AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008180.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008180.01.T01 MTR_0062s0090 95.286 297 14 0 1 297 1 297 0.0 588
MsG0280008180.01.T01 MTR_0062s0040 95.286 297 14 0 1 297 1 297 0.0 588
MsG0280008180.01.T01 MTR_0062s0060 95.286 297 14 0 1 297 1 297 0.0 588
MsG0280008180.01.T01 MTR_0062s0020 63.333 300 104 4 1 296 1 298 5.33e-135 384
MsG0280008180.01.T01 MTR_0443s0040 45.246 305 143 10 3 295 15 307 1.79e-75 233
MsG0280008180.01.T01 MTR_0002s1060 45.973 298 139 10 4 295 16 297 4.54e-71 222
MsG0280008180.01.T01 MTR_0062s0170 42.475 299 158 6 4 295 15 306 7.48e-69 216
MsG0280008180.01.T01 MTR_0062s0110 42.475 299 158 6 4 295 15 306 7.48e-69 216
MsG0280008180.01.T01 MTR_0062s0130 42.475 299 158 6 4 295 15 306 7.48e-69 216
MsG0280008180.01.T01 MTR_2g036240 42.475 299 158 6 4 295 15 306 7.48e-69 216
MsG0280008180.01.T01 MTR_2g036260 42.475 299 158 6 4 295 15 306 7.48e-69 216
MsG0280008180.01.T01 MTR_2g036290 42.475 299 158 6 4 295 15 306 7.48e-69 216
MsG0280008180.01.T01 MTR_8g046730 42.475 299 158 6 4 295 15 306 7.48e-69 216
MsG0280008180.01.T01 MTR_0002s0040 42.808 292 151 7 4 295 15 290 1.97e-68 215
MsG0280008180.01.T01 MTR_0263s0020 43.345 293 144 8 4 293 15 288 6.99e-67 211
MsG0280008180.01.T01 MTR_1457s0010 41.284 218 108 8 58 273 1 200 1.54e-42 145
MsG0280008180.01.T01 MTR_2g096030 38.078 281 142 14 2 274 29 285 5.75e-42 147
MsG0280008180.01.T01 MTR_6g079630 35.842 279 147 13 4 274 34 288 7.17e-38 136
MsG0280008180.01.T01 MTR_2g096040 32.841 271 158 11 1 266 1 252 1.71e-25 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGAATTCCACTTCATTT+TGG 0.093829 2:+26799331 MsG0280008180.01.T01:CDS
TTCAAATTTCTCACTGCTTC+TGG 0.128250 2:-26799434 None:intergenic
AGCCATTCTCAACAAATTTA+AGG 0.166592 2:+26799276 MsG0280008180.01.T01:CDS
AAAATGAGGCCAATATAAAT+TGG 0.218265 2:+26799801 MsG0280008180.01.T01:CDS
GAGAATGTCTAAAATCTTTC+GGG 0.256008 2:+26799238 None:intergenic
AACGAAAGGTTTATTCTCAT+TGG 0.271867 2:-26800077 None:intergenic
CTTATTACCACCTTCACATT+TGG 0.282214 2:-26799467 None:intergenic
TGAGAATGTCTAAAATCTTT+CGG 0.303326 2:+26799237 None:intergenic
GAAGTGGAATTCTGAGATAT+TGG 0.303944 2:-26799324 None:intergenic
GTAGTTAACCCAATTTATAT+TGG 0.311688 2:-26799810 None:intergenic
TCCCTTAAATTTGTTGAGAA+TGG 0.328006 2:-26799278 None:intergenic
CCAAGGGAGCACTTCATTGC+TGG 0.335528 2:+26799977 MsG0280008180.01.T01:CDS
