AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007513.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007513.01.T01 MTR_2g026760 94.488 127 7 0 1 127 1 127 1.36e-82 238
MsG0280007513.01.T01 MTR_4g108150 55.906 127 50 3 1 126 1 122 1.96e-36 121
MsG0280007513.01.T01 MTR_4g101260 55.224 134 51 3 1 127 1 132 7.14e-36 120
MsG0280007513.01.T01 MTR_4g101330 42.857 168 54 5 1 126 1 168 2.32e-31 109
MsG0280007513.01.T01 MTR_4g101280 47.761 134 55 3 1 119 1 134 8.91e-30 105
MsG0280007513.01.T01 MTR_1g012690 58.427 89 36 1 40 127 66 154 1.63e-28 102
MsG0280007513.01.T01 MTR_1g012710 60.241 83 32 1 46 127 69 151 4.13e-27 98.6
MsG0280007513.01.T01 MTR_1g012700 57.303 89 37 1 40 127 76 164 1.51e-26 97.4
MsG0280007513.01.T01 MTR_1g012630 60.241 83 32 1 46 127 128 210 2.19e-26 98.2
MsG0280007513.01.T01 MTR_3g106740 61.176 85 31 2 44 126 45 129 4.30e-26 95.1
MsG0280007513.01.T01 MTR_4g101310 48.031 127 59 4 7 127 9 134 1.33e-25 94.0
MsG0280007513.01.T01 MTR_3g106750 55.814 86 36 2 43 126 43 128 1.52e-18 75.9
MsG0280007513.01.T01 MTR_4g101270 52.381 84 37 3 45 126 104 186 2.82e-17 73.9
MsG0280007513.01.T01 MTR_5g012340 41.860 86 47 3 43 126 92 176 8.93e-17 72.8
MsG0280007513.01.T01 MTR_2g026920 50.633 79 36 2 49 126 118 194 1.58e-16 72.4
MsG0280007513.01.T01 MTR_5g012380 41.860 86 47 3 43 126 85 169 2.28e-16 71.2
MsG0280007513.01.T01 MTR_2g026930 50.633 79 36 2 49 126 122 198 2.30e-16 72.0
MsG0280007513.01.T01 MTR_2g026820 52.439 82 33 3 46 126 539 615 2.54e-16 74.3
MsG0280007513.01.T01 MTR_2g026775 44.872 78 37 3 53 127 686 760 9.08e-13 63.9
MsG0280007513.01.T01 MTR_3g082100 41.860 86 47 3 43 126 251 335 2.93e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007513.01.T01 AT5G46900 59.231 130 45 5 1 126 1 126 9.69e-35 117
MsG0280007513.01.T01 AT4G12550 49.219 128 45 3 1 126 1 110 7.00e-34 114
MsG0280007513.01.T01 AT5G46890 58.462 130 46 5 1 126 1 126 4.71e-33 112
MsG0280007513.01.T01 AT4G12545 50.781 128 40 4 1 126 1 107 8.64e-33 111
MsG0280007513.01.T01 AT4G12520 50.370 135 51 5 1 126 1 128 1.12e-32 112
MsG0280007513.01.T01 AT4G12510 50.370 135 51 5 1 126 1 128 1.12e-32 112
MsG0280007513.01.T01 AT2G45180 52.000 125 55 2 8 127 10 134 4.68e-32 110
MsG0280007513.01.T01 AT4G12490 43.169 183 45 7 1 126 1 181 4.82e-31 109
MsG0280007513.01.T01 AT4G12500 42.373 177 50 6 1 126 1 176 4.11e-30 107
MsG0280007513.01.T01 AT1G12100 48.760 121 57 5 3 119 4 123 4.95e-30 105
MsG0280007513.01.T01 AT4G12470 45.625 160 53 5 1 126 1 160 1.03e-29 105
MsG0280007513.01.T01 AT1G62510 67.470 83 25 2 46 126 67 149 1.11e-29 105
