AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046434.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046434.01.T01 MTR_4g101260 95.455 132 5 1 1 131 1 132 5.89e-86 246
MsG0880046434.01.T01 MTR_4g108150 92.366 131 2 1 1 131 1 123 1.02e-80 233
MsG0880046434.01.T01 MTR_2g026760 54.688 128 52 3 4 131 6 127 3.66e-38 125
MsG0880046434.01.T01 MTR_4g101280 54.015 137 55 2 3 131 7 143 9.41e-38 125
MsG0880046434.01.T01 MTR_4g101330 47.853 163 49 3 3 130 7 168 3.68e-37 124
MsG0880046434.01.T01 MTR_1g012690 61.053 95 37 0 37 131 60 154 6.22e-36 121
MsG0880046434.01.T01 MTR_1g012700 55.833 120 39 3 26 131 45 164 1.35e-35 120
MsG0880046434.01.T01 MTR_1g012710 63.855 83 30 0 49 131 69 151 3.62e-34 116
MsG0880046434.01.T01 MTR_1g012630 66.265 83 28 0 49 131 128 210 5.57e-34 117
MsG0880046434.01.T01 MTR_3g106740 68.675 83 25 1 49 130 47 129 9.30e-34 114
MsG0880046434.01.T01 MTR_4g101310 45.802 131 68 2 2 131 6 134 1.02e-29 104
MsG0880046434.01.T01 MTR_3g106750 60.714 84 32 1 49 131 46 129 3.79e-24 90.5
MsG0880046434.01.T01 MTR_4g101270 53.571 84 37 2 48 130 104 186 3.57e-21 84.3
MsG0880046434.01.T01 MTR_5g012340 40.000 105 45 3 43 130 73 176 8.19e-20 80.5
MsG0880046434.01.T01 MTR_2g026930 46.835 79 40 1 52 130 122 198 2.84e-17 74.3
MsG0880046434.01.T01 MTR_2g026920 46.835 79 40 1 52 130 118 194 3.03e-17 74.3
MsG0880046434.01.T01 MTR_2g026775 46.053 76 36 2 58 131 688 760 5.48e-16 73.6
MsG0880046434.01.T01 MTR_2g026820 44.872 78 38 3 54 130 542 615 1.02e-12 63.9
MsG0880046434.01.T01 MTR_3g082100 42.857 84 46 2 48 130 253 335 1.25e-12 63.5
MsG0880046434.01.T01 MTR_4g115360 48.235 85 42 2 48 131 150 233 6.15e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046434.01.T01 AT2G45180 62.222 135 46 2 1 131 1 134 4.52e-47 148
MsG0880046434.01.T01 AT5G46890 64.341 129 41 4 3 131 4 127 3.95e-42 135
MsG0880046434.01.T01 AT4G12520 52.941 136 52 3 1 131 1 129 1.88e-38 126
MsG0880046434.01.T01 AT4G12510 52.941 136 52 3 1 131 1 129 1.88e-38 126
MsG0880046434.01.T01 AT5G46900 62.791 129 43 3 3 131 4 127 4.42e-38 125
MsG0880046434.01.T01 AT1G62510 73.494 83 21 1 49 130 67 149 1.38e-36 122
MsG0880046434.01.T01 AT4G00165 52.846 123 43 3 12 130 17 128 3.71e-36 120
MsG0880046434.01.T01 AT4G00165 52.846 123 43 3 12 130 17 128 3.71e-36 120
MsG0880046434.01.T01 AT4G12480 40.476 168 63 3 1 131 1 168 6.60e-34 116
MsG0880046434.01.T01 AT4G12490 41.530 183 54 4 1 131 1 182 9.08e-34 116
MsG0880046434.01.T01 AT4G12550 49.219 128 41 2 5 131 7 111 1.04e-33 114
MsG0880046434.01.T01 AT1G12090 72.840 81 21 1 51 130 57 137 1.39e-33 114
MsG0880046434.01.T01 AT4G22460 57.692 104 41 3 26 128 29 130 2.38e-33 114