GCCATTCTCAACAAATTTAA+GGG 0.342684 2:+26799277 MsG0280008180.01.T01:CDS
TACCAGGGAGTGATGCTATC+TGG 0.345229 2:-26800015 None:intergenic
AATGCCATCAATTATGTTGC+CGG 0.364218 2:-26799603 None:intergenic
ATCCAGATAGTTTCCTCAAC+AGG 0.369815 2:-26799527 None:intergenic
ATCCTGTTGAGGAAACTATC+TGG 0.376187 2:+26799525 MsG0280008180.01.T01:CDS
AAACCTAGTGTAGTCCTTCC+AGG 0.379308 2:+26799911 MsG0280008180.01.T01:CDS
GTTTAGATTGGTTGTAGAAC+TGG 0.408788 2:-26799832 None:intergenic
GACCTGTGTCACCTGTAGTA+TGG 0.420193 2:-26799649 None:intergenic
TCCCTTGGCATCTTGTCTAC+AGG 0.422781 2:-26799962 None:intergenic
ACGAAAGGTTTATTCTCATT+GGG 0.427989 2:-26800076 None:intergenic
TCCACTACCGCAACTTGTAT+TGG 0.430990 2:+26799777 MsG0280008180.01.T01:CDS
AGCTTGTCGGTGCTAACTCC+TGG 0.441987 2:-26799929 None:intergenic
GCCAATACAAGTTGCGGTAG+TGG 0.444051 2:-26799778 None:intergenic
CCAGCAATGAAGTGCTCCCT+TGG 0.447592 2:-26799977 None:intergenic
GTTTCCTCAACAGGATTGAA+TGG 0.450198 2:-26799518 None:intergenic
AAATGAGGCCAATATAAATT+GGG 0.460346 2:+26799802 MsG0280008180.01.T01:CDS
GTAGTGGATTTCATTGGTCT+CGG 0.464555 2:-26799762 None:intergenic
GTGTGGATACAGGTTTAGAT+TGG 0.466973 2:-26799844 None:intergenic
GTTGCGGTAGTGGATTTCAT+TGG 0.476031 2:-26799768 None:intergenic
CAAACCGGCAACATAATTGA+TGG 0.487574 2:+26799599 MsG0280008180.01.T01:CDS
GTTTGTAGTTTCTTGATACC+TGG 0.496915 2:-26799892 None:intergenic
ACTTGGAATACTAGAGCATT+CGG 0.502901 2:+26799410 MsG0280008180.01.T01:CDS
AGAAGCAGTGAGAAATTTGA+AGG 0.503117 2:+26799436 MsG0280008180.01.T01:CDS
AAGTCGTCAAGTGTGGATAC+AGG 0.506124 2:-26799854 None:intergenic
GGTTTATTCTCATTGGGTAG+AGG 0.508257 2:-26800070 None:intergenic
AGGCTCAATGTGAAGCTTGT+CGG 0.519403 2:-26799942 None:intergenic
TGGATTTCATTGGTCTCGGA+TGG 0.520530 2:-26799758 None:intergenic
GAGTATGACGCTCAAAATAG+AGG 0.525674 2:+26799368 MsG0280008180.01.T01:CDS
CTTAACGTCTTCCATACTAC+AGG 0.526898 2:+26799638 MsG0280008180.01.T01:CDS
TATAACTTGCCCTATGCACC+TGG 0.529459 2:-26799684 None:intergenic
GTGAAGGTGGTAATAAGCAT+TGG 0.530420 2:+26799473 MsG0280008180.01.T01:CDS
GACGCTCAAAATAGAGGCAC+AGG 0.531681 2:+26799374 MsG0280008180.01.T01:CDS
TATCTGGATTACGCGGGCTG+TGG 0.534790 2:+26799541 MsG0280008180.01.T01:CDS
AACTCCATTCAATCCTGTTG+AGG 0.541015 2:+26799514 MsG0280008180.01.T01:CDS
ACTCACGAGATTAACTTATG+AGG 0.542547 2:-26800123 None:intergenic
CTCCAGATAGCATCACTCCC+TGG 0.543202 2:+26800013 MsG0280008180.01.T01:CDS
AAAATCTTTCGGGAATACAT+TGG 0.558429 2:+26799248 MsG0280008180.01.T01:CDS
