AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0423860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423860 MtrunA17_Chr3g0125311 97.484 159 4 0 1 159 6 164 1.06e-107 304
Msa0423860 MtrunA17_Chr1g0160151 62.420 157 51 4 1 157 15 163 1.42e-55 172
Msa0423860 MtrunA17_Chr7g0245371 34.568 162 94 4 4 159 20 175 4.89e-22 87.0
Msa0423860 MtrunA17_Chr4g0033601 33.758 157 82 3 4 154 1 141 4.12e-19 78.6
Msa0423860 MtrunA17_Chr8g0351681 37.107 159 80 6 6 156 47 193 3.73e-18 77.4
Msa0423860 MtrunA17_Chr3g0111371 32.484 157 78 5 4 154 1 135 1.07e-17 74.7
Msa0423860 MtrunA17_Chr5g0402331 31.818 154 81 5 8 155 48 183 2.79e-17 75.1
Msa0423860 MtrunA17_Chr1g0161211 35.135 148 84 4 8 155 46 181 1.16e-16 73.6
Msa0423860 MtrunA17_Chr8g0391691 34.247 146 84 5 6 151 34 167 1.34e-16 73.2
Msa0423860 MtrunA17_Chr5g0402361 32.099 162 85 6 1 155 212 355 2.12e-16 74.7
Msa0423860 MtrunA17_Chr5g0402361 30.000 160 90 5 1 155 40 182 1.36e-14 69.7
Msa0423860 MtrunA17_Chr7g0256171 33.962 159 79 4 4 154 1 141 2.43e-16 71.6
Msa0423860 MtrunA17_Chr5g0444171 34.459 148 79 4 9 154 398 529 3.50e-16 74.7
Msa0423860 MtrunA17_Chr1g0169581 33.553 152 79 5 9 154 5 140 4.17e-16 70.9
Msa0423860 MtrunA17_Chr4g0064231 34.615 156 90 3 8 154 64 216 1.10e-15 71.2
Msa0423860 MtrunA17_Chr6g0482731 31.973 147 88 3 8 154 26 160 1.41e-15 70.1
Msa0423860 MtrunA17_Chr4g0057641 32.667 150 88 4 5 154 3 139 2.19e-15 68.9
Msa0423860 MtrunA17_Chr3g0121191 30.921 152 83 5 7 158 92 221 4.15e-15 70.1
Msa0423860 MtrunA17_Chr5g0434951 33.987 153 76 6 8 154 4 137 2.07e-14 66.2
Msa0423860 MtrunA17_Chr4g0057651 32.653 147 86 4 8 154 6 139 2.28e-14 66.2
Msa0423860 MtrunA17_Chr4g0057661 32.667 150 88 4 5 154 3 139 2.38e-14 66.2
Msa0423860 MtrunA17_Chr4g0074811 32.432 148 82 4 9 154 397 528 2.42e-14 69.3
Msa0423860 MtrunA17_Chr7g0245911 33.333 156 87 6 6 154 45 190 2.93e-14 67.0
Msa0423860 MtrunA17_Chr7g0225921 32.237 152 79 5 10 155 8 141 3.11e-14 65.9
Msa0423860 MtrunA17_Chr1g0166281 33.099 142 77 5 17 155 21 147 5.86e-14 65.5
Msa0423860 MtrunA17_Chr3g0098951 32.051 156 79 5 9 154 389 527 1.04e-13 67.4
Msa0423860 MtrunA17_Chr1g0188171 30.263 152 88 4 7 155 11 147 1.06e-13 64.7
Msa0423860 MtrunA17_Chr2g0329371 34.395 157 83 3 4 154 60 202 4.94e-13 63.9
Msa0423860 MtrunA17_Chr8g0392951 32.026 153 79 5 8 154 24 157 5.68e-13 63.2
Msa0423860 MtrunA17_Chr1g0169621 31.548 168 86 6 9 154 5 165 1.33e-12 62.0
