Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91221.t1 | XP_003615990.1 | 93.5 | 325 | 17 | 2 | 1 | 321 | 1 | 325 | 1.00E-167 | 599.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91221.t1 | P22195 | 59.5 | 309 | 119 | 4 | 12 | 319 | 11 | 314 | 1.0e-96 | 354.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91221.t1 | Q40372 | 93.5 | 325 | 17 | 2 | 1 | 321 | 1 | 325 | 7.3e-168 | 599.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene91221 | 0.815665 | 8.23E-52 | -1.69E-46 |
| MS.gene049993 | MS.gene91221 | -0.824373 | 8.36E-54 | -1.69E-46 |
| MS.gene050329 | MS.gene91221 | 0.816438 | 5.53E-52 | -1.69E-46 |
| MS.gene050344 | MS.gene91221 | 0.801358 | 9.35E-49 | -1.69E-46 |
| MS.gene050426 | MS.gene91221 | 0.813788 | 2.14E-51 | -1.69E-46 |
| MS.gene051158 | MS.gene91221 | 0.822735 | 2.02E-53 | -1.69E-46 |
| MS.gene051996 | MS.gene91221 | 0.832178 | 1.10E-55 | -1.69E-46 |
| MS.gene052011 | MS.gene91221 | 0.834382 | 3.10E-56 | -1.69E-46 |
| MS.gene052223 | MS.gene91221 | 0.868565 | 5.94E-66 | -1.69E-46 |
| MS.gene05233 | MS.gene91221 | 0.80697 | 6.35E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene91221 | 0.81544 | 9.24E-52 | -1.69E-46 |
| MS.gene052702 | MS.gene91221 | 0.812952 | 3.27E-51 | -1.69E-46 |
| MS.gene053406 | MS.gene91221 | 0.804568 | 2.03E-49 | -1.69E-46 |
| MS.gene054138 | MS.gene91221 | 0.82913 | 6.11E-55 | -1.69E-46 |
| MS.gene054147 | MS.gene91221 | 0.803957 | 2.72E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene91221 | -0.814915 | 1.21E-51 | -1.69E-46 |
| MS.gene054277 | MS.gene91221 | 0.907066 | 7.57E-81 | -1.69E-46 |
| MS.gene054742 | MS.gene91221 | 0.866942 | 1.97E-65 | -1.69E-46 |
| MS.gene055601 | MS.gene91221 | 0.843322 | 1.51E-58 | -1.69E-46 |
| MS.gene056076 | MS.gene91221 | 0.81409 | 1.84E-51 | -1.69E-46 |
| MS.gene056330 | MS.gene91221 | 0.87734 | 6.80E-69 | -1.69E-46 |
| MS.gene056680 | MS.gene91221 | 0.803958 | 2.72E-49 | -1.69E-46 |
| MS.gene056690 | MS.gene91221 | 0.840093 | 1.07E-57 | -1.69E-46 |
| MS.gene056691 | MS.gene91221 | 0.854994 | 8.50E-62 | -1.69E-46 |
| MS.gene056899 | MS.gene91221 | 0.85479 | 9.74E-62 | -1.69E-46 |
| MS.gene056920 | MS.gene91221 | 0.874071 | 8.99E-68 | -1.69E-46 |
| MS.gene056922 | MS.gene91221 | 0.828013 | 1.14E-54 | -1.69E-46 |
| MS.gene056923 | MS.gene91221 | 0.865646 | 5.08E-65 | -1.69E-46 |
| MS.gene056924 | MS.gene91221 | 0.858661 | 7.07E-63 | -1.69E-46 |
| MS.gene056925 | MS.gene91221 | 0.810046 | 1.40E-50 | -1.69E-46 |
| MS.gene057544 | MS.gene91221 | 0.830286 | 3.20E-55 | -1.69E-46 |
| MS.gene058177 | MS.gene91221 | 0.815366 | 9.59E-52 | -1.69E-46 |
| MS.gene058218 | MS.gene91221 | 0.82333 | 1.47E-53 | -1.69E-46 |
| MS.gene059043 | MS.gene91221 | 0.845143 | 4.88E-59 | -1.69E-46 |
| MS.gene059420 | MS.gene91221 | -0.815213 | 1.04E-51 | -1.69E-46 |
| MS.gene060087 | MS.gene91221 | 0.841578 | 4.36E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91221.t1 | MTR_5g074860 | 93.538 | 325 | 17 | 2 | 1 | 321 | 1 | 325 | 0.0 | 615 |
| MS.gene91221.t1 | MTR_5g074860 | 81.846 | 325 | 16 | 3 | 1 | 321 | 1 | 286 | 0.0 | 518 |
| MS.gene91221.t1 | MTR_5g074760 | 70.625 | 320 | 94 | 0 | 1 | 320 | 1 | 320 | 1.05e-169 | 474 |
| MS.gene91221.t1 | MTR_5g074710 | 69.688 | 320 | 97 | 0 | 1 | 320 | 1 | 320 | 5.79e-169 | 472 |
| MS.gene91221.t1 | MTR_5g074740 | 70.000 | 320 | 96 | 0 | 1 | 320 | 1 | 320 | 7.61e-169 | 472 |
| MS.gene91221.t1 | MTR_3g467600 | 66.561 | 314 | 103 | 2 | 9 | 320 | 10 | 323 | 3.32e-152 | 430 |
| MS.gene91221.t1 | MTR_4g029170 | 60.568 | 317 | 119 | 4 | 6 | 320 | 8 | 320 | 5.71e-134 | 384 |
| MS.gene91221.t1 | MTR_3g466200 | 60.252 | 317 | 116 | 7 | 9 | 320 | 8 | 319 | 2.81e-129 | 372 |
| MS.gene91221.t1 | MTR_4g029180 | 62.034 | 295 | 106 | 4 | 28 | 320 | 30 | 320 | 4.10e-128 | 369 |
| MS.gene91221.t1 | MTR_3g094650 | 58.385 | 322 | 126 | 5 | 1 | 319 | 4 | 320 | 1.37e-125 | 362 |
| MS.gene91221.t1 | MTR_5g083860 | 57.895 | 323 | 127 | 6 | 1 | 320 | 8 | 324 | 1.46e-124 | 360 |
| MS.gene91221.t1 | MTR_3g094670 | 59.683 | 315 | 118 | 6 | 9 | 319 | 11 | 320 | 2.02e-121 | 352 |
| MS.gene91221.t1 | MTR_4g029200 | 54.799 | 323 | 138 | 5 | 1 | 320 | 20 | 337 | 6.19e-120 | 348 |
| MS.gene91221.t1 | MTR_5g074970 | 55.927 | 329 | 130 | 7 | 1 | 319 | 1 | 324 | 1.50e-119 | 347 |
| MS.gene91221.t1 | MTR_3g072190 | 54.517 | 321 | 136 | 5 | 5 | 320 | 2 | 317 | 2.56e-117 | 341 |
| MS.gene91221.t1 | MTR_3g094630 | 58.861 | 316 | 121 | 6 | 9 | 320 | 11 | 321 | 2.07e-115 | 336 |
| MS.gene91221.t1 | MTR_2g084010 | 54.574 | 317 | 138 | 5 | 6 | 320 | 5 | 317 | 6.47e-115 | 335 |
| MS.gene91221.t1 | MTR_2g084020 | 53.943 | 317 | 140 | 5 | 6 | 320 | 5 | 317 | 1.71e-114 | 334 |
| MS.gene91221.t1 | MTR_2g084080 | 54.114 | 316 | 138 | 6 | 7 | 320 | 6 | 316 | 1.00e-113 | 332 |
| MS.gene91221.t1 | MTR_1g115900 | 51.077 | 325 | 149 | 6 | 1 | 320 | 1 | 320 | 9.24e-113 | 330 |
| MS.gene91221.t1 | MTR_2g084110 | 53.943 | 317 | 139 | 6 | 6 | 320 | 5 | 316 | 3.54e-112 | 328 |
| MS.gene91221.t1 | MTR_4g029190 | 53.231 | 325 | 142 | 7 | 1 | 320 | 20 | 339 | 7.30e-112 | 328 |
| MS.gene91221.t1 | MTR_3g466180 | 53.822 | 314 | 139 | 4 | 8 | 320 | 14 | 322 | 9.07e-111 | 325 |
