Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013887.01.T01 | YP_001381741.1 | 99.167 | 120 | 1 | 0 | 1 | 120 | 1 | 120 | 2.21E-75 | 229 |
| MsG0380013887.01.T02 | CAF5212638.1 | 100 | 70 | 0 | 0 | 1 | 70 | 41 | 110 | 3.34E-40 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013887.01.T01 | B5LML1 | 96.667 | 120 | 4 | 0 | 1 | 120 | 1 | 120 | 6.08E-76 | 223 |
| MsG0380013887.01.T02 | P06258 | 97.143 | 70 | 2 | 0 | 1 | 70 | 51 | 120 | 4.15E-42 | 135 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013887.01.T01 | A0A4P8F3K1 | 99.167 | 120 | 1 | 0 | 1 | 120 | 1 | 120 | 1.05e-75 | 229 |
| MsG0380013887.01.T02 | A0A822IB16 | 100.000 | 70 | 0 | 0 | 1 | 70 | 41 | 110 | 1.60e-40 | 137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048059.01 | MsG0380013887.01 | 0.809236 | 2.091063e-50 | 1.221163e-47 |
| MsG0080048560.01 | MsG0380013887.01 | 0.818857 | 1.574765e-52 | 1.192566e-49 |
| MsG0080048689.01 | MsG0380013887.01 | 0.824238 | 8.994294e-54 | 7.921267e-51 |
| MsG0080048690.01 | MsG0380013887.01 | 0.809696 | 1.665265e-50 | 9.844799e-48 |
| MsG0080048691.01 | MsG0380013887.01 | 0.827774 | 1.299333e-54 | 1.266624e-51 |
| MsG0080048909.01 | MsG0380013887.01 | 0.811827 | 5.761277e-51 | 3.603064e-48 |
| MsG0080049077.01 | MsG0380013887.01 | 0.807453 | 5.017793e-50 | 2.795999e-47 |
| MsG0180000325.01 | MsG0380013887.01 | 0.804844 | 1.777597e-49 | 9.262434e-47 |
| MsG0180001919.01 | MsG0380013887.01 | 0.807958 | 3.919348e-50 | 2.212853e-47 |
| MsG0380012444.01 | MsG0380013887.01 | 0.811748 | 5.995598e-51 | 3.741742e-48 |
| MsG0380013448.01 | MsG0380013887.01 | 0.811821 | 5.778923e-51 | 3.613558e-48 |
| MsG0380013887.01 | MsG0380013888.01 | 0.832895 | 7.281380e-56 | 8.250138e-53 |
| MsG0380013887.01 | MsG0380013917.01 | 0.820744 | 5.834873e-53 | 4.656006e-50 |
| MsG0380013887.01 | MsG0380013919.01 | 0.828040 | 1.121297e-54 | 1.101732e-51 |
| MsG0380013887.01 | MsG0380013921.01 | 0.824698 | 7.011232e-54 | 6.257693e-51 |
| MsG0380013887.01 | MsG0380013926.01 | 0.823505 | 1.336290e-53 | 1.152788e-50 |
| MsG0380013887.01 | MsG0380013929.01 | 0.805322 | 1.411446e-49 | 7.445092e-47 |
| MsG0380013887.01 | MsG0380013931.01 | 0.822235 | 2.640097e-53 | 2.196995e-50 |
| MsG0380013887.01 | MsG0380013935.01 | 0.819056 | 1.419086e-52 | 1.080542e-49 |
| MsG0380013887.01 | MsG0380013938.01 | 0.806075 | 9.810790e-50 | 5.275875e-47 |
| MsG0380013887.01 | MsG0380013941.01 | 0.818217 | 2.199332e-52 | 1.636231e-49 |
