AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034254.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034254.01.T02 MTR_6g065460 56.897 58 23 2 1 58 147 202 8.04e-17 70.9
MsG0680034254.01.T02 MTR_6g059650 52.174 69 31 2 1 69 145 211 2.53e-15 67.0
MsG0680034254.01.T02 MTR_6g059530 52.778 72 28 4 1 69 135 203 7.29e-14 63.2
MsG0680034254.01.T02 MTR_6g059410 51.389 72 29 4 1 69 134 202 1.45e-13 62.4
MsG0680034254.01.T01 MTR_6g059650 50.909 220 97 8 1 218 1 211 2.34e-51 165
MsG0680034254.01.T01 MTR_6g065460 47.642 212 96 9 1 207 1 202 7.69e-49 159
MsG0680034254.01.T01 MTR_6g059760 43.805 226 106 10 1 218 1 213 2.35e-46 152
MsG0680034254.01.T01 MTR_6g059530 47.706 218 94 9 4 218 3 203 1.89e-45 150
MsG0680034254.01.T01 MTR_6g044780 39.726 219 117 6 1 213 1 210 2.28e-43 145
MsG0680034254.01.T01 MTR_6g045097 39.189 222 119 7 1 215 1 213 5.61e-42 142
MsG0680034254.01.T01 MTR_6g059410 45.413 218 98 11 4 218 3 202 3.63e-40 136
MsG0680034254.01.T01 MTR_6g044810 38.636 220 118 7 1 213 1 210 9.20e-40 136
MsG0680034254.01.T01 MTR_6g045403 40.566 212 111 7 1 207 1 202 1.00e-39 136
MsG0680034254.01.T01 MTR_6g045433 36.681 229 115 8 1 213 1 215 2.15e-38 132
MsG0680034254.01.T01 MTR_6g065460 40.566 212 87 8 1 207 1 178 8.44e-35 122
MsG0680034254.01.T01 MTR_6g059730 40.845 213 109 9 1 207 1 202 3.45e-31 114
MsG0680034254.01.T01 MTR_6g059680 42.857 217 103 9 1 207 1 206 9.14e-31 113
MsG0680034254.01.T01 MTR_6g017225 40.639 219 105 12 1 207 1 206 2.20e-29 112
MsG0680034254.01.T01 MTR_3g463590 37.500 200 112 8 22 218 23 212 4.56e-29 108
MsG0680034254.01.T01 MTR_1g057110 35.484 217 97 9 1 207 1 184 1.79e-28 106
MsG0680034254.01.T01 MTR_6g009650 36.957 230 112 11 1 217 1 210 4.70e-27 103
MsG0680034254.01.T01 MTR_6g066210 37.156 218 110 10 1 207 1 202 5.82e-26 100
MsG0680034254.01.T01 MTR_6g066190 37.004 227 115 12 1 216 1 210 5.60e-24 95.1
MsG0680034254.01.T01 MTR_6g009563 35.909 220 121 10 1 213 1 207 1.23e-22 91.7
MsG0680034254.01.T01 MTR_6g059810 35.185 216 117 9 5 208 6 210 6.86e-22 89.7
MsG0680034254.01.T01 MTR_1g035770 33.495 206 117 9 1 195 1 197 1.02e-19 84.3
MsG0680034254.01.T01 MTR_6g059755 41.379 116 58 7 108 218 20 130 1.23e-15 71.2
MsG0680034254.01.T01 MTR_6g078070 27.451 204 141 5 4 206 3 200 9.38e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034254.01.T01 AT1G17860 29.126 206 128 5 4 206 2 192 1.68e-16 75.1
MsG0680034254.01.T01 AT1G73325 30.097 206 128 7 5 206 7 200 5.13e-12 63.2

Find 50 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAATGTCAAATACTTTATT+TGG 0.154388 6:+84512442 MsG0680034254.01.T01:CDS
TCTACTATGCTAAATTTAAT+TGG 0.190622 6:-84512582 None:intergenic
CCACCAGTTGAAAAGGAATT+TGG 0.236793 6:-84512465 None:intergenic
GCAATGAATACAACATTAAA+TGG 0.249376 6:-84512930 None:intergenic
TTTAAGGATTTAGCACATTC+TGG 0.263488 6:-84512669 None:intergenic
ACTTGTTCTGTAAAGGCTAA+TGG 0.267193 6:-84512390 None:intergenic
CCATTTGACTGACCGCATTA+TGG 0.295536 6:+84512758 MsG0680034254.01.T01:CDS
TACTGGTAAGTGTGTAGATA+TGG 0.306563 6:-84512625 None:intergenic
GGTAAGTGATGTTGGCTTCA+TGG 0.314283 6:-84512328 None:intergenic
ATATCAAACTCAATAAATAC+TGG 0.403900 6:-84512642 None:intergenic
GTCCTTTACTTGTTCTGTAA+AGG 0.421728 6:-84512397 None:intergenic
TGACACAAGGGAGCCGTGGC+AGG 0.463691 6:+84512503 MsG0680034254.01.T01:CDS
AAATCTTGAAGTACAGTAGT+TGG 0.464871 6:-84512537 None:intergenic
CAGTCCACCACCAGTTGAAA+AGG 0.465235 6:-84512472 None:intergenic
ATTGCTGATAACTCTGGATC+TGG 0.469718 6:+84512948 MsG0680034254.01.T01:CDS
GTATTCATTGCTGATAACTC+TGG 0.472443 6:+84512942 MsG0680034254.01.T01:CDS
GAACAAGTAAAGGACACAAA+TGG 0.481025 6:+84512405 MsG0680034254.01.T01:CDS
CCATAATGCGGTCAGTCAAA+TGG 0.486325 6:-84512758 None:intergenic
AATGTGCTAAATCCTTAAAG+TGG 0.492977 6:+84512673 MsG0680034254.01.T01:CDS
CCGATAGCCACCCATGGTAG+AGG 0.513253 6:-84512720 None:intergenic
CCTCTACCATGGGTGGCTAT+CGG 0.514699 6:+84512720 MsG0680034254.01.T01:CDS
AGGCAAAGAGGAGCAAAGAA+AGG 0.526841 6:-84512353 None:intergenic
ACTGGTAAGTGTGTAGATAT+GGG 0.531982 6:-84512624 None:intergenic
GGAAGACATCGTGATGAGAA+AGG 0.532879 6:+84512876 MsG0680034254.01.T01:CDS
AGCCTTTACAGAACAAGTAA+AGG 0.533453 6:+84512395 MsG0680034254.01.T01:CDS
TTTCAACTGGTGGTGGACTG+AGG 0.533947 6:+84512475 MsG0680034254.01.T01:CDS
GGTGGCTATCGGTGATTTCG+CGG 0.534492 6:+84512731 MsG0680034254.01.T01:CDS
GAAAGGGTGGTAAGTGATGT+TGG 0.534750 6:-84512336 None:intergenic
CCGACATCAAAACAAGTATC+CGG 0.539284 6:-84512855 None:intergenic
TAAATTTAATTGGCAAGCCA+TGG 0.548633 6:-84512572 None:intergenic
AACATTCAGAAACATGGTAC+AGG 0.549070 6:+84512792 MsG0680034254.01.T01:CDS
TAATGGAAAGTTTGTGGTGA+AGG 0.552923 6:-84512373 None:intergenic
GGACATTCGTCGGCCTGCCA+CGG 0.554761 6:-84512516 None:intergenic
AAAGGCTAATGGAAAGTTTG+TGG 0.562122 6:-84512379 None:intergenic
TTCTTTAACATTCAGAAACA+TGG 0.562425 6:+84512786 MsG0680034254.01.T01:CDS
GCTATCGGTGATTTCGCGGA+TGG 0.562456 6:+84512735 MsG0680034254.01.T01:CDS
GGCAAAGAGGAGCAAAGAAA+GGG 0.563130 6:-84512352 None:intergenic
TTGACTGACCGCATTATGGA+AGG 0.565070 6:+84512762 MsG0680034254.01.T01:CDS
ACTGGTGGTGGACTGAGGAT+TGG 0.581829 6:+84512480 MsG0680034254.01.T01:CDS
CGATAGCCACCCATGGTAGA+GGG 0.583970 6:-84512719 None:intergenic
GACTGAGGATTGGTGACACA+AGG 0.587342 6:+84512490 MsG0680034254.01.T01:CDS
TACAGTAGTTGGACATTCGT+CGG 0.602808 6:-84512526 None:intergenic
ATGTGCTAAATCCTTAAAGT+GGG 0.605173 6:+84512674 MsG0680034254.01.T01:CDS
TTGGTGACACAAGGGAGCCG+TGG 0.623671 6:+84512499 MsG0680034254.01.T01:CDS
AAAGAGGAGCAAAGAAAGGG+TGG 0.645158 6:-84512349 None:intergenic
TAAAGAAACCTTCCATAATG+CGG 0.661954 6:-84512770 None:intergenic
AGTTTGTGGTGAAGGCAAAG+AGG 0.668922 6:-84512365 None:intergenic
ACATCAAAACAAGTATCCGG+TGG 0.675645 6:-84512852 None:intergenic
AAATCACCGATAGCCACCCA+TGG 0.684221 6:-84512726 None:intergenic
ACTGAGGATTGGTGACACAA+GGG 0.706089 6:+84512491 MsG0680034254.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCAAACTCAATAAATAC+TGG - Chr6:84512645-84512664 None:intergenic 20.0%
!! ATTTGACATTAGCAAAAATA+GGG - Chr6:84512434-84512453 None:intergenic 20.0%
!! CTAATGTCAAATACTTTATT+TGG + Chr6:84512442-84512461 MsG0680034254.01.T01:CDS 20.0%
!! TATTTGACATTAGCAAAAAT+AGG - Chr6:84512435-84512454 None:intergenic 20.0%
!! TCTACTATGCTAAATTTAAT+TGG - Chr6:84512585-84512604 None:intergenic 20.0%
! GCAATGAATACAACATTAAA+TGG - Chr6:84512933-84512952 None:intergenic 25.0%
! TTCTTTAACATTCAGAAACA+TGG + Chr6:84512786-84512805 MsG0680034254.01.T01:CDS 25.0%
! TTGAAAAAATTCCCACTTTA+AGG - Chr6:84512688-84512707 None:intergenic 25.0%
AAATCTTGAAGTACAGTAGT+TGG - Chr6:84512540-84512559 None:intergenic 30.0%
AATGTGCTAAATCCTTAAAG+TGG + Chr6:84512673-84512692 MsG0680034254.01.T01:CDS 30.0%
ATGTGCTAAATCCTTAAAGT+GGG + Chr6:84512674-84512693 MsG0680034254.01.T01:CDS 30.0%
TAAAGAAACCTTCCATAATG+CGG - Chr6:84512773-84512792 None:intergenic 30.0%
TAAATTTAATTGGCAAGCCA+TGG - Chr6:84512575-84512594 None:intergenic 30.0%
TTTAAGGATTTAGCACATTC+TGG - Chr6:84512672-84512691 None:intergenic 30.0%
! GATTTTTCTCAAAATGACCA+TGG + Chr6:84512555-84512574 MsG0680034254.01.T01:CDS 30.0%
AAAGGCTAATGGAAAGTTTG+TGG - Chr6:84512382-84512401 None:intergenic 35.0%
AACATTCAGAAACATGGTAC+AGG + Chr6:84512792-84512811 MsG0680034254.01.T01:CDS 35.0%
ACTGGTAAGTGTGTAGATAT+GGG - Chr6:84512627-84512646 None:intergenic 35.0%
ACTTGTTCTGTAAAGGCTAA+TGG - Chr6:84512393-84512412 None:intergenic 35.0%
AGCCTTTACAGAACAAGTAA+AGG + Chr6:84512395-84512414 MsG0680034254.01.T01:CDS 35.0%
GAACAAGTAAAGGACACAAA+TGG + Chr6:84512405-84512424 MsG0680034254.01.T01:CDS 35.0%
GTATTCATTGCTGATAACTC+TGG + Chr6:84512942-84512961 MsG0680034254.01.T01:CDS 35.0%
GTCCTTTACTTGTTCTGTAA+AGG - Chr6:84512400-84512419 None:intergenic 35.0%
TAATGGAAAGTTTGTGGTGA+AGG - Chr6:84512376-84512395 None:intergenic 35.0%
TACTGGTAAGTGTGTAGATA+TGG - Chr6:84512628-84512647 None:intergenic 35.0%
!!! AAGATTTTTTCCCTCTACCA+TGG + Chr6:84512709-84512728 MsG0680034254.01.T01:CDS 35.0%
!!! AGATTTTTTCCCTCTACCAT+GGG + Chr6:84512710-84512729 MsG0680034254.01.T01:CDS 35.0%
ACATCAAAACAAGTATCCGG+TGG - Chr6:84512855-84512874 None:intergenic 40.0%
ATTGCTGATAACTCTGGATC+TGG + Chr6:84512948-84512967 MsG0680034254.01.T01:CDS 40.0%
CCACCAGTTGAAAAGGAATT+TGG - Chr6:84512468-84512487 None:intergenic 40.0%
CCGACATCAAAACAAGTATC+CGG - Chr6:84512858-84512877 None:intergenic 40.0%
TACAGTAGTTGGACATTCGT+CGG - Chr6:84512529-84512548 None:intergenic 40.0%
! AATTCCTTTTCAACTGGTGG+TGG + Chr6:84512468-84512487 MsG0680034254.01.T01:CDS 40.0%
! CCAAATTCCTTTTCAACTGG+TGG + Chr6:84512465-84512484 MsG0680034254.01.T01:CDS 40.0%
! TGGCCAAATTCCTTTTCAAC+TGG + Chr6:84512462-84512481 MsG0680034254.01.T01:CDS 40.0%
!!! CCGGATACTTGTTTTGATGT+CGG + Chr6:84512855-84512874 MsG0680034254.01.T01:CDS 40.0%
AAAGAGGAGCAAAGAAAGGG+TGG - Chr6:84512352-84512371 None:intergenic 45.0%
AGGCAAAGAGGAGCAAAGAA+AGG - Chr6:84512356-84512375 None:intergenic 45.0%
AGTTTGTGGTGAAGGCAAAG+AGG - Chr6:84512368-84512387 None:intergenic 45.0%
CCATAATGCGGTCAGTCAAA+TGG - Chr6:84512761-84512780 None:intergenic 45.0%
CCATTTGACTGACCGCATTA+TGG + Chr6:84512758-84512777 MsG0680034254.01.T01:CDS 45.0%
GAAAGGGTGGTAAGTGATGT+TGG - Chr6:84512339-84512358 None:intergenic 45.0%
GGAAGACATCGTGATGAGAA+AGG + Chr6:84512876-84512895 MsG0680034254.01.T01:CDS 45.0%
GGCAAAGAGGAGCAAAGAAA+GGG - Chr6:84512355-84512374 None:intergenic 45.0%
TTGACTGACCGCATTATGGA+AGG + Chr6:84512762-84512781 MsG0680034254.01.T01:CDS 45.0%
! ACTGAGGATTGGTGACACAA+GGG + Chr6:84512491-84512510 MsG0680034254.01.T01:CDS 45.0%
!!! AACAGACGCTTTTTCTCCAC+CGG + Chr6:84512836-84512855 MsG0680034254.01.T01:CDS 45.0%
!!! TTTTTTCCCTCTACCATGGG+TGG + Chr6:84512713-84512732 MsG0680034254.01.T01:CDS 45.0%
AAATCACCGATAGCCACCCA+TGG - Chr6:84512729-84512748 None:intergenic 50.0%
CAGTCCACCACCAGTTGAAA+AGG - Chr6:84512475-84512494 None:intergenic 50.0%
! GACTGAGGATTGGTGACACA+AGG + Chr6:84512490-84512509 MsG0680034254.01.T01:CDS 50.0%
!! TTTCAACTGGTGGTGGACTG+AGG + Chr6:84512475-84512494 MsG0680034254.01.T01:CDS 50.0%
CCTCTACCATGGGTGGCTAT+CGG + Chr6:84512720-84512739 MsG0680034254.01.T01:CDS 55.0%
CGATAGCCACCCATGGTAGA+GGG - Chr6:84512722-84512741 None:intergenic 55.0%
GCTATCGGTGATTTCGCGGA+TGG + Chr6:84512735-84512754 MsG0680034254.01.T01:CDS 55.0%
GGTGGCTATCGGTGATTTCG+CGG + Chr6:84512731-84512750 MsG0680034254.01.T01:CDS 55.0%
!! ACTGGTGGTGGACTGAGGAT+TGG + Chr6:84512480-84512499 MsG0680034254.01.T01:CDS 55.0%
CCGATAGCCACCCATGGTAG+AGG - Chr6:84512723-84512742 None:intergenic 60.0%
! TTGGTGACACAAGGGAGCCG+TGG + Chr6:84512499-84512518 MsG0680034254.01.T01:CDS 60.0%
GGACATTCGTCGGCCTGCCA+CGG - Chr6:84512519-84512538 None:intergenic 65.0%
TGACACAAGGGAGCCGTGGC+AGG + Chr6:84512503-84512522 MsG0680034254.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 84512330 84512986 84512330 ID=MsG0680034254.01;Name=MsG0680034254.01
Chr6 mRNA 84512330 84512986 84512330 ID=MsG0680034254.01.T01;Parent=MsG0680034254.01;Name=MsG0680034254.01.T01;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|218
Chr6 exon 84512330 84512986 84512330 ID=MsG0680034254.01.T01:exon:19275;Parent=MsG0680034254.01.T01
Chr6 CDS 84512330 84512986 84512330 ID=MsG0680034254.01.T01:cds;Parent=MsG0680034254.01.T01
Chr6 mRNA 84512777 84512986 84512777 ID=MsG0680034254.01.T02;Parent=MsG0680034254.01;Name=MsG0680034254.01.T02;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|0|1|0|69
Chr6 exon 84512777 84512986 84512777 ID=MsG0680034254.01.T02:exon:19276;Parent=MsG0680034254.01.T02
Chr6 CDS 84512777 84512986 84512777 ID=MsG0680034254.01.T02:cds;Parent=MsG0680034254.01.T02
Gene Sequence

>MsG0680034254.01.T02

ATGGAAGGTTTCTTTAACATTCAGAAACATGGTACAGGATACAAGTTAGTGTACTGTTTAACAGACGCTTTTTCTCCACCGGATACTTGTTTTGATGTCGGAAGACATCGTGATGAGAAAGGAAATCGTCTCGTTCTAACTGAAAATAATGATCCATTTAATGTTGTATTCATTGCTGATAACTCTGGATCTGGAAGATCTGTTGTTTGA

>MsG0680034254.01.T01

ATGAAGCCAACATCACTTACCACCCTTTCTTTGCTCCTCTTTGCCTTCACCACAAACTTTCCATTAGCCTTTACAGAACAAGTAAAGGACACAAATGGAAACCCTATTTTTGCTAATGTCAAATACTTTATTTGGCCAAATTCCTTTTCAACTGGTGGTGGACTGAGGATTGGTGACACAAGGGAGCCGTGGCAGGCCGACGAATGTCCAACTACTGTACTTCAAGATTTTTCTCAAAATGACCATGGCTTGCCAATTAAATTTAGCATAGTAGAAACAAGCAACAGTGCAAGTCCCATATCTACACACTTACCAGTATTTATTGAGTTTGATATAAAGCCAGAATGTGCTAAATCCTTAAAGTGGGAATTTTTTCAACAAGATTTTTTCCCTCTACCATGGGTGGCTATCGGTGATTTCGCGGATGGCCATTTGACTGACCGCATTATGGAAGGTTTCTTTAACATTCAGAAACATGGTACAGGATACAAGTTAGTGTACTGTTTAACAGACGCTTTTTCTCCACCGGATACTTGTTTTGATGTCGGAAGACATCGTGATGAGAAAGGAAATCGTCTCGTTCTAACTGAAAATAATGATCCATTTAATGTTGTATTCATTGCTGATAACTCTGGATCTGGAAGATCTGTTGTTTGA

Protein sequence

>MsG0680034254.01.T02

MEGFFNIQKHGTGYKLVYCLTDAFSPPDTCFDVGRHRDEKGNRLVLTENNDPFNVVFIADNSGSGRSVV*

>MsG0680034254.01.T01

MKPTSLTTLSLLLFAFTTNFPLAFTEQVKDTNGNPIFANVKYFIWPNSFSTGGGLRIGDTREPWQADECPTTVLQDFSQNDHGLPIKFSIVETSNSASPISTHLPVFIEFDIKPECAKSLKWEFFQQDFFPLPWVAIGDFADGHLTDRIMEGFFNIQKHGTGYKLVYCLTDAFSPPDTCFDVGRHRDEKGNRLVLTENNDPFNVVFIADNSGSGRSVV*