Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84375.t1 | XP_003595099.1 | 97.7 | 86 | 2 | 0 | 2 | 87 | 145 | 230 | 1.70E-41 | 178.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84375.t1 | P60039 | 86.0 | 86 | 12 | 0 | 2 | 87 | 143 | 228 | 2.1e-40 | 166.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84375.t1 | A0A072VPV6 | 97.7 | 86 | 2 | 0 | 2 | 87 | 176 | 261 | 1.2e-41 | 178.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene84375 | 0.831336 | 1.77E-55 | -1.69E-46 |
| MS.gene049343 | MS.gene84375 | 0.800093 | 1.69E-48 | -1.69E-46 |
| MS.gene050026 | MS.gene84375 | 0.814858 | 1.24E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene84375 | 0.804166 | 2.46E-49 | -1.69E-46 |
| MS.gene050561 | MS.gene84375 | 0.800943 | 1.14E-48 | -1.69E-46 |
| MS.gene050799 | MS.gene84375 | 0.822619 | 2.15E-53 | -1.69E-46 |
| MS.gene051247 | MS.gene84375 | 0.806734 | 7.12E-50 | -1.69E-46 |
| MS.gene051594 | MS.gene84375 | 0.814904 | 1.21E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene84375 | 0.810282 | 1.25E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene84375 | 0.803294 | 3.73E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene84375 | 0.814326 | 1.63E-51 | -1.69E-46 |
| MS.gene051957 | MS.gene84375 | 0.803453 | 3.46E-49 | -1.69E-46 |
| MS.gene052208 | MS.gene84375 | 0.800557 | 1.36E-48 | -1.69E-46 |
| MS.gene052519 | MS.gene84375 | 0.822076 | 2.88E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene84375 | 0.828996 | 6.59E-55 | -1.69E-46 |
| MS.gene052584 | MS.gene84375 | 0.830273 | 3.22E-55 | -1.69E-46 |
| MS.gene052773 | MS.gene84375 | 0.818327 | 2.08E-52 | -1.69E-46 |
| MS.gene053221 | MS.gene84375 | 0.819201 | 1.31E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene84375 | 0.811369 | 7.25E-51 | -1.69E-46 |
| MS.gene053883 | MS.gene84375 | 0.817167 | 3.79E-52 | -1.69E-46 |
| MS.gene053974 | MS.gene84375 | 0.821225 | 4.52E-53 | -1.69E-46 |
| MS.gene05415 | MS.gene84375 | 0.844878 | 5.75E-59 | -1.69E-46 |
| MS.gene05423 | MS.gene84375 | 0.844252 | 8.48E-59 | -1.69E-46 |
| MS.gene054291 | MS.gene84375 | -0.826873 | 2.14E-54 | -1.69E-46 |
| MS.gene054651 | MS.gene84375 | 0.8247 | 7.00E-54 | -1.69E-46 |
| MS.gene054991 | MS.gene84375 | 0.826993 | 2.00E-54 | -1.69E-46 |
| MS.gene055228 | MS.gene84375 | 0.806981 | 6.32E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene84375 | 0.825315 | 5.01E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene84375 | 0.8089 | 2.47E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene84375 | 0.820436 | 6.87E-53 | -1.69E-46 |
