AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041273.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041273.01.T01 MTR_7g108320 80.822 146 19 2 1 141 1 142 2.77e-73 215
MsG0780041273.01.T01 MTR_7g114060 78.322 143 25 2 1 141 1 139 5.28e-68 202
MsG0780041273.01.T01 MTR_7g114040 78.322 143 25 2 1 141 1 139 5.28e-68 202
MsG0780041273.01.T01 MTR_7g093910 85.437 103 15 0 39 141 40 142 3.50e-60 182
MsG0780041273.01.T01 MTR_2g082220 82.075 106 15 1 26 131 25 126 1.80e-53 165
MsG0780041273.01.T01 MTR_2g082510 83.495 103 13 1 26 128 25 123 2.41e-53 165
MsG0780041273.01.T01 MTR_7g114050 80.172 116 8 3 26 141 30 130 1.74e-52 162
MsG0780041273.01.T01 MTR_2g096610 80.682 88 17 0 39 126 40 127 7.67e-46 146
MsG0780041273.01.T01 MTR_2g096570 80.682 88 17 0 39 126 41 128 1.09e-45 146
MsG0780041273.01.T01 MTR_4g064005 80.682 88 17 0 39 126 42 129 1.80e-45 145
MsG0780041273.01.T01 MTR_4g063410 78.652 89 19 0 39 127 42 130 1.90e-45 145
MsG0780041273.01.T01 MTR_8g086640 80.682 88 17 0 39 126 42 129 2.06e-45 145
MsG0780041273.01.T01 MTR_4g071150 79.775 89 18 0 39 127 42 130 2.08e-45 145
MsG0780041273.01.T01 MTR_4g064967 80.682 88 17 0 39 126 42 129 3.95e-45 144
MsG0780041273.01.T01 MTR_4g063280 78.022 91 20 0 39 129 36 126 5.00e-45 144
MsG0780041273.01.T01 MTR_7g113160 75.281 89 22 0 39 127 44 132 1.19e-43 140
MsG0780041273.01.T01 MTR_4g063300 67.708 96 23 1 42 129 10 105 2.67e-36 121
MsG0780041273.01.T01 MTR_1g011750 49.630 135 53 5 1 125 1 130 5.37e-30 105
MsG0780041273.01.T01 MTR_1g011850 49.630 135 53 5 1 125 1 130 5.37e-30 105
MsG0780041273.01.T01 MTR_6g005150 58.333 96 31 2 39 133 44 131 1.24e-28 102
MsG0780041273.01.T01 MTR_1g071040 54.000 100 42 2 26 125 35 130 2.81e-28 101
MsG0780041273.01.T01 MTR_1g114050 46.565 131 60 4 1 125 1 127 4.35e-28 100
MsG0780041273.01.T01 MTR_4g005910 88.889 45 5 0 84 128 4 48 1.49e-22 84.7
MsG0780041273.01.T01 MTR_7g010440 68.182 66 21 0 75 140 122 187 4.41e-22 89.4
MsG0780041273.01.T01 MTR_1g069770 55.224 67 30 0 64 130 32 98 4.01e-19 77.4
MsG0780041273.01.T01 MTR_4g048490 57.576 66 27 1 62 127 39 103 1.45e-18 75.9
MsG0780041273.01.T01 MTR_0653s0030 52.830 53 25 0 52 104 43 95 5.89e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041273.01.T01 AT1G54690 73.973 146 29 2 1 141 1 142 1.45e-65 196
MsG0780041273.01.T01 AT1G08880 72.603 146 31 2 1 141 1 142 9.84e-65 194
MsG0780041273.01.T01 AT4G27230 82.075 106 15 1 26 131 25 126 7.67e-54 166
MsG0780041273.01.T01 AT4G27230 82.075 106 15 1 26 131 25 126 7.67e-54 166
MsG0780041273.01.T01 AT5G54640 82.075 106 15 1 26 131 25 126 1.51e-53 165
MsG0780041273.01.T01 AT1G51060 81.132 106 16 1 26 131 25 126 5.15e-53 164
MsG0780041273.01.T01 AT3G20670 80.189 106 17 1 26 131 25 126 5.47e-52 161
MsG0780041273.01.T01 AT5G59870 71.287 101 25 1 26 126 33 129 9.97e-45 144