| MS.gene91221.t1 | MTR_7g026990 | 51.084 | 323 | 146 | 6 | 5 | 320 | 4 | 321 | 9.17e-111 | 325 |
| MS.gene91221.t1 | MTR_2g084000 | 51.104 | 317 | 148 | 5 | 7 | 320 | 6 | 318 | 4.59e-110 | 323 |
| MS.gene91221.t1 | MTR_7g072480 | 53.226 | 310 | 139 | 4 | 12 | 320 | 11 | 315 | 5.22e-110 | 322 |
| MS.gene91221.t1 | MTR_2g084090 | 51.553 | 322 | 149 | 6 | 1 | 320 | 1 | 317 | 1.22e-109 | 322 |
| MS.gene91221.t1 | MTR_7g072510 | 50.962 | 312 | 147 | 4 | 10 | 320 | 5 | 311 | 2.82e-109 | 320 |
| MS.gene91221.t1 | MTR_4g083710 | 50.153 | 327 | 152 | 7 | 1 | 320 | 1 | 323 | 2.51e-107 | 316 |
| MS.gene91221.t1 | MTR_5g074700 | 67.265 | 223 | 59 | 1 | 98 | 320 | 5 | 213 | 1.46e-104 | 305 |
| MS.gene91221.t1 | MTR_8g076820 | 51.307 | 306 | 137 | 5 | 23 | 320 | 28 | 329 | 2.41e-104 | 308 |
| MS.gene91221.t1 | MTR_5g021060 | 50.492 | 305 | 139 | 5 | 24 | 320 | 29 | 329 | 5.39e-102 | 303 |
| MS.gene91221.t1 | MTR_1g054205 | 47.273 | 330 | 161 | 7 | 1 | 321 | 1 | 326 | 1.08e-99 | 296 |
| MS.gene91221.t1 | MTR_2g029560 | 46.395 | 319 | 160 | 4 | 10 | 320 | 9 | 324 | 1.88e-97 | 291 |
| MS.gene91221.t1 | MTR_4g074995 | 48.254 | 315 | 141 | 7 | 11 | 320 | 10 | 307 | 2.45e-97 | 290 |
| MS.gene91221.t1 | MTR_1g022970 | 49.671 | 304 | 142 | 4 | 25 | 320 | 46 | 346 | 2.08e-96 | 289 |
| MS.gene91221.t1 | MTR_3g072190 | 55.906 | 254 | 106 | 4 | 68 | 320 | 2 | 250 | 7.44e-95 | 281 |
| MS.gene91221.t1 | MTR_2g028980 | 47.962 | 319 | 155 | 4 | 10 | 320 | 14 | 329 | 4.23e-94 | 283 |
| MS.gene91221.t1 | MTR_7g093370 | 46.273 | 322 | 160 | 6 | 8 | 320 | 53 | 370 | 4.40e-94 | 284 |
| MS.gene91221.t1 | MTR_4g122640 | 47.712 | 306 | 150 | 5 | 21 | 320 | 34 | 335 | 5.99e-93 | 280 |
| MS.gene91221.t1 | MTR_4g074990 | 45.794 | 321 | 146 | 7 | 5 | 320 | 7 | 304 | 1.31e-91 | 275 |
| MS.gene91221.t1 | MTR_5g017850 | 48.012 | 327 | 154 | 9 | 4 | 320 | 5 | 325 | 1.37e-91 | 276 |
| MS.gene91221.t1 | MTR_2g040000 | 47.115 | 312 | 149 | 6 | 20 | 320 | 24 | 330 | 5.28e-91 | 275 |
| MS.gene91221.t1 | MTR_2g029750 | 46.875 | 320 | 157 | 6 | 10 | 320 | 14 | 329 | 1.27e-90 | 275 |
| MS.gene91221.t1 | MTR_5g017860 | 46.789 | 327 | 158 | 8 | 4 | 320 | 5 | 325 | 3.30e-90 | 272 |
| MS.gene91221.t1 | MTR_2g029815 | 46.250 | 320 | 161 | 5 | 9 | 320 | 13 | 329 | 6.26e-90 | 273 |
| MS.gene91221.t1 | MTR_4g125940 | 46.319 | 326 | 160 | 6 | 5 | 320 | 8 | 328 | 6.65e-90 | 272 |
| MS.gene91221.t1 | MTR_4g031140 | 47.557 | 307 | 147 | 5 | 21 | 320 | 24 | 323 | 2.49e-89 | 271 |
| MS.gene91221.t1 | MTR_4g074980 | 46.326 | 313 | 139 | 5 | 9 | 320 | 9 | 293 | 1.40e-88 | 267 |
| MS.gene91221.t1 | MTR_2g029800 | 45.455 | 319 | 163 | 4 | 10 | 320 | 14 | 329 | 1.47e-88 | 269 |
| MS.gene91221.t1 | MTR_4g030890 | 47.525 | 303 | 145 | 5 | 25 | 320 | 100 | 395 | 6.91e-88 | 270 |
| MS.gene91221.t1 | MTR_2g029740 | 46.057 | 317 | 158 | 6 | 10 | 317 | 14 | 326 | 8.40e-88 | 267 |
| MS.gene91221.t1 | MTR_5g014100 | 47.419 | 310 | 144 | 7 | 25 | 320 | 26 | 330 | 1.58e-87 | 266 |
| MS.gene91221.t1 | MTR_2g029860 | 43.750 | 320 | 169 | 4 | 10 | 321 | 14 | 330 | 2.75e-87 | 266 |
| MS.gene91221.t1 | MTR_2g029820 | 45.141 | 319 | 164 | 4 | 10 | 320 | 14 | 329 | 5.54e-87 | 265 |
| MS.gene91221.t1 | MTR_2g029830 | 45.130 | 308 | 158 | 4 | 21 | 320 | 24 | 328 | 1.35e-86 | 264 |
| MS.gene91221.t1 | MTR_2g029730 | 45.427 | 328 | 168 | 5 | 1 | 320 | 1 | 325 | 1.63e-86 | 263 |
| MS.gene91221.t1 | MTR_2g029850 | 46.082 | 319 | 161 | 5 | 10 | 320 | 14 | 329 | 8.20e-86 | 262 |
| MS.gene91221.t1 | MTR_5g049280 | 44.164 | 317 | 157 | 8 | 18 | 320 | 26 | 336 | 1.54e-84 | 258 |
| MS.gene91221.t1 | MTR_1g101830 | 43.887 | 319 | 162 | 9 | 10 | 320 | 12 | 321 | 1.35e-83 | 255 |
| MS.gene91221.t1 | MTR_2g029910 | 43.614 | 321 | 169 | 6 | 10 | 321 | 15 | 332 | 4.31e-82 | 253 |
| MS.gene91221.t1 | MTR_4g125940 | 49.819 | 277 | 124 | 6 | 54 | 320 | 2 | 273 | 7.60e-82 | 249 |
| MS.gene91221.t1 | MTR_4g074980 | 50.000 | 248 | 116 | 5 | 9 | 251 | 9 | 253 | 2.51e-80 | 244 |
| MS.gene91221.t1 | MTR_1g025980 | 42.249 | 329 | 171 | 9 | 1 | 321 | 1 | 318 | 4.48e-80 | 246 |
| MS.gene91221.t1 | MTR_1g077000 | 42.761 | 297 | 160 | 7 | 25 | 320 | 39 | 326 | 3.49e-79 | 244 |
| MS.gene91221.t1 | MTR_0155s0040 | 41.176 | 323 | 178 | 9 | 1 | 320 | 1 | 314 | 2.07e-78 | 242 |
| MS.gene91221.t1 | MTR_5g022870 | 42.500 | 320 | 165 | 10 | 9 | 320 | 7 | 315 | 4.04e-78 | 241 |
| MS.gene91221.t1 | MTR_3g092990 | 41.121 | 321 | 175 | 6 | 9 | 320 | 10 | 325 | 6.54e-76 | 236 |
| MS.gene91221.t1 | MTR_1g038680 | 42.244 | 303 | 160 | 7 | 29 | 320 | 33 | 331 | 2.44e-73 | 229 |
| MS.gene91221.t1 | MTR_7g029030 | 41.195 | 318 | 175 | 9 | 8 | 319 | 7 | 318 | 7.36e-73 | 228 |
| MS.gene91221.t1 | MTR_8g075100 | 41.290 | 310 | 163 | 9 | 19 | 320 | 20 | 318 | 1.68e-72 | 227 |
| MS.gene91221.t1 | MTR_2g088770 | 41.176 | 323 | 171 | 9 | 9 | 320 | 10 | 324 | 1.99e-70 | 222 |
| MS.gene91221.t1 | MTR_1g086320 | 37.920 | 327 | 190 | 5 | 4 | 320 | 9 | 332 | 3.19e-69 | 219 |
| MS.gene91221.t1 | MTR_1g086490 | 38.024 | 334 | 187 | 4 | 4 | 320 | 9 | 339 | 4.27e-69 | 219 |
| MS.gene91221.t1 | MTR_2g029830 | 45.349 | 258 | 130 | 4 | 71 | 320 | 6 | 260 | 2.73e-68 | 215 |
