AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80025


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80025.t1 MTR_0170s0030 89.362 517 53 2 4 518 31 547 0.0 877
MS.gene80025.t1 MTR_5g053270 36.254 331 189 4 56 367 126 453 2.40e-41 159
MS.gene80025.t1 MTR_1g093850 31.214 346 199 6 56 367 133 473 1.83e-32 133
MS.gene80025.t1 MTR_2g100630 23.098 368 222 13 39 367 113 458 1.58e-14 76.6
MS.gene80025.t1 MTR_2g100630 23.098 368 222 13 39 367 72 417 2.16e-14 76.3
MS.gene80025.t1 MTR_8g014900 22.630 327 233 5 61 367 144 470 2.94e-14 75.9
MS.gene80025.t1 MTR_1g016290 40.426 94 51 1 194 282 207 300 2.51e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80025.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.61e-41 160
MS.gene80025.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.61e-41 160
MS.gene80025.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.61e-41 160
MS.gene80025.t1 AT3G25690 30.617 454 264 9 34 448 115 556 1.61e-41 160
MS.gene80025.t1 AT3G25690 30.617 454 264 9 34 448 117 558 1.94e-41 160
MS.gene80025.t1 AT3G25690 29.973 367 214 8 121 448 53 415 1.37e-30 127
MS.gene80025.t1 AT1G52080 27.157 313 213 6 50 355 133 437 6.08e-16 81.3
MS.gene80025.t1 AT1G52080 27.157 313 213 6 50 355 133 437 6.08e-16 81.3

Find 0 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATTATAAATGAGA+TGG - 16768:3523-3542 MS.gene80025:CDS 10.0%
!!! ATAATTTTAATAATACAAGA+TGG + 16768:3877-3896 MS.gene80025:intergenic 10.0%
!!! CTAAATTTTGTTAAAATAAT+AGG - 16768:4335-4354 MS.gene80025:intron 10.0%
!! AACAAAAAATAACACATAAA+AGG + 16768:3114-3133 MS.gene80025:intergenic 15.0%
!! ATTCTTATGTTCATTAATAT+GGG - 16768:3830-3849 MS.gene80025:CDS 15.0%
!! TACCTTAAATTTATTGTTTA+AGG - 16768:4394-4413 MS.gene80025:intron 15.0%
!! TATTAAAGAAAAAATAGAAG+AGG - 16768:3469-3488 MS.gene80025:CDS 15.0%
!! TATTCTTATGTTCATTAATA+TGG - 16768:3829-3848 MS.gene80025:CDS 15.0%
!!! AAGTAATGTTGATTAATTAT+AGG + 16768:4296-4315 MS.gene80025:intergenic 15.0%
!!! TAAAGACAAATCAATTTTTT+GGG + 16768:3169-3188 MS.gene80025:intergenic 15.0%
!!! TAATAAAAAGCATAATAGAA+AGG - 16768:4637-4656 MS.gene80025:CDS 15.0%
!!! TTAAAGACAAATCAATTTTT+TGG + 16768:3170-3189 MS.gene80025:intergenic 15.0%
!! AATCAACATTACTTAAGTTA+TGG - 16768:4302-4321 MS.gene80025:intron 20.0%
