Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039026.01.T01 | XP_013449064.1 | 85.961 | 463 | 64 | 1 | 1 | 462 | 1 | 463 | 0 | 803 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039026.01.T01 | A0A072U0X9 | 85.961 | 463 | 64 | 1 | 1 | 462 | 1 | 463 | 0.0 | 803 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580027745.01 | MsG0780039026.01 | 0.812276 | 4.599326e-51 | 2.911748e-48 |
| MsG0780038997.01 | MsG0780039026.01 | 0.824626 | 7.289569e-54 | 6.492877e-51 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039026.01.T01 | MTR_7g066340 | 85.961 | 463 | 64 | 1 | 1 | 462 | 1 | 463 | 0.0 | 803 |
| MsG0780039026.01.T01 | MTR_7g066310 | 68.722 | 454 | 78 | 3 | 9 | 462 | 4 | 393 | 0.0 | 594 |
| MsG0780039026.01.T01 | MTR_7g066350 | 65.714 | 455 | 141 | 7 | 1 | 449 | 1 | 446 | 0.0 | 563 |
| MsG0780039026.01.T01 | MTR_7g066280 | 66.508 | 421 | 69 | 2 | 91 | 461 | 1 | 399 | 0.0 | 538 |
| MsG0780039026.01.T01 | MTR_1g041315 | 62.192 | 447 | 148 | 6 | 7 | 436 | 9 | 451 | 0.0 | 526 |
| MsG0780039026.01.T01 | MTR_7g066260 | 57.906 | 468 | 182 | 7 | 6 | 462 | 9 | 472 | 2.04e-180 | 515 |
| MsG0780039026.01.T01 | MTR_7g066570 | 57.613 | 486 | 160 | 9 | 3 | 452 | 5 | 480 | 2.40e-170 | 492 |
| MsG0780039026.01.T01 | MTR_7g066400 | 56.438 | 466 | 154 | 7 | 1 | 449 | 1 | 434 | 3.21e-166 | 477 |
| MsG0780039026.01.T01 | MTR_7g066290 | 56.550 | 458 | 157 | 10 | 6 | 453 | 9 | 434 | 3.65e-154 | 446 |
| MsG0780039026.01.T01 | MTR_7g012680 | 56.223 | 466 | 163 | 9 | 3 | 452 | 5 | 445 | 1.89e-153 | 445 |
| MsG0780039026.01.T01 | MTR_7g066360 | 55.556 | 468 | 181 | 10 | 6 | 462 | 8 | 459 | 2.03e-153 | 446 |
| MsG0780039026.01.T01 | MTR_7g058710 | 48.374 | 492 | 199 | 8 | 1 | 452 | 1 | 477 | 4.01e-139 | 409 |
| MsG0780039026.01.T01 | MTR_7g066580 | 47.303 | 482 | 166 | 11 | 18 | 452 | 1 | 441 | 3.49e-128 | 380 |
| MsG0780039026.01.T01 | MTR_7g066580 | 28.854 | 253 | 139 | 9 | 4 | 233 | 207 | 441 | 3.47e-12 | 68.6 |
| MsG0780039026.01.T01 | MTR_7g066410 | 41.715 | 513 | 225 | 12 | 12 | 456 | 10 | 516 | 9.98e-117 | 353 |
| MsG0780039026.01.T01 | MTR_7g066430 | 42.828 | 488 | 226 | 12 | 12 | 452 | 4 | 485 | 1.22e-115 | 350 |
| MsG0780039026.01.T01 | MTR_7g066430 | 28.205 | 234 | 149 | 6 | 9 | 231 | 254 | 479 | 3.94e-14 | 75.1 |
| MsG0780039026.01.T01 | MTR_7g058700 | 46.532 | 447 | 165 | 7 | 24 | 452 | 531 | 921 | 1.55e-112 | 354 |
| MsG0780039026.01.T01 | MTR_2g029400 | 44.894 | 470 | 150 | 9 | 6 | 440 | 8 | 403 | 7.32e-112 | 337 |
| MsG0780039026.01.T01 | MTR_2g029400 | 30.653 | 199 | 109 | 8 | 255 | 446 | 7 | 183 | 9.05e-11 | 64.3 |
| MsG0780039026.01.T01 | MTR_7g066480 | 41.020 | 451 | 213 | 9 | 11 | 452 | 15 | 421 | 3.17e-98 | 303 |
| MsG0780039026.01.T01 | MTR_7g066490 | 40.583 | 446 | 208 | 9 | 12 | 453 | 16 | 408 | 4.77e-93 | 289 |
| MsG0780039026.01.T01 | MTR_7g062300 | 37.014 | 489 | 253 | 12 | 1 | 451 | 1 | 472 | 3.19e-92 | 288 |
| MsG0780039026.01.T01 | MTR_7g062280 | 36.400 | 500 | 249 | 11 | 5 | 452 | 3 | 485 | 1.00e-91 | 288 |
| MsG0780039026.01.T01 | MTR_7g063900 | 40.447 | 492 | 237 | 14 | 5 | 452 | 3 | 482 | 2.64e-91 | 287 |
