AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013944.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013944.01.T01 MTR_4g034750 100.000 119 0 0 1 119 1 119 2.83e-83 239
MsG0380013944.01.T01 MTR_1779s0010 89.831 118 12 0 1 118 1 118 5.28e-75 218
MsG0380013944.01.T01 MTR_0002s0140 93.137 102 7 0 18 119 152 253 1.63e-63 194
MsG0380013944.01.T01 MTR_0002s0780 84.112 107 17 0 1 107 32 138 1.06e-62 191
MsG0380013944.01.T01 MTR_0702s0030 92.473 93 7 0 1 93 1 93 3.41e-58 175
MsG0380013944.01.T01 MTR_8g081460 92.473 93 7 0 1 93 1 93 3.41e-58 175
MsG0380013944.01.T01 MTR_2g087560 100.000 62 0 0 58 119 5 66 8.33e-39 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013944.01.T01 ATCG00790 89.916 119 12 0 1 119 17 135 2.48e-75 219
MsG0380013944.01.T01 AT2G28815 69.492 118 34 1 2 119 176 291 7.25e-52 165
MsG0380013944.01.T01 ATMG00080 44.444 117 57 1 8 116 60 176 3.98e-27 99.0

Find 40 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGTTCGTATAGGCATTT+TGG 0.204790 3:-48483370 None:intergenic
TTCGCCGAGGTGGGAAAATA+TGG 0.213680 3:+48483175 MsG0380013944.01.T01:CDS
GAAAAGGATCTCCCGAATAT+TGG 0.285746 3:+48483259 MsG0380013944.01.T01:CDS
CTTCGGAAAATATGCTCTTC+AGG 0.308187 3:+48483086 MsG0380013944.01.T01:CDS
AACGACAGCTACCCAATATT+CGG 0.319047 3:-48483271 None:intergenic
CGGCAAAATACTTTATGAAA+TGG 0.331198 3:+48483299 MsG0380013944.01.T01:CDS
TACCGAAACGCGTATGGGTT+CGG 0.335014 3:+48483236 MsG0380013944.01.T01:CDS
TCCCGAACCCATACGCGTTT+CGG 0.342652 3:-48483238 None:intergenic
TCGGTAGGTCTTACTGTAAC+AGG 0.351913 3:-48483219 None:intergenic
ACCGAAACGCGTATGGGTTC+GGG 0.378215 3:+48483237 MsG0380013944.01.T01:CDS
ACGCGTATGGGTTCGGGAAA+AGG 0.380444 3:+48483243 MsG0380013944.01.T01:CDS
AAAAGGATCTCCCGAATATT+GGG 0.401363 3:+48483260 MsG0380013944.01.T01:CDS
TTCAGGCACTTGAGCCCGCT+TGG 0.408794 3:+48483103 MsG0380013944.01.T01:CDS
CTTACTGTAACAGGTTTGTC+TGG 0.423476 3:-48483210 None:intergenic
ACGACAGCTACCCAATATTC+GGG 0.439549 3:-48483270 None:intergenic
CCCAGAAAATATAGCTAGAA+AGG 0.450098 3:+48483329 MsG0380013944.01.T01:CDS
GGCAAAATACTTTATGAAAT+GGG 0.452998 3:+48483300 MsG0380013944.01.T01:CDS
CGAGGTAATACAATTTCCTT+CGG 0.460344 3:+48483069 MsG0380013944.01.T01:CDS
AAGACCTACCGAAACGCGTA+TGG 0.469364 3:+48483230 MsG0380013944.01.T01:CDS
TCACGAAATGTTCGCCGAGG+TGG 0.471440 3:+48483165 MsG0380013944.01.T01:CDS
GGAAATTGTATTACCTCGAT+AGG 0.489800 3:-48483064 None:intergenic
GACAAATAGAAGCAGGGCGA+CGG 0.495582 3:+48483136 MsG0380013944.01.T01:CDS
CACGAAATGTTCGCCGAGGT+GGG 0.504047 3:+48483166 MsG0380013944.01.T01:CDS
AGAATGAATTGAGTTCGTAT+AGG 0.508317 3:-48483378 None:intergenic
ACATCTAGACAAATAGAAGC+AGG 0.517724 3:+48483129 MsG0380013944.01.T01:CDS
ACAAATAGAAGCAGGGCGAC+GGG 0.523315 3:+48483137 MsG0380013944.01.T01:CDS
