AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017932.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017932.01.T01 MTR_3g115300 97.159 176 5 0 1 176 1 176 5.30e-124 348
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017932.01.T01 AT5G42330 51.240 121 51 5 6 121 2 119 6.30e-27 101

Find 59 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACAATTGCTTCTGAATTT+AGG 0.081710 3:+102315940 MsG0380017932.01.T01:CDS
CACAATTGCTTCTGAATTTA+GGG 0.195473 3:+102315941 MsG0380017932.01.T01:CDS
TGAAACAGAACACCAGATTT+AGG 0.204443 3:-102316038 None:intergenic
GAAGCAATTGTGCGGTATTC+AGG 0.265276 3:-102315930 None:intergenic
AGGATGGTTCGTGACGAATT+TGG 0.279654 3:+102315972 MsG0380017932.01.T01:CDS
TCGGTCGCGGTGGTCGGAGG+TGG 0.289193 3:-102315682 None:intergenic
TGGTCGGAGGTGGGATTTCA+TGG 0.291163 3:-102315672 None:intergenic
CTTGATTTAATATGATTGTA+TGG 0.314919 3:+102316448 MsG0380017932.01.T01:three_prime_UTR
GAAACAGAACACCAGATTTA+GGG 0.318085 3:-102316037 None:intergenic
CTTGTGTTTACTGTTGAATC+TGG 0.325864 3:+102316191 MsG0380017932.01.T01:CDS
CCAGATCCGGAAGAAATTAA+TGG 0.326179 3:+102315999 MsG0380017932.01.T01:CDS
CCATTAATTTCTTCCGGATC+TGG 0.333300 3:-102315999 None:intergenic
TTTAACCAAGTCTCGGAAAC+TGG 0.342643 3:+102315887 MsG0380017932.01.T01:CDS
AGATTTAGGGTTACAAGAAT+TGG 0.342948 3:-102316024 None:intergenic
TTGGTACCATTAATTTCTTC+CGG 0.348296 3:-102316005 None:intergenic
CGAAGACGCATCGGAGACTT+AGG 0.358488 3:-102315707 None:intergenic
CGGTATTCAGGTCGTAGTTC+AGG 0.361330 3:-102315918 None:intergenic
AATTCTTGTAACCCTAAATC+TGG 0.396987 3:+102316026 MsG0380017932.01.T01:CDS
AAAATGTCTCAATTTCCATT+TGG 0.410440 3:-102316511 None:intergenic
AAGAATACTGCAAGAAAACT+TGG 0.439698 3:+102316218 MsG0380017932.01.T01:CDS
CCCTCTCTTCAAACTCCACC+AGG 0.453304 3:+102315752 MsG0380017932.01.T01:CDS
GAGAGGTTGAAGAGAAAGAA+AGG 0.462625 3:+102316101 MsG0380017932.01.T01:CDS
TAGGGATCGGTCGCGGTGGT+CGG 0.470489 3:-102315688 None:intergenic
TTGAACCAGTTTCCGAGACT+TGG 0.470567 3:-102315892 None:intergenic
TTATGATGATTATTCACCTT+AGG 0.472768 3:-102316164 None:intergenic
CGGTCGCGGTGGTCGGAGGT+GGG 0.481693 3:-102315681 None:intergenic
CTGAATTTAGGGCTTTATCA+AGG 0.492327 3:+102315952 MsG0380017932.01.T01:CDS
CGAATTTGGAAAACCAGATC+CGG 0.501998 3:+102315986 MsG0380017932.01.T01:CDS
AATAAGAAACCTCCTCTAGC+TGG 0.510440 3:+102316324 MsG0380017932.01.T01:three_prime_UTR
