AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020926.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020926.01.T01 MTR_2g096570 94.737 152 4 3 1 149 1 151 1.06e-94 270
MsG0480020926.01.T01 MTR_4g063280 97.163 141 4 0 9 149 3 143 2.23e-93 267
MsG0480020926.01.T01 MTR_2g096610 88.591 149 14 2 1 149 1 146 1.13e-81 237
MsG0480020926.01.T01 MTR_4g071150 94.040 151 6 2 1 149 1 150 1.16e-81 237
MsG0480020926.01.T01 MTR_4g064005 93.960 149 6 1 1 149 1 146 2.35e-81 236
MsG0480020926.01.T01 MTR_4g063410 93.289 149 7 1 1 149 1 146 6.11e-81 235
MsG0480020926.01.T01 MTR_4g064967 93.960 149 6 1 1 149 1 146 1.45e-80 234
MsG0480020926.01.T01 MTR_8g086640 97.727 132 3 0 1 132 1 132 4.80e-76 223
MsG0480020926.01.T01 MTR_7g113160 82.090 134 22 2 10 143 13 144 5.45e-72 213
MsG0480020926.01.T01 MTR_7g108320 74.615 130 27 3 4 132 6 130 3.39e-60 182
MsG0480020926.01.T01 MTR_7g114060 73.846 130 30 1 3 132 2 127 4.18e-60 182
MsG0480020926.01.T01 MTR_7g114040 73.846 130 30 1 3 132 2 127 4.18e-60 182
MsG0480020926.01.T01 MTR_7g093910 72.441 127 32 1 4 130 5 128 2.93e-59 180
MsG0480020926.01.T01 MTR_2g082510 74.790 119 30 0 11 129 3 121 1.05e-58 179
MsG0480020926.01.T01 MTR_2g082220 73.950 119 31 0 11 129 3 121 1.24e-57 176
MsG0480020926.01.T01 MTR_6g005150 66.393 122 31 3 24 143 26 139 4.69e-48 152
MsG0480020926.01.T01 MTR_4g063300 80.000 105 15 1 45 149 10 108 1.05e-47 150
MsG0480020926.01.T01 MTR_7g114050 68.333 120 23 3 17 132 10 118 9.57e-46 145
MsG0480020926.01.T01 MTR_1g011750 59.292 113 44 2 21 132 23 134 9.91e-36 120
MsG0480020926.01.T01 MTR_1g011850 59.292 113 44 2 21 132 23 134 9.91e-36 120
MsG0480020926.01.T01 MTR_1g114050 59.633 109 42 2 21 128 20 127 8.20e-35 118
MsG0480020926.01.T01 MTR_1g071040 61.538 104 38 2 26 128 28 130 4.84e-34 116
MsG0480020926.01.T01 MTR_1g069770 52.174 92 43 1 45 135 9 100 1.58e-25 94.0
MsG0480020926.01.T01 MTR_7g010440 70.588 51 15 0 78 128 122 172 1.13e-16 75.1
MsG0480020926.01.T01 MTR_4g005910 74.419 43 11 0 87 129 4 46 1.88e-16 69.3
MsG0480020926.01.T01 MTR_4g048490 43.810 105 30 2 24 128 26 101 4.65e-16 69.7
MsG0480020926.01.T01 MTR_7g027610 41.818 110 38 3 20 128 10 94 4.47e-15 67.4
MsG0480020926.01.T01 MTR_0653s0030 53.704 54 25 0 56 109 44 97 1.34e-11 59.3
MsG0480020926.01.T01 MTR_2g096590 72.500 40 10 1 110 149 12 50 5.76e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020926.01.T01 AT5G02560 84.106 151 22 1 1 149 1 151 1.53e-80 234