MsG0280007513.01.T01 AT4G12480 46.429 168 49 6 1 127 1 168 2.25e-28 102
MsG0280007513.01.T01 AT1G12090 65.000 80 26 2 49 126 58 137 2.93e-26 95.9
MsG0280007513.01.T01 AT4G22460 59.551 89 32 2 38 122 39 127 3.81e-26 95.5
MsG0280007513.01.T01 AT4G12530 40.323 124 57 2 4 126 8 115 8.54e-24 89.0
MsG0280007513.01.T01 AT4G00165 53.012 83 37 2 46 126 46 128 1.38e-23 89.0
MsG0280007513.01.T01 AT4G00165 53.012 83 37 2 46 126 46 128 1.38e-23 89.0
MsG0280007513.01.T01 AT2G10940 50.575 87 40 3 43 127 205 290 4.12e-18 78.2
MsG0280007513.01.T01 AT2G10940 50.575 87 40 3 43 127 205 290 4.12e-18 78.2
MsG0280007513.01.T01 AT1G62500 47.674 86 42 3 43 126 209 293 1.18e-15 71.6
MsG0280007513.01.T01 AT3G22142 46.067 89 35 5 45 126 1396 1478 5.28e-15 70.5
MsG0280007513.01.T01 AT4G15160 46.512 86 41 4 45 126 98 182 1.17e-13 64.7
MsG0280007513.01.T01 AT4G15160 46.512 86 41 4 45 126 180 264 3.22e-13 64.7
MsG0280007513.01.T01 AT3G22120 47.059 85 41 4 45 126 266 349 9.74e-13 63.9
MsG0280007513.01.T01 AT3G22120 47.059 85 41 4 45 126 249 332 1.40e-12 63.2

Find 39 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTGGAACTGGGATTTC+AGG 0.160812 2:-16453714 None:intergenic
GACCAAGGACATTAGCTTTA+AGG 0.172237 2:-16453917 None:intergenic
CCTTTGTGAAATGGCTTATA+AGG 0.189298 2:+16453626 None:intergenic
CCTAACTTAAGTGCATTTAT+AGG 0.239997 2:-16453774 None:intergenic
AACTTGGTCAAAGTTAAATT+AGG 0.277653 2:+16453816 MsG0280007513.01.T01:CDS
TTTGGAGAAGGGTCATATAT+TGG 0.304460 2:-16453741 None:intergenic
CCTTGTTGCTCACTCATTCA+AGG 0.350456 2:+16453855 MsG0280007513.01.T01:CDS
ATTGGGTCTGGGATTGGAAC+TGG 0.356899 2:-16453723 None:intergenic
TTGGAGAAGGGTCATATATT+GGG 0.368675 2:-16453740 None:intergenic
ACTTGGTCAAAGTTAAATTA+GGG 0.373507 2:+16453817 MsG0280007513.01.T01:CDS
AGAATGACACTCAAGGAAAT+AGG 0.388403 2:-16453954 None:intergenic
ACCAAGGACATTAGCTTTAA+GGG 0.388445 2:-16453916 None:intergenic
GAAGGGTCATATATTGGGTC+TGG 0.389255 2:-16453735 None:intergenic
TTTATAGGACATGTACCCTT+TGG 0.402117 2:-16453759 None:intergenic
CCTATAAATGCACTTAAGTT+AGG 0.406380 2:+16453774 MsG0280007513.01.T01:CDS
TCATATATTGGGTCTGGGAT+TGG 0.411255 2:-16453729 None:intergenic
GGATTGGAACTGGGATTTCA+GGG 0.445515 2:-16453713 None:intergenic
GCCCTTAAAGCTAATGTCCT+TGG 0.495398 2:+16453915 MsG0280007513.01.T01:CDS
TTGGGTCTGGGATTGGAACT+GGG 0.520356 2:-16453722 None:intergenic
GAGCAACAAGGGAGTGTTGG+TGG 0.532377 2:-16453843 None:intergenic
ATATATGACCCTTCTCCAAA+GGG 0.532446 2:+16453744 MsG0280007513.01.T01:CDS
GGCAGTGCAAAGGCAAGCAG+CGG 0.537538 2:-16453895 None:intergenic
GGACATGTACCCTTTGGAGA+AGG 0.541046 2:-16453753 None:intergenic
