AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95476


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95476.t1 MTR_5g045160 96.216 185 7 0 1 185 480 664 1.39e-127 373
MS.gene95476.t1 MTR_5g045130 88.108 185 22 0 1 185 315 499 2.98e-117 342
MS.gene95476.t1 MTR_3g103410 86.224 196 16 1 1 185 512 707 1.25e-114 341
MS.gene95476.t1 MTR_6g445440 87.027 185 24 0 1 185 452 636 1.39e-113 337
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95476.t1 AT5G13210 63.415 205 55 1 1 185 469 673 4.11e-88 272
MS.gene95476.t1 AT5G43400 62.201 209 54 2 1 185 448 655 2.44e-84 261
MS.gene95476.t1 AT5G43390 62.679 209 53 2 1 185 436 643 2.91e-84 261
MS.gene95476.t1 AT3G24780 59.016 183 42 1 1 150 521 703 4.64e-67 218

Find 66 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCTGCTGACATTGTTCT+TGG 0.284328 5.2:-35432665 MS.gene95476:CDS
ATATGCGATCAAACACCTTC+TGG 0.312936 5.2:+35432941 None:intergenic
ATCAAGCGTCTGCAAACTCT+TGG 0.365158 5.2:-35432840 MS.gene95476:CDS
GCAGCTTCCATGGCTTCTAC+AGG 0.387857 5.2:+35432622 None:intergenic
CCTAGTGATTGCTTGGTAAT+CGG 0.388642 5.2:+35432813 None:intergenic
GCCATGGAAGCTGCCATCGC+TGG 0.399741 5.2:-35432613 MS.gene95476:CDS
GTGTGTAGCATTGGGGTTGT+TGG 0.401844 5.2:-35433106 MS.gene95476:CDS
GTTTGTGAGGAACATGGATT+GGG 0.401906 5.2:-35432980 MS.gene95476:CDS
GAAGGTGTTTGATCGCATAT+TGG 0.405938 5.2:-35432938 MS.gene95476:CDS
CTTTCTGAGTGGATGGCACC+GGG 0.421088 5.2:+35432716 None:intergenic
CACAACTAGTTTCTGATACT+CGG 0.428515 5.2:+35432588 None:intergenic
TATACTTCCTAGTGATTGCT+TGG 0.431774 5.2:+35432806 None:intergenic
AGTTTGTGAGGAACATGGAT+TGG 0.432352 5.2:-35432981 MS.gene95476:CDS
ATGGAGGTGTGTGTAGCATT+GGG 0.432777 5.2:-35433114 MS.gene95476:CDS
ACTAGGAAGTATAGAAAGAA+AGG 0.441720 5.2:-35432796 MS.gene95476:CDS
GCCAGCGATGGCAGCTTCCA+TGG 0.443302 5.2:+35432612 None:intergenic
GTTCCTCACAAACTTTGTCT+TGG 0.445968 5.2:+35432990 None:intergenic
ACAACTAGTTTCTGATACTC+GGG 0.457651 5.2:+35432589 None:intergenic
CCTTTCTGAGTGGATGGCAC+CGG 0.470831 5.2:+35432715 None:intergenic
GCCACTCCTTTCTGAGTGGA+TGG 0.483109 5.2:+35432709 None:intergenic
GCCTCAGCTTCATGTGATTG+AGG 0.484160 5.2:-35433028 MS.gene95476:CDS
TATGGAGGTGTGTGTAGCAT+TGG 0.484283 5.2:-35433115 MS.gene95476:CDS
AGGAGGATCAGATGATTAAG+AGG 0.485373 5.2:-35432891 MS.gene95476:CDS
