AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014021.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014021.01.T01 MTR_8g056780 100.000 52 0 0 1 52 1 52 4.49e-30 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014021.01.T01 ATCG00300 96.154 52 2 0 1 52 1 52 6.08e-29 97.1

Find 8 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTACACCAATCAATAAAA+TGG 0.272913 3:-48672983 None:intergenic
GTTGTATTTGCTTCTCCTGA+TGG 0.417220 3:+48673006 MsG0380014021.01.T01:CDS
TATTTGCTTCTCCTGATGGT+TGG 0.468755 3:+48673010 MsG0380014021.01.T01:CDS
TCCGGTACATCATTATGGAT+TGG 0.474883 3:+48673057 MsG0380014021.01.T01:CDS
TTATTACTTGACCAACCATC+AGG 0.487376 3:-48673021 None:intergenic
TCCAATCCATAATGATGTAC+CGG 0.492547 3:-48673058 None:intergenic
TCAGGAGAAGCAAATACAAC+AGG 0.517061 3:-48673003 None:intergenic
GGATTGGATTAGTATTCTAG+TGG 0.558154 3:+48673073 MsG0380014021.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAAATGTTGTATTTTC+CGG + Chr3:48673039-48673058 MsG0380014021.01.T01:CDS 15.0%
!! ACTTCATCCATTTTATTGAT+TGG + Chr3:48672976-48672995 MsG0380014021.01.T01:CDS 25.0%
AGGTACACCAATCAATAAAA+TGG - Chr3:48672986-48673005 None:intergenic 30.0%
! TATTTTCCGGTACATCATTA+TGG + Chr3:48673052-48673071 MsG0380014021.01.T01:CDS 30.0%
TCCAATCCATAATGATGTAC+CGG - Chr3:48673061-48673080 None:intergenic 35.0%
TTATTACTTGACCAACCATC+AGG - Chr3:48673024-48673043 None:intergenic 35.0%
GTTGTATTTGCTTCTCCTGA+TGG + Chr3:48673006-48673025 MsG0380014021.01.T01:CDS 40.0%
TATTTGCTTCTCCTGATGGT+TGG + Chr3:48673010-48673029 MsG0380014021.01.T01:CDS 40.0%
TCAGGAGAAGCAAATACAAC+AGG - Chr3:48673006-48673025 None:intergenic 40.0%
TCCGGTACATCATTATGGAT+TGG + Chr3:48673057-48673076 MsG0380014021.01.T01:CDS 40.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48672934 48673092 48672934 ID=MsG0380014021.01;Name=MsG0380014021.01
Chr3 mRNA 48672934 48673092 48672934 ID=MsG0380014021.01.T01;Parent=MsG0380014021.01;Name=MsG0380014021.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|52
Chr3 exon 48672934 48673092 48672934 ID=MsG0380014021.01.T01:exon:8590;Parent=MsG0380014021.01.T01
Chr3 CDS 48672934 48673092 48672934 ID=MsG0380014021.01.T01:cds;Parent=MsG0380014021.01.T01
Gene Sequence

>MsG0380014021.01.T01

ATGACTATTGCTTTTCAATTAGCTGTTTTTGCATTAATTGTTACTTCATCCATTTTATTGATTGGTGTACCTGTTGTATTTGCTTCTCCTGATGGTTGGTCAAGTAATAAAAATGTTGTATTTTCCGGTACATCATTATGGATTGGATTAGTATTCTAG

Protein sequence

>MsG0380014021.01.T01

MTIAFQLAVFALIVTSSILLIGVPVVFASPDGWSSNKNVVFSGTSLWIGLVF*