| MsG0780039026.01.T01 | MTR_7g063820 | 37.832 | 489 | 255 | 11 | 5 | 454 | 2 | 480 | 8.17e-88 | 278 |
| MsG0780039026.01.T01 | MTR_7g063660 | 36.853 | 483 | 253 | 11 | 7 | 452 | 4 | 471 | 4.01e-87 | 275 |
| MsG0780039026.01.T01 | MTR_7g063880 | 37.267 | 483 | 248 | 13 | 5 | 454 | 4 | 464 | 6.98e-87 | 275 |
| MsG0780039026.01.T01 | MTR_7g063890 | 38.923 | 483 | 244 | 11 | 11 | 452 | 26 | 498 | 1.58e-85 | 272 |
| MsG0780039026.01.T01 | MTR_7g063830 | 38.362 | 464 | 248 | 14 | 1 | 452 | 1 | 438 | 8.86e-85 | 268 |
| MsG0780039026.01.T01 | MTR_7g063840 | 35.404 | 483 | 265 | 12 | 1 | 452 | 1 | 467 | 7.00e-84 | 267 |
| MsG0780039026.01.T01 | MTR_7g063920 | 35.639 | 477 | 235 | 10 | 1 | 452 | 1 | 430 | 1.88e-82 | 262 |
| MsG0780039026.01.T01 | MTR_7g063920 | 31.628 | 215 | 120 | 10 | 259 | 464 | 7 | 203 | 6.32e-17 | 83.2 |
| MsG0780039026.01.T01 | MTR_4g019310 | 34.669 | 499 | 256 | 12 | 6 | 448 | 2 | 486 | 3.30e-82 | 263 |
| MsG0780039026.01.T01 | MTR_3g007740 | 37.849 | 465 | 236 | 15 | 11 | 452 | 8 | 442 | 1.37e-78 | 252 |
| MsG0780039026.01.T01 | MTR_4g017830 | 33.837 | 529 | 273 | 16 | 1 | 466 | 1 | 515 | 3.01e-77 | 251 |
| MsG0780039026.01.T01 | MTR_4g019340 | 34.591 | 477 | 251 | 12 | 13 | 441 | 39 | 502 | 7.61e-75 | 244 |
| MsG0780039026.01.T01 | MTR_4g017440 | 33.006 | 509 | 265 | 13 | 6 | 452 | 8 | 502 | 9.31e-74 | 241 |
| MsG0780039026.01.T01 | MTR_3g065200 | 35.269 | 465 | 209 | 13 | 10 | 439 | 1 | 408 | 4.36e-73 | 238 |
| MsG0780039026.01.T01 | MTR_4g017410 | 33.665 | 502 | 258 | 18 | 6 | 452 | 3 | 484 | 9.75e-71 | 234 |
| MsG0780039026.01.T01 | MTR_1g014060 | 55.760 | 217 | 88 | 4 | 21 | 234 | 9 | 220 | 1.78e-70 | 229 |
| MsG0780039026.01.T01 | MTR_1g014060 | 48.295 | 176 | 75 | 4 | 292 | 452 | 210 | 384 | 1.46e-34 | 134 |
| MsG0780039026.01.T01 | MTR_7g063670 | 35.022 | 454 | 263 | 8 | 13 | 452 | 16 | 451 | 5.84e-70 | 230 |
| MsG0780039026.01.T01 | MTR_7g066680 | 47.157 | 299 | 95 | 5 | 214 | 449 | 37 | 335 | 9.84e-70 | 226 |
| MsG0780039026.01.T01 | MTR_7g066680 | 27.727 | 220 | 139 | 7 | 6 | 217 | 80 | 287 | 8.46e-11 | 63.9 |
| MsG0780039026.01.T01 | MTR_4g017610 | 33.333 | 489 | 244 | 18 | 15 | 449 | 16 | 476 | 2.61e-69 | 229 |
| MsG0780039026.01.T01 | MTR_4g017610 | 28.514 | 249 | 132 | 8 | 255 | 459 | 6 | 252 | 4.92e-14 | 74.7 |
| MsG0780039026.01.T01 | MTR_4g017470 | 30.680 | 515 | 249 | 12 | 1 | 452 | 1 | 470 | 1.86e-60 | 206 |
| MsG0780039026.01.T01 | MTR_4g017550 | 30.818 | 477 | 238 | 18 | 41 | 450 | 20 | 471 | 4.72e-49 | 175 |
| MsG0780039026.01.T01 | MTR_7g066390 | 51.553 | 161 | 50 | 1 | 303 | 463 | 1 | 133 | 2.76e-44 | 153 |
| MsG0780039026.01.T01 | MTR_7g063720 | 35.714 | 210 | 122 | 3 | 256 | 452 | 17 | 226 | 2.60e-35 | 132 |
| MsG0780039026.01.T01 | MTR_7g063720 | 28.448 | 232 | 135 | 9 | 3 | 231 | 17 | 220 | 4.31e-16 | 78.2 |
| MsG0780039026.01.T01 | MTR_7g062270 | 39.181 | 171 | 103 | 1 | 13 | 183 | 11 | 180 | 1.10e-34 | 134 |
| MsG0780039026.01.T01 | MTR_7g062270 | 30.488 | 164 | 80 | 6 | 267 | 398 | 16 | 177 | 3.83e-11 | 65.1 |
| MsG0780039026.01.T01 | MTR_2g067310 | 42.775 | 173 | 59 | 5 | 23 | 195 | 58 | 190 | 1.31e-28 | 114 |
| MsG0780039026.01.T01 | MTR_7g063850 | 30.592 | 304 | 150 | 10 | 185 | 452 | 4 | 282 | 2.87e-27 | 111 |