MsG0880046434.01.T01 AT4G12545 48.462 130 40 2 3 131 5 108 2.17e-32 110
MsG0880046434.01.T01 AT4G12470 42.236 161 63 3 1 131 1 161 8.15e-32 110
MsG0880046434.01.T01 AT4G12500 61.905 84 31 1 49 131 94 177 1.30e-31 110
MsG0880046434.01.T01 AT1G12100 46.094 128 62 3 6 131 10 132 9.22e-30 104
MsG0880046434.01.T01 AT4G12530 55.422 83 37 0 48 130 33 115 3.00e-27 97.8
MsG0880046434.01.T01 AT3G22142 52.381 84 38 2 48 130 1396 1478 1.76e-19 83.6
MsG0880046434.01.T01 AT2G10940 49.412 85 41 2 48 131 207 290 1.34e-18 79.7
MsG0880046434.01.T01 AT2G10940 49.412 85 41 2 48 131 207 290 1.34e-18 79.7
MsG0880046434.01.T01 AT3G22120 54.118 85 36 3 48 130 266 349 4.16e-18 79.0
MsG0880046434.01.T01 AT1G62500 47.126 87 44 2 45 130 208 293 4.67e-18 78.2
MsG0880046434.01.T01 AT3G22120 54.118 85 36 3 48 130 249 332 8.26e-18 77.8
MsG0880046434.01.T01 AT4G15160 51.163 86 38 4 48 130 98 182 3.40e-17 74.3
MsG0880046434.01.T01 AT4G15160 51.163 86 38 4 48 130 180 264 1.15e-16 74.3
MsG0880046434.01.T01 AT4G22490 32.584 89 58 1 42 130 30 116 3.04e-13 62.0
MsG0880046434.01.T01 AT4G22520 29.231 130 73 3 1 130 7 117 5.26e-12 58.9

Find 38 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATTAATGGGAAGATTA+AGG 0.239655 8:+73339348 None:intergenic
AAGAACCAAGCTCAAATTAA+TGG 0.256221 8:+73339337 None:intergenic
TGGTGTGTGTGCTGATGTAT+TGG 0.312025 8:-73339498 MsG0880046434.01.T01:CDS
GCTCTTAAGGCTAATATCTT+AGG 0.323048 8:-73339377 MsG0880046434.01.T01:CDS
GTGCCTTTGTACTGCTCTTA+AGG 0.328363 8:-73339390 MsG0880046434.01.T01:CDS
CCTAGAGACACAATTAAGTT+TGG 0.348121 8:-73339518 MsG0880046434.01.T01:CDS
GGTGTGTGTGCTGATGTATT+GGG 0.364523 8:-73339497 MsG0880046434.01.T01:CDS
CCATGCTGCTCTCTCATTGA+TGG 0.378656 8:-73339437 MsG0880046434.01.T01:CDS
AGGACAAGTTGGATATTTAG+AGG 0.384493 8:+73339538 None:intergenic
AAGGTGGATGTGGGTGTTTG+TGG 0.411134 8:+73339561 None:intergenic
AGAGAGCAGCATGGTGTCTT+TGG 0.413985 8:+73339446 None:intergenic
AGAACCAAGCTCAAATTAAT+GGG 0.417409 8:+73339338 None:intergenic
TAAGCACACACGAAACCCTT+TGG 0.418402 8:+73339293 None:intergenic
TGTGATCTTTGTGAGGTGGT+GGG 0.421624 8:+73339585 None:intergenic
GTTCTTAACTATTGTGGAAA+GGG 0.421891 8:-73339320 MsG0880046434.01.T01:CDS
CTGTGATCTTTGTGAGGTGG+TGG 0.426802 8:+73339584 None:intergenic
CAAGTTGGATATTTAGAGGA+AGG 0.430718 8:+73339542 None:intergenic
AGCTTGGTTCTTAACTATTG+TGG 0.458633 8:-73339326 MsG0880046434.01.T01:CDS
GTGATCTTTGTGAGGTGGTG+GGG 0.458860 8:+73339586 None:intergenic
TGATCTTTGTGAGGTGGTGG+GGG 0.471644 8:+73339587 None:intergenic
GAGCAGCATGGTGTCTTTGG+TGG 0.477521 8:+73339449 None:intergenic
TATTTAGAGGAAGGTGGATG+TGG 0.477977 8:+73339551 None:intergenic
