Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91914.t1 | XP_003613642.1 | 96.6 | 236 | 2 | 1 | 1 | 230 | 48 | 283 | 6.50E-100 | 373.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91914.t1 | Q8LC03 | 63.5 | 233 | 75 | 3 | 7 | 230 | 63 | 294 | 3.1e-50 | 199.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91914.t1 | G7KB59 | 96.6 | 236 | 2 | 1 | 1 | 230 | 48 | 283 | 4.7e-100 | 373.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene91914.t1 | TF | HB-HD-ZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene91914 | 0.836326 | 9.99E-57 | -1.69E-46 |
| MS.gene049341 | MS.gene91914 | 0.809882 | 1.52E-50 | -1.69E-46 |
| MS.gene049350 | MS.gene91914 | 0.833673 | 4.66E-56 | -1.69E-46 |
| MS.gene049711 | MS.gene91914 | 0.827654 | 1.39E-54 | -1.69E-46 |
| MS.gene049807 | MS.gene91914 | 0.821552 | 3.80E-53 | -1.69E-46 |
| MS.gene049824 | MS.gene91914 | 0.818103 | 2.33E-52 | -1.69E-46 |
| MS.gene049825 | MS.gene91914 | 0.804492 | 2.11E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene91914 | 0.82753 | 1.49E-54 | -1.69E-46 |
| MS.gene05016 | MS.gene91914 | 0.806769 | 7.00E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene91914 | 0.847534 | 1.09E-59 | -1.69E-46 |
| MS.gene050808 | MS.gene91914 | 0.804656 | 1.95E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene91914 | 0.805819 | 1.11E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene91914 | 0.82236 | 2.47E-53 | -1.69E-46 |
| MS.gene051719 | MS.gene91914 | 0.841471 | 4.66E-58 | -1.69E-46 |
| MS.gene051720 | MS.gene91914 | 0.831285 | 1.82E-55 | -1.69E-46 |
| MS.gene052133 | MS.gene91914 | 0.817316 | 3.51E-52 | -1.69E-46 |
| MS.gene052239 | MS.gene91914 | -0.80129 | 9.65E-49 | -1.69E-46 |
| MS.gene052335 | MS.gene91914 | 0.806808 | 6.87E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene91914 | 0.836875 | 7.24E-57 | -1.69E-46 |
| MS.gene053144 | MS.gene91914 | 0.843078 | 1.75E-58 | -1.69E-46 |
| MS.gene053174 | MS.gene91914 | 0.813591 | 2.37E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene91914 | 0.828656 | 7.96E-55 | -1.69E-46 |
| MS.gene053563 | MS.gene91914 | 0.818927 | 1.52E-52 | -1.69E-46 |
| MS.gene053581 | MS.gene91914 | 0.835747 | 1.40E-56 | -1.69E-46 |
| MS.gene053597 | MS.gene91914 | 0.832911 | 7.21E-56 | -1.69E-46 |
| MS.gene053599 | MS.gene91914 | 0.849669 | 2.79E-60 | -1.69E-46 |
| MS.gene05364 | MS.gene91914 | 0.82142 | 4.08E-53 | -1.69E-46 |
| MS.gene054550 | MS.gene91914 | 0.806289 | 8.84E-50 | -1.69E-46 |
| MS.gene054584 | MS.gene91914 | 0.826144 | 3.19E-54 | -1.69E-46 |
| MS.gene054651 | MS.gene91914 | 0.80026 | 1.57E-48 | -1.69E-46 |