| MS.gene056174 | MS.gene84375 | 0.805344 | 1.40E-49 | -1.69E-46 |
| MS.gene056272 | MS.gene84375 | 0.815897 | 7.30E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene84375 | 0.803461 | 3.45E-49 | -1.69E-46 |
| MS.gene057021 | MS.gene84375 | 0.801125 | 1.04E-48 | -1.69E-46 |
| MS.gene057026 | MS.gene84375 | 0.823884 | 1.09E-53 | -1.69E-46 |
| MS.gene057060 | MS.gene84375 | 0.801257 | 9.81E-49 | -1.69E-46 |
| MS.gene057414 | MS.gene84375 | 0.824995 | 5.97E-54 | -1.69E-46 |
| MS.gene057444 | MS.gene84375 | 0.805052 | 1.61E-49 | -1.69E-46 |
| MS.gene057598 | MS.gene84375 | 0.807827 | 4.18E-50 | -1.69E-46 |
| MS.gene057921 | MS.gene84375 | 0.803594 | 3.24E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene84375 | 0.808777 | 2.62E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene84375 | 0.839688 | 1.36E-57 | -1.69E-46 |
| MS.gene058829 | MS.gene84375 | 0.807876 | 4.08E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene84375 | 0.803911 | 2.78E-49 | -1.69E-46 |
| MS.gene059460 | MS.gene84375 | 0.803812 | 2.92E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene84375 | 0.80557 | 1.25E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene84375 | 0.836893 | 7.16E-57 | -1.69E-46 |
| MS.gene060365 | MS.gene84375 | 0.802121 | 6.52E-49 | -1.69E-46 |
| MS.gene060373 | MS.gene84375 | 0.806335 | 8.65E-50 | -1.69E-46 |
| MS.gene060521 | MS.gene84375 | 0.824268 | 8.85E-54 | -1.69E-46 |
| MS.gene060572 | MS.gene84375 | 0.802336 | 5.89E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene84375 | -0.823305 | 1.49E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84375.t1 | MTR_2g038250 | 97.674 | 86 | 2 | 0 | 2 | 87 | 145 | 230 | 7.03e-59 | 181 |
| MS.gene84375.t1 | MTR_1g022385 | 97.674 | 86 | 2 | 0 | 2 | 87 | 176 | 261 | 1.66e-58 | 181 |
| MS.gene84375.t1 | MTR_4g008160 | 89.535 | 86 | 9 | 0 | 2 | 87 | 144 | 229 | 2.45e-54 | 169 |
| MS.gene84375.t1 | MTR_1g022375 | 75.641 | 78 | 19 | 0 | 10 | 87 | 75 | 152 | 4.14e-39 | 127 |
| MS.gene84375.t1 | MTR_7g059170 | 48.000 | 100 | 47 | 1 | 2 | 96 | 147 | 246 | 4.68e-32 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84375.t1 | AT2G44120 | 86.047 | 86 | 12 | 0 | 2 | 87 | 143 | 228 | 1.12e-53 | 167 |
| MS.gene84375.t1 | AT2G44120 | 86.047 | 86 | 12 | 0 | 2 | 87 | 148 | 233 | 1.52e-53 | 167 |
| MS.gene84375.t1 | AT2G01250 | 86.047 | 86 | 12 | 0 | 2 | 87 | 143 | 228 | 3.12e-53 | 166 |
| MS.gene84375.t1 | AT3G13580 | 84.884 | 86 | 13 | 0 | 2 | 87 | 145 | 230 | 3.11e-52 | 164 |
