AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019279.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019279.01.T01 MTR_4g028180 94.444 126 6 1 1 125 1 126 1.72e-82 238
MsG0480019279.01.T01 MTR_7g073170 46.667 120 58 2 4 123 3 116 2.74e-32 110
MsG0480019279.01.T01 MTR_7g073150 45.455 121 61 2 4 124 3 118 3.34e-30 105
MsG0480019279.01.T01 MTR_4g428370 48.673 113 52 3 9 121 62 168 1.53e-28 102
MsG0480019279.01.T01 MTR_4g028190 40.323 124 70 1 1 124 1 120 1.78e-27 98.6
MsG0480019279.01.T01 MTR_7g072930 43.548 124 64 3 4 126 3 121 1.80e-26 96.7
MsG0480019279.01.T01 MTR_4g027800 44.248 113 57 3 9 121 5 111 1.15e-25 93.6
MsG0480019279.01.T01 MTR_4g027840 44.248 113 57 3 9 121 5 111 5.29e-25 92.8
MsG0480019279.01.T01 MTR_7g072900 42.500 120 64 2 4 123 3 117 8.05e-24 89.0
MsG0480019279.01.T01 MTR_7g072993 44.715 123 61 3 4 124 3 120 1.15e-23 89.4
MsG0480019279.01.T01 MTR_2g062600 37.815 119 67 3 6 123 1 113 3.17e-23 87.4
MsG0480019279.01.T01 MTR_7g073130 42.857 126 61 4 4 126 3 120 4.09e-23 87.4
MsG0480019279.01.T01 MTR_1g040335 44.715 123 61 3 4 125 3 119 5.45e-23 87.0
MsG0480019279.01.T01 MTR_7g072830 39.024 123 69 3 4 125 3 120 6.51e-23 86.7
MsG0480019279.01.T01 MTR_4g029210 38.983 118 66 3 9 126 5 116 3.64e-22 84.7
MsG0480019279.01.T01 MTR_4g029350 39.837 123 67 3 4 126 2 117 3.85e-22 84.7
MsG0480019279.01.T01 MTR_7g072730 37.705 122 70 3 4 124 3 119 1.59e-21 84.3
MsG0480019279.01.T01 MTR_4g029390 39.837 123 67 3 4 126 2 117 8.86e-21 81.3
MsG0480019279.01.T01 MTR_7g073120 38.889 126 69 4 1 124 1 120 1.02e-20 82.0
MsG0480019279.01.T01 MTR_7g072980 37.705 122 70 3 4 124 3 119 1.95e-20 80.9
MsG0480019279.01.T01 MTR_7g073060 36.885 122 72 2 4 125 3 119 6.22e-20 79.0
MsG0480019279.01.T01 MTR_7g072810 40.984 122 60 4 4 124 3 113 1.40e-19 78.6
MsG0480019279.01.T01 MTR_7g073100 37.190 121 71 2 4 124 3 118 1.75e-19 79.0
MsG0480019279.01.T01 MTR_7g072960 38.710 124 56 3 4 126 3 107 2.67e-19 77.8
MsG0480019279.01.T01 MTR_7g072760 37.903 124 71 3 4 125 3 122 2.47e-16 70.1
MsG0480019279.01.T01 MTR_7g073030 35.537 121 73 2 4 124 3 118 1.10e-15 69.3
MsG0480019279.01.T01 MTR_7g451500 31.183 93 60 1 31 123 9 97 1.09e-14 65.1
MsG0480019279.01.T01 MTR_3g046515 29.508 122 80 2 2 122 3 119 2.47e-13 62.4
MsG0480019279.01.T01 MTR_3g467100 29.839 124 74 5 1 123 1 112 7.10e-13 60.8
MsG0480019279.01.T01 MTR_3g056640 32.456 114 71 2 10 122 11 119 1.74e-12 60.1