| MS.gene055144 | MS.gene91914 | 0.803257 | 3.80E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene91914 | 0.815846 | 7.50E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene91914 | 0.826098 | 3.27E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene91914 | 0.82055 | 6.47E-53 | -1.69E-46 |
| MS.gene055469 | MS.gene91914 | 0.80705 | 6.11E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene91914 | 0.840937 | 6.44E-58 | -1.69E-46 |
| MS.gene055537 | MS.gene91914 | 0.817404 | 3.36E-52 | -1.69E-46 |
| MS.gene055580 | MS.gene91914 | -0.824962 | 6.07E-54 | -1.69E-46 |
| MS.gene055890 | MS.gene91914 | 0.833292 | 5.80E-56 | -1.69E-46 |
| MS.gene055891 | MS.gene91914 | 0.874557 | 6.15E-68 | -1.69E-46 |
| MS.gene056005 | MS.gene91914 | 0.843012 | 1.82E-58 | -1.69E-46 |
| MS.gene056043 | MS.gene91914 | -0.815055 | 1.12E-51 | -1.69E-46 |
| MS.gene056050 | MS.gene91914 | 0.800503 | 1.40E-48 | -1.69E-46 |
| MS.gene056308 | MS.gene91914 | 0.822835 | 1.91E-53 | -1.69E-46 |
| MS.gene056366 | MS.gene91914 | 0.822758 | 2.00E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene91914 | 0.803571 | 3.27E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene91914 | 0.830186 | 3.38E-55 | -1.69E-46 |
| MS.gene058625 | MS.gene91914 | 0.815595 | 8.53E-52 | -1.69E-46 |
| MS.gene058668 | MS.gene91914 | 0.806341 | 8.62E-50 | -1.69E-46 |
| MS.gene058852 | MS.gene91914 | 0.827644 | 1.40E-54 | -1.69E-46 |
| MS.gene05894 | MS.gene91914 | 0.801696 | 7.97E-49 | -1.69E-46 |
| MS.gene059171 | MS.gene91914 | 0.802988 | 4.32E-49 | -1.69E-46 |
| MS.gene059227 | MS.gene91914 | 0.803281 | 3.76E-49 | -1.69E-46 |
| MS.gene060519 | MS.gene91914 | 0.802576 | 5.26E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene91914 | 0.838989 | 2.07E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91914.t1 | MTR_5g039000 | 96.610 | 236 | 2 | 1 | 1 | 230 | 48 | 283 | 4.40e-167 | 462 |
| MS.gene91914.t1 | MTR_8g468210 | 83.966 | 237 | 27 | 5 | 1 | 230 | 50 | 282 | 6.18e-127 | 360 |
| MS.gene91914.t1 | MTR_6g011610 | 54.812 | 239 | 72 | 6 | 1 | 227 | 58 | 272 | 1.17e-74 | 228 |
| MS.gene91914.t1 | MTR_6g011610 | 57.209 | 215 | 65 | 4 | 17 | 227 | 62 | 253 | 2.51e-74 | 226 |
| MS.gene91914.t1 | MTR_7g010020 | 54.148 | 229 | 94 | 7 | 1 | 223 | 48 | 271 | 2.23e-70 | 216 |
| MS.gene91914.t1 | MTR_8g089895 | 44.886 | 176 | 88 | 3 | 18 | 191 | 40 | 208 | 6.43e-39 | 137 |
| MS.gene91914.t1 | MTR_6g007647 | 64.000 | 100 | 36 | 0 | 26 | 125 | 29 | 128 | 1.02e-38 | 135 |
| MS.gene91914.t1 | MTR_6g007647 | 64.000 | 100 | 36 | 0 | 26 | 125 | 63 | 162 | 3.01e-38 | 134 |
| MS.gene91914.t1 | MTR_3g086790 | 57.500 | 120 | 48 | 1 | 17 | 133 | 47 | 166 | 7.02e-38 | 134 |