GTTGGAGCATCGAGAAGAGT+AGG 0.523400 3:+102316386 MsG0380017932.01.T01:three_prime_UTR
ACTGTTGGTGAGAAGAAAGT+TGG 0.529021 3:+102316368 MsG0380017932.01.T01:three_prime_UTR
GGATCGGTCGCGGTGGTCGG+AGG 0.529657 3:-102315685 None:intergenic
ATTTAGGGCTTTATCAAGGA+TGG 0.529903 3:+102315956 MsG0380017932.01.T01:CDS
CCTGGTGGAGTTTGAAGAGA+GGG 0.535099 3:-102315752 None:intergenic
CAGGTTCTTTAACCAAGTCT+CGG 0.545491 3:+102315880 MsG0380017932.01.T01:intron
TTCTGCTGCTTATTCTGCTG+AGG 0.545660 3:+102316259 MsG0380017932.01.T01:CDS
TGTGACTGAAAATGCGGTGC+GGG 0.546036 3:-102315620 None:intergenic
ACCTGGTGGAGTTTGAAGAG+AGG 0.555239 3:-102315753 None:intergenic
GAAGACGCATCGGAGACTTA+GGG 0.556071 3:-102315706 None:intergenic
TTGATTTAATATGATTGTAT+GGG 0.562573 3:+102316449 MsG0380017932.01.T01:three_prime_UTR
TACTAGTATTAAGCAACCTA+AGG 0.572843 3:+102316148 MsG0380017932.01.T01:CDS
ATGATGAGTATTCTGCAAGA+AGG 0.596236 3:+102316075 MsG0380017932.01.T01:CDS
ATTTGATAAACTGTTACTGT+TGG 0.597164 3:+102316353 MsG0380017932.01.T01:three_prime_UTR
ATGCTTCTAAGCATATACCT+TGG 0.602130 3:-102316292 None:intergenic
TCAAATCTTGAACCAGCTAG+AGG 0.605228 3:-102316336 None:intergenic
CGCATCGGAGACTTAGGGAT+CGG 0.618390 3:-102315701 None:intergenic
AAGAGAGGGAGAGTCAAGAA+CGG 0.623596 3:-102315738 None:intergenic
GTTCGAGTTCGAAGACGCAT+CGG 0.624113 3:-102315716 None:intergenic
GGAGACTTAGGGATCGGTCG+CGG 0.628168 3:-102315695 None:intergenic
AATCTTGAACCAGCTAGAGG+AGG 0.634666 3:-102316333 None:intergenic
GACTTAGGGATCGGTCGCGG+TGG 0.645567 3:-102315692 None:intergenic
GAAGAGAAAGAAAGGAGTCA+CGG 0.667345 3:+102316109 MsG0380017932.01.T01:CDS
TCTGGTGTTCTGTTTCAGAG+AGG 0.698623 3:+102316044 MsG0380017932.01.T01:CDS
GCTGAGGTTAGTGAACTCCA+AGG 0.703163 3:+102316275 MsG0380017932.01.T01:CDS
ATTCTGCAAGAAGGAACGAG+AGG 0.711042 3:+102316084 MsG0380017932.01.T01:CDS
GCAATGTGTGACTGAAAATG+CGG 0.711864 3:-102315626 None:intergenic
TAAATTCAGAAGCAATTGTG+CGG 0.713706 3:-102315938 None:intergenic
GAGAAAGAAAGGAGTCACGG+TGG 0.729465 3:+102316112 MsG0380017932.01.T01:CDS
GTGTGACTGAAAATGCGGTG+CGG 0.745677 3:-102315621 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATTTAATATGATTGTAT+GGG + Chr3:102316449-102316468 MsG0380017932.01.T01:three_prime_UTR 15.0%
!! CTTGATTTAATATGATTGTA+TGG + Chr3:102316448-102316467 MsG0380017932.01.T01:three_prime_UTR 20.0%