MsG0480019279.01.T01 MTR_1g040330 35.537 121 71 3 4 123 3 117 1.89e-12 60.1
MsG0480019279.01.T01 MTR_3g046530 28.689 122 81 2 2 122 3 119 3.38e-12 59.3
MsG0480019279.01.T01 MTR_3g046540 28.689 122 81 2 2 122 3 119 4.75e-12 58.9
MsG0480019279.01.T01 MTR_4g028250 33.613 119 72 4 9 125 5 118 1.69e-11 57.4
MsG0480019279.01.T01 MTR_3g464130 34.409 93 55 2 31 122 19 106 5.05e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019279.01.T01 AT2G15050 52.101 119 51 3 9 124 6 121 2.73e-32 110
MsG0480019279.01.T01 AT2G15050 52.137 117 50 3 9 122 6 119 1.11e-31 109
MsG0480019279.01.T01 AT2G15050 52.252 111 47 3 9 116 6 113 1.05e-29 103
MsG0480019279.01.T01 AT2G38540 44.737 114 59 2 9 122 6 115 1.02e-28 101
MsG0480019279.01.T01 AT5G01870 44.643 112 56 4 11 122 8 113 2.85e-27 97.8
MsG0480019279.01.T01 AT3G08770 48.980 98 46 3 25 122 17 110 1.14e-25 93.6
MsG0480019279.01.T01 AT3G08770 48.980 98 46 3 25 122 17 110 1.28e-25 93.6
MsG0480019279.01.T01 AT5G59320 48.148 108 50 3 15 122 11 112 5.81e-25 92.0
MsG0480019279.01.T01 AT5G59310 48.113 106 43 4 16 121 15 108 6.67e-24 89.0
MsG0480019279.01.T01 AT2G38530 43.860 114 60 2 9 122 6 115 2.96e-23 87.8
MsG0480019279.01.T01 AT3G51600 43.860 114 60 2 9 122 6 115 1.81e-22 85.5
MsG0480019279.01.T01 AT3G51590 40.351 114 62 3 9 122 6 113 1.45e-21 83.2
MsG0480019279.01.T01 AT2G18370 35.088 114 67 2 10 121 4 112 1.29e-18 75.9
MsG0480019279.01.T01 AT4G33355 35.000 120 71 3 4 123 5 117 7.77e-15 66.2
MsG0480019279.01.T01 AT4G33355 34.454 119 71 3 4 122 5 116 7.95e-14 63.5

Find 32 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTTGAGCCATAGTATTA+AGG 0.282339 4:+17657638 None:intergenic
GAAGACCAGCAGCAAGATTA+AGG 0.290840 4:+17657539 None:intergenic
GGCAACTAAGCTTGTTTGTT+TGG 0.318434 4:-17657809 MsG0480019279.01.T01:CDS
ATTGTGTTTCTTGTAACTTT+TGG 0.338311 4:-17657784 MsG0480019279.01.T01:CDS
GCCCTAATACCGACTGTAAC+AGG 0.378156 4:-17657480 MsG0480019279.01.T01:CDS
TACCTGTTACAGTCGGTATT+AGG 0.379758 4:+17657478 None:intergenic
GGGTATAAGCTTGTCACAAC+TGG 0.381388 4:+17657727 None:intergenic
ACCTGTTACAGTCGGTATTA+GGG 0.395247 4:+17657479 None:intergenic
CATCATGGGTTACTCAACTA+TGG 0.397979 4:-17657830 None:intergenic
GTAACAGCTGCTTCAGCCTT+AGG 0.410392 4:+17657760 None:intergenic
AGTAAGGAACCTTAATACTA+TGG 0.425103 4:-17657647 MsG0480019279.01.T01:CDS
GCTAACAGCATTCTTAATAC+AGG 0.426517 4:+17657591 None:intergenic
AACAGCTCTACGATCATTTG+TGG 0.431202 4:+17657618 None:intergenic
TTAAACCTTAATCTTGCTGC+TGG 0.432503 4:-17657544 MsG0480019279.01.T01:CDS
AGTCGGTATTAGGGCTGATC+TGG 0.443403 4:+17657488 None:intergenic
TGTGTTTCTTACATTATGAC+TGG 0.454062 4:-17657703 MsG0480019279.01.T01:CDS
CACAGTGTTGTAATGGAGTA+AGG 0.467403 4:-17657663 MsG0480019279.01.T01:CDS
AAGAATGCTGTTAGCCAAAG+TGG 0.478268 4:-17657583 MsG0480019279.01.T01:CDS
GTTCCAGCACAGTGTTGTAA+TGG 0.512359 4:-17657670 MsG0480019279.01.T01:CDS
ATGTTAACACCACATTTCCT+TGG 0.522311 4:+17657517 None:intergenic
GTATTAGGGCTGATCTGGTA+AGG 0.526182 4:+17657493 None:intergenic
ACTCCATTACAACACTGTGC+TGG 0.541228 4:+17657667 None:intergenic
GCTTGTCACAACTGGACCAC+AGG 0.541996 4:+17657735 None:intergenic
ATCTTGCTGCTGGTCTTCCA+AGG 0.551966 4:-17657534 MsG0480019279.01.T01:CDS
ACATATGTGAATATTATAAG+TGG 0.553344 4:+17657455 None:intergenic
TAGTGTAACTGAAACCACTT+TGG 0.557954 4:+17657569 None:intergenic
GTTTCTTACATTATGACTGG+TGG 0.579604 4:-17657700 MsG0480019279.01.T01:CDS
GCTGGTCTTCCAAGGAAATG+TGG 0.584199 4:-17657526 MsG0480019279.01.T01:CDS
GAAGCAGCTGTTACATCCTG+TGG 0.593294 4:-17657751 MsG0480019279.01.T01:CDS
GTCATAATGTAAGAAACACA+AGG 0.653065 4:+17657706 None:intergenic
TAAGTGGTACCTGTTACAGT+CGG 0.691959 4:+17657471 None:intergenic
TCATAATGTAAGAAACACAA+GGG 0.708823 4:+17657707 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCATAATGTAAGAAACACAA+GGG + Chr4:17657590-17657609 None:intergenic 25.0%
!! ATTGTGTTTCTTGTAACTTT+TGG - Chr4:17657510-17657529 MsG0480019279.01.T01:CDS 25.0%
AGTAAGGAACCTTAATACTA+TGG - Chr4:17657647-17657666 MsG0480019279.01.T01:CDS 30.0%
GTCATAATGTAAGAAACACA+AGG + Chr4:17657591-17657610 None:intergenic 30.0%
TGTGTTTCTTACATTATGAC+TGG - Chr4:17657591-17657610 MsG0480019279.01.T01:CDS 30.0%
ATGTTAACACCACATTTCCT+TGG + Chr4:17657780-17657799 None:intergenic 35.0%
GCTAACAGCATTCTTAATAC+AGG + Chr4:17657706-17657725 None:intergenic 35.0%
GTTTCTTACATTATGACTGG+TGG - Chr4:17657594-17657613 MsG0480019279.01.T01:CDS 35.0%
TAGTGTAACTGAAACCACTT+TGG + Chr4:17657728-17657747 None:intergenic 35.0%
TGGTTTGAGCCATAGTATTA+AGG + Chr4:17657659-17657678 None:intergenic 35.0%
TTAAACCTTAATCTTGCTGC+TGG - Chr4:17657750-17657769 MsG0480019279.01.T01:CDS 35.0%
! TCTTGTAACTTTTGGTCCTA+AGG - Chr4:17657518-17657537 MsG0480019279.01.T01:CDS 35.0%
AACAGCTCTACGATCATTTG+TGG + Chr4:17657679-17657698 None:intergenic 40.0%
AAGAATGCTGTTAGCCAAAG+TGG - Chr4:17657711-17657730 MsG0480019279.01.T01:CDS 40.0%
ACCTGTTACAGTCGGTATTA+GGG + Chr4:17657818-17657837 None:intergenic 40.0%
TAAGTGGTACCTGTTACAGT+CGG + Chr4:17657826-17657845 None:intergenic 40.0%
TACCTGTTACAGTCGGTATT+AGG + Chr4:17657819-17657838 None:intergenic 40.0%
! CACAGTGTTGTAATGGAGTA+AGG - Chr4:17657631-17657650 MsG0480019279.01.T01:CDS 40.0%
! GGCAACTAAGCTTGTTTGTT+TGG - Chr4:17657485-17657504 MsG0480019279.01.T01:CDS 40.0%
ACTCCATTACAACACTGTGC+TGG + Chr4:17657630-17657649 None:intergenic 45.0%
GAAGACCAGCAGCAAGATTA+AGG + Chr4:17657758-17657777 None:intergenic 45.0%
GGGTATAAGCTTGTCACAAC+TGG + Chr4:17657570-17657589 None:intergenic 45.0%
GTATTAGGGCTGATCTGGTA+AGG + Chr4:17657804-17657823 None:intergenic 45.0%
GTTCCAGCACAGTGTTGTAA+TGG - Chr4:17657624-17657643 MsG0480019279.01.T01:CDS 45.0%
AGTCGGTATTAGGGCTGATC+TGG + Chr4:17657809-17657828 None:intergenic 50.0%
GAAGCAGCTGTTACATCCTG+TGG - Chr4:17657543-17657562 MsG0480019279.01.T01:CDS 50.0%
GTAACAGCTGCTTCAGCCTT+AGG + Chr4:17657537-17657556 None:intergenic 50.0%
! GCCCTAATACCGACTGTAAC+AGG - Chr4:17657814-17657833 MsG0480019279.01.T01:CDS 50.0%
!! ATCTTGCTGCTGGTCTTCCA+AGG - Chr4:17657760-17657779 MsG0480019279.01.T01:CDS 50.0%
!! GCTGGTCTTCCAAGGAAATG+TGG - Chr4:17657768-17657787 MsG0480019279.01.T01:CDS 50.0%
GCTTGTCACAACTGGACCAC+AGG + Chr4:17657562-17657581 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 17657468 17657848 17657468 ID=MsG0480019279.01;Name=MsG0480019279.01
Chr4 mRNA 17657468 17657848 17657468 ID=MsG0480019279.01.T01;Parent=MsG0480019279.01;Name=MsG0480019279.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|126
Chr4 exon 17657468 17657848 17657468 ID=MsG0480019279.01.T01:exon:5481;Parent=MsG0480019279.01.T01
Chr4 CDS 17657468 17657848 17657468 ID=MsG0480019279.01.T01:cds;Parent=MsG0480019279.01.T01
Gene Sequence

>MsG0480019279.01.T01

ATGGGTTACTCAACTATGGCAACTAAGCTTGTTTGTTTGGCGATTGTGTTTCTTGTAACTTTTGGTCCTAAGGCTGAAGCAGCTGTTACATCCTGTGGTCCAGTTGTGACAAGCTTATACCCTTGTGTTTCTTACATTATGACTGGTGGAAACACTGTTCCAGCACAGTGTTGTAATGGAGTAAGGAACCTTAATACTATGGCTCAAACCACAAATGATCGTAGAGCTGTTTGTACCTGTATTAAGAATGCTGTTAGCCAAAGTGGTTTCAGTTACACTAATTTAAACCTTAATCTTGCTGCTGGTCTTCCAAGGAAATGTGGTGTTAACATTCCTTACCAGATCAGCCCTAATACCGACTGTAACAGGTACCACTTATAA

Protein sequence

>MsG0480019279.01.T01

MGYSTMATKLVCLAIVFLVTFGPKAEAAVTSCGPVVTSLYPCVSYIMTGGNTVPAQCCNGVRNLNTMAQTTNDRRAVCTCIKNAVSQSGFSYTNLNLNLAAGLPRKCGVNIPYQISPNTDCNRYHL*