AlfalfaGEDB | MsG0180005540.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005540.01

KEGG: K20718
GO: -
PFAM: PF08263

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005540.01.T01	XP_013469721.1	60	110	20	2	3	109	2	90	1.89E-26	113

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005540.01.T01	Q6XAT2	56.164	73	28	2	39	109	8	78	9.09E-19	83.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048357.01	MsG0180005540.01	0.847303	1.259386e-59	2.228582e-56
MsG0180000632.01	MsG0180005540.01	0.820697	5.979970e-53	4.765672e-50
MsG0180001200.01	MsG0180005540.01	0.814985	1.165773e-51	7.938496e-49
MsG0180001771.01	MsG0180005540.01	0.803495	3.392924e-49	1.707554e-46
MsG0180003741.01	MsG0180005540.01	0.832636	8.442978e-56	9.492932e-53
MsG0180005113.01	MsG0180005540.01	0.807167	5.770644e-50	3.191536e-47
MsG0180005540.01	MsG0280007197.01	0.812588	3.932115e-51	2.510017e-48
MsG0180005540.01	MsG0280008390.01	0.816508	5.333429e-52	3.786290e-49
MsG0180005540.01	MsG0280008395.01	0.808656	2.782032e-50	1.599711e-47
MsG0180005540.01	MsG0280010163.01	0.834926	2.259415e-56	2.720885e-53
MsG0180005540.01	MsG0280010320.01	0.802448	5.583666e-49	2.735861e-46
MsG0180005540.01	MsG0380011496.01	0.812039	5.181616e-51	3.258974e-48
MsG0180005540.01	MsG0380011501.01	0.811855	5.682525e-51	3.556381e-48
MsG0180005540.01	MsG0380011675.01	0.846254	2.438150e-59	4.171397e-56
MsG0180005540.01	MsG0380016465.01	0.811414	7.084339e-51	4.382590e-48
MsG0180005540.01	MsG0480018909.01	0.821330	4.276532e-53	3.468827e-50
MsG0180005540.01	MsG0480024022.01	0.828865	7.090174e-55	7.134603e-52
MsG0180005540.01	MsG0580024966.01	0.819150	1.350715e-52	1.031079e-49
MsG0180005540.01	MsG0580025138.01	0.808650	2.790334e-50	1.604235e-47
MsG0180005540.01	MsG0580025466.01	0.803459	3.450884e-49	1.735093e-46
MsG0180005540.01	MsG0580025974.01	0.813359	2.664460e-51	1.736321e-48
MsG0180005540.01	MsG0580027925.01	0.801068	1.071881e-48	5.071563e-46
MsG0180005540.01	MsG0580029310.01	0.807247	5.548098e-50	3.074973e-47
MsG0180005540.01	MsG0580029406.01	0.810811	9.575166e-51	5.829476e-48
MsG0180005540.01	MsG0580029418.01	0.800951	1.132325e-48	5.341726e-46
MsG0180005540.01	MsG0680031707.01	0.829116	6.161818e-55	6.247052e-52
MsG0180005540.01	MsG0680031959.01	0.852730	3.810792e-61	8.038404e-58
MsG0180005540.01	MsG0680033143.01	0.816551	5.216031e-52	3.707329e-49
MsG0180005540.01	MsG0680035683.01	0.816312	5.900155e-52	4.166697e-49
MsG0180005540.01	MsG0780040286.01	0.815858	7.453818e-52	5.198281e-49
MsG0180005540.01	MsG0780040966.01	0.834146	3.548404e-56	4.173635e-53
MsG0180005540.01	MsG0780041259.01	0.828817	7.278968e-55	7.315020e-52
