AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045756.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045756.01.T01 MTR_8g078100 76.316 228 13 4 1 187 18 245 1.20e-113 328
MsG0880045756.01.T01 MTR_5g019430 57.391 230 55 3 1 187 21 250 1.69e-79 241
MsG0880045756.01.T01 MTR_4g069160 48.804 209 68 4 7 176 25 233 1.90e-56 182
MsG0880045756.01.T01 MTR_1g034480 48.558 208 68 4 8 176 22 229 3.53e-52 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045756.01.T01 AT2G18630 46.479 213 74 3 4 176 27 239 3.39e-51 169
MsG0880045756.01.T01 AT2G18630 46.479 213 74 3 4 176 27 239 3.71e-51 169
MsG0880045756.01.T01 AT4G34320 46.190 210 73 4 7 176 19 228 6.78e-50 165
MsG0880045756.01.T01 AT4G34320 46.190 210 73 4 7 176 19 228 6.78e-50 165
MsG0880045756.01.T01 AT4G34330 45.813 203 83 3 7 182 17 219 6.88e-50 165
MsG0880045756.01.T01 AT5G66675 43.578 218 77 4 5 176 36 253 1.13e-49 166
MsG0880045756.01.T01 AT5G66675 43.578 218 77 4 5 176 36 253 1.13e-49 166
MsG0880045756.01.T01 AT5G66675 43.578 218 77 4 5 176 36 253 1.13e-49 166
MsG0880045756.01.T01 AT5G66675 43.578 218 77 4 5 176 36 253 1.13e-49 166
MsG0880045756.01.T01 AT5G66660 38.696 230 95 3 4 187 35 264 1.44e-32 120
MsG0880045756.01.T01 AT5G66670 50.000 86 43 0 102 187 178 263 3.50e-17 78.6
MsG0880045756.01.T01 AT5G66670 50.704 71 35 0 4 74 35 105 3.82e-14 70.1
MsG0880045756.01.T01 AT5G66670 50.000 86 43 0 102 187 178 263 3.50e-17 78.6
MsG0880045756.01.T01 AT5G66670 50.704 71 35 0 4 74 35 105 3.82e-14 70.1
MsG0880045756.01.T01 AT5G66670 50.000 86 43 0 102 187 178 263 3.50e-17 78.6
MsG0880045756.01.T01 AT5G66670 50.704 71 35 0 4 74 35 105 3.82e-14 70.1
MsG0880045756.01.T01 AT5G66670 50.000 86 43 0 102 187 178 263 3.50e-17 78.6
MsG0880045756.01.T01 AT5G66670 50.704 71 35 0 4 74 35 105 3.82e-14 70.1
MsG0880045756.01.T01 AT3G28290 26.106 226 113 7 7 178 11 236 4.53e-15 72.4
MsG0880045756.01.T01 AT3G28300 26.106 226 113 7 7 178 11 236 4.53e-15 72.4

Find 31 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAGTTGTTAAAGTTATT+TGG 0.146302 8:+63841416 MsG0880045756.01.T01:CDS
AAATAAAACTCCTCCGTAAA+AGG 0.159235 8:-63841682 None:intergenic
TCATCAAAACTTTGCAAGTT+TGG 0.189922 8:-63841277 None:intergenic
TTGGATTGCAAGAGGATATA+TGG 0.215547 8:+63841435 MsG0880045756.01.T01:CDS
TTTGAAGAAGAGGTGCAAAA+TGG 0.284763 8:+63841595 MsG0880045756.01.T01:intron