! AAAATGTCTCAATTTCCATT+TGG - Chr3:102316514-102316533 None:intergenic 25.0%
! ATTTGATAAACTGTTACTGT+TGG + Chr3:102316353-102316372 MsG0380017932.01.T01:three_prime_UTR 25.0%
! GAAATAAGATTATAAGAACC+TGG - Chr3:102315773-102315792 None:intergenic 25.0%
! TAGGAAAAATTCGTACTATT+GGG + Chr3:102316405-102316424 MsG0380017932.01.T01:three_prime_UTR 25.0%
! TTATGATGATTATTCACCTT+AGG - Chr3:102316167-102316186 None:intergenic 25.0%
!!! TTCTGTTACTTTTTACCAAA+TGG + Chr3:102316496-102316515 MsG0380017932.01.T01:three_prime_UTR 25.0%
AAGAATACTGCAAGAAAACT+TGG + Chr3:102316218-102316237 MsG0380017932.01.T01:CDS 30.0%
AATTCTTGTAACCCTAAATC+TGG + Chr3:102316026-102316045 MsG0380017932.01.T01:CDS 30.0%
ATAAGATTATAAGAACCTGG+TGG - Chr3:102315770-102315789 None:intergenic 30.0%
CACAATTGCTTCTGAATTTA+GGG + Chr3:102315941-102315960 MsG0380017932.01.T01:CDS 30.0%
GTAGGAAAAATTCGTACTAT+TGG + Chr3:102316404-102316423 MsG0380017932.01.T01:three_prime_UTR 30.0%
GTGGATTTGAAAATTGAAAC+AGG + Chr3:102315861-102315880 MsG0380017932.01.T01:intron 30.0%
TAAATTCAGAAGCAATTGTG+CGG - Chr3:102315941-102315960 None:intergenic 30.0%
TACTAGTATTAAGCAACCTA+AGG + Chr3:102316148-102316167 MsG0380017932.01.T01:CDS 30.0%
TCCACAAACAAAAAATGTCA+TGG - Chr3:102315846-102315865 None:intergenic 30.0%
TTGGTACCATTAATTTCTTC+CGG - Chr3:102316008-102316027 None:intergenic 30.0%
! AGATTTAGGGTTACAAGAAT+TGG - Chr3:102316027-102316046 None:intergenic 30.0%
!!! TCCATGACATTTTTTGTTTG+TGG + Chr3:102315842-102315861 MsG0380017932.01.T01:intron 30.0%
ATGATGAGTATTCTGCAAGA+AGG + Chr3:102316075-102316094 MsG0380017932.01.T01:CDS 35.0%
ATGCTTCTAAGCATATACCT+TGG - Chr3:102316295-102316314 None:intergenic 35.0%
CTTGTGTTTACTGTTGAATC+TGG + Chr3:102316191-102316210 MsG0380017932.01.T01:CDS 35.0%
GAAACAGAACACCAGATTTA+GGG - Chr3:102316040-102316059 None:intergenic 35.0%
GCACAATTGCTTCTGAATTT+AGG + Chr3:102315940-102315959 MsG0380017932.01.T01:CDS 35.0%
TGAAACAGAACACCAGATTT+AGG - Chr3:102316041-102316060 None:intergenic 35.0%
! ATTTAGGGCTTTATCAAGGA+TGG + Chr3:102315956-102315975 MsG0380017932.01.T01:CDS 35.0%
! CTGAATTTAGGGCTTTATCA+AGG + Chr3:102315952-102315971 MsG0380017932.01.T01:CDS 35.0%
!! TGCGGGTTTTTATTTGATTG+TGG - Chr3:102315606-102315625 None:intergenic 35.0%
AATAAGAAACCTCCTCTAGC+TGG + Chr3:102316324-102316343 MsG0380017932.01.T01:three_prime_UTR 40.0%
CAGGTTCTTTAACCAAGTCT+CGG + Chr3:102315880-102315899 MsG0380017932.01.T01:intron 40.0%
