | 5.91e-50 | 162 |
| MS.gene73236.t1 | AT1G74590 | 38.739 | 222 | 127 | 4 | 6 | 220 | 5 | 224 | 3.02e-49 | 160 |
| MS.gene73236.t1 | AT5G62480 | 38.182 | 220 | 127 | 2 | 6 | 216 | 6 | 225 | 3.64e-49 | 161 |
| MS.gene73236.t1 | AT1G27130 | 38.636 | 220 | 127 | 4 | 4 | 215 | 2 | 221 | 1.30e-48 | 159 |
| MS.gene73236.t1 | AT1G17170 | 40.000 | 215 | 125 | 3 | 7 | 220 | 3 | 214 | 1.90e-48 | 158 |
| MS.gene73236.t1 | AT1G78340 | 41.509 | 212 | 113 | 6 | 7 | 213 | 3 | 208 | 5.36e-48 | 157 |
| MS.gene73236.t1 | AT1G10370 | 39.171 | 217 | 125 | 2 | 6 | 215 | 3 | 219 | 5.62e-47 | 155 |
| MS.gene73236.t1 | AT1G53680 | 41.063 | 207 | 117 | 3 | 8 | 211 | 7 | 211 | 2.91e-46 | 153 |
| MS.gene73236.t1 | AT1G17180 | 39.810 | 211 | 118 | 4 | 7 | 213 | 3 | 208 | 9.27e-46 | 151 |
| MS.gene73236.t1 | AT1G78370 | 36.765 | 204 | 126 | 2 | 11 | 213 | 7 | 208 | 4.42e-45 | 149 |
| MS.gene73236.t1 | AT1G69930 | 35.945 | 217 | 133 | 3 | 3 | 213 | 7 | 223 | 2.15e-44 | 148 |
| MS.gene73236.t1 | AT1G78380 | 37.681 | 207 | 126 | 2 | 8 | 213 | 4 | 208 | 9.12e-43 | 144 |
| MS.gene73236.t1 | AT1G10360 | 37.333 | 225 | 125 | 7 | 1 | 215 | 1 | 219 | 3.98e-42 | 142 |
| MS.gene73236.t1 | AT1G17190 | 36.967 | 211 | 124 | 6 | 7 | 213 | 4 | 209 | 4.71e-41 | 139 |
| MS.gene73236.t1 | AT1G69920 | 35.909 | 220 | 133 | 4 | 4 | 215 | 30 | 249 | 9.65e-41 | 139 |
| MS.gene73236.t1 | AT3G43800 | 36.893 | 206 | 123 | 4 | 5 | 205 | 2 | 205 | 8.00e-40 | 136 |
| MS.gene73236.t1 | AT1G78360 | 36.842 | 209 | 126 | 4 | 8 | 213 | 4 | 209 | 9.15e-38 | 131 |
| MS.gene73236.t1 | AT1G27140 | 34.685 | 222 | 134 | 5 | 4 | 215 | 2 | 222 | 2.71e-36 | 128 |
| MS.gene73236.t1 | AT1G78320 | 36.667 | 210 | 97 | 7 | 6 | 211 | 2 | 179 | 3.29e-36 | 126 |
| MS.gene73236.t1 | AT5G62480 | 31.050 | 219 | 118 | 2 | 6 | 216 | 6 | 199 | 7.27e-32 | 115 |
| MS.gene73236.t1 | AT5G62480 | 31.050 | 219 | 117 | 2 | 6 | 216 | 6 | 198 | 1.38e-31 | 115 |
Find 48 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACTCCATTTATATATCTT+TGG | 0.228697 | 4.1:+78430850 | None:intergenic |
| CTGCCAATTTCATTGGTTAT+TGG | 0.261177 | 4.1:-78430927 | MS.gene73236:CDS |
| TAGTATCTTTAGAGCTTCTA+AGG | 0.264171 | 4.1:+78431011 | None:intergenic |
| TGAAGCTGAAGCTTTGAAAA+AGG | 0.266929 | 4.1:+78430768 | None:intergenic |
| AAGTGAGGCTATTAGGAAAA+TGG | 0.276631 | 4.1:-78432397 | MS.gene73236:CDS |
| CAAGAAGAAGTGAGGCTATT+AGG | 0.278486 | 4.1:-78432404 | MS.gene73236:CDS |
| AACCCAATAACCAATGAAAT+TGG | 0.291512 | 4.1:+78430924 | None:intergenic |
| TGCCAATTTCATTGGTTATT+GGG | 0.296047 | 4.1:-78430926 | MS.gene73236:CDS |
| AAAGAGCCTTGGCTCGGTTT+TGG | 0.299606 | 4.1:-78432139 | MS.gene73236:CDS |