AAGTATAGAAAGAAAGGGTA+TGG 0.487906 5.2:-35432790 MS.gene95476:CDS
GTAATAACCTTCCCTTTCCA+TGG 0.491644 5.2:+35433063 None:intergenic
GTATCAGAAACTAGTTGTGC+TGG 0.495109 5.2:-35432584 MS.gene95476:CDS
AATGCTACACACACCTCCAT+AGG 0.501130 5.2:+35433117 None:intergenic
GGAAGGTTATTACTTTCAGC+AGG 0.508350 5.2:-35433053 MS.gene95476:CDS
CCTCAGCTTCATGTGATTGA+GGG 0.511737 5.2:-35433027 MS.gene95476:CDS
CTGATACTCGGGGCCAGCGA+TGG 0.513732 5.2:+35432600 None:intergenic
CACTCAGAAAGGAGTGGCAC+TGG 0.513905 5.2:-35432704 MS.gene95476:CDS
TCAAGCGTCTGCAAACTCTT+GGG 0.514244 5.2:-35432839 MS.gene95476:CDS
CAGCTTCCATGGCTTCTACA+GGG 0.521562 5.2:+35432623 None:intergenic
ATATTGGATGTGGCTGTCAA+TGG 0.529469 5.2:-35432922 MS.gene95476:CDS
AAAGGAGTGGCACTGGTAAG+TGG 0.549583 5.2:-35432697 MS.gene95476:CDS
GACAAAGTTTGTGAGGAACA+TGG 0.551414 5.2:-35432986 MS.gene95476:CDS
TGAACGAGGAACCATGGAAA+GGG 0.554458 5.2:-35433074 MS.gene95476:CDS
TATTGGATGTGGCTGTCAAT+GGG 0.560043 5.2:-35432921 MS.gene95476:CDS
CCGATTACCAAGCAATCACT+AGG 0.565104 5.2:-35432813 MS.gene95476:CDS
CCCTCAATCACATGAAGCTG+AGG 0.568852 5.2:+35433027 None:intergenic
CCGGTGCCATCCACTCAGAA+AGG 0.571959 5.2:-35432715 MS.gene95476:CDS
GTTTGATCGCATATTGGATG+TGG 0.574432 5.2:-35432932 MS.gene95476:CDS
CTAGGAAGTATAGAAAGAAA+GGG 0.575902 5.2:-35432795 MS.gene95476:CDS
CAGTGCCACTCCTTTCTGAG+TGG 0.580391 5.2:+35432705 None:intergenic
GAGAGACTCGAAGGCCACCC+CGG 0.584902 5.2:-35432734 MS.gene95476:CDS
TTGGAATCTGAGAGACTCGA+AGG 0.585593 5.2:-35432743 MS.gene95476:CDS
GATCTTTGTCTTTAGTGACA+TGG 0.587705 5.2:-35432869 MS.gene95476:CDS
TTGAACGAGGAACCATGGAA+AGG 0.588133 5.2:-35433075 MS.gene95476:CDS
TATAAGCCCTGTAGAAGCCA+TGG 0.596604 5.2:-35432629 MS.gene95476:CDS
CTCAGCTTCATGTGATTGAG+GGG 0.599232 5.2:-35433026 MS.gene95476:CDS
ACATTGTTCTTGGATAATGA+AGG 0.599605 5.2:-35432655 MS.gene95476:CDS
TTTGTGAGGAACATGGATTG+GGG 0.600633 5.2:-35432979 MS.gene95476:CDS
CTGAATTGAACGAGGAACCA+TGG 0.603342 5.2:-35433080 MS.gene95476:CDS
CTGAGTGGATGGCACCGGGG+TGG 0.616153 5.2:+35432720 None:intergenic
GGCTGTCAATGGGAATCTCA+AGG 0.621591 5.2:-35432911 MS.gene95476:CDS
TTGTGAGGAACATGGATTGG+GGG 0.639683 5.2:-35432978 MS.gene95476:CDS
AGTCCAAGACAAAGTTTGTG+AGG 0.642724 5.2:-35432993 MS.gene95476:CDS