CCAGATCCGGAAGAAATTAA+TGG + Chr3:102315999-102316018 MsG0380017932.01.T01:CDS 40.0%
CCATTAATTTCTTCCGGATC+TGG - Chr3:102316002-102316021 None:intergenic 40.0%
CGAATTTGGAAAACCAGATC+CGG + Chr3:102315986-102316005 MsG0380017932.01.T01:CDS 40.0%
GAAGAGAAAGAAAGGAGTCA+CGG + Chr3:102316109-102316128 MsG0380017932.01.T01:CDS 40.0%
GAGAGGTTGAAGAGAAAGAA+AGG + Chr3:102316101-102316120 MsG0380017932.01.T01:CDS 40.0%
GCAATGTGTGACTGAAAATG+CGG - Chr3:102315629-102315648 None:intergenic 40.0%
TCAAATCTTGAACCAGCTAG+AGG - Chr3:102316339-102316358 None:intergenic 40.0%
TTTAACCAAGTCTCGGAAAC+TGG + Chr3:102315887-102315906 MsG0380017932.01.T01:CDS 40.0%
! ACTGTTGGTGAGAAGAAAGT+TGG + Chr3:102316368-102316387 MsG0380017932.01.T01:three_prime_UTR 40.0%
AAGAGAGGGAGAGTCAAGAA+CGG - Chr3:102315741-102315760 None:intergenic 45.0%
AATCTTGAACCAGCTAGAGG+AGG - Chr3:102316336-102316355 None:intergenic 45.0%
AGGATGGTTCGTGACGAATT+TGG + Chr3:102315972-102315991 MsG0380017932.01.T01:CDS 45.0%
ATTCTGCAAGAAGGAACGAG+AGG + Chr3:102316084-102316103 MsG0380017932.01.T01:CDS 45.0%
GAAGCAATTGTGCGGTATTC+AGG - Chr3:102315933-102315952 None:intergenic 45.0%
TCTGGTGTTCTGTTTCAGAG+AGG + Chr3:102316044-102316063 MsG0380017932.01.T01:CDS 45.0%
TTGAACCAGTTTCCGAGACT+TGG - Chr3:102315895-102315914 None:intergenic 45.0%
! TTCTGCTGCTTATTCTGCTG+AGG + Chr3:102316259-102316278 MsG0380017932.01.T01:CDS 45.0%
ACCTGGTGGAGTTTGAAGAG+AGG - Chr3:102315756-102315775 None:intergenic 50.0%
CCTGGTGGAGTTTGAAGAGA+GGG - Chr3:102315755-102315774 None:intergenic 50.0%
CGGTATTCAGGTCGTAGTTC+AGG - Chr3:102315921-102315940 None:intergenic 50.0%
GAAGACGCATCGGAGACTTA+GGG - Chr3:102315709-102315728 None:intergenic 50.0%
GAGAAAGAAAGGAGTCACGG+TGG + Chr3:102316112-102316131 MsG0380017932.01.T01:CDS 50.0%
GCTGAGGTTAGTGAACTCCA+AGG + Chr3:102316275-102316294 MsG0380017932.01.T01:CDS 50.0%
GTGTGACTGAAAATGCGGTG+CGG - Chr3:102315624-102315643 None:intergenic 50.0%
TGTGACTGAAAATGCGGTGC+GGG - Chr3:102315623-102315642 None:intergenic 50.0%
! GTTCGAGTTCGAAGACGCAT+CGG - Chr3:102315719-102315738 None:intergenic 50.0%
!! GTTGGAGCATCGAGAAGAGT+AGG + Chr3:102316386-102316405 MsG0380017932.01.T01:three_prime_UTR 50.0%
CCCTCTCTTCAAACTCCACC+AGG + Chr3:102315752-102315771 MsG0380017932.01.T01:CDS 55.0%
CGAAGACGCATCGGAGACTT+AGG - Chr3:102315710-102315729 None:intergenic 55.0%