| MsG0780039026.01.T01 | MTR_7g063850 | 26.522 | 230 | 134 | 9 | 15 | 231 | 69 | 276 | 7.11e-14 | 72.8 |
| MsG0780039026.01.T01 | MTR_7g100420 | 33.133 | 332 | 171 | 11 | 155 | 452 | 83 | 397 | 8.74e-27 | 112 |
| MsG0780039026.01.T01 | MTR_7g063690 | 32.014 | 278 | 120 | 9 | 13 | 289 | 13 | 222 | 4.27e-25 | 103 |
| MsG0780039026.01.T01 | MTR_8g023240 | 24.551 | 501 | 291 | 16 | 1 | 453 | 29 | 490 | 4.39e-25 | 108 |
| MsG0780039026.01.T01 | MTR_1g115440 | 32.411 | 253 | 141 | 8 | 1 | 237 | 1 | 239 | 7.66e-24 | 100 |
| MsG0780039026.01.T01 | MTR_8g445860 | 30.769 | 247 | 145 | 7 | 4 | 237 | 5 | 238 | 8.59e-24 | 100 |
| MsG0780039026.01.T01 | MTR_8g445860 | 29.464 | 224 | 117 | 4 | 260 | 445 | 11 | 231 | 2.56e-21 | 93.2 |
| MsG0780039026.01.T01 | MTR_0150s0010 | 31.600 | 250 | 146 | 8 | 1 | 237 | 1 | 238 | 4.15e-21 | 92.8 |
| MsG0780039026.01.T01 | MTR_0150s0010 | 30.131 | 229 | 112 | 7 | 260 | 445 | 8 | 231 | 1.35e-19 | 88.2 |
| MsG0780039026.01.T01 | MTR_4g019330 | 35.294 | 187 | 89 | 8 | 276 | 459 | 32 | 189 | 8.36e-18 | 82.0 |
| MsG0780039026.01.T01 | MTR_1g115455 | 28.112 | 249 | 144 | 8 | 6 | 237 | 3 | 233 | 1.93e-15 | 76.3 |
| MsG0780039026.01.T01 | MTR_4g019240 | 36.842 | 114 | 60 | 4 | 3 | 108 | 4 | 113 | 6.09e-15 | 73.6 |
| MsG0780039026.01.T01 | MTR_1g115470 | 40.952 | 105 | 54 | 3 | 12 | 114 | 6 | 104 | 1.59e-14 | 75.9 |
| MsG0780039026.01.T01 | MTR_1g115450 | 40.000 | 90 | 49 | 1 | 7 | 96 | 2 | 86 | 4.13e-14 | 69.7 |
| MsG0780039026.01.T01 | MTR_7g063760 | 46.970 | 66 | 34 | 1 | 388 | 452 | 2 | 67 | 4.83e-13 | 64.7 |
| MsG0780039026.01.T01 | MTR_1g115435 | 27.966 | 236 | 139 | 9 | 7 | 238 | 2 | 210 | 2.23e-12 | 67.0 |
| MsG0780039026.01.T01 | MTR_0150s0030 | 36.264 | 91 | 52 | 2 | 6 | 95 | 2 | 87 | 1.89e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039026.01.T01 | AT3G09140 | 32.150 | 507 | 279 | 16 | 1 | 454 | 1 | 495 | 5.32e-62 | 211 |
| MsG0780039026.01.T01 | AT5G01120 | 30.992 | 484 | 274 | 13 | 15 | 448 | 12 | 485 | 1.07e-60 | 207 |
| MsG0780039026.01.T01 | AT3G09140 | 30.394 | 533 | 282 | 16 | 1 | 454 | 1 | 523 | 4.23e-58 | 201 |
| MsG0780039026.01.T01 | AT5G01150 | 31.818 | 484 | 278 | 12 | 12 | 454 | 9 | 481 | 1.76e-57 | 198 |
| MsG0780039026.01.T01 | AT5G43240 | 30.247 | 486 | 279 | 13 | 15 | 448 | 12 | 489 | 2.02e-54 | 191 |
| MsG0780039026.01.T01 | AT5G43240 | 30.247 | 486 | 279 | 13 | 15 | 448 | 12 | 489 | 2.02e-54 | 191 |
| MsG0780039026.01.T01 | AT5G43240 | 30.247 | 486 | 279 | 13 | 15 | 448 | 12 | 489 | 2.02e-54 | 191 |
| MsG0780039026.01.T01 | AT5G01140 | 29.375 | 480 | 283 | 10 | 12 | 462 | 9 | 461 | 2.65e-54 | 189 |
| MsG0780039026.01.T01 | AT5G01130 | 31.197 | 468 | 286 | 10 | 1 | 454 | 1 | 446 | 7.34e-51 | 179 |
| MsG0780039026.01.T01 | AT3G09110 | 32.812 | 320 | 205 | 5 | 12 | 327 | 9 | 322 | 3.13e-49 | 172 |
| MsG0780039026.01.T01 | AT3G09120 | 32.278 | 316 | 197 | 6 | 11 | 316 | 5 | 313 | 1.60e-41 | 151 |
| MsG0780039026.01.T01 | AT3G09120 | 27.126 | 247 | 134 | 9 | 259 | 464 | 3 | 244 | 3.93e-13 | 70.9 |
| MsG0780039026.01.T01 | AT5G37320 | 26.962 | 497 | 301 | 17 | 3 | 454 | 2 | 481 | 3.00e-38 | 146 |