Msa0423860 MtrunA17_Chr1g0185001 28.571 147 80 4 16 156 20 147 1.65e-12 61.6
Msa0423860 MtrunA17_Chr8g0387671 30.822 146 87 4 9 154 372 503 1.77e-12 63.9
Msa0423860 MtrunA17_Chr1g0176751 30.201 149 83 3 9 154 430 560 2.70e-12 63.5
Msa0423860 MtrunA17_Chr1g0200601 32.895 152 75 5 9 154 336 466 4.12e-12 62.8
Msa0423860 MtrunA17_Chr5g0440931 26.316 152 94 3 7 155 11 147 7.33e-12 59.7
Msa0423860 MtrunA17_Chr6g0460211 26.316 152 94 3 7 155 11 147 7.41e-12 59.7
Msa0423860 MtrunA17_Chr8g0366401 30.065 153 96 4 7 159 16 157 8.25e-12 60.1
Msa0423860 MtrunA17_Chr7g0254731 26.316 152 94 3 7 155 11 147 1.16e-11 59.3
Msa0423860 MtrunA17_Chr4g0071941 44.444 72 40 0 83 154 74 145 1.18e-11 59.3
Msa0423860 MtrunA17_Chr6g0459231 31.613 155 81 5 7 155 7 142 1.32e-11 58.9
Msa0423860 MtrunA17_Chr5g0397101 29.452 146 89 4 9 154 372 503 1.38e-11 61.6
Msa0423860 MtrunA17_Chr1g0157131 29.452 146 89 3 9 154 380 511 1.47e-11 61.2
Msa0423860 MtrunA17_Chr3g0093271 27.397 146 88 4 13 155 68 198 1.63e-11 59.7
Msa0423860 MtrunA17_Chr4g0044961 29.932 147 89 4 8 154 57 189 1.90e-11 59.7
Msa0423860 MtrunA17_Chr7g0217621 43.056 72 41 0 83 154 126 197 4.34e-11 58.5
Msa0423860 MtrunA17_Chr3g0116151 28.947 152 95 4 3 154 1 139 7.92e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423860 AT1G21550.1 43.226 155 82 3 2 155 4 153 8.94e-36 122
Msa0423860 AT4G20780.1 40.123 162 83 3 4 154 25 183 2.39e-26 99.0
Msa0423860 AT5G44460.1 38.037 163 87 4 4 159 24 179 2.83e-23 90.5
Msa0423860 AT1G66400.1 35.000 160 82 5 5 158 12 155 5.91e-21 84.0
Msa0423860 AT1G24620.1 35.714 154 81 5 8 158 37 175 1.61e-19 80.9
Msa0423860 AT2G43290.1 33.766 154 91 3 4 154 61 206 1.67e-18 79.0
Msa0423860 AT3G59440.1 35.256 156 78 3 5 154 48 186 2.20e-18 78.2
Msa0423860 AT1G05990.1 33.121 157 84 3 4 154 1 142 1.97e-17 74.7
Msa0423860 AT5G37770.1 32.484 157 84 4 5 154 14 155 2.96e-16 72.0
Msa0423860 AT5G37770.2 32.484 157 84 4 5 154 14 155 2.96e-16 72.0
Msa0423860 AT4G03290.1 33.121 157 86 3 4 154 1 144 1.16e-15 70.1
Msa0423860 AT4G04710.3 33.108 148 81 4 9 154 324 455 4.68e-15 71.6
Msa0423860 AT4G04710.2 33.108 148 81 4 9 154 338 469 5.21e-15 71.6
Msa0423860 AT4G04710.1 33.108 148 81 4 9 154 351 482 5.25e-15 71.6
Msa0423860 AT5G17470.1 31.081 148 88 5 9 154 3 138 5.27e-15 68.2
Msa0423860 AT4G04740.4 32.192 146 85 3 9 154 374 505 6.83e-15 71.2