GGGAAATAATCCAAATGTGA+AGG 0.562461 2:+26799457 MsG0280008180.01.T01:CDS
AAGATATCCTCTAAAACGAA+AGG 0.564294 2:-26800091 None:intergenic
ATTGGTCTCGGATGGAGCAA+TGG 0.568409 2:-26799750 None:intergenic
CAACTTGTATTGGCAAAATG+AGG 0.568523 2:+26799787 MsG0280008180.01.T01:CDS
CACGAGATTAACTTATGAGG+AGG 0.580594 2:-26800120 None:intergenic
CTTTCTAAAACACTATGACC+AGG 0.587302 2:+26799874 MsG0280008180.01.T01:CDS
TGGCAAAGCCCAAAATGAAG+TGG 0.590379 2:-26799340 None:intergenic
AAGGTGGTAATAAGCATTGG+AGG 0.601267 2:+26799476 MsG0280008180.01.T01:CDS
AGCCTGTAGACAAGATGCCA+AGG 0.602389 2:+26799960 MsG0280008180.01.T01:CDS
AAATAATCCAAATGTGAAGG+TGG 0.620554 2:+26799460 MsG0280008180.01.T01:CDS
TGTCGGTGCTAACTCCTGGA+AGG 0.636397 2:-26799925 None:intergenic
TTCCATACTACAGGTGACAC+AGG 0.642642 2:+26799647 MsG0280008180.01.T01:CDS
ACTCCTGGAAGGACTACACT+AGG 0.646709 2:-26799914 None:intergenic
AGGAAACTATCTGGATTACG+CGG 0.653571 2:+26799534 MsG0280008180.01.T01:CDS
CTATGCACCTGGCAAAACGA+AGG 0.654236 2:-26799673 None:intergenic
GCCTGTAGACAAGATGCCAA+GGG 0.657771 2:+26799961 MsG0280008180.01.T01:CDS
GAAGCAGTGAGAAATTTGAA+GGG 0.658608 2:+26799437 MsG0280008180.01.T01:CDS
GGAAACTATCTGGATTACGC+GGG 0.674836 2:+26799535 MsG0280008180.01.T01:CDS
CAGGGAGTGATGCTATCTGG+AGG 0.684672 2:-26800012 None:intergenic
TTGAGCGTCATACTCCTCAG+TGG 0.713125 2:-26799360 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATGAGGCCAATATAAAT+TGG + Chr2:26799801-26799820 MsG0280008180.01.T01:CDS 25.0%
! AAATGAGGCCAATATAAATT+GGG + Chr2:26799802-26799821 MsG0280008180.01.T01:CDS 25.0%
! GTAGTTAACCCAATTTATAT+TGG - Chr2:26799813-26799832 None:intergenic 25.0%
!!! TTGGTTTTGATTATTTGAAC+TGG - Chr2:26799308-26799327 None:intergenic 25.0%
AAAATCTTTCGGGAATACAT+TGG + Chr2:26799248-26799267 MsG0280008180.01.T01:CDS 30.0%
AAATAATCCAAATGTGAAGG+TGG + Chr2:26799460-26799479 MsG0280008180.01.T01:CDS 30.0%
AACGAAAGGTTTATTCTCAT+TGG - Chr2:26800080-26800099 None:intergenic 30.0%
ACGAAAGGTTTATTCTCATT+GGG - Chr2:26800079-26800098 None:intergenic 30.0%
AGCCATTCTCAACAAATTTA+AGG + Chr2:26799276-26799295 MsG0280008180.01.T01:CDS 30.0%
AGTGATCATCCAAAAATACA+AGG + Chr2:26799574-26799593 MsG0280008180.01.T01:CDS 30.0%
CAAAAATACAAGGATCAAAC+CGG + Chr2:26799584-26799603 MsG0280008180.01.T01:CDS 30.0%
GCCATTCTCAACAAATTTAA+GGG + Chr2:26799277-26799296 MsG0280008180.01.T01:CDS 30.0%
! GAATAAACCTTTCGTTTTAG+AGG + Chr2:26800084-26800103 MsG0280008180.01.T01:CDS 30.0%
! TCAGAATTCCACTTCATTTT+GGG + Chr2:26799332-26799351 MsG0280008180.01.T01:CDS 30.0%
!! AAGATATCCTCTAAAACGAA+AGG - Chr2:26800094-26800113 None:intergenic 30.0%
!! AGGATTGAATGGAGTTTTAA+CGG - Chr2:26799510-26799529 None:intergenic 30.0%
!! TCCCTTAAATTTGTTGAGAA+TGG - Chr2:26799281-26799300 None:intergenic 30.0%
AATGCCATCAATTATGTTGC+CGG - Chr2:26799606-26799625 None:intergenic 35.0%
AGAAGCAGTGAGAAATTTGA+AGG + Chr2:26799436-26799455 MsG0280008180.01.T01:CDS 35.0%
CGTTCCAAAGAAAAATACCA+GGG - Chr2:26800033-26800052 None:intergenic 35.0%
CTTATTACCACCTTCACATT+TGG - Chr2:26799470-26799489 None:intergenic 35.0%
GAAGCAGTGAGAAATTTGAA+GGG + Chr2:26799437-26799456 MsG0280008180.01.T01:CDS 35.0%
GGGAAATAATCCAAATGTGA+AGG + Chr2:26799457-26799476 MsG0280008180.01.T01:CDS 35.0%
GTTTGTAGTTTCTTGATACC+TGG - Chr2:26799895-26799914 None:intergenic 35.0%
TTCAAATTTCTCACTGCTTC+TGG - Chr2:26799437-26799456 None:intergenic 35.0%
! ACTTGGAATACTAGAGCATT+CGG + Chr2:26799410-26799429 MsG0280008180.01.T01:CDS 35.0%
! CAACTTGTATTGGCAAAATG+AGG + Chr2:26799787-26799806 MsG0280008180.01.T01:CDS 35.0%
! CATTTTGCCAATACAAGTTG+CGG - Chr2:26799787-26799806 None:intergenic 35.0%
! CGGTTTGATCCTTGTATTTT+TGG - Chr2:26799586-26799605 None:intergenic 35.0%
! CTCAGAATTCCACTTCATTT+TGG + Chr2:26799331-26799350 MsG0280008180.01.T01:CDS 35.0%
! GTTTAGATTGGTTGTAGAAC+TGG - Chr2:26799835-26799854 None:intergenic 35.0%
!! CTTTCTAAAACACTATGACC+AGG + Chr2:26799874-26799893 MsG0280008180.01.T01:CDS 35.0%
!! GAAGTGGAATTCTGAGATAT+TGG - Chr2:26799327-26799346 None:intergenic 35.0%
!! TTTTAGAAAGTCGTCAAGTG+TGG - Chr2:26799864-26799883 None:intergenic 35.0%
AACTCCATTCAATCCTGTTG+AGG + Chr2:26799514-26799533 MsG0280008180.01.T01:CDS 40.0%
AGGAAACTATCTGGATTACG+CGG + Chr2:26799534-26799553 MsG0280008180.01.T01:CDS 40.0%
ATCCAGATAGTTTCCTCAAC+AGG - Chr2:26799530-26799549 None:intergenic 40.0%
ATCCTGTTGAGGAAACTATC+TGG + Chr2:26799525-26799544 MsG0280008180.01.T01:CDS 40.0%
CAAACCGGCAACATAATTGA+TGG + Chr2:26799599-26799618 MsG0280008180.01.T01:CDS 40.0%
CTTAACGTCTTCCATACTAC+AGG + Chr2:26799638-26799657 MsG0280008180.01.T01:CDS 40.0%
GAGTATGACGCTCAAAATAG+AGG + Chr2:26799368-26799387 MsG0280008180.01.T01:CDS 40.0%
GCGTTCCAAAGAAAAATACC+AGG - Chr2:26800034-26800053 None:intergenic 40.0%
GGTTTATTCTCATTGGGTAG+AGG - Chr2:26800073-26800092 None:intergenic 40.0%