MsG0180005540.01	MsG0780041698.01	0.814128	1.804038e-51	1.200330e-48
MsG0180005540.01	MsG0880041834.01	0.802123	6.515042e-49	3.165772e-46
MsG0180005540.01	MsG0880041858.01	0.826058	3.340279e-54	3.098881e-51
MsG0180005540.01	MsG0880042959.01	0.801298	9.616613e-49	4.576463e-46
MsG0180005540.01	MsG0880045585.01	0.857657	1.406054e-62	3.496069e-59
MsG0180005540.01	MsG0880045586.01	0.839150	1.882324e-57	2.576382e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005540.01.T01	MTR_1g102500	60.000	110	20	2	3	109	2	90	2.29e-30	113

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005540.01.T01	AT5G07180	56.164	73	28	2	39	109	8	78	9.26e-20	83.2
MsG0180005540.01.T01	AT5G62230	64.151	53	17	1	57	109	26	76	8.58e-17	74.7
MsG0180005540.01.T01	AT5G62230	64.151	53	17	1	57	109	26	76	8.81e-17	74.7

Find 14 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCATTAGGACAAGCATTAA+TGG	0.273932	1:+93050555	MsG0180005540.01.T01:CDS
GAATGTTTGGTAAATGCATT+AGG	0.307125	1:+93050541	MsG0180005540.01.T01:CDS
TTTGTTACAATGAAAGAAAA+AGG	0.312795	1:+93048676	None:intergenic
GAAAACCTCCATTTGGTTGT+TGG	0.395503	1:-93048723	None:intergenic
AGAACATGAAAACCTCCATT+TGG	0.440529	1:-93048730	None:intergenic
TCGCTGAGTGAAAATGCTAA+TGG	0.472868	1:-93048775	None:intergenic
TTTCTGTGATAATGCCAGTC+AGG	0.492005	1:+93050666	MsG0180005540.01.T01:CDS
GGTTTGGTTCTATTGTTCAC+TGG	0.496589	1:+93048700	MsG0180005540.01.T01:CDS
TTCACTGGCCAACAACCAAA+TGG	0.496754	1:+93048715	MsG0180005540.01.T01:CDS
TAGCTGACGTTCTCCTTGAC+TGG	0.510030	1:+93050602	MsG0180005540.01.T01:CDS
AACGTCAGCTATGTTGTTGA+AGG	0.525568	1:-93050590	None:intergenic
TACAACAGTAAGAGCCTGAC+TGG	0.557559	1:-93050680	None:intergenic
TGTGTACATCATTCCAGTCA+AGG	0.594262	1:-93050615	None:intergenic
ACTGGCCAACAACCAAATGG+AGG	0.676475	1:+93048718	MsG0180005540.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAATAAAAATAAAAAAAA+AGG	-	Chr1:93049181-93049200	None:intergenic	0.0%
!!	AATTAAGAAAAAAATAATTG+TGG	+	Chr1:93049934-93049953	MsG0180005540.01.T01:intron	10.0%
!!	ATATAAATAGTGTAATTTAA+AGG	+	Chr1:93050243-93050262	MsG0180005540.01.T01:intron	10.0%
!!	AAATAAAAGAACAAGTAAAA+AGG	-	Chr1:93050420-93050439	None:intergenic	15.0%
!!!	ATTGTAAAATTGATTTTGAA+TGG	+	Chr1:93049430-93049449	MsG0180005540.01.T01:intron	15.0%
!!	ACTAATAAGTTAACCAAATA+TGG	-	Chr1:93048895-93048914	None:intergenic	20.0%
!!	CTTAGAGAAATTTGTAATTT+AGG	+	Chr1:93050085-93050104	MsG0180005540.01.T01:intron	20.0%
!!!	AGATCATTTTTATTTTCTGT+TGG	+	Chr1:93049135-93049154	MsG0180005540.01.T01:intron	20.0%
!!!	TGAAATTTTTTAAGTGTGTT+TGG	+	Chr1:93049392-93049411	MsG0180005540.01.T01:intron	20.0%
!!!	TTATTTTTATTATTGTGTGC+TGG	+	Chr1:93049187-93049206	MsG0180005540.01.T01:intron	20.0%
!	AAATAAAATCGTAAAGCACA+TGG	-	Chr1:93049254-93049273	None:intergenic	25.0%
!	ACCTCACATTGATAAAATAT+TGG	-	Chr1:93050288-93050307	None:intergenic	25.0%
!	CCTAATAATATCAACATCAT+CGG	+	Chr1:93050032-93050051	MsG0180005540.01.T01:intron	25.0%
!	GAAATAAAGACATTGAACTT+TGG	-	Chr1:93049048-93049067	None:intergenic	25.0%
!	GCAATGTGTGTATAATTTAT+TGG	+	Chr1:93050187-93050206	MsG0180005540.01.T01:intron	25.0%
!	TAAAACCATACACATATGTT+TGG	+	Chr1:93050472-93050491	MsG0180005540.01.T01:intron	25.0%
!	TGATGTTGATATTATTAGGT+CGG	-	Chr1:93050031-93050050	None:intergenic	25.0%
!!	CTTCTTTTTCTTTCCATATT+TGG	+	Chr1:93048879-93048898	MsG0180005540.01.T01:intron	25.0%
!!	TAACTTAACTGTTTTCCTTT+TGG	+	Chr1:93048966-93048985	MsG0180005540.01.T01:intron	25.0%
!!	TCCAATATTTTATCAATGTG+AGG	+	Chr1:93050284-93050303	MsG0180005540.01.T01:intron	25.0%
!!	TTTTATGTGTAAAGACAAGA+GGG	-	Chr1:93048848-93048867	None:intergenic	25.0%
!!	TTTTTATGTGTAAAGACAAG+AGG	-	Chr1:93048849-93048868	None:intergenic	25.0%
!!!	AAACTTCCTATTTTTTTCCA+TGG	+	Chr1:93050350-93050369	MsG0180005540.01.T01:intron	25.0%
!!!	GCATTTAAGAAGTGTTTAAT+TGG	+	Chr1:93049802-93049821	MsG0180005540.01.T01:intron	25.0%
!!!	GTGTCTTATTTTTGAATGTT+TGG	+	Chr1:93050528-93050547	MsG0180005540.01.T01:CDS	25.0%
!!!	TTTTTTAAGTGTGTTTGGTT+AGG	+	Chr1:93049397-93049416	MsG0180005540.01.T01:intron	25.0%
	AGCATCAAGTATAAAGATTC+AGG	-	Chr1:93049364-93049383	None:intergenic	30.0%
	CAATTTCTACGTCTTAAAGT+TGG	-	Chr1:93049519-93049538	None:intergenic	30.0%
	CCGATGATGTTGATATTATT+AGG	-	Chr1:93050035-93050054	None:intergenic	30.0%
	GAATGTTTGGTAAATGCATT+AGG	+	Chr1:93050541-93050560	MsG0180005540.01.T01:CDS	30.0%
	GACACTTCATAAACTTAATC+TGG	-	Chr1:93050513-93050532	None:intergenic	30.0%
	TCAAAAGCTAGTCATAAAAG+AGG	-	Chr1:93049624-93049643	None:intergenic	30.0%
	TCATAAAAGAGGCAAACATT+GGG	-	Chr1:93049613-93049632	None:intergenic	30.0%
	TTTATGTGTAAAGACAAGAG+GGG	-	Chr1:93048847-93048866	None:intergenic	30.0%
!	AATTTTCCAAACATGCTAAG+TGG	+	Chr1:93049654-93049673	MsG0180005540.01.T01:intron	30.0%
!	CTTTTCCAAACATATGTGTA+TGG	-	Chr1:93050480-93050499	None:intergenic	30.0%
!	TTCGACTAGTAATGTTTTGT+CGG	+	Chr1:93049101-93049120	MsG0180005540.01.T01:intron	30.0%
!!	CAAAATGCTACACTTTTTGT+TGG	+	Chr1:93048918-93048937	MsG0180005540.01.T01:intron	30.0%
!!!	TTGTTGGTTTTTCACAAGAT+TGG	+	Chr1:93048934-93048953	MsG0180005540.01.T01:intron	30.0%
	AATGAAAGTGAACCACCAAA+AGG	-	Chr1:93048984-93049003	None:intergenic	35.0%
	AGAACATGAAAACCTCCATT+TGG	-	Chr1:93048733-93048752	None:intergenic	35.0%
	GCACATCCATGGAAAAAAAT+AGG	-	Chr1:93050359-93050378	None:intergenic	35.0%
