Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001030.01.T01 | KEH40444.1 | 77.778 | 90 | 17 | 1 | 1 | 90 | 88 | 174 | 1.13E-40 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001030.01.T01 | A0A072VEL6 | 77.778 | 90 | 17 | 1 | 1 | 90 | 88 | 174 | 5.39e-41 | 145 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048928.01 | MsG0180001030.01 | 0.810452 | 1.144430e-50 | 6.900883e-48 |
| MsG0080048952.01 | MsG0180001030.01 | 0.801204 | 1.005538e-48 | 4.773821e-46 |
| MsG0180000604.01 | MsG0180001030.01 | 0.807372 | 5.219147e-50 | 2.901989e-47 |
| MsG0180000680.01 | MsG0180001030.01 | 0.803958 | 2.719071e-49 | 1.384822e-46 |
| MsG0180000741.01 | MsG0180001030.01 | 0.805861 | 1.087911e-49 | 5.818855e-47 |
| MsG0180000797.01 | MsG0180001030.01 | 0.808221 | 3.444650e-50 | 1.958432e-47 |
| MsG0180000821.01 | MsG0180001030.01 | 0.801156 | 1.028254e-48 | 4.875949e-46 |
| MsG0180001030.01 | MsG0180001566.01 | 0.812684 | 3.745511e-51 | 2.397001e-48 |
| MsG0180001030.01 | MsG0180002071.01 | 0.803257 | 3.800251e-49 | 1.900830e-46 |
| MsG0180001030.01 | MsG0180002075.01 | 0.803535 | 3.328648e-49 | 1.676962e-46 |
| MsG0180001030.01 | MsG0180003181.01 | 0.814410 | 1.562474e-51 | 1.047696e-48 |
| MsG0180001030.01 | MsG0180004272.01 | 0.818640 | 1.764380e-52 | 1.327999e-49 |
| MsG0180001030.01 | MsG0180004388.01 | 0.813096 | 3.042332e-51 | 1.968235e-48 |
| MsG0180001030.01 | MsG0280006367.01 | 0.815973 | 7.023776e-52 | 4.914100e-49 |
| MsG0180001030.01 | MsG0280007764.01 | 0.810559 | 1.085181e-50 | 6.562441e-48 |
| MsG0180001030.01 | MsG0280008004.01 | 0.822530 | 2.255151e-53 | 1.892600e-50 |
| MsG0180001030.01 | MsG0280011201.01 | 0.801033 | 1.089578e-48 | 5.150895e-46 |
| MsG0180001030.01 | MsG0380015980.01 | 0.803413 | 3.528441e-49 | 1.771955e-46 |
| MsG0180001030.01 | MsG0380016905.01 | 0.810716 | 1.003668e-50 | 6.095262e-48 |
| MsG0180001030.01 | MsG0380017335.01 | 0.818113 | 2.321568e-52 | 1.722285e-49 |
| MsG0180001030.01 | MsG0380017631.01 | 0.806455 | 8.157234e-50 | 4.429577e-47 |
| MsG0180001030.01 | MsG0480018606.01 | 0.818044 | 2.407211e-52 | 1.782327e-49 |
| MsG0180001030.01 | MsG0480018640.01 | 0.820039 | 8.466873e-53 | 6.626198e-50 |
| MsG0180001030.01 | MsG0480020242.01 | 0.805462 | 1.319322e-49 | 6.984486e-47 |
| MsG0180001030.01 | MsG0480020331.01 | 0.800565 | 1.357531e-48 | 6.341435e-46 |
| MsG0180001030.01 | MsG0480022094.01 | 0.802563 | 5.286024e-49 | 2.597543e-46 |
| MsG0180001030.01 | MsG0480022115.01 | 0.826753 | 2.281330e-54 | 2.158804e-51 |
| MsG0180001030.01 | MsG0480022597.01 | 0.800080 | 1.704202e-48 | 7.863568e-46 |
| MsG0180001030.01 | MsG0480023377.01 | 0.818259 | 2.152107e-52 | 1.602900e-49 |