MsG0480020926.01.T01 AT5G02560 72.571 175 22 2 1 149 1 175 1.88e-75 223
MsG0480020926.01.T01 AT5G27670 74.834 151 33 3 1 149 1 148 9.56e-73 215
MsG0480020926.01.T01 AT5G59870 75.676 148 29 3 4 144 3 150 1.39e-71 212
MsG0480020926.01.T01 AT1G51060 76.667 120 28 0 11 130 3 122 4.05e-61 185
MsG0480020926.01.T01 AT4G27230 75.630 119 29 0 11 129 3 121 1.17e-59 181
MsG0480020926.01.T01 AT4G27230 75.630 119 29 0 11 129 3 121 1.17e-59 181
MsG0480020926.01.T01 AT3G20670 75.630 119 29 0 11 129 3 121 3.20e-59 180
MsG0480020926.01.T01 AT1G08880 71.318 129 34 1 7 132 2 130 1.48e-58 179
MsG0480020926.01.T01 AT5G54640 74.790 119 30 0 11 129 3 121 2.20e-58 178
MsG0480020926.01.T01 AT1G54690 72.519 131 30 3 3 132 5 130 5.77e-58 177
MsG0480020926.01.T01 AT2G38810 59.821 112 43 2 21 131 25 135 2.40e-36 122
MsG0480020926.01.T01 AT2G38810 59.821 112 43 2 21 131 25 135 2.40e-36 122
MsG0480020926.01.T01 AT2G38810 59.821 112 43 2 21 131 25 135 2.40e-36 122
MsG0480020926.01.T01 AT2G38810 59.821 112 43 2 21 131 25 135 2.40e-36 122
MsG0480020926.01.T01 AT1G52740 59.633 109 42 2 25 132 27 134 1.58e-34 117
MsG0480020926.01.T01 AT3G54560 58.716 109 43 2 25 132 29 136 2.53e-34 117
MsG0480020926.01.T01 AT3G54560 58.716 109 43 2 25 132 29 136 2.53e-34 117
MsG0480020926.01.T01 AT4G13570 47.826 92 46 2 32 122 28 118 7.12e-20 79.7
MsG0480020926.01.T01 AT4G13570 47.253 91 46 2 33 122 35 124 1.81e-19 79.0

Find 45 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCCTTTCTTTGTGGTTT+TGG 0.155065 4:+50632627 None:intergenic
TCCTCCTGCTCCTTTCTTTG+TGG 0.311932 4:+50632621 None:intergenic
GACTGAAAAGGCTGCTTCTA+AGG 0.313763 4:-50632254 MsG0480020926.01.T01:CDS
CTTGTCACCGACTTCTTCCT+TGG 0.333455 4:+50632583 None:intergenic
AGAACTGCTGCAAGGTATAC+TGG 0.342117 4:+50632469 None:intergenic
CGATTCTTCCCACTGGGAAC+TGG 0.359689 4:+50632542 None:intergenic
TCTTCTTAGGCAAGAGAATA+GGG 0.366947 4:+50632275 None:intergenic
AAAGGAGCAGGAGGAAGGAA+GGG 0.396180 4:-50632613 MsG0480020926.01.T01:CDS
GTCTTCTTAGGCAAGAGAAT+AGG 0.408927 4:+50632274 None:intergenic
TACGCTCAGCGTGTTGGAAC+CGG 0.422732 4:-50632496 MsG0480020926.01.T01:CDS
CGCTGCTGAGGTTCTTGAGT+TGG 0.426254 4:-50632437 MsG0480020926.01.T01:CDS
AAGAATCAGATCTAAGCCTT+TGG 0.432647 4:+50632199 None:intergenic
AAGAGAATAGGGTTAATGTT+TGG 0.433482 4:+50632286 None:intergenic
GGAGCAGGAGGAAGGAAGGG+AGG 0.436317 4:-50632610 MsG0480020926.01.T01:CDS
