AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021048.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021048.01.T01 MTR_4g064005 96.000 150 4 1 1 150 1 148 7.59e-97 276
MsG0480021048.01.T01 MTR_2g096610 93.893 131 8 0 20 150 18 148 6.31e-84 243
MsG0480021048.01.T01 MTR_4g064967 95.333 150 5 1 1 150 1 148 5.36e-83 241
MsG0480021048.01.T01 MTR_4g063410 94.000 150 7 1 1 150 1 148 4.45e-82 238
MsG0480021048.01.T01 MTR_4g071150 92.763 152 9 1 1 150 1 152 1.16e-81 237
MsG0480021048.01.T01 MTR_4g063280 93.182 132 8 1 20 150 14 145 1.93e-81 236
MsG0480021048.01.T01 MTR_2g096570 87.662 154 14 2 1 150 1 153 1.64e-77 227
MsG0480021048.01.T01 MTR_8g086640 96.212 132 5 0 1 132 1 132 1.24e-74 219
MsG0480021048.01.T01 MTR_7g113160 79.699 133 26 1 10 142 13 144 9.21e-71 209
MsG0480021048.01.T01 MTR_7g114060 74.803 127 28 1 3 129 2 124 7.72e-60 182
MsG0480021048.01.T01 MTR_7g114040 74.803 127 28 1 3 129 2 124 7.72e-60 182
MsG0480021048.01.T01 MTR_7g108320 75.591 127 25 3 4 129 6 127 8.32e-60 182
MsG0480021048.01.T01 MTR_7g093910 73.016 126 31 1 4 129 5 127 3.53e-59 180
MsG0480021048.01.T01 MTR_2g082510 74.790 119 30 0 11 129 3 121 1.17e-58 178
MsG0480021048.01.T01 MTR_2g082220 73.950 119 31 0 11 129 3 121 1.54e-57 176
MsG0480021048.01.T01 MTR_6g005150 64.567 127 31 3 24 149 26 139 4.95e-48 152
MsG0480021048.01.T01 MTR_7g114050 68.333 120 23 3 17 132 10 118 1.82e-45 145
MsG0480021048.01.T01 MTR_4g063300 75.238 105 21 1 45 149 10 109 2.86e-45 144
MsG0480021048.01.T01 MTR_1g011750 59.292 113 44 2 21 132 23 134 6.84e-36 121
MsG0480021048.01.T01 MTR_1g011850 59.292 113 44 2 21 132 23 134 6.84e-36 121
MsG0480021048.01.T01 MTR_1g114050 59.633 109 42 2 21 128 20 127 6.89e-35 118
MsG0480021048.01.T01 MTR_1g071040 61.538 104 38 2 26 128 28 130 3.34e-34 117
MsG0480021048.01.T01 MTR_1g069770 47.917 96 49 1 45 139 9 104 2.31e-24 90.9
MsG0480021048.01.T01 MTR_7g010440 70.588 51 15 0 78 128 122 172 1.58e-16 74.7
MsG0480021048.01.T01 MTR_4g005910 74.419 43 11 0 87 129 4 46 2.83e-16 68.9
MsG0480021048.01.T01 MTR_4g048490 43.810 105 30 2 24 128 26 101 4.00e-16 69.7
MsG0480021048.01.T01 MTR_7g027610 41.818 110 38 3 20 128 10 94 3.89e-15 67.4
MsG0480021048.01.T01 MTR_2g096590 72.500 40 11 0 110 149 12 51 4.92e-12 57.8
MsG0480021048.01.T01 MTR_0653s0030 36.364 99 53 2 56 146 44 140 1.12e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021048.01.T01 AT5G02560 81.046 153 26 1 1 150 1 153 7.31e-78 228