| MS.gene91914.t1 | MTR_3g080100 | 54.225 | 142 | 54 | 2 | 15 | 145 | 5 | 146 | 7.58e-38 | 131 |
| MS.gene91914.t1 | MTR_3g080100 | 54.225 | 142 | 54 | 2 | 15 | 145 | 44 | 185 | 1.52e-37 | 132 |
| MS.gene91914.t1 | MTR_5g038280 | 60.169 | 118 | 46 | 1 | 8 | 125 | 66 | 182 | 1.63e-37 | 133 |
| MS.gene91914.t1 | MTR_8g469430 | 66.667 | 96 | 32 | 0 | 30 | 125 | 89 | 184 | 7.27e-37 | 130 |
| MS.gene91914.t1 | MTR_4g107650 | 57.025 | 121 | 48 | 1 | 2 | 122 | 71 | 187 | 1.93e-36 | 131 |
| MS.gene91914.t1 | MTR_7g103340 | 50.926 | 108 | 46 | 1 | 18 | 125 | 51 | 151 | 7.23e-31 | 114 |
| MS.gene91914.t1 | MTR_1g061660 | 52.885 | 104 | 46 | 2 | 15 | 117 | 45 | 146 | 4.14e-27 | 104 |
| MS.gene91914.t1 | MTR_3g092150 | 53.846 | 91 | 42 | 0 | 32 | 122 | 40 | 130 | 6.61e-26 | 101 |
| MS.gene91914.t1 | MTR_8g026960 | 41.322 | 121 | 71 | 0 | 4 | 124 | 8 | 128 | 1.11e-23 | 95.5 |
| MS.gene91914.t1 | MTR_4g097600 | 47.573 | 103 | 52 | 1 | 15 | 117 | 114 | 214 | 6.17e-21 | 88.6 |
| MS.gene91914.t1 | MTR_4g100550 | 45.545 | 101 | 54 | 1 | 18 | 117 | 107 | 207 | 7.12e-20 | 85.9 |
| MS.gene91914.t1 | MTR_5g019650 | 47.872 | 94 | 49 | 0 | 30 | 123 | 53 | 146 | 1.69e-19 | 84.0 |
| MS.gene91914.t1 | MTR_5g019680 | 48.889 | 90 | 46 | 0 | 30 | 119 | 53 | 142 | 1.99e-19 | 83.2 |
| MS.gene91914.t1 | MTR_7g093430 | 45.283 | 106 | 54 | 1 | 22 | 127 | 152 | 253 | 7.41e-19 | 83.6 |
| MS.gene91914.t1 | MTR_1g054285 | 46.875 | 96 | 51 | 0 | 22 | 117 | 146 | 241 | 1.09e-18 | 83.2 |
| MS.gene91914.t1 | MTR_5g014890 | 45.000 | 100 | 55 | 0 | 22 | 121 | 115 | 214 | 1.16e-18 | 82.4 |
| MS.gene91914.t1 | MTR_8g006705 | 48.913 | 92 | 47 | 0 | 30 | 121 | 146 | 237 | 1.56e-18 | 82.8 |
| MS.gene91914.t1 | MTR_2g061030 | 41.071 | 112 | 65 | 1 | 6 | 117 | 177 | 287 | 9.17e-18 | 80.9 |
| MS.gene91914.t1 | MTR_1g017090 | 47.727 | 88 | 46 | 0 | 30 | 117 | 130 | 217 | 1.16e-17 | 80.1 |
| MS.gene91914.t1 | MTR_5g013010 | 43.750 | 96 | 54 | 0 | 22 | 117 | 128 | 223 | 8.00e-17 | 77.8 |
| MS.gene91914.t1 | MTR_4g126900 | 44.706 | 85 | 47 | 0 | 33 | 117 | 91 | 175 | 1.91e-16 | 75.5 |
| MS.gene91914.t1 | MTR_4g084750 | 46.591 | 88 | 47 | 0 | 30 | 117 | 65 | 152 | 6.69e-16 | 73.6 |
| MS.gene91914.t1 | MTR_3g103590 | 43.617 | 94 | 53 | 0 | 8 | 101 | 123 | 216 | 7.77e-16 | 75.1 |
| MS.gene91914.t1 | MTR_2g038625 | 38.182 | 110 | 65 | 1 | 11 | 117 | 60 | 169 | 1.04e-13 | 68.2 |
| MS.gene91914.t1 | MTR_2g038580 | 38.182 | 110 | 65 | 1 | 11 | 117 | 60 | 169 | 1.04e-13 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91914.t1 | AT1G69780 | 65.690 | 239 | 72 | 4 | 1 | 230 | 57 | 294 | 2.92e-103 | 301 |