| ATAGGAACCTTCTTGTAAAC+AGG | 0.305933 | 4.1:+78432263 | None:intergenic |
| ATAAAGGACAAGAAATTCTT+TGG | 0.317481 | 4.1:-78430979 | MS.gene73236:CDS |
| AGATCAATTCTGTTGCTAAA+TGG | 0.322966 | 4.1:+78432368 | None:intergenic |
| GGCAACCCTTCCTTGATAAC+AGG | 0.336300 | 4.1:+78430811 | None:intergenic |
| GACATTGCTGCCAATTTCAT+TGG | 0.358867 | 4.1:-78430934 | MS.gene73236:CDS |
| CTGCAATATGGAATGCTTGC+TGG | 0.367214 | 4.1:-78431071 | MS.gene73236:CDS |
| GATACTAGAGGATGAAATAA+AGG | 0.371068 | 4.1:-78430995 | MS.gene73236:CDS |
| TTTCCATTGTGAACAAGAAT+AGG | 0.380782 | 4.1:+78432245 | None:intergenic |
| AATTTCCAAAGATATATAAA+TGG | 0.386278 | 4.1:-78430855 | MS.gene73236:CDS |
| CAGCAAGCATTCCATATTGC+AGG | 0.405973 | 4.1:+78431072 | None:intergenic |
| GATATATTACAAGAAATTGC+AGG | 0.426457 | 4.1:-78430901 | MS.gene73236:CDS |
| ATGATAAGAGACTCAGCAAT+TGG | 0.431930 | 4.1:+78432221 | None:intergenic |
| AGTGAGGCTATTAGGAAAAT+GGG | 0.434579 | 4.1:-78432396 | MS.gene73236:CDS |
| TATCAATCACCCTGTTATCA+AGG | 0.446058 | 4.1:-78430821 | MS.gene73236:CDS |
| AAGGCAAATAGTTCATCTCT+TGG | 0.450565 | 4.1:+78430787 | None:intergenic |
| CGAGCCAAGGCTCTTTCATA+AGG | 0.450913 | 4.1:+78432146 | None:intergenic |
| TTCTTTGGTGGAGAAACCTT+AGG | 0.484651 | 4.1:-78430964 | MS.gene73236:CDS |
| GATCTTGCTTTAAAACTCAA+AGG | 0.505899 | 4.1:-78432350 | MS.gene73236:CDS |
| AATCACCCTGTTATCAAGGA+AGG | 0.524655 | 4.1:-78430817 | MS.gene73236:CDS |
| TAAGGATCTTGAGACAAAAG+TGG | 0.532667 | 4.1:+78432164 | None:intergenic |
| ATACAACCCTGTTTACAAGA+AGG | 0.537611 | 4.1:-78432270 | MS.gene73236:CDS |
| GTCTAAGACTCTTGATGACA+AGG | 0.547006 | 4.1:-78432117 | MS.gene73236:intron |
| AAGGACAAGAAATTCTTTGG+TGG | 0.547467 | 4.1:-78430976 | MS.gene73236:CDS |
| GTTCCTATTCTTGTTCACAA+TGG | 0.548341 | 4.1:-78432248 | MS.gene73236:CDS |
| GCAAATAGTTCATCTCTTGG+AGG | 0.560186 | 4.1:+78430790 | None:intergenic |
| AGCAATGTCTACAAGTCCTA+AGG | 0.566666 | 4.1:+78430948 | None:intergenic |
| CTTATGAAAGAGCCTTGGCT+CGG | 0.573191 | 4.1:-78432145 | MS.gene73236:CDS |
| AGATCCTTATGAAAGAGCCT+TGG | 0.584406 | 4.1:-78432150 | MS.gene73236:CDS |
| ATATATTACAAGAAATTGCA+GGG | 0.589528 | 4.1:-78430900 | MS.gene73236:CDS |
| TTGAATACATAGATGATACA+TGG | 0.594386 | 4.1:-78432196 | MS.gene73236:CDS |
| AGGACGTGAGAAAGCTGTGG+AGG | 0.607732 | 4.1:-78431037 | MS.gene73236:CDS |
| ATCACCCTGTTATCAAGGAA+GGG | 0.614487 | 4.1:-78430816 | MS.gene73236:CDS |
| GCTTGCTGGAGTGATGAGAA+AGG | 0.621777 | 4.1:-78431057 | MS.gene73236:CDS |
| TAGGAACCTTCTTGTAAACA+GGG | 0.624629 | 4.1:+78432264 | None:intergenic |
| CTTAGACCAAAACCGAGCCA+AGG | 0.650325 | 4.1:+78432133 | None:intergenic |
