Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93875.t1 | RHN82051.1 | 98.6 | 69 | 1 | 0 | 1 | 69 | 629 | 697 | 2.30E-29 | 137.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93875.t1 | Q8LPS1 | 78.5 | 65 | 14 | 0 | 1 | 65 | 632 | 696 | 1.8e-22 | 105.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93875.t1 | G7ID84 | 98.6 | 69 | 1 | 0 | 1 | 69 | 629 | 697 | 1.7e-29 | 137.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene93875 | 0.82089 | 5.40E-53 | -1.69E-46 |
| MS.gene049378 | MS.gene93875 | 0.824288 | 8.75E-54 | -1.69E-46 |
| MS.gene049424 | MS.gene93875 | 0.824271 | 8.83E-54 | -1.69E-46 |
| MS.gene049653 | MS.gene93875 | 0.805147 | 1.54E-49 | -1.69E-46 |
| MS.gene049669 | MS.gene93875 | 0.801358 | 9.35E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene93875 | 0.823388 | 1.42E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene93875 | 0.82876 | 7.52E-55 | -1.69E-46 |
| MS.gene050330 | MS.gene93875 | -0.812907 | 3.35E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene93875 | -0.800092 | 1.69E-48 | -1.69E-46 |
| MS.gene050750 | MS.gene93875 | 0.833781 | 4.38E-56 | -1.69E-46 |
| MS.gene050953 | MS.gene93875 | 0.831178 | 1.93E-55 | -1.69E-46 |
| MS.gene051349 | MS.gene93875 | 0.815891 | 7.33E-52 | -1.69E-46 |
| MS.gene051370 | MS.gene93875 | 0.836092 | 1.15E-56 | -1.69E-46 |
| MS.gene051452 | MS.gene93875 | 0.824925 | 6.20E-54 | -1.69E-46 |
| MS.gene052108 | MS.gene93875 | 0.804378 | 2.22E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene93875 | 0.863991 | 1.68E-64 | -1.69E-46 |
| MS.gene052302 | MS.gene93875 | 0.82497 | 6.05E-54 | -1.69E-46 |
| MS.gene052309 | MS.gene93875 | -0.82412 | 9.59E-54 | -1.69E-46 |
| MS.gene052358 | MS.gene93875 | -0.819274 | 1.27E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene93875 | 0.82534 | 4.94E-54 | -1.69E-46 |
| MS.gene052500 | MS.gene93875 | 0.82741 | 1.59E-54 | -1.69E-46 |
| MS.gene052502 | MS.gene93875 | 0.823734 | 1.18E-53 | -1.69E-46 |
| MS.gene052824 | MS.gene93875 | 0.823194 | 1.58E-53 | -1.69E-46 |
| MS.gene052826 | MS.gene93875 | 0.827273 | 1.71E-54 | -1.69E-46 |
| MS.gene052954 | MS.gene93875 | 0.830428 | 2.95E-55 | -1.69E-46 |
| MS.gene053006 | MS.gene93875 | 0.817887 | 2.61E-52 | -1.69E-46 |
| MS.gene053188 | MS.gene93875 | 0.811733 | 6.04E-51 | -1.69E-46 |
| MS.gene053963 | MS.gene93875 | 0.812499 | 4.11E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene93875 | 0.801684 | 8.02E-49 | -1.69E-46 |
| MS.gene054405 | MS.gene93875 | 0.823757 | 1.17E-53 | -1.69E-46 |
| MS.gene054657 | MS.gene93875 | 0.811263 | 7.64E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene93875 | 0.815844 | 7.51E-52 | -1.69E-46 |
| MS.gene05510 | MS.gene93875 | 0.800693 | 1.28E-48 | -1.69E-46 |
| MS.gene055508 | MS.gene93875 | 0.822977 | 1.77E-53 | -1.69E-46 |
| MS.gene055701 | MS.gene93875 | 0.816957 | 4.23E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene93875 | 0.8043 | 2.31E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene93875 | 0.882162 | 1.31E-70 | -1.69E-46 |