| MS.gene91221.t1 | MTR_6g027440 | 38.994 | 318 | 180 | 8 | 7 | 318 | 2 | 311 | 1.31e-67 | 214 |
| MS.gene91221.t1 | MTR_2g008710 | 40.864 | 301 | 156 | 9 | 29 | 317 | 31 | 321 | 1.10e-65 | 209 |
| MS.gene91221.t1 | MTR_4g127670 | 41.667 | 312 | 153 | 11 | 24 | 319 | 23 | 321 | 2.80e-65 | 208 |
| MS.gene91221.t1 | MTR_7g086870 | 38.983 | 295 | 163 | 8 | 29 | 318 | 26 | 308 | 4.68e-65 | 207 |
| MS.gene91221.t1 | MTR_7g107520 | 39.755 | 327 | 173 | 10 | 7 | 320 | 4 | 319 | 9.08e-65 | 207 |
| MS.gene91221.t1 | MTR_1g009750 | 39.820 | 334 | 180 | 11 | 1 | 320 | 1 | 327 | 1.63e-64 | 207 |
| MS.gene91221.t1 | MTR_7g086820 | 37.874 | 301 | 173 | 7 | 23 | 318 | 18 | 309 | 1.65e-64 | 206 |
| MS.gene91221.t1 | MTR_3g092990 | 38.941 | 321 | 171 | 7 | 9 | 320 | 10 | 314 | 2.45e-64 | 206 |
| MS.gene91221.t1 | MTR_4g114210 | 39.564 | 321 | 171 | 10 | 12 | 319 | 8 | 318 | 4.10e-64 | 205 |
| MS.gene91221.t1 | MTR_4g046713 | 37.195 | 328 | 187 | 8 | 1 | 317 | 3 | 322 | 6.44e-64 | 205 |
| MS.gene91221.t1 | MTR_5g016010 | 37.346 | 324 | 184 | 9 | 8 | 317 | 7 | 325 | 1.24e-63 | 204 |
| MS.gene91221.t1 | MTR_2g099175 | 39.414 | 307 | 163 | 9 | 25 | 318 | 36 | 332 | 6.71e-62 | 200 |
| MS.gene91221.t1 | MTR_8g062780 | 36.646 | 322 | 188 | 8 | 6 | 317 | 5 | 320 | 9.03e-61 | 197 |
| MS.gene91221.t1 | MTR_2g077990 | 37.798 | 336 | 163 | 9 | 25 | 320 | 76 | 405 | 1.28e-60 | 199 |
| MS.gene91221.t1 | MTR_4g095450 | 38.365 | 318 | 175 | 10 | 12 | 317 | 12 | 320 | 2.08e-60 | 196 |
| MS.gene91221.t1 | MTR_4g133800 | 39.000 | 300 | 161 | 10 | 30 | 320 | 46 | 332 | 4.40e-60 | 195 |
| MS.gene91221.t1 | MTR_1g090760 | 35.294 | 323 | 190 | 9 | 9 | 317 | 9 | 326 | 5.02e-60 | 195 |
| MS.gene91221.t1 | MTR_3g072210 | 51.872 | 187 | 85 | 4 | 136 | 320 | 54 | 237 | 1.47e-59 | 191 |
| MS.gene91221.t1 | MTR_4g132490 | 34.653 | 303 | 186 | 5 | 25 | 320 | 29 | 326 | 6.61e-59 | 192 |
| MS.gene91221.t1 | MTR_2g078610 | 37.202 | 336 | 165 | 9 | 25 | 320 | 76 | 405 | 6.71e-59 | 194 |
| MS.gene91221.t1 | MTR_2g067440 | 37.126 | 334 | 165 | 9 | 25 | 320 | 60 | 386 | 2.54e-58 | 192 |
| MS.gene91221.t1 | MTR_0286s0010 | 34.675 | 323 | 195 | 8 | 8 | 320 | 17 | 333 | 8.65e-58 | 189 |
| MS.gene91221.t1 | MTR_6g008075 | 34.675 | 323 | 195 | 8 | 8 | 320 | 17 | 333 | 8.65e-58 | 189 |
| MS.gene91221.t1 | MTR_4g087965 | 37.003 | 327 | 186 | 10 | 4 | 317 | 23 | 342 | 1.38e-57 | 189 |
| MS.gene91221.t1 | MTR_2g437770 | 38.245 | 319 | 170 | 10 | 16 | 320 | 35 | 340 | 3.39e-57 | 188 |
| MS.gene91221.t1 | MTR_6g043280 | 37.417 | 302 | 172 | 9 | 25 | 320 | 41 | 331 | 3.96e-56 | 185 |
| MS.gene91221.t1 | MTR_5g074770 | 78.761 | 113 | 19 | 1 | 209 | 321 | 2 | 109 | 7.76e-56 | 177 |
| MS.gene91221.t1 | MTR_6g043240 | 38.361 | 305 | 173 | 10 | 20 | 320 | 37 | 330 | 2.50e-54 | 181 |
| MS.gene91221.t1 | MTR_2g008160 | 37.540 | 309 | 172 | 10 | 21 | 317 | 20 | 319 | 3.35e-54 | 180 |
| MS.gene91221.t1 | MTR_1g009750 | 36.228 | 334 | 175 | 11 | 1 | 320 | 1 | 310 | 4.61e-53 | 176 |
| MS.gene91221.t1 | MTR_6g043460 | 35.455 | 330 | 192 | 10 | 1 | 317 | 3 | 324 | 1.17e-52 | 177 |
| MS.gene91221.t1 | MTR_2g078280 | 36.533 | 323 | 159 | 9 | 25 | 307 | 76 | 392 | 1.22e-52 | 178 |
| MS.gene91221.t1 | MTR_2g067450 | 35.691 | 311 | 173 | 8 | 25 | 320 | 11 | 309 | 2.81e-51 | 172 |
| MS.gene91221.t1 | MTR_1g066710 | 33.770 | 305 | 188 | 7 | 23 | 320 | 25 | 322 | 6.38e-50 | 169 |
| MS.gene91221.t1 | MTR_3g105790 | 34.251 | 327 | 196 | 6 | 1 | 317 | 1 | 318 | 1.05e-49 | 168 |
| MS.gene91221.t1 | MTR_2g008730 | 35.294 | 323 | 162 | 10 | 8 | 317 | 9 | 297 | 2.54e-49 | 166 |
| MS.gene91221.t1 | MTR_1g066380 | 33.010 | 309 | 185 | 8 | 23 | 320 | 25 | 322 | 1.57e-46 | 160 |
| MS.gene91221.t1 | MTR_5g033470 | 32.459 | 305 | 184 | 10 | 29 | 318 | 77 | 374 | 3.55e-46 | 160 |
| MS.gene91221.t1 | MTR_0147s0010 | 32.622 | 328 | 172 | 10 | 22 | 320 | 74 | 381 | 1.38e-44 | 156 |
| MS.gene91221.t1 | MTR_1g066640 | 32.362 | 309 | 187 | 8 | 23 | 320 | 25 | 322 | 2.92e-44 | 154 |
| MS.gene91221.t1 | MTR_7g072490 | 40.650 | 246 | 86 | 6 | 71 | 315 | 2 | 188 | 6.15e-44 | 150 |
| MS.gene91221.t1 | MTR_2g008160 | 34.983 | 303 | 153 | 10 | 21 | 317 | 20 | 284 | 7.72e-44 | 152 |
| MS.gene91221.t1 | MTR_4g132110 | 31.269 | 323 | 206 | 7 | 1 | 317 | 12 | 324 | 8.82e-44 | 153 |
| MS.gene91221.t1 | MTR_1g066680 | 31.922 | 307 | 186 | 9 | 25 | 320 | 27 | 321 | 1.10e-42 | 150 |
| MS.gene91221.t1 | MTR_3g072260 | 57.812 | 128 | 51 | 2 | 8 | 133 | 7 | 133 | 2.80e-42 | 143 |
| MS.gene91221.t1 | MTR_2g029880 | 43.617 | 188 | 84 | 5 | 143 | 320 | 12 | 187 | 8.59e-42 | 144 |
| MS.gene91221.t1 | MTR_4g132110 | 30.793 | 328 | 206 | 8 | 1 | 317 | 12 | 329 | 1.97e-41 | 147 |
| MS.gene91221.t1 | MTR_2g008160 | 45.614 | 171 | 86 | 5 | 29 | 199 | 28 | 191 | 1.52e-39 | 138 |
| MS.gene91221.t1 | MTR_1g066380 | 32.319 | 263 | 156 | 8 | 69 | 320 | 3 | 254 | 1.62e-36 | 132 |
| MS.gene91221.t1 | MTR_3g105790 | 35.417 | 240 | 140 | 5 | 88 | 317 | 8 | 242 | 3.50e-34 | 125 |
| MS.gene91221.t1 | MTR_2g078560 | 32.314 | 229 | 112 | 7 | 131 | 320 | 95 | 319 | 2.52e-25 | 103 |
| MS.gene91221.t1 | MTR_2g077980 | 30.104 | 289 | 151 | 10 | 77 | 320 | 1 | 283 | 8.35e-24 | 99.0 |
