AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020970.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020970.01.T01 MTR_2g096610 89.262 149 15 1 1 149 1 148 6.48e-84 243
MsG0480020970.01.T01 MTR_4g063280 93.939 132 7 1 19 149 14 145 8.14e-82 238
MsG0480020970.01.T01 MTR_4g071150 91.447 152 10 2 1 149 1 152 2.27e-81 236
MsG0480020970.01.T01 MTR_2g096570 88.235 153 14 1 1 149 1 153 3.02e-81 236
MsG0480020970.01.T01 MTR_4g064967 90.667 150 11 2 1 149 1 148 9.20e-81 235
MsG0480020970.01.T01 MTR_4g064005 91.333 150 10 2 1 149 1 148 9.20e-80 232
MsG0480020970.01.T01 MTR_4g063410 90.000 150 12 2 1 149 1 148 4.18e-79 231
MsG0480020970.01.T01 MTR_8g086640 92.424 132 9 1 1 131 1 132 2.07e-74 219
MsG0480020970.01.T01 MTR_7g113160 81.061 132 25 0 10 141 13 144 3.24e-73 216
MsG0480020970.01.T01 MTR_7g108320 72.868 129 34 1 4 131 2 130 3.19e-60 183
MsG0480020970.01.T01 MTR_7g093910 73.016 126 34 0 3 128 2 127 1.51e-59 181
MsG0480020970.01.T01 MTR_7g114060 73.643 129 31 1 3 131 2 127 1.71e-59 181
MsG0480020970.01.T01 MTR_7g114040 73.643 129 31 1 3 131 2 127 1.71e-59 181
MsG0480020970.01.T01 MTR_2g082510 77.477 111 25 0 18 128 11 121 8.02e-58 176
MsG0480020970.01.T01 MTR_2g082220 78.704 108 23 0 21 128 14 121 2.71e-57 175
MsG0480020970.01.T01 MTR_6g005150 63.780 127 32 3 23 148 26 139 3.09e-47 150
MsG0480020970.01.T01 MTR_7g114050 68.067 119 27 2 13 131 11 118 3.93e-46 147
MsG0480020970.01.T01 MTR_4g063300 75.238 105 21 1 44 148 10 109 1.70e-45 144
MsG0480020970.01.T01 MTR_1g114050 56.557 122 51 2 7 127 7 127 5.79e-36 121
MsG0480020970.01.T01 MTR_1g011750 59.292 113 44 2 20 131 23 134 7.95e-36 120
MsG0480020970.01.T01 MTR_1g011850 59.292 113 44 2 20 131 23 134 7.95e-36 120
MsG0480020970.01.T01 MTR_1g071040 61.538 104 38 2 25 127 28 130 3.97e-34 116
MsG0480020970.01.T01 MTR_1g069770 50.000 96 47 1 44 138 9 104 1.05e-25 94.4
MsG0480020970.01.T01 MTR_7g010440 70.588 51 15 0 77 127 122 172 1.29e-16 74.7
MsG0480020970.01.T01 MTR_4g005910 74.419 43 11 0 86 128 4 46 2.02e-16 69.3
MsG0480020970.01.T01 MTR_4g048490 43.810 105 30 2 23 127 26 101 3.81e-16 69.7
MsG0480020970.01.T01 MTR_7g027610 41.818 110 38 3 19 127 10 94 4.08e-15 67.4
MsG0480020970.01.T01 MTR_2g096590 75.000 40 10 0 109 148 12 51 7.83e-13 59.7
MsG0480020970.01.T01 MTR_0653s0030 53.704 54 25 0 55 108 44 97 1.28e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020970.01.T01 AT5G02560 81.046 153 25 2 1 149 1 153 4.84e-77 226
MsG0480020970.01.T01 AT5G27670 75.497 151 34 2 1 149 1 150 1.40e-74 219
MsG0480020970.01.T01 AT5G02560 70.056 177 25 3 1 149 1 177 3.54e-72 214
MsG0480020970.01.T01 AT5G59870 74.324 148 36 1 4 149 3 150 1.89e-71 211
MsG0480020970.01.T01 AT1G51060 74.380 121 31 0 18 138 11 131 1.08e-60 184
MsG0480020970.01.T01 AT4G27230 72.727 121 33 0 18 138 11 131 3.11e-59 180
MsG0480020970.01.T01 AT4G27230 72.727 121 33 0 18 138 11 131 3.11e-59 180