| MsG0780039026.01.T01 | AT5G43240 | 29.141 | 326 | 186 | 10 | 163 | 448 | 25 | 345 | 3.41e-25 | 107 |
Find 72 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGTTCAGTTTCATATTC+TGG | 0.191679 | 7:+56963506 | None:intergenic |
| GATCCTAAATCCCCTATTTC+TGG | 0.192592 | 7:-56962584 | MsG0780039026.01.T01:CDS |
| GTCCGACTTTATATTTAAAA+AGG | 0.208090 | 7:-56963011 | MsG0780039026.01.T01:CDS |
| TGGAGCATCATTAACGTTTA+TGG | 0.208826 | 7:-56962552 | MsG0780039026.01.T01:CDS |
| CCATTTCCTCCACATCATTA+AGG | 0.210010 | 7:+56962456 | None:intergenic |
| TAATCATCTGCTGAGTTTCT+TGG | 0.229674 | 7:+56963532 | None:intergenic |
| CCAGCATATCCACCAGAAAT+AGG | 0.239307 | 7:+56962572 | None:intergenic |
| AAGAAAACATACCTCTTTCT+TGG | 0.258951 | 7:+56963183 | None:intergenic |
| CCACCAGAAATAGGGGATTT+AGG | 0.261981 | 7:+56962581 | None:intergenic |
| CAAAGTTATCATTAGAGTTT+TGG | 0.270973 | 7:-56963782 | MsG0780039026.01.T01:CDS |
| TGATCTACTCAAATTGTCTT+TGG | 0.290186 | 7:-56963050 | MsG0780039026.01.T01:CDS |
| GAGTTCAGATTGAGAGAAAA+TGG | 0.304664 | 7:+56962673 | None:intergenic |
| AACCAATTGGAGGTTAATAT+TGG | 0.305540 | 7:-56962959 | MsG0780039026.01.T01:CDS |
| CTTGGTAATGTTGATATTAA+CGG | 0.313085 | 7:+56963201 | None:intergenic |
| AAACTTGTCGTAACAACATT+TGG | 0.329105 | 7:+56963277 | None:intergenic |
| CGATTTCTTTACTCAAAATC+AGG | 0.337067 | 7:+56963353 | None:intergenic |
| AATACTGTGTAAGTTTCTTC+AGG | 0.340897 | 7:-56963420 | MsG0780039026.01.T01:CDS |
| CAGCATATCCACCAGAAATA+GGG | 0.355977 | 7:+56962573 | None:intergenic |
| GAGGAAATGGTCGTTAATAT+TGG | 0.365896 | 7:-56962443 | MsG0780039026.01.T01:CDS |
| GAGAACGAGACTTGCAGAAT+TGG | 0.376890 | 7:-56963448 | MsG0780039026.01.T01:CDS |
| TTTAGTTCTAAGTAACCAAT+TGG | 0.384220 | 7:-56962972 | MsG0780039026.01.T01:CDS |
| CAAAATCTGCTTTGACAAAC+GGG | 0.394326 | 7:-56962291 | MsG0780039026.01.T01:CDS |
| GCTTCTTAACATTGCGCTTA+GGG | 0.394593 | 7:-56963693 | MsG0780039026.01.T01:CDS |
| CCTATTTCTGGTGGATATGC+TGG | 0.397124 | 7:-56962572 | MsG0780039026.01.T01:CDS |
| CATTTCCTCCACATCATTAA+GGG | 0.397517 | 7:+56962457 | None:intergenic |
| AAAGGAAGAATCTGATGAAA+TGG | 0.399313 | 7:-56962930 | MsG0780039026.01.T01:CDS |
| TTAAATCTCCTTCAGAAGCT+TGG | 0.404186 | 7:-56963256 | MsG0780039026.01.T01:CDS |
| GTTTATTATAATTATAATGT+AGG | 0.409424 | 7:+56962219 | None:intergenic |
| GTTTATGGTGACAGACGAAT+TGG | 0.414631 | 7:-56962537 | MsG0780039026.01.T01:CDS |
| TACTATACTTACCAATGAAC+TGG | 0.417203 | 7:+56962263 | None:intergenic |
| TCAAAATCTGCTTTGACAAA+CGG | 0.417718 | 7:-56962292 | MsG0780039026.01.T01:CDS |
| AGCTTCTTAACATTGCGCTT+AGG | 0.417921 | 7:-56963694 | MsG0780039026.01.T01:CDS |
| TCAAAATCAGGAATTCTTCT+CGG | 0.418915 | 7:+56963365 | None:intergenic |
| AATATTGAAGCACTGAGCTT+TGG | 0.420564 | 7:-56963640 | MsG0780039026.01.T01:CDS |
| TACCAATATTAACCTCCAAT+TGG | 0.431128 | 7:+56962957 | None:intergenic |
| AAGATATGAAATACCATCAA+TGG | 0.434003 | 7:+56962496 | None:intergenic |
| CCTTAATGATGTGGAGGAAA+TGG | 0.434219 | 7:-56962456 | MsG0780039026.01.T01:CDS |
| AACGGGCTCAACCAGTTCAT+TGG | 0.445458 | 7:-56962274 | MsG0780039026.01.T01:CDS |
