AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014044.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014044.01.T01 MTR_3g035710 100.000 142 0 0 24 165 1 142 5.67e-99 281
MsG0380014044.01.T01 MTR_4g051430 87.273 165 1 1 1 165 238 382 9.09e-95 280
MsG0380014044.01.T01 MTR_0002s0800 87.970 133 16 0 30 162 1 133 2.56e-82 242
MsG0380014044.01.T01 MTR_0002s0360 87.970 133 16 0 30 162 1 133 2.56e-82 242
MsG0380014044.01.T01 MTR_3g037420 92.366 131 9 1 35 165 7 136 5.12e-80 233
MsG0380014044.01.T01 MTR_8g102110 94.000 100 6 0 33 132 13 112 8.06e-60 182
MsG0380014044.01.T01 MTR_4g127750 89.189 74 8 0 1 74 67 140 2.47e-40 134
MsG0380014044.01.T01 MTR_2g015360 52.713 129 55 3 22 145 58 185 4.47e-34 119
MsG0380014044.01.T01 MTR_4g047905 97.872 47 1 0 24 70 1 47 5.26e-24 90.5
MsG0380014044.01.T01 MTR_0082s0210 88.372 43 5 0 59 101 34 76 2.84e-17 72.4
MsG0380014044.01.T01 MTR_7g076940 40.367 109 58 3 24 125 107 215 2.86e-16 73.9
MsG0380014044.01.T01 MTR_5g021800 39.815 108 58 3 24 124 107 214 6.75e-16 73.2
MsG0380014044.01.T01 MTR_8g038480 90.323 31 3 0 132 162 14 44 3.19e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014044.01.T01 ATCG00830 93.750 144 9 0 22 165 131 274 8.48e-93 271
MsG0380014044.01.T01 ATCG01310 93.750 144 9 0 22 165 131 274 8.48e-93 271
MsG0380014044.01.T01 AT2G44065 49.206 126 60 2 24 145 68 193 3.40e-32 114
MsG0380014044.01.T01 AT2G44065 49.206 126 60 2 24 145 68 193 3.40e-32 114
MsG0380014044.01.T01 AT2G44065 49.206 126 60 2 24 145 68 193 3.40e-32 114
MsG0380014044.01.T01 AT3G51190 45.872 109 52 3 23 124 107 215 1.18e-19 83.2
MsG0380014044.01.T01 AT2G18020 43.519 108 54 3 24 124 107 214 3.97e-17 76.3
MsG0380014044.01.T01 AT4G36130 40.909 110 58 3 23 125 106 215 1.06e-16 75.1

Find 48 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAGAAGCTTGTACAGTT+TGG 0.310568 3:-48721411 MsG0380014044.01.T01:CDS
GGAAGAAGCTTGTACAGTTT+GGG 0.364882 3:-48721410 MsG0380014044.01.T01:CDS
TGTTATTTCTATGTTATGTA+TGG 0.373684 3:+48721327 MsG0380014043.01.T01:CDS
CCACCATGTGGATGGTCTAC+AGG 0.374827 3:+48721067 MsG0380014043.01.T01:CDS
GTTATGTATGGCCGTGCCTA+AGG 0.403150 3:+48721339 MsG0380014043.01.T01:CDS
GCAACATTAAAATTACCTTC+TGG 0.409057 3:-48721232 MsG0380014044.01.T01:CDS
CTACTTCTTCTTCCAAGTGC+AGG 0.444988 3:+48720989 None:intergenic