MsG0480021048.01.T01 AT5G27670 76.316 152 32 3 1 150 1 150 4.75e-74 218
MsG0480021048.01.T01 AT5G02560 70.056 177 26 2 1 150 1 177 5.79e-73 216
MsG0480021048.01.T01 AT5G59870 75.000 148 36 1 4 150 3 150 1.34e-71 212
MsG0480021048.01.T01 AT1G51060 71.318 129 37 0 11 139 3 131 7.07e-61 184
MsG0480021048.01.T01 AT4G27230 70.543 129 38 0 11 139 3 131 4.22e-60 182
MsG0480021048.01.T01 AT4G27230 70.543 129 38 0 11 139 3 131 4.22e-60 182
MsG0480021048.01.T01 AT3G20670 71.318 129 37 0 11 139 3 131 7.45e-60 181
MsG0480021048.01.T01 AT5G54640 72.000 125 35 0 11 135 3 127 2.57e-58 177
MsG0480021048.01.T01 AT1G08880 72.222 126 32 1 7 129 2 127 2.89e-58 178
MsG0480021048.01.T01 AT1G54690 73.438 128 28 3 3 129 5 127 1.16e-57 176
MsG0480021048.01.T01 AT2G38810 60.550 109 41 2 21 128 25 132 4.51e-36 121
MsG0480021048.01.T01 AT2G38810 60.550 109 41 2 21 128 25 132 4.51e-36 121
MsG0480021048.01.T01 AT2G38810 60.550 109 41 2 21 128 25 132 4.51e-36 121
MsG0480021048.01.T01 AT2G38810 60.550 109 41 2 21 128 25 132 4.51e-36 121
MsG0480021048.01.T01 AT1G52740 61.905 105 38 2 25 128 27 130 1.03e-34 118
MsG0480021048.01.T01 AT3G54560 57.798 109 44 2 25 132 29 136 7.21e-34 116
MsG0480021048.01.T01 AT3G54560 57.798 109 44 2 25 132 29 136 7.21e-34 116
MsG0480021048.01.T01 AT4G13570 47.826 92 46 2 32 122 28 118 5.44e-20 80.1
MsG0480021048.01.T01 AT4G13570 47.253 91 46 2 33 122 35 124 1.47e-19 79.3

Find 46 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGACTTTCCAGCCTTCTT+TGG 0.239817 4:-52660446 None:intergenic
AATCAGATCTAAGCCTTCTT+TGG 0.251178 4:-52660467 None:intergenic
GAGCTTGGTAAATTACTTTC+TGG 0.258692 4:+52660332 MsG0480021048.01.T01:CDS
CTTGTCACCGACTTCTTCCT+TGG 0.333455 4:-52660086 None:intergenic
CGCTGCTGAAGTTCTTGAAT+TGG 0.356335 4:+52660232 MsG0480021048.01.T01:CDS
CGATTCTTCCCACTGGGAAC+TGG 0.359689 4:-52660127 None:intergenic
TTCTTTGGCTCTTTGTTAGC+AGG 0.375405 4:-52660431 None:intergenic
AGAACTGCTGCTAGGTATAC+TGG 0.389575 4:-52660200 None:intergenic
TTTCATTCTTCTTAGGCAAA+AGG 0.417964 4:-52660400 None:intergenic
TATGCTCAGCGTGTCGGAAC+AGG 0.432163 4:+52660173 MsG0480021048.01.T01:CDS
GGCATGTGTTGTTGGCTGTT+AGG 0.442957 4:+52660300 MsG0480021048.01.T01:CDS
AAAAGGATAGGGTTAATGTT+TGG 0.446113 4:-52660383 None:intergenic
TCCTCCTCCTTTCCTTCCAC+CGG 0.452471 4:-52660063 None:intergenic
