AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046736.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046736.01.T01 MTR_4g113750 96.262 107 4 0 1 107 1 107 1.03e-73 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046736.01.T01 AT1G75125 60.638 94 37 0 13 106 11 104 1.20e-39 128

Find 29 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGAAATCGCCTTGGAAT+TGG 0.320897 8:-77543566 None:intergenic
GTCCTACTTGCGATGGAAAC+AGG 0.354255 8:+77543908 MsG0880046736.01.T01:CDS
ACTTTGTGTCCGAGAATAAA+CGG 0.396004 8:+77543669 MsG0880046736.01.T01:CDS
AATCTTTACCCGTTTATTCT+CGG 0.396090 8:-77543678 None:intergenic
CGATTTCCAACATGGATTAC+CGG 0.398072 8:+77543578 MsG0880046736.01.T01:CDS
ACAACGTTGTCTGAATTGTC+TGG 0.418889 8:+77544049 MsG0880046736.01.T01:CDS
ACACATGGGTTAATTGCAAC+AGG 0.458488 8:-77543883 None:intergenic
CTTTGTGTCCGAGAATAAAC+GGG 0.475435 8:+77543670 MsG0880046736.01.T01:CDS
TGTCTGAATTGTCTGGGAAA+AGG 0.496121 8:+77544056 MsG0880046736.01.T01:CDS
TTCCAAGGCGATTTCCAACA+TGG 0.513408 8:+77543570 MsG0880046736.01.T01:CDS
ATCCATGTTGGAAATCGCCT+TGG 0.531843 8:-77543572 None:intergenic
ATATATGCAAGTTATGCAGC+GGG 0.546291 8:+77543827 MsG0880046736.01.T01:CDS
TGTAAAAGTTGTAGAGGGAA+AGG 0.556567 8:+77543799 MsG0880046736.01.T01:CDS
GTAAAAGTTGTAGAGGGAAA+GGG 0.562827 8:+77543800 MsG0880046736.01.T01:CDS
CAACGTTGTCTGAATTGTCT+GGG 0.565752 8:+77544050 MsG0880046736.01.T01:CDS
CCCCAATATTACAAAAGCAC+CGG 0.566726 8:-77543597 None:intergenic
GCGGGAATTCAACCATAGCT+TGG 0.577529 8:+77543845 MsG0880046736.01.T01:CDS
AAAGCACCGGTAATCCATGT+TGG 0.590454 8:-77543584 None:intergenic
TGTGTTTGTCCTACTTGCGA+TGG 0.598415 8:+77543901 MsG0880046736.01.T01:CDS
TTGCCGTGTAAAAGTTGTAG+AGG 0.598560 8:+77543793 MsG0880046736.01.T01:CDS
ATTGCAACAGGATCATACAT+TGG 0.599710 8:-77543871 None:intergenic
AAGAAATGAATCCAATTCCA+AGG 0.602574 8:+77543555 None:intergenic
ATACATTGGAGACCAAGCTA+TGG 0.629079 8:-77543857 None:intergenic
TCGCAAGTAGGACAAACACA+TGG 0.629902 8:-77543898 None:intergenic
GGGAATTGCAACTCTGAACG+TGG 0.644672 8:+77543619 MsG0880046736.01.T01:CDS
TATATATGCAAGTTATGCAG+CGG 0.648101 8:+77543826 MsG0880046736.01.T01:CDS
TGCCGTGTAAAAGTTGTAGA+GGG 0.654823 8:+77543794 MsG0880046736.01.T01:CDS
CGCAAGTAGGACAAACACAT+GGG 0.656170 8:-77543897 None:intergenic
AACCTGTTTCCATCGCAAGT+AGG 0.668457 8:-77543910 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATTATAGTAACTGATAA+TGG + Chr8:77544012-77544031 MsG0880046736.01.T01:intron 20.0%