| MS.gene84375.t1 | AT3G13580 | 84.884 | 86 | 13 | 0 | 2 | 87 | 145 | 230 | 3.11e-52 | 164 |
| MS.gene84375.t1 | AT3G13580 | 84.884 | 86 | 13 | 0 | 2 | 87 | 145 | 230 | 3.11e-52 | 164 |
| MS.gene84375.t1 | AT3G13580 | 84.884 | 86 | 13 | 0 | 2 | 87 | 145 | 230 | 3.11e-52 | 164 |
| MS.gene84375.t1 | AT3G13580 | 84.884 | 86 | 13 | 0 | 2 | 87 | 145 | 230 | 3.11e-52 | 164 |
| MS.gene84375.t1 | AT3G13580 | 84.884 | 86 | 13 | 0 | 2 | 87 | 145 | 230 | 3.11e-52 | 164 |
| MS.gene84375.t1 | AT3G13580 | 84.884 | 86 | 13 | 0 | 2 | 87 | 145 | 230 | 3.11e-52 | 164 |
| MS.gene84375.t1 | AT3G13580 | 84.884 | 86 | 13 | 0 | 2 | 87 | 145 | 230 | 3.11e-52 | 164 |
| MS.gene84375.t1 | AT3G13580 | 84.884 | 86 | 13 | 0 | 2 | 87 | 145 | 230 | 3.11e-52 | 164 |
| MS.gene84375.t1 | AT1G80750 | 46.535 | 101 | 48 | 2 | 2 | 96 | 147 | 247 | 6.94e-23 | 89.0 |
Find 25 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAAGCTCAAAGCTCCTT+TGG | 0.284641 | 3.1:-4490998 | MS.gene84375:CDS |
| AGTGTTCAATTGCAGGTTCT+TGG | 0.317764 | 3.1:-4491111 | MS.gene84375:intron |
| TTCAAGCTCAAAGCTCCTTT+GGG | 0.329811 | 3.1:-4490997 | MS.gene84375:CDS |
| TTGATCTACAAGCGCGGTTA+TGG | 0.337108 | 3.1:-4491460 | MS.gene84375:CDS |
| GGTGGTGATGCTGGTATAAC+AGG | 0.362244 | 3.1:-4490940 | MS.gene84375:CDS |
| GTGTTCAATTGCAGGTTCTT+GGG | 0.403327 | 3.1:-4491110 | MS.gene84375:intron |
| ACATCAATGAACTCATTAGA+AGG | 0.462677 | 3.1:-4490910 | MS.gene84375:CDS |
| AGATAACTCTATCATTGAAC+AGG | 0.482014 | 3.1:-4491407 | MS.gene84375:intron |
| AAGAGAAATCACTATGTTGA+AGG | 0.489111 | 3.1:-4490961 | MS.gene84375:CDS |
| AAAGGAGCTTTGAGCTTGAA+AGG | 0.504831 | 3.1:+4491000 | None:intergenic |
| GAGAGGCAAACAACTTCCTA+TGG | 0.504947 | 3.1:-4491023 | MS.gene84375:CDS |
| TATGTTGAAGGTGGTGATGC+TGG | 0.512402 | 3.1:-4490949 | MS.gene84375:CDS |
| TTGCAGGTTCTTGGGAAACA+TGG | 0.547799 | 3.1:-4491102 | MS.gene84375:intron |
| ATCCACGAGATCATGACTGT+TGG | 0.549865 | 3.1:-4491057 | MS.gene84375:CDS |
| GCGGTTATGGAAAGGTTGAT+AGG | 0.549876 | 3.1:-4491447 | MS.gene84375:CDS |
| TTCTTCTTCATGCCACCCAA+AGG | 0.554983 | 3.1:+4490982 | None:intergenic |
| CTTTGAGCTTGAAAGGCCAT+AGG | 0.568033 | 3.1:+4491007 | None:intergenic |
| AAGCTCAAAGCTCCTTTGGG+TGG | 0.570830 | 3.1:-4490994 | MS.gene84375:CDS |
| ATCCAACAGTCATGATCTCG+TGG | 0.585385 | 3.1:+4491055 | None:intergenic |
| CTACAAGCGCGGTTATGGAA+AGG | 0.599869 | 3.1:-4491455 | MS.gene84375:CDS |
| AGAAATCACTATGTTGAAGG+TGG | 0.637998 | 3.1:-4490958 | MS.gene84375:CDS |
| GTGGTGATGCTGGTATAACA+GGG | 0.670257 | 3.1:-4490939 | MS.gene84375:CDS |
| AGAGAATTGATCTACAAGCG+CGG | 0.721136 | 3.1:-4491466 | MS.gene84375:CDS |