GCTGAAGTTCTTGAATTGGC+TGG 0.461152 4:+52660236 MsG0480021048.01.T01:CDS
AACAAAGAGCCAAAGAAGGC+TGG 0.466354 4:+52660437 MsG0480021048.01.T01:CDS
CATCCCAAGGCATGTGTTGT+TGG 0.466727 4:+52660292 MsG0480021048.01.T01:CDS
AAGGGAGCCGGTGGAAGGAA+AGG 0.471425 4:+52660056 MsG0480021048.01.T01:CDS
CAGTTCCCAGTGGGAAGAAT+CGG 0.476269 4:+52660128 MsG0480021048.01.T01:CDS
ACAGCCAACAACACATGCCT+TGG 0.483615 4:-52660296 None:intergenic
TTCTTCTTAGGCAAAAGGAT+AGG 0.488209 4:-52660395 None:intergenic
TCTTCTTAGGCAAAAGGATA+GGG 0.492139 4:-52660394 None:intergenic
TCTGGTGTCACGATTGCTCA+TGG 0.516340 4:+52660350 MsG0480021048.01.T01:CDS
GCCGGTGGAAGGAAAGGAGG+AGG 0.517219 4:+52660062 MsG0480021048.01.T01:CDS
ATCGGTCGATACTTGAAGAA+AGG 0.519021 4:+52660146 MsG0480021048.01.T01:CDS
AGCACGAAGACAACAAAGAA+GGG 0.520726 4:+52660038 MsG0480021048.01.T01:CDS
AAGCACGAAGACAACAAAGA+AGG 0.522115 4:+52660037 MsG0480021048.01.T01:CDS
GGCTGGAAAGTCTCCAAAGA+AGG 0.525629 4:+52660454 MsG0480021048.01.T01:CDS
CAGCCAACAACACATGCCTT+GGG 0.536965 4:-52660295 None:intergenic
CAAAGAAGGGAGCCGGTGGA+AGG 0.542230 4:+52660051 MsG0480021048.01.T01:CDS
TATCGACCGATTCTTCCCAC+TGG 0.544173 4:-52660134 None:intergenic
TGCTAACAAAGAGCCAAAGA+AGG 0.544648 4:+52660433 MsG0480021048.01.T01:CDS
GGAGCCGGTGGAAGGAAAGG+AGG 0.549135 4:+52660059 MsG0480021048.01.T01:CDS
GTTAGGAACGATGAAGAGCT+TGG 0.552797 4:+52660317 MsG0480021048.01.T01:CDS
GAAGGAAAGGAGGAGGACCA+AGG 0.555100 4:+52660069 MsG0480021048.01.T01:CDS
TCCCACTGGGAACTGGAGAC+CGG 0.556836 4:-52660120 None:intergenic
GGAAGATATGCTCAGCGTGT+CGG 0.591142 4:+52660167 MsG0480021048.01.T01:CDS
GATATTCAAGAACTGCTGCT+AGG 0.592671 4:-52660208 None:intergenic
ACAACAAAGAAGGGAGCCGG+TGG 0.595449 4:+52660047 MsG0480021048.01.T01:CDS
AAGACAACAAAGAAGGGAGC+CGG 0.597879 4:+52660044 MsG0480021048.01.T01:CDS
GTGACAAGATCAATCAGAGC+CGG 0.601599 4:+52660101 MsG0480021048.01.T01:CDS
AGGAGGACCAAGGAAGAAGT+CGG 0.610829 4:+52660079 MsG0480021048.01.T01:CDS
GCCGGTCTCCAGTTCCCAGT+GGG 0.615696 4:+52660119 MsG0480021048.01.T01:CDS
ATCGACCGATTCTTCCCACT+GGG 0.626884 4:-52660133 None:intergenic
AGAAGAATAGAATCATCCCA+AGG 0.679264 4:+52660279 MsG0480021048.01.T01:CDS
AGCCGGTCTCCAGTTCCCAG+TGG 0.691656 4:+52660118 MsG0480021048.01.T01:CDS
GGTGTCACGATTGCTCATGG+TGG 0.707991 4:+52660353 MsG0480021048.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAAGGATAGGGTTAATGTT+TGG - Chr4:52660386-52660405 None:intergenic 30.0%
