AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005969.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005969.01.T01 MTR_1g110930 98.122 213 3 1 8 219 148 360 6.91e-149 425
MsG0180005969.01.T01 MTR_5g056510 91.549 213 17 1 8 219 148 360 2.69e-138 398
MsG0180005969.01.T01 MTR_4g070270 69.091 220 67 1 9 227 161 380 6.84e-109 323
MsG0180005969.01.T01 MTR_1g076430 67.873 221 70 1 9 228 164 384 5.24e-107 319
MsG0180005969.01.T01 MTR_2g054940 72.170 212 58 1 9 219 163 374 9.28e-107 318
MsG0180005969.01.T01 MTR_1g041085 70.283 212 62 1 9 219 170 381 2.77e-105 315
MsG0180005969.01.T01 MTR_7g118170 69.194 211 64 1 10 219 184 394 9.31e-105 314
MsG0180005969.01.T01 MTR_3g105550 63.285 207 75 1 14 219 190 396 3.27e-96 292
MsG0180005969.01.T01 MTR_7g081695 68.868 212 53 2 9 219 163 362 6.08e-95 295
MsG0180005969.01.T01 MTR_4g113280 62.736 212 67 2 9 219 152 352 2.96e-90 275
MsG0180005969.01.T01 MTR_6g032965 56.364 220 94 2 9 227 42 260 6.99e-84 256
MsG0180005969.01.T01 MTR_7g118160 61.458 192 73 1 39 229 110 301 6.33e-82 252
MsG0180005969.01.T01 MTR_2g007020 54.717 212 95 1 9 219 109 320 2.87e-81 251
MsG0180005969.01.T01 MTR_4g054630 58.371 221 90 2 9 227 123 343 3.23e-81 251
MsG0180005969.01.T01 MTR_4g054415 58.371 221 90 2 9 227 82 302 3.29e-81 250
MsG0180005969.01.T01 MTR_6g074880 58.371 221 90 2 9 227 125 345 5.53e-81 251
MsG0180005969.01.T01 MTR_2g013740 55.505 218 96 1 14 230 178 395 4.14e-80 249
MsG0180005969.01.T01 MTR_2g436480 52.830 212 99 1 9 219 108 319 1.39e-76 239
MsG0180005969.01.T01 MTR_3g072980 55.140 214 93 3 8 219 143 355 1.08e-73 233
MsG0180005969.01.T01 MTR_3g072940 55.140 214 93 3 8 219 143 355 1.09e-73 233
MsG0180005969.01.T01 MTR_1g102290 51.643 213 102 1 8 219 142 354 6.28e-73 231
MsG0180005969.01.T01 MTR_6g051440 53.521 213 98 1 8 219 142 354 9.24e-66 212
MsG0180005969.01.T01 MTR_1g115560 43.534 232 130 1 13 243 98 329 1.59e-62 204
MsG0180005969.01.T01 MTR_2g096210 44.444 216 119 1 13 227 97 312 2.72e-58 192
MsG0180005969.01.T01 MTR_4g062270 39.640 222 133 1 13 233 97 318 2.16e-54 182
MsG0180005969.01.T01 MTR_4g062190 40.865 208 122 1 13 219 97 304 7.11e-54 181
MsG0180005969.01.T01 MTR_4g062170 40.196 204 121 1 13 215 97 300 4.66e-52 176
MsG0180005969.01.T01 MTR_4g054260 55.639 133 57 2 97 227 87 219 9.96e-41 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005969.01.T01 AT1G68530 83.568 213 34 1 8 219 149 361 4.10e-129 375
MsG0180005969.01.T01 AT1G25450 81.860 215 38 1 8 221 144 358 1.42e-127 371