GATTGCAAAAGAGGGGAAAT+CGG 0.451584 3:-48721254 MsG0380014044.01.T01:CDS
CCTTGGGGTTATCCTGCACT+TGG 0.490373 3:-48721001 MsG0380014044.01.T01:CDS
CATCCACATGGTGGTGGTGA+AGG 0.493903 3:-48721058 MsG0380014044.01.T01:CDS
AGCGAAACTGATTGCAAAAG+AGG 0.500228 3:-48721263 MsG0380014044.01.T01:CDS
ACCTAACCAACGTTTAGCTC+CGG 0.500488 3:+48721123 MsG0380014043.01.T01:CDS
GGATAACCCCAAGGAGTTGA+GGG 0.501467 3:+48721010 None:intergenic
AGCTTGTACAGTTTGGGAAG+GGG 0.511574 3:-48721404 MsG0380014044.01.T01:CDS
ACATGGTGGTGGTGAAGGGA+GGG 0.520822 3:-48721053 MsG0380014044.01.T01:CDS
CATAGAAATAACACTTGGAA+AGG 0.521855 3:-48721317 MsG0380014044.01.T01:CDS
GCCGGAGCTAAACGTTGGTT+AGG 0.522087 3:-48721124 MsG0380014044.01.T01:CDS
CACATGGTGGTGGTGAAGGG+AGG 0.525514 3:-48721054 MsG0380014044.01.T01:CDS
CTTCACCACCACCATGTGGA+TGG 0.526187 3:+48721059 MsG0380014043.01.T01:CDS
ATAACTACTCCTCTTACTAC+AGG 0.526817 3:+48721094 MsG0380014043.01.T01:CDS
CACCATGTGGATGGTCTACA+GGG 0.532724 3:+48721068 MsG0380014043.01.T01:CDS
GGTAAGCGTCCTGTAGTAAG+AGG 0.536554 3:-48721103 MsG0380014044.01.T01:CDS
ATAGAAATAACACTTGGAAA+GGG 0.539892 3:-48721316 MsG0380014044.01.T01:CDS
TCAGCAACAGTAGGACAAGT+AGG 0.541112 3:-48721181 MsG0380014044.01.T01:CDS
GGTGAAGGGAGGGCCCCGAT+TGG 0.541114 3:-48721043 MsG0380014044.01.T01:CDS
GGCCGTGCCTAAGGGCATAT+CGG 0.541386 3:+48721348 MsG0380014043.01.T01:CDS
GGACAATTAGCTAGAGCAGC+TGG 0.543739 3:-48721292 MsG0380014044.01.T01:CDS
ATCCACATGGTGGTGGTGAA+GGG 0.544348 3:-48721057 MsG0380014044.01.T01:CDS
TTATGTATGGCCGTGCCTAA+GGG 0.546055 3:+48721340 MsG0380014043.01.T01:CDS
GTAGGACAAGTAGGGAATGT+TGG 0.550877 3:-48721172 MsG0380014044.01.T01:CDS
AAGCTTGTACAGTTTGGGAA+GGG 0.552657 3:-48721405 MsG0380014044.01.T01:CDS
GCGAAACTGATTGCAAAAGA+GGG 0.554830 3:-48721262 MsG0380014044.01.T01:CDS
ATTAAAATTACCTTCTGGAG+AGG 0.560627 3:-48721227 MsG0380014044.01.T01:CDS
GAAATAACACTTGGAAAGGG+TGG 0.563823 3:-48721313 MsG0380014044.01.T01:CDS
CATAACATAGAAATAACACT+TGG 0.566932 3:-48721322 MsG0380014044.01.T01:CDS
AGGATAACCCCAAGGAGTTG+AGG 0.567413 3:+48721009 None:intergenic
AACCGATATGCCCTTAGGCA+CGG 0.569174 3:-48721350 MsG0380014044.01.T01:CDS
GAAGCTTGTACAGTTTGGGA+AGG 0.581877 3:-48721406 MsG0380014044.01.T01:CDS
CTCCCTTCACCACCACCATG+TGG 0.585580 3:+48721055 MsG0380014043.01.T01:CDS