| MsG0180001030.01 | MsG0580024157.01 | 0.830854 | 2.323034e-55 | 2.477643e-52 |
| MsG0180001030.01 | MsG0580029628.01 | 0.801235 | 9.909369e-49 | 4.708262e-46 |
| MsG0180001030.01 | MsG0580030144.01 | 0.805308 | 1.421019e-49 | 7.492875e-47 |
| MsG0180001030.01 | MsG0580030204.01 | 0.800214 | 1.600945e-48 | 7.412172e-46 |
| MsG0180001030.01 | MsG0680034655.01 | 0.808951 | 2.405803e-50 | 1.394201e-47 |
| MsG0180001030.01 | MsG0780036609.01 | 0.801903 | 7.227196e-49 | 3.492396e-46 |
| MsG0180001030.01 | MsG0780038443.01 | 0.811069 | 8.418347e-51 | 5.160606e-48 |
| MsG0180001030.01 | MsG0780038495.01 | 0.801820 | 7.516025e-49 | 3.624293e-46 |
| MsG0180001030.01 | MsG0780038937.01 | 0.804566 | 2.030996e-49 | 1.050647e-46 |
| MsG0180001030.01 | MsG0780039717.01 | 0.804616 | 1.982821e-49 | 1.027037e-46 |
| MsG0180001030.01 | MsG0780041300.01 | 0.808126 | 3.609883e-50 | 2.047181e-47 |
| MsG0180001030.01 | MsG0780041390.01 | 0.810571 | 1.079045e-50 | 6.527229e-48 |
| MsG0180001030.01 | MsG0880043252.01 | 0.803104 | 4.087536e-49 | 2.036440e-46 |
| MsG0180001030.01 | MsG0880045583.01 | 0.812551 | 4.004214e-51 | 2.553654e-48 |
| MsG0180001030.01 | MsG0880045756.01 | 0.819179 | 1.330113e-52 | 1.016218e-49 |
| MsG0180001030.01 | MsG0880046027.01 | 0.805193 | 1.502139e-49 | 7.897195e-47 |
| MsG0180001030.01 | MsG0880046921.01 | 0.800222 | 1.595079e-48 | 7.386395e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001030.01.T01 | MTR_1g029000 | 77.778 | 90 | 17 | 1 | 1 | 90 | 88 | 174 | 1.37e-44 | 145 |
| MsG0180001030.01.T01 | MTR_1g029000 | 77.778 | 90 | 17 | 1 | 1 | 90 | 88 | 174 | 1.92e-43 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001030.01.T01 | AT1G21370 | 75.385 | 65 | 16 | 0 | 4 | 68 | 91 | 155 | 2.18e-30 | 111 |
| MsG0180001030.01.T01 | AT1G21370 | 75.385 | 65 | 16 | 0 | 4 | 68 | 91 | 155 | 2.18e-30 | 111 |
Find 29 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGAGGATTCGTGGTATT+TGG | 0.173898 | 1:+14837632 | MsG0180001030.01.T01:CDS |
| GTTCTCGAAGCTGAGGAATT+TGG | 0.272054 | 1:+14837557 | MsG0180001030.01.T01:CDS |
| AGGAATTTGGTTATGGTTGC+TGG | 0.293711 | 1:+14837570 | MsG0180001030.01.T01:CDS |
| GGAGTGAGGCGCAGAGTTAT+TGG | 0.328099 | 1:+14837789 | MsG0180001030.01.T01:CDS |
| AAGGAGTTGGATTGTTGATA+AGG | 0.356147 | 1:+14837709 | MsG0180001030.01.T01:CDS |
| GAAGCTGAGGAATTTGGTTA+TGG | 0.375530 | 1:+14837563 | MsG0180001030.01.T01:CDS |
| CAATTACGCGAAAACTGATC+TGG | 0.389611 | 1:+14837518 | MsG0180001030.01.T01:CDS |
| TTGTGAGAAGATTGAGAAAG+AGG | 0.399905 | 1:+14837617 | MsG0180001030.01.T01:CDS |
| GAGTGAGGCGCAGAGTTATT+GGG | 0.409514 | 1:+14837790 | MsG0180001030.01.T01:CDS |
| CGAGGAAAGACGCGGGGCCG+AGG | 0.441720 | 1:+14837768 | MsG0180001030.01.T01:CDS |