MsG0180005969.01.T01 AT1G68530 82.587 201 34 1 8 207 149 349 1.42e-122 354
MsG0180005969.01.T01 AT2G16280 69.340 212 64 1 9 219 169 380 2.03e-107 320
MsG0180005969.01.T01 AT1G19440 62.295 244 89 2 8 250 172 413 4.42e-107 319
MsG0180005969.01.T01 AT4G34510 66.981 212 69 1 9 219 143 354 6.27e-104 310
MsG0180005969.01.T01 AT1G04220 68.692 214 64 2 9 219 166 379 1.29e-103 311
MsG0180005969.01.T01 AT1G01120 67.299 211 68 1 10 219 184 394 7.44e-102 306
MsG0180005969.01.T01 AT5G43760 69.907 216 60 2 9 219 172 387 1.74e-100 303
MsG0180005969.01.T01 AT2G26640 66.038 212 71 1 9 219 161 372 3.55e-96 291
MsG0180005969.01.T01 AT2G26250 62.319 207 77 1 14 219 187 393 5.70e-93 284
MsG0180005969.01.T01 AT2G46720 58.768 211 86 1 10 219 122 332 7.75e-92 279
MsG0180005969.01.T01 AT4G34520 57.466 221 93 1 8 227 147 367 1.39e-90 276
MsG0180005969.01.T01 AT2G15090 57.820 211 88 1 10 219 139 349 4.76e-84 259
MsG0180005969.01.T01 AT3G10280 55.924 211 85 2 10 219 122 325 4.81e-84 258
MsG0180005969.01.T01 AT4G34250 58.491 212 87 1 9 219 146 357 2.60e-83 258
MsG0180005969.01.T01 AT1G71160 50.943 212 103 1 9 219 108 319 7.22e-77 240
MsG0180005969.01.T01 AT5G49070 51.316 228 109 2 9 234 112 339 2.52e-71 226
MsG0180005969.01.T01 AT3G52160 49.138 232 102 4 7 222 27 258 3.81e-70 219
MsG0180005969.01.T01 AT5G49070 53.465 202 93 1 9 209 112 313 6.30e-70 218
MsG0180005969.01.T01 AT3G52160 49.138 232 102 4 7 222 140 371 1.87e-68 218
MsG0180005969.01.T01 AT5G04530 46.919 211 109 2 13 220 99 309 1.31e-61 201
MsG0180005969.01.T01 AT2G28630 43.062 209 118 1 12 219 96 304 1.48e-59 196
MsG0180005969.01.T01 AT2G28630 43.062 209 118 1 12 219 96 304 1.48e-59 196
MsG0180005969.01.T01 AT1G07720 41.951 205 118 1 12 215 96 300 2.08e-55 185
MsG0180005969.01.T01 AT1G07720 41.951 205 118 1 12 215 139 343 2.58e-55 186

Find 38 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAAAGTTTCAATCTTTC+TGG 0.065209 1:-98272614 MsG0180005969.01.T01:CDS
GGAATATATGGTTTCCATTT+AGG 0.109972 1:+98272144 None:intergenic
CTAACAACAGCGTTTGAATT+TGG 0.224482 1:+98272530 None:intergenic
CCCATTCTGAACAAACAATT+TGG 0.235185 1:+98272452 None:intergenic
ATGAGTATGTCTATGTCTTT+TGG 0.254903 1:+98272713 None:intergenic
GAATATATGGTTTCCATTTA+GGG 0.289900 1:+98272145 None:intergenic
GCTGATTCCATGGTTGGTTT+TGG 0.301961 1:+98272803 None:intergenic
TGTTTAAAATCAGGAATATA+TGG 0.307224 1:+98272132 None:intergenic
ACCAAATTGTTTGTTCAGAA+TGG 0.334078 1:-98272453 MsG0180005969.01.T01:CDS