| MS.gene056979 | MS.gene93875 | 0.80056 | 1.36E-48 | -1.69E-46 |
| MS.gene057026 | MS.gene93875 | 0.817444 | 3.29E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene93875 | 0.810056 | 1.39E-50 | -1.69E-46 |
| MS.gene057079 | MS.gene93875 | 0.84669 | 1.85E-59 | -1.69E-46 |
| MS.gene05795 | MS.gene93875 | 0.84025 | 9.74E-58 | -1.69E-46 |
| MS.gene058008 | MS.gene93875 | 0.809923 | 1.49E-50 | -1.69E-46 |
| MS.gene058009 | MS.gene93875 | 0.813954 | 1.97E-51 | -1.69E-46 |
| MS.gene058037 | MS.gene93875 | 0.810244 | 1.27E-50 | -1.69E-46 |
| MS.gene058399 | MS.gene93875 | 0.803202 | 3.90E-49 | -1.69E-46 |
| MS.gene060297 | MS.gene93875 | 0.831735 | 1.41E-55 | -1.69E-46 |
| MS.gene060322 | MS.gene93875 | 0.801965 | 7.02E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene93875 | 0.83155 | 1.57E-55 | -1.69E-46 |
| MS.gene060840 | MS.gene93875 | 0.822196 | 2.70E-53 | -1.69E-46 |
| MS.gene060879 | MS.gene93875 | 0.820076 | 8.30E-53 | -1.69E-46 |
| MS.gene061040 | MS.gene93875 | -0.840158 | 1.03E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93875.t1 | MTR_1g104810 | 98.551 | 69 | 1 | 0 | 1 | 69 | 562 | 630 | 3.49e-42 | 144 |
| MS.gene93875.t1 | MTR_1g104810 | 98.551 | 69 | 1 | 0 | 1 | 69 | 629 | 697 | 1.11e-41 | 143 |
| MS.gene93875.t1 | MTR_5g009360 | 47.541 | 61 | 32 | 0 | 1 | 61 | 599 | 659 | 4.29e-13 | 62.4 |
| MS.gene93875.t1 | MTR_5g009360 | 47.541 | 61 | 32 | 0 | 1 | 61 | 473 | 533 | 4.75e-13 | 62.0 |
| MS.gene93875.t1 | MTR_5g009350 | 47.541 | 61 | 32 | 0 | 1 | 61 | 599 | 659 | 5.07e-13 | 62.0 |
| MS.gene93875.t1 | MTR_8g099420 | 50.943 | 53 | 26 | 0 | 4 | 56 | 603 | 655 | 5.83e-12 | 58.9 |
| MS.gene93875.t1 | MTR_1g034360 | 44.828 | 58 | 32 | 0 | 1 | 58 | 598 | 655 | 1.38e-11 | 58.2 |
| MS.gene93875.t1 | MTR_4g068670 | 44.828 | 58 | 32 | 0 | 1 | 58 | 596 | 653 | 2.04e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93875.t1 | AT3G05970 | 78.462 | 65 | 14 | 0 | 1 | 65 | 632 | 696 | 1.79e-30 | 111 |
| MS.gene93875.t1 | AT5G27600 | 73.438 | 64 | 17 | 0 | 1 | 64 | 632 | 695 | 3.03e-27 | 102 |
| MS.gene93875.t1 | AT1G64400 | 47.273 | 55 | 29 | 0 | 4 | 58 | 603 | 657 | 7.57e-12 | 58.9 |
| MS.gene93875.t1 | AT1G49430 | 38.710 | 62 | 38 | 0 | 1 | 62 | 602 | 663 | 5.14e-11 | 56.6 |
Find 13 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATGCCCTGTAGCTTAG+AGG | 0.270362 | 1.2:-77772037 | MS.gene93875:intron |
| GAACCATTTGCGATGGAAAA+TGG | 0.284061 | 1.2:-77771980 | MS.gene93875:CDS |
| TCAATGCTTGCCTTCATTGT+CGG | 0.421507 | 1.2:+77771947 | None:intergenic |
| TTCACAAGGGTTTCTGAGAA+GGG | 0.483679 | 1.2:+77771776 | None:intergenic |
| CCGAAGTATTCTTTCGCTTG+AGG | 0.493077 | 1.2:+77771841 | None:intergenic |
| CAAGGGTTTCTGAGAAGGGT+CGG | 0.494454 | 1.2:+77771780 | None:intergenic |
| TTTCACAAGGGTTTCTGAGA+AGG | 0.497463 | 1.2:+77771775 | None:intergenic |