| AAGCATATTGAGGAAGGAGT+TGG | 0.482468 | 1:+14837696 | MsG0180001030.01.T01:CDS |
| GATTGTTGATAAGGATGAGA+AGG | 0.487962 | 1:+14837718 | MsG0180001030.01.T01:CDS |
| AGATTGAGAAAGAGGATTCG+TGG | 0.506178 | 1:+14837625 | MsG0180001030.01.T01:CDS |
| GAGAGTTATCAGAAGAATCC+TGG | 0.509383 | 1:+14837654 | MsG0180001030.01.T01:CDS |
| TTATCAGAAGAATCCTGGTC+AGG | 0.520509 | 1:+14837659 | MsG0180001030.01.T01:CDS |
| ATTGTTGATAAGGATGAGAA+GGG | 0.542307 | 1:+14837719 | MsG0180001030.01.T01:CDS |
| TAACTCTGCGCCTCACTCCT+CGG | 0.545516 | 1:-14837785 | None:intergenic |
| AGACGCGGGGCCGAGGAGTG+AGG | 0.561580 | 1:+14837775 | MsG0180001030.01.T01:CDS |
| AATTACGCGAAAACTGATCT+GGG | 0.563506 | 1:+14837519 | MsG0180001030.01.T01:CDS |
| CATTCGATTTATACGAGCAG+TGG | 0.566831 | 1:+14837594 | MsG0180001030.01.T01:CDS |
| TACGTTTGTGAAGCATATTG+AGG | 0.581660 | 1:+14837686 | MsG0180001030.01.T01:CDS |
| ACAAACGTAGCAGCCTGACC+AGG | 0.587052 | 1:-14837672 | None:intergenic |
| TTTGTGAAGCATATTGAGGA+AGG | 0.587362 | 1:+14837690 | MsG0180001030.01.T01:CDS |
| AAATTCCTCAGCTTCGAGAA+CGG | 0.606729 | 1:-14837555 | None:intergenic |
| TGTTTAGTGGTGGTGAGACG+AGG | 0.615572 | 1:+14837750 | MsG0180001030.01.T01:CDS |
| ATTATCCGTTCTCGAAGCTG+AGG | 0.631684 | 1:+14837550 | MsG0180001030.01.T01:CDS |
| GGTGAGACGAGGAAAGACGC+GGG | 0.645521 | 1:+14837761 | MsG0180001030.01.T01:CDS |
| TGGTGAGACGAGGAAAGACG+CGG | 0.648676 | 1:+14837760 | MsG0180001030.01.T01:CDS |
| GTGAGACGAGGAAAGACGCG+GGG | 0.694941 | 1:+14837762 | MsG0180001030.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGTTGATAAGGATGAGAA+GGG | + | Chr1:14837719-14837738 | MsG0180001030.01.T01:CDS | 30.0% |
| AAGGAGTTGGATTGTTGATA+AGG | + | Chr1:14837709-14837728 | MsG0180001030.01.T01:CDS | 35.0% | |
| AATTACGCGAAAACTGATCT+GGG | + | Chr1:14837519-14837538 | MsG0180001030.01.T01:CDS | 35.0% | |
| TACGTTTGTGAAGCATATTG+AGG | + | Chr1:14837686-14837705 | MsG0180001030.01.T01:CDS | 35.0% | |
| TTGTGAGAAGATTGAGAAAG+AGG | + | Chr1:14837617-14837636 | MsG0180001030.01.T01:CDS | 35.0% | |
| TTTGTGAAGCATATTGAGGA+AGG | + | Chr1:14837690-14837709 | MsG0180001030.01.T01:CDS | 35.0% | |
| ! | GATTGTTGATAAGGATGAGA+AGG | + | Chr1:14837718-14837737 | MsG0180001030.01.T01:CDS | 35.0% |
| !! | AAGGGTTTGCTTTTGTTTAG+TGG | + | Chr1:14837737-14837756 | MsG0180001030.01.T01:CDS | 35.0% |
| !! | GCTGATTTTAAAGGATGGTT+TGG | + | Chr1:14837818-14837837 | MsG0180001030.01.T01:CDS | 35.0% |
| !!! | CTGTTGCTGATTTTAAAGGA+TGG | + | Chr1:14837813-14837832 | MsG0180001030.01.T01:CDS | 35.0% |
| AAATTCCTCAGCTTCGAGAA+CGG | - | Chr1:14837558-14837577 | None:intergenic | 40.0% | |