!! AATCCTACAAAAAAAATCAT+TGG - 16768:4245-4264 MS.gene80025:intron 20.0%
!! AGCCTTAAACAATAAATTTA+AGG + 16768:4399-4418 MS.gene80025:intergenic 20.0%
!! ATCCTACAAAAAAAATCATT+GGG - 16768:4246-4265 MS.gene80025:intron 20.0%
!! TGCTTAATAAATTGAAAAGA+TGG - 16768:4852-4871 MS.gene80025:CDS 20.0%
!! TTGTTAAAATAATAGGTAGT+AGG - 16768:4342-4361 MS.gene80025:intron 20.0%
!! TTTCAATTTATTAAGCAAAC+TGG + 16768:4849-4868 MS.gene80025:intergenic 20.0%
!!! AAACAATAAATTTAAGGTAG+AGG + 16768:4393-4412 MS.gene80025:intergenic 20.0%
! AAATTGGTTATTCAGATGAT+AGG - 16768:5299-5318 MS.gene80025:intron 25.0%
! AATACAAAATTACAAGCAGA+GGG - 16768:3295-3314 MS.gene80025:CDS 25.0%
! AATTGAAGGTTGTGAAAATA+TGG - 16768:3238-3257 MS.gene80025:CDS 25.0%
! ACATTCAACACTAATCTATT+GGG - 16768:3954-3973 MS.gene80025:intron 25.0%
! AGAAAAGAGTTTATAAAGAG+TGG + 16768:4108-4127 MS.gene80025:intergenic 25.0%
! ATATAAGAGTTTATCCATTC+GGG + 16768:4008-4027 MS.gene80025:intergenic 25.0%
! ATTCAATAAGTTTCCTTTCA+AGG + 16768:3328-3347 MS.gene80025:intergenic 25.0%
! ATTTGCTCATTTCATCATAT+TGG + 16768:5204-5223 MS.gene80025:intergenic 25.0%
! GAAAGTAAGAAGAATATGAA+TGG - 16768:3545-3564 MS.gene80025:CDS 25.0%
! GATATAAGAGTTTATCCATT+CGG + 16768:4009-4028 MS.gene80025:intergenic 25.0%
! TAATACAAAATTACAAGCAG+AGG - 16768:3294-3313 MS.gene80025:CDS 25.0%
! TATTGAATTGAATAGACTGA+AGG - 16768:3340-3359 MS.gene80025:CDS 25.0%
!! TATGGATTATATCACTGAAA+AGG - 16768:3256-3275 MS.gene80025:CDS 25.0%
!!! TGCTTGATAATTTTTTCTAC+AGG - 16768:4448-4467 MS.gene80025:intron 25.0%
AATACAACCAGAAACTATGT+TGG - 16768:4213-4232 MS.gene80025:intron 30.0%
AATCAAATCTATCAAAGCCA+AGG - 16768:4972-4991 MS.gene80025:CDS 30.0%
ATGCTAAAAGGACTAACAAA+AGG + 16768:5363-5382 MS.gene80025:intergenic 30.0%
ATGTTCATTAATATGGGACT+TGG - 16768:3836-3855 MS.gene80025:CDS 30.0%
ATTGAATTGCCACAAATTGA+AGG - 16768:3224-3243 MS.gene80025:CDS 30.0%
CACATTCAACACTAATCTAT+TGG - 16768:3953-3972 MS.gene80025:intron 30.0%
GAATTCAACTATTACATGCT+TGG - 16768:5153-5172 MS.gene80025:CDS 30.0%
GAGCAAATCATATGAAAATG+TGG - 16768:5216-5235 MS.gene80025:intron 30.0%
GGTTTCTCTAGATTCATAAA+TGG + 16768:5019-5038 MS.gene80025:intergenic 30.0%
TACCATATTAAAAGCAGACT+AGG + 16768:3907-3926 MS.gene80025:intergenic 30.0%
