AlfalfaGEDB | MsG0180000481.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000481.01

KEGG: K14686
GO: GO:0005375 GO:0016021 GO:0035434
PFAM: PF04145

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000481.01.T02	XP_003588901.1	95.556	45	2	0	1	45	98	142	2.03E-21	90.1
MsG0180000481.01.T01	XP_003588901.1	94.406	143	7	1	1	143	1	142	2.73E-94	278

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000481.01.T01	Q93VM8	54.422	147	62	3	1	143	1	146	5.61E-49	157

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000481.01.T01	G7I8Y2	94.406	143	7	1	1	143	1	142	1.30e-94	278

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049040.01	MsG0180000481.01	0.816475	5.426198e-52	3.848692e-49
MsG0080049068.01	MsG0180000481.01	0.821464	3.981812e-53	3.241688e-50
MsG0180000114.01	MsG0180000481.01	0.807002	6.252281e-50	3.443153e-47
MsG0180000246.01	MsG0180000481.01	0.803697	3.080641e-49	1.558423e-46
MsG0180000481.01	MsG0180000482.01	0.831919	1.270239e-55	1.398388e-52
MsG0180000481.01	MsG0180001085.01	0.808102	3.651607e-50	2.069518e-47
MsG0180000481.01	MsG0180001100.01	0.822426	2.383514e-53	1.994474e-50
MsG0180000481.01	MsG0180001270.01	0.815406	9.395865e-52	6.472264e-49
MsG0180000481.01	MsG0180003353.01	0.802022	6.831937e-49	3.311328e-46
MsG0180000481.01	MsG0180004448.01	0.826883	2.123798e-54	2.017343e-51
MsG0180000481.01	MsG0180004493.01	0.810022	1.417004e-50	8.449372e-48
MsG0180000481.01	MsG0180004735.01	0.823024	1.730617e-53	1.472899e-50
MsG0180000481.01	MsG0180005397.01	0.814582	1.431765e-51	9.644540e-49
MsG0180000481.01	MsG0180005418.01	0.803418	3.519782e-49	1.767863e-46
MsG0180000481.01	MsG0180005807.01	0.800820	1.204673e-48	5.663780e-46
MsG0180000481.01	MsG0280007062.01	0.815438	9.242271e-52	6.372285e-49
MsG0180000481.01	MsG0280008932.01	0.813464	2.526224e-51	1.651005e-48
MsG0180000481.01	MsG0280009685.01	0.804723	1.883936e-49	9.785954e-47
MsG0180000481.01	MsG0280010564.01	0.820151	7.980032e-53	6.264681e-50
MsG0180000481.01	MsG0280010975.01	0.815317	9.833665e-52	6.757364e-49
MsG0180000481.01	MsG0380016194.01	0.835670	1.465947e-56	1.805121e-53
MsG0180000481.01	MsG0380016284.01	0.804770	1.841463e-49	9.576695e-47
MsG0180000481.01	MsG0480018848.01	0.810808	9.589165e-51	5.837549e-48
MsG0180000481.01	MsG0480020690.01	0.827786	1.290350e-54	1.258315e-51
MsG0180000481.01	MsG0480020783.01	0.818481	1.916675e-52	1.436453e-49
MsG0180000481.01	MsG0480022537.01	0.802821	4.675942e-49	2.313146e-46
MsG0180000481.01	MsG0480022624.01	0.813115	3.014232e-51	1.951014e-48
MsG0180000481.01	MsG0480022643.01	0.828683	7.845231e-55	7.853508e-52
MsG0180000481.01	MsG0480023381.01	0.818187	2.234152e-52	1.660815e-49
MsG0180000481.01	MsG0480023384.01	0.804794	1.820139e-49	9.471797e-47
MsG0180000481.01	MsG0580024873.01	0.826272	2.970611e-54	2.772847e-51
MsG0180000481.01	MsG0580028024.01	0.843829	1.101132e-58	1.744145e-55
MsG0180000481.01	MsG0580028723.01	0.807459	5.002684e-50	2.788016e-47
MsG0180000481.01	MsG0580029414.01	0.837122	6.261446e-57	8.054636e-54
MsG0180000481.01	MsG0680030464.01	0.811224	7.790365e-51	4.795159e-48
MsG0180000481.01	MsG0680031469.01	0.831478	1.631626e-55	1.772788e-52
MsG0180000481.01	MsG0680032830.01	0.811910	5.526241e-51	3.463692e-48
MsG0180000481.01	MsG0680033377.01	0.810412	1.167369e-50	7.031761e-48
MsG0180000481.01	MsG0680034064.01	0.834837	2.379069e-56	2.857243e-53
MsG0180000481.01	MsG0680034767.01	0.815148	1.072477e-51	7.335187e-49
MsG0180000481.01	MsG0680035307.01	0.852832	3.562768e-61	7.542376e-58
MsG0180000481.01	MsG0680035731.01	0.804916	1.717124e-49	8.964169e-47
MsG0180000481.01	MsG0780037330.01	0.814427	1.549507e-51	1.039418e-48
MsG0180000481.01	MsG0780037399.01	0.808399	3.157286e-50	1.803460e-47
MsG0180000481.01	MsG0780037662.01	0.813281	2.771772e-51	1.802323e-48
MsG0180000481.01	MsG0780037701.01	0.808399	3.156333e-50	1.802957e-47
MsG0180000481.01	MsG0780039975.01	0.832391	9.711688e-56	1.083991e-52
MsG0180000481.01	MsG0880045044.01	0.811804	5.828148e-51	3.642646e-48
MsG0180000481.01	MsG0880046723.01	0.810093	1.368401e-50	8.173960e-48
MsG0180000481.01	MsG0880046736.01	0.814532	1.468847e-51	9.881022e-49
MsG0180000481.01	MsG0880046765.01	0.803377	3.588949e-49	1.800643e-46
MsG0180000481.01	MsG0880047152.01	0.808895	2.473163e-50	1.431090e-47
MsG0180000481.01	MsG0880047703.01	0.808528	2.962932e-50	1.698148e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000481.01.T02	MTR_1g015000	95.556	45	2	0	1	45	98	142	2.46e-25	90.1
MsG0180000481.01.T02	MTR_3g105330	71.111	45	11	1	1	45	101	143	2.92e-16	67.0
MsG0180000481.01.T01	MTR_1g015000	94.406	143	7	1	1	143	1	142	3.30e-98	278
MsG0180000481.01.T01	MTR_3g105330	59.060	149	49	4	1	143	1	143	2.74e-55	170
MsG0180000481.01.T01	MTR_4g019870	31.724	145	70	4	2	138	13	136	4.27e-18	75.5
MsG0180000481.01.T01	MTR_7g066070	32.353	136	65	3	2	131	22	136	7.88e-16	69.7
MsG0180000481.01.T01	MTR_0027s0220	30.000	140	76	4	2	139	27	146	1.37e-14	66.6
MsG0180000481.01.T01	MTR_4g065660	29.839	124	65	3	5	128	35	136	1.40e-13	64.3
MsG0180000481.01.T01	MTR_4g065123	28.455	123	66	2	1	123	27	127	2.34e-13	63.2
MsG0180000481.01.T01	MTR_4g064963	31.579	133	66	4	5	133	46	157	2.72e-13	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000481.01.T01	AT5G20650	54.422	147	62	3	1	143	1	146	5.72e-50	157
MsG0180000481.01.T01	AT2G26975	34.646	127	61	2	1	126	24	129	1.90e-19	79.3
MsG0180000481.01.T01	AT5G59040	34.646	127	62	2	1	127	29	134	7.27e-17	72.8
MsG0180000481.01.T01	AT2G37925	30.469	128	71	2	1	128	29	138	1.69e-13	63.9
MsG0180000481.01.T01	AT5G59030	28.472	144	79	3	5	142	45	170	2.00e-13	64.3
MsG0180000481.01.T01	AT3G46900	26.761	142	78	4	7	142	35	156	1.68e-12	61.6