MsG0780041273.01.T01 AT5G02560 78.409 88 19 0 39 126 42 129 2.17e-44 143
MsG0780041273.01.T01 AT5G27670 77.273 88 20 0 39 126 43 130 6.82e-43 139
MsG0780041273.01.T01 AT5G02560 61.607 112 19 1 39 126 42 153 3.66e-39 130
MsG0780041273.01.T01 AT1G52740 56.436 101 38 3 26 125 35 130 1.02e-27 100
MsG0780041273.01.T01 AT2G38810 62.338 77 28 1 49 125 57 132 2.29e-27 99.4
MsG0780041273.01.T01 AT2G38810 62.338 77 28 1 49 125 57 132 2.29e-27 99.4
MsG0780041273.01.T01 AT2G38810 62.338 77 28 1 49 125 57 132 2.29e-27 99.4
MsG0780041273.01.T01 AT2G38810 62.338 77 28 1 49 125 57 132 2.29e-27 99.4
MsG0780041273.01.T01 AT3G54560 62.338 77 28 1 49 125 57 132 2.56e-27 99.0
MsG0780041273.01.T01 AT3G54560 62.338 77 28 1 49 125 57 132 2.56e-27 99.0
MsG0780041273.01.T01 AT4G13570 54.286 70 31 1 50 119 50 118 1.03e-20 81.6
MsG0780041273.01.T01 AT4G13570 54.286 70 31 1 50 119 56 124 2.71e-20 80.9

Find 41 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATCTCGCTGCTGAGGTTT+TGG 0.254221 7:-89024683 MsG0780041273.01.T01:CDS
TGAAGAACTCAGCAAGCTTT+TGG 0.258146 7:-89024581 MsG0780041273.01.T01:CDS
AAGCAATGTCTGATGAATAT+TGG 0.288668 7:+89024525 None:intergenic
GAAACGGATTTGGAGGCTTT+TGG 0.298714 7:+89024824 None:intergenic
TGAAGATCTTGAAACGGATT+TGG 0.320293 7:+89024814 None:intergenic
GGAAAAGGGAAAGGTGAAAT+TGG 0.334923 7:-89024490 MsG0780041273.01.T01:CDS
TGTTGTAGTTGTGGCACCTT+TGG 0.379726 7:+89024864 None:intergenic
TCAGACATTGCTTCCTAAGA+AGG 0.403914 7:-89024515 MsG0780041273.01.T01:CDS
AGCAATGTCTGATGAATATT+GGG 0.409255 7:+89024526 None:intergenic
CTTCCTAAGAAGGTTGGAAA+AGG 0.418428 7:-89024505 MsG0780041273.01.T01:CDS
TTCCTCCTTTGTTGTAGTTG+TGG 0.435784 7:+89024855 None:intergenic
GCGTGTTGGCGCCGGTGCTC+CGG 0.455502 7:-89024728 MsG0780041273.01.T01:CDS
TACGCCGAGCGTGTTGGCGC+CGG 0.470776 7:-89024736 MsG0780041273.01.T01:CDS
AGATCTTGAAACGGATTTGG+AGG 0.479759 7:+89024817 None:intergenic
AGCACCGGCGCCAACACGCT+CGG 0.487757 7:+89024732 None:intergenic
ACATTGCTTCCTAAGAAGGT+TGG 0.502061 7:-89024511 MsG0780041273.01.T01:CDS
AATCGCAAGATTCTCAAGGC+TGG 0.513553 7:-89024763 MsG0780041273.01.T01:CDS
AACTACAACAAAGGAGGAAG+AGG 0.518091 7:-89024851 MsG0780041273.01.T01:CDS
GGAAAATACGCCGAGCGTGT+TGG 0.518396 7:-89024742 MsG0780041273.01.T01:CDS
TTCCGACGGGAATTGAAGAC+CGG 0.523339 7:+89024787 None:intergenic
GGTGCCGTAACCATTGCTAA+TGG 0.528391 7:-89024559 MsG0780041273.01.T01:CDS
TTCCTAAGAAGGTTGGAAAA+GGG 0.529191 7:-89024504 MsG0780041273.01.T01:CDS
GTTTCAAGATCTTCAAAAGC+CGG 0.540874 7:-89024806 MsG0780041273.01.T01:CDS
AGAGAGATAGACCGGAGCAC+CGG 0.550862 7:+89024717 None:intergenic
AGAACAGCAGAGAGATAGAC+CGG 0.553809 7:+89024709 None:intergenic
ACCACCATTAGCAATGGTTA+CGG 0.568022 7:+89024555 None:intergenic
ACAGCAAGCTGAATGTGTCT+CGG 0.587168 7:+89024613 None:intergenic
AGGTGCCACAACTACAACAA+AGG 0.596789 7:-89024860 MsG0780041273.01.T01:CDS