CGCATCGGAGACTTAGGGAT+CGG - Chr3:102315704-102315723 None:intergenic 55.0%
TGGTCGGAGGTGGGATTTCA+TGG - Chr3:102315675-102315694 None:intergenic 55.0%
GGAGACTTAGGGATCGGTCG+CGG - Chr3:102315698-102315717 None:intergenic 60.0%
GACTTAGGGATCGGTCGCGG+TGG - Chr3:102315695-102315714 None:intergenic 65.0%
TAGGGATCGGTCGCGGTGGT+CGG - Chr3:102315691-102315710 None:intergenic 65.0%
CGGTCGCGGTGGTCGGAGGT+GGG - Chr3:102315684-102315703 None:intergenic 75.0%
GGATCGGTCGCGGTGGTCGG+AGG - Chr3:102315688-102315707 None:intergenic 75.0%
TCGGTCGCGGTGGTCGGAGG+TGG - Chr3:102315685-102315704 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 102315595 102316541 102315595 ID=MsG0380017932.01;Name=MsG0380017932.01
Chr3 mRNA 102315595 102316541 102315595 ID=MsG0380017932.01.T01;Parent=MsG0380017932.01;Name=MsG0380017932.01.T01;_AED=0.37;_eAED=0.37;_QI=58|1|1|1|1|1|2|225|184
Chr3 exon 102315595 102315773 102315595 ID=MsG0380017932.01.T01:exon:13291;Parent=MsG0380017932.01.T01
Chr3 exon 102315883 102316541 102315883 ID=MsG0380017932.01.T01:exon:13292;Parent=MsG0380017932.01.T01
Chr3 five_prime_UTR 102315595 102315652 102315595 ID=MsG0380017932.01.T01:five_prime_utr;Parent=MsG0380017932.01.T01
Chr3 CDS 102315653 102315773 102315653 ID=MsG0380017932.01.T01:cds;Parent=MsG0380017932.01.T01
Chr3 CDS 102315883 102316316 102315883 ID=MsG0380017932.01.T01:cds;Parent=MsG0380017932.01.T01
Chr3 three_prime_UTR 102316317 102316541 102316317 ID=MsG0380017932.01.T01:three_prime_utr;Parent=MsG0380017932.01.T01
Gene Sequence

>MsG0380017932.01.T01

ATGTACTATTCATCATCAACCATGAAATCCCACCTCCGACCACCGCGACCGATCCCTAAGTCTCCGATGCGTCTTCGAACTCGAACCGTTCTTGACTCTCCCTCTCTTCAAACTCCACCAGGTTCTTTAACCAAGTCTCGGAAACTGGTTCAATCTCCTGAACTACGACCTGAATACCGCACAATTGCTTCTGAATTTAGGGCTTTATCAAGGATGGTTCGTGACGAATTTGGAAAACCAGATCCGGAAGAAATTAATGGTACCAATTCTTGTAACCCTAAATCTGGTGTTCTGTTTCAGAGAGGAAGATTCTATGATGAGTATTCTGCAAGAAGGAACGAGAGGTTGAAGAGAAAGAAAGGAGTCACGGTGGATGAAGTTAATACTACTAGTATTAAGCAACCTAAGGTGAATAATCATCATAATGTTCTTGTGTTTACTGTTGAATCTGGAAAAAAGAATACTGCAAGAAAACTTGGAAGTTTGAGAAAATCTGTTTCTGCTGCTTATTCTGCTGAGGTTAGTGAACTCCAAGGTATATGCTTAGAAGCATGA

Protein sequence

>MsG0380017932.01.T01

MYYSSSTMKSHLRPPRPIPKSPMRLRTRTVLDSPSLQTPPGSLTKSRKLVQSPELRPEYRTIASEFRALSRMVRDEFGKPDPEEINGTNSCNPKSGVLFQRGRFYDEYSARRNERLKRKKGVTVDEVNTTSIKQPKVNNHHNVLVFTVESGKKNTARKLGSLRKSVSAAYSAEVSELQGICLEA*