| GCAACCCTTCCTTGATAACA+GGG | 0.651245 | 4.1:+78430812 | None:intergenic |
| CTGCAAATCAAGAAGAAGTG+AGG | 0.653676 | 4.1:-78432412 | MS.gene73236:CDS |
| GAAAGGACGTGAGAAAGCTG+TGG | 0.660199 | 4.1:-78431040 | MS.gene73236:CDS |
| AGAAGCTCTAAAGATACTAG+AGG | 0.667533 | 4.1:-78431007 | MS.gene73236:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTCCAAAGATATATAAA+TGG | - | chr4.1:78432326-78432345 | MS.gene73236:CDS | 15.0% |
| !! | TTTGTCTTTAATATACTATT+TGG | + | chr4.1:78432018-78432037 | None:intergenic | 15.0% |
| !!! | AAAAATAGGTTGAAATATTT+TGG | + | chr4.1:78431419-78431438 | None:intergenic | 15.0% |
| !!! | AAAATATTTCAACCTATTTT+TGG | - | chr4.1:78431418-78431437 | MS.gene73236:intron | 15.0% |
| !!! | TAATGTAAAATGATTTTGTT+TGG | + | chr4.1:78431576-78431595 | None:intergenic | 15.0% |
| !!! | TTACATAAGATTTTTCTTTT+TGG | - | chr4.1:78432074-78432093 | MS.gene73236:intron | 15.0% |
| !! | AAGAAAAATAAGTGTATGTT+TGG | - | chr4.1:78431191-78431210 | MS.gene73236:intron | 20.0% |
| !! | ATATATTACAAGAAATTGCA+GGG | - | chr4.1:78432281-78432300 | MS.gene73236:CDS | 20.0% |
| !!! | GTTGTTTTTTTTATTGGAAA+TGG | - | chr4.1:78431276-78431295 | MS.gene73236:intron | 20.0% |
| !!! | GTTTGTGTTGTTTTTTTTAT+TGG | - | chr4.1:78431270-78431289 | MS.gene73236:intron | 20.0% |
| ! | ATAAAGGACAAGAAATTCTT+TGG | - | chr4.1:78432202-78432221 | MS.gene73236:CDS | 25.0% |
| ! | GATATATTACAAGAAATTGC+AGG | - | chr4.1:78432280-78432299 | MS.gene73236:CDS | 25.0% |
| ! | TGACTCCATTTATATATCTT+TGG | + | chr4.1:78432334-78432353 | None:intergenic | 25.0% |
| ! | TTGAATACATAGATGATACA+TGG | - | chr4.1:78430985-78431004 | MS.gene73236:CDS | 25.0% |
| !!! | ATATTTCAACCTATTTTTGG+AGG | - | chr4.1:78431421-78431440 | MS.gene73236:intron | 25.0% |
| !!! | ATTAAAGACAAATTTGCTAG+TGG | - | chr4.1:78432026-78432045 | MS.gene73236:intron | 25.0% |
| !!! | CTATTTTTGGAGGTTTTATA+GGG | - | chr4.1:78431431-78431450 | MS.gene73236:intron | 25.0% |
| !!! | GTATGTTTGGATTCATTTTT+AGG | - | chr4.1:78431204-78431223 | MS.gene73236:intron | 25.0% |
| !!! | TTTGGATTCATTTTTAGGAT+AGG | - | chr4.1:78431209-78431228 | MS.gene73236:intron | 25.0% |
| !!! | TTTTCTTCAGAGTATTTGTA+TGG | + | chr4.1:78430861-78430880 | None:intergenic | 25.0% |
| AACCCAATAACCAATGAAAT+TGG | + | chr4.1:78432260-78432279 | None:intergenic | 30.0% | |
| ACTAAAATTCGAACTGCATA+AGG | - | chr4.1:78431472-78431491 | MS.gene73236:intron | 30.0% | |
| AGATCAATTCTGTTGCTAAA+TGG | + | chr4.1:78430816-78430835 | None:intergenic | 30.0% | |
| ATGTATCTGCTACAACAATT+TGG | + | chr4.1:78431974-78431993 | None:intergenic | 30.0% | |
| ATTGTCCAACAATTCTAAGT+TGG | + | chr4.1:78431774-78431793 | None:intergenic | 30.0% | |
