AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014040.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014040.01.T01 MTR_4g034750 100.000 119 0 0 1 119 1 119 2.83e-83 239
MsG0380014040.01.T01 MTR_1779s0010 89.831 118 12 0 1 118 1 118 5.28e-75 218
MsG0380014040.01.T01 MTR_0002s0140 93.137 102 7 0 18 119 152 253 1.63e-63 194
MsG0380014040.01.T01 MTR_0002s0780 84.112 107 17 0 1 107 32 138 1.06e-62 191
MsG0380014040.01.T01 MTR_0702s0030 92.473 93 7 0 1 93 1 93 3.41e-58 175
MsG0380014040.01.T01 MTR_8g081460 92.473 93 7 0 1 93 1 93 3.41e-58 175
MsG0380014040.01.T01 MTR_2g087560 100.000 62 0 0 58 119 5 66 8.33e-39 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014040.01.T01 ATCG00790 89.916 119 12 0 1 119 17 135 2.48e-75 219
MsG0380014040.01.T01 AT2G28815 69.492 118 34 1 2 119 176 291 7.25e-52 165
MsG0380014040.01.T01 ATMG00080 44.444 117 57 1 8 116 60 176 3.98e-27 99.0

Find 40 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGTTCGTATAGGCATTT+TGG 0.204790 3:+48717780 None:intergenic
TTCGCCGAGGTGGGAAAATA+TGG 0.213680 3:-48717975 MsG0380014040.01.T01:CDS
GAAAAGGATCTCCCGAATAT+TGG 0.285746 3:-48717891 MsG0380014040.01.T01:CDS
CTTCGGAAAATATGCTCTTC+AGG 0.308187 3:-48718064 MsG0380014040.01.T01:CDS
AACGACAGCTACCCAATATT+CGG 0.319047 3:+48717879 None:intergenic
CGGCAAAATACTTTATGAAA+TGG 0.331198 3:-48717851 MsG0380014040.01.T01:CDS
TACCGAAACGCGTATGGGTT+CGG 0.335014 3:-48717914 MsG0380014040.01.T01:CDS
TCCCGAACCCATACGCGTTT+CGG 0.342652 3:+48717912 None:intergenic
TCGGTAGGTCTTACTGTAAC+AGG 0.351913 3:+48717931 None:intergenic
ACCGAAACGCGTATGGGTTC+GGG 0.378215 3:-48717913 MsG0380014040.01.T01:CDS
ACGCGTATGGGTTCGGGAAA+AGG 0.380444 3:-48717907 MsG0380014040.01.T01:CDS
AAAAGGATCTCCCGAATATT+GGG 0.401363 3:-48717890 MsG0380014040.01.T01:CDS
TTCAGGCACTTGAGCCCGCT+TGG 0.408794 3:-48718047 MsG0380014040.01.T01:CDS
CTTACTGTAACAGGTTTGTC+TGG 0.423476 3:+48717940 None:intergenic
ACGACAGCTACCCAATATTC+GGG 0.439549 3:+48717880 None:intergenic
CCCAGAAAATATAGCTAGAA+AGG 0.450098 3:-48717821 MsG0380014040.01.T01:CDS
GGCAAAATACTTTATGAAAT+GGG 0.452998 3:-48717850 MsG0380014040.01.T01:CDS
CGAGGTAATACAATTTCCTT+CGG 0.460344 3:-48718081 MsG0380014040.01.T01:CDS
AAGACCTACCGAAACGCGTA+TGG 0.469364 3:-48717920 MsG0380014040.01.T01:CDS
TCACGAAATGTTCGCCGAGG+TGG 0.471440 3:-48717985 MsG0380014040.01.T01:CDS
GGAAATTGTATTACCTCGAT+AGG 0.489800 3:+48718086 None:intergenic
GACAAATAGAAGCAGGGCGA+CGG 0.495582 3:-48718014 MsG0380014040.01.T01:CDS
CACGAAATGTTCGCCGAGGT+GGG 0.504047 3:-48717984 MsG0380014040.01.T01:CDS
AGAATGAATTGAGTTCGTAT+AGG 0.508317 3:+48717772 None:intergenic
ACATCTAGACAAATAGAAGC+AGG 0.517724 3:-48718021 MsG0380014040.01.T01:CDS
ACAAATAGAAGCAGGGCGAC+GGG 0.523315 3:-48718013 MsG0380014040.01.T01:CDS
