AlfalfaGEDB | MsG0180004994.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004994.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004994.01.T01	XP_013469395.1	77.551	98	5	2	1	81	1	98	3.90E-40	139

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004994.01.T01	Q1S9I9	66.055	109	9	3	1	81	1	109	8.11E-36	122

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004994.01.T01	I3S6Z4	77.551	98	5	2	1	81	1	98	1.86e-40	139

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004120.01	MsG0180004994.01	0.822681	2.079495e-53	1.752713e-50
MsG0180004538.01	MsG0180004994.01	0.831639	1.489625e-55	1.626209e-52
MsG0180004583.01	MsG0180004994.01	0.820066	8.346428e-53	6.536904e-50
MsG0180004994.01	MsG0180004995.01	0.836464	9.218032e-57	1.162581e-53
MsG0180004994.01	MsG0180005048.01	0.859378	4.312686e-63	1.136741e-59
MsG0180004994.01	MsG0180006133.01	0.847218	1.329003e-59	2.345467e-56
MsG0180004994.01	MsG0380014666.01	0.851512	8.456032e-61	1.715603e-57
MsG0180004994.01	MsG0380015090.01	0.810444	1.149300e-50	6.928803e-48
MsG0180004994.01	MsG0380015219.01	0.801601	8.335834e-49	3.997106e-46
MsG0180004994.01	MsG0380015340.01	0.800180	1.626599e-48	7.524306e-46
MsG0180004994.01	MsG0380016027.01	0.833553	4.991873e-56	5.768801e-53
MsG0180004994.01	MsG0380016225.01	0.816986	4.167661e-52	2.997997e-49
MsG0180004994.01	MsG0380017282.01	0.807888	4.055686e-50	2.285509e-47
MsG0180004994.01	MsG0480018106.01	0.820534	6.519580e-53	5.172275e-50
MsG0180004994.01	MsG0480018785.01	0.810925	9.046117e-51	5.524602e-48
MsG0180004994.01	MsG0480020398.01	0.806617	7.538973e-50	4.110585e-47
MsG0180004994.01	MsG0480020856.01	0.802137	6.470887e-49	3.145517e-46
MsG0180004994.01	MsG0480020918.01	0.811002	8.702004e-51	5.325723e-48
MsG0180004994.01	MsG0480020921.01	0.808815	2.572933e-50	1.485693e-47
MsG0180004994.01	MsG0480020926.01	0.821177	4.639079e-53	3.746931e-50
MsG0180004994.01	MsG0480020971.01	0.802847	4.618721e-49	2.286314e-46
MsG0180004994.01	MsG0480021015.01	0.833310	5.740732e-56	6.586070e-53
MsG0180004994.01	MsG0480021100.01	0.804305	2.302845e-49	1.183290e-46
MsG0180004994.01	MsG0480021301.01	0.853083	3.020195e-61	6.448164e-58
MsG0180004994.01	MsG0480021302.01	0.820043	8.449810e-53	6.613629e-50
MsG0180004994.01	MsG0480021902.01	0.814779	1.294934e-51	8.768949e-49
MsG0180004994.01	MsG0480022307.01	0.830479	2.869895e-55	3.027078e-52
MsG0180004994.01	MsG0480022784.01	0.804167	2.460157e-49	1.259638e-46
MsG0180004994.01	MsG0480023003.01	0.825356	4.902300e-54	4.457764e-51
MsG0180004994.01	MsG0480023424.01	0.848158	7.326574e-60	1.332246e-56
MsG0180004994.01	MsG0480023426.01	0.839496	1.530940e-57	2.117989e-54
MsG0180004994.01	MsG0480023428.01	0.823285	1.503826e-53	1.289230e-50
MsG0180004994.01	MsG0480023653.01	0.802396	5.724199e-49	2.801076e-46
MsG0180004994.01	MsG0480023706.01	0.808618	2.835505e-50	1.628798e-47
MsG0180004994.01	MsG0580024787.01	0.818690	1.718537e-52	1.295330e-49
MsG0180004994.01	MsG0580025177.01	0.805147	1.536391e-49	8.067813e-47
MsG0180004994.01	MsG0580025203.01	0.830009	3.738550e-55	3.890201e-52
MsG0180004994.01	MsG0580025504.01	0.847124	1.410125e-59	2.481566e-56