| ATGTAGGACAAGGTTATGTT+TGG | 0.456947 | 7:+56962235 | None:intergenic |
| TAACATAAAGATCATCAGAT+AGG | 0.479696 | 7:+56963302 | None:intergenic |
| TTCATATCTTGAAAGAATGA+AGG | 0.484244 | 7:-56962483 | MsG0780039026.01.T01:CDS |
| GGTTAATATTGGTAAAGAAA+AGG | 0.492157 | 7:-56962948 | MsG0780039026.01.T01:CDS |
| AGGAATCAGAAATGTATTTG+TGG | 0.500975 | 7:-56963400 | MsG0780039026.01.T01:CDS |
| TCAGATTGAGAGAAAATGGT+TGG | 0.508160 | 7:+56962677 | None:intergenic |
| TGAGTTCAGACAAGTGTTTG+AGG | 0.508556 | 7:-56962730 | MsG0780039026.01.T01:CDS |
| AGCTTACGAAAGAGAATCCT+TGG | 0.509949 | 7:+56962786 | None:intergenic |
| TTGAGAGAAAATGGTTGGAC+TGG | 0.513525 | 7:+56962682 | None:intergenic |
| TATCAACATTACCAAGAAAG+AGG | 0.515400 | 7:-56963194 | MsG0780039026.01.T01:intron |
| AGTTCTAAGTAACCAATTGG+AGG | 0.534683 | 7:-56962969 | MsG0780039026.01.T01:CDS |
| TCTATTTGCTGAGGCAGAGA+AGG | 0.537242 | 7:-56963737 | MsG0780039026.01.T01:CDS |
| AATACGCAACACTGCTCCCA+AGG | 0.546593 | 7:+56962811 | None:intergenic |
| ACTCCAATGTCATCCATTGA+TGG | 0.546893 | 7:-56962509 | MsG0780039026.01.T01:CDS |
| AAAATCAGCAAAATCTTCCT+CGG | 0.555219 | 7:+56962856 | None:intergenic |
| CATTGACTCCAAGCTTCTGA+AGG | 0.561296 | 7:+56963248 | None:intergenic |
| AGCATATCCACCAGAAATAG+GGG | 0.561344 | 7:+56962574 | None:intergenic |
| TATAATTATAATGTAGGACA+AGG | 0.575132 | 7:+56962225 | None:intergenic |
| CCTAAATCCCCTATTTCTGG+TGG | 0.575624 | 7:-56962581 | MsG0780039026.01.T01:CDS |
| CTGGTTAGTATGTCTTTGAA+AGG | 0.575831 | 7:+56962701 | None:intergenic |
| CTCGTTCTCACAGCAGAAGT+AGG | 0.577883 | 7:+56963462 | None:intergenic |
| ATTTCCTCCACATCATTAAG+GGG | 0.596639 | 7:+56962458 | None:intergenic |
| GCAGTGTTGCGTATTCTCCA+AGG | 0.600046 | 7:-56962803 | MsG0780039026.01.T01:CDS |
| AAATAAAGTTCTATTTGCTG+AGG | 0.602370 | 7:-56963746 | MsG0780039026.01.T01:CDS |
| AGGGACCATTGCAAAACTTG+TGG | 0.604088 | 7:-56963674 | MsG0780039026.01.T01:CDS |
| CGTTAATATTGGCCAGAAGG+AGG | 0.604404 | 7:-56962432 | MsG0780039026.01.T01:intron |
| ATACCATCAATGGATGACAT+TGG | 0.606080 | 7:+56962506 | None:intergenic |
| GGTCGTTAATATTGGCCAGA+AGG | 0.607810 | 7:-56962435 | MsG0780039026.01.T01:CDS |
| ACAGCAGAAGTAGGACTGCA+AGG | 0.621696 | 7:+56963471 | None:intergenic |
| ATACGCAACACTGCTCCCAA+GGG | 0.634798 | 7:+56962812 | None:intergenic |
| TTAAATATAAAGTCGGACAG+AGG | 0.641473 | 7:+56963016 | None:intergenic |
| TTTCCTCCACATCATTAAGG+GGG | 0.685271 | 7:+56962459 | None:intergenic |
| TACGCAACACTGCTCCCAAG+GGG | 0.692051 | 7:+56962813 | None:intergenic |
| TCTATGGTCACAGACATGCA+AGG | 0.694458 | 7:-56963569 | MsG0780039026.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTATTATAATTATAATGT+AGG | + | Chr7:56963760-56963779 | None:intergenic | 10.0% |
| !! | AATACCTAAAAACAATTATA+AGG | - | Chr7:56963343-56963362 | MsG0780039026.01.T01:CDS | 15.0% |
| !!! | TTATAATTGTTTTTAGGTAT+TGG | + | Chr7:56963344-56963363 | None:intergenic | 15.0% |
| !!! | TTCCTTTTTAAATATAAAGT+CGG | + | Chr7:56962970-56962989 | None:intergenic | 15.0% |
| !!! | TTTTCCTTATAATTGTTTTT+AGG | + | Chr7:56963350-56963369 | None:intergenic | 15.0% |
| !! | ATCTTGATGAAAAATATCTA+TGG | - | Chr7:56962391-56962410 | MsG0780039026.01.T01:intron | 20.0% |