CAACATCGAGGAAGAATGAA+AGG 0.544496 3:-48718114 None:intergenic
TCGCCGAGGTGGGAAAATAT+GGG 0.558660 3:-48717974 MsG0380014040.01.T01:CDS
AAATACTTTATGAAATGGGA+GGG 0.559811 3:-48717846 MsG0380014040.01.T01:CDS
TGTCTAGATGTGATCCAAGC+GGG 0.577171 3:+48718033 None:intergenic
AGACCTACCGAAACGCGTAT+GGG 0.590467 3:-48717919 MsG0380014040.01.T01:CDS
TGGGTAGCTGTCGTTAAACC+CGG 0.594583 3:-48717871 MsG0380014040.01.T01:CDS
AATACTTTATGAAATGGGAG+GGG 0.600055 3:-48717845 MsG0380014040.01.T01:CDS
AAAATACTTTATGAAATGGG+AGG 0.611197 3:-48717847 MsG0380014040.01.T01:CDS
GAACCCATACGCGTTTCGGT+AGG 0.634160 3:+48717916 None:intergenic
TAGAAAGGCTATTTCAATAG+CGG 0.635841 3:-48717806 MsG0380014040.01.T01:CDS
CATCTAGACAAATAGAAGCA+GGG 0.656135 3:-48718020 MsG0380014040.01.T01:CDS
ATGAAAGGAAAAGCCTATCG+AGG 0.661155 3:-48718099 MsG0380014040.01.T01:CDS
TTGTCTAGATGTGATCCAAG+CGG 0.679866 3:+48718032 None:intergenic
ATGTCACGAAATGTTCGCCG+AGG 0.702470 3:-48717988 MsG0380014040.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATACTTTATGAAATGGG+AGG - Chr3:48718014-48718033 MsG0380014040.01.T01:CDS 25.0%
! AAATACTTTATGAAATGGGA+GGG - Chr3:48718015-48718034 MsG0380014040.01.T01:CDS 25.0%
! GGCAAAATACTTTATGAAAT+GGG - Chr3:48718011-48718030 MsG0380014040.01.T01:CDS 25.0%
!!! ATTTCATAAAGTATTTTGCC+GGG + Chr3:48718011-48718030 None:intergenic 25.0%
!!! CATTTCATAAAGTATTTTGC+CGG + Chr3:48718012-48718031 None:intergenic 25.0%
AATACTTTATGAAATGGGAG+GGG - Chr3:48718016-48718035 MsG0380014040.01.T01:CDS 30.0%
AGAATGAATTGAGTTCGTAT+AGG + Chr3:48718092-48718111 None:intergenic 30.0%
CGGCAAAATACTTTATGAAA+TGG - Chr3:48718010-48718029 MsG0380014040.01.T01:CDS 30.0%
! TAGAAAGGCTATTTCAATAG+CGG - Chr3:48718055-48718074 MsG0380014040.01.T01:CDS 30.0%
!!! CCTTTCTAGCTATATTTTCT+GGG + Chr3:48718043-48718062 None:intergenic 30.0%
AAAAGGATCTCCCGAATATT+GGG - Chr3:48717971-48717990 MsG0380014040.01.T01:CDS 35.0%
ACATCTAGACAAATAGAAGC+AGG - Chr3:48717840-48717859 MsG0380014040.01.T01:CDS 35.0%
CATCTAGACAAATAGAAGCA+GGG - Chr3:48717841-48717860 MsG0380014040.01.T01:CDS 35.0%
CCCAGAAAATATAGCTAGAA+AGG - Chr3:48718040-48718059 MsG0380014040.01.T01:CDS 35.0%
CGAGGTAATACAATTTCCTT+CGG - Chr3:48717780-48717799 MsG0380014040.01.T01:CDS 35.0%
GGAAATTGTATTACCTCGAT+AGG + Chr3:48717778-48717797 None:intergenic 35.0%
! TTGAGTTCGTATAGGCATTT+TGG + Chr3:48718084-48718103 None:intergenic 35.0%
!!! GCCTTTCTAGCTATATTTTC+TGG + Chr3:48718044-48718063 None:intergenic 35.0%
AACGACAGCTACCCAATATT+CGG + Chr3:48717985-48718004 None:intergenic 40.0%
ATGAAAGGAAAAGCCTATCG+AGG - Chr3:48717762-48717781 MsG0380014040.01.T01:CDS 40.0%
CTTACTGTAACAGGTTTGTC+TGG + Chr3:48717924-48717943 None:intergenic 40.0%
CTTCGGAAAATATGCTCTTC+AGG - Chr3:48717797-48717816 MsG0380014040.01.T01:CDS 40.0%
GAAAAGGATCTCCCGAATAT+TGG - Chr3:48717970-48717989 MsG0380014040.01.T01:CDS 40.0%