Find 31 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAACATTCTACCAATATT+TGG	0.138215	1:-6673523	MsG0180000481.01.T01:CDS
GAGATTCGGACTCCTCTTCT+TGG	0.249119	1:-6673438	MsG0180000481.01.T01:CDS
ACCAATATTTGGAAAATATT+CGG	0.254322	1:-6673512	MsG0180000481.01.T01:CDS
TCCTCTTCTTGGGTTGAATA+AGG	0.262656	1:-6673427	MsG0180000481.01.T01:CDS
TGACACTCTTATTTGATTCA+TGG	0.286816	1:-6673599	MsG0180000481.01.T01:CDS
TGATGCACATGACATTATAT+TGG	0.315744	1:-6673632	MsG0180000481.01.T01:CDS
GATGCACATGACATTATATT+GGG	0.333053	1:-6673631	MsG0180000481.01.T01:CDS
CGTTTGGTTACTTCTTGTTT+AGG	0.336421	1:-6673281	MsG0180000481.01.T01:CDS
ATTCATGGAAAACCAATTCA+TGG	0.342364	1:-6673584	MsG0180000481.01.T01:CDS
TTGGCTATAATGTCGTTTAA+TGG	0.343696	1:-6673336	MsG0180000481.01.T01:CDS
TTTGGTGTGAATTCTGCTAT+TGG	0.364233	1:-6673372	MsG0180000481.01.T01:CDS
ATTGTGGTTGGTTTAACGTT+TGG	0.388729	1:-6673297	MsG0180000481.01.T01:CDS
TAGATAACTCGTCCATGAAT+TGG	0.393378	1:+6673572	None:intergenic
AGATTCGGACTCCTCTTCTT+GGG	0.397569	1:-6673437	MsG0180000481.01.T01:CDS
TTGGTGTGAATTCTGCTATT+GGG	0.404245	1:-6673371	MsG0180000481.01.T01:CDS
ATTTGGAAAATATTCGGATT+CGG	0.414816	1:-6673506	MsG0180000481.01.T01:CDS
TATTGGGTATTTGTTGATGT+TGG	0.437573	1:-6673355	MsG0180000481.01.T01:CDS
AGTCGCCAGTGCCGGAGATT+CGG	0.441947	1:-6673452	MsG0180000481.01.T01:CDS
GAGGAACAAGGTTATTGAAT+CGG	0.464562	1:-6673400	MsG0180000481.01.T01:CDS
TACTTCTTGTTTAGGAGTCA+AGG	0.469886	1:-6673273	MsG0180000481.01.T01:CDS
TTGGGTTGAATAAGGTTTCG+AGG	0.486516	1:-6673419	MsG0180000481.01.T01:CDS
AGAAGAGGAGTCCGAATCTC+CGG	0.488903	1:+6673441	None:intergenic
TAGGAGTCAAGGTGAGGAAG+AGG	0.504862	1:-6673262	MsG0180000481.01.T01:CDS
GGAGTCCGAATCTCCGGCAC+TGG	0.516590	1:+6673447	None:intergenic
GAGGACGCAGTCGCCAGTGC+CGG	0.527555	1:-6673460	MsG0180000481.01.T01:CDS
ACCTTATTCAACCCAAGAAG+AGG	0.545808	1:+6673426	None:intergenic
TCAAGGTGAGGAAGAGGCTG+CGG	0.577917	1:-6673256	MsG0180000481.01.T01:CDS
GAATAAGGTTTCGAGGAACA+AGG	0.583225	1:-6673412	MsG0180000481.01.T01:CDS
GCTATAATGTCGTTTAATGG+TGG	0.610087	1:-6673333	MsG0180000481.01.T01:CDS
CTTGTTTAGGAGTCAAGGTG+AGG	0.615505	1:-6673268	MsG0180000481.01.T01:CDS
ATGCACATGACATTATATTG+GGG	0.668922	1:-6673630	MsG0180000481.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACCAATATTTGGAAAATATT+CGG	-	Chr1:6673345-6673364	MsG0180000481.01.T01:CDS	20.0%
!	ATTTGGAAAATATTCGGATT+CGG	-	Chr1:6673351-6673370	MsG0180000481.01.T01:CDS	25.0%
!!	TCCGAATATTTTCCAAATAT+TGG	+	Chr1:6673349-6673368	None:intergenic	25.0%
!!!	GTTATTGAATCGGTTTTATT+TGG	-	Chr1:6673467-6673486	MsG0180000481.01.T01:CDS	25.0%
	ATGCACATGACATTATATTG+GGG	-	Chr1:6673227-6673246	MsG0180000481.01.T01:CDS	30.0%
	ATTCATGGAAAACCAATTCA+TGG	-	Chr1:6673273-6673292	MsG0180000481.01.T01:CDS	30.0%
	GATGCACATGACATTATATT+GGG	-	Chr1:6673226-6673245	MsG0180000481.01.T01:CDS	30.0%
	TGATGCACATGACATTATAT+TGG	-	Chr1:6673225-6673244	MsG0180000481.01.T01:CDS	30.0%
	TGCAACATTCTACCAATATT+TGG	-	Chr1:6673334-6673353	MsG0180000481.01.T01:CDS	30.0%
!	TATTGGGTATTTGTTGATGT+TGG	-	Chr1:6673502-6673521	MsG0180000481.01.T01:CDS	30.0%
!	TGACACTCTTATTTGATTCA+TGG	-	Chr1:6673258-6673277	MsG0180000481.01.T01:CDS	30.0%
!	TTGGCTATAATGTCGTTTAA+TGG	-	Chr1:6673521-6673540	MsG0180000481.01.T01:CDS	30.0%