| CAACAATTTGGTCGTTATTA+AGG | + | chr4.1:78431962-78431981 | None:intergenic | 30.0% | |
| CAACTACAAATCCTAATATC+AGG | + | chr4.1:78431316-78431335 | None:intergenic | 30.0% | |
| CATCAAAAATCTTGATGCAA+CGG | - | chr4.1:78431991-78432010 | MS.gene73236:intron | 30.0% | |
| CCTATAAAACCTCCAAAAAT+AGG | + | chr4.1:78431433-78431452 | None:intergenic | 30.0% | |
| GATACTAGAGGATGAAATAA+AGG | - | chr4.1:78432186-78432205 | MS.gene73236:CDS | 30.0% | |
| TAGAATTGTTGGACAATCAT+TGG | - | chr4.1:78431777-78431796 | MS.gene73236:intron | 30.0% | |
| TGTCATTCTAACCTGATATT+AGG | - | chr4.1:78431302-78431321 | MS.gene73236:intron | 30.0% | |
| TTGTCCAACAATTCTAAGTT+GGG | + | chr4.1:78431773-78431792 | None:intergenic | 30.0% | |
| TTTCCATTGTGAACAAGAAT+AGG | + | chr4.1:78430939-78430958 | None:intergenic | 30.0% | |
| ! | CTAATCTAGCTCATTCAAAT+TGG | + | chr4.1:78431909-78431928 | None:intergenic | 30.0% |
| ! | TATAGGGTTTTTATGCAGTT+CGG | - | chr4.1:78431447-78431466 | MS.gene73236:intron | 30.0% |
| ! | TGCCAATTTCATTGGTTATT+GGG | - | chr4.1:78432255-78432274 | MS.gene73236:CDS | 30.0% |
| ! | TTATGATAGCATTGAAGTAG+AGG | - | chr4.1:78431138-78431157 | MS.gene73236:intron | 30.0% |
| ! | TTTAGATTTTGCCTGCAATA+TGG | - | chr4.1:78432098-78432117 | MS.gene73236:intron | 30.0% |
| !! | GATCTTGCTTTAAAACTCAA+AGG | - | chr4.1:78430831-78430850 | MS.gene73236:CDS | 30.0% |
| !! | TAGTATCTTTAGAGCTTCTA+AGG | + | chr4.1:78432173-78432192 | None:intergenic | 30.0% |
| !!! | CCTATTTTTGGAGGTTTTAT+AGG | - | chr4.1:78431430-78431449 | MS.gene73236:intron | 30.0% |
| AAGGACAAGAAATTCTTTGG+TGG | - | chr4.1:78432205-78432224 | MS.gene73236:CDS | 35.0% | |
| AAGGCAAATAGTTCATCTCT+TGG | + | chr4.1:78432397-78432416 | None:intergenic | 35.0% | |
| AAGTGAGGCTATTAGGAAAA+TGG | - | chr4.1:78430784-78430803 | MS.gene73236:CDS | 35.0% | |
| AGTGAGGCTATTAGGAAAAT+GGG | - | chr4.1:78430785-78430804 | MS.gene73236:CDS | 35.0% | |
| AGTTTCTCGTGCATTCTATT+AGG | - | chr4.1:78431383-78431402 | MS.gene73236:intron | 35.0% | |
| ATACAACCCTGTTTACAAGA+AGG | - | chr4.1:78430911-78430930 | MS.gene73236:CDS | 35.0% | |
| ATAGGAACCTTCTTGTAAAC+AGG | + | chr4.1:78430921-78430940 | None:intergenic | 35.0% | |
| ATGATAAGAGACTCAGCAAT+TGG | + | chr4.1:78430963-78430982 | None:intergenic | 35.0% | |
| GCTTCAAAATGCAGTTAACT+TGG | - | chr4.1:78431840-78431859 | MS.gene73236:intron | 35.0% | |
| GTTCCTATTCTTGTTCACAA+TGG | - | chr4.1:78430933-78430952 | MS.gene73236:CDS | 35.0% | |
| TAAGGATCTTGAGACAAAAG+TGG | + | chr4.1:78431020-78431039 | None:intergenic | 35.0% | |
| TAGGAACCTTCTTGTAAACA+GGG | + | chr4.1:78430920-78430939 | None:intergenic | 35.0% | |