TTTCATTCTTCTTAGGCAAA+AGG - Chr4:52660403-52660422 None:intergenic 30.0%
AGAAGAATAGAATCATCCCA+AGG + Chr4:52660279-52660298 MsG0480021048.01.T01:CDS 35.0%
GAGCTTGGTAAATTACTTTC+TGG + Chr4:52660332-52660351 MsG0480021048.01.T01:CDS 35.0%
TCTTCTTAGGCAAAAGGATA+GGG - Chr4:52660397-52660416 None:intergenic 35.0%
TTCTTCTTAGGCAAAAGGAT+AGG - Chr4:52660398-52660417 None:intergenic 35.0%
!!! GCAGCTTTTTCATTCTTCTT+AGG - Chr4:52660410-52660429 None:intergenic 35.0%
AAGCACGAAGACAACAAAGA+AGG + Chr4:52660037-52660056 MsG0480021048.01.T01:CDS 40.0%
AGCACGAAGACAACAAAGAA+GGG + Chr4:52660038-52660057 MsG0480021048.01.T01:CDS 40.0%
ATCGGTCGATACTTGAAGAA+AGG + Chr4:52660146-52660165 MsG0480021048.01.T01:CDS 40.0%
GATATTCAAGAACTGCTGCT+AGG - Chr4:52660211-52660230 None:intergenic 40.0%
TGCTAACAAAGAGCCAAAGA+AGG + Chr4:52660433-52660452 MsG0480021048.01.T01:CDS 40.0%
!! TTCTTTGGCTCTTTGTTAGC+AGG - Chr4:52660434-52660453 None:intergenic 40.0%
AACAAAGAGCCAAAGAAGGC+TGG + Chr4:52660437-52660456 MsG0480021048.01.T01:CDS 45.0%
AAGACAACAAAGAAGGGAGC+CGG + Chr4:52660044-52660063 MsG0480021048.01.T01:CDS 45.0%
AGAACTGCTGCTAGGTATAC+TGG - Chr4:52660203-52660222 None:intergenic 45.0%
CGCTGCTGAAGTTCTTGAAT+TGG + Chr4:52660232-52660251 MsG0480021048.01.T01:CDS 45.0%
GCTGAAGTTCTTGAATTGGC+TGG + Chr4:52660236-52660255 MsG0480021048.01.T01:CDS 45.0%
GTGACAAGATCAATCAGAGC+CGG + Chr4:52660101-52660120 MsG0480021048.01.T01:CDS 45.0%
GTTAGGAACGATGAAGAGCT+TGG + Chr4:52660317-52660336 MsG0480021048.01.T01:CDS 45.0%
ACAGCCAACAACACATGCCT+TGG - Chr4:52660299-52660318 None:intergenic 50.0%
AGGAGGACCAAGGAAGAAGT+CGG + Chr4:52660079-52660098 MsG0480021048.01.T01:CDS 50.0%
CAGCCAACAACACATGCCTT+GGG - Chr4:52660298-52660317 None:intergenic 50.0%
CAGTTCCCAGTGGGAAGAAT+CGG + Chr4:52660128-52660147 MsG0480021048.01.T01:CDS 50.0%
CATCCCAAGGCATGTGTTGT+TGG + Chr4:52660292-52660311 MsG0480021048.01.T01:CDS 50.0%
GGAAGATATGCTCAGCGTGT+CGG + Chr4:52660167-52660186 MsG0480021048.01.T01:CDS 50.0%
GGAGACTTTCCAGCCTTCTT+TGG - Chr4:52660449-52660468 None:intergenic 50.0%
GGCTGGAAAGTCTCCAAAGA+AGG + Chr4:52660454-52660473 MsG0480021048.01.T01:CDS 50.0%
! ATCGACCGATTCTTCCCACT+GGG - Chr4:52660136-52660155 None:intergenic 50.0%
! GGCATGTGTTGTTGGCTGTT+AGG + Chr4:52660300-52660319 MsG0480021048.01.T01:CDS 50.0%
! TATCGACCGATTCTTCCCAC+TGG - Chr4:52660137-52660156 None:intergenic 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG - Chr4:52660089-52660108 None:intergenic 50.0%
!! TCTGGTGTCACGATTGCTCA+TGG + Chr4:52660350-52660369 MsG0480021048.01.T01:CDS 50.0%
ACAACAAAGAAGGGAGCCGG+TGG + Chr4:52660047-52660066 MsG0480021048.01.T01:CDS 55.0%
CGATTCTTCCCACTGGGAAC+TGG - Chr4:52660130-52660149 None:intergenic 55.0%
GAAGGAAAGGAGGAGGACCA+AGG + Chr4:52660069-52660088 MsG0480021048.01.T01:CDS 55.0%
TATGCTCAGCGTGTCGGAAC+AGG + Chr4:52660173-52660192 MsG0480021048.01.T01:CDS 55.0%
TCCTCCTCCTTTCCTTCCAC+CGG - Chr4:52660066-52660085 None:intergenic 55.0%
! GGTGTCACGATTGCTCATGG+TGG + Chr4:52660353-52660372 MsG0480021048.01.T01:CDS 55.0%
AAGGGAGCCGGTGGAAGGAA+AGG + Chr4:52660056-52660075 MsG0480021048.01.T01:CDS 60.0%
CAAAGAAGGGAGCCGGTGGA+AGG + Chr4:52660051-52660070 MsG0480021048.01.T01:CDS 60.0%
TCCCACTGGGAACTGGAGAC+CGG - Chr4:52660123-52660142 None:intergenic 60.0%
AGCCGGTCTCCAGTTCCCAG+TGG + Chr4:52660118-52660137 MsG0480021048.01.T01:CDS 65.0%
GCCGGTCTCCAGTTCCCAGT+GGG + Chr4:52660119-52660138 MsG0480021048.01.T01:CDS 65.0%
GCCGGTGGAAGGAAAGGAGG+AGG + Chr4:52660062-52660081 MsG0480021048.01.T01:CDS 65.0%
GGAGCCGGTGGAAGGAAAGG+AGG + Chr4:52660059-52660078 MsG0480021048.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 52660029 52660481 52660029 ID=MsG0480021048.01;Name=MsG0480021048.01
Chr4 mRNA 52660029 52660481 52660029 ID=MsG0480021048.01.T01;Parent=MsG0480021048.01;Name=MsG0480021048.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|150
Chr4 exon 52660029 52660481 52660029 ID=MsG0480021048.01.T01:exon:19968;Parent=MsG0480021048.01.T01
Chr4 CDS 52660029 52660481 52660029 ID=MsG0480021048.01.T01:cds;Parent=MsG0480021048.01.T01
Gene Sequence

>MsG0480021048.01.T01

ATGGAGGCAAGCACGAAGACAACAAAGAAGGGAGCCGGTGGAAGGAAAGGAGGAGGACCAAGGAAGAAGTCGGTGACAAGATCAATCAGAGCCGGTCTCCAGTTCCCAGTGGGAAGAATCGGTCGATACTTGAAGAAAGGAAGATATGCTCAGCGTGTCGGAACAGGTGCACCAGTATACCTAGCAGCAGTTCTTGAATATCTCGCTGCTGAAGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATAGAATCATCCCAAGGCATGTGTTGTTGGCTGTTAGGAACGATGAAGAGCTTGGTAAATTACTTTCTGGTGTCACGATTGCTCATGGTGGCGTTCTTCCAAACATTAACCCTATCCTTTTGCCTAAGAAGAATGAAAAAGCTGCTCCTGCTAACAAAGAGCCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MsG0480021048.01.T01

MEASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLSGVTIAHGGVLPNINPILLPKKNEKAAPANKEPKKAGKSPKKA*