| TGTTGGATCTCACTTCAGAG+AGG | 0.724978 | 3.1:-4491040 | MS.gene84375:CDS |
| GTGATGCTGGTATAACAGGG+AGG | 0.752839 | 3.1:-4490936 | MS.gene84375:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAAATAGAAAAATTTAAC+CGG | + | chr3.1:4491328-4491347 | None:intergenic | 15.0% |
| AAGAGAAATCACTATGTTGA+AGG | - | chr3.1:4491507-4491526 | MS.gene84375:intron | 30.0% | |
| AATTGAGTAAAGTACAGAGT+CGG | + | chr3.1:4491174-4491193 | None:intergenic | 30.0% | |
| ACATCAATGAACTCATTAGA+AGG | - | chr3.1:4491558-4491577 | MS.gene84375:intron | 30.0% | |
| AGATAACTCTATCATTGAAC+AGG | - | chr3.1:4491061-4491080 | MS.gene84375:CDS | 30.0% | |
| !! | GGTGCTATTATTATTGTTGA+AGG | - | chr3.1:4491294-4491313 | MS.gene84375:intron | 30.0% |
| AACAACTCACATTCTGACTT+TGG | + | chr3.1:4491199-4491218 | None:intergenic | 35.0% | |
| AATAATAGCACCACCATACA+GGG | + | chr3.1:4491286-4491305 | None:intergenic | 35.0% | |
| AGAAATCACTATGTTGAAGG+TGG | - | chr3.1:4491510-4491529 | MS.gene84375:intron | 35.0% | |
| ATTGTTGAAGGTTATGTTGC+CGG | - | chr3.1:4491306-4491325 | MS.gene84375:intron | 35.0% | |
| CTGAACTGTATGACATAGAT+GGG | - | chr3.1:4491135-4491154 | MS.gene84375:intron | 35.0% | |
| TAATAATAGCACCACCATAC+AGG | + | chr3.1:4491287-4491306 | None:intergenic | 35.0% | |
| TCAATTCTCTAACACTCTTC+AGG | + | chr3.1:4490992-4491011 | None:intergenic | 35.0% | |
| TCTGAACTGTATGACATAGA+TGG | - | chr3.1:4491134-4491153 | MS.gene84375:intron | 35.0% | |
| AAGCGTGAAGCTAAGCTTAA+GGG | - | chr3.1:4490950-4490969 | MS.gene84375:CDS | 40.0% | |
| AGAGAATTGATCTACAAGCG+CGG | - | chr3.1:4491002-4491021 | MS.gene84375:CDS | 40.0% | |
| AGTGTTCAATTGCAGGTTCT+TGG | - | chr3.1:4491357-4491376 | MS.gene84375:intron | 40.0% | |
| ATAATAGCACCACCATACAG+GGG | + | chr3.1:4491285-4491304 | None:intergenic | 40.0% | |
| CTGACATAGTGTTCAATTGC+AGG | - | chr3.1:4491350-4491369 | MS.gene84375:intron | 40.0% | |
| GGAATACGATGATTCGCAAA+AGG | - | chr3.1:4490913-4490932 | MS.gene84375:CDS | 40.0% | |
| GTGTTCAATTGCAGGTTCTT+GGG | - | chr3.1:4491358-4491377 | MS.gene84375:intron | 40.0% | |
| TGTGTGATCGAATGAAAGCA+GGG | + | chr3.1:4491106-4491125 | None:intergenic | 40.0% | |
| TTCAAGCTCAAAGCTCCTTT+GGG | - | chr3.1:4491471-4491490 | MS.gene84375:CDS | 40.0% | |
| TTTCAAGCTCAAAGCTCCTT+TGG | - | chr3.1:4491470-4491489 | MS.gene84375:CDS | 40.0% | |
| !! | AAAGGAGCTTTGAGCTTGAA+AGG | + | chr3.1:4491471-4491490 | None:intergenic | 40.0% |
| ATCCAACAGTCATGATCTCG+TGG | + | chr3.1:4491416-4491435 | None:intergenic | 45.0% | |