CCTCTTGCTGATTCCATGGT+TGG 0.334706 1:+98272797 None:intergenic
GAAGACAAAGAAGGTAAAGT+TGG 0.337284 1:-98272305 MsG0180005969.01.T01:CDS
TCATTTCCTTGGTAATAGTT+TGG 0.338601 1:+98272491 None:intergenic
TCAAAAGCTTGTTTAAAATC+AGG 0.357770 1:+98272123 None:intergenic
GCATGGCTCTTTCATTTCCT+TGG 0.383297 1:+98272480 None:intergenic
AGTTTCAATCTTTCTGGAAT+GGG 0.384158 1:-98272608 MsG0180005969.01.T01:CDS
AAAGATCTTATGGCAATTGC+AGG 0.402261 1:-98272269 MsG0180005969.01.T01:CDS
CCAAATTGTTTGTTCAGAAT+GGG 0.412558 1:-98272452 MsG0180005969.01.T01:CDS
TTCACCTCTTGCTGATTCCA+TGG 0.414626 1:+98272793 None:intergenic
TCTGGAATGGGGTGTAGTGC+TGG 0.419176 1:-98272596 MsG0180005969.01.T01:CDS
AACACCTTCTTTGTCTGCTA+TGG 0.422563 1:-98272666 MsG0180005969.01.T01:CDS
AAGTTTCAATCTTTCTGGAA+TGG 0.430041 1:-98272609 MsG0180005969.01.T01:CDS
TTTGAAGAAGAAGACAAAGA+AGG 0.458785 1:-98272314 MsG0180005969.01.T01:CDS
AAATCAAATATAACAACAAT+GGG 0.469834 1:-98272236 MsG0180005969.01.T01:CDS
TGGTGGAATATATGAATTGA+AGG 0.472720 1:+98272823 None:intergenic
AAAAGTAACTGTTCTGAAGC+TGG 0.486547 1:+98272200 None:intergenic
CATGTTGTTAGAACACATAA+AGG 0.489283 1:-98272365 MsG0180005969.01.T01:CDS
TAAATCAAATATAACAACAA+TGG 0.543034 1:-98272237 MsG0180005969.01.T01:CDS
GTTTCAATCTTTCTGGAATG+GGG 0.553526 1:-98272607 MsG0180005969.01.T01:CDS
TATTCCACCAAAACCAACCA+TGG 0.572625 1:-98272810 MsG0180005969.01.T01:CDS
ATAGCAGACAAAGAAGGTGT+TGG 0.589581 1:+98272668 None:intergenic
CAAACAATTTGGTAAAAGCA+TGG 0.599779 1:+98272463 None:intergenic
ATAACACCAAACTATTACCA+AGG 0.604070 1:-98272497 MsG0180005969.01.T01:CDS
ATCACCATAGCAGACAAAGA+AGG 0.607711 1:+98272662 None:intergenic
TCTGAAGCTGGTAAAACAAG+AGG 0.631454 1:+98272212 None:intergenic
AATTGTTTGTTCAGAATGGG+TGG 0.644773 1:-98272449 MsG0180005969.01.T01:CDS
GTTGTTAGAACACATAAAGG+AGG 0.674114 1:-98272362 MsG0180005969.01.T01:CDS
TGGAATATATGAATTGAAGG+TGG 0.688122 1:+98272826 None:intergenic
CCAACCATGGAATCAGCAAG+AGG 0.689762 1:-98272797 MsG0180005969.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCAAATATAACAACAAT+GGG - Chr1:98272682-98272701 MsG0180005969.01.T01:CDS 15.0%
!! TAAATCAAATATAACAACAA+TGG - Chr1:98272681-98272700 MsG0180005969.01.T01:CDS 15.0%
!!! TGTTTAAAATCAGGAATATA+TGG + Chr1:98272789-98272808 None:intergenic 20.0%
! ATCAAAAGTTTCAATCTTTC+TGG - Chr1:98272304-98272323 MsG0180005969.01.T01:CDS 25.0%
! ATCACTTCAAAAAGATCTTA+TGG - Chr1:98272639-98272658 MsG0180005969.01.T01:CDS 25.0%
