AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007910.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007910.01.T01 MTR_2g035130 96.815 157 5 0 1 157 1 157 5.54e-107 302
MsG0280007910.01.T01 MTR_2g035150 86.076 158 22 0 1 158 1 158 3.67e-97 277
MsG0280007910.01.T01 MTR_2g035100 87.898 157 19 0 1 157 1 157 8.82e-96 273
MsG0280007910.01.T01 MTR_2g035120 86.076 158 22 0 1 158 1 158 4.24e-95 272
MsG0280007910.01.T01 MTR_2g035105 86.624 157 21 0 1 157 1 157 2.19e-94 270
MsG0280007910.01.T01 MTR_6g033450 68.987 158 49 0 1 158 1 158 1.36e-77 228
MsG0280007910.01.T01 MTR_4g120970 68.987 158 49 0 1 158 1 158 1.36e-77 228
MsG0280007910.01.T01 MTR_2g035320 61.146 157 61 0 1 157 1 157 1.56e-64 194
MsG0280007910.01.T01 MTR_2g035190 59.236 157 64 0 1 157 1 157 2.29e-62 189
MsG0280007910.01.T01 MTR_2g035210 57.962 157 66 0 1 157 1 157 2.29e-60 184
MsG0280007910.01.T01 MTR_2g035220 57.962 157 66 0 1 157 1 157 3.77e-59 181
MsG0280007910.01.T01 MTR_2g035170 56.688 157 68 0 1 157 1 157 9.23e-59 180
MsG0280007910.01.T01 MTR_2g435310 38.462 156 95 1 1 155 1 156 7.14e-38 127
MsG0280007910.01.T01 MTR_4g120760 39.490 157 94 1 1 156 1 157 1.46e-37 126
MsG0280007910.01.T01 MTR_4g120760 39.490 157 94 1 1 156 1 157 1.56e-37 126
MsG0280007910.01.T01 MTR_2g035320 57.576 99 42 0 59 157 24 122 6.01e-35 118
MsG0280007910.01.T01 MTR_4g120950 45.062 162 82 5 1 158 1 159 9.79e-34 117
MsG0280007910.01.T01 MTR_4g120940 61.798 89 31 1 70 158 35 120 1.52e-31 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAGCATAGTTGGTGGTTT+TGG 0.149266 2:+22802085 MsG0280007910.01.T01:CDS
CTTAGTAGATGATGTTAATT+TGG 0.195092 2:+22802054 MsG0280007910.01.T01:CDS
TGATGGCATCAATAACCTTT+GGG 0.301288 2:-22801229 None:intergenic
AGTAGCAAGTTAGCATATAA+TGG 0.315523 2:+22801118 MsG0280007910.01.T01:exon
AACGATATCAATACTCTTGA+TGG 0.325047 2:-22801246 None:intergenic
TTTGCAATGGTTCCTCCATT+TGG 0.327211 2:-22802160 None:intergenic
TATATGCTTTAGTTGTAATC+AGG 0.341112 2:-22802301 None:intergenic
GAAATCTTCTCCACTGTGTC+TGG 0.343745 2:-22802112 None:intergenic
TTGATGGCATCAATAACCTT+TGG 0.345179 2:-22801230 None:intergenic
GGCTCTTGAAGGTTATGTTT+TGG 0.350667 2:+22802273 MsG0280007910.01.T01:CDS
CACTTTGTGCAACACATACT+TGG 0.384404 2:-22802029 None:intergenic
TATATCATGTGTGCTTGATT+TGG 0.389507 2:+22802373 MsG0280007910.01.T01:three_prime_UTR
CATCAATAACCTTTGGGGTA+AGG 0.433870 2:-22801223 None:intergenic
ACTAGGTGCAAAATCACCTT+TGG 0.438881 2:-22802203 None:intergenic
TGCTTTGCTTGCTTTATACA+TGG 0.441815 2:+22802330 MsG0280007910.01.T01:three_prime_UTR
GTAGCAAGTTAGCATATAAT+GGG 0.454827 2:+22801119 MsG0280007910.01.T01:exon
ATCATGTGTGCTTGATTTGG+CGG 0.459609 2:+22802376 MsG0280007910.01.T01:three_prime_UTR
GCTTACCACTACAGCATAGT+TGG 0.461060 2:+22802076 MsG0280007910.01.T01:CDS
AAGAGTATTGATATCGTTGA+AGG 0.465901 2:+22801251 MsG0280007910.01.T01:CDS
AGTGGAGAAGATTTCATTTG+AGG 0.476098 2:+22802120 MsG0280007910.01.T01:CDS
AAATTGTCTGCAGGACCAAA+TGG 0.477800 2:+22802145 MsG0280007910.01.T01:CDS
AGAGCTTTGTAAAGTGTAGC+AGG 0.480318 2:-22801182 None:intergenic
ACTCACACTTCCACAACAAA+AGG 0.482152 2:-22802444 None:intergenic
TTTGAGGCTAAATTGTCTGC+AGG 0.494016 2:+22802136 MsG0280007910.01.T01:CDS
AATGGCAAAGCTAGGGGTGA+TGG 0.523022 2:+22802241 MsG0280007910.01.T01:CDS
TTCTTGAGTTCCTCTTCACT+AGG 0.524188 2:-22802220 None:intergenic
CAAGTATGTGTTGCACAAAG+TGG 0.538293 2:+22802030 MsG0280007910.01.T01:CDS
AACTCAAGAATGGCAAAGCT+AGG 0.540466 2:+22802233 MsG0280007910.01.T01:CDS
ACTCAAGAATGGCAAAGCTA+GGG 0.556431 2:+22802234 MsG0280007910.01.T01:CDS
GTTGAAGGAGACGGTGGCGC+CGG 0.560015 2:+22801266 MsG0280007910.01.T01:CDS
TGTAGCAGGAGCTACAATAG+AGG 0.565152 2:-22801168 None:intergenic
TATTATAATTTACTGAAGCA+AGG 0.566711 2:-22802489 None:intergenic
TACCACTACAGCATAGTTGG+TGG 0.567411 2:+22802079 MsG0280007910.01.T01:CDS
TTTCACACTAAGCTTTGCAA+TGG 0.568267 2:-22802173 None:intergenic
GATGGCATCAATAACCTTTG+GGG 0.578696 2:-22801228 None:intergenic
AAGTTGCTTAATTATTTGTG+TGG 0.582347 2:-22801063 None:intergenic
AGTGAAGAGGAACTCAAGAA+TGG 0.584353 2:+22802223 MsG0280007910.01.T01:CDS
ATTGATATCGTTGAAGGAGA+CGG 0.588878 2:+22801257 MsG0280007910.01.T01:CDS
ATATGCTAACTTGCTACTGA+AGG 0.594895 2:-22801113 None:intergenic
AACCACCAACTATGCTGTAG+TGG 0.607193 2:-22802081 None:intergenic
TGCTGACAACCTTACCCCAA+AGG 0.621335 2:+22801214 MsG0280007910.01.T01:CDS
CTCAAGAATGGCAAAGCTAG+GGG 0.641253 2:+22802235 MsG0280007910.01.T01:CDS
AGTGTGAAATACTTCACCAA+AGG 0.651010 2:+22802187 MsG0280007910.01.T01:CDS
AGCAGGAGCTACAATAGAGG+TGG 0.665165 2:-22801165 None:intergenic
TTGTCTGCAGGACCAAATGG+AGG 0.666387 2:+22802148 MsG0280007910.01.T01:CDS
GATATCGTTGAAGGAGACGG+TGG 0.715509 2:+22801260 MsG0280007910.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATATATAAAATAAT+AGG + Chr2:22801971-22801990 MsG0280007910.01.T01:intron 0.0%
!! ATCATTACTAGTATTATATA+AGG + Chr2:22801931-22801950 MsG0280007910.01.T01:intron 15.0%
!! GTGATAAAAAAATTGATAAA+CGG - Chr2:22801547-22801566 None:intergenic 15.0%
!! ACTCTTATGATTAACATATT+TGG + Chr2:22801456-22801475 MsG0280007910.01.T01:intron 20.0%
!! ATATTTGGTTGCTAATTAAT+TGG + Chr2:22801471-22801490 MsG0280007910.01.T01:intron 20.0%
!! TATTATAATTTACTGAAGCA+AGG - Chr2:22802492-22802511 None:intergenic 20.0%
! AAAATTGATAAACGGTAAAG+TGG - Chr2:22801539-22801558 None:intergenic 25.0%
! AGAAAAAGAGAGAATTACAT+TGG - Chr2:22802402-22802421 None:intergenic 25.0%
! CTTAGTAGATGATGTTAATT+TGG + Chr2:22802054-22802073 MsG0280007910.01.T01:CDS 25.0%
! CTTTCAAATATACCGTTAAA+AGG - Chr2:22801730-22801749 None:intergenic 25.0%
! GACTAAACTATACTTATTTC+CGG - Chr2:22801806-22801825 None:intergenic 25.0%
! TATATGCTTTAGTTGTAATC+AGG - Chr2:22802304-22802323 None:intergenic 25.0%
! TTAGTCAAAGACAAATTGTT+CGG - Chr2:22801848-22801867 None:intergenic 25.0%
!! AGAATGCTAGAATATTCTTT+TGG + Chr2:22801748-22801767 MsG0280007910.01.T01:intron 25.0%
AACACAATTGCTTGATTAAC+TGG + Chr2:22801885-22801904 MsG0280007910.01.T01:intron 30.0%
AACGATATCAATACTCTTGA+TGG - Chr2:22801249-22801268 None:intergenic 30.0%
AGTAGCAAGTTAGCATATAA+TGG + Chr2:22801118-22801137 MsG0280007910.01.T01:exon 30.0%
CTTAGTGATCTCATTCTTTA+TGG + Chr2:22801694-22801713 MsG0280007910.01.T01:intron 30.0%
GATCTCATTCTTTATGGAAT+AGG + Chr2:22801700-22801719 MsG0280007910.01.T01:intron 30.0%
GTAGCAAGTTAGCATATAAT+GGG + Chr2:22801119-22801138 MsG0280007910.01.T01:exon 30.0%
TAAAGAATGAGATCACTAAG+AGG - Chr2:22801694-22801713 None:intergenic 30.0%
TAGTCAAAGACAAATTGTTC+GGG - Chr2:22801847-22801866 None:intergenic 30.0%
TATATCATGTGTGCTTGATT+TGG + Chr2:22802373-22802392 MsG0280007910.01.T01:three_prime_UTR 30.0%
TCAAGTATACCAAATCAATG+TGG - Chr2:22801612-22801631 None:intergenic 30.0%
TTCTAATCTGAGTATCATTC+CGG + Chr2:22801784-22801803 MsG0280007910.01.T01:intron 30.0%
! ATCAAAGTGTCACAATTACT+AGG - Chr2:22801573-22801592 None:intergenic 30.0%
! GAAGAATAACCACATTGATT+TGG + Chr2:22801600-22801619 MsG0280007910.01.T01:intron 30.0%
! GACAAAAGTGATTTTCTTGA+TGG - Chr2:22801294-22801313 None:intergenic 30.0%
!! AAGAAAATCACTTTTGTCGA+AGG + Chr2:22801296-22801315 MsG0280007910.01.T01:CDS 30.0%
!! AAGAGTATTGATATCGTTGA+AGG + Chr2:22801251-22801270 MsG0280007910.01.T01:CDS 30.0%
!! CTATTTCTTTCCTTTTGTTG+TGG + Chr2:22802434-22802453 MsG0280007910.01.T01:three_prime_UTR 30.0%
AGTGGAGAAGATTTCATTTG+AGG + Chr2:22802120-22802139 MsG0280007910.01.T01:CDS 35.0%
AGTGTGAAATACTTCACCAA+AGG + Chr2:22802187-22802206 MsG0280007910.01.T01:CDS 35.0%
ATCTAACTGCGAAATTCACA+CGG + Chr2:22801362-22801381 MsG0280007910.01.T01:intron 35.0%
ATTGATATCGTTGAAGGAGA+CGG + Chr2:22801257-22801276 MsG0280007910.01.T01:CDS 35.0%
TGATGGCATCAATAACCTTT+GGG - Chr2:22801232-22801251 None:intergenic 35.0%
TGCTTTGCTTGCTTTATACA+TGG + Chr2:22802330-22802349 MsG0280007910.01.T01:three_prime_UTR 35.0%
TTTAACCAATGATGTGCAGA+TGG + Chr2:22802001-22802020 MsG0280007910.01.T01:intron 35.0%
TTTCACACTAAGCTTTGCAA+TGG - Chr2:22802176-22802195 None:intergenic 35.0%
! AATGGGTGTTTTCAACTTTG+AGG + Chr2:22801136-22801155 MsG0280007910.01.T01:exon 35.0%
! GGAATAGGTCATCCTTTTAA+CGG + Chr2:22801715-22801734 MsG0280007910.01.T01:intron 35.0%
! TCTGCACATCATTGGTTAAA+AGG - Chr2:22802001-22802020 None:intergenic 35.0%
! TTGATGGCATCAATAACCTT+TGG - Chr2:22801233-22801252 None:intergenic 35.0%
!! AGTGATTTTCTTGATGGTTC+CGG - Chr2:22801288-22801307 None:intergenic 35.0%
!! ATATGCTAACTTGCTACTGA+AGG - Chr2:22801116-22801135 None:intergenic 35.0%
AAATTGTCTGCAGGACCAAA+TGG + Chr2:22802145-22802164 MsG0280007910.01.T01:CDS 40.0%
AACTCAAGAATGGCAAAGCT+AGG + Chr2:22802233-22802252 MsG0280007910.01.T01:CDS 40.0%
ACTAGGTGCAAAATCACCTT+TGG - Chr2:22802206-22802225 None:intergenic 40.0%
ACTCAAGAATGGCAAAGCTA+GGG + Chr2:22802234-22802253 MsG0280007910.01.T01:CDS 40.0%
ACTCACACTTCCACAACAAA+AGG - Chr2:22802447-22802466 None:intergenic 40.0%
AGTGAAGAGGAACTCAAGAA+TGG + Chr2:22802223-22802242 MsG0280007910.01.T01:CDS 40.0%
CAAGTATGTGTTGCACAAAG+TGG + Chr2:22802030-22802049 MsG0280007910.01.T01:CDS 40.0%
CACTTTGTGCAACACATACT+TGG - Chr2:22802032-22802051 None:intergenic 40.0%
GATGGCATCAATAACCTTTG+GGG - Chr2:22801231-22801250 None:intergenic 40.0%
TTCTTGAGTTCCTCTTCACT+AGG - Chr2:22802223-22802242 None:intergenic 40.0%
TTTCACCATCTGCACATCAT+TGG - Chr2:22802009-22802028 None:intergenic 40.0%
TTTGCAATGGTTCCTCCATT+TGG - Chr2:22802163-22802182 None:intergenic 40.0%
! AGAGCTTTGTAAAGTGTAGC+AGG - Chr2:22801185-22801204 None:intergenic 40.0%
! GGCTCTTGAAGGTTATGTTT+TGG + Chr2:22802273-22802292 MsG0280007910.01.T01:CDS 40.0%
! TGATTTTGCACCTAGTGAAG+AGG + Chr2:22802210-22802229 MsG0280007910.01.T01:CDS 40.0%
! TTTGAGGCTAAATTGTCTGC+AGG + Chr2:22802136-22802155 MsG0280007910.01.T01:CDS 40.0%
!! ATCATGTGTGCTTGATTTGG+CGG + Chr2:22802376-22802395 MsG0280007910.01.T01:three_prime_UTR 40.0%
!! CATCAATAACCTTTGGGGTA+AGG - Chr2:22801226-22801245 None:intergenic 40.0%
!!! ACAGCATAGTTGGTGGTTTT+GGG + Chr2:22802086-22802105 MsG0280007910.01.T01:CDS 40.0%
!!! TACAGCATAGTTGGTGGTTT+TGG + Chr2:22802085-22802104 MsG0280007910.01.T01:CDS 40.0%
AACCACCAACTATGCTGTAG+TGG - Chr2:22802084-22802103 None:intergenic 45.0%
CTCAAGAATGGCAAAGCTAG+GGG + Chr2:22802235-22802254 MsG0280007910.01.T01:CDS 45.0%
GAAATCTTCTCCACTGTGTC+TGG - Chr2:22802115-22802134 None:intergenic 45.0%
GCTTACCACTACAGCATAGT+TGG + Chr2:22802076-22802095 MsG0280007910.01.T01:CDS 45.0%
TACCACTACAGCATAGTTGG+TGG + Chr2:22802079-22802098 MsG0280007910.01.T01:CDS 45.0%
TGTAGCAGGAGCTACAATAG+AGG - Chr2:22801171-22801190 None:intergenic 45.0%
!! GGTCTTTTCAAGGCTCTTGA+AGG + Chr2:22802262-22802281 MsG0280007910.01.T01:CDS 45.0%
!! TAGGGGTGATGGTCTTTTCA+AGG + Chr2:22802252-22802271 MsG0280007910.01.T01:CDS 45.0%
AATGGCAAAGCTAGGGGTGA+TGG + Chr2:22802241-22802260 MsG0280007910.01.T01:CDS 50.0%
AGCAGGAGCTACAATAGAGG+TGG - Chr2:22801168-22801187 None:intergenic 50.0%
GATATCGTTGAAGGAGACGG+TGG + Chr2:22801260-22801279 MsG0280007910.01.T01:CDS 50.0%
TGCTGACAACCTTACCCCAA+AGG + Chr2:22801214-22801233 MsG0280007910.01.T01:CDS 50.0%
TTGTCTGCAGGACCAAATGG+AGG + Chr2:22802148-22802167 MsG0280007910.01.T01:CDS 50.0%
! TTTTGGGCTTCCAGACACAG+TGG + Chr2:22802102-22802121 MsG0280007910.01.T01:CDS 50.0%
GTTGAAGGAGACGGTGGCGC+CGG + Chr2:22801266-22801285 MsG0280007910.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 22801065 22802526 22801065 ID=MsG0280007910.01;Name=MsG0280007910.01
Chr2 mRNA 22801065 22802526 22801065 ID=MsG0280007910.01.T01;Parent=MsG0280007910.01;Name=MsG0280007910.01.T01;_AED=0.50;_eAED=0.50;_QI=72|1|1|1|1|1|2|211|158
Chr2 exon 22801065 22801317 22801065 ID=MsG0280007910.01.T01:exon:13872;Parent=MsG0280007910.01.T01
Chr2 exon 22802020 22802526 22802020 ID=MsG0280007910.01.T01:exon:13873;Parent=MsG0280007910.01.T01
Chr2 five_prime_UTR 22801065 22801136 22801065 ID=MsG0280007910.01.T01:five_prime_utr;Parent=MsG0280007910.01.T01
Chr2 CDS 22801137 22801317 22801137 ID=MsG0280007910.01.T01:cds;Parent=MsG0280007910.01.T01
Chr2 CDS 22802020 22802315 22802020 ID=MsG0280007910.01.T01:cds;Parent=MsG0280007910.01.T01
Chr2 three_prime_UTR 22802316 22802526 22802316 ID=MsG0280007910.01.T01:three_prime_utr;Parent=MsG0280007910.01.T01
Gene Sequence

>MsG0280007910.01.T01

ATGGGTGTTTTCAACTTTGAGGATGAAACCACCTCTATTGTAGCTCCTGCTACACTTTACAAAGCTCTTGTTACAGATGCTGACAACCTTACCCCAAAGGTTATTGATGCCATCAAGAGTATTGATATCGTTGAAGGAGACGGTGGCGCCGGAACCATCAAGAAAATCACTTTTGTCGAAGATGGTGAAACCAAGTATGTGTTGCACAAAGTGGACTTAGTAGATGATGTTAATTTGGCTTACCACTACAGCATAGTTGGTGGTTTTGGGCTTCCAGACACAGTGGAGAAGATTTCATTTGAGGCTAAATTGTCTGCAGGACCAAATGGAGGAACCATTGCAAAGCTTAGTGTGAAATACTTCACCAAAGGTGATTTTGCACCTAGTGAAGAGGAACTCAAGAATGGCAAAGCTAGGGGTGATGGTCTTTTCAAGGCTCTTGAAGGTTATGTTTTGGCAAATCCTGATTACAACTAA

Protein sequence

>MsG0280007910.01.T01

MGVFNFEDETTSIVAPATLYKALVTDADNLTPKVIDAIKSIDIVEGDGGAGTIKKITFVEDGETKYVLHKVDLVDDVNLAYHYSIVGGFGLPDTVEKISFEAKLSAGPNGGTIAKLSVKYFTKGDFAPSEEELKNGKARGDGLFKALEGYVLANPDYN*