AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013978.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013978.01.T01 MTR_6g092730 100.000 70 0 0 1 70 1 70 1.76e-47 145
MsG0380013978.01.T01 MTR_3g035590 98.571 70 1 0 1 70 1 70 3.00e-46 141
MsG0380013978.01.T01 MTR_0002s0590 92.453 53 4 0 18 70 282 334 3.01e-30 108
MsG0380013978.01.T01 MTR_4g051210 100.000 46 0 0 25 70 77 122 1.25e-28 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013978.01.T01 ATCG00360 86.000 50 7 0 21 70 77 126 3.19e-26 93.2

Find 27 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCTCAAGTACGGTTCTA+AGG 0.265886 3:-48553741 MsG0380013978.01.T01:CDS
TTGATCAAGCCGCTGAATAT+TGG 0.308340 3:-48553640 MsG0380013978.01.T01:CDS
CTGTTCTCCGCGGTCGGAAT+AGG 0.311511 3:+48553703 None:intergenic
ATTCTGAAATTGCGGAATCT+TGG 0.383817 3:-48553664 MsG0380013978.01.T01:CDS
ATTATATTGAAGCACAGAAT+TGG 0.407853 3:-48553589 MsG0380013978.01.T01:CDS
CTATTCCGACCGCGGAGAAC+AGG 0.412079 3:-48553702 MsG0380013978.01.T01:CDS
CAATATAATTCCCAGGGGTA+AGG 0.423950 3:+48553603 None:intergenic
CGGAGAACAGGCCATTCGAC+AGG 0.449760 3:-48553690 MsG0380013978.01.T01:CDS
ACTGTTGAGCCGTATGAGTC+AGG 0.464317 3:-48553765 None:intergenic
CAGAATTGGTTGAAGATCAC+AGG 0.505448 3:-48553575 MsG0380013978.01.T01:CDS
CAAGCTATAGCCCTTACCCC+TGG 0.522286 3:-48553614 MsG0380013978.01.T01:CDS
TGTGCTTCAATATAATTCCC+AGG 0.522936 3:+48553596 None:intergenic
AATATAATTCCCAGGGGTAA+GGG 0.530205 3:+48553604 None:intergenic
ACTCTCAAGTACGGTTCTAA+GGG 0.543497 3:-48553740 MsG0380013978.01.T01:CDS
AATGGCCTGTTCTCCGCGGT+CGG 0.545774 3:+48553697 None:intergenic
GTGCTTCAATATAATTCCCA+GGG 0.548774 3:+48553597 None:intergenic
TTCAGAATCTCCCTGTCGAA+TGG 0.562027 3:+48553679 None:intergenic
AAGCTATAGCCCTTACCCCT+GGG 0.575228 3:-48553613 MsG0380013978.01.T01:CDS
AGCTTGTTTCCAATATTCAG+CGG 0.581772 3:+48553631 None:intergenic
AGTCAGGAAACTCTCAAGTA+CGG 0.591143 3:-48553749 MsG0380013978.01.T01:CDS
ACAGGGAGATTCTGAAATTG+CGG 0.592934 3:-48553672 MsG0380013978.01.T01:CDS
TCAAGTACGGTTCTAAGGGA+AGG 0.605491 3:-48553736 MsG0380013978.01.T01:CDS
GGAGAACAGGCCATTCGACA+GGG 0.615464 3:-48553689 MsG0380013978.01.T01:CDS
GTCGAATGGCCTGTTCTCCG+CGG 0.644787 3:+48553693 None:intergenic
TTACTCACCTATTCCGACCG+CGG 0.663047 3:-48553710 MsG0380013978.01.T01:CDS
AGAATTGGTTGAAGATCACA+GGG 0.667313 3:-48553574 MsG0380013978.01.T01:CDS
TGCTTCAATATAATTCCCAG+GGG 0.722159 3:+48553598 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTATATTGAAGCACAGAAT+TGG - Chr3:48553725-48553744 MsG0380013978.01.T01:CDS 25.0%
AATATAATTCCCAGGGGTAA+GGG + Chr3:48553713-48553732 None:intergenic 35.0%
AGAATTGGTTGAAGATCACA+GGG - Chr3:48553740-48553759 MsG0380013978.01.T01:CDS 35.0%