! GAATATATGGTTTCCATTTA+GGG + Chr1:98272776-98272795 None:intergenic 25.0%
!! GATTCTTTGTTTGAAAAAAC+CGG - Chr1:98272175-98272194 MsG0180005969.01.T01:CDS 25.0%
!! TCAAAAGCTTGTTTAAAATC+AGG + Chr1:98272798-98272817 None:intergenic 25.0%
!!! TTTTTCAAACAAAGAATCCA+TGG + Chr1:98272173-98272192 None:intergenic 25.0%
ACCAAATTGTTTGTTCAGAA+TGG - Chr1:98272465-98272484 MsG0180005969.01.T01:CDS 30.0%
ATAACACCAAACTATTACCA+AGG - Chr1:98272421-98272440 MsG0180005969.01.T01:CDS 30.0%
CAAACAATTTGGTAAAAGCA+TGG + Chr1:98272458-98272477 None:intergenic 30.0%
CATGTTGTTAGAACACATAA+AGG - Chr1:98272553-98272572 MsG0180005969.01.T01:CDS 30.0%
CCAAATTGTTTGTTCAGAAT+GGG - Chr1:98272466-98272485 MsG0180005969.01.T01:CDS 30.0%
GAAAAATCTTCAACCCTAAA+TGG - Chr1:98272760-98272779 MsG0180005969.01.T01:CDS 30.0%
GGAATATATGGTTTCCATTT+AGG + Chr1:98272777-98272796 None:intergenic 30.0%
TGGAATATATGAATTGAAGG+TGG + Chr1:98272095-98272114 None:intergenic 30.0%
TGGTGGAATATATGAATTGA+AGG + Chr1:98272098-98272117 None:intergenic 30.0%
TTTGAAGAAGAAGACAAAGA+AGG - Chr1:98272604-98272623 MsG0180005969.01.T01:CDS 30.0%
! AAGTTTCAATCTTTCTGGAA+TGG - Chr1:98272309-98272328 MsG0180005969.01.T01:CDS 30.0%
! ATGAGTATGTCTATGTCTTT+TGG + Chr1:98272208-98272227 None:intergenic 30.0%
! TCATTTCCTTGGTAATAGTT+TGG + Chr1:98272430-98272449 None:intergenic 30.0%
! TGACATTTTTGTATACATGC+TGG - Chr1:98272815-98272834 MsG0180005969.01.T01:CDS 30.0%
!! AGTTTCAATCTTTCTGGAAT+GGG - Chr1:98272310-98272329 MsG0180005969.01.T01:CDS 30.0%
!!! TATGTCTTTTGGTTTAAGAC+CGG + Chr1:98272197-98272216 None:intergenic 30.0%
AAAGATCTTATGGCAATTGC+AGG - Chr1:98272649-98272668 MsG0180005969.01.T01:CDS 35.0%
AATTGTTTGTTCAGAATGGG+TGG - Chr1:98272469-98272488 MsG0180005969.01.T01:CDS 35.0%
CCCATTCTGAACAAACAATT+TGG + Chr1:98272469-98272488 None:intergenic 35.0%
CTAACAACAGCGTTTGAATT+TGG + Chr1:98272391-98272410 None:intergenic 35.0%
GAAGACAAAGAAGGTAAAGT+TGG - Chr1:98272613-98272632 MsG0180005969.01.T01:CDS 35.0%
GTTGTTAGAACACATAAAGG+AGG - Chr1:98272556-98272575 MsG0180005969.01.T01:CDS 35.0%
! ACTTTTCTCATCACACTCAT+TGG - Chr1:98272735-98272754 MsG0180005969.01.T01:CDS 35.0%
! CATTTTTGTATACATGCTGG+TGG - Chr1:98272818-98272837 MsG0180005969.01.T01:CDS 35.0%
!! AAAAGTAACTGTTCTGAAGC+TGG + Chr1:98272721-98272740 None:intergenic 35.0%
!! GTTTCAATCTTTCTGGAATG+GGG - Chr1:98272311-98272330 MsG0180005969.01.T01:CDS 35.0%
!!! ACTTGTTATTTTCTCAGCCA+TGG - Chr1:98272153-98272172 MsG0180005969.01.T01:CDS 35.0%
AACACCTTCTTTGTCTGCTA+TGG - Chr1:98272252-98272271 MsG0180005969.01.T01:CDS 40.0%