| MS.gene91914.t1 | AT1G26960 | 63.426 | 216 | 59 | 5 | 17 | 230 | 58 | 255 | 7.08e-87 | 258 |
| MS.gene91914.t1 | AT3G01220 | 54.419 | 215 | 81 | 6 | 15 | 223 | 70 | 273 | 6.51e-69 | 213 |
| MS.gene91914.t1 | AT5G15150 | 48.165 | 218 | 85 | 6 | 17 | 223 | 100 | 300 | 8.69e-58 | 186 |
| MS.gene91914.t1 | AT5G65310 | 50.602 | 166 | 75 | 3 | 18 | 176 | 39 | 204 | 8.62e-42 | 144 |
| MS.gene91914.t1 | AT5G65310 | 50.602 | 166 | 75 | 3 | 18 | 176 | 57 | 222 | 1.54e-41 | 144 |
| MS.gene91914.t1 | AT4G40060 | 57.143 | 133 | 52 | 3 | 11 | 139 | 39 | 170 | 5.45e-38 | 134 |
| MS.gene91914.t1 | AT2G22430 | 67.010 | 97 | 32 | 0 | 29 | 125 | 61 | 157 | 1.09e-37 | 134 |
| MS.gene91914.t1 | AT3G01470 | 63.000 | 100 | 37 | 0 | 26 | 125 | 64 | 163 | 6.16e-37 | 130 |
| MS.gene91914.t1 | AT1G27045 | 44.737 | 152 | 77 | 2 | 4 | 148 | 41 | 192 | 3.12e-28 | 107 |
| MS.gene91914.t1 | AT1G27045 | 45.517 | 145 | 72 | 2 | 11 | 148 | 5 | 149 | 5.15e-28 | 105 |
| MS.gene91914.t1 | AT1G27045 | 45.517 | 145 | 72 | 2 | 11 | 148 | 5 | 149 | 5.15e-28 | 105 |
| MS.gene91914.t1 | AT1G27045 | 48.800 | 125 | 58 | 1 | 30 | 148 | 17 | 141 | 2.12e-27 | 103 |
| MS.gene91914.t1 | AT2G46680 | 52.688 | 93 | 44 | 0 | 31 | 123 | 33 | 125 | 1.20e-26 | 103 |
| MS.gene91914.t1 | AT1G27045 | 49.573 | 117 | 58 | 1 | 4 | 119 | 41 | 157 | 1.40e-26 | 103 |
| MS.gene91914.t1 | AT2G46680 | 53.333 | 90 | 42 | 0 | 31 | 120 | 33 | 122 | 7.11e-26 | 101 |
| MS.gene91914.t1 | AT3G61890 | 51.111 | 90 | 44 | 0 | 33 | 122 | 33 | 122 | 2.44e-24 | 97.1 |
| MS.gene91914.t1 | AT5G03790 | 51.136 | 88 | 43 | 0 | 30 | 117 | 77 | 164 | 4.53e-24 | 96.3 |
| MS.gene91914.t1 | AT2G18550 | 40.816 | 147 | 69 | 4 | 20 | 158 | 48 | 184 | 1.86e-22 | 91.7 |
| MS.gene91914.t1 | AT5G03790 | 52.500 | 80 | 38 | 0 | 30 | 109 | 77 | 156 | 1.99e-22 | 90.9 |
| MS.gene91914.t1 | AT4G36740 | 48.544 | 103 | 46 | 1 | 21 | 123 | 47 | 142 | 5.94e-22 | 90.5 |
| MS.gene91914.t1 | AT4G36740 | 48.544 | 103 | 46 | 1 | 21 | 123 | 46 | 141 | 6.45e-22 | 90.1 |
| MS.gene91914.t1 | AT2G36610 | 41.379 | 116 | 58 | 2 | 12 | 119 | 55 | 168 | 1.66e-21 | 88.2 |
| MS.gene91914.t1 | AT5G66700 | 46.552 | 116 | 53 | 2 | 17 | 125 | 60 | 173 | 3.59e-21 | 88.6 |
| MS.gene91914.t1 | AT5G53980 | 45.545 | 101 | 55 | 0 | 25 | 125 | 6 | 106 | 7.24e-21 | 85.9 |
| MS.gene91914.t1 | AT3G60390 | 45.545 | 101 | 55 | 0 | 17 | 117 | 149 | 249 | 4.01e-19 | 84.7 |
| MS.gene91914.t1 | AT4G37790 | 45.000 | 100 | 55 | 0 | 18 | 117 | 114 | 213 | 1.17e-18 | 82.8 |
| MS.gene91914.t1 | AT2G01430 | 44.554 | 101 | 56 | 0 | 17 | 117 | 126 | 226 | 2.75e-18 | 81.6 |