	GAGGAACAAGGTTATTGAAT+CGG	-	Chr1:6673457-6673476	MsG0180000481.01.T01:CDS	35.0%
	TAGATAACTCGTCCATGAAT+TGG	+	Chr1:6673288-6673307	None:intergenic	35.0%
	TTGGTGTGAATTCTGCTATT+GGG	-	Chr1:6673486-6673505	MsG0180000481.01.T01:CDS	35.0%
!	ATTCGGTTGAAGTTTTTCTC+TGG	-	Chr1:6673368-6673387	MsG0180000481.01.T01:CDS	35.0%
!	CGTTTGGTTACTTCTTGTTT+AGG	-	Chr1:6673576-6673595	MsG0180000481.01.T01:CDS	35.0%
!	GCTATAATGTCGTTTAATGG+TGG	-	Chr1:6673524-6673543	MsG0180000481.01.T01:CDS	35.0%
!	TACTTCTTGTTTAGGAGTCA+AGG	-	Chr1:6673584-6673603	MsG0180000481.01.T01:CDS	35.0%
!	TTGAAGTTTTTCTCTGGTGA+GGG	-	Chr1:6673374-6673393	MsG0180000481.01.T01:CDS	35.0%
!	TTTGGTGTGAATTCTGCTAT+TGG	-	Chr1:6673485-6673504	MsG0180000481.01.T01:CDS	35.0%
!!	ATTGTGGTTGGTTTAACGTT+TGG	-	Chr1:6673560-6673579	MsG0180000481.01.T01:CDS	35.0%
!!	GTTTAATGGTGGTGTTTTTG+TGG	-	Chr1:6673535-6673554	MsG0180000481.01.T01:CDS	35.0%
	ACCTTATTCAACCCAAGAAG+AGG	+	Chr1:6673434-6673453	None:intergenic	40.0%
	GAATAAGGTTTCGAGGAACA+AGG	-	Chr1:6673445-6673464	MsG0180000481.01.T01:CDS	40.0%
!	GTTGAAGTTTTTCTCTGGTG+AGG	-	Chr1:6673373-6673392	MsG0180000481.01.T01:CDS	40.0%
!	GTTTTTGTGGCTATTGTGGT+TGG	-	Chr1:6673548-6673567	MsG0180000481.01.T01:CDS	40.0%
!	TGAAGTTTTTCTCTGGTGAG+GGG	-	Chr1:6673375-6673394	MsG0180000481.01.T01:CDS	40.0%
!!	TCCTCTTCTTGGGTTGAATA+AGG	-	Chr1:6673430-6673449	MsG0180000481.01.T01:CDS	40.0%
!!	TGGTGTTTTTGTGGCTATTG+TGG	-	Chr1:6673544-6673563	MsG0180000481.01.T01:CDS	40.0%
!!	TTGGGTTGAATAAGGTTTCG+AGG	-	Chr1:6673438-6673457	MsG0180000481.01.T01:CDS	40.0%
	AGATTCGGACTCCTCTTCTT+GGG	-	Chr1:6673420-6673439	MsG0180000481.01.T01:CDS	45.0%
	CTTGTTTAGGAGTCAAGGTG+AGG	-	Chr1:6673589-6673608	MsG0180000481.01.T01:CDS	45.0%
	AGAAGAGGAGTCCGAATCTC+CGG	+	Chr1:6673419-6673438	None:intergenic	50.0%
	GAGATTCGGACTCCTCTTCT+TGG	-	Chr1:6673419-6673438	MsG0180000481.01.T01:CDS	50.0%
	TAGGAGTCAAGGTGAGGAAG+AGG	-	Chr1:6673595-6673614	MsG0180000481.01.T01:CDS	50.0%
!	AGTTTTTCTCTGGTGAGGGG+AGG	-	Chr1:6673378-6673397	MsG0180000481.01.T01:CDS	50.0%
	TCAAGGTGAGGAAGAGGCTG+CGG	-	Chr1:6673601-6673620	MsG0180000481.01.T01:CDS	55.0%
!	AGTCGCCAGTGCCGGAGATT+CGG	-	Chr1:6673405-6673424	MsG0180000481.01.T01:CDS	60.0%
	GGAGTCCGAATCTCCGGCAC+TGG	+	Chr1:6673413-6673432	None:intergenic	65.0%
	GAGGACGCAGTCGCCAGTGC+CGG	-	Chr1:6673397-6673416	MsG0180000481.01.T01:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	6673224	6673655	6673224	ID=MsG0180000481.01;Name=MsG0180000481.01
Chr1	mRNA	6673224	6673655	6673224	ID=MsG0180000481.01.T01;Parent=MsG0180000481.01;Name=MsG0180000481.01.T01;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|143
Chr1	exon	6673224	6673655	6673224	ID=MsG0180000481.01.T01:exon:2418;Parent=MsG0180000481.01.T01
Chr1	CDS	6673224	6673655	6673224	ID=MsG0180000481.01.T01:cds;Parent=MsG0180000481.01.T01
Chr1	mRNA	6673224	6673361	6673224	ID=MsG0180000481.01.T02;Parent=MsG0180000481.01;Name=MsG0180000481.01.T02;_AED=0.35;_eAED=0.35;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|45
Chr1	exon	6673224	6673361	6673224	ID=MsG0180000481.01.T02:exon:2419;Parent=MsG0180000481.01.T02
Chr1	CDS	6673224	6673361	6673224	ID=MsG0180000481.01.T02:cds;Parent=MsG0180000481.01.T02