MsG0480020970.01.T01 AT3G20670 73.554 121 32 0 18 138 11 131 3.83e-59 180
MsG0480020970.01.T01 AT1G08880 73.600 125 29 1 11 131 6 130 1.19e-58 179
MsG0480020970.01.T01 AT1G54690 73.600 125 29 1 11 131 6 130 1.90e-58 178
MsG0480020970.01.T01 AT5G54640 74.359 117 30 0 18 134 11 127 1.25e-57 176
MsG0480020970.01.T01 AT2G38810 58.621 116 46 2 16 130 21 135 1.74e-36 122
MsG0480020970.01.T01 AT2G38810 58.621 116 46 2 16 130 21 135 1.74e-36 122
MsG0480020970.01.T01 AT2G38810 58.621 116 46 2 16 130 21 135 1.74e-36 122
MsG0480020970.01.T01 AT2G38810 58.621 116 46 2 16 130 21 135 1.74e-36 122
MsG0480020970.01.T01 AT1G52740 59.633 109 42 2 24 131 27 134 1.43e-34 117
MsG0480020970.01.T01 AT3G54560 58.716 109 43 2 24 131 29 136 2.29e-34 117
MsG0480020970.01.T01 AT3G54560 58.716 109 43 2 24 131 29 136 2.29e-34 117
MsG0480020970.01.T01 AT4G13570 47.826 92 46 2 31 121 28 118 5.94e-20 80.1
MsG0480020970.01.T01 AT4G13570 47.253 91 46 2 32 121 35 124 1.49e-19 79.3

Find 43 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGACTTTCCAGCCTTCTT+TGG 0.239817 4:-51341164 None:intergenic
GATCAGATCTAAGCCTTCTT+TGG 0.251178 4:-51341185 None:intergenic
GGCTCCTTTCTTCGCGGTCT+TGG 0.319054 4:-51340763 None:intergenic
CTTGTCACCGACTTCTTCCT+TGG 0.333455 4:-51340804 None:intergenic
TGCTGCTGAAGTTCTTGAAT+TGG 0.339223 4:+51340950 MsG0480020970.01.T01:CDS
AGAACTGCTGCTAGGTATAC+TGG 0.360588 4:-51340918 None:intergenic
TTCTTTGGCTCTTTGTTAGC+AGG 0.375405 4:-51341149 None:intergenic
TCTTCTTAGGCAAGAGGATT+GGG 0.401488 4:-51341112 None:intergenic
GTCTTCTTAGGCAAGAGGAT+TGG 0.405495 4:-51341113 None:intergenic
AAGAGGATTGGGTTAATGTT+TGG 0.407916 4:-51341101 None:intergenic
CAATTTCCAGTCGGAAGAAT+CGG 0.409724 4:+51340846 MsG0480020970.01.T01:CDS
GGCATGTGTTGTTGGCTGTT+AGG 0.442957 4:+51341018 MsG0480020970.01.T01:CDS
AGCACCAAGACCGCGAAGAA+AGG 0.452810 4:+51340759 MsG0480020970.01.T01:CDS
GCTGAAGTTCTTGAATTGGC+TGG 0.461152 4:+51340954 MsG0480020970.01.T01:CDS
AACAAAGAGCCAAAGAAGGC+TGG 0.466354 4:+51341155 MsG0480020970.01.T01:CDS
TATTCCAAGGCATGTGTTGT+TGG 0.470522 4:+51341010 MsG0480020970.01.T01:CDS
AAAGGAGCCGGTGGAAGGAA+AGG 0.476592 4:+51340777 MsG0480020970.01.T01:CDS
TACGCTCAGCGTGTTGGTAC+CGG 0.482649 4:+51340891 MsG0480020970.01.T01:CDS
TGGTCCTCCTTTCCTTCCAC+CGG 0.483261 4:-51340784 None:intergenic
GAGCTTGGTAAATTACTTGC+TGG 0.499780 4:+51341050 MsG0480020970.01.T01:CDS
ACAGCCAACAACACATGCCT+TGG 0.515073 4:-51341014 None:intergenic
ATCGGTCGATACTTGAAGAA+AGG 0.519021 4:+51340864 MsG0480020970.01.T01:CDS
GGCTGGAAAGTCTCCAAAGA+AGG 0.525629 4:+51341172 MsG0480020970.01.T01:CDS
TCCACCGGCTCCTTTCTTCG+CGG 0.538173 4:-51340769 None:intergenic
TGCTAACAAAGAGCCAAAGA+AGG 0.544648 4:+51341151 MsG0480020970.01.T01:CDS
TCCGACTGGAAATTGAAGAC+CGG 0.546631 4:-51340838 None:intergenic
TTTCAGTCTTCTTAGGCAAG+AGG 0.549625 4:-51341118 None:intergenic