| TATCAATCACCCTGTTATCA+AGG | - | chr4.1:78432360-78432379 | MS.gene73236:CDS | 35.0% | |
| TGTCCAACAATTCTAAGTTG+GGG | + | chr4.1:78431772-78431791 | None:intergenic | 35.0% | |
| ! | CTGCCAATTTCATTGGTTAT+TGG | - | chr4.1:78432254-78432273 | MS.gene73236:CDS | 35.0% |
| !! | AGAAGCTCTAAAGATACTAG+AGG | - | chr4.1:78432174-78432193 | MS.gene73236:CDS | 35.0% |
| !! | CTAATTCCTGAGTGTTGATT+TGG | + | chr4.1:78431696-78431715 | None:intergenic | 35.0% |
| !! | TGAAGCTGAAGCTTTGAAAA+AGG | + | chr4.1:78432416-78432435 | None:intergenic | 35.0% |
| AATCACCCTGTTATCAAGGA+AGG | - | chr4.1:78432364-78432383 | MS.gene73236:CDS | 40.0% | |
| AGAATGCACGAGAAACTCAT+AGG | + | chr4.1:78431380-78431399 | None:intergenic | 40.0% | |
| AGATCCTTATGAAAGAGCCT+TGG | - | chr4.1:78431031-78431050 | MS.gene73236:CDS | 40.0% | |
| AGCAATGTCTACAAGTCCTA+AGG | + | chr4.1:78432236-78432255 | None:intergenic | 40.0% | |
| AGCTCATTCAAATTGGCATC+AGG | + | chr4.1:78431902-78431921 | None:intergenic | 40.0% | |
| ATCACCCTGTTATCAAGGAA+GGG | - | chr4.1:78432365-78432384 | MS.gene73236:CDS | 40.0% | |
| CAAGAAGAAGTGAGGCTATT+AGG | - | chr4.1:78430777-78430796 | MS.gene73236:CDS | 40.0% | |
| CTGCAAATCAAGAAGAAGTG+AGG | - | chr4.1:78430769-78430788 | MS.gene73236:CDS | 40.0% | |
| GAATGCACGAGAAACTCATA+GGG | + | chr4.1:78431379-78431398 | None:intergenic | 40.0% | |
| GACATTGCTGCCAATTTCAT+TGG | - | chr4.1:78432247-78432266 | MS.gene73236:CDS | 40.0% | |
| GCAAATAGTTCATCTCTTGG+AGG | + | chr4.1:78432394-78432413 | None:intergenic | 40.0% | |
| GCTCATTCAAATTGGCATCA+GGG | + | chr4.1:78431901-78431920 | None:intergenic | 40.0% | |
| GTAGAACCAAATCAACACTC+AGG | - | chr4.1:78431687-78431706 | MS.gene73236:intron | 40.0% | |
| TACCCCCAACTTAGAATTGT+TGG | - | chr4.1:78431766-78431785 | MS.gene73236:intron | 40.0% | |
| TAGAGGTGAGAGAGTTGTTT+AGG | - | chr4.1:78431155-78431174 | MS.gene73236:intron | 40.0% | |
| TCATAGGGTGGGAAAAGTAA+TGG | + | chr4.1:78431364-78431383 | None:intergenic | 40.0% | |
| TGATTGCAAGAGCTATGTCA+GGG | + | chr4.1:78431738-78431757 | None:intergenic | 40.0% | |
| TGTTGGACAATCATTGGTCA+TGG | - | chr4.1:78431783-78431802 | MS.gene73236:intron | 40.0% | |
| TTCTTTGGTGGAGAAACCTT+AGG | - | chr4.1:78432217-78432236 | MS.gene73236:CDS | 40.0% | |
| ! | GTCCAACAATTCTAAGTTGG+GGG | + | chr4.1:78431771-78431790 | None:intergenic | 40.0% |
| ! | GTCTAAGACTCTTGATGACA+AGG | - | chr4.1:78431064-78431083 | MS.gene73236:CDS | 40.0% |
| ! | TCGTAGGTTGGGAAAAGAAA+CGG | - | chr4.1:78431507-78431526 | MS.gene73236:intron | 40.0% |
| CAGCAAGCATTCCATATTGC+AGG | + | chr4.1:78432112-78432131 | None:intergenic | 45.0% | |
