AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020921.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020921.01.T01 MTR_4g064005 95.333 150 5 1 1 150 1 148 2.38e-96 274
MsG0480020921.01.T01 MTR_2g096610 88.667 150 15 1 1 150 1 148 2.90e-84 244
MsG0480020921.01.T01 MTR_4g064967 96.000 150 4 1 1 150 1 148 9.06e-84 243
MsG0480020921.01.T01 MTR_4g071150 93.421 152 8 1 1 150 1 152 2.16e-82 239
MsG0480020921.01.T01 MTR_4g063410 93.333 150 8 1 1 150 1 148 1.60e-81 237
MsG0480020921.01.T01 MTR_4g063280 93.182 132 8 1 20 150 14 145 1.99e-81 236
MsG0480020921.01.T01 MTR_2g096570 88.312 154 13 2 1 150 1 153 3.24e-78 229
MsG0480020921.01.T01 MTR_8g086640 96.970 132 4 0 1 132 1 132 1.81e-75 222
MsG0480020921.01.T01 MTR_7g113160 79.699 133 26 1 10 142 13 144 1.09e-70 209
MsG0480020921.01.T01 MTR_7g108320 75.591 127 25 3 4 129 6 127 7.62e-60 182
MsG0480020921.01.T01 MTR_7g114060 74.803 127 28 1 3 129 2 124 8.16e-60 182
MsG0480020921.01.T01 MTR_7g114040 74.803 127 28 1 3 129 2 124 8.16e-60 182
MsG0480020921.01.T01 MTR_7g093910 73.016 126 31 1 4 129 5 127 4.35e-59 180
MsG0480020921.01.T01 MTR_2g082510 74.790 119 30 0 11 129 3 121 1.35e-58 178
MsG0480020921.01.T01 MTR_2g082220 73.950 119 31 0 11 129 3 121 1.63e-57 176
MsG0480020921.01.T01 MTR_6g005150 64.567 127 31 3 24 149 26 139 4.95e-48 152
MsG0480020921.01.T01 MTR_7g114050 68.333 120 23 3 17 132 10 118 2.22e-45 145
MsG0480020921.01.T01 MTR_4g063300 75.238 105 21 1 45 149 10 109 3.12e-45 144
MsG0480020921.01.T01 MTR_1g011750 59.292 113 44 2 21 132 23 134 7.07e-36 121
MsG0480020921.01.T01 MTR_1g011850 59.292 113 44 2 21 132 23 134 7.07e-36 121
MsG0480020921.01.T01 MTR_1g114050 59.633 109 42 2 21 128 20 127 7.35e-35 118
MsG0480020921.01.T01 MTR_1g071040 61.538 104 38 2 26 128 28 130 3.53e-34 116
MsG0480020921.01.T01 MTR_1g069770 47.917 96 49 1 45 139 9 104 2.44e-24 90.9
MsG0480020921.01.T01 MTR_7g010440 70.588 51 15 0 78 128 122 172 1.79e-16 74.7
MsG0480020921.01.T01 MTR_4g005910 74.419 43 11 0 87 129 4 46 2.80e-16 68.9
MsG0480020921.01.T01 MTR_4g048490 43.810 105 30 2 24 128 26 101 3.88e-16 69.7
MsG0480020921.01.T01 MTR_7g027610 41.818 110 38 3 20 128 10 94 4.06e-15 67.4
MsG0480020921.01.T01 MTR_2g096590 72.500 40 11 0 110 149 12 51 4.66e-12 57.8
MsG0480020921.01.T01 MTR_0653s0030 36.364 99 53 2 56 146 44 140 1.08e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020921.01.T01 AT5G02560 81.699 153 25 1 1 150 1 153 1.25e-78 230
MsG0480020921.01.T01 AT5G02560 70.621 177 25 2 1 150 1 177 1.11e-73 218
MsG0480020921.01.T01 AT5G27670 75.658 152 33 3 1 150 1 150 1.77e-73 217
MsG0480020921.01.T01 AT5G59870 75.000 148 36 1 4 150 3 150 1.39e-71 212
MsG0480020921.01.T01 AT1G51060 71.318 129 37 0 11 139 3 131 9.72e-61 184
MsG0480020921.01.T01 AT4G27230 70.543 129 38 0 11 139 3 131 4.31e-60 182
MsG0480020921.01.T01 AT4G27230 70.543 129 38 0 11 139 3 131 4.31e-60 182
MsG0480020921.01.T01 AT3G20670 71.318 129 37 0 11 139 3 131 9.18e-60 181