Gene Sequence

>MsG0180000481.01.T02

ATGTTGGCTATAATGTCGTTTAATGGTGGTGTTTTTGTGGCTATTGTGGTTGGTTTAACGTTTGGTTACTTCTTGTTTAGGAGTCAAGGTGAGGAAGAGGCTGCGGTTGTTGTTGATAATTCTTGTGCATGTGCTTAG

>MsG0180000481.01.T01

ATGATGCACATGACATTATATTGGGGAAAAAACGTGACACTCTTATTTGATTCATGGAAAACCAATTCATGGACGAGTTATCTACTGAGTTTAATTTCATGTTTCATGATTGCAACATTCTACCAATATTTGGAAAATATTCGGATTCGGTTGAAGTTTTTCTCTGGTGAGGGGAGGACGCAGTCGCCAGTGCCGGAGATTCGGACTCCTCTTCTTGGGTTGAATAAGGTTTCGAGGAACAAGGTTATTGAATCGGTTTTATTTGGTGTGAATTCTGCTATTGGGTATTTGTTGATGTTGGCTATAATGTCGTTTAATGGTGGTGTTTTTGTGGCTATTGTGGTTGGTTTAACGTTTGGTTACTTCTTGTTTAGGAGTCAAGGTGAGGAAGAGGCTGCGGTTGTTGTTGATAATTCTTGTGCATGTGCTTAG

Protein sequence

>MsG0180000481.01.T02

MLAIMSFNGGVFVAIVVGLTFGYFLFRSQGEEEAAVVVDNSCACA*

>MsG0180000481.01.T01

MMHMTLYWGKNVTLLFDSWKTNSWTSYLLSLISCFMIATFYQYLENIRIRLKFFSGEGRTQSPVPEIRTPLLGLNKVSRNKVIESVLFGVNSAIGYLLMLAIMSFNGGVFVAIVVGLTFGYFLFRSQGEEEAAVVVDNSCACA*