TGATCCTGAAATATGCTAAA+AGG + 16768:5375-5394 MS.gene80025:intergenic 30.0%
TGCTCACAAAACAATCATAT+TGG + 16768:4168-4187 MS.gene80025:intergenic 30.0%
TTATGCTAGTGATGTAATAC+AGG - 16768:3086-3105 MS.gene80025:CDS 30.0%
! AACAAGTTGAAATATTGCAG+AGG - 16768:4537-4556 MS.gene80025:intron 30.0%
! AGATTAGTGTTGAATGTGAA+AGG + 16768:3951-3970 MS.gene80025:intergenic 30.0%
! CACTTATTTCTTCTTGTATC+TGG + 16768:4487-4506 MS.gene80025:intergenic 30.0%
! GATTAGTGTTGAATGTGAAA+GGG + 16768:3950-3969 MS.gene80025:intergenic 30.0%
! TAGTCCTTTTAGCATATTTC+AGG - 16768:5368-5387 MS.gene80025:CDS 30.0%
! TTTTCACAACCTTCAATTTG+TGG + 16768:3236-3255 MS.gene80025:intergenic 30.0%
!! AACCTAGTCTGCTTTTAATA+TGG - 16768:3902-3921 MS.gene80025:CDS 30.0%
!! AGATAGAGCATTACAAGTTT+TGG - 16768:3673-3692 MS.gene80025:CDS 30.0%
!! GATTTTGAGGAAATGGAAAA+TGG - 16768:3057-3076 MS.gene80025:CDS 30.0%
!! TCTGCTTTTAATATGGTACT+TGG - 16768:3909-3928 MS.gene80025:CDS 30.0%
!! TGAACATGATTTTGAGGAAA+TGG - 16768:3050-3069 MS.gene80025:CDS 30.0%
!!! CACCCAATGATTTTTTTTGT+AGG + 16768:4251-4270 MS.gene80025:intergenic 30.0%
!!! TACAAGTTTTGGAAATGAAG+AGG - 16768:3684-3703 MS.gene80025:CDS 30.0%
AACGAGAAGAATTTGCGAAA+GGG - 16768:4893-4912 MS.gene80025:CDS 35.0%
AAGAAACAATTGCTTCCCTT+CGG - 16768:3432-3451 MS.gene80025:CDS 35.0%
AAGAGCTTGAATTGGAAAAG+AGG - 16768:3714-3733 MS.gene80025:CDS 35.0%
ACTACTAGAAGAACTTTCGA+GGG + 16768:4804-4823 MS.gene80025:intergenic 35.0%
ACTAGCAAGAAACAAATCAG+AGG - 16768:3763-3782 MS.gene80025:CDS 35.0%
AGAGCTTGAATTGGAAAAGA+GGG - 16768:3715-3734 MS.gene80025:CDS 35.0%
AGCAATAGCAAGGATCAAAT+TGG - 16768:5283-5302 MS.gene80025:intron 35.0%
AGCATATGAAAAGCCATCTT+TGG + 16768:4595-4614 MS.gene80025:intergenic 35.0%
CAATTCTTTGCCTGAAATCT+TGG + 16768:4681-4700 MS.gene80025:intergenic 35.0%
CTTGGAGTTGAAGAAACAAA+AGG - 16768:5171-5190 MS.gene80025:CDS 35.0%
GAATAAAGGTAGCAATAGCA+AGG - 16768:5273-5292 MS.gene80025:intron 35.0%
GACGAATTAGCGAAGTATAA+CGG - 16768:3611-3630 MS.gene80025:CDS 35.0%
GAGAAACAAAGAGCTTGAAT+TGG - 16768:3706-3725 MS.gene80025:CDS 35.0%
GATTCCTCTTCAGAGAATAA+AGG - 16768:5259-5278 MS.gene80025:intron 35.0%
TGTTATGCAAGAAGAAGTTG+AGG - 16768:5096-5115 MS.gene80025:CDS 35.0%
TTCCCAACTGAAAATGCAAT+TGG - 16768:3379-3398 MS.gene80025:CDS 35.0%