| MS.gene91221.t1 | MTR_3g462850 | 39.860 | 143 | 69 | 6 | 192 | 320 | 9 | 148 | 3.34e-22 | 91.3 |
| MS.gene91221.t1 | MTR_4g031040 | 36.310 | 168 | 68 | 3 | 86 | 253 | 3 | 131 | 9.63e-22 | 89.7 |
| MS.gene91221.t1 | MTR_5g058120 | 36.709 | 158 | 89 | 5 | 163 | 317 | 6 | 155 | 9.42e-21 | 90.1 |
| MS.gene91221.t1 | MTR_7g086790 | 34.737 | 190 | 105 | 8 | 72 | 260 | 17 | 188 | 1.46e-19 | 85.5 |
| MS.gene91221.t1 | MTR_2g029930 | 42.056 | 107 | 37 | 2 | 8 | 103 | 2 | 94 | 2.87e-18 | 79.3 |
| MS.gene91221.t1 | MTR_4g114220 | 49.398 | 83 | 31 | 3 | 51 | 133 | 29 | 100 | 5.90e-18 | 79.3 |
| MS.gene91221.t1 | MTR_1g089005 | 37.313 | 134 | 76 | 3 | 11 | 138 | 1 | 132 | 2.88e-17 | 78.2 |
| MS.gene91221.t1 | MTR_6g048160 | 52.055 | 73 | 30 | 3 | 64 | 136 | 22 | 89 | 1.02e-16 | 74.7 |
| MS.gene91221.t1 | MTR_3g107060 | 25.723 | 311 | 160 | 13 | 8 | 302 | 57 | 312 | 5.18e-14 | 72.0 |
| MS.gene91221.t1 | MTR_3g086710 | 51.562 | 64 | 31 | 0 | 7 | 70 | 1 | 64 | 1.38e-13 | 65.9 |
| MS.gene91221.t1 | MTR_4g073410 | 27.444 | 266 | 141 | 9 | 46 | 303 | 22 | 243 | 1.40e-13 | 70.5 |
| MS.gene91221.t1 | MTR_1g098320 | 36.842 | 76 | 46 | 1 | 242 | 317 | 47 | 120 | 4.78e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91221.t1 | AT5G05340 | 58.472 | 301 | 117 | 5 | 23 | 320 | 28 | 323 | 1.58e-122 | 355 |
| MS.gene91221.t1 | AT5G58400 | 53.165 | 316 | 138 | 7 | 9 | 319 | 13 | 323 | 1.43e-107 | 317 |
| MS.gene91221.t1 | AT5G58390 | 53.797 | 316 | 135 | 7 | 10 | 319 | 4 | 314 | 6.97e-106 | 312 |
| MS.gene91221.t1 | AT1G14540 | 50.000 | 316 | 151 | 6 | 8 | 320 | 3 | 314 | 1.65e-105 | 311 |
| MS.gene91221.t1 | AT1G14550 | 49.840 | 313 | 147 | 7 | 11 | 317 | 9 | 317 | 5.83e-102 | 302 |
| MS.gene91221.t1 | AT4G36430 | 49.013 | 304 | 143 | 5 | 25 | 320 | 30 | 329 | 8.13e-101 | 300 |
| MS.gene91221.t1 | AT5G66390 | 48.701 | 308 | 146 | 5 | 21 | 320 | 29 | 332 | 3.69e-100 | 298 |
| MS.gene91221.t1 | AT2G18150 | 48.026 | 304 | 146 | 5 | 25 | 320 | 36 | 335 | 1.18e-99 | 297 |
| MS.gene91221.t1 | AT5G06720 | 46.273 | 322 | 162 | 5 | 8 | 321 | 15 | 333 | 2.89e-98 | 293 |
| MS.gene91221.t1 | AT2G18140 | 47.289 | 332 | 151 | 7 | 9 | 320 | 7 | 334 | 4.10e-98 | 293 |
| MS.gene91221.t1 | AT1G44970 | 49.013 | 304 | 144 | 4 | 25 | 320 | 45 | 345 | 4.99e-95 | 285 |
| MS.gene91221.t1 | AT3G50990 | 48.693 | 306 | 145 | 6 | 23 | 320 | 39 | 340 | 5.63e-95 | 285 |
| MS.gene91221.t1 | AT5G06730 | 45.031 | 322 | 166 | 5 | 8 | 321 | 16 | 334 | 6.18e-94 | 283 |
| MS.gene91221.t1 | AT4G08770 | 46.774 | 310 | 152 | 6 | 21 | 320 | 20 | 326 | 3.33e-89 | 271 |
| MS.gene91221.t1 | AT1G49570 | 47.697 | 304 | 143 | 7 | 25 | 317 | 48 | 346 | 4.16e-89 | 270 |
| MS.gene91221.t1 | AT4G16270 | 48.667 | 300 | 140 | 6 | 29 | 320 | 54 | 347 | 7.99e-88 | 267 |
| MS.gene91221.t1 | AT2G38380 | 46.602 | 309 | 153 | 5 | 21 | 320 | 27 | 332 | 8.89e-88 | 267 |
| MS.gene91221.t1 | AT4G08780 | 45.484 | 310 | 156 | 6 | 21 | 320 | 20 | 326 | 1.36e-87 | 266 |
| MS.gene91221.t1 | AT5G19880 | 44.311 | 334 | 166 | 7 | 1 | 320 | 1 | 328 | 1.07e-86 | 263 |
| MS.gene91221.t1 | AT2G35380 | 48.232 | 311 | 140 | 8 | 25 | 320 | 30 | 334 | 3.75e-86 | 262 |
| MS.gene91221.t1 | AT1G68850 | 41.742 | 333 | 171 | 8 | 8 | 320 | 5 | 334 | 4.51e-86 | 262 |
| MS.gene91221.t1 | AT2G22420 | 42.056 | 321 | 171 | 6 | 7 | 320 | 6 | 318 | 1.25e-85 | 261 |
| MS.gene91221.t1 | AT2G38390 | 46.278 | 309 | 154 | 5 | 21 | 320 | 27 | 332 | 1.66e-85 | 261 |
| MS.gene91221.t1 | AT3G32980 | 45.806 | 310 | 155 | 7 | 21 | 320 | 27 | 333 | 4.87e-85 | 260 |
| MS.gene91221.t1 | AT5G51890 | 43.636 | 330 | 167 | 10 | 1 | 320 | 1 | 321 | 5.52e-85 | 259 |
| MS.gene91221.t1 | AT5G19890 | 46.130 | 323 | 155 | 9 | 8 | 320 | 13 | 326 | 1.25e-84 | 258 |
| MS.gene91221.t1 | AT3G49120 | 45.779 | 308 | 154 | 7 | 23 | 320 | 30 | 334 | 1.85e-83 | 256 |
| MS.gene91221.t1 | AT3G49110 | 44.479 | 326 | 164 | 8 | 9 | 320 | 13 | 335 | 2.79e-83 | 256 |
| MS.gene91221.t1 | AT5G42180 | 43.333 | 330 | 160 | 11 | 5 | 321 | 2 | 317 | 1.88e-82 | 252 |
| MS.gene91221.t1 | AT3G01190 | 43.769 | 329 | 163 | 10 | 1 | 318 | 1 | 318 | 4.23e-78 | 241 |
| MS.gene91221.t1 | AT1G71695 | 43.750 | 304 | 158 | 7 | 20 | 317 | 39 | 335 | 3.17e-75 | 235 |
| MS.gene91221.t1 | AT5G15180 | 41.304 | 322 | 169 | 9 | 10 | 320 | 16 | 328 | 1.12e-73 | 230 |
| MS.gene91221.t1 | AT4G33420 | 41.456 | 316 | 173 | 9 | 8 | 320 | 18 | 324 | 4.29e-73 | 229 |
| MS.gene91221.t1 | AT4G33420 | 41.456 | 316 | 173 | 9 | 8 | 320 | 65 | 371 | 1.46e-72 | 229 |
| MS.gene91221.t1 | AT5G17820 | 39.498 | 319 | 171 | 10 | 9 | 319 | 7 | 311 | 3.22e-68 | 216 |
| MS.gene91221.t1 | AT3G03670 | 38.245 | 319 | 182 | 7 | 9 | 318 | 6 | 318 | 5.63e-68 | 215 |
| MS.gene91221.t1 | AT4G11290 | 38.941 | 321 | 179 | 8 | 8 | 317 | 8 | 322 | 9.91e-68 | 215 |
| MS.gene91221.t1 | AT5G17820 | 39.498 | 319 | 171 | 10 | 9 | 319 | 60 | 364 | 2.79e-67 | 215 |
| MS.gene91221.t1 | AT4G26010 | 39.490 | 314 | 176 | 10 | 8 | 318 | 4 | 306 | 5.49e-67 | 213 |
| MS.gene91221.t1 | AT5G64120 | 41.806 | 299 | 157 | 8 | 29 | 320 | 39 | 327 | 9.07e-67 | 213 |
| MS.gene91221.t1 | AT3G21770 | 40.000 | 315 | 170 | 9 | 14 | 317 | 18 | 324 | 9.65e-67 | 213 |