CGAAGAAAGGAGCCGGTGGA+AGG 0.552463 4:+51340772 MsG0480020970.01.T01:CDS
GTTAGGAACGATGAAGAGCT+TGG 0.552797 4:+51341035 MsG0480020970.01.T01:CDS
GCTGGTGTCACAATTGCTCA+TGG 0.556477 4:+51341068 MsG0480020970.01.T01:CDS
GGAAGATACGCTCAGCGTGT+TGG 0.585684 4:+51340885 MsG0480020970.01.T01:CDS
TATCGACCGATTCTTCCGAC+TGG 0.586938 4:-51340852 None:intergenic
AAGACCGCGAAGAAAGGAGC+CGG 0.590011 4:+51340765 MsG0480020970.01.T01:CDS
GATATTCAAGAACTGCTGCT+AGG 0.592671 4:-51340926 None:intergenic
TGCTAGGTATACTGGAGCAC+CGG 0.594421 4:-51340910 None:intergenic
GTGACAAGATCAATCAGAGC+CGG 0.601599 4:+51340819 MsG0480020970.01.T01:CDS
ACCGCGAAGAAAGGAGCCGG+TGG 0.603380 4:+51340768 MsG0480020970.01.T01:CDS
GCCGGTCTTCAATTTCCAGT+CGG 0.613739 4:+51340837 MsG0480020970.01.T01:CDS
AGAAGAATAGAATTATTCCA+AGG 0.617592 4:+51340997 MsG0480020970.01.T01:CDS
GGAGCCGGTGGAAGGAAAGG+AGG 0.629948 4:+51340780 MsG0480020970.01.T01:CDS
AGGAGGACCAAGGAAGAAGT+CGG 0.642719 4:+51340797 MsG0480020970.01.T01:CDS
GTGGAAGGAAAGGAGGACCA+AGG 0.686614 4:+51340787 MsG0480020970.01.T01:CDS
GGTGTCACAATTGCTCATGG+TGG 0.701079 4:+51341071 MsG0480020970.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAGAATAGAATTATTCCA+AGG + Chr4:51340997-51341016 MsG0480020970.01.T01:CDS 25.0%
AAGAGGATTGGGTTAATGTT+TGG - Chr4:51341104-51341123 None:intergenic 35.0%
ATCGGTCGATACTTGAAGAA+AGG + Chr4:51340864-51340883 MsG0480020970.01.T01:CDS 40.0%
CAATTTCCAGTCGGAAGAAT+CGG + Chr4:51340846-51340865 MsG0480020970.01.T01:CDS 40.0%
GAGCTTGGTAAATTACTTGC+TGG + Chr4:51341050-51341069 MsG0480020970.01.T01:CDS 40.0%
GATATTCAAGAACTGCTGCT+AGG - Chr4:51340929-51340948 None:intergenic 40.0%
TATTCCAAGGCATGTGTTGT+TGG + Chr4:51341010-51341029 MsG0480020970.01.T01:CDS 40.0%
TCTTCTTAGGCAAGAGGATT+GGG - Chr4:51341115-51341134 None:intergenic 40.0%
TGCTAACAAAGAGCCAAAGA+AGG + Chr4:51341151-51341170 MsG0480020970.01.T01:CDS 40.0%
TGCTGCTGAAGTTCTTGAAT+TGG + Chr4:51340950-51340969 MsG0480020970.01.T01:CDS 40.0%
TTTCAGTCTTCTTAGGCAAG+AGG - Chr4:51341121-51341140 None:intergenic 40.0%
!! TTCTTTGGCTCTTTGTTAGC+AGG - Chr4:51341152-51341171 None:intergenic 40.0%
!!! GCAGCTTTTTCAGTCTTCTT+AGG - Chr4:51341128-51341147 None:intergenic 40.0%
AACAAAGAGCCAAAGAAGGC+TGG + Chr4:51341155-51341174 MsG0480020970.01.T01:CDS 45.0%
AGAACTGCTGCTAGGTATAC+TGG - Chr4:51340921-51340940 None:intergenic 45.0%
GCTGAAGTTCTTGAATTGGC+TGG + Chr4:51340954-51340973 MsG0480020970.01.T01:CDS 45.0%
GTCTTCTTAGGCAAGAGGAT+TGG - Chr4:51341116-51341135 None:intergenic 45.0%
GTGACAAGATCAATCAGAGC+CGG + Chr4:51340819-51340838 MsG0480020970.01.T01:CDS 45.0%
GTTAGGAACGATGAAGAGCT+TGG + Chr4:51341035-51341054 MsG0480020970.01.T01:CDS 45.0%
TCCGACTGGAAATTGAAGAC+CGG - Chr4:51340841-51340860 None:intergenic 45.0%
ACAGCCAACAACACATGCCT+TGG - Chr4:51341017-51341036 None:intergenic 50.0%
AGGAGGACCAAGGAAGAAGT+CGG + Chr4:51340797-51340816 MsG0480020970.01.T01:CDS 50.0%