| AAGCATATTGAGGAAGGAGT+TGG | + | Chr1:14837696-14837715 | MsG0180001030.01.T01:CDS | 40.0% | |
| AGATTGAGAAAGAGGATTCG+TGG | + | Chr1:14837625-14837644 | MsG0180001030.01.T01:CDS | 40.0% | |
| AGGAATTTGGTTATGGTTGC+TGG | + | Chr1:14837570-14837589 | MsG0180001030.01.T01:CDS | 40.0% | |
| CAATTACGCGAAAACTGATC+TGG | + | Chr1:14837518-14837537 | MsG0180001030.01.T01:CDS | 40.0% | |
| CATTCGATTTATACGAGCAG+TGG | + | Chr1:14837594-14837613 | MsG0180001030.01.T01:CDS | 40.0% | |
| GAAAGAGGATTCGTGGTATT+TGG | + | Chr1:14837632-14837651 | MsG0180001030.01.T01:CDS | 40.0% | |
| GAAGCTGAGGAATTTGGTTA+TGG | + | Chr1:14837563-14837582 | MsG0180001030.01.T01:CDS | 40.0% | |
| GAGAGTTATCAGAAGAATCC+TGG | + | Chr1:14837654-14837673 | MsG0180001030.01.T01:CDS | 40.0% | |
| TTATCAGAAGAATCCTGGTC+AGG | + | Chr1:14837659-14837678 | MsG0180001030.01.T01:CDS | 40.0% | |
| !! | GGTTTGCTTTTGTTTAGTGG+TGG | + | Chr1:14837740-14837759 | MsG0180001030.01.T01:CDS | 40.0% |
| !!! | TGGGCTGTTGCTGATTTTAA+AGG | + | Chr1:14837809-14837828 | MsG0180001030.01.T01:CDS | 40.0% |
| ATTATCCGTTCTCGAAGCTG+AGG | + | Chr1:14837550-14837569 | MsG0180001030.01.T01:CDS | 45.0% | |
| GTTCTCGAAGCTGAGGAATT+TGG | + | Chr1:14837557-14837576 | MsG0180001030.01.T01:CDS | 45.0% | |
| GAGTGAGGCGCAGAGTTATT+GGG | + | Chr1:14837790-14837809 | MsG0180001030.01.T01:CDS | 50.0% | |
| ! | TGTTTAGTGGTGGTGAGACG+AGG | + | Chr1:14837750-14837769 | MsG0180001030.01.T01:CDS | 50.0% |
| ACAAACGTAGCAGCCTGACC+AGG | - | Chr1:14837675-14837694 | None:intergenic | 55.0% | |
| GGAGTGAGGCGCAGAGTTAT+TGG | + | Chr1:14837789-14837808 | MsG0180001030.01.T01:CDS | 55.0% | |
| TAACTCTGCGCCTCACTCCT+CGG | - | Chr1:14837788-14837807 | None:intergenic | 55.0% | |
| TGGTGAGACGAGGAAAGACG+CGG | + | Chr1:14837760-14837779 | MsG0180001030.01.T01:CDS | 55.0% | |
| GGTGAGACGAGGAAAGACGC+GGG | + | Chr1:14837761-14837780 | MsG0180001030.01.T01:CDS | 60.0% | |
| GTGAGACGAGGAAAGACGCG+GGG | + | Chr1:14837762-14837781 | MsG0180001030.01.T01:CDS | 60.0% | |
| AGACGCGGGGCCGAGGAGTG+AGG | + | Chr1:14837775-14837794 | MsG0180001030.01.T01:CDS | 75.0% | |
| CGAGGAAAGACGCGGGGCCG+AGG | + | Chr1:14837768-14837787 | MsG0180001030.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 14837513 | 14837863 | 14837513 | ID=MsG0180001030.01;Name=MsG0180001030.01 |
| Chr1 | mRNA | 14837513 | 14837863 | 14837513 | ID=MsG0180001030.01.T01;Parent=MsG0180001030.01;Name=MsG0180001030.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|116 |
| Chr1 | exon | 14837513 | 14837863 | 14837513 | ID=MsG0180001030.01.T01:exon:30891;Parent=MsG0180001030.01.T01 |
| Chr1 | CDS | 14837513 | 14837863 | 14837513 | ID=MsG0180001030.01.T01:cds;Parent=MsG0180001030.01.T01 |
| Gene Sequence |
| Protein sequence |