| MS.gene91914.t1 | AT5G47370 | 43.434 | 99 | 56 | 0 | 19 | 117 | 119 | 217 | 3.39e-18 | 81.6 |
| MS.gene91914.t1 | AT5G06710 | 45.263 | 95 | 52 | 0 | 22 | 116 | 182 | 276 | 9.55e-18 | 80.9 |
| MS.gene91914.t1 | AT2G22800 | 44.792 | 96 | 53 | 0 | 22 | 117 | 105 | 200 | 2.94e-17 | 79.0 |
| MS.gene91914.t1 | AT4G17460 | 42.708 | 96 | 55 | 0 | 22 | 117 | 127 | 222 | 9.94e-17 | 77.4 |
| MS.gene91914.t1 | AT2G44910 | 46.591 | 88 | 47 | 0 | 30 | 117 | 163 | 250 | 2.17e-16 | 77.0 |
| MS.gene91914.t1 | AT1G70920 | 37.600 | 125 | 69 | 2 | 9 | 133 | 50 | 165 | 2.91e-15 | 72.0 |
| MS.gene91914.t1 | AT1G70920 | 37.600 | 125 | 69 | 2 | 9 | 133 | 20 | 135 | 3.83e-15 | 71.2 |
| MS.gene91914.t1 | AT4G16780 | 40.625 | 96 | 57 | 0 | 22 | 117 | 121 | 216 | 4.02e-14 | 70.5 |
| MS.gene91914.t1 | AT2G01430 | 51.515 | 66 | 32 | 0 | 17 | 82 | 126 | 191 | 3.02e-13 | 67.0 |
| MS.gene91914.t1 | AT5G06710 | 51.667 | 60 | 29 | 0 | 22 | 81 | 182 | 241 | 1.08e-11 | 63.2 |
Find 33 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGACCATTTGAATTTC+TGG | 0.030649 | 5.3:+27389166 | None:intergenic |
| ATCCTTCTGTTTCTTTGTTC+AGG | 0.195256 | 5.3:+27389359 | None:intergenic |
| CCACCTTCTGCTAGGCCTTC+AGG | 0.235321 | 5.3:-27389223 | MS.gene91914:CDS |
| TGGATGATCAAGCTGGATTT+TGG | 0.257444 | 5.3:-27389114 | MS.gene91914:CDS |
| TTTCTGGCCTTGAAGAAGTT+TGG | 0.289017 | 5.3:+27389182 | None:intergenic |
| GAAGAAGAGGAGGCTTAATA+TGG | 0.330137 | 5.3:-27390478 | MS.gene91914:CDS |
| AACCTAGACAAGTTGCTATA+TGG | 0.331625 | 5.3:-27390365 | MS.gene91914:CDS |
| CTATATGGTTTCAAAATAGA+AGG | 0.336540 | 5.3:-27390350 | MS.gene91914:CDS |
| TCATCTGATAAATCTTCTTC+AGG | 0.406192 | 5.3:+27390519 | None:intergenic |
| GAAAGAGGACTATCACTTGC+TGG | 0.446270 | 5.3:+27389280 | None:intergenic |
| TTCAAAATAGAAGGGCTAGA+TGG | 0.455440 | 5.3:-27390341 | MS.gene91914:CDS |
| CCTTCTTTCCACCTTCTGCT+AGG | 0.463390 | 5.3:-27389231 | MS.gene91914:CDS |
| TTCAGGTTGATTGATTCTGT+TGG | 0.470903 | 5.3:+27389376 | None:intergenic |
| GAAGAAGATTTATCAGATGA+TGG | 0.491350 | 5.3:-27390516 | MS.gene91914:CDS |
| CCTAGCAGAAGGTGGAAAGA+AGG | 0.498274 | 5.3:+27389231 | None:intergenic |
| TTCAAGGCCAGAAATTCAAA+TGG | 0.500653 | 5.3:-27389173 | MS.gene91914:CDS |
| AGGTTCTGCTTGTTGTATGT+TGG | 0.535809 | 5.3:+27389251 | None:intergenic |
| TCAGATGATGGATCACAAGC+TGG | 0.546396 | 5.3:-27390504 | MS.gene91914:CDS |
| CACAAGCTGGAGAGAAGAAG+AGG | 0.557400 | 5.3:-27390491 | MS.gene91914:CDS |
| AGCTGAGCAATTCCACCTGA+AGG | 0.558857 | 5.3:+27389208 | None:intergenic |
| TGTGGTATGGATGATCAAGC+TGG | 0.559995 | 5.3:-27389121 | MS.gene91914:CDS |
| AACCATATAGCAACTTGTCT+AGG | 0.569003 | 5.3:+27390363 | None:intergenic |