!! TTTGCTTTCATTTGAATATT+GGG + Chr8:77543960-77543979 MsG0880046736.01.T01:intron 20.0%
!!! TTTTGCTTTCATTTGAATAT+TGG + Chr8:77543959-77543978 MsG0880046736.01.T01:intron 20.0%
! GATAATATTGATATGCTTTC+TGG + Chr8:77543737-77543756 MsG0880046736.01.T01:intron 25.0%
! TTGCTTTCATTTGAATATTG+GGG + Chr8:77543961-77543980 MsG0880046736.01.T01:intron 25.0%
AATCTTTACCCGTTTATTCT+CGG - Chr8:77543681-77543700 None:intergenic 30.0%
TATATATGCAAGTTATGCAG+CGG + Chr8:77543826-77543845 MsG0880046736.01.T01:CDS 30.0%
TGTCTTGAAATTGAATTGCA+AGG + Chr8:77543990-77544009 MsG0880046736.01.T01:intron 30.0%
! TTGATATGCTTTCTGGAAAT+TGG + Chr8:77543744-77543763 MsG0880046736.01.T01:intron 30.0%
ACTTTGTGTCCGAGAATAAA+CGG + Chr8:77543669-77543688 MsG0880046736.01.T01:CDS 35.0%
ATATATGCAAGTTATGCAGC+GGG + Chr8:77543827-77543846 MsG0880046736.01.T01:CDS 35.0%
ATTGCAACAGGATCATACAT+TGG - Chr8:77543874-77543893 None:intergenic 35.0%
GTAAAAGTTGTAGAGGGAAA+GGG + Chr8:77543800-77543819 MsG0880046736.01.T01:CDS 35.0%
TGTAAAAGTTGTAGAGGGAA+AGG + Chr8:77543799-77543818 MsG0880046736.01.T01:CDS 35.0%
! ACCGGTGCTTTTGTAATATT+GGG + Chr8:77543596-77543615 MsG0880046736.01.T01:CDS 35.0%
! TACCGGTGCTTTTGTAATAT+TGG + Chr8:77543595-77543614 MsG0880046736.01.T01:CDS 35.0%
! TTCCCTCTACAACTTTTACA+CGG - Chr8:77543799-77543818 None:intergenic 35.0%
!!! ACTGATAATGGTTTTGTTGC+AGG + Chr8:77544024-77544043 MsG0880046736.01.T01:intron 35.0%
!!! CTGATAATGGTTTTGTTGCA+GGG + Chr8:77544025-77544044 MsG0880046736.01.T01:intron 35.0%
ACAACGTTGTCTGAATTGTC+TGG + Chr8:77544049-77544068 MsG0880046736.01.T01:CDS 40.0%
ATACATTGGAGACCAAGCTA+TGG - Chr8:77543860-77543879 None:intergenic 40.0%
CAACGTTGTCTGAATTGTCT+GGG + Chr8:77544050-77544069 MsG0880046736.01.T01:CDS 40.0%
CTTTGTGTCCGAGAATAAAC+GGG + Chr8:77543670-77543689 MsG0880046736.01.T01:CDS 40.0%
TGCCGTGTAAAAGTTGTAGA+GGG + Chr8:77543794-77543813 MsG0880046736.01.T01:CDS 40.0%
TGTCTGAATTGTCTGGGAAA+AGG + Chr8:77544056-77544075 MsG0880046736.01.T01:CDS 40.0%
TTGCCGTGTAAAAGTTGTAG+AGG + Chr8:77543793-77543812 MsG0880046736.01.T01:CDS 40.0%
! ACACATGGGTTAATTGCAAC+AGG - Chr8:77543886-77543905 None:intergenic 40.0%
! CCCCAATATTACAAAAGCAC+CGG - Chr8:77543600-77543619 None:intergenic 40.0%
! CCGGTGCTTTTGTAATATTG+GGG + Chr8:77543597-77543616 MsG0880046736.01.T01:CDS 40.0%
! CGATTTCCAACATGGATTAC+CGG + Chr8:77543578-77543597 MsG0880046736.01.T01:CDS 40.0%