! AGACTCTGAACATGATTTTG+AGG - 16768:3044-3063 MS.gene80025:CDS 35.0%
! CTCCAATTGCATTTTCAGTT+GGG + 16768:3384-3403 MS.gene80025:intergenic 35.0%
! GATGGCTTTTCATATGCTTA+AGG - 16768:4597-4616 MS.gene80025:intron 35.0%
! TCTCCAATTGCATTTTCAGT+TGG + 16768:3385-3404 MS.gene80025:intergenic 35.0%
! TGTTTTGTGAGCAAAGTGAA+AGG - 16768:4175-4194 MS.gene80025:intron 35.0%
!!! AATTTTGGTTTCGAACCGAA+GGG + 16768:3450-3469 MS.gene80025:intergenic 35.0%
!!! TAATTTTGGTTTCGAACCGA+AGG + 16768:3451-3470 MS.gene80025:intergenic 35.0%
AAGAGCTTGTGTACCAAAGA+TGG - 16768:4579-4598 MS.gene80025:intron 40.0%
ACTGGCACATCTAATCTAGT+CGG + 16768:4084-4103 MS.gene80025:intergenic 40.0%
AGAGGGAAATAAGCCTTGAA+AGG - 16768:3312-3331 MS.gene80025:CDS 40.0%
CAACGAGAAGAATTTGCGAA+AGG - 16768:4892-4911 MS.gene80025:CDS 40.0%
CCATGAACCAACATAGTTTC+TGG + 16768:4223-4242 MS.gene80025:intergenic 40.0%
GAATTGGAAAAGAGGGAAGT+AGG - 16768:3722-3741 MS.gene80025:CDS 40.0%
GACACTCTCTTCAATCTTGT+CGG + 16768:5043-5062 MS.gene80025:intergenic 40.0%
GACTACTAGAAGAACTTTCG+AGG + 16768:4805-4824 MS.gene80025:intergenic 40.0%
GAGTTTATAAAGAGTGGCAC+TGG + 16768:4102-4121 MS.gene80025:intergenic 40.0%
TCAACATCTGATCGTTGACA+TGG + 16768:3574-3593 MS.gene80025:intergenic 40.0%
TGATAGGATTAAAAGCCTGC+TGG - 16768:5315-5334 MS.gene80025:CDS 40.0%
TGTCTCTTCGAATGCTACAT+TGG - 16768:4751-4770 MS.gene80025:CDS 40.0%
! ACGTAGGTTTTCAATGTCGA+TGG - 16768:4946-4965 MS.gene80025:CDS 40.0%
! GCTACCTTTATTCTCTGAAG+AGG + 16768:5266-5285 MS.gene80025:intergenic 40.0%
!! CCAGAAACTATGTTGGTTCA+TGG - 16768:4220-4239 MS.gene80025:intron 40.0%
!! GAGAGTGTCTTTCAGTGATT+CGG - 16768:5054-5073 MS.gene80025:CDS 40.0%
!! TCACTTCTTTTGCTTGCTGA+AGG + 16768:3018-3037 MS.gene80025:intergenic 40.0%
AAGCCAAGGAAAAGTTGTGC+TGG - 16768:4986-5005 MS.gene80025:CDS 45.0%
AATCTATTGGGTTTAGCGCG+TGG - 16768:3966-3985 MS.gene80025:intron 45.0%
ACTAGAAGAACTTTCGAGGG+TGG + 16768:4801-4820 MS.gene80025:intergenic 45.0%
ACTCTCTTCAATCTTGTCGG+TGG + 16768:5040-5059 MS.gene80025:intergenic 45.0%
CAAGACTGCACCAAGATTTC+AGG - 16768:4668-4687 MS.gene80025:CDS 45.0%
GAAAACCTACGTATCAGTCC+AGG + 16768:4938-4957 MS.gene80025:intergenic 45.0%
GAGAAGAATTTGCGAAAGGG+TGG - 16768:4896-4915 MS.gene80025:CDS 45.0%