| TATATGGTTTCAAAATAGAA+GGG | 0.590755 | 5.3:-27390349 | MS.gene91914:CDS |
| AAATGTTGTTGCTCCAACCA+TGG | 0.592621 | 5.3:+27389091 | None:intergenic |
| CCACCTGAAGGCCTAGCAGA+AGG | 0.595631 | 5.3:+27389220 | None:intergenic |
| CCTGAAGGCCTAGCAGAAGG+TGG | 0.606372 | 5.3:+27389223 | None:intergenic |
| GTAATAAGCTTGAGCCTGAG+AGG | 0.611232 | 5.3:-27390419 | MS.gene91914:CDS |
| AAGCTGGAGAGAAGAAGAGG+AGG | 0.613532 | 5.3:-27390488 | MS.gene91914:CDS |
| GAACAGTTCAGATATCAAGT+TGG | 0.615882 | 5.3:-27389317 | MS.gene91914:CDS |
| TAGATGGAAGACAAAGCAGT+TGG | 0.627956 | 5.3:-27390325 | MS.gene91914:CDS |
| CCTTCTGCTAGGCCTTCAGG+TGG | 0.640058 | 5.3:-27389220 | MS.gene91914:CDS |
| GTATGTTGGTGTGTAGAAAG+AGG | 0.664598 | 5.3:+27389265 | None:intergenic |
| AACCTGAACAAAGAAACAGA+AGG | 0.692102 | 5.3:-27389361 | MS.gene91914:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAGTACAATATATTTAAT+TGG | + | chr5.3:27389682-27389701 | None:intergenic | 10.0% |
| !!! | GTTATTATTTTTTATAACAA+TGG | - | chr5.3:27389734-27389753 | MS.gene91914:intron | 10.0% |
| !! | TTTGTAGAAATAATACTATT+TGG | - | chr5.3:27389812-27389831 | MS.gene91914:intron | 15.0% |
| !!! | AAATTATCTAATTATTGGTT+CGG | + | chr5.3:27390215-27390234 | None:intergenic | 15.0% |
| !!! | ATCTTGATTAATTTACTTTT+AGG | + | chr5.3:27389958-27389977 | None:intergenic | 15.0% |
| !!! | TTTTAATTTGTTACAGATAT+TGG | - | chr5.3:27390232-27390251 | MS.gene91914:intron | 15.0% |
| !! | TATATGGTTTCAAAATAGAA+GGG | - | chr5.3:27389299-27389318 | MS.gene91914:CDS | 20.0% |
| !! | TGAAGTGAAAATAAAATCAT+TGG | + | chr5.3:27389981-27390000 | None:intergenic | 20.0% |
| !!! | ATGAGTATGATTTTGAATTT+CGG | + | chr5.3:27389646-27389665 | None:intergenic | 20.0% |
| !!! | CGGTTAATTTTTTTTTGTAA+CGG | + | chr5.3:27389626-27389645 | None:intergenic | 20.0% |
| !!! | CTATTATATTACAGTTTTAG+AGG | - | chr5.3:27389472-27389491 | MS.gene91914:intron | 20.0% |
| !!! | TCTCTAGAAACTTTATAATA+TGG | + | chr5.3:27390061-27390080 | None:intergenic | 20.0% |
| !!! | TTAATTTACTTTTAGGTGAT+TGG | + | chr5.3:27389951-27389970 | None:intergenic | 20.0% |
| ! | ATGACTTCTTTCTCTTAAAT+AGG | - | chr5.3:27390001-27390020 | MS.gene91914:intron | 25.0% |
| ! | CTATATGGTTTCAAAATAGA+AGG | - | chr5.3:27389298-27389317 | MS.gene91914:CDS | 25.0% |
| !! | TAAGATGAGACATTTTTATG+TGG | + | chr5.3:27390038-27390057 | None:intergenic | 25.0% |
| !!! | TAAAGTTGATGTTAGAGAAT+AGG | + | chr5.3:27389912-27389931 | None:intergenic | 25.0% |
| CAACGTAATTAACTGAAGTT+TGG | - | chr5.3:27389553-27389572 | MS.gene91914:intron | 30.0% | |
| CCTCAAGAACAATTATATTG+AGG | + | chr5.3:27390120-27390139 | None:intergenic | 30.0% | |
| CCTCAATATAATTGTTCTTG+AGG | - | chr5.3:27390117-27390136 | MS.gene91914:intron | 30.0% | |
| GAAGAAGATTTATCAGATGA+TGG | - | chr5.3:27389132-27389151 | MS.gene91914:CDS | 30.0% | |
| GTCTTTACATATGACAAACA+AGG | + | chr5.3:27389583-27389602 | None:intergenic | 30.0% | |
| GTTTGTCATATGTAAAGACT+TGG | - | chr5.3:27389584-27389603 | MS.gene91914:intron | 30.0% | |
| TCATCTGATAAATCTTCTTC+AGG | + | chr5.3:27389132-27389151 | None:intergenic | 30.0% | |
| TCTTTGACCATTTGAATTTC+TGG | + | chr5.3:27390485-27390504 | None:intergenic | 30.0% | |
| ! | AGTTTAAGCAACATGTTTTG+TGG | - | chr5.3:27390509-27390528 | MS.gene91914:CDS | 30.0% |
| !!! | AGTTTTAGAGGTTTATGCAA+CGG | - | chr5.3:27389484-27389503 | MS.gene91914:intron | 30.0% |
| AACCATATAGCAACTTGTCT+AGG | + | chr5.3:27389288-27389307 | None:intergenic | 35.0% | |
| AACCTAGACAAGTTGCTATA+TGG | - | chr5.3:27389283-27389302 | MS.gene91914:CDS | 35.0% | |
| AACCTGAACAAAGAAACAGA+AGG | - | chr5.3:27390287-27390306 | MS.gene91914:CDS | 35.0% | |
| GAACAGTTCAGATATCAAGT+TGG | - | chr5.3:27390331-27390350 | MS.gene91914:CDS | 35.0% | |
| GATGTTAGAGAATAGGTGAA+AGG | + | chr5.3:27389905-27389924 | None:intergenic | 35.0% | |
| TAGAGAATAGGTGAAAGGAA+AGG | + | chr5.3:27389900-27389919 | None:intergenic | 35.0% | |
| TTCAAAATAGAAGGGCTAGA+TGG | - | chr5.3:27389307-27389326 | MS.gene91914:CDS | 35.0% | |
| TTCAAGGCCAGAAATTCAAA+TGG | - | chr5.3:27390475-27390494 | MS.gene91914:CDS | 35.0% | |
| ! | AAACCTCAGCTTGAAGTTTT+TGG | + | chr5.3:27389422-27389441 | None:intergenic | 35.0% |
| ! | AAGCAACATGTTTTGTGGTA+TGG | - | chr5.3:27390514-27390533 | MS.gene91914:CDS | 35.0% |
| ! | AGCTTTTCCAAACTTCTTCA+AGG | - | chr5.3:27390459-27390478 | MS.gene91914:CDS | 35.0% |
| ! | ATCCTTCTGTTTCTTTGTTC+AGG | + | chr5.3:27390292-27390311 | None:intergenic | 35.0% |
| ! | TTTTCATCAGGGATTGAACT+TGG | - | chr5.3:27389090-27389109 | MS.gene91914:CDS | 35.0% |
| !! | TTCAGGTTGATTGATTCTGT+TGG | + | chr5.3:27390275-27390294 | None:intergenic | 35.0% |
| !!! | CTTGAGAAGAGTTTTGAACT+TGG | - | chr5.3:27389207-27389226 | MS.gene91914:CDS | 35.0% |
| AAATGTTGTTGCTCCAACCA+TGG | + | chr5.3:27390560-27390579 | None:intergenic | 40.0% | |
| GAACCAAAAACTTCAAGCTG+AGG | - | chr5.3:27389416-27389435 | MS.gene91914:intron | 40.0% | |
| GAAGAAGAGGAGGCTTAATA+TGG | - | chr5.3:27389170-27389189 | MS.gene91914:CDS | 40.0% | |
| GTATGTTGGTGTGTAGAAAG+AGG | + | chr5.3:27390386-27390405 | None:intergenic | 40.0% | |
| TAGATGGAAGACAAAGCAGT+TGG | - | chr5.3:27389323-27389342 | MS.gene91914:CDS | 40.0% | |
| ! | TGGATGATCAAGCTGGATTT+TGG | - | chr5.3:27390534-27390553 | MS.gene91914:CDS | 40.0% |