GTGAAGGTGGTAATAAGCAT+TGG + Chr2:26799473-26799492 MsG0280008180.01.T01:CDS 40.0%
GTGTGGATACAGGTTTAGAT+TGG - Chr2:26799847-26799866 None:intergenic 40.0%
GTTTCCTCAACAGGATTGAA+TGG - Chr2:26799521-26799540 None:intergenic 40.0%
! CACTCCCTGGTATTTTTCTT+TGG + Chr2:26800026-26800045 MsG0280008180.01.T01:CDS 40.0%
!! AAGGTGGTAATAAGCATTGG+AGG + Chr2:26799476-26799495 MsG0280008180.01.T01:CDS 40.0%
!! CAGGAGTTTTCAAGGAAACT+TGG + Chr2:26799393-26799412 MsG0280008180.01.T01:CDS 40.0%
!! GTAGTGGATTTCATTGGTCT+CGG - Chr2:26799765-26799784 None:intergenic 40.0%
AAACCTAGTGTAGTCCTTCC+AGG + Chr2:26799911-26799930 MsG0280008180.01.T01:CDS 45.0%
AAGTCGTCAAGTGTGGATAC+AGG - Chr2:26799857-26799876 None:intergenic 45.0%
AGGCTCAATGTGAAGCTTGT+CGG - Chr2:26799945-26799964 None:intergenic 45.0%
GGAAACTATCTGGATTACGC+GGG + Chr2:26799535-26799554 MsG0280008180.01.T01:CDS 45.0%
TCCACTACCGCAACTTGTAT+TGG + Chr2:26799777-26799796 MsG0280008180.01.T01:CDS 45.0%
TGGCAAAGCCCAAAATGAAG+TGG - Chr2:26799343-26799362 None:intergenic 45.0%
TTCCATACTACAGGTGACAC+AGG + Chr2:26799647-26799666 MsG0280008180.01.T01:CDS 45.0%
! TATAACTTGCCCTATGCACC+TGG - Chr2:26799687-26799706 None:intergenic 45.0%
! TGGATTTCATTGGTCTCGGA+TGG - Chr2:26799761-26799780 None:intergenic 45.0%
! TTCGTTTTGCCAGGTGCATA+GGG + Chr2:26799675-26799694 MsG0280008180.01.T01:CDS 45.0%
!! GTTGCGGTAGTGGATTTCAT+TGG - Chr2:26799771-26799790 None:intergenic 45.0%
!! TAGAGGCACAGGAGTTTTCA+AGG + Chr2:26799385-26799404 MsG0280008180.01.T01:CDS 45.0%
ACTCCTGGAAGGACTACACT+AGG - Chr2:26799917-26799936 None:intergenic 50.0%
AGCCTGTAGACAAGATGCCA+AGG + Chr2:26799960-26799979 MsG0280008180.01.T01:CDS 50.0%
CTATGCACCTGGCAAAACGA+AGG - Chr2:26799676-26799695 None:intergenic 50.0%
GACCTGTGTCACCTGTAGTA+TGG - Chr2:26799652-26799671 None:intergenic 50.0%
GCCAATACAAGTTGCGGTAG+TGG - Chr2:26799781-26799800 None:intergenic 50.0%
GCCTGTAGACAAGATGCCAA+GGG + Chr2:26799961-26799980 MsG0280008180.01.T01:CDS 50.0%
TACCAGGGAGTGATGCTATC+TGG - Chr2:26800018-26800037 None:intergenic 50.0%
TCCCTTGGCATCTTGTCTAC+AGG - Chr2:26799965-26799984 None:intergenic 50.0%
TTGAGCGTCATACTCCTCAG+TGG - Chr2:26799363-26799382 None:intergenic 50.0%
! ATTGGTCTCGGATGGAGCAA+TGG - Chr2:26799753-26799772 None:intergenic 50.0%
! CATTTTGGGCTTTGCCACTG+AGG + Chr2:26799346-26799365 MsG0280008180.01.T01:CDS 50.0%
! CTTCGTTTTGCCAGGTGCAT+AGG + Chr2:26799674-26799693 MsG0280008180.01.T01:CDS 50.0%
! GACGCTCAAAATAGAGGCAC+AGG + Chr2:26799374-26799393 MsG0280008180.01.T01:CDS 50.0%
CAGGGAGTGATGCTATCTGG+AGG - Chr2:26800015-26800034 None:intergenic 55.0%
CTCCAGATAGCATCACTCCC+TGG + Chr2:26800013-26800032 MsG0280008180.01.T01:CDS 55.0%
! CAGGTCACCTTCGTTTTGCC+AGG + Chr2:26799666-26799685 MsG0280008180.01.T01:CDS 55.0%
! CCAGCAATGAAGTGCTCCCT+TGG - Chr2:26799980-26799999 None:intergenic 55.0%
! TATCTGGATTACGCGGGCTG+TGG + Chr2:26799541-26799560 MsG0280008180.01.T01:CDS 55.0%
!! AGCTTGTCGGTGCTAACTCC+TGG - Chr2:26799932-26799951 None:intergenic 55.0%
!! CCAAGGGAGCACTTCATTGC+TGG + Chr2:26799977-26799996 MsG0280008180.01.T01:CDS 55.0%
!! TGTCGGTGCTAACTCCTGGA+AGG - Chr2:26799928-26799947 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 26799242 26800135 26799242 ID=MsG0280008180.01;Name=MsG0280008180.01
Chr2 mRNA 26799242 26800135 26799242 ID=MsG0280008180.01.T01;Parent=MsG0280008180.01;Name=MsG0280008180.01.T01;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|297
Chr2 exon 26799242 26800135 26799242 ID=MsG0280008180.01.T01:exon:18741;Parent=MsG0280008180.01.T01
Chr2 CDS 26799242 26800135 26799242 ID=MsG0280008180.01.T01:cds;Parent=MsG0280008180.01.T01
Gene Sequence

>MsG0280008180.01.T01

ATGTCTAAAATCTTTCGGGAATACATTGGTGTGAAGCCATTCTCAACAAATTTAAGGGATTTTCCAGTTCAAATAATCAAAACCAATATCTCAGAATTCCACTTCATTTTGGGCTTTGCCACTGAGGAGTATGACGCTCAAAATAGAGGCACAGGAGTTTTCAAGGAAACTTGGAATACTAGAGCATTCGGTCCAGAAGCAGTGAGAAATTTGAAGGGAAATAATCCAAATGTGAAGGTGGTAATAAGCATTGGAGGTCATGACGCCGTTAAAACTCCATTCAATCCTGTTGAGGAAACTATCTGGATTACGCGGGCTGTGGCTTCACTCAAAGTGATCATCCAAAAATACAAGGATCAAACCGGCAACATAATTGATGGCATTGACATTAATTATCTTAACGTCTTCCATACTACAGGTGACACAGGTCACCTTCGTTTTGCCAGGTGCATAGGGCAAGTTATAACTCAACTTAAGAATGATAATTATCTACGCATCAAGATCGTGTCCATTGCTCCATCCGAGACCAATGAAATCCACTACCGCAACTTGTATTGGCAAAATGAGGCCAATATAAATTGGGTTAACTACCAGTTCTACAACCAATCTAAACCTGTATCCACACTTGACGACTTTCTAAAACACTATGACCAGGTATCAAGAAACTACAAACCTAGTGTAGTCCTTCCAGGAGTTAGCACCGACAAGCTTCACATTGAGCCTGTAGACAAGATGCCAAGGGAGCACTTCATTGCTGGCTGCAGACATCTCCTCCAGATAGCATCACTCCCTGGTATTTTTCTTTGGAACGCTGATGACTCTACAATTCCTCTACCCAATGAGAATAAACCTTTCGTTTTAGAGGATATCTTGCAAAGCCTCCTCATAAGTTAA

Protein sequence

>MsG0280008180.01.T01

MSKIFREYIGVKPFSTNLRDFPVQIIKTNISEFHFILGFATEEYDAQNRGTGVFKETWNTRAFGPEAVRNLKGNNPNVKVVISIGGHDAVKTPFNPVEETIWITRAVASLKVIIQKYKDQTGNIIDGIDINYLNVFHTTGDTGHLRFARCIGQVITQLKNDNYLRIKIVSIAPSETNEIHYRNLYWQNEANINWVNYQFYNQSKPVSTLDDFLKHYDQVSRNYKPSVVLPGVSTDKLHIEPVDKMPREHFIAGCRHLLQIASLPGIFLWNADDSTIPLPNENKPFVLEDILQSLLIS*