TTGTCTAGATGTGATCCAAG+CGG + Chr3:48717832-48717851 None:intergenic 40.0%
!! CTGAAGAGCATATTTTCCGA+AGG + Chr3:48717799-48717818 None:intergenic 40.0%
ACGACAGCTACCCAATATTC+GGG + Chr3:48717984-48718003 None:intergenic 45.0%
TCGGTAGGTCTTACTGTAAC+AGG + Chr3:48717933-48717952 None:intergenic 45.0%
TGTCTAGATGTGATCCAAGC+GGG + Chr3:48717831-48717850 None:intergenic 45.0%
! GTACCCATATTTTCCCACCT+CGG + Chr3:48717893-48717912 None:intergenic 45.0%
AAGACCTACCGAAACGCGTA+TGG - Chr3:48717941-48717960 MsG0380014040.01.T01:CDS 50.0%
ACAAATAGAAGCAGGGCGAC+GGG - Chr3:48717848-48717867 MsG0380014040.01.T01:CDS 50.0%
AGACCTACCGAAACGCGTAT+GGG - Chr3:48717942-48717961 MsG0380014040.01.T01:CDS 50.0%
ATGTCACGAAATGTTCGCCG+AGG - Chr3:48717873-48717892 MsG0380014040.01.T01:CDS 50.0%
GACAAATAGAAGCAGGGCGA+CGG - Chr3:48717847-48717866 MsG0380014040.01.T01:CDS 50.0%
TACCGAAACGCGTATGGGTT+CGG - Chr3:48717947-48717966 MsG0380014040.01.T01:CDS 50.0%
TCGCCGAGGTGGGAAAATAT+GGG - Chr3:48717887-48717906 MsG0380014040.01.T01:CDS 50.0%
TGGGTAGCTGTCGTTAAACC+CGG - Chr3:48717990-48718009 MsG0380014040.01.T01:CDS 50.0%
TTCGCCGAGGTGGGAAAATA+TGG - Chr3:48717886-48717905 MsG0380014040.01.T01:CDS 50.0%
ACCGAAACGCGTATGGGTTC+GGG - Chr3:48717948-48717967 MsG0380014040.01.T01:CDS 55.0%
ACGCGTATGGGTTCGGGAAA+AGG - Chr3:48717954-48717973 MsG0380014040.01.T01:CDS 55.0%
CACGAAATGTTCGCCGAGGT+GGG - Chr3:48717877-48717896 MsG0380014040.01.T01:CDS 55.0%
GAACCCATACGCGTTTCGGT+AGG + Chr3:48717948-48717967 None:intergenic 55.0%
TCACGAAATGTTCGCCGAGG+TGG - Chr3:48717876-48717895 MsG0380014040.01.T01:CDS 55.0%
TCCCGAACCCATACGCGTTT+CGG + Chr3:48717952-48717971 None:intergenic 55.0%
TTCAGGCACTTGAGCCCGCT+TGG - Chr3:48717814-48717833 MsG0380014040.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48717762 48718121 48717762 ID=MsG0380014040.01;Name=MsG0380014040.01
Chr3 mRNA 48717762 48718121 48717762 ID=MsG0380014040.01.T01;Parent=MsG0380014040.01;Name=MsG0380014040.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|119
Chr3 exon 48717762 48718121 48717762 ID=MsG0380014040.01.T01:exon:7354;Parent=MsG0380014040.01.T01
Chr3 CDS 48717762 48718121 48717762 ID=MsG0380014040.01.T01:cds;Parent=MsG0380014040.01.T01
Gene Sequence

>MsG0380014040.01.T01

ATGAAAGGAAAAGCCTATCGAGGTAATACAATTTCCTTCGGAAAATATGCTCTTCAGGCACTTGAGCCCGCTTGGATCACATCTAGACAAATAGAAGCAGGGCGACGGGCAATGTCACGAAATGTTCGCCGAGGTGGGAAAATATGGGTACGCATATTTCCAGACAAACCTGTTACAGTAAGACCTACCGAAACGCGTATGGGTTCGGGAAAAGGATCTCCCGAATATTGGGTAGCTGTCGTTAAACCCGGCAAAATACTTTATGAAATGGGAGGGGTCCCAGAAAATATAGCTAGAAAGGCTATTTCAATAGCGGCATCCAAAATGCCTATACGAACTCAATTCATTCTTTCAGAATAA

Protein sequence

>MsG0380014040.01.T01

MKGKAYRGNTISFGKYALQALEPAWITSRQIEAGRRAMSRNVRRGGKIWVRIFPDKPVTVRPTETRMGSGKGSPEYWVAVVKPGKILYEMGGVPENIARKAISIAASKMPIRTQFILSE*