| !! | TATAATTATAATGTAGGACA+AGG | + | Chr7:56963754-56963773 | None:intergenic | 20.0% |
| ! | AAGATATGAAATACCATCAA+TGG | + | Chr7:56963483-56963502 | None:intergenic | 25.0% |
| ! | GGTTAATATTGGTAAAGAAA+AGG | - | Chr7:56963028-56963047 | MsG0780039026.01.T01:CDS | 25.0% |
| ! | GTCCGACTTTATATTTAAAA+AGG | - | Chr7:56962965-56962984 | MsG0780039026.01.T01:CDS | 25.0% |
| ! | TAACATAAAGATCATCAGAT+AGG | + | Chr7:56962677-56962696 | None:intergenic | 25.0% |
| ! | TTCATATCTTGAAAGAATGA+AGG | - | Chr7:56963493-56963512 | MsG0780039026.01.T01:CDS | 25.0% |
| !! | AAATAAAGTTCTATTTGCTG+AGG | - | Chr7:56962230-56962249 | MsG0780039026.01.T01:CDS | 25.0% |
| !! | CAAAGTTATCATTAGAGTTT+TGG | - | Chr7:56962194-56962213 | MsG0780039026.01.T01:CDS | 25.0% |
| !! | TCACTATATTTGATTTCATG+TGG | - | Chr7:56963625-56963644 | MsG0780039026.01.T01:CDS | 25.0% |
| !!! | CTGATTTTGAGTAAAGAAAT+CGG | - | Chr7:56962624-56962643 | MsG0780039026.01.T01:CDS | 25.0% |
| !!! | CTTGGTAATGTTGATATTAA+CGG | + | Chr7:56962778-56962797 | None:intergenic | 25.0% |
| !!! | TTTAGTTCTAAGTAACCAAT+TGG | - | Chr7:56963004-56963023 | MsG0780039026.01.T01:CDS | 25.0% |
| !!! | TTTTAGGTATTGGTAATATC+TGG | + | Chr7:56963334-56963353 | None:intergenic | 25.0% |
| AAAATCAGCAAAATCTTCCT+CGG | + | Chr7:56963123-56963142 | None:intergenic | 30.0% | |
| AAACTTGTCGTAACAACATT+TGG | + | Chr7:56962702-56962721 | None:intergenic | 30.0% | |
| AAAGGAAGAATCTGATGAAA+TGG | - | Chr7:56963046-56963065 | MsG0780039026.01.T01:CDS | 30.0% | |
| AACCAATTGGAGGTTAATAT+TGG | - | Chr7:56963017-56963036 | MsG0780039026.01.T01:CDS | 30.0% | |
| AAGAAAACATACCTCTTTCT+TGG | + | Chr7:56962796-56962815 | None:intergenic | 30.0% | |
| AATACTGTGTAAGTTTCTTC+AGG | - | Chr7:56962556-56962575 | MsG0780039026.01.T01:CDS | 30.0% | |
| AGGAATCAGAAATGTATTTG+TGG | - | Chr7:56962576-56962595 | MsG0780039026.01.T01:CDS | 30.0% | |
| CAATGTTCAGTTTCATATTC+TGG | + | Chr7:56962473-56962492 | None:intergenic | 30.0% | |
| CGATTTCTTTACTCAAAATC+AGG | + | Chr7:56962626-56962645 | None:intergenic | 30.0% | |
| CTCACTAATTCTTCAATATG+TGG | - | Chr7:56962889-56962908 | MsG0780039026.01.T01:CDS | 30.0% | |
| CTTCAATATGTGGTTTGAAT+AGG | - | Chr7:56962899-56962918 | MsG0780039026.01.T01:CDS | 30.0% | |
| TACCAATATTAACCTCCAAT+TGG | + | Chr7:56963022-56963041 | None:intergenic | 30.0% | |
| TACTATACTTACCAATGAAC+TGG | + | Chr7:56963716-56963735 | None:intergenic | 30.0% | |
| TATCAACATTACCAAGAAAG+AGG | - | Chr7:56962782-56962801 | MsG0780039026.01.T01:CDS | 30.0% | |
| TCAAAATCAGGAATTCTTCT+CGG | + | Chr7:56962614-56962633 | None:intergenic | 30.0% | |
| TCAAAATCTGCTTTGACAAA+CGG | - | Chr7:56963684-56963703 | MsG0780039026.01.T01:CDS | 30.0% | |
| TGATCTACTCAAATTGTCTT+TGG | - | Chr7:56962926-56962945 | MsG0780039026.01.T01:CDS | 30.0% | |
| TTAAATATAAAGTCGGACAG+AGG | + | Chr7:56962963-56962982 | None:intergenic | 30.0% | |
| !! | AGTTATCATTAGAGTTTTGG+TGG | - | Chr7:56962197-56962216 | MsG0780039026.01.T01:CDS | 30.0% |
| !!! | TAAAGAAATCGGTTTTGTTC+AGG | - | Chr7:56962635-56962654 | MsG0780039026.01.T01:CDS | 30.0% |
| ATACCATCAATGGATGACAT+TGG | + | Chr7:56963473-56963492 | None:intergenic | 35.0% | |