Msa0423860 AT4G04740.6 32.192 146 85 3 9 154 374 505 6.83e-15 71.2
Msa0423860 AT4G04740.5 32.192 146 85 3 9 154 374 505 6.83e-15 71.2
Msa0423860 AT4G04740.3 32.192 146 85 3 9 154 374 505 6.83e-15 71.2
Msa0423860 AT4G04740.1 32.192 146 85 3 9 154 374 505 6.83e-15 71.2
Msa0423860 AT4G04740.7 32.192 146 85 3 9 154 374 505 6.83e-15 71.2
Msa0423860 AT3G51850.1 33.758 157 71 5 8 154 359 492 6.86e-15 71.2
Msa0423860 AT4G04720.2 33.562 146 83 4 9 154 385 516 9.53e-15 70.9
Msa0423860 AT4G04720.1 33.562 146 83 4 9 154 385 516 9.53e-15 70.9
Msa0423860 AT1G76640.1 32.026 153 82 4 8 154 22 158 1.62e-14 67.4
Msa0423860 AT5G19360.1 29.530 149 85 3 9 154 374 505 2.09e-14 70.1
Msa0423860 AT3G07490.1 33.758 157 82 3 4 154 1 141 2.28e-14 66.6
Msa0423860 AT1G32250.1 31.293 147 95 2 8 154 16 156 8.46e-14 65.5
Msa0423860 AT4G04710.4 33.333 150 80 5 9 154 351 484 9.84e-14 68.2
Msa0423860 AT4G04700.1 34.932 146 81 4 9 154 337 468 1.01e-13 68.2
Msa0423860 AT4G21940.2 32.877 146 84 4 9 154 407 538 1.02e-13 68.2
Msa0423860 AT4G21940.1 32.877 146 84 4 9 154 407 538 1.21e-13 67.8
Msa0423860 AT2G36180.1 30.323 155 81 5 9 154 1 137 1.33e-13 64.7
Msa0423860 AT1G73630.1 29.936 157 88 5 2 154 12 150 1.87e-13 64.7
Msa0423860 AT3G51920.1 29.452 146 85 4 16 158 20 150 1.88e-13 64.3
Msa0423860 AT4G04695.1 33.784 148 80 4 9 154 337 468 1.89e-13 67.0
Msa0423860 AT4G12860.1 29.870 154 92 2 4 154 1 141 2.32e-13 64.3
Msa0423860 AT5G42380.1 33.553 152 88 5 4 154 45 184 8.74e-13 63.2
Msa0423860 AT1G35670.1 30.921 152 78 4 9 154 332 462 1.08e-12 65.1
Msa0423860 AT4G09570.1 31.579 152 77 4 9 154 331 461 1.27e-12 64.7
Msa0423860 AT1G18530.1 29.139 151 97 2 9 159 8 148 2.14e-12 61.6
Msa0423860 AT1G66410.1 26.974 152 93 4 7 155 11 147 2.51e-12 61.2
Msa0423860 AT5G37780.1 26.974 152 93 4 7 155 11 147 2.51e-12 61.2
Msa0423860 AT4G14640.1 28.188 149 95 2 7 155 12 148 2.69e-12 61.2
Msa0423860 AT2G15680.1 30.000 150 91 4 5 154 47 182 4.23e-12 61.6
Msa0423860 AT4G23650.1 31.507 146 86 4 9 154 384 515 4.24e-12 63.2
Msa0423860 AT5G21274.1 26.974 152 93 3 7 155 11 147 4.47e-12 60.8
Msa0423860 AT3G47480.1 41.096 73 41 2 83 154 107 178 6.27e-12 60.8
Msa0423860 AT1G50700.1 28.767 146 90 3 9 154 379 510 7.45e-12 62.8
Msa0423860 AT3G43810.1 26.316 152 94 3 7 155 11 147 1.02e-11 59.7
Msa0423860 AT3G43810.2 27.333 150 91 3 13 159 17 151 1.17e-11 60.8