TGGAGGTGTGTGTAGCATTG+GGG 0.646940 5.2:-35433113 MS.gene95476:CDS
CGAGGAACCATGGAAAGGGA+AGG 0.654288 5.2:-35433070 MS.gene95476:CDS
GATGAACACTGATTTCCAGA+AGG 0.669878 5.2:-35432956 MS.gene95476:CDS
TCAGCTTCATGTGATTGAGG+GGG 0.673206 5.2:-35433025 MS.gene95476:CDS
GCCATCCACTCAGAAAGGAG+TGG 0.675952 5.2:-35432710 MS.gene95476:CDS
TTTCTGAGTGGATGGCACCG+GGG 0.698885 5.2:+35432717 None:intergenic
CAACTAGTTTCTGATACTCG+GGG 0.701259 5.2:+35432590 None:intergenic
GTTGGTGTCTGAATTGAACG+AGG 0.758083 5.2:-35433088 MS.gene95476:CDS
TGTCAATGGGAATCTCAAGG+AGG 0.758838 5.2:-35432908 MS.gene95476:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGTATAGAAAGAAAGGGTA+TGG - chr5.2:35432903-35432922 MS.gene95476:CDS 30.0%
ACATTGTTCTTGGATAATGA+AGG - chr5.2:35433038-35433057 MS.gene95476:CDS 30.0%
AGATTCCAAAAAACTATCTG+AGG + chr5.2:35432939-35432958 None:intergenic 30.0%
! ACTAGGAAGTATAGAAAGAA+AGG - chr5.2:35432897-35432916 MS.gene95476:CDS 30.0%
! CTAGGAAGTATAGAAAGAAA+GGG - chr5.2:35432898-35432917 MS.gene95476:CDS 30.0%
GATCTTTGTCTTTAGTGACA+TGG - chr5.2:35432824-35432843 MS.gene95476:CDS 35.0%
TATACTTCCTAGTGATTGCT+TGG + chr5.2:35432890-35432909 None:intergenic 35.0%
! ACAACTAGTTTCTGATACTC+GGG + chr5.2:35433107-35433126 None:intergenic 35.0%
! CACAACTAGTTTCTGATACT+CGG + chr5.2:35433108-35433127 None:intergenic 35.0%
AGGAGGATCAGATGATTAAG+AGG - chr5.2:35432802-35432821 MS.gene95476:CDS 40.0%
AGTCCAAGACAAAGTTTGTG+AGG - chr5.2:35432700-35432719 MS.gene95476:CDS 40.0%
AGTTTGTGAGGAACATGGAT+TGG - chr5.2:35432712-35432731 MS.gene95476:CDS 40.0%
ATATGCGATCAAACACCTTC+TGG + chr5.2:35432755-35432774 None:intergenic 40.0%
ATATTGGATGTGGCTGTCAA+TGG - chr5.2:35432771-35432790 MS.gene95476:CDS 40.0%
GAATCTGCTGACATTGTTCT+TGG - chr5.2:35433028-35433047 MS.gene95476:CDS 40.0%
GACAAAGTTTGTGAGGAACA+TGG - chr5.2:35432707-35432726 MS.gene95476:CDS 40.0%
GGAAGGTTATTACTTTCAGC+AGG - chr5.2:35432640-35432659 MS.gene95476:CDS 40.0%
GTAATAACCTTCCCTTTCCA+TGG + chr5.2:35432633-35432652 None:intergenic 40.0%
GTATCAGAAACTAGTTGTGC+TGG - chr5.2:35433109-35433128 MS.gene95476:CDS 40.0%
GTTCCTCACAAACTTTGTCT+TGG + chr5.2:35432706-35432725 None:intergenic 40.0%
GTTTGATCGCATATTGGATG+TGG - chr5.2:35432761-35432780 MS.gene95476:CDS 40.0%
GTTTGTGAGGAACATGGATT+GGG - chr5.2:35432713-35432732 MS.gene95476:CDS 40.0%
