AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013448.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013448.01.T01 MTR_8g045220 62.500 88 21 3 6 85 87 170 2.46e-27 97.8
MsG0380013448.01.T01 MTR_4g050840 97.872 47 1 0 39 85 25 71 3.42e-27 94.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013448.01.T01 ATCG00600 97.222 36 1 0 49 84 1 36 2.69e-17 68.6

Find 17 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATTGAAGTTTCTCTATT+TGG 0.115333 3:-38217457 MsG0380013448.01.T01:CDS
TCAATACAGATTCCTATTTA+AGG 0.207264 3:-38217521 MsG0380013448.01.T01:CDS
GGTAATATCTATCCTTAAAT+AGG 0.286679 3:+38217509 None:intergenic
TCGTGTCAATTGCCAATTCT+TGG 0.289545 3:-38217560 MsG0380013448.01.T01:CDS
AATAATCCAGCCAAAGTAAT+AGG 0.315680 3:+38217415 None:intergenic
AAACTTCAATCATTTGAGTT+TGG 0.328054 3:+38217467 None:intergenic
CTAATTCCTATTACTTTGGC+TGG 0.352028 3:-38217421 MsG0380013448.01.T01:CDS
TCTCTATTTGGAATCGTGTT+AGG 0.358426 3:-38217445 MsG0380013448.01.T01:CDS
AGGTCTAATTCCTATTACTT+TGG 0.388074 3:-38217425 MsG0380013448.01.T01:CDS
ATCATTTGAGTTTGGTTGAA+AGG 0.406278 3:+38217475 None:intergenic
GAATCTCAATGACCAAGAAT+TGG 0.414372 3:+38217548 None:intergenic
TTATTAATGGAATTCCTTGT+CGG 0.434610 3:-38218516 None:intergenic
TTCTTGGTCATTGAGATTCA+CGG 0.471290 3:-38217544 MsG0380013448.01.T01:CDS
AATTGGCAATTGACACGATA+AGG 0.471726 3:+38217565 None:intergenic
ATATCGACGTGGTGATCAGT+TGG 0.572503 3:-38217374 MsG0380013448.01.T01:CDS
TTTGGCTGGATTATTTGTAA+CGG 0.592623 3:-38217407 MsG0380013448.01.T01:CDS
GCATATTTACAATATCGACG+TGG 0.789819 3:-38217385 MsG0380013448.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATATAGTTACAT+AGG + Chr3:38217976-38217995 None:intergenic 10.0%
!!! ATTTTTATCTCAAAAATAAA+TGG - Chr3:38218075-38218094 MsG0380013448.01.T01:intron 10.0%
!!! TTTATTTTTGAGATAAAAAT+AGG + Chr3:38218075-38218094 None:intergenic 10.0%
!! GATTCATGAATCAATTATTA+CGG + Chr3:38218006-38218025 None:intergenic 20.0%
!! TCAAAAATAAATGGAAACTT+AGG - Chr3:38218084-38218103 MsG0380013448.01.T01:intron 20.0%
!!! TTGAGATGAATTGTAAATTT+TGG - Chr3:38218265-38218284 MsG0380013448.01.T01:intron 20.0%
!!! TTTACTAATCTCATCTTTTA+TGG + Chr3:38217605-38217624 None:intergenic 20.0%
! ACATATTCTTATACAGTCAA+AGG - Chr3:38217569-38217588 MsG0380013448.01.T01:CDS 25.0%
! ATATTCTTATACAGTCAAAG+GGG - Chr3:38217571-38217590 MsG0380013448.01.T01:CDS 25.0%
! ATGATTGAAGTTTCTCTATT+TGG - Chr3:38218419-38218438 MsG0380013448.01.T01:intron 25.0%
! CATATTCTTATACAGTCAAA+GGG - Chr3:38217570-38217589 MsG0380013448.01.T01:CDS 25.0%
! GGTAATATCTATCCTTAAAT+AGG + Chr3:38218370-38218389 None:intergenic 25.0%
! TCAATACAGATTCCTATTTA+AGG - Chr3:38218355-38218374 MsG0380013448.01.T01:intron 25.0%
! TTTCAATTGTATCTTTCAAG+TGG - Chr3:38218032-38218051 MsG0380013448.01.T01:intron 25.0%
! TTTGACTGTATAAGAATATG+TGG + Chr3:38217570-38217589 None:intergenic 25.0%
! TTTGGTTGAAAGGAAAAAAA+GGG + Chr3:38218394-38218413 None:intergenic 25.0%