| MS.gene91221.t1 | AT4G26010 | 39.490 | 314 | 176 | 10 | 8 | 318 | 32 | 334 | 1.06e-66 | 213 |
| MS.gene91221.t1 | AT1G05240 | 38.796 | 299 | 166 | 7 | 29 | 317 | 30 | 321 | 1.23e-66 | 212 |
| MS.gene91221.t1 | AT1G05250 | 38.796 | 299 | 166 | 7 | 29 | 317 | 30 | 321 | 1.23e-66 | 212 |
| MS.gene91221.t1 | AT1G05260 | 37.539 | 317 | 183 | 8 | 10 | 317 | 11 | 321 | 1.29e-66 | 212 |
| MS.gene91221.t1 | AT5G40150 | 37.785 | 307 | 181 | 6 | 16 | 317 | 23 | 324 | 1.08e-64 | 207 |
| MS.gene91221.t1 | AT4G37530 | 39.610 | 308 | 170 | 8 | 21 | 317 | 23 | 325 | 9.50e-64 | 205 |
| MS.gene91221.t1 | AT2G35380 | 47.791 | 249 | 109 | 8 | 87 | 320 | 4 | 246 | 1.20e-63 | 202 |
| MS.gene91221.t1 | AT3G28200 | 37.037 | 297 | 178 | 6 | 25 | 317 | 21 | 312 | 1.70e-62 | 201 |
| MS.gene91221.t1 | AT2G41480 | 37.195 | 328 | 180 | 10 | 9 | 320 | 10 | 327 | 1.55e-61 | 199 |
| MS.gene91221.t1 | AT4G37520 | 38.889 | 306 | 171 | 8 | 23 | 317 | 25 | 325 | 2.52e-61 | 198 |
| MS.gene91221.t1 | AT1G34510 | 37.618 | 319 | 185 | 9 | 1 | 318 | 1 | 306 | 4.72e-61 | 197 |
| MS.gene91221.t1 | AT4G37520 | 38.562 | 306 | 169 | 9 | 23 | 317 | 25 | 322 | 2.40e-59 | 193 |
| MS.gene91221.t1 | AT1G24110 | 35.312 | 320 | 188 | 8 | 8 | 317 | 8 | 318 | 5.50e-59 | 192 |
| MS.gene91221.t1 | AT5G14130 | 35.202 | 321 | 186 | 8 | 9 | 317 | 16 | 326 | 8.37e-59 | 192 |
| MS.gene91221.t1 | AT5G39580 | 38.750 | 320 | 177 | 11 | 10 | 320 | 9 | 318 | 2.50e-58 | 191 |
| MS.gene91221.t1 | AT1G30870 | 37.500 | 312 | 173 | 9 | 18 | 319 | 44 | 343 | 3.15e-58 | 191 |
| MS.gene91221.t1 | AT5G14130 | 35.202 | 321 | 186 | 8 | 9 | 317 | 57 | 367 | 3.24e-58 | 192 |
| MS.gene91221.t1 | AT2G18980 | 36.747 | 332 | 188 | 9 | 1 | 320 | 1 | 322 | 3.49e-58 | 190 |
| MS.gene91221.t1 | AT4G33870 | 33.333 | 342 | 177 | 9 | 23 | 320 | 66 | 400 | 8.49e-58 | 191 |
| MS.gene91221.t1 | AT4G25980 | 36.474 | 329 | 190 | 9 | 1 | 317 | 1 | 322 | 3.31e-57 | 188 |
| MS.gene91221.t1 | AT4G30170 | 37.898 | 314 | 167 | 11 | 22 | 320 | 24 | 324 | 3.51e-57 | 187 |
| MS.gene91221.t1 | AT5G67400 | 37.349 | 332 | 186 | 10 | 1 | 317 | 1 | 325 | 9.02e-57 | 187 |
| MS.gene91221.t1 | AT1G77100 | 39.375 | 320 | 178 | 10 | 8 | 317 | 2 | 315 | 1.82e-56 | 186 |
| MS.gene91221.t1 | AT5G47000 | 34.069 | 317 | 196 | 7 | 8 | 317 | 16 | 326 | 2.51e-56 | 186 |
| MS.gene91221.t1 | AT4G17690 | 38.411 | 302 | 168 | 9 | 25 | 317 | 26 | 318 | 5.78e-56 | 184 |
| MS.gene91221.t1 | AT5G22410 | 35.548 | 301 | 173 | 9 | 29 | 318 | 32 | 322 | 6.90e-56 | 184 |
| MS.gene91221.t1 | AT3G49960 | 36.747 | 332 | 188 | 11 | 1 | 317 | 1 | 325 | 1.27e-55 | 184 |
| MS.gene91221.t1 | AT5G64100 | 39.274 | 303 | 160 | 11 | 27 | 317 | 37 | 327 | 2.77e-55 | 183 |
| MS.gene91221.t1 | AT2G39040 | 38.978 | 313 | 165 | 11 | 25 | 320 | 46 | 349 | 6.08e-55 | 182 |
| MS.gene91221.t1 | AT4G37530 | 40.541 | 259 | 140 | 7 | 21 | 268 | 23 | 278 | 8.94e-54 | 177 |
| MS.gene91221.t1 | AT5G64110 | 39.210 | 329 | 170 | 12 | 11 | 320 | 12 | 329 | 9.12e-54 | 179 |
| MS.gene91221.t1 | AT2G24800 | 35.484 | 310 | 178 | 7 | 23 | 320 | 29 | 328 | 1.38e-52 | 176 |
| MS.gene91221.t1 | AT2G34060 | 36.919 | 344 | 182 | 14 | 3 | 318 | 7 | 343 | 5.18e-52 | 175 |
| MS.gene91221.t1 | AT2G43480 | 35.759 | 316 | 177 | 11 | 20 | 320 | 30 | 334 | 1.84e-51 | 173 |
| MS.gene91221.t1 | AT4G31760 | 32.335 | 334 | 203 | 7 | 1 | 320 | 1 | 325 | 1.26e-50 | 171 |
| MS.gene91221.t1 | AT5G24070 | 35.922 | 309 | 172 | 11 | 25 | 318 | 35 | 332 | 1.13e-49 | 169 |
| MS.gene91221.t1 | AT3G17070 | 31.845 | 336 | 204 | 10 | 3 | 320 | 10 | 338 | 3.69e-47 | 162 |
| MS.gene91221.t1 | AT2G37130 | 32.131 | 305 | 196 | 6 | 22 | 320 | 27 | 326 | 1.48e-44 | 155 |
| MS.gene91221.t1 | AT2G37130 | 32.107 | 299 | 192 | 6 | 28 | 320 | 2 | 295 | 4.86e-44 | 153 |
| MS.gene91221.t1 | AT4G21960 | 32.107 | 299 | 191 | 6 | 25 | 317 | 31 | 323 | 7.16e-44 | 153 |
| MS.gene91221.t1 | AT4G25980 | 40.500 | 200 | 111 | 6 | 1 | 199 | 46 | 238 | 1.73e-37 | 135 |
| MS.gene91221.t1 | AT3G17070 | 33.083 | 266 | 158 | 10 | 67 | 320 | 18 | 275 | 5.30e-35 | 129 |
| MS.gene91221.t1 | AT5G39580 | 40.097 | 207 | 111 | 8 | 10 | 215 | 9 | 203 | 1.86e-34 | 126 |
| MS.gene91221.t1 | AT2G24800 | 41.520 | 171 | 93 | 3 | 23 | 193 | 29 | 192 | 3.70e-32 | 120 |
| MS.gene91221.t1 | AT4G32320 | 26.984 | 315 | 157 | 12 | 5 | 302 | 63 | 321 | 3.09e-15 | 75.9 |
| MS.gene91221.t1 | AT4G35000 | 26.766 | 269 | 139 | 10 | 46 | 303 | 21 | 242 | 1.49e-13 | 70.5 |
| MS.gene91221.t1 | AT3G42570 | 31.469 | 143 | 82 | 5 | 53 | 193 | 19 | 147 | 9.91e-12 | 62.4 |
| MS.gene91221.t1 | AT4G35970 | 31.288 | 163 | 85 | 6 | 46 | 200 | 20 | 163 | 1.63e-11 | 63.5 |
| MS.gene91221.t1 | AT4G35970 | 25.556 | 270 | 140 | 11 | 46 | 303 | 20 | 240 | 2.65e-11 | 63.5 |
Find 56 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTGATCAACAATTGTTTA+AGG | 0.149991 | 5.1:-57530159 | MS.gene91221:CDS |
| CCTCTGATTGAATTAATATT+TGG | 0.153104 | 5.1:+57530859 | None:intergenic |
| AAAGACCTTGTAGCTCTTTC+TGG | 0.185151 | 5.1:-57530389 | MS.gene91221:CDS |
| ATGGAGAACAACTGACTTTA+TGG | 0.215067 | 5.1:+57532016 | None:intergenic |
| TTGTTGTCTCCACCATTTCT+AGG | 0.241506 | 5.1:+57530263 | None:intergenic |