| ATGTAGGACAAGGTTATGTT+TGG | + | Chr7:56963744-56963763 | None:intergenic | 35.0% | |
| ATTTCCTCCACATCATTAAG+GGG | + | Chr7:56963521-56963540 | None:intergenic | 35.0% | |
| CAAAATCTGCTTTGACAAAC+GGG | - | Chr7:56963685-56963704 | MsG0780039026.01.T01:CDS | 35.0% | |
| CATTTCCTCCACATCATTAA+GGG | + | Chr7:56963522-56963541 | None:intergenic | 35.0% | |
| CTGGTTAGTATGTCTTTGAA+AGG | + | Chr7:56963278-56963297 | None:intergenic | 35.0% | |
| GAGGAAATGGTCGTTAATAT+TGG | - | Chr7:56963533-56963552 | MsG0780039026.01.T01:CDS | 35.0% | |
| GAGTTCAGATTGAGAGAAAA+TGG | + | Chr7:56963306-56963325 | None:intergenic | 35.0% | |
| TAATCATCTGCTGAGTTTCT+TGG | + | Chr7:56962447-56962466 | None:intergenic | 35.0% | |
| TCAGATTGAGAGAAAATGGT+TGG | + | Chr7:56963302-56963321 | None:intergenic | 35.0% | |
| TTAAATCTCCTTCAGAAGCT+TGG | - | Chr7:56962720-56962739 | MsG0780039026.01.T01:CDS | 35.0% | |
| ! | ATTAGCCACAAGTTTTGCAA+TGG | + | Chr7:56962310-56962329 | None:intergenic | 35.0% |
| ! | ATTTGATTTCATGTGGTTGC+AGG | - | Chr7:56963632-56963651 | MsG0780039026.01.T01:CDS | 35.0% |
| ! | GTTTGTCAAAGCAGATTTTG+AGG | + | Chr7:56963685-56963704 | None:intergenic | 35.0% |
| ! | TGGAGCATCATTAACGTTTA+TGG | - | Chr7:56963424-56963443 | MsG0780039026.01.T01:CDS | 35.0% |
| ! | TTTGATTTCATGTGGTTGCA+GGG | - | Chr7:56963633-56963652 | MsG0780039026.01.T01:CDS | 35.0% |
| ! | TTTTCTCTCAATCTGAACTC+AGG | - | Chr7:56963306-56963325 | MsG0780039026.01.T01:CDS | 35.0% |
| !! | AATATTGAAGCACTGAGCTT+TGG | - | Chr7:56962336-56962355 | MsG0780039026.01.T01:intron | 35.0% |
| !! | AGTTCTAAGTAACCAATTGG+AGG | - | Chr7:56963007-56963026 | MsG0780039026.01.T01:CDS | 35.0% |
| !!! | TTTGTCTACTTGTTTTGCTG+AGG | + | Chr7:56962178-56962197 | None:intergenic | 35.0% |
| ACTCCAATGTCATCCATTGA+TGG | - | Chr7:56963467-56963486 | MsG0780039026.01.T01:CDS | 40.0% | |
| AGCATATCCACCAGAAATAG+GGG | + | Chr7:56963405-56963424 | None:intergenic | 40.0% | |
| AGCTTACGAAAGAGAATCCT+TGG | + | Chr7:56963193-56963212 | None:intergenic | 40.0% | |
| AGCTTCTTAACATTGCGCTT+AGG | - | Chr7:56962282-56962301 | MsG0780039026.01.T01:CDS | 40.0% | |
| CAGCATATCCACCAGAAATA+GGG | + | Chr7:56963406-56963425 | None:intergenic | 40.0% | |
| CCATTTCCTCCACATCATTA+AGG | + | Chr7:56963523-56963542 | None:intergenic | 40.0% | |
| CCTTAATGATGTGGAGGAAA+TGG | - | Chr7:56963520-56963539 | MsG0780039026.01.T01:CDS | 40.0% | |
| GATCCTAAATCCCCTATTTC+TGG | - | Chr7:56963392-56963411 | MsG0780039026.01.T01:CDS | 40.0% | |
| GCTTCTTAACATTGCGCTTA+GGG | - | Chr7:56962283-56962302 | MsG0780039026.01.T01:CDS | 40.0% | |
| GTTTATGGTGACAGACGAAT+TGG | - | Chr7:56963439-56963458 | MsG0780039026.01.T01:CDS | 40.0% | |
| TGAGTTCAGACAAGTGTTTG+AGG | - | Chr7:56963246-56963265 | MsG0780039026.01.T01:CDS | 40.0% | |
| TTGAGAGAAAATGGTTGGAC+TGG | + | Chr7:56963297-56963316 | None:intergenic | 40.0% | |
| TTTCCTCCACATCATTAAGG+GGG | + | Chr7:56963520-56963539 | None:intergenic | 40.0% | |
| ! | AGTTTTCTCACTTTCCCCTT+GGG | - | Chr7:56963149-56963168 | MsG0780039026.01.T01:intron | 40.0% |
| ! | TAGTTTTCTCACTTTCCCCT+TGG | - | Chr7:56963148-56963167 | MsG0780039026.01.T01:intron | 40.0% |