ATCACCATAGCAGACAAAGA+AGG + Chr1:98272259-98272278 None:intergenic 40.0%
TATTCCACCAAAACCAACCA+TGG - Chr1:98272108-98272127 MsG0180005969.01.T01:CDS 40.0%
! ATAGCAGACAAAGAAGGTGT+TGG + Chr1:98272253-98272272 None:intergenic 40.0%
! TCTGAAGCTGGTAAAACAAG+AGG + Chr1:98272709-98272728 None:intergenic 40.0%
GCATGGCTCTTTCATTTCCT+TGG + Chr1:98272441-98272460 None:intergenic 45.0%
!! TTCACCTCTTGCTGATTCCA+TGG + Chr1:98272128-98272147 None:intergenic 45.0%
!!! GATTCCATGGTTGGTTTTGG+TGG + Chr1:98272115-98272134 None:intergenic 45.0%
!!! GCTGATTCCATGGTTGGTTT+TGG + Chr1:98272118-98272137 None:intergenic 45.0%
CCAACCATGGAATCAGCAAG+AGG - Chr1:98272121-98272140 MsG0180005969.01.T01:CDS 50.0%
!! CCTCTTGCTGATTCCATGGT+TGG + Chr1:98272124-98272143 None:intergenic 50.0%
! TCTGGAATGGGGTGTAGTGC+TGG - Chr1:98272322-98272341 MsG0180005969.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 98272085 98272855 98272085 ID=MsG0180005969.01;Name=MsG0180005969.01
Chr1 mRNA 98272085 98272855 98272085 ID=MsG0180005969.01.T01;Parent=MsG0180005969.01;Name=MsG0180005969.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|256
Chr1 exon 98272085 98272855 98272085 ID=MsG0180005969.01.T01:exon:19693;Parent=MsG0180005969.01.T01
Chr1 CDS 98272085 98272855 98272085 ID=MsG0180005969.01.T01:cds;Parent=MsG0180005969.01.T01
Gene Sequence

>MsG0180005969.01.T01

ATGTTTACCACCTTCAATTCATATATTCCACCAAAACCAACCATGGAATCAGCAAGAGGTGAAGCTGAACTTGTTATTTTCTCAGCCATGGATTCTTTGTTTGAAAAAACCGGTCTTAAACCAAAAGACATAGACATACTCATAGTAAATTGTAGCTTGTTTTCTCCAACACCTTCTTTGTCTGCTATGGTGATTAACAAATACAAATTAAGAAGCAATATCAAAAGTTTCAATCTTTCTGGAATGGGGTGTAGTGCTGGACTCATTTCGATCGATTTAGCTCGTGATCTTCTTCAAGTTCATCCAAATTCAAACGCTGTTGTTAGCACAGAGATTATAACACCAAACTATTACCAAGGAAATGAAAGAGCCATGCTTTTACCAAATTGTTTGTTCAGAATGGGTGGTGCTGCTATTCTGTTATCAAACAGAACATCAGAGAAAAGAAGAGCAAAATACAGATTAGTTCATGTTGTTAGAACACATAAAGGAGGTGATGATAAAGCATATAAATGTGTTTTTGAAGAAGAAGACAAAGAAGGTAAAGTTGGAATATCACTTCAAAAAGATCTTATGGCAATTGCAGGTGAAGCTCTTAAATCAAATATAACAACAATGGGTCCTCTTGTTTTACCAGCTTCAGAACAGTTACTTTTCTCATCACACTCATTGGTAGAAAAATCTTCAACCCTAAATGGAAACCATATATTCCTGATTTTAAACAAGCTTTTGACATTTTTGTATACATGCTGGTGGTCGTGCTGTGATTGA

Protein sequence

>MsG0180005969.01.T01

MFTTFNSYIPPKPTMESARGEAELVIFSAMDSLFEKTGLKPKDIDILIVNCSLFSPTPSLSAMVINKYKLRSNIKSFNLSGMGCSAGLISIDLARDLLQVHPNSNAVVSTEIITPNYYQGNERAMLLPNCLFRMGGAAILLSNRTSEKRRAKYRLVHVVRTHKGGDDKAYKCVFEEEDKEGKVGISLQKDLMAIAGEALKSNITTMGPLVLPASEQLLFSSHSLVEKSSTLNGNHIFLILNKLLTFLYTCWWSCCD*