AlfalfaGEDB | MsG0580025974.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025974.01

KEGG: K00799
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025974.01.T01	PNX70863.1	81.429	70	13	0	11	80	30	99	8.45E-32	117

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025974.01.T01	Q9FUS8	63.333	60	22	0	11	70	160	219	1.98E-18	79

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025974.01.T01	A0A2K3KX54	81.429	70	13	0	11	80	30	99	4.04e-32	117

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048357.01	MsG0580025974.01	0.848380	6.361121e-60	1.165100e-56
MsG0180000404.01	MsG0580025974.01	0.835633	1.498143e-56	1.842617e-53
MsG0180001200.01	MsG0580025974.01	0.801941	7.100836e-49	3.434453e-46
MsG0180001771.01	MsG0580025974.01	0.823577	1.285085e-53	1.110869e-50
MsG0180003741.01	MsG0580025974.01	0.851185	1.046184e-60	2.100362e-57
MsG0180005540.01	MsG0580025974.01	0.813359	2.664460e-51	1.736321e-48
MsG0380011496.01	MsG0580025974.01	0.815705	8.060622e-52	5.598441e-49
MsG0380011501.01	MsG0580025974.01	0.804190	2.433420e-49	1.246656e-46
MsG0380011675.01	MsG0580025974.01	0.814685	1.358351e-51	9.175297e-49
MsG0480018909.01	MsG0580025974.01	0.824645	7.214847e-54	6.429557e-51
MsG0480019279.01	MsG0580025974.01	0.827017	1.973004e-54	1.881418e-51
MsG0480024022.01	MsG0580025974.01	0.840804	6.975081e-58	1.004936e-54
MsG0580024966.01	MsG0580025974.01	0.823649	1.236513e-53	1.071130e-50
MsG0580025466.01	MsG0580025974.01	0.843334	1.493947e-58	2.330080e-55
MsG0580025973.01	MsG0580025974.01	0.842802	2.070252e-58	3.174577e-55
MsG0580025974.01	MsG0580026000.01	0.827411	1.588158e-54	1.532160e-51
MsG0580025974.01	MsG0580027925.01	0.816123	6.502494e-52	4.568092e-49
MsG0580025974.01	MsG0580029310.01	0.825396	4.795948e-54	4.366113e-51
MsG0580025974.01	MsG0580029417.01	0.813684	2.259647e-51	1.485553e-48
MsG0580025974.01	MsG0580029418.01	0.819022	1.444273e-52	1.098774e-49
MsG0580025974.01	MsG0680031707.01	0.808608	2.849215e-50	1.636265e-47
MsG0580025974.01	MsG0680031959.01	0.836195	1.078540e-56	1.349609e-53
MsG0580025974.01	MsG0680033143.01	0.802480	5.499564e-49	2.696808e-46
MsG0580025974.01	MsG0780040846.01	0.815098	1.100059e-51	7.513633e-49
MsG0580025974.01	MsG0780041259.01	0.857122	2.024273e-62	4.943269e-59
MsG0580025974.01	MsG0780041698.01	0.838184	3.343421e-57	4.443268e-54
MsG0580025974.01	MsG0880045585.01	0.861064	1.333800e-63	3.725359e-60
MsG0580025974.01	MsG0880045586.01	0.813692	2.250082e-51	1.479601e-48
MsG0380016465.01	MsG0580025974.01	0.823479	1.354904e-53	1.167964e-50
MsG0280007305.01	MsG0580025974.01	0.800284	1.549412e-48	7.186486e-46
MsG0280008390.01	MsG0580025974.01	0.821769	3.384721e-53	2.779551e-50
MsG0280008391.01	MsG0580025974.01	0.827024	1.966182e-54	1.875268e-51
MsG0280008392.01	MsG0580025974.01	0.808939	2.420234e-50	1.402106e-47
MsG0280008395.01	MsG0580025974.01	0.811442	6.986893e-51	4.325562e-48
MsG0280010320.01	MsG0580025974.01	0.836681	8.113954e-57	1.030013e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025974.01.T01	MTR_5g037380	78.947	57	12	0	13	69	163	219	1.87e-27	99.8
MsG0580025974.01.T01	MTR_8g061950	59.016	61	25	0	11	71	161	221	4.28e-18	75.5

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025974.01.T01	AT1G10370	63.333	60	22	11	70	160	219	2.02e-19	79.0
MsG0580025974.01.T01	AT1G10360	60.000	60	24	11	70	160	219	3.04e-18	75.9
MsG0580025974.01.T01	AT1G59700	50.000	56	28	13	68	164	219	2.93e-13	62.8
MsG0580025974.01.T01	AT1G59670	42.857	63	36	13	75	164	226	1.87e-12	60.5
MsG0580025974.01.T01	AT1G69930	48.276	58	30	13	70	168	225	2.44e-11	57.4

Find 17 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCACAAGTTTATCAGTTTC+TGG	0.176738	5:-28411527	None:intergenic
TTGGATTTATTGATTGCTTT+TGG	0.190966	5:+28411374	MsG0580025974.01.T01:CDS
AGACTCCAGCTTTGGTGAAA+TGG	0.311703	5:+28411462	MsG0580025974.01.T01:CDS
GACTCCAGCTTTGGTGAAAT+GGG	0.333466	5:+28411463	MsG0580025974.01.T01:CDS
GACCCTCAACCAACTCAAAT+AGG	0.392914	5:-28411399	None:intergenic
TGCTGCTGATCCTTCTGTGA+AGG	0.439808	5:+28411496	MsG0580025974.01.T01:CDS
GGTCCTATTTGAGTTGGTTG+AGG	0.441711	5:+28411396	MsG0580025974.01.T01:CDS
GTCCTATTTGAGTTGGTTGA+GGG	0.442650	5:+28411397	MsG0580025974.01.T01:CDS
TCAGCCCATTTCACCAAAGC+TGG	0.450590	5:-28411467	None:intergenic
TGAAACAAAGACTCCAGCTT+TGG	0.477233	5:+28411454	MsG0580025974.01.T01:CDS
GCTGCTGATCCTTCTGTGAA+GGG	0.482984	5:+28411497	MsG0580025974.01.T01:CDS
TAAACTTGTGGAGTTTGCTA+AGG	0.485321	5:+28411538	MsG0580025974.01.T01:CDS
TGAGAAAATGACAGGAAAGA+AGG	0.539427	5:+28411424	MsG0580025974.01.T01:CDS
AAACTTGTGGAGTTTGCTAA+GGG	0.551366	5:+28411539	MsG0580025974.01.T01:CDS
ACCAGAAACTGATAAACTTG+TGG	0.558982	5:+28411526	MsG0580025974.01.T01:CDS
AGGGTCACTGAGAAAATGAC+AGG	0.565602	5:+28411416	MsG0580025974.01.T01:CDS
GGTAGAACTCCCTTCACAGA+AGG	0.612679	5:-28411506	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGGATTTATTGATTGCTTTT+GGG	+	Chr5:28411375-28411394	MsG0580025974.01.T01:CDS	25.0%
!!!	TTGGATTTATTGATTGCTTT+TGG	+	Chr5:28411374-28411393	MsG0580025974.01.T01:CDS	25.0%
!!!	TTTTTTGGAGGAGAGAAAAT+TGG	+	Chr5:28411355-28411374	MsG0580025974.01.T01:CDS	30.0%
	ACCAGAAACTGATAAACTTG+TGG	+	Chr5:28411526-28411545	MsG0580025974.01.T01:CDS	35.0%
	TCCACAAGTTTATCAGTTTC+TGG	-	Chr5:28411530-28411549	None:intergenic	35.0%
	TGAGAAAATGACAGGAAAGA+AGG	+	Chr5:28411424-28411443	MsG0580025974.01.T01:CDS	35.0%