MsG0180004994.01	MsG0580025506.01	0.835349	1.766713e-56	2.154921e-53
MsG0180004994.01	MsG0580028927.01	0.802148	6.436601e-49	3.129763e-46
MsG0180004994.01	MsG0580028955.01	0.830976	2.168270e-55	2.320917e-52
MsG0180004994.01	MsG0580029591.01	0.802530	5.369586e-49	2.636401e-46
MsG0180004994.01	MsG0780036534.01	0.808930	2.430988e-50	1.407989e-47
MsG0180004994.01	MsG0780040620.01	0.852696	3.896326e-61	8.209657e-58
MsG0180004994.01	MsG0780040641.01	0.816935	4.278055e-52	3.073052e-49
MsG0180004994.01	MsG0780041273.01	0.856226	3.713087e-62	8.799649e-59
MsG0180004994.01	MsG0780041359.01	0.817791	2.745543e-52	2.018944e-49
MsG0180004994.01	MsG0780041546.01	0.828093	1.088887e-54	1.071521e-51
MsG0180004994.01	MsG0880045015.01	0.842676	2.235455e-58	3.414595e-55
MsG0180004994.01	MsG0880045647.01	0.815376	9.545124e-52	6.569554e-49
MsG0180004994.01	MsG0880046904.01	0.802167	6.379222e-49	3.103372e-46
MsG0180004994.01	MsG0880046905.01	0.816013	6.882709e-52	4.820732e-49
MsG0180004994.01	MsG0880047649.01	0.817109	3.909763e-52	2.821720e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004994.01.T01	MTR_1g492910	77.551	98	5	2	1	81	1	98	4.72e-44	139
MsG0180004994.01.T01	MTR_1g090780	77.551	98	5	2	1	81	1	98	5.44e-44	139
MsG0180004994.01.T01	MTR_4g063240	66.667	108	9	3	1	81	1	108	3.12e-38	125
MsG0180004994.01.T01	MTR_4g063220	65.138	109	10	3	1	81	1	109	7.00e-37	122
MsG0180004994.01.T01	MTR_4g063990	65.138	109	10	3	1	81	1	109	7.00e-37	122
MsG0180004994.01.T01	MTR_4g063200	65.138	109	10	3	1	81	1	109	7.00e-37	122
MsG0180004994.01.T01	MTR_4g064020	65.138	109	10	3	1	81	1	109	7.00e-37	122
MsG0180004994.01.T01	MTR_4g071180	66.055	109	9	3	1	81	1	109	7.90e-37	122
MsG0180004994.01.T01	MTR_4g070240	66.055	109	9	3	1	81	1	109	7.90e-37	122
MsG0180004994.01.T01	MTR_4g088025	67.308	104	11	3	1	81	1	104	1.62e-36	120
MsG0180004994.01.T01	MTR_4g070245	65.138	109	10	3	1	81	1	109	4.02e-36	120
MsG0180004994.01.T01	MTR_4g064983	65.138	109	10	3	1	81	1	109	7.45e-36	122
MsG0180004994.01.T01	MTR_4g063275	63.303	109	12	3	1	81	1	109	2.81e-35	117
MsG0180004994.01.T01	MTR_7g099960	94.340	53	2	1	30	81	47	99	2.02e-29	102
MsG0180004994.01.T01	MTR_1g068600	90.566	53	4	1	30	81	45	97	6.93e-29	101
MsG0180004994.01.T01	MTR_8g069990	61.364	88	25	2	1	79	1	88	3.37e-27	95.9
MsG0180004994.01.T01	MTR_2g084480	47.170	53	27	1	32	83	136	188	6.53e-13	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004994.01.T01	AT3G53650	71.717	99	10	4	1	81	1	99	2.62e-36	120
MsG0180004994.01.T01	AT2G37470	70.000	100	11	4	1	81	1	100	7.98e-36	119
MsG0180004994.01.T01	AT2G28720	59.821	112	14	2	1	81	1	112	1.16e-34	116
MsG0180004994.01.T01	AT3G45980	61.261	111	13	3	1	81	1	111	3.92e-34	115
MsG0180004994.01.T01	AT1G07790	61.468	109	14	3	1	81	1	109	1.90e-33	113
MsG0180004994.01.T01	AT5G59910	65.957	94	12	3	8	81	18	111	4.85e-31	107
MsG0180004994.01.T01	AT3G46030	68.966	87	18	3	4	81	20	106	4.45e-30	104
MsG0180004994.01.T01	AT5G02570	65.263	95	17	3	1	81	1	93	6.79e-30	103
MsG0180004994.01.T01	AT5G22880	59.615	104	16	3	4	81	3	106	7.64e-29	101
MsG0180004994.01.T01	AT3G09480	63.218	87	26	3	1	81	1	87	5.86e-26	93.6
MsG0180004994.01.T01	AT1G08170	48.980	49	24	1	34	81	151	199	1.67e-13	63.9