MsG0480020921.01.T01 AT1G08880 72.222 126 32 1 7 129 2 127 2.96e-58 178
MsG0480020921.01.T01 AT5G54640 72.000 125 35 0 11 135 3 127 3.06e-58 177
MsG0480020921.01.T01 AT1G54690 73.438 128 28 3 3 129 5 127 1.30e-57 176
MsG0480020921.01.T01 AT2G38810 60.550 109 41 2 21 128 25 132 5.08e-36 121
MsG0480020921.01.T01 AT2G38810 60.550 109 41 2 21 128 25 132 5.08e-36 121
MsG0480020921.01.T01 AT2G38810 60.550 109 41 2 21 128 25 132 5.08e-36 121
MsG0480020921.01.T01 AT2G38810 60.550 109 41 2 21 128 25 132 5.08e-36 121
MsG0480020921.01.T01 AT1G52740 61.905 105 38 2 25 128 27 130 1.07e-34 118
MsG0480020921.01.T01 AT3G54560 57.798 109 44 2 25 132 29 136 7.05e-34 116
MsG0480020921.01.T01 AT3G54560 57.798 109 44 2 25 132 29 136 7.05e-34 116
MsG0480020921.01.T01 AT4G13570 47.826 92 46 2 32 122 28 118 5.50e-20 80.1
MsG0480020921.01.T01 AT4G13570 47.253 91 46 2 33 122 35 124 1.47e-19 79.3

Find 45 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGACTTTCCAGCCTTCTT+TGG 0.239817 4:+50606314 None:intergenic
GATCAGATCTAAGCCTTCTT+TGG 0.251178 4:+50606293 None:intergenic
GAGCTTGGTAAATTACTTTC+TGG 0.258692 4:-50606428 MsG0480020921.01.T01:CDS
GAGCGTATCTTCCTTTCTTC+AGG 0.308141 4:+50606603 None:intergenic
TTCTTTGGCTCTTTGTTAGC+TGG 0.326267 4:+50606329 None:intergenic
CTTGTCACCGACTTCTTCCT+TGG 0.335895 4:+50606674 None:intergenic
GGAGCCGGAGGAAGGAAGGG+TGG 0.384609 4:-50606701 MsG0480020921.01.T01:CDS
AGAACTGCTGCTAGGTATAC+TGG 0.389575 4:+50606560 None:intergenic
CAATTTCCAGTTGGAAGAAT+CGG 0.397925 4:-50606632 MsG0480020921.01.T01:CDS
TACGCTCAGCGTGTTGGAAC+AGG 0.407073 4:-50606587 MsG0480020921.01.T01:CDS
GCCGGAGGAAGGAAGGGTGG+AGG 0.421195 4:-50606698 MsG0480020921.01.T01:CDS
GAAGGGAGCCGGAGGAAGGA+AGG 0.422190 4:-50606705 MsG0480020921.01.T01:CDS
AAGAGGATAGGGTTAATGTT+TGG 0.423690 4:+50606377 None:intergenic
TGCTGCTGAAGTTCTTGAGT+TGG 0.438702 4:-50606528 MsG0480020921.01.T01:CDS
GGCATGTGTTGTTGGCTGTT+AGG 0.442957 4:-50606460 MsG0480020921.01.T01:CDS
AACAAAGAGCCAAAGAAGGC+TGG 0.466354 4:-50606323 MsG0480020921.01.T01:CDS
TCCTCCACCCTTCCTTCCTC+CGG 0.466462 4:+50606697 None:intergenic
TATTCCAAGGCATGTGTTGT+TGG 0.470522 4:-50606468 MsG0480020921.01.T01:CDS
GCTGAAGTTCTTGAGTTGGC+TGG 0.471187 4:-50606524 MsG0480020921.01.T01:CDS
TCTTCTTAGGCAAGAGGATA+GGG 0.474984 4:+50606366 None:intergenic
TATCGACCGATTCTTCCAAC+TGG 0.484363 4:+50606626 None:intergenic
AAATTGAAGACCGGCTCTGA+TGG 0.488889 4:+50606649 None:intergenic
TTCTTCTTAGGCAAGAGGAT+AGG 0.503964 4:+50606365 None:intergenic
GAAGGAAGGGTGGAGGACCA+AGG 0.507927 4:-50606691 MsG0480020921.01.T01:CDS
AAGCACGAAGACAACAAAGA+AGG 0.514513 4:-50606723 MsG0480020921.01.T01:CDS
AAGGGAGCCGGAGGAAGGAA+GGG 0.514993 4:-50606704 MsG0480020921.01.T01:CDS
ACAGCCAACAACACATGCCT+TGG 0.515073 4:+50606464 None:intergenic
AGCACGAAGACAACAAAGAA+GGG 0.520726 4:-50606722 MsG0480020921.01.T01:CDS
ATCGGTCGATACCTGAAGAA+AGG 0.524114 4:-50606614 MsG0480020921.01.T01:CDS
GGCTGGAAAGTCTCCAAAGA+AGG 0.525629 4:-50606306 MsG0480020921.01.T01:CDS
TCTGGTGTCACAATTGCTCA+TGG 0.529253 4:-50606410 MsG0480020921.01.T01:CDS
GGAAGATACGCTCAGCGTGT+TGG 0.531317 4:-50606593 MsG0480020921.01.T01:CDS
TCCAACTGGAAATTGAAGAC+CGG 0.540370 4:+50606640 None:intergenic
AGCTAACAAAGAGCCAAAGA+AGG 0.544648 4:-50606327 MsG0480020921.01.T01:CDS
TTTCATTCTTCTTAGGCAAG+AGG 0.549215 4:+50606360 None:intergenic
AAGACAACAAAGAAGGGAGC+CGG 0.549880 4:-50606716 MsG0480020921.01.T01:CDS
CAAAGAAGGGAGCCGGAGGA+AGG 0.552147 4:-50606709 MsG0480020921.01.T01:CDS
GTTAGGAACGATGAAGAGCT+TGG 0.552797 4:-50606443 MsG0480020921.01.T01:CDS
ACAACAAAGAAGGGAGCCGG+AGG 0.563100 4:-50606713 MsG0480020921.01.T01:CDS
GCCGGTCTTCAATTTCCAGT+TGG 0.566234 4:-50606641 MsG0480020921.01.T01:CDS
GTGACAAGATCCATCAGAGC+CGG 0.589197 4:-50606659 MsG0480020921.01.T01:CDS
GATATTCAAGAACTGCTGCT+AGG 0.592671 4:+50606552 None:intergenic
AGAAGAATAGAATTATTCCA+AGG 0.617592 4:-50606481 MsG0480020921.01.T01:CDS
TGGAGGACCAAGGAAGAAGT+CGG 0.639719 4:-50606681 MsG0480020921.01.T01:CDS
GGTGTCACAATTGCTCATGG+TGG 0.701079 4:-50606407 MsG0480020921.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAGAATAGAATTATTCCA+AGG - Chr4:50606551-50606570 MsG0480020921.01.T01:CDS 25.0%
AAGAGGATAGGGTTAATGTT+TGG + Chr4:50606658-50606677 None:intergenic 35.0%
CAATTTCCAGTTGGAAGAAT+CGG - Chr4:50606400-50606419 MsG0480020921.01.T01:CDS 35.0%
GAGCTTGGTAAATTACTTTC+TGG - Chr4:50606604-50606623 MsG0480020921.01.T01:CDS 35.0%
TTTCATTCTTCTTAGGCAAG+AGG + Chr4:50606675-50606694 None:intergenic 35.0%
!!! GCAGCTTTTTCATTCTTCTT+AGG + Chr4:50606682-50606701 None:intergenic 35.0%
AAGCACGAAGACAACAAAGA+AGG - Chr4:50606309-50606328 MsG0480020921.01.T01:CDS 40.0%
AGCACGAAGACAACAAAGAA+GGG - Chr4:50606310-50606329 MsG0480020921.01.T01:CDS 40.0%
AGCTAACAAAGAGCCAAAGA+AGG - Chr4:50606705-50606724 MsG0480020921.01.T01:CDS 40.0%
GATATTCAAGAACTGCTGCT+AGG + Chr4:50606483-50606502 None:intergenic 40.0%
TATTCCAAGGCATGTGTTGT+TGG - Chr4:50606564-50606583 MsG0480020921.01.T01:CDS 40.0%
TCCAACTGGAAATTGAAGAC+CGG + Chr4:50606395-50606414 None:intergenic 40.0%
TCTTCTTAGGCAAGAGGATA+GGG + Chr4:50606669-50606688 None:intergenic 40.0%
TTCTTCTTAGGCAAGAGGAT+AGG + Chr4:50606670-50606689 None:intergenic 40.0%
!! TTCTTTGGCTCTTTGTTAGC+TGG + Chr4:50606706-50606725 None:intergenic 40.0%
AACAAAGAGCCAAAGAAGGC+TGG - Chr4:50606709-50606728 MsG0480020921.01.T01:CDS 45.0%
AAGACAACAAAGAAGGGAGC+CGG - Chr4:50606316-50606335 MsG0480020921.01.T01:CDS 45.0%
AGAACTGCTGCTAGGTATAC+TGG + Chr4:50606475-50606494 None:intergenic 45.0%
ATCGGTCGATACCTGAAGAA+AGG - Chr4:50606418-50606437 MsG0480020921.01.T01:CDS 45.0%
GAGCGTATCTTCCTTTCTTC+AGG + Chr4:50606432-50606451 None:intergenic 45.0%
GTTAGGAACGATGAAGAGCT+TGG - Chr4:50606589-50606608 MsG0480020921.01.T01:CDS 45.0%
TGCTGCTGAAGTTCTTGAGT+TGG - Chr4:50606504-50606523 MsG0480020921.01.T01:CDS 45.0%
! AAATTGAAGACCGGCTCTGA+TGG + Chr4:50606386-50606405 None:intergenic 45.0%