!!	AAACTTGTGGAGTTTGCTAA+GGG	+	Chr5:28411539-28411558	MsG0580025974.01.T01:CDS	35.0%
!!	TAAACTTGTGGAGTTTGCTA+AGG	+	Chr5:28411538-28411557	MsG0580025974.01.T01:CDS	35.0%
!!	TAAGGGTTTTGTGCAAAGAA+TGG	+	Chr5:28411556-28411575	MsG0580025974.01.T01:CDS	35.0%
	TGAAACAAAGACTCCAGCTT+TGG	+	Chr5:28411454-28411473	MsG0580025974.01.T01:CDS	40.0%
!!	CTTTTGGGTCCTATTTGAGT+TGG	+	Chr5:28411390-28411409	MsG0580025974.01.T01:CDS	40.0%
!!	GTCCTATTTGAGTTGGTTGA+GGG	+	Chr5:28411397-28411416	MsG0580025974.01.T01:CDS	40.0%
	AGGGTCACTGAGAAAATGAC+AGG	+	Chr5:28411416-28411435	MsG0580025974.01.T01:CDS	45.0%
	GACCCTCAACCAACTCAAAT+AGG	-	Chr5:28411402-28411421	None:intergenic	45.0%
!	AGACTCCAGCTTTGGTGAAA+TGG	+	Chr5:28411462-28411481	MsG0580025974.01.T01:CDS	45.0%
!	GACTCCAGCTTTGGTGAAAT+GGG	+	Chr5:28411463-28411482	MsG0580025974.01.T01:CDS	45.0%
!!	GGTCCTATTTGAGTTGGTTG+AGG	+	Chr5:28411396-28411415	MsG0580025974.01.T01:CDS	45.0%
	GCTGCTGATCCTTCTGTGAA+GGG	+	Chr5:28411497-28411516	MsG0580025974.01.T01:CDS	50.0%
	GGTAGAACTCCCTTCACAGA+AGG	-	Chr5:28411509-28411528	None:intergenic	50.0%
	TCAGCCCATTTCACCAAAGC+TGG	-	Chr5:28411470-28411489	None:intergenic	50.0%
	TGCTGCTGATCCTTCTGTGA+AGG	+	Chr5:28411496-28411515	MsG0580025974.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	28411350	28411601	28411350	ID=MsG0580025974.01;Name=MsG0580025974.01
Chr5	mRNA	28411350	28411601	28411350	ID=MsG0580025974.01.T01;Parent=MsG0580025974.01;Name=MsG0580025974.01.T01;_AED=0.46;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|83
Chr5	exon	28411350	28411601	28411350	ID=MsG0580025974.01.T01:exon:7487;Parent=MsG0580025974.01.T01
Chr5	CDS	28411350	28411601	28411350	ID=MsG0580025974.01.T01:cds;Parent=MsG0580025974.01.T01

Gene Sequence	>MsG0580025974.01.T01 ATGTCTTTTTTGGAGGAGAGAAAATTGGATTTATTGATTGCTTTTGGGTCCTATTTGAGTTGGTTGAGGGTCACTGAGAAAATGACAGGAAAGAAGGTGCTTGATGAAACAAAGACTCCAGCTTTGGTGAAATGGGCTGAAGCTTTTGCTGCTGATCCTTCTGTGAAGGGAGTTCTACCAGAAACTGATAAACTTGTGGAGTTTGCTAAGGGTTTTGTGCAAAGAATGGTTGCTTCTACTCTACTCCAATAA
Protein sequence	>MsG0580025974.01.T01 MSFLEERKLDLLIAFGSYLSWLRVTEKMTGKKVLDETKTPALVKWAEAFAADPSVKGVLPETDKLVEFAKGFVQRMVASTLLQ*