Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004748.01.T01 | XP_013469145.1 | 93.333 | 60 | 3 | 1 | 1 | 59 | 117 | 176 | 1.69E-31 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004748.01.T01 | Q9M1R4 | 66.667 | 54 | 16 | 2 | 1 | 53 | 116 | 168 | 1.56E-16 | 71.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004748.01.T01 | A0A072VP23 | 93.333 | 60 | 3 | 1 | 1 | 59 | 117 | 176 | 8.06e-32 | 117 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180004748.01.T01 | TR | AUX/IAA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0180004748.01 | 0.821881 | 3.189905e-53 | 2.627948e-50 |
| MsG0080048952.01 | MsG0180004748.01 | 0.805447 | 1.329032e-49 | 7.032932e-47 |
| MsG0180000295.01 | MsG0180004748.01 | 0.810942 | 8.970601e-51 | 5.480990e-48 |
| MsG0180000324.01 | MsG0180004748.01 | 0.805733 | 1.157360e-49 | 6.170194e-47 |
| MsG0180001173.01 | MsG0180004748.01 | 0.814136 | 1.796901e-51 | 1.195872e-48 |
| MsG0180001237.01 | MsG0180004748.01 | 0.807141 | 5.844051e-50 | 3.229849e-47 |
| MsG0180001566.01 | MsG0180004748.01 | 0.828413 | 9.114593e-55 | 9.053513e-52 |
| MsG0180002075.01 | MsG0180004748.01 | 0.804416 | 2.183204e-49 | 1.124996e-46 |
| MsG0180003822.01 | MsG0180004748.01 | 0.803827 | 2.895414e-49 | 1.469622e-46 |
| MsG0180004272.01 | MsG0180004748.01 | 0.812414 | 4.291258e-51 | 2.726832e-48 |
| MsG0180004748.01 | MsG0180005233.01 | 0.806516 | 7.921067e-50 | 4.307864e-47 |
| MsG0180004748.01 | MsG0180005443.01 | 0.832996 | 6.873220e-56 | 7.811812e-53 |
| MsG0180004748.01 | MsG0180005451.01 | 0.822638 | 2.128151e-53 | 1.791568e-50 |
| MsG0180004748.01 | MsG0180005493.01 | 0.805035 | 1.621060e-49 | 8.487910e-47 |
| MsG0180004748.01 | MsG0280007491.01 | 0.814622 | 1.402940e-51 | 9.460365e-49 |
| MsG0180004748.01 | MsG0280007609.01 | 0.802568 | 5.275562e-49 | 2.592679e-46 |
| MsG0180004748.01 | MsG0280007764.01 | 0.832348 | 9.948791e-56 | 1.108977e-52 |
| MsG0180004748.01 | MsG0280008004.01 | 0.815308 | 9.879375e-52 | 6.787081e-49 |
| MsG0180004748.01 | MsG0380013549.01 | 0.822892 | 1.857051e-53 | 1.574558e-50 |
| MsG0180004748.01 | MsG0380015893.01 | 0.807469 | 4.978754e-50 | 2.775438e-47 |
| MsG0180004748.01 | MsG0380016765.01 | 0.815426 | 9.302032e-52 | 6.411194e-49 |
| MsG0180004748.01 | MsG0380016905.01 | 0.807920 | 3.992996e-50 | 2.252066e-47 |
| MsG0180004748.01 | MsG0380016971.01 | 0.801966 | 7.016463e-49 | 3.395780e-46 |
| MsG0180004748.01 | MsG0380016994.01 | 0.806428 | 8.264614e-50 | 4.484735e-47 |
| MsG0180004748.01 | MsG0380017375.01 | 0.816615 | 5.047356e-52 | 3.593811e-49 |
| MsG0180004748.01 | MsG0380017431.01 | 0.805414 | 1.350694e-49 | 7.141435e-47 |
| MsG0180004748.01 | MsG0480018640.01 | 0.809817 | 1.569025e-50 | 9.305243e-48 |
| MsG0180004748.01 | MsG0480020242.01 | 0.815177 | 1.056384e-51 | 7.230900e-49 |