GATTTCTTGGATGACCCGAA+TGG - 16768:3991-4010 MS.gene80025:intron 45.0%
GCAGAGGAACAGATTTGACA+TGG - 16768:4553-4572 MS.gene80025:intron 45.0%
! TTAGCGCGTGGTTGATTTCT+TGG - 16768:3978-3997 MS.gene80025:intron 45.0%
!! GTATAACGGTGCTACTGCTA+TGG - 16768:3625-3644 MS.gene80025:CDS 45.0%
!!! TATTTTTTCTTTAATAATTT+TGG + 16768:3465-3484 MS.gene80025:intergenic 5.0%
CCAAGGAAAAGTTGTGCTGG+TGG - 16768:4989-5008 MS.gene80025:CDS 50.0%
GGAAATAGACCAAGCAGACC+TGG - 16768:4917-4936 MS.gene80025:CDS 50.0%
! CCACCAGCACAACTTTTCCT+TGG + 16768:4992-5011 MS.gene80025:intergenic 50.0%
! CTTGGTGCAGTCTTGTCTTG+TGG + 16768:4663-4682 MS.gene80025:intergenic 50.0%
ATACTAGCAGCTGCACCAGC+AGG + 16768:5333-5352 MS.gene80025:intergenic 55.0%
CGTATCAGTCCAGGTCTGCT+TGG + 16768:4929-4948 MS.gene80025:intergenic 55.0%
GCAGACCTGGACTGATACGT+AGG - 16768:4930-4949 MS.gene80025:CDS 55.0%


Chromosome Type Strat End Strand Name
16768 gene 3012 5399 3012 ID=MS.gene80025
16768 mRNA 3012 5399 3012 ID=MS.gene80025.t1;Parent=MS.gene80025
16768 exon 5304 5399 5304 ID=MS.gene80025.t1.exon1;Parent=MS.gene80025.t1
16768 CDS 5304 5399 5304 ID=cds.MS.gene80025.t1;Parent=MS.gene80025.t1
16768 exon 4627 5211 4627 ID=MS.gene80025.t1.exon2;Parent=MS.gene80025.t1
16768 CDS 4627 5211 4627 ID=cds.MS.gene80025.t1;Parent=MS.gene80025.t1
16768 exon 3012 3941 3012 ID=MS.gene80025.t1.exon3;Parent=MS.gene80025.t1
16768 CDS 3012 3941 3012 ID=cds.MS.gene80025.t1;Parent=MS.gene80025.t1
Gene Sequence

>MS.gene80025

ATGCCTTCAGCAAGCAAAAGAAGTGATAATTCAGACTCTGAACATGATTTTGAGGAAATGGAAAATGGCATTGCTTATGCTAGTGATGTAATACAGGTACCTTTTATGTGTTATTTTTTGTTTTTTTTGTGTGATAAGTTCAGTAGAAATGTATCCCAAAAAATTGATTTGTCTTTAATTTGCTACAGAATGAAGAAAAGAAGAAGATTAAAATTGAATTGCCACAAATTGAAGGTTGTGAAAATATGGATTATATCACTGAAAAGGAGTTGCTGCAGAATTTAATACAAAATTACAAGCAGAGGGAAATAAGCCTTGAAAGGAAACTTATTGAATTGAATAGACTGAAGGAAGAGAAATCAGTCATTTCCCAACTGAAAATGCAATTGGAGAAAAAGAGAGCAAAATTAAAATCTCTTGAAGAAACAATTGCTTCCCTTCGGTTCGAAACCAAAATTATTAAAGAAAAAATAGAAGAGGATATCATGTGTAAAATGCAGTTAGAGATTGCAAATAAAATTATAAATGAGATGGAAAGTAAGAAGAATATGAATGGAAGCCATGTCAACGATCAGATGTTGACGCTTCAACAACGAGTTGACGAATTAGCGAAGTATAACGGTGCTACTGCTATGGTGAATAAGAAACATGAACATGTTGAAGATAGAGCATTACAAGTTTTGGAAATGAAGAGGAGAAACAAAGAGCTTGAATTGGAAAAGAGGGAAGTAGGAACTAAGTTAGCTATTGCACTAGCAAGAAACAAATCAGAGGTAAGAACACTGCATCATAGAAGAAATTGATTTTTTTAGTTCATTATTCTTATGTTCATTAATATGGGACTTGGACTATTCTGAATATACCATCTTGTATTATTAAAATTATGTTTAAACCTAGTCTGCTTTTAATATGGTACTTGGATTTTGTTCGATATACCCTTTCACATTCAACACTAATCTATTGGGTTTAGCGCGTGGTTGATTTCTTGGATGACCCGAATGGATAAACTCTTATATCAAACTAGATTTTTTATATTATATTGTATATAATTCTAACTCTTTGTTACAAACCGACTAGATTAGATGTGCCAGTGCCACTCTTTATAAACTCTTTTCTGACATTATCGATTTTCGATGTGAGATTTGAATTTTTTCCAATATGATTGTTTTGTGAGCAAAGTGAAAGGAGTTTATTATAATACAATACAACCAGAAACTATGTTGGTTCATGGTTAATCCTACAAAAAAAATCATTGGGTGAAAGAACTGTAACTGTTCTCTACCTATAATTAATCAACATTACTTAAGTTATGGATACTTCAAACTAAATTTTGTTAAAATAATAGGTAGTAGGTAATAGTTATTTTGTTCTTTGTTGTCCTCTACCTTAAATTTATTGTTTAAGGCTAGTTTAAGCACTAAGCAATAAGTTTCTTATGCTTGATAATTTTTTCTACAGGAAACAAAAGTGATCCAGATACAAGAAGAAATAAGTGATTTACAACATGTTCATGAACAACTTTCAGAACAAGTTGAAATATTGCAGAGGAACAGATTTGACATGGTACAAGAGCTTGTGTACCAAAGATGGCTTTTCATATGCTTAAGGTATGAAGTTAACAATCATAATAAAAAGCATAATAGAAAGGCCACAAGACAAGACTGCACCAAGATTTCAGGCAAAGAATTGTTGTATGATGAGAAAACTCATTCATTCACATCTGATTCTGCACATGACACTGTCTCTTCGAATGCTACATTGGATGATTTTGATGAAATCGAAACTACCACCCTCGAAAGTTCTTCTAGTAGTCAAAGTAACAACAGTAGCAATTCCAGTTTGCTTAATAAATTGAAAAGATGGAAGAAATCAAAAGATTACAACGAGAAGAATTTGCGAAAGGGTGGAAATAGACCAAGCAGACCTGGACTGATACGTAGGTTTTCAATGTCGATGGTTGAATCAAATCTATCAAAGCCAAGGAAAAGTTGTGCTGGTGGTGATCCATTTATGAATCTAGAGAAACCACCGACAAGATTGAAGAGAGTGTCTTTCAGTGATTCGGTTAAAAAATTGACTTATCATGTTATGCAAGAAGAAGTTGAGGATGTAACTGATGAAAATGAAACTAACTCTCAGCTGAATTCAACTATTACATGCTTGGAGTTGAAGAAACAAAAGGAAGAAGACCAATATGATGAAATGAGCAAATCATATGAAAATGTGGTTTGCAACTATGAAAAAGTTGATTCCTCTTCAGAGAATAAAGGTAGCAATAGCAAGGATCAAATTGGTTATTCAGATGATAGGATTAAAAGCCTGCTGGTGCAGCTGCTAGTATATTTTTTCCTTTTGTTAGTCCTTTTAGCATATTTCAGGATCAAATAA

Protein sequence

>MS.gene80025.t1

MPSASKRSDNSDSEHDFEEMENGIAYASDVIQNEEKKKIKIELPQIEGCENMDYITEKELLQNLIQNYKQREISLERKLIELNRLKEEKSVISQLKMQLEKKRAKLKSLEETIASLRFETKIIKEKIEEDIMCKMQLEIANKIINEMESKKNMNGSHVNDQMLTLQQRVDELAKYNGATAMVNKKHEHVEDRALQVLEMKRRNKELELEKREVGTKLAIALARNKSEETKVIQIQEEISDLQHVHEQLSEQVEILQRNRFDMVQELVYQRWLFICLRYEVNNHNKKHNRKATRQDCTKISGKELLYDEKTHSFTSDSAHDTVSSNATLDDFDEIETTTLESSSSSQSNNSSNSSLLNKLKRWKKSKDYNEKNLRKGGNRPSRPGLIRRFSMSMVESNLSKPRKSCAGGDPFMNLEKPPTRLKRVSFSDSVKKLTYHVMQEEVEDVTDENETNSQLNSTITCLELKKQKEEDQYDEMSKSYENVVCNYEKVDSSSENKGSNSKDQIGYSDDRIKSLLVQLLVYFFLLLVLLAYFRIK