| CTGCAATATGGAATGCTTGC+TGG | - | chr4.1:78432110-78432129 | MS.gene73236:intron | 45.0% | |
| CTTATGAAAGAGCCTTGGCT+CGG | - | chr4.1:78431036-78431055 | MS.gene73236:CDS | 45.0% | |
| GAATCAGACCCGATATCTTG+AGG | + | chr4.1:78431877-78431896 | None:intergenic | 45.0% | |
| GTGATTGCAAGAGCTATGTC+AGG | + | chr4.1:78431739-78431758 | None:intergenic | 45.0% | |
| ! | GCAACCCTTCCTTGATAACA+GGG | + | chr4.1:78432372-78432391 | None:intergenic | 45.0% |
| ! | GTTTCTTTTCCCAACCTACG+AGG | + | chr4.1:78431508-78431527 | None:intergenic | 45.0% |
| ! | TCAGGAATTAGTCTCACTGC+TGG | - | chr4.1:78431705-78431724 | MS.gene73236:intron | 45.0% |
| CGAGCCAAGGCTCTTTCATA+AGG | + | chr4.1:78431038-78431057 | None:intergenic | 50.0% | |
| CTTAGACCAAAACCGAGCCA+AGG | + | chr4.1:78431051-78431070 | None:intergenic | 50.0% | |
| GAAAGGACGTGAGAAAGCTG+TGG | - | chr4.1:78432141-78432160 | MS.gene73236:CDS | 50.0% | |
| GCACGAGAAACTCATAGGGT+GGG | + | chr4.1:78431375-78431394 | None:intergenic | 50.0% | |
| TCAGACCCGATATCTTGAGG+AGG | + | chr4.1:78431874-78431893 | None:intergenic | 50.0% | |
| TGCACGAGAAACTCATAGGG+TGG | + | chr4.1:78431376-78431395 | None:intergenic | 50.0% | |
| TGTGCGCCTCCTCAAGATAT+CGG | - | chr4.1:78431865-78431884 | MS.gene73236:intron | 50.0% | |
| ! | GCTTGCTGGAGTGATGAGAA+AGG | - | chr4.1:78432124-78432143 | MS.gene73236:CDS | 50.0% |
| ! | GGCAACCCTTCCTTGATAAC+AGG | + | chr4.1:78432373-78432392 | None:intergenic | 50.0% |
| !!! | AAAGAGCCTTGGCTCGGTTT+TGG | - | chr4.1:78431042-78431061 | MS.gene73236:CDS | 50.0% |
| AGGACGTGAGAAAGCTGTGG+AGG | - | chr4.1:78432144-78432163 | MS.gene73236:CDS | 55.0% | |
| GCGTGAGAACCTCGTAGGTT+GGG | - | chr4.1:78431496-78431515 | MS.gene73236:intron | 55.0% | |
| GTGCGCCTCCTCAAGATATC+GGG | - | chr4.1:78431866-78431885 | MS.gene73236:intron | 55.0% | |
| AAGGCGCGTGAGAACCTCGT+AGG | - | chr4.1:78431491-78431510 | MS.gene73236:intron | 60.0% | |
| CGCGTGAGAACCTCGTAGGT+TGG | - | chr4.1:78431495-78431514 | MS.gene73236:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 78430762 | 78432441 | 78430762 | ID=MS.gene73236 |
| chr4.1 | mRNA | 78430762 | 78432441 | 78430762 | ID=MS.gene73236.t1;Parent=MS.gene73236 |
| chr4.1 | exon | 78432118 | 78432441 | 78432118 | ID=MS.gene73236.t1.exon1;Parent=MS.gene73236.t1 |
| chr4.1 | CDS | 78432118 | 78432441 | 78432118 | ID=cds.MS.gene73236.t1;Parent=MS.gene73236.t1 |
| chr4.1 | exon | 78430762 | 78431100 | 78430762 | ID=MS.gene73236.t1.exon2;Parent=MS.gene73236.t1 |
| chr4.1 | CDS | 78430762 | 78431100 | 78430762 | ID=cds.MS.gene73236.t1;Parent=MS.gene73236.t1 |
| Gene Sequence |
| Protein sequence |