GCAAAGGAGCATAGCAACCT+TGG 0.496091 8:+73339658 None:intergenic
ATCATTCCAAATGGCTTCCA+AGG 0.499837 8:-73339675 None:intergenic
TCTTCCCATTAATTTGAGCT+TGG 0.503356 8:-73339342 MsG0880046434.01.T01:CDS
ATTGTGTCTCTAGGACAAGT+TGG 0.512609 8:+73339527 None:intergenic
GGTTTGATTAATGTTGAGCT+TGG 0.521860 8:-73339476 MsG0880046434.01.T01:CDS
GGTTCTTAACTATTGTGGAA+AGG 0.535979 8:-73339321 MsG0880046434.01.T01:CDS
CCAAACTTAATTGTGTCTCT+AGG 0.547203 8:+73339518 None:intergenic
GTTGGATATTTAGAGGAAGG+TGG 0.548060 8:+73339545 None:intergenic
TTGTGGAAAGGGAGTTCCAA+AGG 0.553018 8:-73339309 MsG0880046434.01.T01:CDS
ATTTAGAGGAAGGTGGATGT+GGG 0.563461 8:+73339552 None:intergenic
TGTGGAAAGGGAGTTCCAAA+GGG 0.568343 8:-73339308 MsG0880046434.01.T01:CDS
TGGCTGTGATCTTTGTGAGG+TGG 0.576896 8:+73339581 None:intergenic
AAAATAGGATGTTGAGGCAA+AGG 0.587552 8:+73339642 None:intergenic
TTGTGGCTGTGATCTTTGTG+AGG 0.617514 8:+73339578 None:intergenic
TAGCCTTAAGAGCAGTACAA+AGG 0.662253 8:+73339387 None:intergenic
CCATCAATGAGAGAGCAGCA+TGG 0.704306 8:+73339437 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAAATTAATGGGAAGATTA+AGG + Chr8:73339612-73339631 None:intergenic 25.0%
AAGAACCAAGCTCAAATTAA+TGG + Chr8:73339623-73339642 None:intergenic 30.0%
AGAACCAAGCTCAAATTAAT+GGG + Chr8:73339622-73339641 None:intergenic 30.0%
GTTCTTAACTATTGTGGAAA+GGG - Chr8:73339637-73339656 MsG0880046434.01.T01:CDS 30.0%
AAAATAGGATGTTGAGGCAA+AGG + Chr8:73339318-73339337 None:intergenic 35.0%
AGCTTACCACAGTGAAAAAT+AGG + Chr8:73339333-73339352 None:intergenic 35.0%
CAGTGAAAAATAGGATGTTG+AGG + Chr8:73339324-73339343 None:intergenic 35.0%
CCAAACTTAATTGTGTCTCT+AGG + Chr8:73339442-73339461 None:intergenic 35.0%
CCTAGAGACACAATTAAGTT+TGG - Chr8:73339439-73339458 MsG0880046434.01.T01:CDS 35.0%
GCTCTTAAGGCTAATATCTT+AGG - Chr8:73339580-73339599 MsG0880046434.01.T01:CDS 35.0%
GGTTCTTAACTATTGTGGAA+AGG - Chr8:73339636-73339655 MsG0880046434.01.T01:CDS 35.0%
GGTTTGATTAATGTTGAGCT+TGG - Chr8:73339481-73339500 MsG0880046434.01.T01:CDS 35.0%
! AGCTTGGTTCTTAACTATTG+TGG - Chr8:73339631-73339650 MsG0880046434.01.T01:CDS 35.0%
! AGGACAAGTTGGATATTTAG+AGG + Chr8:73339422-73339441 None:intergenic 35.0%
! CAAGTTGGATATTTAGAGGA+AGG + Chr8:73339418-73339437 None:intergenic 35.0%
! TCTTCCCATTAATTTGAGCT+TGG - Chr8:73339615-73339634 MsG0880046434.01.T01:CDS 35.0%
!! CAACATCCTATTTTTCACTG+TGG - Chr8:73339324-73339343 MsG0880046434.01.T01:CDS 35.0%
ATTTAGAGGAAGGTGGATGT+GGG + Chr8:73339408-73339427 None:intergenic 40.0%
GTTGGATATTTAGAGGAAGG+TGG + Chr8:73339415-73339434 None:intergenic 40.0%
TATTTAGAGGAAGGTGGATG+TGG + Chr8:73339409-73339428 None:intergenic 40.0%
