AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049077.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049077.01.T01 MTR_8g056780 100.000 62 0 0 1 62 1 62 4.46e-36 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049077.01.T01 ATCG00300 96.774 62 2 0 1 62 1 62 5.01e-35 112

Find 9 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTACACCAATCAATAAAA+TGG 0.272913 contig65end:-1547 None:intergenic
GTTGTATTTGCTTCTCCTGA+TGG 0.417220 contig65end:+1570 MsG0080049077.01.T01:CDS
TATTTGCTTCTCCTGATGGT+TGG 0.468755 contig65end:+1574 MsG0080049077.01.T01:CDS
TCCGGTACATCATTATGGAT+TGG 0.474883 contig65end:+1621 MsG0080049077.01.T01:CDS
TTATTACTTGACCAACCATC+AGG 0.487376 contig65end:-1585 None:intergenic
TCCAATCCATAATGATGTAC+CGG 0.492547 contig65end:-1622 None:intergenic
TCAGGAGAAGCAAATACAAC+AGG 0.517061 contig65end:-1567 None:intergenic
ATTGGATTAGTATTTCTAGT+GGG 0.580331 contig65end:+1639 MsG0080049077.01.T01:CDS
GATTGGATTAGTATTTCTAG+TGG 0.595754 contig65end:+1638 MsG0080049077.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAAATGTTGTATTTTC+CGG + contig65end:1603-1622 MsG0080049077.01.T01:CDS 15.0%
!! ACTTCATCCATTTTATTGAT+TGG + contig65end:1540-1559 MsG0080049077.01.T01:CDS 25.0%
!!! ATTGGATTAGTATTTCTAGT+GGG + contig65end:1639-1658 MsG0080049077.01.T01:CDS 25.0%
AGGTACACCAATCAATAAAA+TGG - contig65end:1550-1569 None:intergenic 30.0%
! TATTTTCCGGTACATCATTA+TGG + contig65end:1616-1635 MsG0080049077.01.T01:CDS 30.0%
!! GATTGGATTAGTATTTCTAG+TGG + contig65end:1638-1657 MsG0080049077.01.T01:CDS 30.0%
TCCAATCCATAATGATGTAC+CGG - contig65end:1625-1644 None:intergenic 35.0%
TTATTACTTGACCAACCATC+AGG - contig65end:1588-1607 None:intergenic 35.0%
GTTGTATTTGCTTCTCCTGA+TGG + contig65end:1570-1589 MsG0080049077.01.T01:CDS 40.0%
TATTTGCTTCTCCTGATGGT+TGG + contig65end:1574-1593 MsG0080049077.01.T01:CDS 40.0%
TCAGGAGAAGCAAATACAAC+AGG - contig65end:1570-1589 None:intergenic 40.0%
TCCGGTACATCATTATGGAT+TGG + contig65end:1621-1640 MsG0080049077.01.T01:CDS 40.0%
Chromosome Type Strat End Strand Name
contig65end gene 1498 1686 1498 ID=MsG0080049077.01;Name=MsG0080049077.01
contig65end mRNA 1498 1686 1498 ID=MsG0080049077.01.T01;Parent=MsG0080049077.01;Name=MsG0080049077.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|0|1|0|62
contig65end exon 1498 1686 1498 ID=MsG0080049077.01.T01:exon:42233;Parent=MsG0080049077.01.T01
contig65end CDS 1498 1686 1498 ID=MsG0080049077.01.T01:cds;Parent=MsG0080049077.01.T01
Gene Sequence

>MsG0080049077.01.T01

ATGACTATTGCTTTTCAATTAGCTGTTTTTGCATTAATTGTTACTTCATCCATTTTATTGATTGGTGTACCTGTTGTATTTGCTTCTCCTGATGGTTGGTCAAGTAATAAAAATGTTGTATTTTCCGGTACATCATTATGGATTGGATTAGTATTTCTAGTGGGTATTCTTAATTCTCTCATCTCTTGA

Protein sequence

>MsG0080049077.01.T01

MTIAFQLAVFALIVTSSILLIGVPVVFASPDGWSSNKNVVFSGTSLWIGLVFLVGILNSLIS*