| TTTCTGGTGGTCACACTATA+GGG | 0.295800 | 5.1:-57530373 | MS.gene91221:CDS |
| CTTTCTGGTGGTCACACTAT+AGG | 0.303710 | 5.1:-57530374 | MS.gene91221:CDS |
| GGGAACATCAAGCTTCTTAC+TGG | 0.318572 | 5.1:-57530044 | MS.gene91221:CDS |
| AATATTCATTGTGCTTGTTA+TGG | 0.336901 | 5.1:-57532114 | MS.gene91221:CDS |
| TGTTCTCCATGCAATTCTTA+AGG | 0.346916 | 5.1:-57532003 | MS.gene91221:CDS |
| TCTGATCAACAATTGTTTAA+GGG | 0.362650 | 5.1:-57530158 | MS.gene91221:CDS |
| TAAGACTTCTTTGATCAAGA+TGG | 0.403305 | 5.1:-57530066 | MS.gene91221:CDS |
| CTTAAAGATGCTGCAAAAGT+AGG | 0.404137 | 5.1:+57530296 | None:intergenic |
| ATAGAGATTTGCTATACAAA+AGG | 0.417509 | 5.1:-57530193 | MS.gene91221:CDS |
| AGGTAGTGAAAGTGACAAAT+TGG | 0.427397 | 5.1:-57530132 | MS.gene91221:CDS |
| AGGAAGACTTGTCCTAGAAA+TGG | 0.434931 | 5.1:-57530275 | MS.gene91221:CDS |
| TAGAGATTTGCTATACAAAA+GGG | 0.436085 | 5.1:-57530192 | MS.gene91221:CDS |
| AAGACTTCTTTGATCAAGAT+GGG | 0.453492 | 5.1:-57530065 | MS.gene91221:CDS |
| ACAATTGTTGATCAGAATGA+AGG | 0.465894 | 5.1:+57530165 | None:intergenic |
| TTATGGTTGGTAATGCTTGA+GGG | 0.474257 | 5.1:+57532033 | None:intergenic |
| TTACATTGACGAAGCAATCA+TGG | 0.486125 | 5.1:+57531943 | None:intergenic |
| GGAGTAAGCCATGCGTTGGT+AGG | 0.494219 | 5.1:+57532077 | None:intergenic |
| GATGATACCCCAAATTTCAC+TGG | 0.505975 | 5.1:-57530898 | MS.gene91221:CDS |
| CCAAATATTAATTCAATCAG+AGG | 0.523172 | 5.1:-57530859 | MS.gene91221:CDS |
| AAGTCTCTAGCAAAAGCAGA+AGG | 0.531121 | 5.1:+57530089 | None:intergenic |
| TTAAAGATGCTGCAAAAGTA+GGG | 0.540881 | 5.1:+57530297 | None:intergenic |
| CAACAATTGTTTAAGGGACA+AGG | 0.543449 | 5.1:-57530152 | MS.gene91221:CDS |
| GAGTAAGCCATGCGTTGGTA+GGG | 0.543916 | 5.1:+57532078 | None:intergenic |
| GTCACACTATAGGGTTTGCT+AGG | 0.550006 | 5.1:-57530364 | MS.gene91221:CDS |
| TTTCTCCCCAGTGAAATTTG+GGG | 0.550604 | 5.1:+57530891 | None:intergenic |
| AGCTGTGAGAAGTTGAATGT+TGG | 0.551695 | 5.1:+57530446 | None:intergenic |
| ATTTCAAATTTCAAATCACA+AGG | 0.563348 | 5.1:-57530422 | MS.gene91221:CDS |
| TACAGAATCACGAGCTGCTG+TGG | 0.565085 | 5.1:+57530756 | None:intergenic |
| GAGAAGTTGAATGTTGGAGA+TGG | 0.571079 | 5.1:+57530452 | None:intergenic |
| AGAACAACTGACTTTATGGT+TGG | 0.571386 | 5.1:+57532020 | None:intergenic |
| TTTATGGTTGGTAATGCTTG+AGG | 0.580918 | 5.1:+57532032 | None:intergenic |
| TAGTGAAAGTGACAAATTGG+TGG | 0.589291 | 5.1:-57530129 | MS.gene91221:CDS |
| GACCTTGTAGCTCTTTCTGG+TGG | 0.589326 | 5.1:-57530386 | MS.gene91221:CDS |
| ATATCAGCGCAAGATACCAC+AGG | 0.590740 | 5.1:+57530784 | None:intergenic |
| ATGATACCCCAAATTTCACT+GGG | 0.597994 | 5.1:-57530897 | MS.gene91221:CDS |
| AAGCTTCTTACTGGGAAACA+AGG | 0.598524 | 5.1:-57530035 | MS.gene91221:CDS |
| ATGAGGAGTAAGCCATGCGT+TGG | 0.599175 | 5.1:+57532073 | None:intergenic |
| TATTGATCCCTACCAACGCA+TGG | 0.600690 | 5.1:-57532085 | MS.gene91221:CDS |
| AAGACTTGTCCTAGAAATGG+TGG | 0.602280 | 5.1:-57530272 | MS.gene91221:CDS |
| AGATTCGACTCAATTGCAGA+AGG | 0.610654 | 5.1:-57530010 | MS.gene91221:CDS |
| ACAGTTGATAAAGTATGCAA+AGG | 0.617401 | 5.1:-57530808 | MS.gene91221:CDS |
| CTTGTAGGAGTGAAATCAAG+TGG | 0.621819 | 5.1:+57530233 | None:intergenic |
| AGACTTCTTTGATCAAGATG+GGG | 0.622644 | 5.1:-57530064 | MS.gene91221:CDS |
| GGAACATCAAGCTTCTTACT+GGG | 0.626635 | 5.1:-57530043 | MS.gene91221:CDS |
| AGAGATTTGCTATACAAAAG+GGG | 0.637378 | 5.1:-57530191 | MS.gene91221:CDS |
| AGTCTCTAGCAAAAGCAGAA+GGG | 0.638673 | 5.1:+57530090 | None:intergenic |
| GATTCGACTCAATTGCAGAA+GGG | 0.642604 | 5.1:-57530009 | MS.gene91221:CDS |
| GACCACCAGAAAGAGCTACA+AGG | 0.644551 | 5.1:+57530384 | None:intergenic |
| TAAAGTATGCAAAGGCCCTG+TGG | 0.699533 | 5.1:-57530800 | MS.gene91221:CDS |
| TATCAGCGCAAGATACCACA+GGG | 0.715200 | 5.1:+57530785 | None:intergenic |
| TGATACCCCAAATTTCACTG+GGG | 0.801478 | 5.1:-57530896 | MS.gene91221:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTCAAAATTATTATTATTT+AGG | + | chr5.1:57530389-57530408 | None:intergenic | 10.0% |
| !!! | TTTCTATTTTAATTGATTTT+GGG | - | chr5.1:57530490-57530509 | MS.gene91221:CDS | 10.0% |
| !!! | TTTTCTATTTTAATTGATTT+TGG | - | chr5.1:57530489-57530508 | MS.gene91221:CDS | 10.0% |
| !! | ATTTAAAAATAATGCACAAA+AGG | + | chr5.1:57531422-57531441 | None:intergenic | 15.0% |
| !! | CTAAAATTATGTTTAATCAA+AGG | + | chr5.1:57530452-57530471 | None:intergenic | 15.0% |
| !!! | AATCTTAATTATTTTATGCT+GGG | - | chr5.1:57530807-57530826 | MS.gene91221:CDS | 15.0% |
| !!! | ATATCAATGATTGAAATTTT+AGG | - | chr5.1:57531191-57531210 | MS.gene91221:intron | 15.0% |
| !!! | TATCAATGATTGAAATTTTA+GGG | - | chr5.1:57531192-57531211 | MS.gene91221:intron | 15.0% |
| !! | AAGAAAATTATTGAAGCTAA+CGG | + | chr5.1:57530910-57530929 | None:intergenic | 20.0% |
| !! | ATTTCAAATTTCAAATCACA+AGG | - | chr5.1:57531713-57531732 | MS.gene91221:intron | 20.0% |
| !! | CAAACTTTATCATAAAAATG+AGG | + | chr5.1:57530082-57530101 | None:intergenic | 20.0% |
| !! | TATGTAACACATTAACTTTA+TGG | - | chr5.1:57530301-57530320 | MS.gene91221:CDS | 20.0% |
| !!! | ACTATAACGCTTTAAAATAA+AGG | + | chr5.1:57531522-57531541 | None:intergenic | 20.0% |
| !!! | ACTTCATTTTGAAAATTGTA+TGG | + | chr5.1:57530706-57530725 | None:intergenic | 20.0% |