!! AAACTTCAATCATTTGAGTT+TGG + Chr3:38218412-38218431 None:intergenic 25.0%
!! GATCAATAAATCTGTTGATT+TGG - Chr3:38217905-38217924 MsG0380013448.01.T01:intron 25.0%
!!! ATCTTTCAAGTGGTATTTTT+TGG - Chr3:38218042-38218061 MsG0380013448.01.T01:intron 25.0%
AATAATCCAGCCAAAGTAAT+AGG + Chr3:38218464-38218483 None:intergenic 30.0%
AGGTCTAATTCCTATTACTT+TGG - Chr3:38218451-38218470 MsG0380013448.01.T01:intron 30.0%
CTTTCTGTAAAATACTCGTT+TGG - Chr3:38217383-38217402 MsG0380013448.01.T01:CDS 30.0%
GTTTGGTTGAAAGGAAAAAA+AGG + Chr3:38218395-38218414 None:intergenic 30.0%
TAGTAGAAAACCGAAATAAC+TGG + Chr3:38218176-38218195 None:intergenic 30.0%
TATTCTTATACAGTCAAAGG+GGG - Chr3:38217572-38217591 MsG0380013448.01.T01:CDS 30.0%
TTAATTAGTCGTCCAGTAAA+AGG - Chr3:38217807-38217826 MsG0380013448.01.T01:intron 30.0%
TTATTACCAGTATTCGATAC+TGG + Chr3:38217502-38217521 None:intergenic 30.0%
TTGGTTGAAAGGAAAAAAAG+GGG + Chr3:38218393-38218412 None:intergenic 30.0%
TTTGGCTGGATTATTTGTAA+CGG - Chr3:38218469-38218488 MsG0380013448.01.T01:intron 30.0%
! AAAAAGCTCTATCCTTTTAC+TGG + Chr3:38217822-38217841 None:intergenic 30.0%
! AATAATCCAGTATCGAATAC+TGG - Chr3:38217493-38217512 MsG0380013448.01.T01:CDS 30.0%
! ATCATTTGAGTTTGGTTGAA+AGG + Chr3:38218404-38218423 None:intergenic 30.0%
AATTGGCAATTGACACGATA+AGG + Chr3:38218314-38218333 None:intergenic 35.0%
ACCGAAATAACTGGTTATAG+TGG + Chr3:38218167-38218186 None:intergenic 35.0%
CCCACTATAACCAGTTATTT+CGG - Chr3:38218163-38218182 MsG0380013448.01.T01:intron 35.0%
CCGAAATAACTGGTTATAGT+GGG + Chr3:38218166-38218185 None:intergenic 35.0%
CTAATTCCTATTACTTTGGC+TGG - Chr3:38218455-38218474 MsG0380013448.01.T01:intron 35.0%
GAATCTCAATGACCAAGAAT+TGG + Chr3:38218331-38218350 None:intergenic 35.0%
GCATATTTACAATATCGACG+TGG - Chr3:38218491-38218510 MsG0380013448.01.T01:intron 35.0%
GTATAAGAATATGTGGAGCT+CGG + Chr3:38217563-38217582 None:intergenic 35.0%
TGGTTGAAAGGAAAAAAAGG+GGG + Chr3:38218392-38218411 None:intergenic 35.0%
TTCTTGGTCATTGAGATTCA+CGG - Chr3:38218332-38218351 MsG0380013448.01.T01:intron 35.0%
! AGATCGTCAATTTTGTACCA+AGG - Chr3:38217654-38217673 MsG0380013448.01.T01:intron 35.0%
! GAAGGATAGCTATACTGATT+CGG + Chr3:38217696-38217715 None:intergenic 35.0%
! GATCGTCAATTTTGTACCAA+GGG - Chr3:38217655-38217674 MsG0380013448.01.T01:intron 35.0%
! GGTTTAGCTTACGATGTATT+TGG + Chr3:38217419-38217438 None:intergenic 35.0%
! TATTTTACAGAAAGCCGACA+AGG + Chr3:38217377-38217396 None:intergenic 35.0%
! TCTCTATTTGGAATCGTGTT+AGG - Chr3:38218431-38218450 MsG0380013448.01.T01:intron 35.0%
!! AGCAGTTTTAACAATAACCG+CGG - Chr3:38218195-38218214 MsG0380013448.01.T01:intron 35.0%
!! ATCTGTTGATTTGGAATCAC+AGG - Chr3:38217914-38217933 MsG0380013448.01.T01:intron 35.0%
!! TTGATTTGGAATCACAGGAT+GGG - Chr3:38217919-38217938 MsG0380013448.01.T01:intron 35.0%
AGCAACGCAATTTCACAATC+AGG - Chr3:38217725-38217744 MsG0380013448.01.T01:intron 40.0%
