AlfalfaGEDB | Msa0022000

M. sativa cultivar zhongmu-4 / Msa0022000

KEGG: K08857
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0022000	XP_013467501.1	95.678	995	42	1	1	995	1	994	0.0	1945

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0022000	sp\|Q9LT35\|NEK6_ARATH	72.000	275	76	1	1	275	1	274	7.93e-151	456
Msa0022000	sp\|Q9LT35\|NEK6_ARATH	42.969	128	54	4	880	990	282	407	5.59e-16	85.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0022000	Msa0026450	0.803108	4.080177e-49	-8.615850e-47
Msa0022000	Msa0042550	0.812357	4.415744e-51	-8.615850e-47
Msa0022000	Msa0078640	0.814918	1.206071e-51	-8.615850e-47
Msa0022000	Msa0145260	0.800177	1.628418e-48	-8.615850e-47
Msa0022000	Msa0194440	0.815061	1.120960e-51	-8.615850e-47
Msa0022000	Msa0227340	0.800826	1.201323e-48	-8.615850e-47
Msa0022000	Msa0241140	0.805872	1.082097e-49	-8.615850e-47
Msa0022000	Msa0264830	0.814869	1.236912e-51	-8.615850e-47
Msa0022000	Msa0274640	0.809164	2.166339e-50	-8.615850e-47
Msa0022000	Msa0302870	0.816130	6.479589e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0022000	MtrunA17_Chr1g0173921	95.678	995	42	1	1	995	1	994	0.0	1945
Msa0022000	MtrunA17_Chr7g0258051	71.034	435	109	4	1	432	13	433	0.0	606
Msa0022000	MtrunA17_Chr7g0258051	45.000	260	78	8	778	995	406	642	1.79e-41	162
Msa0022000	MtrunA17_Chr4g0025671	65.371	283	95	1	1	283	5	284	2.99e-132	425
Msa0022000	MtrunA17_Chr7g0260191	64.773	264	93	0	1	264	1	264	1.54e-127	400
Msa0022000	MtrunA17_Chr7g0260191	55.172	58	26	0	928	985	552	609	1.09e-11	68.9
Msa0022000	MtrunA17_Chr1g0190831	61.754	285	98	2	1	285	1	274	4.97e-127	399
Msa0022000	MtrunA17_Chr1g0190831	59.259	54	22	0	932	985	553	606	2.77e-12	70.9
Msa0022000	MtrunA17_Chr2g0321841	66.794	262	87	0	1	262	1	262	6.17e-127	410
Msa0022000	MtrunA17_Chr2g0321841	75.472	53	13	0	933	985	938	990	3.31e-16	84.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0022000	AT3G20860.1	72.000	275	76	1	1	275	1	274	8.06e-152	456
Msa0022000	AT3G20860.1	42.969	128	54	4	880	990	282	407	5.69e-17	85.1
Msa0022000	AT3G12200.1	53.639	371	163	4	1	364	16	384	9.93e-131	407
Msa0022000	AT3G12200.1	58.824	68	28	0	925	992	501	568	1.35e-15	81.6
Msa0022000	AT3G44200.2	66.541	266	89	0	1	266	5	270	1.56e-129	416
Msa0022000	AT3G44200.2	69.811	53	16	0	933	985	883	935	4.89e-14	77.4
Msa0022000	AT3G44200.1	66.541	266	89	0	1	266	5	270	1.56e-129	416
Msa0022000	AT3G44200.1	69.811	53	16	0	933	985	883	935	4.89e-14	77.4
Msa0022000	AT3G12200.2	52.231	381	163	5	1	364	16	394	2.26e-127	399
Msa0022000	AT3G12200.2	58.824	68	28	0	925	992	511	578	1.38e-15	81.6

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Find 175 sgRNAs with CRISPR-Local

Find 502 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTATCAAAACAACCTT+CGG	0.461948	1_1:+40251170	None:intergenic
AAAATCTGAAGCATTCACAG+AGG	0.763262	1_1:-40250284	Msa0022000:CDS
AAACTTGGTAGAGGTGCTTT+AGG	0.338345	1_1:-40257163	Msa0022000:CDS
AAAGAAAATAACATTCGTCT+AGG	0.544830	1_1:-40253150	Msa0022000:intron
AAAGCTGGAAGAGCTTGCTG+CGG	0.664449	1_1:-40250014	Msa0022000:CDS
AAATAGAGCTGTAAGAGACA+TGG	0.605421	1_1:-40251736	Msa0022000:CDS
AAATCTGACATATGGTCGCT+TGG	0.513325	1_1:-40252693	Msa0022000:intron
AAATCTTACAAATCTAGTGG+TGG	0.626268	1_1:-40252026	Msa0022000:CDS
AAATTATCAAGAGCATGCTG+AGG	0.697784	1_1:-40252262	Msa0022000:CDS
AACAATTGGCAGTGGAGAAA+TGG	0.452995	1_1:+40252400	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATAATGAATGAGACTTGA+AGG	+	chr1_1:40249689-40249708	None:intergenic	30.0%
TTCACAAAACTGAAAGAAAG+AGG	-	chr1_1:40250004-40250023	Msa0022000:CDS	30.0%
ATGATGATTTCATTCATGGT+AGG	-	chr1_1:40250262-40250281	Msa0022000:CDS	30.0%
TGTGTTGAAGAAGATTAGAT+TGG	-	chr1_1:40250287-40250306	Msa0022000:CDS	30.0%
TATAAATCAGCATGGATTCA+TGG	+	chr1_1:40250560-40250579	None:intergenic	30.0%
TACATGTCTATAAATCAGCA+TGG	+	chr1_1:40250568-40250587	None:intergenic	30.0%
TAGGATGAAATTTCTACATC+AGG	-	chr1_1:40251208-40251227	Msa0022000:CDS	30.0%
AGGATGAAATTTCTACATCA+GGG	-	chr1_1:40251209-40251228	Msa0022000:CDS	30.0%
CTTGTGTAAAAAACAAGGTA+AGG	+	chr1_1:40251418-40251437	None:intergenic	30.0%
GTCTATAAAAATACGAAGAG+GGG	-	chr1_1:40251462-40251481	Msa0022000:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	40249643	40257506	40249643	ID=Msa0022000;Name=Msa0022000
chr1_1	mRNA	40249643	40257506	40249643	ID=Msa0022000-mRNA-1;Parent=Msa0022000;Name=Msa0022000-mRNA-1;_AED=0.07;_eAED=0.07;_QI=297\|1\|1\|1\|1\|1\|13\|270\|995
chr1_1	exon	40249643	40252156	40249643	ID=Msa0022000-mRNA-1:exon:12157;Parent=Msa0022000-mRNA-1
chr1_1	exon	40252238	40252289	40252238	ID=Msa0022000-mRNA-1:exon:12156;Parent=Msa0022000-mRNA-1
chr1_1	exon	40252386	40252459	40252386	ID=Msa0022000-mRNA-1:exon:12155;Parent=Msa0022000-mRNA-1
chr1_1	exon	40252554	40252603	40252554	ID=Msa0022000-mRNA-1:exon:12154;Parent=Msa0022000-mRNA-1
chr1_1	exon	40252694	40252775	40252694	ID=Msa0022000-mRNA-1:exon:12153;Parent=Msa0022000-mRNA-1
chr1_1	exon	40253009	40253058	40253009	ID=Msa0022000-mRNA-1:exon:12152;Parent=Msa0022000-mRNA-1
chr1_1	exon	40253151	40253193	40253151	ID=Msa0022000-mRNA-1:exon:12151;Parent=Msa0022000-mRNA-1
chr1_1	exon	40253277	40253357	40253277	ID=Msa0022000-mRNA-1:exon:12150;Parent=Msa0022000-mRNA-1
chr1_1	exon	40253915	40253960	40253915	ID=Msa0022000-mRNA-1:exon:12149;Parent=Msa0022000-mRNA-1
chr1_1	exon	40254599	40254645	40254599	ID=Msa0022000-mRNA-1:exon:12148;Parent=Msa0022000-mRNA-1
chr1_1	exon	40254753	40254818	40254753	ID=Msa0022000-mRNA-1:exon:12147;Parent=Msa0022000-mRNA-1
chr1_1	exon	40256799	40256865	40256799	ID=Msa0022000-mRNA-1:exon:12146;Parent=Msa0022000-mRNA-1
chr1_1	exon	40257124	40257506	40257124	ID=Msa0022000-mRNA-1:exon:12145;Parent=Msa0022000-mRNA-1
chr1_1	five_prime_UTR	40257210	40257506	40257210	ID=Msa0022000-mRNA-1:five_prime_utr;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40257124	40257209	40257124	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40256799	40256865	40256799	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40254753	40254818	40254753	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40254599	40254645	40254599	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40253915	40253960	40253915	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40253277	40253357	40253277	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40253151	40253193	40253151	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40253009	40253058	40253009	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40252694	40252775	40252694	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40252554	40252603	40252554	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40252386	40252459	40252386	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40252238	40252289	40252238	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	CDS	40249913	40252156	40249913	ID=Msa0022000-mRNA-1:cds;Parent=Msa0022000-mRNA-1
chr1_1	three_prime_UTR	40249643	40249912	40249643	ID=Msa0022000-mRNA-1:three_prime_utr;Parent=Msa0022000-mRNA-1

Gene Sequence

>Msa0022000

ATGGAAGATTATGAGGTGATAGAGAAACTTGGTAGAGGTGCTTTAGGTGCAACTTTTCTTGTTCTTCACAAAACTGAAAGAAAGAGGTATGTGTTGAAGAAGATTAGATTGGCCAAGCATACAGAGAAGTCCAAATTGACAGCATACCAAGAGCTGGACCTGATCGCACAACTAAATTATCCTTATATTGTGGAGTACAAAGATGCTTGGGTGGAGAAGGAGGACTATATATACATTATAAGCGGCTATTGCGAAGGAGGTGACATGGCTGAGAATATAAAGAAAGCTCGAGGATCTTTCTTTCCAGAGGAGAAAGTCTGCAAATGGATGACTCAGTTGTTGCTAGCTGTGGACTACCTGCACTCTAACCGCGTGCTACACAGAGATCTTAAGTGTTCCAACATATTCCTCACCAAAGAAAATAACATTCGTCTAGGTGACTTTGGTCTTGCAAAGCTACTTGACACAGACGACCCTGCTTCTTCGGTTGTTGGAACTCTAAATTACATGTGTCCTGAGATCTTTTCTGATATGCCTTACGGTTATAAATCTGACATATGGTCGCTTGGTTGCTGCATGTTTGAAATTGTTGCACATCAACCGGCATTTCGTGCTCCAGACAGGGCTGGACTTATCAATAAAATAAATAGATCCACCATTTCTCCACTGCCAATTGTTTACTCTTCCACACTGAAACAAATTATCAAGAGCATGCTGAGGAAAAACCCTGAACACAGACCAACAGCAGCTGAATTGTTGAGGCATCCTCATTTACAACCTTTTGTTCTCCGCTCTCGTGATGCACCCTCTGTTTTTCTTCCGGTACATCTCATAAGTTGTAACTCACCTAAAAAATCTTACAAATCTAGTGGTGGCAAAGACAATAAGGACAGAGAAGAAGGATTAGTTCACCATTTGGAGCGAGTTTATCCCATTGAGGGAAATGGAGACGTACAGACAAGAAACCGACCAAATGGTCGCAAGCTAGCAGTGTCAGTGAGCACAGAAGACAACCTTGAGACTAAAAGGGTTGATCCTACCAGCTACTCATTGGAATTCTCTACAAGTTTTAGTGGCTCAAAAGACGAGTCAACAACATCTGAATCAACCGTTTGCAGCGTGTGCAAGGAAGCAGACTTAAAAAATAGAGCTGTAAGAGACATGGATGATACAGAGATCACTTCAAAGAGTACCCTAGATTTTGCACATGAAAAACAAGTATTTGCTACTGAACATTTTCAGAAGTCTTATGCGGTTGACATAAATACAGGGACTAAAAAAGTTGAAGATACTTTTTCCAATGAAGACAGAAGTGGCAATGATAAAGATGCATCCACCAGTGACGATGTCACATCACAATGCACTCGAGATTCTGTGCACGTAGAAAAAAGATTTACTGTCGAACATTTTCTGAAGGCCGATTCAATTGACATAAATGCATTAACTACAGAAGATAAATACAAGTTTTCCAATAAAGGTTTTGATAAAGCTGAATTACAGAGAGAAGATGTGAAACCTGAGGACTTCATTAAATCAATAATGTCCAGTGAAAATAGATTGGGCATTGATATAGATGGGTCCATTGATGAGGTCACTTCAGAAAGTACACTAGAGTTTTTGCTTGTAGAACAATTTATTACTGAACATTGTCAGAATCCTAATGCATTTGATATAAATGCAGTGACAACAGAAGTTGAAGGTACTTTATCCGAAGGTTGTTTTGATACTTTTTCCGAATGTGATTTCGATGCTTTCTCCGAAACACAGAGAGAAGACGCCAAACCAGATGACTCAAATAAATCAATCATTTACAGGAACGACTGTAATGGAAATGATAAAGAGCCCGTTGATGTTATCACTTCAAAGAGCACACTTGATTCTGTACATGAAAAACAGGAATGTGCCGCTGAGAATTCTCAGAACTCAAATACAATTGGCATAAATACAGAGACTACAAAAGTTGACGATAGTCTTTCTAGTGAAGGTTTTGATAAAGCTGTAGCACAAAGAGAAGATGCCAAACCGGAGGACTCGAGTACTTCAATCATTTACATCAAAGACTGCAATGGAGATGATAAAGAACAGTCCATCGGTGATATCACTTCAAAGAGCACACTGGATTCTTTACATGAAGAACAGGAATGTGCCGCTGAGAATTTTCCAAAGTCAAATACAATTGACATACATCCTGTGACTACAAATGTTGATGATACTCTTTCTGATAAAGGTTTTGATATAGTTGAATTGCAAACAGAGGATGTCAAACCTGAGGACTCAGGCATGTCAATCATTTACCGCAATATCAATGACGAAAAAGAGTCTATTGATGAGATCACATCAAAGAGCACAGCCGATTCTGTGCACGAAGAACGGCAATTTTCTGCTGAACACTTTCATAATCCAGATACAATTGACACAAATGCAGTGACTACAAAAGTTGATGATACTTTTTCCAACGGAGGTTTTGATAAAGTTGAAGCCAGAAGTGACAATGATAAAATTGGTTTCATCAACGAGGATATGGAATCACCGATTGTGCATTCTGTTAGGGTTGAACATGACCCTGATACTGTAAGCTGCACGAAGGAAAGTGAAAAATCTGAAGCATTCACAGAGGACTCACACATGAACATTCTGACTTCTGAGAGTAATGATACACTTCCAACCAAGTATGAAGGCAGTTCCACACATAAACACGATCAAAATGCCATTGTGGATGATAAGACACCAAATGAAATTTCCTTAAGTACAAATATTTCACTAGGGGGTGATAAAACAAAGAGGTTGTTGAAAAATTCCGGCCAACAAAGAGCTGATGCTTTGGAGTCTCTGCTTGAGTTATGTGCACATTTACTTAAGCAAGGAAAGCTGGAAGAGCTTGCTGCGGTGCTAAGACCGTTTGGAGAAGACACAGTTTCATCCAGAGAAACAGCTATATGGCTGACAAAAAGCCTCATGTCGTCACAAAAATTTAATCTGGAAACCTAG

Protein sequence

>Msa0022000

MEDYEVIEKLGRGALGATFLVLHKTERKRYVLKKIRLAKHTEKSKLTAYQELDLIAQLNYPYIVEYKDAWVEKEDYIYIISGYCEGGDMAENIKKARGSFFPEEKVCKWMTQLLLAVDYLHSNRVLHRDLKCSNIFLTKENNIRLGDFGLAKLLDTDDPASSVVGTLNYMCPEIFSDMPYGYKSDIWSLGCCMFEIVAHQPAFRAPDRAGLINKINRSTISPLPIVYSSTLKQIIKSMLRKNPEHRPTAAELLRHPHLQPFVLRSRDAPSVFLPVHLISCNSPKKSYKSSGGKDNKDREEGLVHHLERVYPIEGNGDVQTRNRPNGRKLAVSVSTEDNLETKRVDPTSYSLEFSTSFSGSKDESTTSESTVCSVCKEADLKNRAVRDMDDTEITSKSTLDFAHEKQVFATEHFQKSYAVDINTGTKKVEDTFSNEDRSGNDKDASTSDDVTSQCTRDSVHVEKRFTVEHFLKADSIDINALTTEDKYKFSNKGFDKAELQREDVKPEDFIKSIMSSENRLGIDIDGSIDEVTSESTLEFLLVEQFITEHCQNPNAFDINAVTTEVEGTLSEGCFDTFSECDFDAFSETQREDAKPDDSNKSIIYRNDCNGNDKEPVDVITSKSTLDSVHEKQECAAENSQNSNTIGINTETTKVDDSLSSEGFDKAVAQREDAKPEDSSTSIIYIKDCNGDDKEQSIGDITSKSTLDSLHEEQECAAENFPKSNTIDIHPVTTNVDDTLSDKGFDIVELQTEDVKPEDSGMSIIYRNINDEKESIDEITSKSTADSVHEERQFSAEHFHNPDTIDTNAVTTKVDDTFSNGGFDKVEARSDNDKIGFINEDMESPIVHSVRVEHDPDTVSCTKESEKSEAFTEDSHMNILTSESNDTLPTKYEGSSTHKHDQNAIVDDKTPNEISLSTNISLGGDKTKRLLKNSGQQRADALESLLELCAHLLKQGKLEELAAVLRPFGEDTVSSRETAIWLTKSLMSSQKFNLET*