| MsG0380013887.01 | MsG0380013952.01 | 0.816955 | 4.234786e-52 | 3.043765e-49 |
| MsG0380013887.01 | MsG0380013956.01 | 0.815957 | 7.083990e-52 | 4.953856e-49 |
| MsG0380013887.01 | MsG0380013964.01 | 0.813571 | 2.393155e-51 | 1.568457e-48 |
| MsG0380013887.01 | MsG0380013966.01 | 0.811687 | 6.180843e-51 | 3.851035e-48 |
| MsG0380013887.01 | MsG0380013969.01 | 0.814139 | 1.793472e-51 | 1.193699e-48 |
| MsG0380013887.01 | MsG0380013971.01 | 0.801863 | 7.367413e-49 | 3.556574e-46 |
| MsG0380013887.01 | MsG0380013972.01 | 0.801521 | 8.657035e-49 | 4.143013e-46 |
| MsG0380013887.01 | MsG0380013975.01 | 0.811358 | 7.286962e-51 | 4.500886e-48 |
| MsG0380013887.01 | MsG0380013978.01 | 0.825324 | 4.988878e-54 | 4.532763e-51 |
| MsG0380013887.01 | MsG0380013983.01 | 0.807460 | 5.000327e-50 | 2.786765e-47 |
| MsG0380013887.01 | MsG0380013996.01 | 0.836755 | 7.769230e-57 | 9.885134e-54 |
| MsG0380013887.01 | MsG0380013999.01 | 0.825296 | 5.063282e-54 | 4.596605e-51 |
| MsG0380013887.01 | MsG0380014004.01 | 0.824198 | 9.192920e-54 | 8.086957e-51 |
| MsG0380013887.01 | MsG0380014017.01 | 0.803826 | 2.895941e-49 | 1.469879e-46 |
| MsG0380013887.01 | MsG0380014021.01 | 0.813895 | 2.030822e-51 | 1.342750e-48 |
| MsG0380013887.01 | MsG0380014023.01 | 0.808078 | 3.696095e-50 | 2.093389e-47 |
| MsG0380013887.01 | MsG0380014024.01 | 0.811336 | 7.365497e-51 | 4.546832e-48 |
| MsG0380013887.01 | MsG0380014026.01 | 0.816799 | 4.591030e-52 | 3.285280e-49 |
| MsG0380013887.01 | MsG0380014028.01 | 0.808563 | 2.912320e-50 | 1.670622e-47 |
| MsG0380013887.01 | MsG0380014034.01 | 0.851913 | 6.511179e-61 | 1.337818e-57 |
| MsG0380013887.01 | MsG0380014050.01 | 0.833350 | 5.608766e-56 | 6.442728e-53 |
| MsG0380013887.01 | MsG0380015348.01 | 0.810752 | 9.860586e-51 | 5.993979e-48 |
| MsG0380013887.01 | MsG0380016531.01 | 0.804037 | 2.618187e-49 | 1.336134e-46 |
| MsG0380013887.01 | MsG0480022296.01 | 0.819110 | 1.379057e-52 | 1.051629e-49 |
| MsG0380013887.01 | MsG0480023006.01 | 0.802376 | 5.776190e-49 | 2.825190e-46 |
| MsG0380013887.01 | MsG0580027095.01 | 0.804408 | 2.191837e-49 | 1.129198e-46 |
| MsG0380013887.01 | MsG0580027677.01 | 0.805249 | 1.462158e-49 | 7.697865e-47 |
| MsG0380013887.01 | MsG0580029820.01 | 0.803827 | 2.895083e-49 | 1.469466e-46 |
| MsG0380013887.01 | MsG0780037988.01 | 0.804458 | 2.139035e-49 | 1.103462e-46 |
| MsG0380013887.01 | MsG0780038197.01 | 0.805076 | 1.589793e-49 | 8.332901e-47 |
| MsG0380013887.01 | MsG0780040321.01 | 0.800686 | 1.282969e-48 | 6.011385e-46 |
| MsG0380013887.01 | MsG0880044440.01 | 0.804770 | 1.841259e-49 | 9.575725e-47 |
| MsG0380013887.01 | MsG0880045188.01 | 0.809041 | 2.302085e-50 | 1.337337e-47 |
| MsG0380013887.01 | MsG0880046469.01 | 0.805123 | 1.554130e-49 | 8.155980e-47 |
| MsG0280010137.01 | MsG0380013887.01 | 0.834665 | 2.628043e-56 | 3.139757e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013887.01.T01 | MTR_4g051245 | 99.167 | 120 | 1 | 0 | 1 | 120 | 1 | 120 | 2.67e-79 | 229 |
| MsG0380013887.01.T01 | MTR_3g035740 | 98.571 | 70 | 1 | 0 | 1 | 70 | 36 | 105 | 4.02e-43 | 138 |
| MsG0380013887.01.T01 | MTR_5g090760 | 86.207 | 58 | 8 | 0 | 1 | 58 | 127 | 184 | 3.67e-27 | 99.4 |
| MsG0380013887.01.T01 | MTR_0082s0040 | 39.241 | 79 | 48 | 0 | 38 | 116 | 152 | 230 | 4.03e-15 | 70.1 |
| MsG0380013887.01.T01 | MTR_7g076390 | 76.744 | 43 | 10 | 0 | 1 | 43 | 7 | 49 | 5.90e-15 | 64.7 |
| MsG0380013887.01.T02 | MTR_4g051245 | 100.000 | 70 | 0 | 0 | 1 | 70 | 51 | 120 | 6.40e-44 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013887.01.T01 | ATCG00440 | 90.000 | 120 | 12 | 0 | 1 | 120 | 1 | 120 | 4.80e-73 | 213 |
| MsG0380013887.01.T01 | ATMG00990 | 39.506 | 81 | 49 | 0 | 38 | 118 | 36 | 116 | 3.44e-18 | 74.3 |
| MsG0380013887.01.T01 | AT2G07751 | 39.506 | 81 | 49 | 0 | 38 | 118 | 36 | 116 | 3.44e-18 | 74.3 |
| MsG0380013887.01.T02 | ATCG00440 | 94.286 | 70 | 4 | 0 | 1 | 70 | 51 | 120 | 1.06e-41 | 132 |
| MsG0380013887.01.T02 | ATMG00990 | 34.328 | 67 | 44 | 0 | 2 | 68 | 50 | 116 | 5.00e-11 | 54.3 |
| MsG0380013887.01.T02 | AT2G07751 | 34.328 | 67 | 44 | 0 | 2 | 68 | 50 | 116 | 5.00e-11 | 54.3 |
Find 22 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGATCTTTATTCCTATTT+TGG | 0.106455 | 3:+47518695 | MsG0380013887.01.T01:CDS |
| GAAATCAGAAATGCCAAAAT+AGG | 0.275054 | 3:-47518708 | None:intergenic |
| AAACTTTCTAGTTATGAATC+TGG | 0.326530 | 3:+47518765 | MsG0380013887.01.T01:CDS |
| TTCGTCCTTATCCTAATTGT+TGG | 0.355204 | 3:+47518945 | MsG0380013887.01.T01:CDS |
| CATAAACTAAACCAACAATT+AGG | 0.360505 | 3:-47518956 | None:intergenic |
| GATGAAAGCTTCTATAAATA+CGG | 0.441914 | 3:-47518922 | None:intergenic |
| AACTTTCTAGTTATGAATCT+GGG | 0.461336 | 3:+47518766 | MsG0380013887.01.T01:CDS |
| AAACAGTCTTTCTTTACCCA+TGG | 0.490928 | 3:+47518871 | MsG0380013887.01.T01:CDS |
| CTAAACCAACAATTAGGATA+AGG | 0.503195 | 3:-47518950 | None:intergenic |
