AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001919.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001919.01.T01 MTR_8g056780 100.000 62 0 0 1 62 1 62 4.46e-36 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001919.01.T01 ATCG00300 96.774 62 2 0 1 62 1 62 5.01e-35 112

Find 9 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTACACCAATCAATAAAA+TGG 0.272913 1:-29376354 None:intergenic
GTTGTATTTGCTTCTCCTGA+TGG 0.417220 1:+29376377 MsG0180001919.01.T01:CDS
TATTTGCTTCTCCTGATGGT+TGG 0.468755 1:+29376381 MsG0180001919.01.T01:CDS
TCCGGTACATCATTATGGAT+TGG 0.474883 1:+29376428 MsG0180001919.01.T01:CDS
TTATTACTTGACCAACCATC+AGG 0.487376 1:-29376392 None:intergenic
TCCAATCCATAATGATGTAC+CGG 0.492547 1:-29376429 None:intergenic
TCAGGAGAAGCAAATACAAC+AGG 0.517061 1:-29376374 None:intergenic
ATTGGATTAGTATTTCTAGT+GGG 0.580331 1:+29376446 MsG0180001919.01.T01:CDS
GATTGGATTAGTATTTCTAG+TGG 0.595754 1:+29376445 MsG0180001919.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAAATGTTGTATTTTC+CGG + Chr1:29376410-29376429 MsG0180001919.01.T01:CDS 15.0%
!! ACTTCATCCATTTTATTGAT+TGG + Chr1:29376347-29376366 MsG0180001919.01.T01:CDS 25.0%
!!! ATTGGATTAGTATTTCTAGT+GGG + Chr1:29376446-29376465 MsG0180001919.01.T01:CDS 25.0%
AGGTACACCAATCAATAAAA+TGG - Chr1:29376357-29376376 None:intergenic 30.0%
! TATTTTCCGGTACATCATTA+TGG + Chr1:29376423-29376442 MsG0180001919.01.T01:CDS 30.0%
!! GATTGGATTAGTATTTCTAG+TGG + Chr1:29376445-29376464 MsG0180001919.01.T01:CDS 30.0%
TCCAATCCATAATGATGTAC+CGG - Chr1:29376432-29376451 None:intergenic 35.0%
TTATTACTTGACCAACCATC+AGG - Chr1:29376395-29376414 None:intergenic 35.0%
GTTGTATTTGCTTCTCCTGA+TGG + Chr1:29376377-29376396 MsG0180001919.01.T01:CDS 40.0%
TATTTGCTTCTCCTGATGGT+TGG + Chr1:29376381-29376400 MsG0180001919.01.T01:CDS 40.0%
TCAGGAGAAGCAAATACAAC+AGG - Chr1:29376377-29376396 None:intergenic 40.0%
TCCGGTACATCATTATGGAT+TGG + Chr1:29376428-29376447 MsG0180001919.01.T01:CDS 40.0%
Chromosome Type Strat End Strand Name
Chr1 gene 29376305 29376493 29376305 ID=MsG0180001919.01;Name=MsG0180001919.01
Chr1 mRNA 29376305 29376493 29376305 ID=MsG0180001919.01.T01;Parent=MsG0180001919.01;Name=MsG0180001919.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|62
Chr1 exon 29376305 29376493 29376305 ID=MsG0180001919.01.T01:exon:6722;Parent=MsG0180001919.01.T01
Chr1 CDS 29376305 29376493 29376305 ID=MsG0180001919.01.T01:cds;Parent=MsG0180001919.01.T01
Gene Sequence

>MsG0180001919.01.T01

ATGACTATTGCTTTTCAATTAGCTGTTTTTGCATTAATTGTTACTTCATCCATTTTATTGATTGGTGTACCTGTTGTATTTGCTTCTCCTGATGGTTGGTCAAGTAATAAAAATGTTGTATTTTCCGGTACATCATTATGGATTGGATTAGTATTTCTAGTGGGTATTCTTAATTCTCTCATCTCTTGA

Protein sequence

>MsG0180001919.01.T01

MTIAFQLAVFALIVTSSILLIGVPVVFASPDGWSSNKNVVFSGTSLWIGLVFLVGILNSLIS*