ATAACCAACCCGCAATGAAT+AGG - Chr3:38217450-38217469 MsG0380013448.01.T01:CDS 40.0%
CAACGCAATTTCACAATCAG+GGG - Chr3:38217727-38217746 MsG0380013448.01.T01:intron 40.0%
CAGACTAATAAAAAGACCCG+CGG + Chr3:38218215-38218234 None:intergenic 40.0%
GCAACGCAATTTCACAATCA+GGG - Chr3:38217726-38217745 MsG0380013448.01.T01:intron 40.0%
GGTTGAAAGGAAAAAAAGGG+GGG + Chr3:38218391-38218410 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+AGG - Chr3:38217389-38217408 MsG0380013448.01.T01:CDS 40.0%
TAACCAACCCGCAATGAATA+GGG - Chr3:38217451-38217470 MsG0380013448.01.T01:CDS 40.0%
TATACCCTCCCTATTCATTG+CGG + Chr3:38217462-38217481 None:intergenic 40.0%
TCGTGTCAATTGCCAATTCT+TGG - Chr3:38218316-38218335 MsG0380013448.01.T01:intron 40.0%
!! AACTGGTTATAGTGGGCATA+AGG + Chr3:38218159-38218178 None:intergenic 40.0%
!! GCAGTTTTAACAATAACCGC+GGG - Chr3:38218196-38218215 MsG0380013448.01.T01:intron 40.0%
!! GTTGATTTGGAATCACAGGA+TGG - Chr3:38217918-38217937 MsG0380013448.01.T01:intron 40.0%
ATACCCTCCCTATTCATTGC+GGG + Chr3:38217461-38217480 None:intergenic 45.0%
ATATCGACGTGGTGATCAGT+TGG - Chr3:38218502-38218521 MsG0380013448.01.T01:intron 45.0%
GTATACTCGAAAGAGACCCT+TGG + Chr3:38217674-38217693 None:intergenic 45.0%
TGCGTTGCTGTGTTAGAAGA+AGG + Chr3:38217714-38217733 None:intergenic 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG + Chr3:38217440-38217459 None:intergenic 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG + Chr3:38217441-38217460 None:intergenic 45.0%
CAACCCGCAATGAATAGGGA+GGG - Chr3:38217455-38217474 MsG0380013448.01.T01:CDS 50.0%
CCAACCCGCAATGAATAGGG+AGG - Chr3:38217454-38217473 MsG0380013448.01.T01:CDS 55.0%
CCTCCCTATTCATTGCGGGT+TGG + Chr3:38217457-38217476 None:intergenic 55.0%
TATGTGGAGCTCGGCATGTC+TGG + Chr3:38217554-38217573 None:intergenic 55.0%
! CTCGGCATGTCTGGAAGCAC+AGG + Chr3:38217545-38217564 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 38217366 38218532 38217366 ID=MsG0380013448.01;Name=MsG0380013448.01
Chr3 mRNA 38217366 38218532 38217366 ID=MsG0380013448.01.T01;Parent=MsG0380013448.01;Name=MsG0380013448.01.T01;_AED=0.74;_eAED=0.74;_QI=0|0|0|0.5|0|0|2|0|85
Chr3 exon 38218510 38218532 38218510 ID=MsG0380013448.01.T01:exon:1451;Parent=MsG0380013448.01.T01
Chr3 exon 38217366 38217600 38217366 ID=MsG0380013448.01.T01:exon:1450;Parent=MsG0380013448.01.T01
Chr3 CDS 38218510 38218532 38218510 ID=MsG0380013448.01.T01:cds;Parent=MsG0380013448.01.T01
Chr3 CDS 38217366 38217600 38217366 ID=MsG0380013448.01.T01:cds;Parent=MsG0380013448.01.T01
Gene Sequence

>MsG0380013448.01.T01

ATGGAATTCCTTGTCGGCTTTCTATATTCTATAGTTCCTTATCGTGTCAATTGCCAATTCTTGGTCATTGAGATTCACGGTCAATACAGATTCCTATTTAAGGATAGATATTACCCCCCTTTTTTTCCTTTCAACCAAACTCAAATGATTGAAGTTTCTCTATTTGGAATCGTGTTAGGTCTAATTCCTATTACTTTGGCTGGATTATTTGTAACGGCATATTTACAATATCGACGTGGTGATCAGTTGGATATTTGA

Protein sequence

>MsG0380013448.01.T01

MEFLVGFLYSIVPYRVNCQFLVIEIHGQYRFLFKDRYYPPFFPFNQTQMIEVSLFGIVLGLIPITLAGLFVTAYLQYRRGDQLDI*