| TAGAACCGATGGGCGATGCT+TGG | 0.504444 | 3:+47518790 | MsG0380013887.01.T01:CDS |
| TTGTTGGTTTAGTTTATGCA+TGG | 0.529511 | 3:+47518961 | MsG0380013887.01.T01:CDS |
| AACAGTCTTTCTTTACCCAT+GGG | 0.543993 | 3:+47518872 | MsG0380013887.01.T01:CDS |
| GGCGGAAGGGAGCATTAGAA+TGG | 0.551011 | 3:+47518982 | MsG0380013887.01.T01:CDS |
| TTTAGTTTATGCATGGCGGA+AGG | 0.557911 | 3:+47518968 | MsG0380013887.01.T01:CDS |
| TGAATCTGGGATAGAACCGA+TGG | 0.558151 | 3:+47518779 | MsG0380013887.01.T01:CDS |
| ACATCAAAACTCATTGCCCA+TGG | 0.560584 | 3:-47518888 | None:intergenic |
| CTAGAGCAAACATATAATAA+CGG | 0.568609 | 3:-47518827 | None:intergenic |
| TTAGTTTATGCATGGCGGAA+GGG | 0.588132 | 3:+47518969 | MsG0380013887.01.T01:CDS |
| CATCAAAACTCATTGCCCAT+GGG | 0.596446 | 3:-47518887 | None:intergenic |
| TGTAACCAAGCATCGCCCAT+CGG | 0.642657 | 3:-47518795 | None:intergenic |
| GAATCTGGGATAGAACCGAT+GGG | 0.671944 | 3:+47518780 | MsG0380013887.01.T01:CDS |
| TTGGTTTAGTTTATGCATGG+CGG | 0.690036 | 3:+47518964 | MsG0380013887.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATGAATATGATATTTTT+TGG | + | Chr3:47518658-47518677 | MsG0380013887.01.T01:CDS | 10.0% |
| !!! | ATTTTAGCTCTAATTAGAAA+AGG | + | Chr3:47518735-47518754 | MsG0380013887.01.T01:CDS | 20.0% |
| !!! | TTTTAGCTCTAATTAGAAAA+GGG | + | Chr3:47518736-47518755 | MsG0380013887.01.T01:CDS | 20.0% |
| ! | CATAAACTAAACCAACAATT+AGG | - | Chr3:47518959-47518978 | None:intergenic | 25.0% |
| ! | CTAGAGCAAACATATAATAA+CGG | - | Chr3:47518830-47518849 | None:intergenic | 25.0% |
| ! | GATGAAAGCTTCTATAAATA+CGG | - | Chr3:47518925-47518944 | None:intergenic | 25.0% |
| !! | ATCGATCTTTATTCCTATTT+TGG | + | Chr3:47518695-47518714 | MsG0380013887.01.T01:CDS | 25.0% |
| !! | TCATAACTAGAAAGTTTTTC+TGG | - | Chr3:47518762-47518781 | None:intergenic | 25.0% |
| !!! | AAACTTTCTAGTTATGAATC+TGG | + | Chr3:47518765-47518784 | MsG0380013887.01.T01:CDS | 25.0% |
| !!! | AACTTTCTAGTTATGAATCT+GGG | + | Chr3:47518766-47518785 | MsG0380013887.01.T01:CDS | 25.0% |
| CTAAACCAACAATTAGGATA+AGG | - | Chr3:47518953-47518972 | None:intergenic | 30.0% | |
| GAAATCAGAAATGCCAAAAT+AGG | - | Chr3:47518711-47518730 | None:intergenic | 30.0% | |
| !! | AATGAGTTTTGATGTATTGG+GGG | + | Chr3:47518896-47518915 | MsG0380013887.01.T01:CDS | 30.0% |
| !! | ATTTTGGCATTTCTGATTTC+TGG | + | Chr3:47518711-47518730 | MsG0380013887.01.T01:CDS | 30.0% |
| !! | CAATGAGTTTTGATGTATTG+GGG | + | Chr3:47518895-47518914 | MsG0380013887.01.T01:CDS | 30.0% |