| MsG0180004748.01 | MsG0480020591.01 | 0.812537 | 4.034380e-51 | 2.571922e-48 |
| MsG0180004748.01 | MsG0480022094.01 | 0.821672 | 3.565524e-53 | 2.919891e-50 |
| MsG0180004748.01 | MsG0480022115.01 | 0.828532 | 8.531880e-55 | 8.503705e-52 |
| MsG0180004748.01 | MsG0480022228.01 | 0.832501 | 9.116976e-56 | 1.020956e-52 |
| MsG0180004748.01 | MsG0580024157.01 | 0.805533 | 1.274826e-49 | 6.760805e-47 |
| MsG0180004748.01 | MsG0580024618.01 | 0.809586 | 1.758365e-50 | 1.036519e-47 |
| MsG0180004748.01 | MsG0580025352.01 | 0.806040 | 9.976669e-50 | 5.360448e-47 |
| MsG0180004748.01 | MsG0780038443.01 | 0.822136 | 2.783816e-53 | 2.310078e-50 |
| MsG0180004748.01 | MsG0780040688.01 | 0.804471 | 2.126564e-49 | 1.097363e-46 |
| MsG0180004748.01 | MsG0780041390.01 | 0.830439 | 2.934924e-55 | 3.092166e-52 |
| MsG0180004748.01 | MsG0880042760.01 | 0.810122 | 1.348758e-50 | 8.062979e-48 |
| MsG0180004748.01 | MsG0880042829.01 | 0.808985 | 2.365807e-50 | 1.372291e-47 |
| MsG0180004748.01 | MsG0880042939.01 | 0.801227 | 9.947322e-49 | 4.725238e-46 |
| MsG0180004748.01 | MsG0880043252.01 | 0.806528 | 7.872473e-50 | 4.282789e-47 |
| MsG0180004748.01 | MsG0880044565.01 | 0.815728 | 7.969156e-52 | 5.538513e-49 |
| MsG0180004748.01 | MsG0880046027.01 | 0.807831 | 4.170628e-50 | 2.346845e-47 |
| MsG0180004748.01 | MsG0880047192.01 | 0.814192 | 1.746445e-51 | 1.164036e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004748.01.T01 | MTR_1g085750 | 93.333 | 60 | 3 | 1 | 1 | 59 | 117 | 176 | 2.04e-35 | 117 |
| MsG0180004748.01.T01 | MTR_7g110790 | 86.441 | 59 | 7 | 1 | 1 | 58 | 102 | 160 | 2.08e-32 | 109 |
| MsG0180004748.01.T01 | MTR_1g085750 | 95.122 | 41 | 1 | 1 | 1 | 40 | 117 | 157 | 2.50e-22 | 83.6 |
| MsG0180004748.01.T01 | MTR_4g124300 | 64.912 | 57 | 18 | 2 | 1 | 56 | 128 | 183 | 6.47e-16 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004748.01.T01 | AT3G62100 | 66.667 | 54 | 16 | 2 | 1 | 53 | 116 | 168 | 1.59e-17 | 71.6 |
| MsG0180004748.01.T01 | AT2G46990 | 60.714 | 56 | 20 | 2 | 1 | 55 | 118 | 172 | 1.39e-16 | 69.3 |
| MsG0180004748.01.T01 | AT3G17600 | 50.000 | 58 | 23 | 2 | 1 | 57 | 106 | 158 | 1.43e-13 | 61.2 |
Find 12 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGAAGGAGACTTGATTA+TGG | 0.334521 | 1:+82091881 | MsG0180004748.01.T01:CDS |
| ATCACAAGGTTTGATGCATT+CGG | 0.368816 | 1:+82092445 | MsG0180004748.01.T01:CDS |
| TCACAAGGTTTGATGCATTC+GGG | 0.382190 | 1:+82092446 | MsG0180004748.01.T01:CDS |
| GCAGAAGGAGACTTGATTAT+GGG | 0.410619 | 1:+82091882 | MsG0180004748.01.T01:CDS |
| GGAGTGGTAATTACATAGAA+TGG | 0.466849 | 1:-82091716 | None:intergenic |
| TGTTAAGTAAATTACTCCCA+AGG | 0.553170 | 1:-82091912 | None:intergenic |
| GTTAAGTAAATTACTCCCAA+GGG | 0.585104 | 1:-82091911 | None:intergenic |
| TGTTCTTATCATCTGTGAAG+AGG | 0.588185 | 1:+82092416 | MsG0180004748.01.T01:CDS |
| CAGAAGGAGACTTGATTATG+GGG | 0.588289 | 1:+82091883 | MsG0180004748.01.T01:CDS |
| TTAACTTATGAAGATGCAGA+AGG | 0.627924 | 1:+82091867 | MsG0180004748.01.T01:CDS |
| TGAAGAGGTTGAAGATCACA+AGG | 0.629998 | 1:+82092431 | MsG0180004748.01.T01:CDS |
| AGAAGGAGACTTGATTATGG+GGG | 0.682204 | 1:+82091884 | MsG0180004748.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCAATTAATTTACAAAAT+TGG | - | Chr1:82092370-82092389 | None:intergenic | 10.0% |
| !!! | ATTTTGTAAATTAATTGATT+TGG | + | Chr1:82092370-82092389 | MsG0180004748.01.T01:intron | 10.0% |
| !!! | TTTTTACTTCTTTTTTTTTT+TGG | + | Chr1:82091753-82091772 | MsG0180004748.01.T01:intron | 10.0% |
| !! | AAATTCACTTAGAATTGAAA+TGG | - | Chr1:82091959-82091978 | None:intergenic | 20.0% |
| !! | AAGTAAAAATGCATATATGA+GGG | - | Chr1:82091742-82091761 | None:intergenic | 20.0% |
| !! | AATTCACTTAGAATTGAAAT+GGG | - | Chr1:82091958-82091977 | None:intergenic | 20.0% |
| !! | ATTTCAATTCTAAGTGAATT+TGG | + | Chr1:82091958-82091977 | MsG0180004748.01.T01:intron | 20.0% |
| !!! | ATTGATTTGGTACATTTTTT+TGG | + | Chr1:82092383-82092402 | MsG0180004748.01.T01:intron | 20.0% |
| !!! | ATTTATGTAACTGTGATTTT+CGG | - | Chr1:82092297-82092316 | None:intergenic | 20.0% |
| !!! | TTTTGGTAAAATGAGTAATA+TGG | + | Chr1:82091770-82091789 | MsG0180004748.01.T01:intron | 20.0% |
| ! | AGTAAAAATGCATATATGAG+GGG | - | Chr1:82091741-82091760 | None:intergenic | 25.0% |
| ! | GAAGTAAAAATGCATATATG+AGG | - | Chr1:82091743-82091762 | None:intergenic | 25.0% |
| !! | AAGTGAATTTGGCATAATTT+TGG | + | Chr1:82091969-82091988 | MsG0180004748.01.T01:intron | 25.0% |
| !!! | GATTTTGACACAAGTTTATT+CGG | + | Chr1:82092243-82092262 | MsG0180004748.01.T01:intron | 25.0% |
| GTAAAAATGCATATATGAGG+GGG | - | Chr1:82091740-82091759 | None:intergenic | 30.0% | |
| GTTAAGTAAATTACTCCCAA+GGG | - | Chr1:82091914-82091933 | None:intergenic | 30.0% | |
| TGTTAAGTAAATTACTCCCA+AGG | - | Chr1:82091915-82091934 | None:intergenic | 30.0% | |
| TTAACTTATGAAGATGCAGA+AGG | + | Chr1:82091867-82091886 | MsG0180004748.01.T01:CDS | 30.0% | |
| TTGTGTCAAAATCACACTAA+GGG | - | Chr1:82092236-82092255 | None:intergenic | 30.0% | |
| ATCACAAGGTTTGATGCATT+CGG | + | Chr1:82092445-82092464 | MsG0180004748.01.T01:CDS | 35.0% | |
| CTTGTGTCAAAATCACACTA+AGG | - | Chr1:82092237-82092256 | None:intergenic | 35.0% | |
| GATGATAAGAACATCCTACA+TGG | - | Chr1:82092409-82092428 | None:intergenic | 35.0% | |
| GGAGTGGTAATTACATAGAA+TGG | - | Chr1:82091719-82091738 | None:intergenic | 35.0% | |
| GTTTGAATTGACGATGAGTT+TGG | - | Chr1:82092323-82092342 | None:intergenic | 35.0% | |
| TGTTCTTATCATCTGTGAAG+AGG | + | Chr1:82092416-82092435 | MsG0180004748.01.T01:CDS | 35.0% | |
| ! | TGTAACTGTGATTTTCGGTT+TGG | - | Chr1:82092292-82092311 | None:intergenic | 35.0% |
| !! | TGGCATAATTTTGGTACGTT+AGG | + | Chr1:82091978-82091997 | MsG0180004748.01.T01:intron | 35.0% |
| !!! | GTACATTTTTTTGGCCATGT+AGG | + | Chr1:82092392-82092411 | MsG0180004748.01.T01:intron | 35.0% |
| !!! | GTGACTTTGTTATTTTGCCA+GGG | + | Chr1:82091802-82091821 | MsG0180004748.01.T01:intron | 35.0% |
| !!! | TGACTTTGTTATTTTGCCAG+GGG | + | Chr1:82091803-82091822 | MsG0180004748.01.T01:intron | 35.0% |
| !!! | TGTGACTTTGTTATTTTGCC+AGG | + | Chr1:82091801-82091820 | MsG0180004748.01.T01:intron | 35.0% |
| AGAAGGAGACTTGATTATGG+GGG | + | Chr1:82091884-82091903 | MsG0180004748.01.T01:CDS | 40.0% | |
| CAGAAGGAGACTTGATTATG+GGG | + | Chr1:82091883-82091902 | MsG0180004748.01.T01:CDS | 40.0% | |
| GCAGAAGGAGACTTGATTAT+GGG | + | Chr1:82091882-82091901 | MsG0180004748.01.T01:CDS | 40.0% | |
| TCACAAGGTTTGATGCATTC+GGG | + | Chr1:82092446-82092465 | MsG0180004748.01.T01:CDS | 40.0% | |
| TGAAGAGGTTGAAGATCACA+AGG | + | Chr1:82092431-82092450 | MsG0180004748.01.T01:CDS | 40.0% | |
| TGCAGAAGGAGACTTGATTA+TGG | + | Chr1:82091881-82091900 | MsG0180004748.01.T01:CDS | 40.0% | |
| TTGGTACGTTAGGTGTGTTT+GGG | + | Chr1:82091988-82092007 | MsG0180004748.01.T01:intron | 40.0% | |
| TTTGGTACGTTAGGTGTGTT+TGG | + | Chr1:82091987-82092006 | MsG0180004748.01.T01:intron | 40.0% | |
| ! | AATCACACTAAGGGTGTGTT+TGG | - | Chr1:82092227-82092246 | None:intergenic | 40.0% |
| ! | ATCACACTAAGGGTGTGTTT+GGG | - | Chr1:82092226-82092245 | None:intergenic | 40.0% |
| !!! | ACTTTGTTATTTTGCCAGGG+GGG | + | Chr1:82091805-82091824 | MsG0180004748.01.T01:intron | 40.0% |
| !!! | GACTTTGTTATTTTGCCAGG+GGG | + | Chr1:82091804-82091823 | MsG0180004748.01.T01:intron | 40.0% |
| AATGCATATATGAGGGGGAG+TGG | - | Chr1:82091735-82091754 | None:intergenic | 45.0% | |
| TGTGTTTGGGAAGAGGGTTT+AGG | - | Chr1:82092213-82092232 | None:intergenic | 45.0% | |
| TTTGGGAAGAGGGTTTAGGA+GGG | - | Chr1:82092209-82092228 | None:intergenic | 45.0% | |
| ! | TAAGGGTGTGTTTGGGAAGA+GGG | - | Chr1:82092219-82092238 | None:intergenic | 45.0% |
| AAGAGGGTTTAGGAGGGGAA+GGG | - | Chr1:82092203-82092222 | None:intergenic | 50.0% | |
| ACGTTAGGTGTGTTTGGGAG+AGG | + | Chr1:82091993-82092012 | MsG0180004748.01.T01:intron | 50.0% | |
| CGTTAGGTGTGTTTGGGAGA+GGG | + | Chr1:82091994-82092013 | MsG0180004748.01.T01:intron | 50.0% | |
| GATTATGGGGGATGTCCCTT+GGG | + | Chr1:82091896-82091915 | MsG0180004748.01.T01:CDS | 50.0% | |
| GTGTGTTTGGGAGAGGGTTT+AGG | + | Chr1:82092000-82092019 | MsG0180004748.01.T01:intron | 50.0% | |
| GTTTGGGAAGAGGGTTTAGG+AGG | - | Chr1:82092210-82092229 | None:intergenic | 50.0% | |
| GTTTGGGAGAGGGTTTAGGA+GGG | + | Chr1:82092004-82092023 | MsG0180004748.01.T01:intron | 50.0% | |
| TGATTATGGGGGATGTCCCT+TGG | + | Chr1:82091895-82091914 | MsG0180004748.01.T01:CDS | 50.0% | |
| TGTTTGGGAGAGGGTTTAGG+AGG | + | Chr1:82092003-82092022 | MsG0180004748.01.T01:intron | 50.0% | |
| TTGGGAAGAGGGTTTAGGAG+GGG | - | Chr1:82092208-82092227 | None:intergenic | 50.0% | |
| TTTGGGAGAGGGTTTAGGAG+GGG | + | Chr1:82092005-82092024 | MsG0180004748.01.T01:intron | 50.0% | |
| ! | CTAAGGGTGTGTTTGGGAAG+AGG | - | Chr1:82092220-82092239 | None:intergenic | 50.0% |
| GAAGAGGGTTTAGGAGGGGA+AGG | - | Chr1:82092204-82092223 | None:intergenic | 55.0% | |
| GAGAGGGTTTAGGAGGGGAA+GGG | + | Chr1:82092010-82092029 | MsG0180004748.01.T01:intron | 55.0% | |
| TTTGCCAGGGGGGACTGAAA+TGG | + | Chr1:82091815-82091834 | MsG0180004748.01.T01:intron | 55.0% | |
| AGGGTTTAGGAGGGGAAGGG+AGG | + | Chr1:82092013-82092032 | MsG0180004748.01.T01:intron | 60.0% | |
| AGGGTTTAGGAGGGGAAGGG+AGG | - | Chr1:82092032-82092013 | None:intergenic | 60.0% | |
| CCAGGGGGGACTGAAATGGA+TGG | + | Chr1:82091819-82091838 | MsG0180004748.01.T01:intron | 60.0% | |
| CCATCCATTTCAGTCCCCCC+TGG | - | Chr1:82091822-82091841 | None:intergenic | 60.0% | |
| GGAGAGGGTTTAGGAGGGGA+AGG | + | Chr1:82092009-82092028 | MsG0180004748.01.T01:intron | 60.0% | |
| GGGTTTAGGAGGGGAAGGGA+GGG | + | Chr1:82092014-82092033 | MsG0180004748.01.T01:intron | 60.0% | |
| GGGTTTAGGAGGGGAAGGGA+GGG | - | Chr1:82092033-82092014 | None:intergenic | 60.0% | |
| GGTTTAGGAGGGGAAGGGAG+GGG | + | Chr1:82092015-82092034 | MsG0180004748.01.T01:intron | 60.0% | |
| GGTTTAGGAGGGGAAGGGAG+GGG | - | Chr1:82092034-82092015 | None:intergenic | 60.0% | |
| TAGGAGGGGAAGGGAGGGGA+GGG | + | Chr1:82092019-82092038 | MsG0180004748.01.T01:intron | 65.0% | |
| TTAGGAGGGGAAGGGAGGGG+AGG | + | Chr1:82092018-82092037 | MsG0180004748.01.T01:intron | 65.0% | |
| TTAGGAGGGGAAGGGAGGGG+AGG | - | Chr1:82092037-82092018 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 82091703 | 82092474 | 82091703 | ID=MsG0180004748.01;Name=MsG0180004748.01 |
| Chr1 | mRNA | 82091703 | 82092474 | 82091703 | ID=MsG0180004748.01.T01;Parent=MsG0180004748.01;Name=MsG0180004748.01.T01;_AED=0.50;_eAED=0.52;_QI=0|0|0|1|0|0|3|0|59 |
| Chr1 | exon | 82091703 | 82091724 | 82091703 | ID=MsG0180004748.01.T01:exon:34688;Parent=MsG0180004748.01.T01 |
| Chr1 | exon | 82091823 | 82091919 | 82091823 | ID=MsG0180004748.01.T01:exon:34689;Parent=MsG0180004748.01.T01 |
| Chr1 | exon | 82092414 | 82092474 | 82092414 | ID=MsG0180004748.01.T01:exon:34690;Parent=MsG0180004748.01.T01 |
| Chr1 | CDS | 82091703 | 82091724 | 82091703 | ID=MsG0180004748.01.T01:cds;Parent=MsG0180004748.01.T01 |
| Chr1 | CDS | 82091823 | 82091919 | 82091823 | ID=MsG0180004748.01.T01:cds;Parent=MsG0180004748.01.T01 |
| Chr1 | CDS | 82092414 | 82092474 | 82092414 | ID=MsG0180004748.01.T01:cds;Parent=MsG0180004748.01.T01 |
| Gene Sequence |
| Protein sequence |