TATTGGATGTGGCTGTCAAT+GGG - chr5.2:35432772-35432791 MS.gene95476:CDS 40.0%
! CAACTAGTTTCTGATACTCG+GGG + chr5.2:35433106-35433125 None:intergenic 40.0%
! CCTAGTGATTGCTTGGTAAT+CGG + chr5.2:35432883-35432902 None:intergenic 40.0%
! TTTGTGAGGAACATGGATTG+GGG - chr5.2:35432714-35432733 MS.gene95476:CDS 40.0%
!! GAAGGTGTTTGATCGCATAT+TGG - chr5.2:35432755-35432774 MS.gene95476:CDS 40.0%
!! GATGAACACTGATTTCCAGA+AGG - chr5.2:35432737-35432756 MS.gene95476:CDS 40.0%
!!! CTGTGCCTCAGATAGTTTTT+TGG - chr5.2:35432931-35432950 MS.gene95476:CDS 40.0%
ATCAAGCGTCTGCAAACTCT+TGG - chr5.2:35432853-35432872 MS.gene95476:CDS 45.0%
CCGATTACCAAGCAATCACT+AGG - chr5.2:35432880-35432899 MS.gene95476:CDS 45.0%
CCTCAGCTTCATGTGATTGA+GGG - chr5.2:35432666-35432685 MS.gene95476:CDS 45.0%
CTCAGCTTCATGTGATTGAG+GGG - chr5.2:35432667-35432686 MS.gene95476:CDS 45.0%
CTGAATTGAACGAGGAACCA+TGG - chr5.2:35432613-35432632 MS.gene95476:CDS 45.0%
TATAAGCCCTGTAGAAGCCA+TGG - chr5.2:35433064-35433083 MS.gene95476:CDS 45.0%
TCAAGCGTCTGCAAACTCTT+GGG - chr5.2:35432854-35432873 MS.gene95476:CDS 45.0%
TCAGCTTCATGTGATTGAGG+GGG - chr5.2:35432668-35432687 MS.gene95476:CDS 45.0%
TGAACGAGGAACCATGGAAA+GGG - chr5.2:35432619-35432638 MS.gene95476:CDS 45.0%
TGTCAATGGGAATCTCAAGG+AGG - chr5.2:35432785-35432804 MS.gene95476:CDS 45.0%
TTGAACGAGGAACCATGGAA+AGG - chr5.2:35432618-35432637 MS.gene95476:CDS 45.0%
TTGGAATCTGAGAGACTCGA+AGG - chr5.2:35432950-35432969 MS.gene95476:CDS 45.0%
! ATGGAGGTGTGTGTAGCATT+GGG - chr5.2:35432579-35432598 MS.gene95476:CDS 45.0%
! GTTGGTGTCTGAATTGAACG+AGG - chr5.2:35432605-35432624 MS.gene95476:CDS 45.0%
!! TTGTGAGGAACATGGATTGG+GGG - chr5.2:35432715-35432734 MS.gene95476:CDS 45.0%
CAGCTTCCATGGCTTCTACA+GGG + chr5.2:35433073-35433092 None:intergenic 50.0%
CCCTCAATCACATGAAGCTG+AGG + chr5.2:35432669-35432688 None:intergenic 50.0%
GCCTCAGCTTCATGTGATTG+AGG - chr5.2:35432665-35432684 MS.gene95476:CDS 50.0%
GGCTGTCAATGGGAATCTCA+AGG - chr5.2:35432782-35432801 MS.gene95476:CDS 50.0%
! GTGTGTAGCATTGGGGTTGT+TGG - chr5.2:35432587-35432606 MS.gene95476:CDS 50.0%
! TGGAGGTGTGTGTAGCATTG+GGG - chr5.2:35432580-35432599 MS.gene95476:CDS 50.0%
!! AAAGGAGTGGCACTGGTAAG+TGG - chr5.2:35432996-35433015 MS.gene95476:CDS 50.0%