| !! | GCAATGAGTTTTGATGTATT+GGG | + | Chr3:47518894-47518913 | MsG0380013887.01.T01:CDS | 30.0% |
| !! | TTGTTGGTTTAGTTTATGCA+TGG | + | Chr3:47518961-47518980 | MsG0380013887.01.T01:CDS | 30.0% |
| AAACAGTCTTTCTTTACCCA+TGG | + | Chr3:47518871-47518890 | MsG0380013887.01.T01:CDS | 35.0% | |
| AACAGTCTTTCTTTACCCAT+GGG | + | Chr3:47518872-47518891 | MsG0380013887.01.T01:CDS | 35.0% | |
| TTCGTCCTTATCCTAATTGT+TGG | + | Chr3:47518945-47518964 | MsG0380013887.01.T01:CDS | 35.0% | |
| ! | TTGGTTTAGTTTATGCATGG+CGG | + | Chr3:47518964-47518983 | MsG0380013887.01.T01:CDS | 35.0% |
| !! | GGCAATGAGTTTTGATGTAT+TGG | + | Chr3:47518893-47518912 | MsG0380013887.01.T01:CDS | 35.0% |
| ACATCAAAACTCATTGCCCA+TGG | - | Chr3:47518891-47518910 | None:intergenic | 40.0% | |
| CATCAAAACTCATTGCCCAT+GGG | - | Chr3:47518890-47518909 | None:intergenic | 40.0% | |
| ! | TTAGTTTATGCATGGCGGAA+GGG | + | Chr3:47518969-47518988 | MsG0380013887.01.T01:CDS | 40.0% |
| ! | TTTAGTTTATGCATGGCGGA+AGG | + | Chr3:47518968-47518987 | MsG0380013887.01.T01:CDS | 40.0% |
| ! | GAATCTGGGATAGAACCGAT+GGG | + | Chr3:47518780-47518799 | MsG0380013887.01.T01:CDS | 45.0% |
| ! | TGAATCTGGGATAGAACCGA+TGG | + | Chr3:47518779-47518798 | MsG0380013887.01.T01:CDS | 45.0% |
| ! | TGTAACCAAGCATCGCCCAT+CGG | - | Chr3:47518798-47518817 | None:intergenic | 50.0% |
| TAGAACCGATGGGCGATGCT+TGG | + | Chr3:47518790-47518809 | MsG0380013887.01.T01:CDS | 55.0% | |
| !! | GGCGGAAGGGAGCATTAGAA+TGG | + | Chr3:47518982-47519001 | MsG0380013887.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 47518648 | 47519010 | 47518648 | ID=MsG0380013887.01;Name=MsG0380013887.01 |
| Chr3 | mRNA | 47518648 | 47519010 | 47518648 | ID=MsG0380013887.01.T01;Parent=MsG0380013887.01;Name=MsG0380013887.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|120 |
| Chr3 | exon | 47518648 | 47519010 | 47518648 | ID=MsG0380013887.01.T01:exon:26398;Parent=MsG0380013887.01.T01 |
| Chr3 | CDS | 47518648 | 47519010 | 47518648 | ID=MsG0380013887.01.T01:cds;Parent=MsG0380013887.01.T01 |
| Chr3 | mRNA | 47518798 | 47519010 | 47518798 | ID=MsG0380013887.01.T02;Parent=MsG0380013887.01;Name=MsG0380013887.01.T02;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|70 |
| Chr3 | exon | 47518798 | 47519010 | 47518798 | ID=MsG0380013887.01.T02:exon:26399;Parent=MsG0380013887.01.T02 |
| Chr3 | CDS | 47518798 | 47519010 | 47518798 | ID=MsG0380013887.01.T02:cds;Parent=MsG0380013887.01.T02 |
| Gene Sequence |
| Protein sequence |