| !!! | ATGTTTGAAATTGTCAATTT+AGG | + | chr5.1:57530738-57530757 | None:intergenic | 20.0% |
| !!! | GAATCTTAATTATTTTATGC+TGG | - | chr5.1:57530806-57530825 | MS.gene91221:CDS | 20.0% |
| !!! | TACAATTTTCAAAATGAAGT+TGG | - | chr5.1:57530706-57530725 | MS.gene91221:intron | 20.0% |
| !!! | TTTAAAGCGTTATAGTTTTA+GGG | - | chr5.1:57531526-57531545 | MS.gene91221:intron | 20.0% |
| !!! | TTTTAAAGCGTTATAGTTTT+AGG | - | chr5.1:57531525-57531544 | MS.gene91221:intron | 20.0% |
| ! | AACTGTTAAAAGTGACAAAA+TGG | + | chr5.1:57531578-57531597 | None:intergenic | 25.0% |
| ! | ATAATGTTAAGAGATGTTGT+AGG | + | chr5.1:57530270-57530289 | None:intergenic | 25.0% |
| ! | ATGTTGTCTTACCTAATTAT+TGG | - | chr5.1:57530884-57530903 | MS.gene91221:CDS | 25.0% |
| ! | CCAAATATTAATTCAATCAG+AGG | - | chr5.1:57531276-57531295 | MS.gene91221:intron | 25.0% |
| ! | CCTCTGATTGAATTAATATT+TGG | + | chr5.1:57531279-57531298 | None:intergenic | 25.0% |
| ! | TCTGATCAACAATTGTTTAA+GGG | - | chr5.1:57531977-57531996 | MS.gene91221:CDS | 25.0% |
| ! | TTCTGATCAACAATTGTTTA+AGG | - | chr5.1:57531976-57531995 | MS.gene91221:CDS | 25.0% |
| !! | AATATTCATTGTGCTTGTTA+TGG | - | chr5.1:57530021-57530040 | MS.gene91221:CDS | 25.0% |
| !! | ACTTAGACACAATCAATTTT+TGG | + | chr5.1:57531055-57531074 | None:intergenic | 25.0% |
| !! | ATTTTTCTACAATGTGCTAT+TGG | - | chr5.1:57531610-57531629 | MS.gene91221:intron | 25.0% |
| !! | TATGTATTTTCAACTCTTGT+AGG | + | chr5.1:57531920-57531939 | None:intergenic | 25.0% |
| !!! | ATAGAGATTTGCTATACAAA+AGG | - | chr5.1:57531942-57531961 | MS.gene91221:intron | 25.0% |
| !!! | ATTTTGTCACTTTTAACAGT+TGG | - | chr5.1:57531577-57531596 | MS.gene91221:intron | 25.0% |
| !!! | TAGAGATTTGCTATACAAAA+GGG | - | chr5.1:57531943-57531962 | MS.gene91221:intron | 25.0% |
| !!! | TTTTGTCACTTTTAACAGTT+GGG | - | chr5.1:57531578-57531597 | MS.gene91221:intron | 25.0% |
| !!! | TTTTTCTACAATGTGCTATT+GGG | - | chr5.1:57531611-57531630 | MS.gene91221:intron | 25.0% |
| AAAGGATAATAATCACCTCT+AGG | + | chr5.1:57530434-57530453 | None:intergenic | 30.0% | |
| AAGACTTCTTTGATCAAGAT+GGG | - | chr5.1:57532070-57532089 | MS.gene91221:CDS | 30.0% | |
| ACAATTGTTGATCAGAATGA+AGG | + | chr5.1:57531973-57531992 | None:intergenic | 30.0% | |
| ACGAATGTAACTAAATGCAT+TGG | - | chr5.1:57531450-57531469 | MS.gene91221:intron | 30.0% | |
| ACTAAAACCATCTTCAATGT+TGG | - | chr5.1:57530569-57530588 | MS.gene91221:intron | 30.0% | |
| AGCACATTGTAGAAAAATTG+TGG | + | chr5.1:57531608-57531627 | None:intergenic | 30.0% | |
| ATTCTTAAGGAAAAACGCAT+TGG | - | chr5.1:57530145-57530164 | MS.gene91221:CDS | 30.0% | |
| CTTAAAACCAACATTGAAGA+TGG | + | chr5.1:57530579-57530598 | None:intergenic | 30.0% | |
| TAAGACTTCTTTGATCAAGA+TGG | - | chr5.1:57532069-57532088 | MS.gene91221:CDS | 30.0% | |
| TGTGATTTGAATTTGCAATG+TGG | - | chr5.1:57531028-57531047 | MS.gene91221:intron | 30.0% | |
| TTAAAGATGCTGCAAAAGTA+GGG | + | chr5.1:57531841-57531860 | None:intergenic | 30.0% | |
| ! | ATTTTGTGTTTGACATGTGT+CGG | + | chr5.1:57531496-57531515 | None:intergenic | 30.0% |
| ! | GATTTTTCATATCAATGTGC+TGG | - | chr5.1:57530777-57530796 | MS.gene91221:CDS | 30.0% |
| ! | GTTTTTCCTTAAGAATTGCA+TGG | + | chr5.1:57530141-57530160 | None:intergenic | 30.0% |
| ! | TAAATTTTGCTCCACTAGAA+GGG | - | chr5.1:57530627-57530646 | MS.gene91221:intron | 30.0% |
| ! | TTAAATTTTGCTCCACTAGA+AGG | - | chr5.1:57530626-57530645 | MS.gene91221:intron | 30.0% |
| !! | ACACTCTTTAGTTTGAAACA+TGG | + | chr5.1:57530993-57531012 | None:intergenic | 30.0% |
| !! | ACAGTTGATAAAGTATGCAA+AGG | - | chr5.1:57531327-57531346 | MS.gene91221:intron | 30.0% |
| !! | ACTCTTTAGTTTGAAACATG+GGG | + | chr5.1:57530991-57531010 | None:intergenic | 30.0% |
| !! | AGAGATTTGCTATACAAAAG+GGG | - | chr5.1:57531944-57531963 | MS.gene91221:intron | 30.0% |
| !! | CACTCTTTAGTTTGAAACAT+GGG | + | chr5.1:57530992-57531011 | None:intergenic | 30.0% |
| !!! | TTGAAATTTTAGGGTTGTGA+TGG | - | chr5.1:57531201-57531220 | MS.gene91221:intron | 30.0% |
| AAATTGTATGGATGTCCCTT+AGG | + | chr5.1:57530694-57530713 | None:intergenic | 35.0% | |
| AATTGTATGGATGTCCCTTA+GGG | + | chr5.1:57530693-57530712 | None:intergenic | 35.0% | |
| AGACTTCTTTGATCAAGATG+GGG | - | chr5.1:57532071-57532090 | MS.gene91221:CDS | 35.0% | |
| AGGTAGTGAAAGTGACAAAT+TGG | - | chr5.1:57532003-57532022 | MS.gene91221:CDS | 35.0% | |
| ATGATACCCCAAATTTCACT+GGG | - | chr5.1:57531238-57531257 | MS.gene91221:intron | 35.0% | |
| CAACAATTGTTTAAGGGACA+AGG | - | chr5.1:57531983-57532002 | MS.gene91221:CDS | 35.0% | |
| CTTAAAGATGCTGCAAAAGT+AGG | + | chr5.1:57531842-57531861 | None:intergenic | 35.0% | |
| TACATGTTGTAGAAACCTAG+AGG | - | chr5.1:57530416-57530435 | MS.gene91221:CDS | 35.0% | |
| TAGTACCATTTATGCATGCA+TGG | + | chr5.1:57530665-57530684 | None:intergenic | 35.0% | |
| TAGTGAAAGTGACAAATTGG+TGG | - | chr5.1:57532006-57532025 | MS.gene91221:CDS | 35.0% | |
| TGTGCAATTATAAACCGCAA+TGG | + | chr5.1:57530346-57530365 | None:intergenic | 35.0% | |
| TGTTCTCCATGCAATTCTTA+AGG | - | chr5.1:57530132-57530151 | MS.gene91221:CDS | 35.0% | |
| TTACATTGACGAAGCAATCA+TGG | + | chr5.1:57530195-57530214 | None:intergenic | 35.0% | |