| !! | GATTTTGTTTGTTGAAGCCG+AGG | - | Chr7:56963103-56963122 | MsG0780039026.01.T01:intron | 40.0% |
| AGGGACCATTGCAAAACTTG+TGG | - | Chr7:56962302-56962321 | MsG0780039026.01.T01:CDS | 45.0% | |
| ATTGGCCAGAAGGAGGTAAA+AGG | - | Chr7:56963551-56963570 | MsG0780039026.01.T01:CDS | 45.0% | |
| CCACCAGAAATAGGGGATTT+AGG | + | Chr7:56963398-56963417 | None:intergenic | 45.0% | |
| CCAGCATATCCACCAGAAAT+AGG | + | Chr7:56963407-56963426 | None:intergenic | 45.0% | |
| CGTTAATATTGGCCAGAAGG+AGG | - | Chr7:56963544-56963563 | MsG0780039026.01.T01:CDS | 45.0% | |
| GAGAACGAGACTTGCAGAAT+TGG | - | Chr7:56962528-56962547 | MsG0780039026.01.T01:CDS | 45.0% | |
| GGTCGTTAATATTGGCCAGA+AGG | - | Chr7:56963541-56963560 | MsG0780039026.01.T01:CDS | 45.0% | |
| TCTATGGTCACAGACATGCA+AGG | - | Chr7:56962407-56962426 | MsG0780039026.01.T01:intron | 45.0% | |
| ! | CACTTCCTTTTACCTCCTTC+TGG | + | Chr7:56963559-56963578 | None:intergenic | 45.0% |
| ! | TCTATTTGCTGAGGCAGAGA+AGG | - | Chr7:56962239-56962258 | MsG0780039026.01.T01:CDS | 45.0% |
| !! | CATTGACTCCAAGCTTCTGA+AGG | + | Chr7:56962731-56962750 | None:intergenic | 45.0% |
| !! | CCTAAATCCCCTATTTCTGG+TGG | - | Chr7:56963395-56963414 | MsG0780039026.01.T01:CDS | 45.0% |
| !! | CCTATTTCTGGTGGATATGC+TGG | - | Chr7:56963404-56963423 | MsG0780039026.01.T01:CDS | 45.0% |
| AATACGCAACACTGCTCCCA+AGG | + | Chr7:56963168-56963187 | None:intergenic | 50.0% | |
| ACAGCAGAAGTAGGACTGCA+AGG | + | Chr7:56962508-56962527 | None:intergenic | 50.0% | |
| ATACGCAACACTGCTCCCAA+GGG | + | Chr7:56963167-56963186 | None:intergenic | 50.0% | |
| CTCGTTCTCACAGCAGAAGT+AGG | + | Chr7:56962517-56962536 | None:intergenic | 50.0% | |
| GAAGGTCCCCCTTAATGATG+TGG | - | Chr7:56963511-56963530 | MsG0780039026.01.T01:CDS | 50.0% | |
| GCAGTGTTGCGTATTCTCCA+AGG | - | Chr7:56963173-56963192 | MsG0780039026.01.T01:intron | 50.0% | |
| ! | AACGGGCTCAACCAGTTCAT+TGG | - | Chr7:56963702-56963721 | MsG0780039026.01.T01:CDS | 50.0% |
| GGTCCCCCTTAATGATGTGG+AGG | - | Chr7:56963514-56963533 | MsG0780039026.01.T01:CDS | 55.0% | |
| TACGCAACACTGCTCCCAAG+GGG | + | Chr7:56963166-56963185 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 56962162 | 56963836 | 56962162 | ID=MsG0780039026.01;Name=MsG0780039026.01 |
| Chr7 | mRNA | 56962162 | 56963836 | 56962162 | ID=MsG0780039026.01.T01;Parent=MsG0780039026.01;Name=MsG0780039026.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0|1|1|1|3|0|489 |
| Chr7 | exon | 56963195 | 56963836 | 56963195 | ID=MsG0780039026.01.T01:exon:8205;Parent=MsG0780039026.01.T01 |
| Chr7 | exon | 56962433 | 56963077 | 56962433 | ID=MsG0780039026.01.T01:exon:8204;Parent=MsG0780039026.01.T01 |
| Chr7 | exon | 56962162 | 56962344 | 56962162 | ID=MsG0780039026.01.T01:exon:8203;Parent=MsG0780039026.01.T01 |
| Chr7 | CDS | 56963195 | 56963836 | 56963195 | ID=MsG0780039026.01.T01:cds;Parent=MsG0780039026.01.T01 |
| Chr7 | CDS | 56962433 | 56963077 | 56962433 | ID=MsG0780039026.01.T01:cds;Parent=MsG0780039026.01.T01 |
| Chr7 | CDS | 56962162 | 56962344 | 56962162 | ID=MsG0780039026.01.T01:cds;Parent=MsG0780039026.01.T01 |
| Gene Sequence |
| Protein sequence |