| TTGGCTGAGATGGACGCTGT+TGG | 0.567107 | 1.2:-77772151 | None:intergenic |
| TCTGCTGACTCCGACAATGA+AGG | 0.578027 | 1.2:-77771957 | MS.gene93875:intron |
| GGTGGCTGAACCATTTGCGA+TGG | 0.629440 | 1.2:-77771987 | MS.gene93875:CDS |
| GAAGACCTCTCAATTTCACA+AGG | 0.638398 | 1.2:+77771762 | None:intergenic |
| CCTCAAGCGAAAGAATACTT+CGG | 0.659320 | 1.2:-77771841 | MS.gene93875:CDS |
| AAGACCTCTCAATTTCACAA+GGG | 0.689748 | 1.2:+77771763 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAATATTATTGACAATG+AGG | + | chr1.2:77771846-77771865 | None:intergenic | 20.0% |
| !!! | AGTTATTCATTTTATGTGTA+CGG | - | chr1.2:77771807-77771826 | MS.gene93875:CDS | 20.0% |
| !!! | CATTTGATTTTCAGATTAAG+AGG | - | chr1.2:77772055-77772074 | MS.gene93875:intron | 25.0% |
| !!! | AGGTTTTGAATTTGCAAAAG+CGG | - | chr1.2:77771902-77771921 | MS.gene93875:intron | 30.0% |
| ATTCAAAACCTCTAAGCTAC+AGG | + | chr1.2:77771893-77771912 | None:intergenic | 35.0% | |
| ATTGACAATGAGGCTTTACA+AGG | + | chr1.2:77771836-77771855 | None:intergenic | 35.0% | |
| TTCAAAACCTCTAAGCTACA+GGG | + | chr1.2:77771892-77771911 | None:intergenic | 35.0% | |
| ! | ATTTGCAAAAGCGGTCTTTT+TGG | - | chr1.2:77771911-77771930 | MS.gene93875:intron | 35.0% |
| CCTCAAGCGAAAGAATACTT+CGG | - | chr1.2:77772078-77772097 | MS.gene93875:intron | 40.0% | |
| GAACCATTTGCGATGGAAAA+TGG | - | chr1.2:77771939-77771958 | MS.gene93875:intron | 40.0% | |
| TCAATGCTTGCCTTCATTGT+CGG | + | chr1.2:77771975-77771994 | None:intergenic | 40.0% | |
| ! | AGACCATTTTCCATCGCAAA+TGG | + | chr1.2:77771945-77771964 | None:intergenic | 40.0% |
| TTTGATGCCCTGTAGCTTAG+AGG | - | chr1.2:77771882-77771901 | MS.gene93875:intron | 45.0% | |
| ! | CCGAAGTATTCTTTCGCTTG+AGG | + | chr1.2:77772081-77772100 | None:intergenic | 45.0% |
| ! | TGCAAAAGCGGTCTTTTTGG+TGG | - | chr1.2:77771914-77771933 | MS.gene93875:intron | 45.0% |
| ! | CAAGGGTTTCTGAGAAGGGT+CGG | + | chr1.2:77772142-77772161 | None:intergenic | 50.0% |
| !! | TCTGCTGACTCCGACAATGA+AGG | - | chr1.2:77771962-77771981 | MS.gene93875:CDS | 50.0% |
| GGTGGCTGAACCATTTGCGA+TGG | - | chr1.2:77771932-77771951 | MS.gene93875:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 77771777 | 77772164 | 77771777 | ID=MS.gene93875 |
| chr1.2 | mRNA | 77771777 | 77772164 | 77771777 | ID=MS.gene93875.t1;Parent=MS.gene93875 |
| chr1.2 | exon | 77772138 | 77772164 | 77772138 | ID=MS.gene93875.t1.exon1;Parent=MS.gene93875.t1 |
| chr1.2 | CDS | 77772138 | 77772164 | 77772138 | ID=cds.MS.gene93875.t1;Parent=MS.gene93875.t1 |
| chr1.2 | exon | 77771958 | 77772044 | 77771958 | ID=MS.gene93875.t1.exon2;Parent=MS.gene93875.t1 |
| chr1.2 | CDS | 77771958 | 77772044 | 77771958 | ID=cds.MS.gene93875.t1;Parent=MS.gene93875.t1 |
| chr1.2 | exon | 77771777 | 77771872 | 77771777 | ID=MS.gene93875.t1.exon3;Parent=MS.gene93875.t1 |
| chr1.2 | CDS | 77771777 | 77771872 | 77771777 | ID=cds.MS.gene93875.t1;Parent=MS.gene93875.t1 |
| Gene Sequence |
| Protein sequence |
