AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028285.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028285.01.T01 MTR_2g013570 100.000 53 0 0 4 56 47 99 2.44e-32 109
MsG0580028285.01.T01 MTR_2g013570 100.000 53 0 0 4 56 47 99 2.35e-31 109
MsG0580028285.01.T01 MTR_2g013570 100.000 53 0 0 4 56 47 99 5.76e-31 109
MsG0580028285.01.T01 MTR_6g007350 96.078 51 2 0 6 56 6 56 3.10e-29 100
MsG0580028285.01.T01 MTR_7g446330 96.078 51 2 0 6 56 22 72 4.14e-29 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 3 sgRNAs with CRISPR-Local

Find 8 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATGCAGAAAGTAGGATTC+AGG 0.399022 5:+77828191 MsG0580028285.01.T01:CDS
GCACTTGGAATGCAGAAAGT+AGG 0.491209 5:+77828184 MsG0580028285.01.T01:CDS
TAAGGCATGGTCTTAGCACT+TGG 0.512686 5:+77828169 MsG0580028285.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAGAAAAATCAATACTATT+TGG - Chr5:77828066-77828085 None:intergenic 20.0%
!!! AGTATTGATTTTTCTGTAAC+TGG + Chr5:77828069-77828088 MsG0580028285.01.T01:CDS 25.0%
! CAAAGAAAGTCACTCTTTTA+AGG + Chr5:77828151-77828170 MsG0580028285.01.T01:CDS 30.0%
!! ATTGATTTTTCTGTAACTGG+TGG + Chr5:77828072-77828091 MsG0580028285.01.T01:CDS 30.0%
!!! AAAGTCACTCTTTTAAGGCA+TGG + Chr5:77828156-77828175 MsG0580028285.01.T01:CDS 35.0%
GAATGCAGAAAGTAGGATTC+AGG + Chr5:77828191-77828210 MsG0580028285.01.T01:CDS 40.0%
GCACTTGGAATGCAGAAAGT+AGG + Chr5:77828184-77828203 MsG0580028285.01.T01:CDS 45.0%
! TAAGGCATGGTCTTAGCACT+TGG + Chr5:77828169-77828188 MsG0580028285.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr5 gene 77828045 77828242 77828045 ID=MsG0580028285.01;Name=MsG0580028285.01
Chr5 mRNA 77828045 77828242 77828045 ID=MsG0580028285.01.T01;Parent=MsG0580028285.01;Name=MsG0580028285.01.T01;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|0|1|0|65
Chr5 exon 77828045 77828242 77828045 ID=MsG0580028285.01.T01:exon:11640;Parent=MsG0580028285.01.T01
Chr5 CDS 77828045 77828242 77828045 ID=MsG0580028285.01.T01:cds;Parent=MsG0580028285.01.T01
Gene Sequence

>MsG0580028285.01.T01

CTTCATGCAGACAAACTTCCAAATAGTATTGATTTTTCTGTAACTGGTGGTGCATATGATTTCACAAAAGCAACAACATCACTAACAAATGAGTTAATCACTTCATCAAAGAAAGTCACTCTTTTAAGGCATGGTCTTAGCACTTGGAATGCAGAAAGTAGGATTCAGGTGATTTGCTCTCTACTTTTTAGTTATTAG

Protein sequence

>MsG0580028285.01.T01

LHADKLPNSIDFSVTGGAYDFTKATTSLTNELITSSKKVTLLRHGLSTWNAESRIQVICSLLFSY*