| !! | AGGTTCTGCTTGTTGTATGT+TGG | + | chr5.3:27390400-27390419 | None:intergenic | 40.0% |
| !! | TTGGTTCGGTTCTAATTTCG+TGG | + | chr5.3:27390201-27390220 | None:intergenic | 40.0% |
| !! | TTTCTGGCCTTGAAGAAGTT+TGG | + | chr5.3:27390469-27390488 | None:intergenic | 40.0% |
| GAAAGAGGACTATCACTTGC+TGG | + | chr5.3:27390371-27390390 | None:intergenic | 45.0% | |
| GTAATAAGCTTGAGCCTGAG+AGG | - | chr5.3:27389229-27389248 | MS.gene91914:CDS | 45.0% | |
| TGTGGTATGGATGATCAAGC+TGG | - | chr5.3:27390527-27390546 | MS.gene91914:CDS | 45.0% | |
| !! | ATCAAGCTGGATTTTGGCCA+TGG | - | chr5.3:27390540-27390559 | MS.gene91914:CDS | 45.0% |
| !! | TCAGATGATGGATCACAAGC+TGG | - | chr5.3:27389144-27389163 | MS.gene91914:CDS | 45.0% |
| !! | AATTAAAATTATCTAATTAT+TGG | + | chr5.3:27390220-27390239 | None:intergenic | 5.0% |
| !!! | TTATTATTTTTTATAACAAT+GGG | - | chr5.3:27389735-27389754 | MS.gene91914:intron | 5.0% |
| AAGCTGGAGAGAAGAAGAGG+AGG | - | chr5.3:27389160-27389179 | MS.gene91914:CDS | 50.0% | |
| AGCTGAGCAATTCCACCTGA+AGG | + | chr5.3:27390443-27390462 | None:intergenic | 50.0% | |
| CACAAGCTGGAGAGAAGAAG+AGG | - | chr5.3:27389157-27389176 | MS.gene91914:CDS | 50.0% | |
| CCTAGCAGAAGGTGGAAAGA+AGG | + | chr5.3:27390420-27390439 | None:intergenic | 50.0% | |
| CCTTCTTTCCACCTTCTGCT+AGG | - | chr5.3:27390417-27390436 | MS.gene91914:CDS | 50.0% | |
| ! | GCAAGCTGCATTTTCCTCTC+AGG | + | chr5.3:27389246-27389265 | None:intergenic | 50.0% |
| !! | AGCTGGATTTTGGCCATGGT+TGG | - | chr5.3:27390544-27390563 | MS.gene91914:CDS | 50.0% |
| TCGTGGCAAGAAACTGCGCA+TGG | + | chr5.3:27390184-27390203 | None:intergenic | 55.0% | |
| CCACCTGAAGGCCTAGCAGA+AGG | + | chr5.3:27390431-27390450 | None:intergenic | 60.0% | |
| CCACCTTCTGCTAGGCCTTC+AGG | - | chr5.3:27390425-27390444 | MS.gene91914:CDS | 60.0% | |
| CCTGAAGGCCTAGCAGAAGG+TGG | + | chr5.3:27390428-27390447 | None:intergenic | 60.0% | |
| CCTTCTGCTAGGCCTTCAGG+TGG | - | chr5.3:27390428-27390447 | MS.gene91914:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 27389084 | 27390586 | 27389084 | ID=MS.gene91914 |
| chr5.3 | mRNA | 27389084 | 27390586 | 27389084 | ID=MS.gene91914.t1;Parent=MS.gene91914 |
| chr5.3 | exon | 27390233 | 27390586 | 27390233 | ID=MS.gene91914.t1.exon1;Parent=MS.gene91914.t1 |
| chr5.3 | CDS | 27390233 | 27390586 | 27390233 | ID=cds.MS.gene91914.t1;Parent=MS.gene91914.t1 |
| chr5.3 | exon | 27389084 | 27389422 | 27389084 | ID=MS.gene91914.t1.exon2;Parent=MS.gene91914.t1 |
| chr5.3 | CDS | 27389084 | 27389422 | 27389084 | ID=cds.MS.gene91914.t1;Parent=MS.gene91914.t1 |
| Gene Sequence |
| Protein sequence |