! TAGCCTTAAGAGCAGTACAA+AGG + Chr8:73339573-73339592 None:intergenic 40.0%
!! ATTGTGTCTCTAGGACAAGT+TGG + Chr8:73339433-73339452 None:intergenic 40.0%
GTGCCTTTGTACTGCTCTTA+AGG - Chr8:73339567-73339586 MsG0880046434.01.T01:CDS 45.0%
TAAGCACACACGAAACCCTT+TGG + Chr8:73339667-73339686 None:intergenic 45.0%
TGTGATCTTTGTGAGGTGGT+GGG + Chr8:73339375-73339394 None:intergenic 45.0%
TGTGGAAAGGGAGTTCCAAA+GGG - Chr8:73339649-73339668 MsG0880046434.01.T01:CDS 45.0%
TTGTGGAAAGGGAGTTCCAA+AGG - Chr8:73339648-73339667 MsG0880046434.01.T01:CDS 45.0%
TTGTGGCTGTGATCTTTGTG+AGG + Chr8:73339382-73339401 None:intergenic 45.0%
! GGTGTGTGTGCTGATGTATT+GGG - Chr8:73339460-73339479 MsG0880046434.01.T01:CDS 45.0%
! TGGTGTGTGTGCTGATGTAT+TGG - Chr8:73339459-73339478 MsG0880046434.01.T01:CDS 45.0%
AAGGTGGATGTGGGTGTTTG+TGG + Chr8:73339399-73339418 None:intergenic 50.0%
AGAGAGCAGCATGGTGTCTT+TGG + Chr8:73339514-73339533 None:intergenic 50.0%
CCATCAATGAGAGAGCAGCA+TGG + Chr8:73339523-73339542 None:intergenic 50.0%
CCATGCTGCTCTCTCATTGA+TGG - Chr8:73339520-73339539 MsG0880046434.01.T01:CDS 50.0%
CTGTGATCTTTGTGAGGTGG+TGG + Chr8:73339376-73339395 None:intergenic 50.0%
GTGATCTTTGTGAGGTGGTG+GGG + Chr8:73339374-73339393 None:intergenic 50.0%
TGGCTGTGATCTTTGTGAGG+TGG + Chr8:73339379-73339398 None:intergenic 50.0%
! GCAAAGGAGCATAGCAACCT+TGG + Chr8:73339302-73339321 None:intergenic 50.0%
! TGATCTTTGTGAGGTGGTGG+GGG + Chr8:73339373-73339392 None:intergenic 50.0%
GAGCAGCATGGTGTCTTTGG+TGG + Chr8:73339511-73339530 None:intergenic 55.0%
!! TTGTGAGGTGGTGGGGGACA+TGG + Chr8:73339367-73339386 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 73339292 73339687 73339292 ID=MsG0880046434.01;Name=MsG0880046434.01
Chr8 mRNA 73339292 73339687 73339292 ID=MsG0880046434.01.T01;Parent=MsG0880046434.01;Name=MsG0880046434.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|131
Chr8 exon 73339292 73339687 73339292 ID=MsG0880046434.01.T01:exon:11625;Parent=MsG0880046434.01.T01
Chr8 CDS 73339292 73339687 73339292 ID=MsG0880046434.01.T01:cds;Parent=MsG0880046434.01.T01
Gene Sequence

>MsG0880046434.01.T01

ATGGCTTCCAAGGTTGCTATGCTCCTTTGCCTCAACATCCTATTTTTCACTGTGGTAAGCTCTACATATGTTCCATGTCCCCCACCACCTCACAAAGATCACAGCCACAAACACCCACATCCACCTTCCTCTAAATATCCAACTTGTCCTAGAGACACAATTAAGTTTGGTGTGTGTGCTGATGTATTGGGTTTGATTAATGTTGAGCTTGGTAAGCCACCAAAGACACCATGCTGCTCTCTCATTGATGGTCTTGCTAATCTTGAAGCTGCTGTGTGCCTTTGTACTGCTCTTAAGGCTAATATCTTAGGCATTAACCTTAATCTTCCCATTAATTTGAGCTTGGTTCTTAACTATTGTGGAAAGGGAGTTCCAAAGGGTTTCGTGTGTGCTTAA

Protein sequence

>MsG0880046434.01.T01

MASKVAMLLCLNILFFTVVSSTYVPCPPPPHKDHSHKHPHPPSSKYPTCPRDTIKFGVCADVLGLINVELGKPPKTPCCSLIDGLANLEAAVCLCTALKANILGINLNLPINLSLVLNYCGKGVPKGFVCA*