CAACATCGAGGAAGAATGAA+AGG 0.544496 3:+48483036 None:intergenic
TCGCCGAGGTGGGAAAATAT+GGG 0.558660 3:+48483176 MsG0380013944.01.T01:CDS
AAATACTTTATGAAATGGGA+GGG 0.559811 3:+48483304 MsG0380013944.01.T01:CDS
TGTCTAGATGTGATCCAAGC+GGG 0.577171 3:-48483117 None:intergenic
AGACCTACCGAAACGCGTAT+GGG 0.590467 3:+48483231 MsG0380013944.01.T01:CDS
TGGGTAGCTGTCGTTAAACC+CGG 0.594583 3:+48483279 MsG0380013944.01.T01:CDS
AATACTTTATGAAATGGGAG+GGG 0.600055 3:+48483305 MsG0380013944.01.T01:CDS
AAAATACTTTATGAAATGGG+AGG 0.611197 3:+48483303 MsG0380013944.01.T01:CDS
GAACCCATACGCGTTTCGGT+AGG 0.634160 3:-48483234 None:intergenic
TAGAAAGGCTATTTCAATAG+CGG 0.635841 3:+48483344 MsG0380013944.01.T01:CDS
CATCTAGACAAATAGAAGCA+GGG 0.656135 3:+48483130 MsG0380013944.01.T01:CDS
ATGAAAGGAAAAGCCTATCG+AGG 0.661155 3:+48483051 MsG0380013944.01.T01:CDS
TTGTCTAGATGTGATCCAAG+CGG 0.679866 3:-48483118 None:intergenic
ATGTCACGAAATGTTCGCCG+AGG 0.702470 3:+48483162 MsG0380013944.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATACTTTATGAAATGGG+AGG + Chr3:48483303-48483322 MsG0380013944.01.T01:CDS 25.0%
! AAATACTTTATGAAATGGGA+GGG + Chr3:48483304-48483323 MsG0380013944.01.T01:CDS 25.0%
! GGCAAAATACTTTATGAAAT+GGG + Chr3:48483300-48483319 MsG0380013944.01.T01:CDS 25.0%
!!! ATTTCATAAAGTATTTTGCC+GGG - Chr3:48483300-48483319 None:intergenic 25.0%
!!! CATTTCATAAAGTATTTTGC+CGG - Chr3:48483301-48483320 None:intergenic 25.0%
AATACTTTATGAAATGGGAG+GGG + Chr3:48483305-48483324 MsG0380013944.01.T01:CDS 30.0%
AGAATGAATTGAGTTCGTAT+AGG - Chr3:48483381-48483400 None:intergenic 30.0%
CGGCAAAATACTTTATGAAA+TGG + Chr3:48483299-48483318 MsG0380013944.01.T01:CDS 30.0%
! TAGAAAGGCTATTTCAATAG+CGG + Chr3:48483344-48483363 MsG0380013944.01.T01:CDS 30.0%
!!! CCTTTCTAGCTATATTTTCT+GGG - Chr3:48483332-48483351 None:intergenic 30.0%
AAAAGGATCTCCCGAATATT+GGG + Chr3:48483260-48483279 MsG0380013944.01.T01:CDS 35.0%
ACATCTAGACAAATAGAAGC+AGG + Chr3:48483129-48483148 MsG0380013944.01.T01:CDS 35.0%
CATCTAGACAAATAGAAGCA+GGG + Chr3:48483130-48483149 MsG0380013944.01.T01:CDS 35.0%
CCCAGAAAATATAGCTAGAA+AGG + Chr3:48483329-48483348 MsG0380013944.01.T01:CDS 35.0%
CGAGGTAATACAATTTCCTT+CGG + Chr3:48483069-48483088 MsG0380013944.01.T01:CDS 35.0%
GGAAATTGTATTACCTCGAT+AGG - Chr3:48483067-48483086 None:intergenic 35.0%
! TTGAGTTCGTATAGGCATTT+TGG - Chr3:48483373-48483392 None:intergenic 35.0%
!!! GCCTTTCTAGCTATATTTTC+TGG - Chr3:48483333-48483352 None:intergenic 35.0%
AACGACAGCTACCCAATATT+CGG - Chr3:48483274-48483293 None:intergenic 40.0%
ATGAAAGGAAAAGCCTATCG+AGG + Chr3:48483051-48483070 MsG0380013944.01.T01:CDS 40.0%
CTTACTGTAACAGGTTTGTC+TGG - Chr3:48483213-48483232 None:intergenic 40.0%
CTTCGGAAAATATGCTCTTC+AGG + Chr3:48483086-48483105 MsG0380013944.01.T01:CDS 40.0%
GAAAAGGATCTCCCGAATAT+TGG + Chr3:48483259-48483278 MsG0380013944.01.T01:CDS 40.0%
TTGTCTAGATGTGATCCAAG+CGG - Chr3:48483121-48483140 None:intergenic 40.0%
!! CTGAAGAGCATATTTTCCGA+AGG - Chr3:48483088-48483107 None:intergenic 40.0%
ACGACAGCTACCCAATATTC+GGG - Chr3:48483273-48483292 None:intergenic 45.0%
TCGGTAGGTCTTACTGTAAC+AGG - Chr3:48483222-48483241 None:intergenic 45.0%
TGTCTAGATGTGATCCAAGC+GGG - Chr3:48483120-48483139 None:intergenic 45.0%
! GTACCCATATTTTCCCACCT+CGG - Chr3:48483182-48483201 None:intergenic 45.0%
AAGACCTACCGAAACGCGTA+TGG + Chr3:48483230-48483249 MsG0380013944.01.T01:CDS 50.0%
ACAAATAGAAGCAGGGCGAC+GGG + Chr3:48483137-48483156 MsG0380013944.01.T01:CDS 50.0%
AGACCTACCGAAACGCGTAT+GGG + Chr3:48483231-48483250 MsG0380013944.01.T01:CDS 50.0%
ATGTCACGAAATGTTCGCCG+AGG + Chr3:48483162-48483181 MsG0380013944.01.T01:CDS 50.0%
GACAAATAGAAGCAGGGCGA+CGG + Chr3:48483136-48483155 MsG0380013944.01.T01:CDS 50.0%
TACCGAAACGCGTATGGGTT+CGG + Chr3:48483236-48483255 MsG0380013944.01.T01:CDS 50.0%
TCGCCGAGGTGGGAAAATAT+GGG + Chr3:48483176-48483195 MsG0380013944.01.T01:CDS 50.0%
TGGGTAGCTGTCGTTAAACC+CGG + Chr3:48483279-48483298 MsG0380013944.01.T01:CDS 50.0%
TTCGCCGAGGTGGGAAAATA+TGG + Chr3:48483175-48483194 MsG0380013944.01.T01:CDS 50.0%
ACCGAAACGCGTATGGGTTC+GGG + Chr3:48483237-48483256 MsG0380013944.01.T01:CDS 55.0%
ACGCGTATGGGTTCGGGAAA+AGG + Chr3:48483243-48483262 MsG0380013944.01.T01:CDS 55.0%
CACGAAATGTTCGCCGAGGT+GGG + Chr3:48483166-48483185 MsG0380013944.01.T01:CDS 55.0%
GAACCCATACGCGTTTCGGT+AGG - Chr3:48483237-48483256 None:intergenic 55.0%
TCACGAAATGTTCGCCGAGG+TGG + Chr3:48483165-48483184 MsG0380013944.01.T01:CDS 55.0%
TCCCGAACCCATACGCGTTT+CGG - Chr3:48483241-48483260 None:intergenic 55.0%
TTCAGGCACTTGAGCCCGCT+TGG + Chr3:48483103-48483122 MsG0380013944.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48483051 48483410 48483051 ID=MsG0380013944.01;Name=MsG0380013944.01
Chr3 mRNA 48483051 48483410 48483051 ID=MsG0380013944.01.T01;Parent=MsG0380013944.01;Name=MsG0380013944.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|119
Chr3 exon 48483051 48483410 48483051 ID=MsG0380013944.01.T01:exon:17710;Parent=MsG0380013944.01.T01
Chr3 CDS 48483051 48483410 48483051 ID=MsG0380013944.01.T01:cds;Parent=MsG0380013944.01.T01
Gene Sequence

>MsG0380013944.01.T01

ATGAAAGGAAAAGCCTATCGAGGTAATACAATTTCCTTCGGAAAATATGCTCTTCAGGCACTTGAGCCCGCTTGGATCACATCTAGACAAATAGAAGCAGGGCGACGGGCAATGTCACGAAATGTTCGCCGAGGTGGGAAAATATGGGTACGCATATTTCCAGACAAACCTGTTACAGTAAGACCTACCGAAACGCGTATGGGTTCGGGAAAAGGATCTCCCGAATATTGGGTAGCTGTCGTTAAACCCGGCAAAATACTTTATGAAATGGGAGGGGTCCCAGAAAATATAGCTAGAAAGGCTATTTCAATAGCGGCATCCAAAATGCCTATACGAACTCAATTCATTCTTTCAGAATAA

Protein sequence

>MsG0380013944.01.T01

MKGKAYRGNTISFGKYALQALEPAWITSRQIEAGRRAMSRNVRRGGKIWVRIFPDKPVTVRPTETRMGSGKGSPEYWVAVVKPGKILYEMGGVPENIARKAISIAASKMPIRTQFILSE*