GTGCTTCAATATAATTCCCA+GGG + Chr3:48553720-48553739 None:intergenic 35.0%
TGCTTCAATATAATTCCCAG+GGG + Chr3:48553719-48553738 None:intergenic 35.0%
TGTGCTTCAATATAATTCCC+AGG + Chr3:48553721-48553740 None:intergenic 35.0%
! AGCTTGTTTCCAATATTCAG+CGG + Chr3:48553686-48553705 None:intergenic 35.0%
!! ATTCTGAAATTGCGGAATCT+TGG - Chr3:48553650-48553669 MsG0380013978.01.T01:CDS 35.0%
AACTCTCAAGTACGGTTCTA+AGG - Chr3:48553573-48553592 MsG0380013978.01.T01:CDS 40.0%
AGTCAGGAAACTCTCAAGTA+CGG - Chr3:48553565-48553584 MsG0380013978.01.T01:CDS 40.0%
CAATATAATTCCCAGGGGTA+AGG + Chr3:48553714-48553733 None:intergenic 40.0%
CAGAATTGGTTGAAGATCAC+AGG - Chr3:48553739-48553758 MsG0380013978.01.T01:CDS 40.0%
TTGATCAAGCCGCTGAATAT+TGG - Chr3:48553674-48553693 MsG0380013978.01.T01:CDS 40.0%
! ACTCTCAAGTACGGTTCTAA+GGG - Chr3:48553574-48553593 MsG0380013978.01.T01:CDS 40.0%
!! ACAGGGAGATTCTGAAATTG+CGG - Chr3:48553642-48553661 MsG0380013978.01.T01:CDS 40.0%
TTCAGAATCTCCCTGTCGAA+TGG + Chr3:48553638-48553657 None:intergenic 45.0%
!! TCAAGTACGGTTCTAAGGGA+AGG - Chr3:48553578-48553597 MsG0380013978.01.T01:CDS 45.0%
AAGCTATAGCCCTTACCCCT+GGG - Chr3:48553701-48553720 MsG0380013978.01.T01:CDS 50.0%
TTACTCACCTATTCCGACCG+CGG - Chr3:48553604-48553623 MsG0380013978.01.T01:CDS 50.0%
CAAGCTATAGCCCTTACCCC+TGG - Chr3:48553700-48553719 MsG0380013978.01.T01:CDS 55.0%
GGAGAACAGGCCATTCGACA+GGG - Chr3:48553625-48553644 MsG0380013978.01.T01:CDS 55.0%
AATGGCCTGTTCTCCGCGGT+CGG + Chr3:48553620-48553639 None:intergenic 60.0%
CGGAGAACAGGCCATTCGAC+AGG - Chr3:48553624-48553643 MsG0380013978.01.T01:CDS 60.0%
CTATTCCGACCGCGGAGAAC+AGG - Chr3:48553612-48553631 MsG0380013978.01.T01:CDS 60.0%
CTGTTCTCCGCGGTCGGAAT+AGG + Chr3:48553614-48553633 None:intergenic 60.0%
GTCGAATGGCCTGTTCTCCG+CGG + Chr3:48553624-48553643 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48553562 48553774 48553562 ID=MsG0380013978.01;Name=MsG0380013978.01
Chr3 mRNA 48553562 48553774 48553562 ID=MsG0380013978.01.T01;Parent=MsG0380013978.01;Name=MsG0380013978.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|70
Chr3 exon 48553562 48553774 48553562 ID=MsG0380013978.01.T01:exon:11322;Parent=MsG0380013978.01.T01
Chr3 CDS 48553562 48553774 48553562 ID=MsG0380013978.01.T01:cds;Parent=MsG0380013978.01.T01
Gene Sequence

>MsG0380013978.01.T01

ATGAGTCAGGAAACTCTCAAGTACGGTTCTAAGGGAAGGAATTTACTCACCTATTCCGACCGCGGAGAACAGGCCATTCGACAGGGAGATTCTGAAATTGCGGAATCTTGGTTTGATCAAGCCGCTGAATATTGGAAACAAGCTATAGCCCTTACCCCTGGGAATTATATTGAAGCACAGAATTGGTTGAAGATCACAGGGCGTTTTGAATAA

Protein sequence

>MsG0380013978.01.T01

MSQETLKYGSKGRNLLTYSDRGEQAIRQGDSEIAESWFDQAAEYWKQAIALTPGNYIEAQNWLKITGRFE*