| ! | AGAACAACTGACTTTATGGT+TGG | + | chr5.1:57530118-57530137 | None:intergenic | 35.0% |
| ! | ATGGAGAACAACTGACTTTA+TGG | + | chr5.1:57530122-57530141 | None:intergenic | 35.0% |
| ! | CAAAAGGTGTACACTTACTA+TGG | + | chr5.1:57531406-57531425 | None:intergenic | 35.0% |
| ! | TCGATATTTTTCGTCCATTG+CGG | - | chr5.1:57530329-57530348 | MS.gene91221:CDS | 35.0% |
| ! | TTTTCTCCCCAGTGAAATTT+GGG | + | chr5.1:57531248-57531267 | None:intergenic | 35.0% |
| !! | CTACTTTTGCAGCATCTTTA+AGG | - | chr5.1:57531840-57531859 | MS.gene91221:intron | 35.0% |
| !! | TAAATGCATTGGTGTCGTAT+TGG | - | chr5.1:57531461-57531480 | MS.gene91221:intron | 35.0% |
| !! | TTATGGTTGGTAATGCTTGA+GGG | + | chr5.1:57530105-57530124 | None:intergenic | 35.0% |
| !! | TTTATGGTTGGTAATGCTTG+AGG | + | chr5.1:57530106-57530125 | None:intergenic | 35.0% |
| AAAGACCTTGTAGCTCTTTC+TGG | - | chr5.1:57531746-57531765 | MS.gene91221:intron | 40.0% | |
| AAGACTTGTCCTAGAAATGG+TGG | - | chr5.1:57531863-57531882 | MS.gene91221:intron | 40.0% | |
| AAGCTTCTTACTGGGAAACA+AGG | - | chr5.1:57532100-57532119 | MS.gene91221:CDS | 40.0% | |
| AAGTCTCTAGCAAAAGCAGA+AGG | + | chr5.1:57532049-57532068 | None:intergenic | 40.0% | |
| AATGGTACTAGAGTTCCCTA+AGG | - | chr5.1:57530675-57530694 | MS.gene91221:intron | 40.0% | |
| AGATTCGACTCAATTGCAGA+AGG | - | chr5.1:57532125-57532144 | MS.gene91221:CDS | 40.0% | |
| AGCTGTGAGAAGTTGAATGT+TGG | + | chr5.1:57531692-57531711 | None:intergenic | 40.0% | |
| AGGAAGACTTGTCCTAGAAA+TGG | - | chr5.1:57531860-57531879 | MS.gene91221:intron | 40.0% | |
| AGTCTCTAGCAAAAGCAGAA+GGG | + | chr5.1:57532048-57532067 | None:intergenic | 40.0% | |
| ATGGTACTAGAGTTCCCTAA+GGG | - | chr5.1:57530676-57530695 | MS.gene91221:intron | 40.0% | |
| CTTGTAGGAGTGAAATCAAG+TGG | + | chr5.1:57531905-57531924 | None:intergenic | 40.0% | |
| GAAGCTAACGGCCAATAATT+AGG | + | chr5.1:57530898-57530917 | None:intergenic | 40.0% | |
| GAGAAGTTGAATGTTGGAGA+TGG | + | chr5.1:57531686-57531705 | None:intergenic | 40.0% | |
| GATCTCCATGCATGCATAAA+TGG | - | chr5.1:57530657-57530676 | MS.gene91221:intron | 40.0% | |
| GATGATACCCCAAATTTCAC+TGG | - | chr5.1:57531237-57531256 | MS.gene91221:intron | 40.0% | |
| GATTCGACTCAATTGCAGAA+GGG | - | chr5.1:57532126-57532145 | MS.gene91221:CDS | 40.0% | |
| GGAACATCAAGCTTCTTACT+GGG | - | chr5.1:57532092-57532111 | MS.gene91221:CDS | 40.0% | |
| TGATACCCCAAATTTCACTG+GGG | - | chr5.1:57531239-57531258 | MS.gene91221:intron | 40.0% | |
| TTATGCATGCATGGAGATCA+TGG | + | chr5.1:57530656-57530675 | None:intergenic | 40.0% | |
| TTGTTGTCTCCACCATTTCT+AGG | + | chr5.1:57531875-57531894 | None:intergenic | 40.0% | |
| TTTCTCCCCAGTGAAATTTG+GGG | + | chr5.1:57531247-57531266 | None:intergenic | 40.0% | |
| ! | CATATCAATGTGCTGGTGAA+TGG | - | chr5.1:57530784-57530803 | MS.gene91221:CDS | 40.0% |
| ! | GTTTTCTCCCCAGTGAAATT+TGG | + | chr5.1:57531249-57531268 | None:intergenic | 40.0% |
| ! | TTTCTGGTGGTCACACTATA+GGG | - | chr5.1:57531762-57531781 | MS.gene91221:intron | 40.0% |
| !!! | TGTCACTTTTAACAGTTGGG+AGG | - | chr5.1:57531581-57531600 | MS.gene91221:intron | 40.0% |
| ATATCAGCGCAAGATACCAC+AGG | + | chr5.1:57531354-57531373 | None:intergenic | 45.0% | |
| GGGAACATCAAGCTTCTTAC+TGG | - | chr5.1:57532091-57532110 | MS.gene91221:CDS | 45.0% | |
| TAAAGTATGCAAAGGCCCTG+TGG | - | chr5.1:57531335-57531354 | MS.gene91221:intron | 45.0% | |
| TATCAGCGCAAGATACCACA+GGG | + | chr5.1:57531353-57531372 | None:intergenic | 45.0% | |
| TATTGATCCCTACCAACGCA+TGG | - | chr5.1:57530050-57530069 | MS.gene91221:CDS | 45.0% | |
| ! | CTTTCTGGTGGTCACACTAT+AGG | - | chr5.1:57531761-57531780 | MS.gene91221:intron | 45.0% |
| ! | GTCACACTATAGGGTTTGCT+AGG | - | chr5.1:57531771-57531790 | MS.gene91221:intron | 45.0% |
| ATGAGGAGTAAGCCATGCGT+TGG | + | chr5.1:57530065-57530084 | None:intergenic | 50.0% | |
| GACCACCAGAAAGAGCTACA+AGG | + | chr5.1:57531754-57531773 | None:intergenic | 50.0% | |
| GACCTTGTAGCTCTTTCTGG+TGG | - | chr5.1:57531749-57531768 | MS.gene91221:intron | 50.0% | |
| GATCATGGCATCCCTTCTAG+TGG | + | chr5.1:57530641-57530660 | None:intergenic | 50.0% | |
| TACAGAATCACGAGCTGCTG+TGG | + | chr5.1:57531382-57531401 | None:intergenic | 50.0% | |
| ! | GAGTAAGCCATGCGTTGGTA+GGG | + | chr5.1:57530060-57530079 | None:intergenic | 50.0% |
| ! | GGAGTAAGCCATGCGTTGGT+AGG | + | chr5.1:57530061-57530080 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 57530001 | 57532156 | 57530001 | ID=MS.gene91221 |
| chr5.1 | mRNA | 57530001 | 57532156 | 57530001 | ID=MS.gene91221.t1;Parent=MS.gene91221 |
| chr5.1 | exon | 57531947 | 57532156 | 57531947 | ID=MS.gene91221.t1.exon1;Parent=MS.gene91221.t1 |
| chr5.1 | CDS | 57531947 | 57532156 | 57531947 | ID=cds.MS.gene91221.t1;Parent=MS.gene91221.t1 |
| chr5.1 | exon | 57530750 | 57530944 | 57530750 | ID=MS.gene91221.t1.exon2;Parent=MS.gene91221.t1 |
| chr5.1 | CDS | 57530750 | 57530944 | 57530750 | ID=cds.MS.gene91221.t1;Parent=MS.gene91221.t1 |
| chr5.1 | exon | 57530001 | 57530561 | 57530001 | ID=MS.gene91221.t1.exon3;Parent=MS.gene91221.t1 |
| chr5.1 | CDS | 57530001 | 57530561 | 57530001 | ID=cds.MS.gene91221.t1;Parent=MS.gene91221.t1 |
| Gene Sequence |
| Protein sequence |