CACTCAGAAAGGAGTGGCAC+TGG - chr5.2:35432989-35433008 MS.gene95476:CDS 55.0%
CCTTTCTGAGTGGATGGCAC+CGG + chr5.2:35432981-35433000 None:intergenic 55.0%
CGAGGAACCATGGAAAGGGA+AGG - chr5.2:35432623-35432642 MS.gene95476:CDS 55.0%
CTTTCTGAGTGGATGGCACC+GGG + chr5.2:35432980-35432999 None:intergenic 55.0%
GCAGCTTCCATGGCTTCTAC+AGG + chr5.2:35433074-35433093 None:intergenic 55.0%
GCCACTCCTTTCTGAGTGGA+TGG + chr5.2:35432987-35433006 None:intergenic 55.0%
GCCATCCACTCAGAAAGGAG+TGG - chr5.2:35432983-35433002 MS.gene95476:CDS 55.0%
TTTCTGAGTGGATGGCACCG+GGG + chr5.2:35432979-35432998 None:intergenic 55.0%
! CAGTGCCACTCCTTTCTGAG+TGG + chr5.2:35432991-35433010 None:intergenic 55.0%
!! CCGGTGCCATCCACTCAGAA+AGG - chr5.2:35432978-35432997 MS.gene95476:CDS 60.0%
CTGATACTCGGGGCCAGCGA+TGG + chr5.2:35433096-35433115 None:intergenic 65.0%
GAGAGACTCGAAGGCCACCC+CGG - chr5.2:35432959-35432978 MS.gene95476:CDS 65.0%
GCCAGCGATGGCAGCTTCCA+TGG + chr5.2:35433084-35433103 None:intergenic 65.0%
GCCATGGAAGCTGCCATCGC+TGG - chr5.2:35433080-35433099 MS.gene95476:CDS 65.0%
! CTGAGTGGATGGCACCGGGG+TGG + chr5.2:35432976-35432995 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 35432579 35433136 35432579 ID=MS.gene95476
chr5.2 mRNA 35432579 35433136 35432579 ID=MS.gene95476.t1;Parent=MS.gene95476
chr5.2 exon 35432579 35433136 35432579 ID=MS.gene95476.t1.exon1;Parent=MS.gene95476.t1
chr5.2 CDS 35432579 35433136 35432579 ID=cds.MS.gene95476.t1;Parent=MS.gene95476.t1
Gene Sequence

>MS.gene95476

ATGGAGGTGTGTGTAGCATTGGGGTTGTTGGTGTCTGAATTGAACGAGGAACCATGGAAAGGGAAGGTTATTACTTTCAGCAGGAAGCCTCAGCTTCATGTGATTGAGGGGGATGATCTGAAGTCCAAGACAAAGTTTGTGAGGAACATGGATTGGGGGATGAACACTGATTTCCAGAAGGTGTTTGATCGCATATTGGATGTGGCTGTCAATGGGAATCTCAAGGAGGATCAGATGATTAAGAGGATCTTTGTCTTTAGTGACATGGAGTTTGATCAAGCGTCTGCAAACTCTTGGGAGACCGATTACCAAGCAATCACTAGGAAGTATAGAAAGAAAGGGTATGGTTCTGCTGTGCCTCAGATAGTTTTTTGGAATCTGAGAGACTCGAAGGCCACCCCGGTGCCATCCACTCAGAAAGGAGTGGCACTGGTAAGTGGTTTTTCGAAGAATCTGCTGACATTGTTCTTGGATAATGAAGGTGATATAAGCCCTGTAGAAGCCATGGAAGCTGCCATCGCTGGCCCCGAGTATCAGAAACTAGTTGTGCTGGATTGA

Protein sequence

>MS.gene95476.t1

MEVCVALGLLVSELNEEPWKGKVITFSRKPQLHVIEGDDLKSKTKFVRNMDWGMNTDFQKVFDRILDVAVNGNLKEDQMIKRIFVFSDMEFDQASANSWETDYQAITRKYRKKGYGSAVPQIVFWNLRDSKATPVPSTQKGVALVSGFSKNLLTLFLDNEGDISPVEAMEAAIAGPEYQKLVVLD