| ATCCACGAGATCATGACTGT+TGG | - | chr3.1:4491411-4491430 | MS.gene84375:CDS | 45.0% | |
| CTGTGTGATCGAATGAAAGC+AGG | + | chr3.1:4491107-4491126 | None:intergenic | 45.0% | |
| CTTTGAGCTTGAAAGGCCAT+AGG | + | chr3.1:4491464-4491483 | None:intergenic | 45.0% | |
| GAAGCGTGAAGCTAAGCTTA+AGG | - | chr3.1:4490949-4490968 | MS.gene84375:CDS | 45.0% | |
| GAGAGGCAAACAACTTCCTA+TGG | - | chr3.1:4491445-4491464 | MS.gene84375:CDS | 45.0% | |
| TGTTGGATCTCACTTCAGAG+AGG | - | chr3.1:4491428-4491447 | MS.gene84375:CDS | 45.0% | |
| TTCTTCTTCATGCCACCCAA+AGG | + | chr3.1:4491489-4491508 | None:intergenic | 45.0% | |
| TTGATCTACAAGCGCGGTTA+TGG | - | chr3.1:4491008-4491027 | MS.gene84375:CDS | 45.0% | |
| TTGCAGGTTCTTGGGAAACA+TGG | - | chr3.1:4491366-4491385 | MS.gene84375:intron | 45.0% | |
| ! | GCGGTTATGGAAAGGTTGAT+AGG | - | chr3.1:4491021-4491040 | MS.gene84375:CDS | 45.0% |
| ! | GTGGTGATGCTGGTATAACA+GGG | - | chr3.1:4491529-4491548 | MS.gene84375:intron | 45.0% |
| ! | TATGTTGAAGGTGGTGATGC+TGG | - | chr3.1:4491519-4491538 | MS.gene84375:intron | 45.0% |
| AAGCTCAAAGCTCCTTTGGG+TGG | - | chr3.1:4491474-4491493 | MS.gene84375:CDS | 50.0% | |
| CGTGAAGCTAAGCTTAAGGG+TGG | - | chr3.1:4490953-4490972 | MS.gene84375:CDS | 50.0% | |
| CTACAAGCGCGGTTATGGAA+AGG | - | chr3.1:4491013-4491032 | MS.gene84375:CDS | 50.0% | |
| CTTGACCAATGTCCCCTGTA+TGG | - | chr3.1:4491270-4491289 | MS.gene84375:intron | 50.0% | |
| GTGATGCTGGTATAACAGGG+AGG | - | chr3.1:4491532-4491551 | MS.gene84375:intron | 50.0% | |
| ! | GGTGGTGATGCTGGTATAAC+AGG | - | chr3.1:4491528-4491547 | MS.gene84375:intron | 50.0% |
| CACCACCATACAGGGGACAT+TGG | + | chr3.1:4491278-4491297 | None:intergenic | 55.0% | |
| GACCAATGTCCCCTGTATGG+TGG | - | chr3.1:4491273-4491292 | MS.gene84375:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 4490906 | 4491584 | 4490906 | ID=MS.gene84375 |
| chr3.1 | mRNA | 4490906 | 4491584 | 4490906 | ID=MS.gene84375.t1;Parent=MS.gene84375 |
| chr3.1 | exon | 4491582 | 4491584 | 4491582 | ID=MS.gene84375.t1.exon1;Parent=MS.gene84375.t1 |
| chr3.1 | CDS | 4491582 | 4491584 | 4491582 | ID=cds.MS.gene84375.t1;Parent=MS.gene84375.t1 |
| chr3.1 | exon | 4491408 | 4491494 | 4491408 | ID=MS.gene84375.t1.exon2;Parent=MS.gene84375.t1 |
| chr3.1 | CDS | 4491408 | 4491494 | 4491408 | ID=cds.MS.gene84375.t1;Parent=MS.gene84375.t1 |
| chr3.1 | exon | 4490906 | 4491118 | 4490906 | ID=MS.gene84375.t1.exon3;Parent=MS.gene84375.t1 |
| chr3.1 | CDS | 4490906 | 4491118 | 4490906 | ID=cds.MS.gene84375.t1;Parent=MS.gene84375.t1 |
| Gene Sequence |
| Protein sequence |