TTGCTACTGGTGTTGAAGTT+AGG 0.293911 8:+63841338 MsG0880045756.01.T01:CDS
GTTATTAGTTCTCTTGCTAC+TGG 0.384834 8:+63841325 MsG0880045756.01.T01:CDS
CCGCAGCCAAAGCTGTTAAA+CGG 0.411019 8:-63841890 None:intergenic
TGGATTGCAAGAGGATATAT+GGG 0.411552 8:+63841436 MsG0880045756.01.T01:CDS
CGCAGCCAAAGCTGTTAAAC+GGG 0.415820 8:-63841889 None:intergenic
GAGGTGCAAAATGGGCTTGA+AGG 0.435928 8:+63841604 MsG0880045756.01.T01:CDS
TTGAAGAAGAGGTGCAAAAT+GGG 0.437385 8:+63841596 MsG0880045756.01.T01:intron
AAGAGAACTAATAACCCTAT+TGG 0.447555 8:-63841317 None:intergenic
GTATTAAAGAGCATACCAAT+AGG 0.457807 8:+63841302 MsG0880045756.01.T01:CDS
AGGTGCAAAATGGGCTTGAA+GGG 0.480313 8:+63841605 MsG0880045756.01.T01:CDS
TGAAGACATTGCAAGAGCTT+AGG 0.500716 8:+63841638 MsG0880045756.01.T01:CDS
TCAATCGGTTTATACGCAGC+AGG 0.509528 8:+63841711 MsG0880045756.01.T01:CDS
TATTAAAGAGCATACCAATA+GGG 0.511363 8:+63841303 MsG0880045756.01.T01:CDS
AATAAAACTCCTCCGTAAAA+GGG 0.512251 8:-63841681 None:intergenic
ATATGAAGCTGCATGTATCA+AGG 0.527102 8:+63841252 MsG0880045756.01.T01:CDS
AAACAAGGATTTGTTCGCGT+TGG 0.546843 8:+63841460 MsG0880045756.01.T01:CDS
AAAGTTATTTGGATTGCAAG+AGG 0.549185 8:+63841427 MsG0880045756.01.T01:CDS
AGTTGAAATCACTCAAGTCA+TGG 0.549628 8:+63841782 MsG0880045756.01.T01:CDS
AATCACTCAAGTCATGGAAG+AGG 0.591019 8:+63841788 MsG0880045756.01.T01:CDS
GATTTGCAATTTCTTCAACA+TGG 0.599579 8:-63841737 None:intergenic
ATCACTCAAGTCATGGAAGA+GGG 0.610181 8:+63841789 MsG0880045756.01.T01:CDS
GGTGCAAAATGGGCTTGAAG+GGG 0.611042 8:+63841606 MsG0880045756.01.T01:CDS
AGAGGATATATGGGGAAACA+AGG 0.620871 8:+63841445 MsG0880045756.01.T01:CDS
AGCCAAAGCTGTTAAACGGG+CGG 0.623829 8:-63841886 None:intergenic
GTGCAAAATGGGCTTGAAGG+GGG 0.641754 8:+63841607 MsG0880045756.01.T01:CDS
GGATTGCAAGAGGATATATG+GGG 0.705897 8:+63841437 MsG0880045756.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAGTTGTTAAAGTTATT+TGG + Chr8:63841416-63841435 MsG0880045756.01.T01:CDS 20.0%
!!! GTTTTATTTGTTGTTTCAAT+CGG + Chr8:63841696-63841715 MsG0880045756.01.T01:CDS 20.0%
! TGATTACTTATTTGAAGAAG+AGG + Chr8:63841585-63841604 MsG0880045756.01.T01:intron 25.0%
!! TATTAAAGAGCATACCAATA+GGG + Chr8:63841303-63841322 MsG0880045756.01.T01:CDS 25.0%
AAAGTTATTTGGATTGCAAG+AGG + Chr8:63841427-63841446 MsG0880045756.01.T01:CDS 30.0%
AAATAAAACTCCTCCGTAAA+AGG - Chr8:63841685-63841704 None:intergenic 30.0%
