AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009171.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009171.01.T01 MTR_0011s0260 81.818 198 26 5 1 192 1 194 4.94e-90 268
MsG0280009171.01.T01 MTR_0011s0210 81.818 198 26 5 1 192 1 194 1.36e-89 267
MsG0280009171.01.T01 MTR_6g047550 64.141 198 51 4 1 192 1 184 1.77e-76 231
MsG0280009171.01.T01 MTR_6g047570 63.776 196 41 6 1 192 1 170 1.75e-71 220
MsG0280009171.01.T01 MTR_0246s0020 50.230 217 75 8 1 192 1 209 5.32e-51 169
MsG0280009171.01.T01 MTR_0246s0020 50.230 217 75 8 1 192 1 209 7.58e-51 169
MsG0280009171.01.T01 MTR_4g066380 50.481 208 43 6 1 192 1 164 2.88e-50 166
MsG0280009171.01.T01 MTR_4g067010 54.872 195 48 7 1 192 1 158 5.09e-50 165
MsG0280009171.01.T01 MTR_4g066460 51.196 209 42 9 1 192 1 166 1.63e-44 150
MsG0280009171.01.T01 MTR_4g098035 43.564 202 85 9 1 192 1 183 1.14e-33 123
MsG0280009171.01.T01 MTR_0246s0050 41.243 177 76 8 25 192 5 162 3.68e-25 100
MsG0280009171.01.T01 MTR_0250s0040 34.659 176 33 5 22 192 1 99 1.81e-16 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACTAGTAGTAGGTGAATT+TGG 0.205689 2:+46826903 None:intergenic
CAGCAGCTGGTTATCTGATT+TGG 0.298434 2:-46828626 MsG0280009171.01.T01:intron
CGATATTGATAAGACAATTA+TGG 0.305371 2:-46827024 MsG0280009171.01.T01:CDS
TTCAGTGTCTCCAACTGAAT+TGG 0.327767 2:-46826826 MsG0280009171.01.T01:CDS
CACCAGTATCAAGGAAATTC+AGG 0.328339 2:+46827183 None:intergenic
CTGCAAGAGCAATGAAGCTT+TGG 0.357341 2:+46826698 None:intergenic
AAACAACAATGTTTAACTTC+GGG 0.367096 2:-46827110 MsG0280009171.01.T01:CDS
AAGTGTTACTGTTGAGACTA+TGG 0.367734 2:-46828686 MsG0280009171.01.T01:exon
AACCTGAATTTCCTTGATAC+TGG 0.413104 2:-46827185 MsG0280009171.01.T01:CDS
AAAAGGTCTCGTGCTAATAA+TGG 0.413380 2:-46826768 MsG0280009171.01.T01:CDS
GGTCAAATCCAGAAAATTGT+TGG 0.450466 2:+46826880 None:intergenic
TAAACAACAATGTTTAACTT+CGG 0.458515 2:-46827111 MsG0280009171.01.T01:CDS
ATTTAGTGATGAAACAAAGT+TGG 0.458588 2:-46827060 MsG0280009171.01.T01:CDS
TGCAAGAGCAATGAAGCTTT+GGG 0.460124 2:+46826699 None:intergenic
GTCAAATCCAGAAAATTGTT+GGG 0.480993 2:+46826881 None:intergenic
TTTGGAGGGCTGTCAAAAGA+TGG 0.482718 2:+46826921 None:intergenic
AATTGTTGGGAACTAGTAGT+AGG 0.495232 2:+46826894 None:intergenic
AATCTTCCACACCAGTATCA+AGG 0.520277 2:+46827174 None:intergenic
GGAACAGAATTTATTTGCTG+AGG 0.534779 2:-46827213 MsG0280009171.01.T01:CDS
TTGGAGGGCTGTCAAAAGAT+GGG 0.546086 2:+46826922 None:intergenic
CTAGTAGTAGGTGAATTTGG+AGG 0.546361 2:+46826906 None:intergenic
ACGTATCATCAAGCAGCAGC+TGG 0.560203 2:-46828639 MsG0280009171.01.T01:CDS
TAATGGTGAGGATCATATCA+TGG 0.568755 2:-46826751 MsG0280009171.01.T01:CDS