CAGCAACAGTAGGACAAGTA+GGG 0.586255 3:-48721180 MsG0380014044.01.T01:CDS
ATCAAACGGACCTCTCCAGA+AGG 0.618237 3:+48721217 MsG0380014043.01.T01:CDS
ACTTCAACCGATATGCCCTT+AGG 0.622846 3:-48721355 MsG0380014044.01.T01:CDS
GTAGACCATCCACATGGTGG+TGG 0.631075 3:-48721064 MsG0380014044.01.T01:CDS
CGAAACTGATTGCAAAAGAG+GGG 0.648445 3:-48721261 MsG0380014044.01.T01:CDS
GCAGAGCCGGAGCTAAACGT+TGG 0.663271 3:-48721129 MsG0380014044.01.T01:CDS
CCAAGTGCAGGATAACCCCA+AGG 0.680892 3:+48721001 None:intergenic
AACCCTGTAGACCATCCACA+TGG 0.689961 3:-48721070 MsG0380014044.01.T01:CDS
CCTGTAGACCATCCACATGG+TGG 0.727826 3:-48721067 MsG0380014044.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTATTTCTATGTTATGTA+TGG + Chr3:48721025-48721044 None:intergenic 20.0%
! ATAGAAATAACACTTGGAAA+GGG - Chr3:48721033-48721052 MsG0380014044.01.T01:CDS 25.0%
! CATAACATAGAAATAACACT+TGG - Chr3:48721027-48721046 MsG0380014044.01.T01:CDS 25.0%
!!! AGCAGTTTTTTGATATCAAA+CGG + Chr3:48721149-48721168 MsG0380014043.01.T01:CDS 25.0%
CATAGAAATAACACTTGGAA+AGG - Chr3:48721032-48721051 MsG0380014044.01.T01:CDS 30.0%
GCAACATTAAAATTACCTTC+TGG - Chr3:48721117-48721136 MsG0380014044.01.T01:CDS 30.0%
GGAGTAAACCAAAAAAGTTT+GGG - Chr3:48721198-48721217 MsG0380014044.01.T01:CDS 30.0%
TGGAGTAAACCAAAAAAGTT+TGG - Chr3:48721197-48721216 MsG0380014044.01.T01:CDS 30.0%
! ATTAAAATTACCTTCTGGAG+AGG - Chr3:48721122-48721141 MsG0380014044.01.T01:CDS 30.0%
AAAAAAACCCTCAACTCCTT+GGG - Chr3:48721332-48721351 MsG0380014044.01.T01:CDS 35.0%
ATAACTACTCCTCTTACTAC+AGG + Chr3:48721258-48721277 MsG0380014043.01.T01:CDS 35.0%
!!! AGGGTTTTTTTCTACCAATC+GGG + Chr3:48721323-48721342 MsG0380014043.01.T01:CDS 35.0%
!!! GAGGGTTTTTTTCTACCAAT+CGG + Chr3:48721324-48721343 MsG0380014043.01.T01:CDS 35.0%
AAAAAACCCTCAACTCCTTG+GGG - Chr3:48721333-48721352 MsG0380014044.01.T01:CDS 40.0%
AAAAACTGCTCAGCAACAGT+AGG - Chr3:48721159-48721178 MsG0380014044.01.T01:CDS 40.0%
AAGCTTGTACAGTTTGGGAA+GGG - Chr3:48720944-48720963 MsG0380014044.01.T01:CDS 40.0%
AGCGAAACTGATTGCAAAAG+AGG - Chr3:48721086-48721105 MsG0380014044.01.T01:CDS 40.0%
CGAAACTGATTGCAAAAGAG+GGG - Chr3:48721088-48721107 MsG0380014044.01.T01:CDS 40.0%
GAAAAAAACCCTCAACTCCT+TGG - Chr3:48721331-48721350 MsG0380014044.01.T01:CDS 40.0%
GAAATAACACTTGGAAAGGG+TGG - Chr3:48721036-48721055 MsG0380014044.01.T01:CDS 40.0%
GATTGCAAAAGAGGGGAAAT+CGG - Chr3:48721095-48721114 MsG0380014044.01.T01:CDS 40.0%
GCGAAACTGATTGCAAAAGA+GGG - Chr3:48721087-48721106 MsG0380014044.01.T01:CDS 40.0%
GGAAGAAGCTTGTACAGTTT+GGG - Chr3:48720939-48720958 MsG0380014044.01.T01:CDS 40.0%
TGGAAGAAGCTTGTACAGTT+TGG - Chr3:48720938-48720957 MsG0380014044.01.T01:CDS 40.0%
!!! GGGTTTTTTTCTACCAATCG+GGG + Chr3:48721322-48721341 MsG0380014043.01.T01:CDS 40.0%
ACTTCAACCGATATGCCCTT+AGG - Chr3:48720994-48721013 MsG0380014044.01.T01:CDS 45.0%
AGCTTGTACAGTTTGGGAAG+GGG - Chr3:48720945-48720964 MsG0380014044.01.T01:CDS 45.0%
CAAAAAAGTTTGGGCAGAGC+CGG - Chr3:48721207-48721226 MsG0380014044.01.T01:CDS 45.0%
CAGCAACAGTAGGACAAGTA+GGG - Chr3:48721169-48721188 MsG0380014044.01.T01:CDS 45.0%
CTACTTCTTCTTCCAAGTGC+AGG + Chr3:48721363-48721382 MsG0380014043.01.T01:CDS 45.0%
GAAGCTTGTACAGTTTGGGA+AGG - Chr3:48720943-48720962 MsG0380014044.01.T01:CDS 45.0%
GTAGGACAAGTAGGGAATGT+TGG - Chr3:48721177-48721196 MsG0380014044.01.T01:CDS 45.0%
TCAGCAACAGTAGGACAAGT+AGG - Chr3:48721168-48721187 MsG0380014044.01.T01:CDS 45.0%
TTATGTATGGCCGTGCCTAA+GGG + Chr3:48721012-48721031 None:intergenic 45.0%
! ACCTAACCAACGTTTAGCTC+CGG + Chr3:48721229-48721248 MsG0380014043.01.T01:CDS 45.0%
AACCCTGTAGACCATCCACA+TGG - Chr3:48721279-48721298 MsG0380014044.01.T01:CDS 50.0%
AACCGATATGCCCTTAGGCA+CGG - Chr3:48720999-48721018 MsG0380014044.01.T01:CDS 50.0%
AGGATAACCCCAAGGAGTTG+AGG + Chr3:48721343-48721362 MsG0380014043.01.T01:CDS 50.0%
ATCAAACGGACCTCTCCAGA+AGG + Chr3:48721135-48721154 MsG0380014043.01.T01:CDS 50.0%
CACCATGTGGATGGTCTACA+GGG + Chr3:48721284-48721303 MsG0380014043.01.T01:CDS 50.0%
GGACAATTAGCTAGAGCAGC+TGG - Chr3:48721057-48721076 MsG0380014044.01.T01:CDS 50.0%
GGATAACCCCAAGGAGTTGA+GGG + Chr3:48721342-48721361 MsG0380014043.01.T01:CDS 50.0%
GGTAAGCGTCCTGTAGTAAG+AGG - Chr3:48721246-48721265 MsG0380014044.01.T01:CDS 50.0%
GTTATGTATGGCCGTGCCTA+AGG + Chr3:48721013-48721032 None:intergenic 50.0%
!! ATCCACATGGTGGTGGTGAA+GGG - Chr3:48721292-48721311 MsG0380014044.01.T01:CDS 50.0%
!!! CGGCTCTGCCCAAACTTTTT+TGG + Chr3:48721209-48721228 MsG0380014043.01.T01:CDS 50.0%
CCAAGTGCAGGATAACCCCA+AGG + Chr3:48721351-48721370 MsG0380014043.01.T01:CDS 55.0%
CCACCATGTGGATGGTCTAC+AGG + Chr3:48721285-48721304 MsG0380014043.01.T01:CDS 55.0%
CCTGTAGACCATCCACATGG+TGG - Chr3:48721282-48721301 MsG0380014044.01.T01:CDS 55.0%
CCTTGGGGTTATCCTGCACT+TGG - Chr3:48721348-48721367 MsG0380014044.01.T01:CDS 55.0%
CTTCACCACCACCATGTGGA+TGG + Chr3:48721293-48721312 MsG0380014043.01.T01:CDS 55.0%
GTAGACCATCCACATGGTGG+TGG - Chr3:48721285-48721304 MsG0380014044.01.T01:CDS 55.0%
! GCCGGAGCTAAACGTTGGTT+AGG - Chr3:48721225-48721244 MsG0380014044.01.T01:CDS 55.0%
!! ACATGGTGGTGGTGAAGGGA+GGG - Chr3:48721296-48721315 MsG0380014044.01.T01:CDS 55.0%
!! CATCCACATGGTGGTGGTGA+AGG - Chr3:48721291-48721310 MsG0380014044.01.T01:CDS 55.0%
CTCCCTTCACCACCACCATG+TGG + Chr3:48721297-48721316 MsG0380014043.01.T01:CDS 60.0%
GCAGAGCCGGAGCTAAACGT+TGG - Chr3:48721220-48721239 MsG0380014044.01.T01:CDS 60.0%
! GGCCGTGCCTAAGGGCATAT+CGG + Chr3:48721004-48721023 None:intergenic 60.0%
!! CACATGGTGGTGGTGAAGGG+AGG - Chr3:48721295-48721314 MsG0380014044.01.T01:CDS 60.0%
GGTGAAGGGAGGGCCCCGAT+TGG - Chr3:48721306-48721325 MsG0380014044.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48720937 48721434 48720937 ID=MsG0380014044.01;Name=MsG0380014044.01
Chr3 mRNA 48720937 48721434 48720937 ID=MsG0380014044.01.T01;Parent=MsG0380014044.01;Name=MsG0380014044.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|165
Chr3 exon 48720937 48721434 48720937 ID=MsG0380014044.01.T01:exon:9271;Parent=MsG0380014044.01.T01
Chr3 CDS 48720937 48721434 48720937 ID=MsG0380014044.01.T01:cds;Parent=MsG0380014044.01.T01
Gene Sequence

>MsG0380014044.01.T01

TTGGAAGAAGCTTGTACAGTTTGGGAAGGGGTTTTGATTGATCAAAAAGAAGAATCTACTTCAACCGATATGCCCTTAGGCACGGCCATACATAACATAGAAATAACACTTGGAAAGGGTGGACAATTAGCTAGAGCAGCTGGTGCTGTAGCGAAACTGATTGCAAAAGAGGGGAAATCGGCAACATTAAAATTACCTTCTGGAGAGGTCCGTTTGATATCAAAAAACTGCTCAGCAACAGTAGGACAAGTAGGGAATGTTGGAGTAAACCAAAAAAGTTTGGGCAGAGCCGGAGCTAAACGTTGGTTAGGTAAGCGTCCTGTAGTAAGAGGAGTAGTTATGAACCCTGTAGACCATCCACATGGTGGTGGTGAAGGGAGGGCCCCGATTGGTAGAAAAAAACCCTCAACTCCTTGGGGTTATCCTGCACTTGGAAGAAGAAGTAGAAAAAAGAATAAATATAGTGATAATTTGATTCTTCGTCGACGTAGTAAATAG

Protein sequence

>MsG0380014044.01.T01

LEEACTVWEGVLIDQKEESTSTDMPLGTAIHNIEITLGKGGQLARAAGAVAKLIAKEGKSATLKLPSGEVRLISKNCSATVGQVGNVGVNQKSLGRAGAKRWLGKRPVVRGVVMNPVDHPHGGGEGRAPIGRKKPSTPWGYPALGRRSRKKNKYSDNLILRRRSK*