GAAAGGAGCAGGAGGAAGGA+AGG 0.440519 4:-50632614 MsG0480020926.01.T01:CDS
GGCATGTGTTGTTGGCTGTT+AGG 0.442957 4:-50632369 MsG0480020926.01.T01:CDS
CATTCCAAGGCATGTGTTGT+TGG 0.459643 4:-50632377 MsG0480020926.01.T01:CDS
GCAGGAGGAAGGAAGGGAGG+AGG 0.468760 4:-50632607 MsG0480020926.01.T01:CDS
GCTGAGGTTCTTGAGTTGGC+TGG 0.475814 4:-50632433 MsG0480020926.01.T01:CDS
CAGTTCCCAGTGGGAAGAAT+CGG 0.476269 4:-50632541 MsG0480020926.01.T01:CDS
AGCACCAAAACCACAAAGAA+AGG 0.482345 4:-50632631 MsG0480020926.01.T01:CDS
CAAAGAAAGGAGCAGGAGGA+AGG 0.496497 4:-50632618 MsG0480020926.01.T01:CDS
GAGCTTGGTAAATTACTTGC+TGG 0.499780 4:-50632337 MsG0480020926.01.T01:CDS
ACAGCCAACAACACATGCCT+TGG 0.515073 4:+50632373 None:intergenic
ATCGGTCGATACTTGAAGAA+AGG 0.519021 4:-50632523 MsG0480020926.01.T01:CDS
AAAACCACAAAGAAAGGAGC+AGG 0.530917 4:-50632625 MsG0480020926.01.T01:CDS
GGAAGATACGCTCAGCGTGT+TGG 0.531317 4:-50632502 MsG0480020926.01.T01:CDS
GAAGGAAGGGAGGAGGACCA+AGG 0.532633 4:-50632600 MsG0480020926.01.T01:CDS
CTTGCCTAAGAAGACTGAAA+AGG 0.532710 4:-50632266 MsG0480020926.01.T01:CDS
TATCGACCGATTCTTCCCAC+TGG 0.544173 4:+50632535 None:intergenic
GTTAGGAACGATGAAGAGCT+TGG 0.552797 4:-50632352 MsG0480020926.01.T01:CDS
TCCCACTGGGAACTGGAGAC+CGG 0.556836 4:+50632549 None:intergenic
ACCACAAAGAAAGGAGCAGG+AGG 0.568964 4:-50632622 MsG0480020926.01.T01:CDS
TGCAAGGTATACTGGAGCAC+CGG 0.587239 4:+50632477 None:intergenic
GTGACAAGATCAATCAGAGC+CGG 0.601599 4:-50632568 MsG0480020926.01.T01:CDS
TCTTGAATATCTCGCTGCTG+AGG 0.603455 4:-50632449 MsG0480020926.01.T01:CDS
AGGAGGACCAAGGAAGAAGT+CGG 0.610829 4:-50632590 MsG0480020926.01.T01:CDS
GCCGGTCTCCAGTTCCCAGT+GGG 0.615696 4:-50632550 MsG0480020926.01.T01:CDS
AGAAGAATAGAATCATTCCA+AGG 0.617592 4:-50632390 MsG0480020926.01.T01:CDS
AAAAGCTGGAAAGTCTCCAA+AGG 0.624560 4:-50632215 MsG0480020926.01.T01:CDS
ATCGACCGATTCTTCCCACT+GGG 0.626884 4:+50632536 None:intergenic
GCTGGTGTTACAATTGCTCA+CGG 0.638494 4:-50632319 MsG0480020926.01.T01:CDS
GATATTCAAGAACTGCTGCA+AGG 0.659467 4:+50632461 None:intergenic
AGCCGGTCTCCAGTTCCCAG+TGG 0.691656 4:-50632551 MsG0480020926.01.T01:CDS
GGTGTTACAATTGCTCACGG+TGG 0.811864 4:-50632316 MsG0480020926.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGAGAATAGGGTTAATGTT+TGG + Chr4:50632567-50632586 None:intergenic 30.0%
AGAAGAATAGAATCATTCCA+AGG - Chr4:50632460-50632479 MsG0480020926.01.T01:CDS 30.0%
TCTTCTTAGGCAAGAGAATA+GGG + Chr4:50632578-50632597 None:intergenic 35.0%
AAAACCACAAAGAAAGGAGC+AGG - Chr4:50632225-50632244 MsG0480020926.01.T01:CDS 40.0%
AAAAGCTGGAAAGTCTCCAA+AGG - Chr4:50632635-50632654 MsG0480020926.01.T01:CDS 40.0%
AGCACCAAAACCACAAAGAA+AGG - Chr4:50632219-50632238 MsG0480020926.01.T01:CDS 40.0%
ATCGGTCGATACTTGAAGAA+AGG - Chr4:50632327-50632346 MsG0480020926.01.T01:CDS 40.0%
CTTGCCTAAGAAGACTGAAA+AGG - Chr4:50632584-50632603 MsG0480020926.01.T01:CDS 40.0%
GAGCTTGGTAAATTACTTGC+TGG - Chr4:50632513-50632532 MsG0480020926.01.T01:CDS 40.0%
GATATTCAAGAACTGCTGCA+AGG + Chr4:50632392-50632411 None:intergenic 40.0%
GTCTTCTTAGGCAAGAGAAT+AGG + Chr4:50632579-50632598 None:intergenic 40.0%
! TGCTCCTTTCTTTGTGGTTT+TGG + Chr4:50632226-50632245 None:intergenic 40.0%
!!! GGAGACTTTCCAGCTTTTTT+GGG + Chr4:50632633-50632652 None:intergenic 40.0%
!!! TGGAGACTTTCCAGCTTTTT+TGG + Chr4:50632634-50632653 None:intergenic 40.0%
AGAACTGCTGCAAGGTATAC+TGG + Chr4:50632384-50632403 None:intergenic 45.0%
CATTCCAAGGCATGTGTTGT+TGG - Chr4:50632473-50632492 MsG0480020926.01.T01:CDS 45.0%
CCTAAGTCTCCCAAAAAAGC+TGG - Chr4:50632621-50632640 MsG0480020926.01.T01:CDS 45.0%
GACTGAAAAGGCTGCTTCTA+AGG - Chr4:50632596-50632615 MsG0480020926.01.T01:CDS 45.0%
GTGACAAGATCAATCAGAGC+CGG - Chr4:50632282-50632301 MsG0480020926.01.T01:CDS 45.0%
GTTAGGAACGATGAAGAGCT+TGG - Chr4:50632498-50632517 MsG0480020926.01.T01:CDS 45.0%
TCTTGAATATCTCGCTGCTG+AGG - Chr4:50632401-50632420 MsG0480020926.01.T01:CDS 45.0%
! GCAGCCTTTTCAGTCTTCTT+AGG + Chr4:50632591-50632610 None:intergenic 45.0%
!! GCTGGTGTTACAATTGCTCA+CGG - Chr4:50632531-50632550 MsG0480020926.01.T01:CDS 45.0%
!!! CCAGCTTTTTTGGGAGACTT+AGG + Chr4:50632624-50632643 None:intergenic 45.0%
AAAGGAGCAGGAGGAAGGAA+GGG - Chr4:50632237-50632256 MsG0480020926.01.T01:CDS 50.0%
ACAGCCAACAACACATGCCT+TGG + Chr4:50632480-50632499 None:intergenic 50.0%
ACCACAAAGAAAGGAGCAGG+AGG - Chr4:50632228-50632247 MsG0480020926.01.T01:CDS 50.0%
AGGAGGACCAAGGAAGAAGT+CGG - Chr4:50632260-50632279 MsG0480020926.01.T01:CDS 50.0%
CAAAGAAAGGAGCAGGAGGA+AGG - Chr4:50632232-50632251 MsG0480020926.01.T01:CDS 50.0%
CAGTTCCCAGTGGGAAGAAT+CGG - Chr4:50632309-50632328 MsG0480020926.01.T01:CDS 50.0%
TCCTCCTGCTCCTTTCTTTG+TGG + Chr4:50632232-50632251 None:intergenic 50.0%
TGCAAGGTATACTGGAGCAC+CGG + Chr4:50632376-50632395 None:intergenic 50.0%
! ATCGACCGATTCTTCCCACT+GGG + Chr4:50632317-50632336 None:intergenic 50.0%
! GGCATGTGTTGTTGGCTGTT+AGG - Chr4:50632481-50632500 MsG0480020926.01.T01:CDS 50.0%
! GGTGTTACAATTGCTCACGG+TGG - Chr4:50632534-50632553 MsG0480020926.01.T01:CDS 50.0%
! TATCGACCGATTCTTCCCAC+TGG + Chr4:50632318-50632337 None:intergenic 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG + Chr4:50632270-50632289 None:intergenic 50.0%
CGATTCTTCCCACTGGGAAC+TGG + Chr4:50632311-50632330 None:intergenic 55.0%
CGCTGCTGAGGTTCTTGAGT+TGG - Chr4:50632413-50632432 MsG0480020926.01.T01:CDS 55.0%
GAAAGGAGCAGGAGGAAGGA+AGG - Chr4:50632236-50632255 MsG0480020926.01.T01:CDS 55.0%
GGAAGATACGCTCAGCGTGT+TGG - Chr4:50632348-50632367 MsG0480020926.01.T01:CDS 55.0%
TACGCTCAGCGTGTTGGAAC+CGG - Chr4:50632354-50632373 MsG0480020926.01.T01:CDS 55.0%
!! GCTGAGGTTCTTGAGTTGGC+TGG - Chr4:50632417-50632436 MsG0480020926.01.T01:CDS 55.0%
GAAGGAAGGGAGGAGGACCA+AGG - Chr4:50632250-50632269 MsG0480020926.01.T01:CDS 60.0%
TCCCACTGGGAACTGGAGAC+CGG + Chr4:50632304-50632323 None:intergenic 60.0%
AGCCGGTCTCCAGTTCCCAG+TGG - Chr4:50632299-50632318 MsG0480020926.01.T01:CDS 65.0%
GCAGGAGGAAGGAAGGGAGG+AGG - Chr4:50632243-50632262 MsG0480020926.01.T01:CDS 65.0%
GCCGGTCTCCAGTTCCCAGT+GGG - Chr4:50632300-50632319 MsG0480020926.01.T01:CDS 65.0%
GGAGCAGGAGGAAGGAAGGG+AGG - Chr4:50632240-50632259 MsG0480020926.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 50632210 50632662 50632210 ID=MsG0480020926.01;Name=MsG0480020926.01
Chr4 mRNA 50632210 50632662 50632210 ID=MsG0480020926.01.T01;Parent=MsG0480020926.01;Name=MsG0480020926.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|150
Chr4 exon 50632210 50632662 50632210 ID=MsG0480020926.01.T01:exon:328;Parent=MsG0480020926.01.T01
Chr4 CDS 50632210 50632662 50632210 ID=MsG0480020926.01.T01:cds;Parent=MsG0480020926.01.T01
Gene Sequence

>MsG0480020926.01.T01

ATGGACGCAAGCACCAAAACCACAAAGAAAGGAGCAGGAGGAAGGAAGGGAGGAGGACCAAGGAAGAAGTCGGTGACAAGATCAATCAGAGCCGGTCTCCAGTTCCCAGTGGGAAGAATCGGTCGATACTTGAAGAAAGGAAGATACGCTCAGCGTGTTGGAACCGGTGCTCCAGTATACCTTGCAGCAGTTCTTGAATATCTCGCTGCTGAGGTTCTTGAGTTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATAGAATCATTCCAAGGCATGTGTTGTTGGCTGTTAGGAACGATGAAGAGCTTGGTAAATTACTTGCTGGTGTTACAATTGCTCACGGTGGTGTTCTTCCAAACATTAACCCTATTCTCTTGCCTAAGAAGACTGAAAAGGCTGCTTCTAAGGAACCTAAGTCTCCCAAAAAAGCTGGAAAGTCTCCAAAGGCTTAG

Protein sequence

>MsG0480020926.01.T01

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTEKAASKEPKSPKKAGKSPKA*