AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037153.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037153.01.T01 MTR_1g050478 86.154 130 15 1 6 135 378 504 3.29e-72 225
MsG0780037153.01.T01 MTR_3g110740 74.803 127 32 0 8 134 396 522 6.67e-62 199
MsG0780037153.01.T01 MTR_6g016705 66.667 138 34 3 6 134 217 351 1.25e-51 169
MsG0780037153.01.T01 MTR_6g025290 67.939 131 31 3 6 134 245 366 1.86e-50 166
MsG0780037153.01.T01 MTR_7g029535 67.969 128 40 1 8 134 429 556 3.01e-50 169
MsG0780037153.01.T01 MTR_5g046730 64.885 131 41 2 6 134 252 379 5.13e-50 165
MsG0780037153.01.T01 MTR_4g059340 68.033 122 36 1 6 127 379 497 1.11e-49 166
MsG0780037153.01.T01 MTR_6g016725 66.667 129 42 1 7 134 430 558 5.04e-49 165
MsG0780037153.01.T01 MTR_6g016685 64.885 131 35 3 6 134 168 289 2.71e-48 158
MsG0780037153.01.T01 MTR_6g016905 63.281 128 46 1 8 135 369 495 8.49e-48 161
MsG0780037153.01.T01 MTR_6g042905 65.649 131 39 3 6 134 235 361 1.18e-47 159
MsG0780037153.01.T01 MTR_6g016750 63.846 130 40 3 6 135 244 366 5.86e-47 157
MsG0780037153.01.T01 MTR_6g016720 64.167 120 38 2 7 124 101 217 1.51e-46 150
MsG0780037153.01.T01 MTR_6g022820 63.359 131 43 2 6 134 286 413 2.41e-45 154
MsG0780037153.01.T01 MTR_3g036270 67.969 128 40 1 8 134 431 558 9.97e-45 154
MsG0780037153.01.T01 MTR_7g044870 62.903 124 44 1 14 135 216 339 3.32e-44 149
MsG0780037153.01.T01 MTR_6g016760 63.158 114 41 1 22 135 1 113 7.82e-44 144
MsG0780037153.01.T01 MTR_6g016915 59.848 132 47 3 6 135 202 329 1.08e-43 147
MsG0780037153.01.T01 MTR_3g006050 63.359 131 45 2 7 134 379 509 5.70e-43 149
MsG0780037153.01.T01 MTR_4g133640 60.870 138 47 4 1 134 372 506 6.82e-42 146
MsG0780037153.01.T01 MTR_6g016740 58.333 132 33 3 6 135 209 320 6.87e-42 142
MsG0780037153.01.T01 MTR_5g095830 63.710 124 41 3 14 134 394 516 3.73e-41 144
MsG0780037153.01.T01 MTR_4g006240 60.902 133 46 3 6 135 357 486 8.88e-41 143
MsG0780037153.01.T01 MTR_2g007590 60.145 138 48 4 1 134 372 506 9.76e-41 143
MsG0780037153.01.T01 MTR_6g016910 61.654 133 45 3 6 135 232 361 1.56e-40 140
MsG0780037153.01.T01 MTR_4g024200 61.290 124 45 2 14 134 261 384 2.55e-40 140
MsG0780037153.01.T01 MTR_5g095810 67.925 106 33 1 31 135 113 218 3.22e-40 136
MsG0780037153.01.T01 MTR_3g110148 67.308 104 34 0 32 135 189 292 4.47e-40 138
MsG0780037153.01.T01 MTR_2g078550 66.038 106 34 2 30 134 3 107 4.68e-39 129
MsG0780037153.01.T01 MTR_5g075860 63.710 124 41 3 14 134 353 475 5.03e-39 138
MsG0780037153.01.T01 MTR_4g006290 64.762 105 36 1 30 134 259 362 1.40e-37 132
MsG0780037153.01.T01 MTR_4g006210 59.231 130 42 4 14 134 195 322 3.57e-37 132
MsG0780037153.01.T01 MTR_6g027420 63.415 123 40 4 15 134 270 390 1.98e-36 130
MsG0780037153.01.T01 MTR_4g006450 57.364 129 38 2 8 134 357 470 2.38e-36 130
MsG0780037153.01.T01 MTR_8g446780 55.738 122 49 2 14 134 65 182 1.09e-35 123
MsG0780037153.01.T01 MTR_4g021765 53.659 123 55 2 14 134 124 246 1.41e-35 125
MsG0780037153.01.T01 MTR_8g045150 57.252 131 37 3 6 134 292 405 1.25e-34 125
MsG0780037153.01.T01 MTR_1g115380 54.887 133 55 3 7 135 346 477 6.36e-34 124
MsG0780037153.01.T01 MTR_5g067480 50.000 120 57 1 2 118 52 171 3.06e-32 114
MsG0780037153.01.T01 MTR_3g083280 50.000 130 59 3 7 134 297 422 4.73e-32 118
MsG0780037153.01.T01 MTR_0295s0010 66.304 92 29 2 46 135 8 99 1.37e-31 113
MsG0780037153.01.T01 MTR_4g005810 62.000 100 37 1 36 134 11 110 2.37e-31 113
MsG0780037153.01.T01 MTR_5g088250 45.113 133 67 3 6 135 328 457 3.81e-31 116
MsG0780037153.01.T01 MTR_3g465770 54.032 124 49 4 14 133 372 491 4.94e-31 116
MsG0780037153.01.T01 MTR_5g430490 49.618 131 61 2 6 134 368 495 8.31e-31 115
MsG0780037153.01.T01 MTR_1g115390 52.632 133 57 3 7 135 185 315 1.53e-30 112
MsG0780037153.01.T01 MTR_7g029070 82.759 58 10 0 10 67 1 58 1.94e-30 104
MsG0780037153.01.T01 MTR_4g435338 53.913 115 50 2 22 135 121 233 5.97e-30 110
MsG0780037153.01.T01 MTR_8g017160 51.240 121 57 2 15 134 387 506 2.71e-28 108
MsG0780037153.01.T01 MTR_3g074570 47.967 123 57 3 14 133 251 369 1.38e-27 105
MsG0780037153.01.T01 MTR_8g037780 50.000 130 59 4 6 134 376 500 2.30e-27 105
MsG0780037153.01.T01 MTR_4g008450 52.137 117 51 3 20 135 100 212 4.24e-27 102
MsG0780037153.01.T01 MTR_4g008430 58.163 98 38 2 8 102 206 303 8.12e-27 101
MsG0780037153.01.T01 MTR_8g076010 59.794 97 37 2 40 135 258 353 1.06e-26 103
MsG0780037153.01.T01 MTR_4g009030 44.800 125 65 3 14 135 358 481 6.37e-26 102
MsG0780037153.01.T01 MTR_4g021835 49.580 119 50 2 26 134 255 373 1.14e-25 100
MsG0780037153.01.T01 MTR_7g029070 81.633 49 9 0 10 58 1 49 4.07e-24 88.6
MsG0780037153.01.T01 MTR_2g104970 56.250 80 34 1 57 135 3 82 7.62e-24 90.9
MsG0780037153.01.T01 MTR_4g024220 54.651 86 38 1 49 133 28 113 8.49e-24 91.7
MsG0780037153.01.T01 MTR_1g023530 54.286 105 42 3 6 107 260 361 4.23e-23 93.6
MsG0780037153.01.T01 MTR_4g008420 61.111 72 27 1 65 135 146 217 9.77e-22 88.6
MsG0780037153.01.T01 MTR_6g054950 45.385 130 19 3 6 135 234 311 2.19e-21 88.2
MsG0780037153.01.T01 MTR_4g019320 43.939 132 52 3 7 135 358 470 2.58e-21 89.0
MsG0780037153.01.T01 MTR_1g075040 52.475 101 42 3 6 103 266 363 4.29e-21 87.4
MsG0780037153.01.T01 MTR_6g016900 75.472 53 13 0 83 135 24 76 1.15e-20 84.7
MsG0780037153.01.T01 MTR_3g073390 46.087 115 58 2 8 122 332 442 1.94e-19 83.6
MsG0780037153.01.T01 MTR_4g008480 48.077 104 48 2 6 106 140 240 2.88e-19 81.6
MsG0780037153.01.T01 MTR_1g069145 49.057 106 45 4 6 105 323 425 4.21e-19 82.4
MsG0780037153.01.T01 MTR_1g069090 44.444 135 43 5 6 134 93 201 4.67e-19 80.9
MsG0780037153.01.T01 MTR_3g081430 55.056 89 36 3 14 102 190 274 8.95e-19 80.5
MsG0780037153.01.T01 MTR_4g008330 56.944 72 30 1 65 135 1 72 9.50e-19 77.8
MsG0780037153.01.T01 MTR_4g012820 62.500 64 23 1 75 137 125 188 3.01e-17 74.3
MsG0780037153.01.T01 MTR_3g110150 48.315 89 45 1 47 134 114 202 3.91e-17 75.9
MsG0780037153.01.T01 MTR_5g087540 37.302 126 48 2 14 134 55 154 7.40e-17 73.9
MsG0780037153.01.T01 MTR_3g074000 50.649 77 37 1 8 84 361 436 7.13e-16 73.2
MsG0780037153.01.T01 MTR_4g006250 59.016 61 24 1 75 134 1 61 2.85e-15 68.6
MsG0780037153.01.T01 MTR_8g045170 45.455 88 41 2 5 88 237 321 2.87e-15 71.2
MsG0780037153.01.T01 MTR_1g028400 53.333 75 33 1 14 86 315 389 7.73e-15 70.5
MsG0780037153.01.T01 MTR_6g080560 51.351 74 32 2 65 134 144 217 1.76e-14 68.6
MsG0780037153.01.T01 MTR_4g129140 35.938 128 31 2 7 134 307 383 2.52e-14 68.9
MsG0780037153.01.T01 MTR_4g006280 49.333 75 37 1 62 135 181 255 3.77e-14 67.8
MsG0780037153.01.T01 MTR_4g008440 50.685 73 34 2 63 134 139 210 4.32e-14 67.0
MsG0780037153.01.T01 MTR_1g069120 46.316 95 43 3 6 95 323 414 7.09e-14 67.4
MsG0780037153.01.T01 MTR_8g060730 36.220 127 41 4 45 134 157 280 7.31e-13 64.3
MsG0780037153.01.T01 MTR_4g008490 48.780 82 32 4 63 135 1 81 2.23e-12 61.2
MsG0780037153.01.T01 MTR_4g009055 41.667 96 50 3 16 105 258 353 3.17e-12 62.8
MsG0780037153.01.T01 MTR_8g067230 70.833 48 13 1 87 134 180 226 3.38e-12 62.4
MsG0780037153.01.T01 MTR_4g008600 51.316 76 29 2 15 88 154 223 4.50e-12 61.6
MsG0780037153.01.T01 MTR_4g008340 46.575 73 37 2 63 134 166 237 9.91e-12 61.2
MsG0780037153.01.T01 MTR_4g008400 47.945 73 36 2 63 134 138 209 1.41e-11 60.5
MsG0780037153.01.T01 MTR_7g100890 37.143 140 73 7 6 137 222 354 3.14e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGATTCTTTGCCGGATTT+TGG 0.189785 7:+20759013 MsG0780037153.01.T01:CDS
TCAATTAAAGTCTCTCTATT+TGG 0.216940 7:+20759045 MsG0780037153.01.T01:CDS
AAATCAAGCAGCTTCAAATT+AGG 0.300474 7:-20759121 None:intergenic
GAGATCAAAATGGAAAATAT+AGG 0.343659 7:+20758971 MsG0780037153.01.T01:CDS
TCAGCCTTGTTTGCACTTGT+AGG 0.345197 7:+20758930 MsG0780037153.01.T01:CDS
TAAGGAGGTGTTCTAATATC+AGG 0.348599 7:+20759185 MsG0780037153.01.T01:CDS
AGGGAATTCTGATTCTTTGC+CGG 0.357335 7:+20759006 MsG0780037153.01.T01:CDS
TTCACTTAGTGAGATCAAAA+TGG 0.401122 7:+20758961 MsG0780037153.01.T01:CDS
AGATCAAAATGGAAAATATA+GGG 0.431656 7:+20758972 MsG0780037153.01.T01:CDS
GAACTCTTGCCATCACATAT+CGG 0.435228 7:+20759144 MsG0780037153.01.T01:CDS
TATAAACCTTAGACAATGTT+GGG 0.447233 7:+20758896 None:intergenic
TCTATTTGGCTGAGAATACA+TGG 0.472085 7:+20759059 MsG0780037153.01.T01:CDS
AAGTTCAACACCTCCAGCTT+GGG 0.475609 7:-20759265 None:intergenic
AATATAGGGAGCAAGGGTGT+AGG 0.497229 7:+20758986 MsG0780037153.01.T01:CDS
AAATAGAGAGACTTTAATTG+AGG 0.504107 7:-20759043 None:intergenic
TGAGGGTATACACCAAAATC+CGG 0.505757 7:-20759025 None:intergenic
TGTTCAAGAGTGAATCTACT+TGG 0.514036 7:+20759226 MsG0780037153.01.T01:CDS
CAAGTTCAACACCTCCAGCT+TGG 0.519812 7:-20759266 None:intergenic
AATAGAGAGACTTTAATTGA+GGG 0.527352 7:-20759042 None:intergenic
GAACTTCGTACTTCCCAAGC+TGG 0.540661 7:+20759252 MsG0780037153.01.T01:CDS
AATGGAAAATATAGGGAGCA+AGG 0.543928 7:+20758979 MsG0780037153.01.T01:CDS
ATATAGGGAGCAAGGGTGTA+GGG 0.547334 7:+20758987 MsG0780037153.01.T01:CDS
TAGATTCACTCTTGAACAGT+GGG 0.559421 7:-20759221 None:intergenic
AATTCCTACAAGTGCAAACA+AGG 0.568318 7:-20758934 None:intergenic
ATGGAAAATATAGGGAGCAA+GGG 0.581512 7:+20758980 MsG0780037153.01.T01:CDS
GTTGAACTTGTCATATACAA+AGG 0.585440 7:+20759279 MsG0780037153.01.T01:CDS
GTAGATTCACTCTTGAACAG+TGG 0.614201 7:-20759222 None:intergenic
TCTTGCCATCACATATCGGA+AGG 0.639103 7:+20759148 MsG0780037153.01.T01:CDS
AAATGCCTTCCGATATGTGA+TGG 0.665635 7:-20759153 None:intergenic
CTTCGTACTTCCCAAGCTGG+AGG 0.684702 7:+20759255 MsG0780037153.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATCAAAATGGAAAATATA+GGG + Chr7:20758972-20758991 MsG0780037153.01.T01:CDS 20.0%
! AAATAGAGAGACTTTAATTG+AGG - Chr7:20759046-20759065 None:intergenic 25.0%
! AATAGAGAGACTTTAATTGA+GGG - Chr7:20759045-20759064 None:intergenic 25.0%
! GAGATCAAAATGGAAAATAT+AGG + Chr7:20758971-20758990 MsG0780037153.01.T01:CDS 25.0%
! TCAATTAAAGTCTCTCTATT+TGG + Chr7:20759045-20759064 MsG0780037153.01.T01:CDS 25.0%
AAATCAAGCAGCTTCAAATT+AGG - Chr7:20759124-20759143 None:intergenic 30.0%
CAGCTTCAAATTAGGAAAAA+TGG - Chr7:20759116-20759135 None:intergenic 30.0%
GTTGAACTTGTCATATACAA+AGG + Chr7:20759279-20759298 MsG0780037153.01.T01:CDS 30.0%
TTCACTTAGTGAGATCAAAA+TGG + Chr7:20758961-20758980 MsG0780037153.01.T01:CDS 30.0%
!! AAGGCATTTGTCAAGTTTTA+AGG + Chr7:20759167-20759186 MsG0780037153.01.T01:CDS 30.0%
!! ATTTTGATCTCACTAAGTGA+AGG - Chr7:20758962-20758981 None:intergenic 30.0%
AATGGAAAATATAGGGAGCA+AGG + Chr7:20758979-20758998 MsG0780037153.01.T01:CDS 35.0%
AATTCCTACAAGTGCAAACA+AGG - Chr7:20758937-20758956 None:intergenic 35.0%
ATGGAAAATATAGGGAGCAA+GGG + Chr7:20758980-20758999 MsG0780037153.01.T01:CDS 35.0%
TAGATTCACTCTTGAACAGT+GGG - Chr7:20759224-20759243 None:intergenic 35.0%
TCTATTTGGCTGAGAATACA+TGG + Chr7:20759059-20759078 MsG0780037153.01.T01:CDS 35.0%
TGTTCAAGAGTGAATCTACT+TGG + Chr7:20759226-20759245 MsG0780037153.01.T01:CDS 35.0%
!! TAAGGAGGTGTTCTAATATC+AGG + Chr7:20759185-20759204 MsG0780037153.01.T01:CDS 35.0%
!!! GCATTTGTCAAGTTTTAAGG+AGG + Chr7:20759170-20759189 MsG0780037153.01.T01:CDS 35.0%
AAATGCCTTCCGATATGTGA+TGG - Chr7:20759156-20759175 None:intergenic 40.0%
AGGGAATTCTGATTCTTTGC+CGG + Chr7:20759006-20759025 MsG0780037153.01.T01:CDS 40.0%
GAACTCTTGCCATCACATAT+CGG + Chr7:20759144-20759163 MsG0780037153.01.T01:CDS 40.0%
GTAGATTCACTCTTGAACAG+TGG - Chr7:20759225-20759244 None:intergenic 40.0%
! TCTGATTCTTTGCCGGATTT+TGG + Chr7:20759013-20759032 MsG0780037153.01.T01:CDS 40.0%
! TGAGGGTATACACCAAAATC+CGG - Chr7:20759028-20759047 None:intergenic 40.0%
AAGTTCAACACCTCCAGCTT+GGG - Chr7:20759268-20759287 None:intergenic 45.0%
TCTTGCCATCACATATCGGA+AGG + Chr7:20759148-20759167 MsG0780037153.01.T01:CDS 45.0%
! AATATAGGGAGCAAGGGTGT+AGG + Chr7:20758986-20759005 MsG0780037153.01.T01:CDS 45.0%
! ATATAGGGAGCAAGGGTGTA+GGG + Chr7:20758987-20759006 MsG0780037153.01.T01:CDS 45.0%
!! TCAGCCTTGTTTGCACTTGT+AGG + Chr7:20758930-20758949 MsG0780037153.01.T01:CDS 45.0%
CAAGTTCAACACCTCCAGCT+TGG - Chr7:20759269-20759288 None:intergenic 50.0%
GAACTTCGTACTTCCCAAGC+TGG + Chr7:20759252-20759271 MsG0780037153.01.T01:CDS 50.0%
CTTCGTACTTCCCAAGCTGG+AGG + Chr7:20759255-20759274 MsG0780037153.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 20758911 20759324 20758911 ID=MsG0780037153.01;Name=MsG0780037153.01
Chr7 mRNA 20758911 20759324 20758911 ID=MsG0780037153.01.T01;Parent=MsG0780037153.01;Name=MsG0780037153.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|137
Chr7 exon 20758911 20759324 20758911 ID=MsG0780037153.01.T01:exon:5092;Parent=MsG0780037153.01.T01
Chr7 CDS 20758911 20759324 20758911 ID=MsG0780037153.01.T01:cds;Parent=MsG0780037153.01.T01
Gene Sequence

>MsG0780037153.01.T01

ATGTTGGGAGATCACATATTCAGCCTTGTTTGCACTTGTAGGAATTGTCCTTCACTTAGTGAGATCAAAATGGAAAATATAGGGAGCAAGGGTGTAGGGAATTCTGATTCTTTGCCGGATTTTGGTGTATACCCTCAATTAAAGTCTCTCTATTTGGCTGAGAATACATGGTTAAGTGATGAACACATCATAATGTTCTCTTCCATTTTTCCTAATTTGAAGCTGCTTGATTTGAACTCTTGCCATCACATATCGGAAGGCATTTGTCAAGTTTTAAGGAGGTGTTCTAATATCAGGCATTTGAACTTAGCCCACTGTTCAAGAGTGAATCTACTTGGAGTGAACTTCGTACTTCCCAAGCTGGAGGTGTTGAACTTGTCATATACAAAGGTTGATGATGAAAACTCTATGTGA

Protein sequence

>MsG0780037153.01.T01

MLGDHIFSLVCTCRNCPSLSEIKMENIGSKGVGNSDSLPDFGVYPQLKSLYLAENTWLSDEHIIMFSSIFPNLKLLDLNSCHHISEGICQVLRRCSNIRHLNLAHCSRVNLLGVNFVLPKLEVLNLSYTKVDDENSM*