TAGTAGTAGGTGAATTTGGA+GGG 0.573251 2:+46826907 None:intergenic
AACAACAATGTTTAACTTCG+GGG 0.586447 2:-46827109 MsG0280009171.01.T01:CDS
GTCTCGTGCTAATAATGGTG+AGG 0.616632 2:-46826763 MsG0280009171.01.T01:CDS
GAATTTCCTTGATACTGGTG+TGG 0.624759 2:-46827180 MsG0280009171.01.T01:CDS
GTTGCTGAGAGTTGTGCAAG+TGG 0.638379 2:+46827085 None:intergenic
TATATACTGAATTTAAAACA+AGG 0.682236 2:+46828708 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAACTACAAAAAA+TGG - Chr2:46827555-46827574 MsG0280009171.01.T01:intron 10.0%
!! TAAAATAATAAAAAATGTGA+AGG - Chr2:46827198-46827217 MsG0280009171.01.T01:CDS 10.0%
!! TATCATTCTTTATAAAAATA+AGG - Chr2:46828055-46828074 MsG0280009171.01.T01:intron 10.0%
!! CAACATACAAATTATATTAT+GGG + Chr2:46827889-46827908 None:intergenic 15.0%
!!! AACATATATAAACATTCTTT+TGG + Chr2:46827423-46827442 None:intergenic 15.0%
!!! TATATACTGAATTTAAAACA+AGG + Chr2:46826717-46826736 None:intergenic 15.0%
!! ATATTTAAGAAACATTGCTA+GGG + Chr2:46827787-46827806 None:intergenic 20.0%
!! GCAACATACAAATTATATTA+TGG + Chr2:46827890-46827909 None:intergenic 20.0%
!! GCTAAAAAAAATTGATTATG+TGG - Chr2:46827715-46827734 MsG0280009171.01.T01:intron 20.0%
!! GTTATAAACATACTACAAAA+TGG + Chr2:46826919-46826938 None:intergenic 20.0%
!! TATATTTAAGAAACATTGCT+AGG + Chr2:46827788-46827807 None:intergenic 20.0%
!!! ATAGTTAGACTTTTTTGTAA+TGG + Chr2:46827298-46827317 None:intergenic 20.0%
!!! ATTGTTGTTTATTTTGTTCT+TGG + Chr2:46828302-46828321 None:intergenic 20.0%
!!! TAAACAACAATGTTTAACTT+CGG - Chr2:46828311-46828330 MsG0280009171.01.T01:intron 20.0%
!!! TGTAGTTACTTATTTTCAAA+AGG - Chr2:46828091-46828110 MsG0280009171.01.T01:intron 20.0%
!!! TTATATTATGGGTTTTTCTA+TGG + Chr2:46827878-46827897 None:intergenic 20.0%
!!! TTCATTTTGTTTTGAGTTTA+AGG + Chr2:46828571-46828590 None:intergenic 20.0%
! AACAGGTCAACAAATTAAAA+AGG + Chr2:46827268-46827287 None:intergenic 25.0%
! AATTTGTCCATGACAATATT+TGG + Chr2:46827373-46827392 None:intergenic 25.0%
! ACTTAGGGATATTATGTATT+AGG + Chr2:46826991-46827010 None:intergenic 25.0%
! ATTTAGTGATGAAACAAAGT+TGG - Chr2:46828362-46828381 MsG0280009171.01.T01:intron 25.0%
! ATTTGTCCATGACAATATTT+GGG + Chr2:46827372-46827391 None:intergenic 25.0%
! CAACCATTTATTTGAGAATT+TGG + Chr2:46828139-46828158 None:intergenic 25.0%
! CGATATTGATAAGACAATTA+TGG - Chr2:46828398-46828417 MsG0280009171.01.T01:intron 25.0%
! GATTAATATGTCATACGAAA+TGG - Chr2:46827606-46827625 MsG0280009171.01.T01:intron 25.0%
! TATTTGAGAATTTGGAGAAT+AGG + Chr2:46828131-46828150 None:intergenic 25.0%
! TCTCCAAATTCTCAAATAAA+TGG - Chr2:46828133-46828152 MsG0280009171.01.T01:intron 25.0%