! CGGTGCTTTTGTAATATTGG+GGG + Chr8:77543598-77543617 MsG0880046736.01.T01:CDS 40.0%
! GGTGCTTTTGTAATATTGGG+GGG + Chr8:77543599-77543618 MsG0880046736.01.T01:CDS 40.0%
AACCTGTTTCCATCGCAAGT+AGG - Chr8:77543913-77543932 None:intergenic 45.0%
ATCCATGTTGGAAATCGCCT+TGG - Chr8:77543575-77543594 None:intergenic 45.0%
CGCAAGTAGGACAAACACAT+GGG - Chr8:77543900-77543919 None:intergenic 45.0%
GTTGGAAATCGCCTTGGAAT+TGG - Chr8:77543569-77543588 None:intergenic 45.0%
TCGCAAGTAGGACAAACACA+TGG - Chr8:77543901-77543920 None:intergenic 45.0%
TGTGTTTGTCCTACTTGCGA+TGG + Chr8:77543901-77543920 MsG0880046736.01.T01:CDS 45.0%
TTCCAAGGCGATTTCCAACA+TGG + Chr8:77543570-77543589 MsG0880046736.01.T01:CDS 45.0%
!! AAAGCACCGGTAATCCATGT+TGG - Chr8:77543587-77543606 None:intergenic 45.0%
GCGGGAATTCAACCATAGCT+TGG + Chr8:77543845-77543864 MsG0880046736.01.T01:CDS 50.0%
GGGAATTGCAACTCTGAACG+TGG + Chr8:77543619-77543638 MsG0880046736.01.T01:CDS 50.0%
GTCCTACTTGCGATGGAAAC+AGG + Chr8:77543908-77543927 MsG0880046736.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 77543560 77544088 77543560 ID=MsG0880046736.01;Name=MsG0880046736.01
Chr8 mRNA 77543560 77544088 77543560 ID=MsG0880046736.01.T01;Parent=MsG0880046736.01;Name=MsG0880046736.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|107
Chr8 exon 77543560 77543691 77543560 ID=MsG0880046736.01.T01:exon:33178;Parent=MsG0880046736.01.T01
Chr8 exon 77543781 77543929 77543781 ID=MsG0880046736.01.T01:exon:33179;Parent=MsG0880046736.01.T01
Chr8 exon 77544046 77544088 77544046 ID=MsG0880046736.01.T01:exon:33180;Parent=MsG0880046736.01.T01
Chr8 CDS 77543560 77543691 77543560 ID=MsG0880046736.01.T01:cds;Parent=MsG0880046736.01.T01
Chr8 CDS 77543781 77543929 77543781 ID=MsG0880046736.01.T01:cds;Parent=MsG0880046736.01.T01
Chr8 CDS 77544046 77544088 77544046 ID=MsG0880046736.01.T01:cds;Parent=MsG0880046736.01.T01
Gene Sequence

>MsG0880046736.01.T01

ATGAATCCAATTCCAAGGCGATTTCCAACATGGATTACCGGTGCTTTTGTAATATTGGGGGGAATTGCAACTCTGAACGTGGCTTCTACACTTACACTCAAAACACTTCACTTTGTGTCCGAGAATAAACGGAAAAAAACAGCGTTGCCGTGTAAAAGTTGTAGAGGGAAAGGGTTTTATATATGCAAGTTATGCAGCGGGAATTCAACCATAGCTTGGTCTCCAATGTATGATCCTGTTGCAATTAACCCATGTGTTTGTCCTACTTGCGATGGAAACAGGGTACAACGTTGTCTGAATTGTCTGGGAAAAGGCTATGACTAA

Protein sequence

>MsG0880046736.01.T01

MNPIPRRFPTWITGAFVILGGIATLNVASTLTLKTLHFVSENKRKKTALPCKSCRGKGFYICKLCSGNSTIAWSPMYDPVAINPCVCPTCDGNRVQRCLNCLGKGYD*