AATATATGACCCTTCTCCAA+AGG 0.548759 2:+16453743 MsG0280007513.01.T01:CDS
AAGGGTCATATATTGGGTCT+GGG 0.551516 2:-16453734 None:intergenic
AGCAACAAGGGAGTGTTGGT+GGG 0.555115 2:-16453842 None:intergenic
AGGAAATAGGAACATCAAGA+TGG 0.560269 2:-16453941 None:intergenic
GACATGTACCCTTTGGAGAA+GGG 0.569046 2:-16453752 None:intergenic
AATTGTGGAAGGAACAACTC+TGG 0.577791 2:+16453981 MsG0280007513.01.T01:CDS
TAGCTTTAAGGGCAGTGCAA+AGG 0.582397 2:-16453905 None:intergenic
AGTGTCATTCTCAACAATTG+TGG 0.590474 2:+16453966 MsG0280007513.01.T01:CDS
CTTGAATGAGTGAGCAACAA+GGG 0.595201 2:-16453854 None:intergenic
GCAACAAGGGAGTGTTGGTG+GGG 0.610643 2:-16453841 None:intergenic
CCTTGAATGAGTGAGCAACA+AGG 0.614845 2:-16453855 None:intergenic
AGTGAGCAACAAGGGAGTGT+TGG 0.617453 2:-16453846 None:intergenic
ATTGTTGAGAATGACACTCA+AGG 0.634515 2:-16453961 None:intergenic
TCATTCTCAACAATTGTGGA+AGG 0.636203 2:+16453970 MsG0280007513.01.T01:CDS
GTGTGCAAAAGTGTTGAACT+TGG 0.636381 2:+16453800 MsG0280007513.01.T01:CDS
GAACATCAAGATGGAGACCA+AGG 0.649528 2:-16453932 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATATTATTTTTTTCACTA+TGG + Chr2:16453674-16453693 MsG0280007513.01.T01:CDS 15.0%
! AACTTGGTCAAAGTTAAATT+AGG + Chr2:16453816-16453835 MsG0280007513.01.T01:CDS 25.0%
! ACTTGGTCAAAGTTAAATTA+GGG + Chr2:16453817-16453836 MsG0280007513.01.T01:CDS 25.0%
CCTAACTTAAGTGCATTTAT+AGG - Chr2:16453777-16453796 None:intergenic 30.0%
CCTATAAATGCACTTAAGTT+AGG + Chr2:16453774-16453793 MsG0280007513.01.T01:CDS 30.0%
AATATATGACCCTTCTCCAA+AGG + Chr2:16453743-16453762 MsG0280007513.01.T01:CDS 35.0%
AGAATGACACTCAAGGAAAT+AGG - Chr2:16453957-16453976 None:intergenic 35.0%
AGGAAATAGGAACATCAAGA+TGG - Chr2:16453944-16453963 None:intergenic 35.0%
ATATATGACCCTTCTCCAAA+GGG + Chr2:16453744-16453763 MsG0280007513.01.T01:CDS 35.0%
ATTGTTGAGAATGACACTCA+AGG - Chr2:16453964-16453983 None:intergenic 35.0%
TCATTCTCAACAATTGTGGA+AGG + Chr2:16453970-16453989 MsG0280007513.01.T01:CDS 35.0%
TTGGAGAAGGGTCATATATT+GGG - Chr2:16453743-16453762 None:intergenic 35.0%
TTTATAGGACATGTACCCTT+TGG - Chr2:16453762-16453781 None:intergenic 35.0%
TTTGGAGAAGGGTCATATAT+TGG - Chr2:16453744-16453763 None:intergenic 35.0%
! AGTGTCATTCTCAACAATTG+TGG + Chr2:16453966-16453985 MsG0280007513.01.T01:CDS 35.0%
!! ACCAAGGACATTAGCTTTAA+GGG - Chr2:16453919-16453938 None:intergenic 35.0%
AAGGGTCATATATTGGGTCT+GGG - Chr2:16453737-16453756 None:intergenic 40.0%
AATTGTGGAAGGAACAACTC+TGG + Chr2:16453981-16454000 MsG0280007513.01.T01:CDS 40.0%
CTTGAATGAGTGAGCAACAA+GGG - Chr2:16453857-16453876 None:intergenic 40.0%
! TCATATATTGGGTCTGGGAT+TGG - Chr2:16453732-16453751 None:intergenic 40.0%
!! GACCAAGGACATTAGCTTTA+AGG - Chr2:16453920-16453939 None:intergenic 40.0%
!! GTGTGCAAAAGTGTTGAACT+TGG + Chr2:16453800-16453819 MsG0280007513.01.T01:CDS 40.0%
CCTTGAATGAGTGAGCAACA+AGG - Chr2:16453858-16453877 None:intergenic 45.0%
CCTTGTTGCTCACTCATTCA+AGG + Chr2:16453855-16453874 MsG0280007513.01.T01:CDS 45.0%
GAACATCAAGATGGAGACCA+AGG - Chr2:16453935-16453954 None:intergenic 45.0%
GAAGGGTCATATATTGGGTC+TGG - Chr2:16453738-16453757 None:intergenic 45.0%
GCCCTTAAAGCTAATGTCCT+TGG + Chr2:16453915-16453934 MsG0280007513.01.T01:CDS 45.0%
GGATTGGAACTGGGATTTCA+GGG - Chr2:16453716-16453735 None:intergenic 45.0%
! GACATGTACCCTTTGGAGAA+GGG - Chr2:16453755-16453774 None:intergenic 45.0%
!! TAGCTTTAAGGGCAGTGCAA+AGG - Chr2:16453908-16453927 None:intergenic 45.0%
GGGATTGGAACTGGGATTTC+AGG - Chr2:16453717-16453736 None:intergenic 50.0%
! AGTGAGCAACAAGGGAGTGT+TGG - Chr2:16453849-16453868 None:intergenic 50.0%
! ATTGGGTCTGGGATTGGAAC+TGG - Chr2:16453726-16453745 None:intergenic 50.0%
! GGACATGTACCCTTTGGAGA+AGG - Chr2:16453756-16453775 None:intergenic 50.0%
! TTGGGTCTGGGATTGGAACT+GGG - Chr2:16453725-16453744 None:intergenic 50.0%
!! AGCAACAAGGGAGTGTTGGT+GGG - Chr2:16453845-16453864 None:intergenic 50.0%
!! GAGCAACAAGGGAGTGTTGG+TGG - Chr2:16453846-16453865 None:intergenic 55.0%
!! GCAACAAGGGAGTGTTGGTG+GGG - Chr2:16453844-16453863 None:intergenic 55.0%
GGCAGTGCAAAGGCAAGCAG+CGG - Chr2:16453898-16453917 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 16453636 16454019 16453636 ID=MsG0280007513.01;Name=MsG0280007513.01
Chr2 mRNA 16453636 16454019 16453636 ID=MsG0280007513.01.T01;Parent=MsG0280007513.01;Name=MsG0280007513.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|127
Chr2 exon 16453636 16454019 16453636 ID=MsG0280007513.01.T01:exon:3493;Parent=MsG0280007513.01.T01
Chr2 CDS 16453636 16454019 16453636 ID=MsG0280007513.01.T01:cds;Parent=MsG0280007513.01.T01
Gene Sequence

>MsG0280007513.01.T01

ATGGCTTATAAGGTTAATGTTGCATTTTTCTTAAGTCTCAATATTATTTTTTTCACTATGGTGAATTGCAACTATGTCCCTGAAATCCCAGTTCCAATCCCAGACCCAATATATGACCCTTCTCCAAAGGGTACATGTCCTATAAATGCACTTAAGTTAGGTGTGTGTGCAAAAGTGTTGAACTTGGTCAAAGTTAAATTAGGGTCCCCACCAACACTCCCTTGTTGCTCACTCATTCAAGGTCTTGCTGATCTTGAAGCCGCTGCTTGCCTTTGCACTGCCCTTAAAGCTAATGTCCTTGGTCTCCATCTTGATGTTCCTATTTCCTTGAGTGTCATTCTCAACAATTGTGGAAGGAACAACTCTGGTTTCAAGTGCACTTAG

Protein sequence

>MsG0280007513.01.T01

MAYKVNVAFFLSLNIIFFTMVNCNYVPEIPVPIPDPIYDPSPKGTCPINALKLGVCAKVLNLVKVKLGSPPTLPCCSLIQGLADLEAAACLCTALKANVLGLHLDVPISLSVILNNCGRNNSGFKCT*