GCCGGTCTTCAATTTCCAGT+CGG + Chr4:51340837-51340856 MsG0480020970.01.T01:CDS 50.0%
GGAGACTTTCCAGCCTTCTT+TGG - Chr4:51341167-51341186 None:intergenic 50.0%
GGCTGGAAAGTCTCCAAAGA+AGG + Chr4:51341172-51341191 MsG0480020970.01.T01:CDS 50.0%
TGCTAGGTATACTGGAGCAC+CGG - Chr4:51340913-51340932 None:intergenic 50.0%
! GGCATGTGTTGTTGGCTGTT+AGG + Chr4:51341018-51341037 MsG0480020970.01.T01:CDS 50.0%
! GGTGTCACAATTGCTCATGG+TGG + Chr4:51341071-51341090 MsG0480020970.01.T01:CDS 50.0%
! TATCGACCGATTCTTCCGAC+TGG - Chr4:51340855-51340874 None:intergenic 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG - Chr4:51340807-51340826 None:intergenic 50.0%
!! GCTGGTGTCACAATTGCTCA+TGG + Chr4:51341068-51341087 MsG0480020970.01.T01:CDS 50.0%
AAAGGAGCCGGTGGAAGGAA+AGG + Chr4:51340777-51340796 MsG0480020970.01.T01:CDS 55.0%
AAGACCGCGAAGAAAGGAGC+CGG + Chr4:51340765-51340784 MsG0480020970.01.T01:CDS 55.0%
AGCACCAAGACCGCGAAGAA+AGG + Chr4:51340759-51340778 MsG0480020970.01.T01:CDS 55.0%
GGAAGATACGCTCAGCGTGT+TGG + Chr4:51340885-51340904 MsG0480020970.01.T01:CDS 55.0%
GTGGAAGGAAAGGAGGACCA+AGG + Chr4:51340787-51340806 MsG0480020970.01.T01:CDS 55.0%
TGGTCCTCCTTTCCTTCCAC+CGG - Chr4:51340787-51340806 None:intergenic 55.0%
! TACGCTCAGCGTGTTGGTAC+CGG + Chr4:51340891-51340910 MsG0480020970.01.T01:CDS 55.0%
CGAAGAAAGGAGCCGGTGGA+AGG + Chr4:51340772-51340791 MsG0480020970.01.T01:CDS 60.0%
GGCTCCTTTCTTCGCGGTCT+TGG - Chr4:51340766-51340785 None:intergenic 60.0%
!! TCCACCGGCTCCTTTCTTCG+CGG - Chr4:51340772-51340791 None:intergenic 60.0%
ACCGCGAAGAAAGGAGCCGG+TGG + Chr4:51340768-51340787 MsG0480020970.01.T01:CDS 65.0%
GGAGCCGGTGGAAGGAAAGG+AGG + Chr4:51340780-51340799 MsG0480020970.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 51340750 51341199 51340750 ID=MsG0480020970.01;Name=MsG0480020970.01
Chr4 mRNA 51340750 51341199 51340750 ID=MsG0480020970.01.T01;Parent=MsG0480020970.01;Name=MsG0480020970.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|149
Chr4 exon 51340750 51341199 51340750 ID=MsG0480020970.01.T01:exon:1085;Parent=MsG0480020970.01.T01
Chr4 CDS 51340750 51341199 51340750 ID=MsG0480020970.01.T01:cds;Parent=MsG0480020970.01.T01
Gene Sequence

>MsG0480020970.01.T01

ATGGACGCAAGCACCAAGACCGCGAAGAAAGGAGCCGGTGGAAGGAAAGGAGGACCAAGGAAGAAGTCGGTGACAAGATCAATCAGAGCCGGTCTTCAATTTCCAGTCGGAAGAATCGGTCGATACTTGAAGAAAGGAAGATACGCTCAGCGTGTTGGTACCGGTGCTCCAGTATACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAAGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATAGAATTATTCCAAGGCATGTGTTGTTGGCTGTTAGGAACGATGAAGAGCTTGGTAAATTACTTGCTGGTGTCACAATTGCTCATGGTGGTGTTCTTCCAAACATTAACCCAATCCTCTTGCCTAAGAAGACTGAAAAAGCTGCTCCTGCTAACAAAGAGCCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MsG0480020970.01.T01

MDASTKTAKKGAGGRKGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTEKAAPANKEPKKAGKSPKKA*