AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033617.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033617.01.T01 MTR_0020s0120 98.837 172 2 0 1 172 1 172 2.01e-125 350
MsG0680033617.01.T01 MTR_0020s0120 94.767 172 2 1 1 172 1 165 4.74e-117 328
MsG0680033617.01.T01 MTR_7g104460 70.833 168 48 1 6 172 7 174 1.04e-87 254
MsG0680033617.01.T01 MTR_2g086270 66.474 173 57 1 1 172 1 173 1.48e-86 251
MsG0680033617.01.T01 MTR_1g060190 62.264 159 58 1 6 162 7 165 6.27e-75 222
MsG0680033617.01.T01 MTR_7g084970 59.036 166 68 0 7 172 8 173 1.40e-73 219
MsG0680033617.01.T01 MTR_6g033040 58.084 167 70 0 6 172 7 173 3.30e-69 207
MsG0680033617.01.T01 MTR_7g006690 57.485 167 70 1 7 172 8 174 4.11e-67 202
MsG0680033617.01.T01 MTR_7g085020 57.895 171 71 1 2 172 5 174 4.69e-67 202
MsG0680033617.01.T01 MTR_7g006630 56.213 169 73 1 5 172 1 169 2.01e-66 201
MsG0680033617.01.T01 MTR_8g106840 53.757 173 78 2 1 172 2 173 2.40e-63 192
MsG0680033617.01.T01 MTR_7g085040 53.750 160 73 1 13 172 11 169 2.84e-59 182
MsG0680033617.01.T01 MTR_5g078320 46.575 73 38 1 100 171 16 88 1.13e-16 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033617.01.T01 AT5G62040 68.391 174 53 2 1 172 1 174 1.95e-84 246
MsG0680033617.01.T01 AT2G27550 69.822 169 49 2 6 172 7 175 7.58e-83 242
MsG0680033617.01.T01 AT5G03840 62.791 172 63 1 2 172 6 177 2.94e-81 238
MsG0680033617.01.T01 AT1G65480 62.275 167 63 0 6 172 7 173 8.11e-76 224
MsG0680033617.01.T01 AT1G65480 62.275 167 63 0 6 172 51 217 1.37e-75 225
MsG0680033617.01.T01 AT4G20370 58.683 167 69 0 6 172 7 173 9.27e-69 206
MsG0680033617.01.T01 AT1G18100 52.299 174 80 2 1 172 1 173 2.86e-61 187

Find 43 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCTAGTGATCCACATTTA+AGG 0.192828 6:+69573045 MsG0680033617.01.T01:CDS
TGGATTCCAATCACTGGCTT+TGG 0.338572 6:-69573635 None:intergenic
GGTACCACAGATGTCTCTTT+TGG 0.356770 6:+69573198 MsG0680033617.01.T01:CDS
TCACTAGGACTAGGAGCATC+TGG 0.365823 6:-69573032 None:intergenic
GGCTTGTTCATAACAATAGA+AGG 0.369931 6:-69572437 None:intergenic
CATGAGATCTGCTTATACTT+TGG 0.379247 6:+69572484 MsG0680033617.01.T01:CDS
ACAAGATGAAGACATATCTA+TGG 0.389834 6:-69573655 None:intergenic
AACAAGCCAAGGGTTGATAT+TGG 0.415081 6:+69572452 MsG0680033617.01.T01:CDS
TCCTAGTGATCCACATTTAA+GGG 0.415346 6:+69573046 MsG0680033617.01.T01:CDS
GAGAATCCAAAGCCAGTGAT+TGG 0.439509 6:+69573629 MsG0680033617.01.T01:CDS
ATTTACAATGTCTAGGCCAT+TGG 0.453195 6:+69572301 None:intergenic
TTGAAGTAAACTGCAGCAAC+AGG 0.458327 6:-69573770 None:intergenic
ATGACCATTTGCAACTTGCT+TGG 0.464272 6:-69572405 None:intergenic
CTTTCTGTAGGAAGAGTGAT+AGG 0.465009 6:+69572329 MsG0680033617.01.T01:CDS
GTCACATTCATTCTTACACT+TGG 0.469107 6:-69572374 None:intergenic
GAGAAACTGCAGCAAGAAGA+AGG 0.491502 6:+69573801 MsG0680033617.01.T01:CDS
ATCACTCTTCCTACAGAAAG+TGG 0.502508 6:-69572326 None:intergenic
TTGAAATTGTCTCTAGAAGA+TGG 0.510509 6:-69573710 None:intergenic
TCAACCAAGCAAGTTGCAAA+TGG 0.516791 6:+69572401 MsG0680033617.01.T01:CDS
TTCTCACAGGAGAATAACCT+TGG 0.519597 6:+69573743 MsG0680033617.01.T01:CDS
CTATTGTTATGAACAAGCCA+AGG 0.525548 6:+69572441 MsG0680033617.01.T01:CDS
CATTGTGATTTACAATGTCT+AGG 0.527247 6:+69572294 None:intergenic
TCATCTTGTTCAAGCAGAGA+AGG 0.540166 6:+69573669 MsG0680033617.01.T01:CDS
TGAAATTGTCTCTAGAAGAT+GGG 0.541509 6:-69573709 None:intergenic
AAATGTGGATCACTAGGACT+AGG 0.546629 6:-69573041 None:intergenic
CACTAGGACTAGGAGCATCT+GGG 0.549402 6:-69573031 None:intergenic
AGGATGGTTACAGATATTCC+AGG 0.555677 6:+69573177 MsG0680033617.01.T01:intron
AAGCCAAGGGTTGATATTGG+TGG 0.558895 6:+69572455 MsG0680033617.01.T01:CDS
AAAGAGACATCTGTGGTACC+TGG 0.563172 6:-69573195 None:intergenic
CAACACAAGGAGATTCTCAC+AGG 0.564893 6:+69573730 MsG0680033617.01.T01:CDS
TATCTATGGATTCCAATCAC+TGG 0.569156 6:-69573641 None:intergenic
ATTTAAGGGAACATCTCCAC+TGG 0.572031 6:+69573060 MsG0680033617.01.T01:CDS
TCACCACCAATATCAACCCT+TGG 0.583858 6:-69572458 None:intergenic
CCATTGGAACCACTTTCTGT+AGG 0.584120 6:+69572317 MsG0680033617.01.T01:CDS
TATTGTTATGAACAAGCCAA+GGG 0.586275 6:+69572442 MsG0680033617.01.T01:CDS
CTAGAGACAATTTCAACACA+AGG 0.589107 6:+69573717 MsG0680033617.01.T01:CDS
TCCCTTAAATGTGGATCACT+AGG 0.597152 6:-69573047 None:intergenic
CCTACAGAAAGTGGTTCCAA+TGG 0.603095 6:-69572317 None:intergenic
TGGAGATGTTCCCTTAAATG+TGG 0.614063 6:-69573056 None:intergenic
GAAATTGTCTCTAGAAGATG+GGG 0.619592 6:-69573708 None:intergenic
TGATGCAGGAAATGAGATTG+TGG 0.645531 6:+69573601 MsG0680033617.01.T01:intron
CTGCAGCAACAGGTAAACCA+AGG 0.708296 6:-69573760 None:intergenic
CTAACCAAAAGAGACATCTG+TGG 0.754543 6:-69573202 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAGAGAATAATTAATTA+AGG - Chr6:69573257-69573276 None:intergenic 10.0%
!! GTAAAAAAAATAATAACAAA+AGG + Chr6:69572753-69572772 MsG0680033617.01.T01:intron 10.0%
!!! ATATGTTTTTATATATTGAT+AGG + Chr6:69572974-69572993 MsG0680033617.01.T01:intron 10.0%
!! ATGTAACTTAATTAAAAGAA+AGG + Chr6:69573540-69573559 MsG0680033617.01.T01:intron 15.0%
!! GGAAATAAATATACTAATAA+AGG - Chr6:69572627-69572646 None:intergenic 15.0%
!! TTCATATGTTTCAAAATATA+AGG + Chr6:69572522-69572541 MsG0680033617.01.T01:intron 15.0%
!!! ATAACAAATGTTTTAGAAAT+CGG - Chr6:69572917-69572936 None:intergenic 15.0%
!!! TATTTAGTTTTTTTACTTGA+AGG - Chr6:69572708-69572727 None:intergenic 15.0%
!! CAATGATAAAAAACATGATT+TGG - Chr6:69572648-69572667 None:intergenic 20.0%
!! TAATTTATTACATATGTGCA+TGG + Chr6:69573389-69573408 MsG0680033617.01.T01:intron 20.0%
!!! TCTCTATATTTGTTAATCTA+AGG + Chr6:69573268-69573287 MsG0680033617.01.T01:intron 20.0%
!!! TGAAAATTTTAAAATCCTCA+TGG - Chr6:69573369-69573388 None:intergenic 20.0%
!!! TTCTAAAACATTTGTTATAG+TGG + Chr6:69572919-69572938 MsG0680033617.01.T01:intron 20.0%
! ACTAATAAAGGTAGTTAGTT+AGG - Chr6:69572615-69572634 None:intergenic 25.0%
! AGTTAGTTCAAACAATTAAG+CGG - Chr6:69572886-69572905 None:intergenic 25.0%
! ATTAAGGTTATATTGATCCT+AGG - Chr6:69573241-69573260 None:intergenic 25.0%
! TTAAACAACTTGAGTTCAAT+TGG + Chr6:69573335-69573354 MsG0680033617.01.T01:intron 25.0%
! TTACATGTATGGTTGTAAAA+AGG - Chr6:69573505-69573524 None:intergenic 25.0%
!!! AGTTTTATTATACAAACCAG+TGG - Chr6:69573079-69573098 None:intergenic 25.0%
AATCCAGTTCAATGAGAATA+AGG + Chr6:69572820-69572839 MsG0680033617.01.T01:intron 30.0%
ACAAGATGAAGACATATCTA+TGG - Chr6:69573658-69573677 None:intergenic 30.0%
ATACCTTATTCTCATTGAAC+TGG - Chr6:69572826-69572845 None:intergenic 30.0%
ATTGTTTGAACTAACTGGTT+CGG + Chr6:69572890-69572909 MsG0680033617.01.T01:intron 30.0%
GCTTAATTGTTTGAACTAAC+TGG + Chr6:69572885-69572904 MsG0680033617.01.T01:intron 30.0%
GTTAGTTCAAACAATTAAGC+GGG - Chr6:69572885-69572904 None:intergenic 30.0%
TACTTCGCTAATGTAACATA+TGG - Chr6:69572566-69572585 None:intergenic 30.0%
TATTGTTATGAACAAGCCAA+GGG + Chr6:69572442-69572461 MsG0680033617.01.T01:CDS 30.0%
TGTGATGCAATTTACATGTA+TGG - Chr6:69573516-69573535 None:intergenic 30.0%
TTAAATCCTCAAGTTCTTAG+TGG - Chr6:69573319-69573338 None:intergenic 30.0%
TTTATTCCCTCTTATCAACT+TGG + Chr6:69573139-69573158 MsG0680033617.01.T01:intron 30.0%
! TACATTAGCGAAGTATCTAT+CGG + Chr6:69572571-69572590 MsG0680033617.01.T01:intron 30.0%
! TGAAATTGTCTCTAGAAGAT+GGG - Chr6:69573712-69573731 None:intergenic 30.0%
! TTGAAATTGTCTCTAGAAGA+TGG - Chr6:69573713-69573732 None:intergenic 30.0%
!! ATAACACCAAGTTGATAAGA+GGG - Chr6:69573148-69573167 None:intergenic 30.0%
AAGGGAATTAACTTTGATGC+AGG + Chr6:69573587-69573606 MsG0680033617.01.T01:intron 35.0%
ATCGTAATCAAATCAAACCG+AGG + Chr6:69572847-69572866 MsG0680033617.01.T01:intron 35.0%
CAATTGGAAAATCAACCATG+AGG + Chr6:69573351-69573370 MsG0680033617.01.T01:intron 35.0%
CATGAGATCTGCTTATACTT+TGG + Chr6:69572484-69572503 MsG0680033617.01.T01:CDS 35.0%
CTAGAGACAATTTCAACACA+AGG + Chr6:69573717-69573736 MsG0680033617.01.T01:CDS 35.0%
CTATTGTTATGAACAAGCCA+AGG + Chr6:69572441-69572460 MsG0680033617.01.T01:CDS 35.0%
GTCACATTCATTCTTACACT+TGG - Chr6:69572377-69572396 None:intergenic 35.0%
TATCTATGGATTCCAATCAC+TGG - Chr6:69573644-69573663 None:intergenic 35.0%
TCATTGAACTGGATTTAACC+AGG - Chr6:69572815-69572834 None:intergenic 35.0%
TCCTAGTGATCCACATTTAA+GGG + Chr6:69573046-69573065 MsG0680033617.01.T01:CDS 35.0%
! CTTTTACCACTAAGAACTTG+AGG + Chr6:69573310-69573329 MsG0680033617.01.T01:intron 35.0%
! GAAATTGTCTCTAGAAGATG+GGG - Chr6:69573711-69573730 None:intergenic 35.0%
! GGCTTGTTCATAACAATAGA+AGG - Chr6:69572440-69572459 None:intergenic 35.0%
!! ATCTATCGGCTTTGATGATT+TGG + Chr6:69572585-69572604 MsG0680033617.01.T01:intron 35.0%
!! GATAACACCAAGTTGATAAG+AGG - Chr6:69573149-69573168 None:intergenic 35.0%
AAATGTGGATCACTAGGACT+AGG - Chr6:69573044-69573063 None:intergenic 40.0%
AGGATGGTTACAGATATTCC+AGG + Chr6:69573177-69573196 MsG0680033617.01.T01:intron 40.0%
ATCACTCTTCCTACAGAAAG+TGG - Chr6:69572329-69572348 None:intergenic 40.0%
ATTGATCCTAGGATAGAGAG+AGG - Chr6:69573230-69573249 None:intergenic 40.0%
ATTTAAGGGAACATCTCCAC+TGG + Chr6:69573060-69573079 MsG0680033617.01.T01:CDS 40.0%
CAAAAGGCAAAGATGAGAAC+CGG + Chr6:69572769-69572788 MsG0680033617.01.T01:intron 40.0%
CTAACCAAAAGAGACATCTG+TGG - Chr6:69573205-69573224 None:intergenic 40.0%
CTTTCTGTAGGAAGAGTGAT+AGG + Chr6:69572329-69572348 MsG0680033617.01.T01:CDS 40.0%
GTCCTAGTGATCCACATTTA+AGG + Chr6:69573045-69573064 MsG0680033617.01.T01:CDS 40.0%
GTGTTATCAATTTGCCAGGA+TGG + Chr6:69573161-69573180 MsG0680033617.01.T01:intron 40.0%
TCAACCAAGCAAGTTGCAAA+TGG + Chr6:69572401-69572420 MsG0680033617.01.T01:CDS 40.0%
TCATCTTGTTCAAGCAGAGA+AGG + Chr6:69573669-69573688 MsG0680033617.01.T01:CDS 40.0%
TCCCTTAAATGTGGATCACT+AGG - Chr6:69573050-69573069 None:intergenic 40.0%
TGATGCAGGAAATGAGATTG+TGG + Chr6:69573601-69573620 MsG0680033617.01.T01:intron 40.0%
TGGAATATCTGTAACCATCC+TGG - Chr6:69573178-69573197 None:intergenic 40.0%
TGGAGATGTTCCCTTAAATG+TGG - Chr6:69573059-69573078 None:intergenic 40.0%
TTCTCACAGGAGAATAACCT+TGG + Chr6:69573743-69573762 MsG0680033617.01.T01:CDS 40.0%
TTGAAGTAAACTGCAGCAAC+AGG - Chr6:69573773-69573792 None:intergenic 40.0%
TTGATCCTAGGATAGAGAGA+GGG - Chr6:69573229-69573248 None:intergenic 40.0%
! ATGACCATTTGCAACTTGCT+TGG - Chr6:69572408-69572427 None:intergenic 40.0%
!! AACAAGCCAAGGGTTGATAT+TGG + Chr6:69572452-69572471 MsG0680033617.01.T01:CDS 40.0%
!! CTTGGTGTTATCAATTTGCC+AGG + Chr6:69573157-69573176 MsG0680033617.01.T01:intron 40.0%
AAAGAGACATCTGTGGTACC+TGG - Chr6:69573198-69573217 None:intergenic 45.0%
CAACACAAGGAGATTCTCAC+AGG + Chr6:69573730-69573749 MsG0680033617.01.T01:CDS 45.0%
CCATTGGAACCACTTTCTGT+AGG + Chr6:69572317-69572336 MsG0680033617.01.T01:CDS 45.0%
CCTACAGAAAGTGGTTCCAA+TGG - Chr6:69572320-69572339 None:intergenic 45.0%
GAGAAACTGCAGCAAGAAGA+AGG + Chr6:69573801-69573820 MsG0680033617.01.T01:CDS 45.0%
GAGAATCCAAAGCCAGTGAT+TGG + Chr6:69573629-69573648 MsG0680033617.01.T01:CDS 45.0%
TAACCAGGTTAAACCGCAGT+TGG - Chr6:69572800-69572819 None:intergenic 45.0%
TCACCACCAATATCAACCCT+TGG - Chr6:69572461-69572480 None:intergenic 45.0%
TTAGTCCCTCTCTCTATCCT+AGG + Chr6:69573221-69573240 MsG0680033617.01.T01:intron 45.0%
! GGTACCACAGATGTCTCTTT+TGG + Chr6:69573198-69573217 MsG0680033617.01.T01:CDS 45.0%
! TAAACCGCAGTTGGTTCAAC+CGG - Chr6:69572791-69572810 None:intergenic 45.0%
!! AAGCCAAGGGTTGATATTGG+TGG + Chr6:69572455-69572474 MsG0680033617.01.T01:CDS 45.0%
!! TGGATTCCAATCACTGGCTT+TGG - Chr6:69573638-69573657 None:intergenic 45.0%
!!! ATAATTTAATTTTTTAACAA+AGG + Chr6:69573568-69573587 MsG0680033617.01.T01:intron 5.0%
!!! TAATTTAATTTTTTAACAAA+GGG + Chr6:69573569-69573588 MsG0680033617.01.T01:intron 5.0%
AGAACCGGTTGAACCAACTG+CGG + Chr6:69572784-69572803 MsG0680033617.01.T01:intron 50.0%
CTGCAGCAACAGGTAAACCA+AGG - Chr6:69573763-69573782 None:intergenic 50.0%
GAACCAACTGCGGTTTAACC+TGG + Chr6:69572794-69572813 MsG0680033617.01.T01:intron 50.0%
!! CACTAGGACTAGGAGCATCT+GGG - Chr6:69573034-69573053 None:intergenic 50.0%
!! TCACTAGGACTAGGAGCATC+TGG - Chr6:69573035-69573054 None:intergenic 50.0%
GCGGGAAGCAGTAAGTTCCT+CGG - Chr6:69572867-69572886 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 69572308 69573826 69572308 ID=MsG0680033617.01;Name=MsG0680033617.01
Chr6 mRNA 69572308 69573826 69572308 ID=MsG0680033617.01.T01;Parent=MsG0680033617.01;Name=MsG0680033617.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|4|0|172
Chr6 exon 69572308 69572505 69572308 ID=MsG0680033617.01.T01:exon:2250;Parent=MsG0680033617.01.T01
Chr6 exon 69573020 69573081 69573020 ID=MsG0680033617.01.T01:exon:2249;Parent=MsG0680033617.01.T01
Chr6 exon 69573179 69573219 69573179 ID=MsG0680033617.01.T01:exon:2248;Parent=MsG0680033617.01.T01
Chr6 exon 69573609 69573826 69573609 ID=MsG0680033617.01.T01:exon:2247;Parent=MsG0680033617.01.T01
Chr6 CDS 69572308 69572505 69572308 ID=MsG0680033617.01.T01:cds;Parent=MsG0680033617.01.T01
Chr6 CDS 69573020 69573081 69573020 ID=MsG0680033617.01.T01:cds;Parent=MsG0680033617.01.T01
Chr6 CDS 69573179 69573219 69573179 ID=MsG0680033617.01.T01:cds;Parent=MsG0680033617.01.T01
Chr6 CDS 69573609 69573826 69573609 ID=MsG0680033617.01.T01:cds;Parent=MsG0680033617.01.T01
Gene Sequence

>MsG0680033617.01.T01

ATGTCTAGGCCATTGGAACCACTTTCTGTAGGAAGAGTGATAGGAGAAGTTGTTGACATTTTCAATCCAAGTGTAAGAATGAATGTGACATATTCAACCAAGCAAGTTGCAAATGGTCATGAGTTAATGCCTTCTATTGTTATGAACAAGCCAAGGGTTGATATTGGTGGTGATGACATGAGATCTGCTTATACTTTGATCATGACAGACCCAGATGCTCCTAGTCCTAGTGATCCACATTTAAGGGAACATCTCCACTGGATGGTTACAGATATTCCAGGTACCACAGATGTCTCTTTTGGAAATGAGATTGTGGAGTATGAGAATCCAAAGCCAGTGATTGGAATCCATAGATATGTCTTCATCTTGTTCAAGCAGAGAAGGAGACAAACAGTGAGATCCCCATCTTCTAGAGACAATTTCAACACAAGGAGATTCTCACAGGAGAATAACCTTGGTTTACCTGTTGCTGCAGTTTACTTCAATGCTCAAAGAGAAACTGCAGCAAGAAGAAGGTAA

Protein sequence

>MsG0680033617.01.T01

MSRPLEPLSVGRVIGEVVDIFNPSVRMNVTYSTKQVANGHELMPSIVMNKPRVDIGGDDMRSAYTLIMTDPDAPSPSDPHLREHLHWMVTDIPGTTDVSFGNEIVEYENPKPVIGIHRYVFILFKQRRRQTVRSPSSRDNFNTRRFSQENNLGLPVAAVYFNAQRETAARRR*