! TATCGACCGATTCTTCCAAC+TGG + Chr4:50606409-50606428 None:intergenic 45.0%
!! TCTGGTGTCACAATTGCTCA+TGG - Chr4:50606622-50606641 MsG0480020921.01.T01:CDS 45.0%
ACAGCCAACAACACATGCCT+TGG + Chr4:50606571-50606590 None:intergenic 50.0%
GCCGGTCTTCAATTTCCAGT+TGG - Chr4:50606391-50606410 MsG0480020921.01.T01:CDS 50.0%
GGAGACTTTCCAGCCTTCTT+TGG + Chr4:50606721-50606740 None:intergenic 50.0%
GGCTGGAAAGTCTCCAAAGA+AGG - Chr4:50606726-50606745 MsG0480020921.01.T01:CDS 50.0%
GTGACAAGATCCATCAGAGC+CGG - Chr4:50606373-50606392 MsG0480020921.01.T01:CDS 50.0%
TGGAGGACCAAGGAAGAAGT+CGG - Chr4:50606351-50606370 MsG0480020921.01.T01:CDS 50.0%
! GGCATGTGTTGTTGGCTGTT+AGG - Chr4:50606572-50606591 MsG0480020921.01.T01:CDS 50.0%
! GGTGTCACAATTGCTCATGG+TGG - Chr4:50606625-50606644 MsG0480020921.01.T01:CDS 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG + Chr4:50606361-50606380 None:intergenic 50.0%
!! GCTGAAGTTCTTGAGTTGGC+TGG - Chr4:50606508-50606527 MsG0480020921.01.T01:CDS 50.0%
ACAACAAAGAAGGGAGCCGG+AGG - Chr4:50606319-50606338 MsG0480020921.01.T01:CDS 55.0%
GGAAGATACGCTCAGCGTGT+TGG - Chr4:50606439-50606458 MsG0480020921.01.T01:CDS 55.0%
TACGCTCAGCGTGTTGGAAC+AGG - Chr4:50606445-50606464 MsG0480020921.01.T01:CDS 55.0%
AAGGGAGCCGGAGGAAGGAA+GGG - Chr4:50606328-50606347 MsG0480020921.01.T01:CDS 60.0%
CAAAGAAGGGAGCCGGAGGA+AGG - Chr4:50606323-50606342 MsG0480020921.01.T01:CDS 60.0%
GAAGGAAGGGTGGAGGACCA+AGG - Chr4:50606341-50606360 MsG0480020921.01.T01:CDS 60.0%
TCCTCCACCCTTCCTTCCTC+CGG + Chr4:50606338-50606357 None:intergenic 60.0%
GAAGGGAGCCGGAGGAAGGA+AGG - Chr4:50606327-50606346 MsG0480020921.01.T01:CDS 65.0%
GCCGGAGGAAGGAAGGGTGG+AGG - Chr4:50606334-50606353 MsG0480020921.01.T01:CDS 70.0%
GGAGCCGGAGGAAGGAAGGG+TGG - Chr4:50606331-50606350 MsG0480020921.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 50606301 50606753 50606301 ID=MsG0480020921.01;Name=MsG0480020921.01
Chr4 mRNA 50606301 50606753 50606301 ID=MsG0480020921.01.T01;Parent=MsG0480020921.01;Name=MsG0480020921.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|150
Chr4 exon 50606301 50606753 50606301 ID=MsG0480020921.01.T01:exon:326;Parent=MsG0480020921.01.T01
Chr4 CDS 50606301 50606753 50606301 ID=MsG0480020921.01.T01:cds;Parent=MsG0480020921.01.T01
Gene Sequence

>MsG0480020921.01.T01

ATGGACGCAAGCACGAAGACAACAAAGAAGGGAGCCGGAGGAAGGAAGGGTGGAGGACCAAGGAAGAAGTCGGTGACAAGATCCATCAGAGCCGGTCTTCAATTTCCAGTTGGAAGAATCGGTCGATACCTGAAGAAAGGAAGATACGCTCAGCGTGTTGGAACAGGTGCACCAGTATACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAAGTTCTTGAGTTGGCTGGAAATGCAGCTCGTGATAACAAGAAGAATAGAATTATTCCAAGGCATGTGTTGTTGGCTGTTAGGAACGATGAAGAGCTTGGTAAATTACTTTCTGGTGTCACAATTGCTCATGGTGGTGTTCTTCCAAACATTAACCCTATCCTCTTGCCTAAGAAGAATGAAAAAGCTGCTCCAGCTAACAAAGAGCCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MsG0480020921.01.T01

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLSGVTIAHGGVLPNINPILLPKKNEKAAPANKEPKKAGKSPKKA*