	GCATGTTTGGAAAATTGTTG+AGG	-	Chr1:93049650-93049669	None:intergenic	35.0%
	GGCTTATTTATTTCCACTCA+CGG	-	Chr1:93049233-93049252	None:intergenic	35.0%
	GTCATAAAAGAGGCAAACAT+TGG	-	Chr1:93049614-93049633	None:intergenic	35.0%
	TGCATTAGGACAAGCATTAA+TGG	+	Chr1:93050555-93050574	MsG0180005540.01.T01:CDS	35.0%
	TGGATTTGATGAAGCATGTT+TGG	+	Chr1:93049155-93049174	MsG0180005540.01.T01:intron	35.0%
!	ACAATGATGACTTTTGCTCA+TGG	+	Chr1:93050635-93050654	MsG0180005540.01.T01:CDS	35.0%
!	CTTAACTGTTTTCCTTTTGG+TGG	+	Chr1:93048969-93048988	MsG0180005540.01.T01:intron	35.0%
!!!	GTACCAATGTTGTTTTGTAG+TGG	+	Chr1:93049721-93049740	MsG0180005540.01.T01:intron	35.0%
!!!	GTCAATCATTTTTTCCCTGA+TGG	+	Chr1:93050442-93050461	MsG0180005540.01.T01:intron	35.0%
	AACGTCAGCTATGTTGTTGA+AGG	-	Chr1:93050593-93050612	None:intergenic	40.0%
	CTTAACCATAGAGCTTCAAG+AGG	-	Chr1:93049334-93049353	None:intergenic	40.0%
	GAAAACCTCCATTTGGTTGT+TGG	-	Chr1:93048726-93048745	None:intergenic	40.0%
	GAGCCACTACAAAACAACAT+TGG	-	Chr1:93049727-93049746	None:intergenic	40.0%
	GTCACTATGCTTGTGCAAAT+TGG	-	Chr1:93050325-93050344	None:intergenic	40.0%
	TCGCTGAGTGAAAATGCTAA+TGG	-	Chr1:93048778-93048797	None:intergenic	40.0%
	TCTTTCCTCTTGAAGCTCTA+TGG	+	Chr1:93049326-93049345	MsG0180005540.01.T01:intron	40.0%
	TGTGTACATCATTCCAGTCA+AGG	-	Chr1:93050618-93050637	None:intergenic	40.0%
	TTTCCATGGATGTGCATGTA+TGG	+	Chr1:93050364-93050383	MsG0180005540.01.T01:intron	40.0%
!	AACGCAGGATTTTATGGTTG+CGG	-	Chr1:93049986-93050005	None:intergenic	40.0%
!	AATGAGTGATTTTCCGTGAG+TGG	+	Chr1:93049217-93049236	MsG0180005540.01.T01:intron	40.0%
!	AGTTCGAACGCAGGATTTTA+TGG	-	Chr1:93049992-93050011	None:intergenic	40.0%
!	GACTAGTAATGTTTTGTCGG+CGG	+	Chr1:93049104-93049123	MsG0180005540.01.T01:intron	40.0%
!	TTTCTGTGATAATGCCAGTC+AGG	+	Chr1:93050666-93050685	MsG0180005540.01.T01:CDS	40.0%
!!	CAGTTGTTTTCTATGAGCAG+CGG	+	Chr1:93049750-93049769	MsG0180005540.01.T01:intron	40.0%
!!	GGTTTGGTTCTATTGTTCAC+TGG	+	Chr1:93048700-93048719	MsG0180005540.01.T01:CDS	40.0%
!!!	GGTTTTAAACTGCACCATCA+GGG	-	Chr1:93050459-93050478	None:intergenic	40.0%
!!!	TGGTTTTAAACTGCACCATC+AGG	-	Chr1:93050460-93050479	None:intergenic	40.0%
	AAACCCAAGATGCTACCAGA+GGG	+	Chr1:93049484-93049503	MsG0180005540.01.T01:intron	45.0%
	ACTCCATACATGCACATCCA+TGG	-	Chr1:93050370-93050389	None:intergenic	45.0%
	ATGCTAAGTGGCGAATGTAG+TGG	+	Chr1:93049666-93049685	MsG0180005540.01.T01:intron	45.0%
	CATCAACATAGTTCGAACGC+AGG	-	Chr1:93050001-93050020	None:intergenic	45.0%
	CATTCGCCACTTAGCATGTT+TGG	-	Chr1:93049663-93049682	None:intergenic	45.0%
	TAAACCCAAGATGCTACCAG+AGG	+	Chr1:93049483-93049502	MsG0180005540.01.T01:intron	45.0%
	TACAACAGTAAGAGCCTGAC+TGG	-	Chr1:93050683-93050702	None:intergenic	45.0%
	TTCACTGGCCAACAACCAAA+TGG	+	Chr1:93048715-93048734	MsG0180005540.01.T01:CDS	45.0%
!	CAGGATTTTATGGTTGCGGT+GGG	-	Chr1:93049982-93050001	None:intergenic	45.0%
!!	AGTTGGTAGAATCTTCCCTC+TGG	-	Chr1:93049502-93049521	None:intergenic	45.0%
!!	GGTTCGAACGGTTGTTTTCT+AGG	+	Chr1:93049687-93049706	MsG0180005540.01.T01:intron	45.0%
!!!	TGGTTGCGGTGGGTTATTTT+AGG	-	Chr1:93049972-93049991	None:intergenic	45.0%
	ACTGGCCAACAACCAAATGG+AGG	+	Chr1:93048718-93048737	MsG0180005540.01.T01:CDS	50.0%
	CGACTGGGTGTCAAATCAAG+AGG	-	Chr1:93050134-93050153	None:intergenic	50.0%
	CTTCCCTCTGGTAGCATCTT+GGG	-	Chr1:93049490-93049509	None:intergenic	50.0%
	GGCGAATGTAGTGGTTCGAA+CGG	+	Chr1:93049675-93049694	MsG0180005540.01.T01:intron	50.0%
	TAGCTGACGTTCTCCTTGAC+TGG	+	Chr1:93050602-93050621	MsG0180005540.01.T01:CDS	50.0%
	TCTGAACCTGAAGAGCGACT+GGG	-	Chr1:93050149-93050168	None:intergenic	50.0%
	TCTTCCCTCTGGTAGCATCT+TGG	-	Chr1:93049491-93049510	None:intergenic	50.0%
	TTCTGAACCTGAAGAGCGAC+TGG	-	Chr1:93050150-93050169	None:intergenic	50.0%
!	GATGACTTTTGCTCATGGCG+TGG	+	Chr1:93050640-93050659	MsG0180005540.01.T01:CDS	50.0%
!	GCAGGATTTTATGGTTGCGG+TGG	-	Chr1:93049983-93050002	None:intergenic	50.0%
!	GCATTTTCACTCAGCGACGA+AGG	+	Chr1:93048781-93048800	MsG0180005540.01.T01:CDS	50.0%
	TTGACACCCAGTCGCTCTTC+AGG	+	Chr1:93050140-93050159	MsG0180005540.01.T01:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	93048685	93050711	93048685	ID=MsG0180005540.01;Name=MsG0180005540.01
Chr1	mRNA	93048685	93050711	93048685	ID=MsG0180005540.01.T01;Parent=MsG0180005540.01;Name=MsG0180005540.01.T01;_AED=0.52;_eAED=0.52;_QI=0\|0\|0\|1\|0\|0\|2\|0\|109
Chr1	exon	93048685	93048816	93048685	ID=MsG0180005540.01.T01:exon:10016;Parent=MsG0180005540.01.T01
Chr1	exon	93050514	93050711	93050514	ID=MsG0180005540.01.T01:exon:10017;Parent=MsG0180005540.01.T01
Chr1	CDS	93048685	93048816	93048685	ID=MsG0180005540.01.T01:cds;Parent=MsG0180005540.01.T01
Chr1	CDS	93050514	93050711	93050514	ID=MsG0180005540.01.T01:cds;Parent=MsG0180005540.01.T01

Gene Sequence	>MsG0180005540.01.T01 ATGAAAGAAAAAGGAGGTTTGGTTCTATTGTTCACTGGCCAACAACCAAATGGAGGTTTTCATGTTCTCATCACGTTGCTTCTTCTTTCTCCATTAGCATTTTCACTCAGCGACGAAGGTTTGTGTTTTTGTATTAAGTTTATGAAGTGTCTTATTTTTGAATGTTTGGTAAATGCATTAGGACAAGCATTAATGGCAATGAAGTCTTCCTTCAACAACATAGCTGACGTTCTCCTTGACTGGAATGATGTACACAATGATGACTTTTGCTCATGGCGTGGAGTTTTCTGTGATAATGCCAGTCAGGCTCTTACTGTTGTATCTCTGTGA
Protein sequence	>MsG0180005540.01.T01 MKEKGGLVLLFTGQQPNGGFHVLITLLLLSPLAFSLSDEGLCFCIKFMKCLIFECLVNALGQALMAMKSSFNNIADVLLDWNDVHNDDFCSWRGVFCDNASQALTVVSL*