AATCTTGCGATTCTTCCGAC+GGG 0.601933 7:+89024774 None:intergenic
GAATCTTGCGATTCTTCCGA+CGG 0.609246 7:+89024773 None:intergenic
AAGAAGGTTGGAAAAGGGAA+AGG 0.622701 7:-89024499 MsG0780041273.01.T01:CDS
AATCCGAAAATGAGTTCCAA+AGG 0.625540 7:-89024880 None:intergenic
GCCGTAACCATTGCTAATGG+TGG 0.628392 7:-89024556 MsG0780041273.01.T01:CDS
GAAGAATCGCAAGATTCTCA+AGG 0.634136 7:-89024767 MsG0780041273.01.T01:CDS
CAAAACACCACCATTAGCAA+TGG 0.637204 7:+89024549 None:intergenic
TCTCGAGTATCTCGCTGCTG+AGG 0.640871 7:-89024689 MsG0780041273.01.T01:CDS
ATTGGCTGGTAATGCTGCGA+GGG 0.649014 7:-89024659 MsG0780041273.01.T01:CDS
AGCCGGTCTTCAATTCCCGT+CGG 0.659223 7:-89024789 MsG0780041273.01.T01:CDS
TGCCACAACTACAACAAAGG+AGG 0.668759 7:-89024857 MsG0780041273.01.T01:CDS
GACACATTCAGCTTGCTGTG+AGG 0.685856 7:-89024609 MsG0780041273.01.T01:CDS
AATTGGCTGGTAATGCTGCG+AGG 0.697250 7:-89024660 MsG0780041273.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGCAATGTCTGATGAATAT+TGG + Chr7:89024817-89024836 None:intergenic 30.0%
AGCAATGTCTGATGAATATT+GGG + Chr7:89024816-89024835 None:intergenic 30.0%
GTTTCAAGATCTTCAAAAGC+CGG - Chr7:89024533-89024552 MsG0780041273.01.T01:CDS 35.0%
TTCCTAAGAAGGTTGGAAAA+GGG - Chr7:89024835-89024854 MsG0780041273.01.T01:CDS 35.0%
! TGAAGATCTTGAAACGGATT+TGG + Chr7:89024528-89024547 None:intergenic 35.0%
!! GGCTTTTGAAGATCTTGAAA+CGG + Chr7:89024534-89024553 None:intergenic 35.0%
AACTACAACAAAGGAGGAAG+AGG - Chr7:89024488-89024507 MsG0780041273.01.T01:CDS 40.0%
AAGAAGGTTGGAAAAGGGAA+AGG - Chr7:89024840-89024859 MsG0780041273.01.T01:CDS 40.0%
ACATTGCTTCCTAAGAAGGT+TGG - Chr7:89024828-89024847 MsG0780041273.01.T01:CDS 40.0%
ACCACCATTAGCAATGGTTA+CGG + Chr7:89024787-89024806 None:intergenic 40.0%
CAAAACACCACCATTAGCAA+TGG + Chr7:89024793-89024812 None:intergenic 40.0%
CTTCCTAAGAAGGTTGGAAA+AGG - Chr7:89024834-89024853 MsG0780041273.01.T01:CDS 40.0%
GAAGAATCGCAAGATTCTCA+AGG - Chr7:89024572-89024591 MsG0780041273.01.T01:CDS 40.0%
GGAAAAGGGAAAGGTGAAAT+TGG - Chr7:89024849-89024868 MsG0780041273.01.T01:CDS 40.0%
TCAGACATTGCTTCCTAAGA+AGG - Chr7:89024824-89024843 MsG0780041273.01.T01:CDS 40.0%
! AGATCTTGAAACGGATTTGG+AGG + Chr7:89024525-89024544 None:intergenic 40.0%
! GAAGAACTCAGCAAGCTTTT+GGG - Chr7:89024759-89024778 MsG0780041273.01.T01:CDS 40.0%
! TGAAGAACTCAGCAAGCTTT+TGG - Chr7:89024758-89024777 MsG0780041273.01.T01:CDS 40.0%
! TTCCCTTTTCCAACCTTCTT+AGG + Chr7:89024840-89024859 None:intergenic 40.0%
! TTCCTCCTTTGTTGTAGTTG+TGG + Chr7:89024487-89024506 None:intergenic 40.0%
AATCGCAAGATTCTCAAGGC+TGG - Chr7:89024576-89024595 MsG0780041273.01.T01:CDS 45.0%
AATCTTGCGATTCTTCCGAC+GGG + Chr7:89024568-89024587 None:intergenic 45.0%
ACAGCAAGCTGAATGTGTCT+CGG + Chr7:89024729-89024748 None:intergenic 45.0%
AGAACAGCAGAGAGATAGAC+CGG + Chr7:89024633-89024652 None:intergenic 45.0%