Find 29 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTCAAGAGCTAGTGAATT+TGG	0.219657	1:-85374764	None:intergenic
TGCCGTCGTCTTGTTCTTCC+TGG	0.266555	1:+85374698	MsG0180004994.01.T01:CDS
CTTGAAGATGTAGATCTTGT+AGG	0.279657	1:-85374521	None:intergenic
TCAGCTGGTGCCTTCTCTGC+AGG	0.357925	1:-85374420	None:intergenic
ACGGGTTTCTTCTCTCCCTT+TGG	0.367004	1:-85374387	None:intergenic
CTCTGCAGGCTTCTTCTCTA+CGG	0.367054	1:-85374406	None:intergenic
AGTGAATTTGGTGACAGCTT+TGG	0.420989	1:-85374752	None:intergenic
CTACAAGATCTACATCTTCA+AGG	0.431521	1:+85374522	MsG0180004994.01.T01:CDS
GCCTTGTGAGATACGATGTC+AGG	0.445325	1:-85374562	None:intergenic
TCTGCAGGCTTCTTCTCTAC+GGG	0.452138	1:-85374405	None:intergenic
ATCGTATCTCACAAGGCTAT+GGG	0.459801	1:+85374568	MsG0180004994.01.T01:CDS
CATCGTATCTCACAAGGCTA+TGG	0.470354	1:+85374567	MsG0180004994.01.T01:CDS
CATCTTCAAGGTTCTCAAGC+AGG	0.470454	1:+85374534	MsG0180004994.01.T01:CDS
GGTGACAGCTTTGGTTCCCT+CGG	0.503972	1:-85374743	None:intergenic
CTGAATCTCCCGAGAGGTGA+TGG	0.509224	1:-85374673	None:intergenic
AGAGAAGAAGCCTGCAGAGA+AGG	0.513827	1:+85374410	MsG0180004994.01.T01:CDS
TCAATCAACAATGGCACCAA+AGG	0.515027	1:+85374371	None:intergenic
AGCGTGCTTGCAAGTTCACC+AGG	0.516686	1:-85374716	None:intergenic
GAAACCTACCATCACCTCTC+GGG	0.549293	1:+85374665	MsG0180004994.01.T01:CDS
AGAAACCTACCATCACCTCT+CGG	0.550335	1:+85374664	MsG0180004994.01.T01:CDS
CAATCAACAATGGCACCAAA+GGG	0.556792	1:+85374372	None:intergenic
TGCAAGCACGCTGTCTCCGA+GGG	0.572239	1:+85374727	MsG0180004994.01.T02:CDS
TTGCAAGCACGCTGTCTCCG+AGG	0.609490	1:+85374726	MsG0180004994.01.T02:CDS
CATCTTTGAGAAGCTCGCGC+AGG	0.611717	1:+85374615	MsG0180004994.01.T01:CDS
TCCTGACATCGTATCTCACA+AGG	0.651860	1:+85374561	MsG0180004994.01.T01:CDS
TCGTATCTCACAAGGCTATG+GGG	0.663584	1:+85374569	MsG0180004994.01.T01:CDS
ATCTCCCGAGAGGTGATGGT+AGG	0.682571	1:-85374669	None:intergenic
CACCAGGAAGAACAAGACGA+CGG	0.707741	1:-85374700	None:intergenic
GGCAGTCTGAATCTCCCGAG+AGG	0.727018	1:-85374679	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATTCAAGAGCTAGTGAATT+TGG	-	Chr1:85374767-85374786	None:intergenic	30.0%
	CTACAAGATCTACATCTTCA+AGG	+	Chr1:85374522-85374541	MsG0180004994.01.T01:CDS	35.0%
	CTTCTTCTTCTTATCAGTTG+AGG	-	Chr1:85374480-85374499	None:intergenic	35.0%
	CTTGAAGATGTAGATCTTGT+AGG	-	Chr1:85374524-85374543	None:intergenic	35.0%
	ATCGTATCTCACAAGGCTAT+GGG	+	Chr1:85374568-85374587	MsG0180004994.01.T01:CDS	40.0%
!	AGTGAATTTGGTGACAGCTT+TGG	-	Chr1:85374755-85374774	None:intergenic	40.0%
	AGAAACCTACCATCACCTCT+CGG	+	Chr1:85374664-85374683	MsG0180004994.01.T01:CDS	45.0%
	CAGTTGAGGATGCTTCCTTT+GGG	-	Chr1:85374466-85374485	None:intergenic	45.0%
	CATCGTATCTCACAAGGCTA+TGG	+	Chr1:85374567-85374586	MsG0180004994.01.T01:CDS	45.0%
	CATCTTCAAGGTTCTCAAGC+AGG	+	Chr1:85374534-85374553	MsG0180004994.01.T01:CDS	45.0%
	TCAGTTGAGGATGCTTCCTT+TGG	-	Chr1:85374467-85374486	None:intergenic	45.0%
	TCCTGACATCGTATCTCACA+AGG	+	Chr1:85374561-85374580	MsG0180004994.01.T01:CDS	45.0%
	TCGTATCTCACAAGGCTATG+GGG	+	Chr1:85374569-85374588	MsG0180004994.01.T01:CDS	45.0%
!	TCTGCTTTCGTTTTCTCAGC+TGG	-	Chr1:85374438-85374457	None:intergenic	45.0%
	ACGGGTTTCTTCTCTCCCTT+TGG	-	Chr1:85374390-85374409	None:intergenic	50.0%
	AGAGAAGAAGCCTGCAGAGA+AGG	+	Chr1:85374410-85374429	MsG0180004994.01.T01:CDS	50.0%
	CACCAGGAAGAACAAGACGA+CGG	-	Chr1:85374703-85374722	None:intergenic	50.0%
	CGAAAGCAGAGAAGACCCAA+AGG	+	Chr1:85374448-85374467	MsG0180004994.01.T01:intron	50.0%
	CTCTGCAGGCTTCTTCTCTA+CGG	-	Chr1:85374409-85374428	None:intergenic	50.0%
	GAAACCTACCATCACCTCTC+GGG	+	Chr1:85374665-85374684	MsG0180004994.01.T01:CDS	50.0%
	GCCTTGTGAGATACGATGTC+AGG	-	Chr1:85374565-85374584	None:intergenic	50.0%
	TCTGCAGGCTTCTTCTCTAC+GGG	-	Chr1:85374408-85374427	None:intergenic	50.0%
	AGCGTGCTTGCAAGTTCACC+AGG	-	Chr1:85374719-85374738	None:intergenic	55.0%
	CTGAATCTCCCGAGAGGTGA+TGG	-	Chr1:85374676-85374695	None:intergenic	55.0%
!	ATCTCCCGAGAGGTGATGGT+AGG	-	Chr1:85374672-85374691	None:intergenic	55.0%
!	CATCTTTGAGAAGCTCGCGC+AGG	+	Chr1:85374615-85374634	MsG0180004994.01.T01:CDS	55.0%
!	GGTGACAGCTTTGGTTCCCT+CGG	-	Chr1:85374746-85374765	None:intergenic	55.0%
!	TGCCGTCGTCTTGTTCTTCC+TGG	+	Chr1:85374698-85374717	MsG0180004994.01.T01:CDS	55.0%
	GGCAGTCTGAATCTCCCGAG+AGG	-	Chr1:85374682-85374701	None:intergenic	60.0%
	TCAGCTGGTGCCTTCTCTGC+AGG	-	Chr1:85374423-85374442	None:intergenic	60.0%
	TGCAAGCACGCTGTCTCCGA+GGG	+	Chr1:85374727-85374746	MsG0180004994.01.T02:CDS	60.0%
	TTGCAAGCACGCTGTCTCCG+AGG	+	Chr1:85374726-85374745	MsG0180004994.01.T02:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	85374381	85374799	85374381	ID=MsG0180004994.01;Name=MsG0180004994.01
Chr1	mRNA	85374381	85374723	85374381	ID=MsG0180004994.01.T01;Parent=MsG0180004994.01;Name=MsG0180004994.01.T01;_AED=0.28;_eAED=0.28;_QI=0\|0\|0\|1\|0\|0\|2\|0\|99
Chr1	exon	85374381	85374456	85374381	ID=MsG0180004994.01.T01:exon:28223;Parent=MsG0180004994.01.T01
Chr1	exon	85374500	85374723	85374500	ID=MsG0180004994.01.T01:exon:28224;Parent=MsG0180004994.01.T01
Chr1	CDS	85374381	85374456	85374381	ID=MsG0180004994.01.T01:cds;Parent=MsG0180004994.01.T01
Chr1	CDS	85374500	85374723	85374500	ID=MsG0180004994.01.T01:cds;Parent=MsG0180004994.01.T01
Chr1	mRNA	85374611	85374799	85374611	ID=MsG0180004994.01.T02;Parent=MsG0180004994.01;Name=MsG0180004994.01.T02;_AED=0.35;_eAED=0.35;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|62
Chr1	exon	85374611	85374799	85374611	ID=MsG0180004994.01.T02:exon:28225;Parent=MsG0180004994.01.T02
Chr1	CDS	85374611	85374799	85374611	ID=MsG0180004994.01.T02:cds;Parent=MsG0180004994.01.T02
Chr1	mRNA	85374611	85374778	85374611	ID=MsG0180004994.01.T03;Parent=MsG0180004994.01;Name=MsG0180004994.01.T03;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|56
Chr1	exon	85374611	85374778	85374611	ID=MsG0180004994.01.T03:exon:28226;Parent=MsG0180004994.01.T03
Chr1	CDS	85374611	85374778	85374611	ID=MsG0180004994.01.T03:cds;Parent=MsG0180004994.01.T03

Gene Sequence

>MsG0180004994.01.T02

ATGACATCTTTGAGAAGCTCGCGCAGGAATCATCTAGACTCGCTCGCTACAACAGAAACCTACCATCACCTCTCGGGAGATTCAGACTGCCGTCGTCTTGTTCTTCCTGGTGAACTTGCAAGCACGCTGTCTCCGAGGGAACCAAAGCTGTCACCAAATTCACTAGCTCTTGAATTTATACATCTCTAA

>MsG0180004994.01.T03

ATGACATCTTTGAGAAGCTCGCGCAGGAATCATCTAGACTCGCTCGCTACAACAGAAACCTACCATCACCTCTCGGGAGATTCAGACTGCCGTCGTCTTGTTCTTCCTGGTGAACTTGCAAGCACGCTGTCTCCGAGGGAACCAAAGCTGTCACCAAATTCACTAGCT

>MsG0180004994.01.T01

ATGGCACCAAAGGGAGAGAAGAAACCCGTAGAGAAGAAGCCTGCAGAGAAGGCACCAGCTGAGAAAACGAAAGCAGAAAACAAGAAGAGTGTTGAAACCTACAAGATCTACATCTTCAAGGTTCTCAAGCAGGTTCATCCTGACATCGTATCTCACAAGGCTATGGGGATCATGAACAGCTTCATCAATGACATCTTTGAGAAGCTCGCGCAGGAATCATCTAGACTCGCTCGCTACAACAGAAACCTACCATCACCTCTCGGGAGATTCAGACTGCCGTCGTCTTGTTCTTCCTGGTGA

Protein sequence

>MsG0180004994.01.T02

MTSLRSSRRNHLDSLATTETYHHLSGDSDCRRLVLPGELASTLSPREPKLSPNSLALEFIHL*

>MsG0180004994.01.T03

MTSLRSSRRNHLDSLATTETYHHLSGDSDCRRLVLPGELASTLSPREPKLSPNSLA

>MsG0180004994.01.T01

MAPKGEKKPVEKKPAEKAPAEKTKAENKKSVETYKIYIFKVLKQVHPDIVSHKAMGIMNSFINDIFEKLAQESSRLARYNRNLPSPLGRFRLPSSCSSW*