AAGAGAACTAATAACCCTAT+TGG - Chr8:63841320-63841339 None:intergenic 30.0%
AATAAAACTCCTCCGTAAAA+GGG - Chr8:63841684-63841703 None:intergenic 30.0%
GAATCAAGTGATTGTTAAGT+CGG + Chr8:63841562-63841581 MsG0880045756.01.T01:intron 30.0%
GATTTGCAATTTCTTCAACA+TGG - Chr8:63841740-63841759 None:intergenic 30.0%
TCATCAAAACTTTGCAAGTT+TGG - Chr8:63841280-63841299 None:intergenic 30.0%
! GTATTAAAGAGCATACCAAT+AGG + Chr8:63841302-63841321 MsG0880045756.01.T01:CDS 30.0%
!! TTAGGAATTTTAAGGATGCT+GGG + Chr8:63841656-63841675 MsG0880045756.01.T01:CDS 30.0%
!!! GGTGTCAAATGCAATTTTTT+TGG + Chr8:63841810-63841829 MsG0880045756.01.T01:CDS 30.0%
AATTACAAAACTCCAACGTC+TGG - Chr8:63841511-63841530 None:intergenic 35.0%
AGTTGAAATCACTCAAGTCA+TGG + Chr8:63841782-63841801 MsG0880045756.01.T01:CDS 35.0%
ATATGAAGCTGCATGTATCA+AGG + Chr8:63841252-63841271 MsG0880045756.01.T01:CDS 35.0%
CTCTTGAGAAATGTTTGAGA+CGG + Chr8:63841531-63841550 MsG0880045756.01.T01:intron 35.0%
TGGATTGCAAGAGGATATAT+GGG + Chr8:63841436-63841455 MsG0880045756.01.T01:CDS 35.0%
TTGGATTGCAAGAGGATATA+TGG + Chr8:63841435-63841454 MsG0880045756.01.T01:CDS 35.0%
! GCAAGAGCTTAGGAATTTTA+AGG + Chr8:63841648-63841667 MsG0880045756.01.T01:CDS 35.0%
! GTTATTAGTTCTCTTGCTAC+TGG + Chr8:63841325-63841344 MsG0880045756.01.T01:CDS 35.0%
! TCTTGAGAAATGTTTGAGAC+GGG + Chr8:63841532-63841551 MsG0880045756.01.T01:intron 35.0%
! TTGAAGAAGAGGTGCAAAAT+GGG + Chr8:63841596-63841615 MsG0880045756.01.T01:intron 35.0%
! TTTGAAGAAGAGGTGCAAAA+TGG + Chr8:63841595-63841614 MsG0880045756.01.T01:intron 35.0%
!! CTTAGGAATTTTAAGGATGC+TGG + Chr8:63841655-63841674 MsG0880045756.01.T01:CDS 35.0%
!! TAGGAATTTTAAGGATGCTG+GGG + Chr8:63841657-63841676 MsG0880045756.01.T01:CDS 35.0%
!!! TTTTTTGGCTGCGTTTGTTT+CGG + Chr8:63841825-63841844 MsG0880045756.01.T01:CDS 35.0%
AATCACTCAAGTCATGGAAG+AGG + Chr8:63841788-63841807 MsG0880045756.01.T01:CDS 40.0%
AGAGGATATATGGGGAAACA+AGG + Chr8:63841445-63841464 MsG0880045756.01.T01:CDS 40.0%
ATCACTCAAGTCATGGAAGA+GGG + Chr8:63841789-63841808 MsG0880045756.01.T01:CDS 40.0%
GGATTGCAAGAGGATATATG+GGG + Chr8:63841437-63841456 MsG0880045756.01.T01:CDS 40.0%
TGAAGACATTGCAAGAGCTT+AGG + Chr8:63841638-63841657 MsG0880045756.01.T01:CDS 40.0%
TGATAATAGTCTCCAGACGT+TGG + Chr8:63841496-63841515 MsG0880045756.01.T01:intron 40.0%
! AAACAAGGATTTGTTCGCGT+TGG + Chr8:63841460-63841479 MsG0880045756.01.T01:CDS 40.0%
! TTGCTACTGGTGTTGAAGTT+AGG + Chr8:63841338-63841357 MsG0880045756.01.T01:CDS 40.0%
!!! TTCGGTGTTGATTTTCTCTG+TGG + Chr8:63841843-63841862 MsG0880045756.01.T01:CDS 40.0%
! AGGTGCAAAATGGGCTTGAA+GGG + Chr8:63841605-63841624 MsG0880045756.01.T01:CDS 45.0%
! TCAATCGGTTTATACGCAGC+AGG + Chr8:63841711-63841730 MsG0880045756.01.T01:CDS 45.0%
! GAGGTGCAAAATGGGCTTGA+AGG + Chr8:63841604-63841623 MsG0880045756.01.T01:CDS 50.0%
! GGTGCAAAATGGGCTTGAAG+GGG + Chr8:63841606-63841625 MsG0880045756.01.T01:CDS 50.0%
! GTGCAAAATGGGCTTGAAGG+GGG + Chr8:63841607-63841626 MsG0880045756.01.T01:CDS 50.0%
! GGATGCTGGGGACCCTTTTA+CGG + Chr8:63841669-63841688 MsG0880045756.01.T01:CDS 55.0%
! TGCTGGGGACCCTTTTACGG+AGG + Chr8:63841672-63841691 MsG0880045756.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 63841214 63841897 63841214 ID=MsG0880045756.01;Name=MsG0880045756.01
Chr8 mRNA 63841214 63841897 63841214 ID=MsG0880045756.01.T01;Parent=MsG0880045756.01;Name=MsG0880045756.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|187
Chr8 exon 63841214 63841481 63841214 ID=MsG0880045756.01.T01:exon:3140;Parent=MsG0880045756.01.T01
Chr8 exon 63841602 63841897 63841602 ID=MsG0880045756.01.T01:exon:3141;Parent=MsG0880045756.01.T01
Chr8 CDS 63841214 63841481 63841214 ID=MsG0880045756.01.T01:cds;Parent=MsG0880045756.01.T01
Chr8 CDS 63841602 63841897 63841602 ID=MsG0880045756.01.T01:cds;Parent=MsG0880045756.01.T01
Gene Sequence

>MsG0880045756.01.T01

ATGGGTACTCATTCTGTGTATGCAGCTGATTTAAGCTCATATGAAGCTGCATGTATCAAGGATCCAAACTTGCAAAGTTTTGATGAGAGTATTAAAGAGCATACCAATAGGGTTATTAGTTCTCTTGCTACTGGTGTTGAAGTTAGGTCTTTATCTTTCGATTCGCTGCGAGAAGTTACTGATAGTTTGCTTGAGATGAATCATGAAGTTGTTAAAGTTATTTGGATTGCAAGAGGATATATGGGGAAACAAGGATTTGTTCGCGTTGAAGAGGTGCAAAATGGGCTTGAAGGGGGAACTTGTGTGAAGACATTGCAAGAGCTTAGGAATTTTAAGGATGCTGGGGACCCTTTTACGGAGGAGTTTTATTTGTTGTTTCAATCGGTTTATACGCAGCAGGCAACCATGTTGAAGAAATTGCAAATCAGAAAGAGAAAGCTTGATAAGAAGTTGAAATCACTCAAGTCATGGAAGAGGGTGTCAAATGCAATTTTTTTGGCTGCGTTTGTTTCGGTGTTGATTTTCTCTGTGGTTGCAGCTTCTATTGCTGCTCCGCCCGTTTAA

Protein sequence

>MsG0880045756.01.T01

MGTHSVYAADLSSYEAACIKDPNLQSFDESIKEHTNRVISSLATGVEVRSLSFDSLREVTDSLLEMNHEVVKVIWIARGYMGKQGFVRVEEVQNGLEGGTCVKTLQELRNFKDAGDPFTEEFYLLFQSVYTQQATMLKKLQIRKRKLDKKLKSLKSWKRVSNAIFLAAFVSVLIFSVVAASIAAPPV*