GAATCTTGCGATTCTTCCGA+CGG + Chr7:89024569-89024588 None:intergenic 45.0%
TGCCACAACTACAACAAAGG+AGG - Chr7:89024482-89024501 MsG0780041273.01.T01:CDS 45.0%
TGTTGTAGTTGTGGCACCTT+TGG + Chr7:89024478-89024497 None:intergenic 45.0%
! AGGTGCCACAACTACAACAA+AGG - Chr7:89024479-89024498 MsG0780041273.01.T01:CDS 45.0%
!! GAAACGGATTTGGAGGCTTT+TGG + Chr7:89024518-89024537 None:intergenic 45.0%
GCCGTAACCATTGCTAATGG+TGG - Chr7:89024783-89024802 MsG0780041273.01.T01:CDS 50.0%
TTCCGACGGGAATTGAAGAC+CGG + Chr7:89024555-89024574 None:intergenic 50.0%
! AATTGGCTGGTAATGCTGCG+AGG - Chr7:89024679-89024698 MsG0780041273.01.T01:CDS 50.0%
! ATTGGCTGGTAATGCTGCGA+GGG - Chr7:89024680-89024699 MsG0780041273.01.T01:CDS 50.0%
! GGTGCCGTAACCATTGCTAA+TGG - Chr7:89024780-89024799 MsG0780041273.01.T01:CDS 50.0%
! GTATCTCGCTGCTGAGGTTT+TGG - Chr7:89024656-89024675 MsG0780041273.01.T01:CDS 50.0%
!! GACACATTCAGCTTGCTGTG+AGG - Chr7:89024730-89024749 MsG0780041273.01.T01:CDS 50.0%
!!! CGCTGCTGAGGTTTTGGAAT+TGG - Chr7:89024662-89024681 MsG0780041273.01.T01:CDS 50.0%
!!! GCTGAGGTTTTGGAATTGGC+TGG - Chr7:89024666-89024685 MsG0780041273.01.T01:CDS 50.0%
AGAGAGATAGACCGGAGCAC+CGG + Chr7:89024625-89024644 None:intergenic 55.0%
AGCCGGTCTTCAATTCCCGT+CGG - Chr7:89024550-89024569 MsG0780041273.01.T01:CDS 55.0%
GGAAAATACGCCGAGCGTGT+TGG - Chr7:89024597-89024616 MsG0780041273.01.T01:CDS 55.0%
TCTCGAGTATCTCGCTGCTG+AGG - Chr7:89024650-89024669 MsG0780041273.01.T01:CDS 55.0%
! TACGCCGAGCGTGTTGGCGC+CGG - Chr7:89024603-89024622 MsG0780041273.01.T01:CDS 70.0%
!! AGCACCGGCGCCAACACGCT+CGG + Chr7:89024610-89024629 None:intergenic 70.0%
!! GCGTGTTGGCGCCGGTGCTC+CGG - Chr7:89024611-89024630 MsG0780041273.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr7 gene 89024468 89024893 89024468 ID=MsG0780041273.01;Name=MsG0780041273.01
Chr7 mRNA 89024468 89024893 89024468 ID=MsG0780041273.01.T01;Parent=MsG0780041273.01;Name=MsG0780041273.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|141
Chr7 exon 89024468 89024893 89024468 ID=MsG0780041273.01.T01:exon:3468;Parent=MsG0780041273.01.T01
Chr7 CDS 89024468 89024893 89024468 ID=MsG0780041273.01.T01:cds;Parent=MsG0780041273.01.T01
Gene Sequence

>MsG0780041273.01.T01

ATGAGTTCCAAAGGTGCCACAACTACAACAAAGGAGGAAGAGGCAAACCAAAAGCCTCCAAATCCGTTTCAAGATCTTCAAAAGCCGGTCTTCAATTCCCGTCGGAAGAATCGCAAGATTCTCAAGGCTGGAAAATACGCCGAGCGTGTTGGCGCCGGTGCTCCGGTCTATCTCTCTGCTGTTCTCGAGTATCTCGCTGCTGAGGTTTTGGAATTGGCTGGTAATGCTGCGAGGGACAACAAAAAGAATCGAATTGTTCCGAGACACATTCAGCTTGCTGTGAGGAATGATGAAGAACTCAGCAAGCTTTTGGGTGCCGTAACCATTGCTAATGGTGGTGTTTTGCCCAATATTCATCAGACATTGCTTCCTAAGAAGGTTGGAAAAGGGAAAGGTGAAATTGGATCTGCTTCTCAAGAATTTTAG

Protein sequence

>MsG0780041273.01.T01

MSSKGATTTTKEEEANQKPPNPFQDLQKPVFNSRRKNRKILKAGKYAERVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKNRIVPRHIQLAVRNDEELSKLLGAVTIANGGVLPNIHQTLLPKKVGKGKGEIGSASQEF*