! TTATTATTGATGTGTGTTAG+AGG - Chr2:46827742-46827761 MsG0280009171.01.T01:intron 25.0%
!! AAACAACAATGTTTAACTTC+GGG - Chr2:46828312-46828331 MsG0280009171.01.T01:intron 25.0%
!! TGAAGAAAGTTTTTGTGTAA+AGG + Chr2:46828463-46828482 None:intergenic 25.0%
!!! ACGGTAAACAATTCATTTTT+AGG - Chr2:46827170-46827189 MsG0280009171.01.T01:CDS 25.0%
!!! CTTAACTTGAAATATTTTGC+AGG + Chr2:46826872-46826891 None:intergenic 25.0%
!!! GTTTTCTTCTGTACTTTTTA+CGG - Chr2:46827151-46827170 MsG0280009171.01.T01:CDS 25.0%
!!! TATCTGATTTGGTAAGAAAA+TGG - Chr2:46826807-46826826 MsG0280009171.01.T01:CDS 25.0%
!!! TTAAAATTTCCTTTTTTCCC+AGG - Chr2:46828179-46828198 MsG0280009171.01.T01:intron 25.0%
!!! TTCTTATTTTCCAATTCAGT+TGG + Chr2:46828609-46828628 None:intergenic 25.0%
!!! TTTGTTTTGAGTTTAAGGTA+GGG + Chr2:46828566-46828585 None:intergenic 25.0%
!!! TTTTGTTTTGAGTTTAAGGT+AGG + Chr2:46828567-46828586 None:intergenic 25.0%
AAACTACAAAAAATGGAACC+AGG - Chr2:46827562-46827581 MsG0280009171.01.T01:intron 30.0%
AAATATGAGATGGTCAACTT+AGG + Chr2:46827007-46827026 None:intergenic 30.0%
AACTACAAAAAATGGAACCA+GGG - Chr2:46827563-46827582 MsG0280009171.01.T01:intron 30.0%
AACTTAGGACATAAGAGTTA+TGG + Chr2:46827928-46827947 None:intergenic 30.0%
AAGTTGACCATCTCATATTT+AGG - Chr2:46827007-46827026 MsG0280009171.01.T01:CDS 30.0%
AATATGAGATGGTCAACTTA+GGG + Chr2:46827006-46827025 None:intergenic 30.0%
ATGAGAACCTAAATATGAGA+TGG + Chr2:46827017-46827036 None:intergenic 30.0%
CAAAAACCTCAAAAAGCAAA+AGG - Chr2:46828637-46828656 MsG0280009171.01.T01:CDS 30.0%
CAATTAAGAGCAAGTTTGAT+TGG + Chr2:46827974-46827993 None:intergenic 30.0%
CAATTACTAAGAATATCGAC+AGG - Chr2:46827072-46827091 MsG0280009171.01.T01:CDS 30.0%
GATGACATGTTAGTTATACT+TGG + Chr2:46827243-46827262 None:intergenic 30.0%
GGTTCTCATGTACAATATTT+AGG - Chr2:46827028-46827047 MsG0280009171.01.T01:CDS 30.0%
GTCAAATCCAGAAAATTGTT+GGG + Chr2:46828544-46828563 None:intergenic 30.0%
GTTATGCTTCAGTATATGAA+CGG - Chr2:46827483-46827502 MsG0280009171.01.T01:intron 30.0%
TAGATTGAGAAATGCAACTT+AGG + Chr2:46827943-46827962 None:intergenic 30.0%
TTTGTCCATGACAATATTTG+GGG + Chr2:46827371-46827390 None:intergenic 30.0%
! AACAACAATGTTTAACTTCG+GGG - Chr2:46828313-46828332 MsG0280009171.01.T01:intron 30.0%
! TTTTTCCCCAAATATTGTCA+TGG - Chr2:46827363-46827382 MsG0280009171.01.T01:intron 30.0%
!! TTAATTTGTTGACCTGTTAC+CGG - Chr2:46827270-46827289 MsG0280009171.01.T01:intron 30.0%
!!! AGACTTTTTTGTAATGGATC+CGG + Chr2:46827292-46827311 None:intergenic 30.0%
!!! ATCTATACCGTTTTAGAGAT+AGG + Chr2:46827636-46827655 None:intergenic 30.0%
!!! TCTATACCGTTTTAGAGATA+GGG + Chr2:46827635-46827654 None:intergenic 30.0%
AAAAGGTCTCGTGCTAATAA+TGG - Chr2:46828654-46828673 MsG0280009171.01.T01:CDS 35.0%
AACCTGAATTTCCTTGATAC+TGG - Chr2:46828237-46828256 MsG0280009171.01.T01:intron 35.0%
AATGGAACCAGGGAAAATTA+AGG - Chr2:46827573-46827592 MsG0280009171.01.T01:intron 35.0%
AATTGTTGGGAACTAGTAGT+AGG + Chr2:46828531-46828550 None:intergenic 35.0%
GAACTAGTAGTAGGTGAATT+TGG + Chr2:46828522-46828541 None:intergenic 35.0%
GAGAATTTGGAGAATAGGAA+GGG + Chr2:46828126-46828145 None:intergenic 35.0%
GGGATATTATGTATTAGGAG+AGG + Chr2:46826986-46827005 None:intergenic 35.0%
GGTCAAATCCAGAAAATTGT+TGG + Chr2:46828545-46828564 None:intergenic 35.0%
TAATGGTGAGGATCATATCA+TGG - Chr2:46828671-46828690 MsG0280009171.01.T01:exon 35.0%
TAGTAGTAGGTGAATTTGGA+GGG + Chr2:46828518-46828537 None:intergenic 35.0%
TGAGAATTTGGAGAATAGGA+AGG + Chr2:46828127-46828146 None:intergenic 35.0%
TTCTAGATGAGACAGATTGA+TGG - Chr2:46827690-46827709 MsG0280009171.01.T01:intron 35.0%
! AAGTGTTACTGTTGAGACTA+TGG - Chr2:46826736-46826755 MsG0280009171.01.T01:CDS 35.0%
! ACTAGTTCCCAACAATTTTC+TGG - Chr2:46828534-46828553 MsG0280009171.01.T01:intron 35.0%
! GCAGGAAATTTCAAGTGTTA+AGG + Chr2:46826854-46826873 None:intergenic 35.0%
! GGAACAGAATTTATTTGCTG+AGG - Chr2:46828209-46828228 MsG0280009171.01.T01:intron 35.0%
! TGGAATCCCTATCTCTAAAA+CGG - Chr2:46827626-46827645 MsG0280009171.01.T01:intron 35.0%
!!! TTTCCTTTTTTCCCAGGAAA+TGG - Chr2:46828185-46828204 MsG0280009171.01.T01:intron 35.0%
AATCTTCCACACCAGTATCA+AGG + Chr2:46828251-46828270 None:intergenic 40.0%
AATTCTGTTCCTCCATTTCC+TGG + Chr2:46828200-46828219 None:intergenic 40.0%
ATTCTGTTCCTCCATTTCCT+GGG + Chr2:46828199-46828218 None:intergenic 40.0%
CACCAGTATCAAGGAAATTC+AGG + Chr2:46828242-46828261 None:intergenic 40.0%
CTAGTAGTAGGTGAATTTGG+AGG + Chr2:46828519-46828538 None:intergenic 40.0%
GAACCAGGGAAAATTAAGGA+TGG - Chr2:46827577-46827596 MsG0280009171.01.T01:intron 40.0%
GAATTTCCTTGATACTGGTG+TGG - Chr2:46828242-46828261 MsG0280009171.01.T01:intron 40.0%
TAGATGAGACAGATTGATGG+TGG - Chr2:46827693-46827712 MsG0280009171.01.T01:intron 40.0%
TGCAAGAGCAATGAAGCTTT+GGG + Chr2:46828726-46828745 None:intergenic 40.0%
TTCAGTGTCTCCAACTGAAT+TGG - Chr2:46828596-46828615 MsG0280009171.01.T01:intron 40.0%
! GTTCCATCCTTAATTTTCCC+TGG + Chr2:46827583-46827602 None:intergenic 40.0%
!! TTTGTAATGGATCCGGTAAC+AGG + Chr2:46827285-46827304 None:intergenic 40.0%
!!! ACGAGACCTTTTGCTTTTTG+AGG + Chr2:46828646-46828665 None:intergenic 40.0%
CAGCAGCTGGTTATCTGATT+TGG - Chr2:46826796-46826815 MsG0280009171.01.T01:CDS 45.0%
CCTCCATTTCCTGGGAAAAA+AGG + Chr2:46828191-46828210 None:intergenic 45.0%
CTGCAAGAGCAATGAAGCTT+TGG + Chr2:46828727-46828746 None:intergenic 45.0%
GTCTCGTGCTAATAATGGTG+AGG - Chr2:46828659-46828678 MsG0280009171.01.T01:CDS 45.0%
TCTGTCTCATCTAGAAGCTC+TGG + Chr2:46827686-46827705 None:intergenic 45.0%
TTGGAGGGCTGTCAAAAGAT+GGG + Chr2:46828503-46828522 None:intergenic 45.0%
TTTGGAGGGCTGTCAAAAGA+TGG + Chr2:46828504-46828523 None:intergenic 45.0%
!! AGGAGAGGCATTTGTATGTG+AGG + Chr2:46826971-46826990 None:intergenic 45.0%
!!! CCTTTTTTCCCAGGAAATGG+AGG - Chr2:46828188-46828207 MsG0280009171.01.T01:intron 45.0%
!!! GTATTTATTTAATTTAAAAA+TGG + Chr2:46827534-46827553 None:intergenic 5.0%
ACGTATCATCAAGCAGCAGC+TGG - Chr2:46826783-46826802 MsG0280009171.01.T01:CDS 50.0%
ACTCGTGACCAAGCCATTGA+TGG - Chr2:46827657-46827676 MsG0280009171.01.T01:intron 50.0%
GTTGCTGAGAGTTGTGCAAG+TGG + Chr2:46828340-46828359 None:intergenic 50.0%
TCTGGTGTCCATCAATGGCT+TGG + Chr2:46827668-46827687 None:intergenic 50.0%
! GAAGCTCTGGTGTCCATCAA+TGG + Chr2:46827673-46827692 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 46826692 46828752 46826692 ID=MsG0280009171.01;Name=MsG0280009171.01
Chr2 mRNA 46826692 46828752 46826692 ID=MsG0280009171.01.T01;Parent=MsG0280009171.01;Name=MsG0280009171.01.T01;_AED=0.30;_eAED=0.30;_QI=63|0.66|0.75|1|0|0.25|4|0|203
Chr2 exon 46828627 46828752 46828627 ID=MsG0280009171.01.T01:exon:5048;Parent=MsG0280009171.01.T01
Chr2 exon 46826692 46827240 46826692 ID=MsG0280009171.01.T01:exon:5049;Parent=MsG0280009171.01.T01
Chr2 five_prime_UTR 46828690 46828752 46828690 ID=MsG0280009171.01.T01:five_prime_utr;Parent=MsG0280009171.01.T01
Chr2 CDS 46828627 46828689 46828627 ID=MsG0280009171.01.T01:cds;Parent=MsG0280009171.01.T01
Chr2 CDS 46826692 46827240 46826692 ID=MsG0280009171.01.T01:cds;Parent=MsG0280009171.01.T01
Gene Sequence

>MsG0280009171.01.T01

ATGGAAGAAATCAACAACACAGCTATGAACGTATCATCAAGCAGCAGCTGGTTATCTGATTTGATGGAGGAACAGAATTTATTTGCTGAGGAATGCAACCTGAATTTCCTTGATACTGGTGTGGAAGATTTTCTTTCACATGACATAGCTAATGTCTTCCAAGAACAAAATAAACAACAATGTTTAACTTCGGGGTCCACTTGCACAACTCTCAGCAACACATTTAGTGATGAAACAAAGTTGGATTGTTTTGACTTCGATATTGATAAGACAATTATGGAGATGAAAACTATTGATCATAGTGACAAAATCAATGAAACCTTTACACAAAAACTTTCTTCATCTTTTCAAGTTCAAATCCCATCTTTTGACAGCCCTCCAAATTCACCTACTACTAGTTCCCAACAATTTTCTGGATTTGACCCTACCTTAAACTCAAAACAAAATGAAACAGTTTCAGTGTCTCCAACTGAATTGGAAAATAAGAATCATTCAACAAAAACCTCAAAAAGCAAAAGGTCTCGTGCTAATAATGGTGAGGATCATATCATGGCAGAGAGAGAGAAAGCGAAGAGAAACTCACCCAAAGCTTCATTGCTCTTGCAGCTCTAG

Protein sequence

>MsG0280009171.01.T01

MEEINNTAMNVSSSSSWLSDLMEEQNLFAEECNLNFLDTGVEDFLSHDIANVFQEQNKQQCLTSGSTCTTLSNTFSDETKLDCFDFDIDKTIMEMKTIDHSDKINETFTQKLSSSFQVQIPSFDSPPNSPTTSSQQFSGFDPTLNSKQNETVSVSPTELENKNHSTKTSKSKRSRANNGEDHIMAEREKAKRNSPKASLLLQL*