Msa0423860 AT1G18210.2 26.846 149 89 4 8 154 23 153 1.40e-11 59.7
Msa0423860 AT1G18210.1 26.846 149 89 4 8 154 23 153 1.40e-11 59.7
Msa0423860 AT3G20410.2 28.767 146 90 3 9 154 397 528 2.33e-11 61.2
Msa0423860 AT3G20410.1 28.767 146 90 3 9 154 397 528 2.33e-11 61.2
Msa0423860 AT4G14640.2 28.358 134 84 2 22 155 9 130 3.07e-11 58.2
Msa0423860 AT3G56800.1 25.658 152 95 3 7 155 11 147 3.30e-11 58.5
Msa0423860 AT2G41110.1 25.658 152 95 3 7 155 11 147 3.30e-11 58.5
Msa0423860 AT2G27030.1 25.658 152 95 3 7 155 11 147 3.30e-11 58.5
Msa0423860 AT5G23580.1 29.530 149 84 3 9 154 328 458 5.96e-11 60.1
Msa0423860 AT3G50770.1 29.299 157 94 6 5 156 61 205 8.99e-11 58.2
Msa0423860 AT5G37780.4 29.197 137 79 5 22 155 13 134 9.02e-11 57.0

Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTATTATTGCATATTCTT+TGG 0.216223 3_2:-73908804 None:intergenic
TAGAGATTCAAGTTCTTCTA+AGG 0.309731 3_2:-73908836 None:intergenic
GTTGAAGAGACTTGGATTCT+TGG 0.328203 3_2:+73909050 Msa0423860:CDS
GACTTTCAAAGTGTTTGATT+TGG 0.354266 3_2:+73908987 Msa0423860:CDS
ATCAAGCCTACCATCCGAAT+TGG 0.406165 3_2:-73909121 None:intergenic
TTCAAAGTGTTTGATTTGGA+TGG 0.441224 3_2:+73908991 Msa0423860:CDS
GGGATTGTAGAAGTATGATT+CGG 0.454094 3_2:+73909088 Msa0423860:CDS
ATGATACCAATTCGGATGGT+AGG 0.471644 3_2:+73909115 Msa0423860:CDS
TCAACACACACTCAAGCTCT+TGG 0.480809 3_2:-73909032 None:intergenic
TGAGAATGATGAGTTGGAGA+GGG 0.503791 3_2:+73908954 Msa0423860:CDS
GTGTTTGATTTGGATGGAGA+TGG 0.509027 3_2:+73908997 Msa0423860:CDS
GAGAAGAATGAAGAGAATAG+AGG 0.509945 3_2:+73908919 Msa0423860:CDS
CTTGGATGAAAGTAGTGGGA+GGG 0.521104 3_2:+73909068 Msa0423860:CDS
ATGAGAATGATGAGTTGGAG+AGG 0.535268 3_2:+73908953 Msa0423860:CDS
TGAAGATGAGAATGATGAGT+TGG 0.536288 3_2:+73908948 Msa0423860:CDS
GAGTGTGTGTTGAAGAGACT+TGG 0.561588 3_2:+73909042 Msa0423860:CDS
GGATTCTTGGATGAAAGTAG+TGG 0.564575 3_2:+73909063 Msa0423860:CDS
AGAAGAACTTGAATCTCTAG+TGG 0.579031 3_2:+73908840 Msa0423860:CDS
AAGAATGAAGAGAATAGAGG+AGG 0.602155 3_2:+73908922 Msa0423860:CDS
GATTCTTGGATGAAAGTAGT+GGG 0.605078 3_2:+73909064 Msa0423860:CDS
GCACAGAGTTTAGCTCCTCA+AGG 0.608778 3_2:-73908780 None:intergenic
AAGCTAGATACAAATTGTGA+CGG 0.623579 3_2:+73908748 Msa0423860:CDS
TCTTGGATGAAAGTAGTGGG+AGG 0.636199 3_2:+73909067 Msa0423860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTATTATTGCATATTCTT+TGG - chr3_2:73908807-73908826 None:intergenic 20.0%
!! CTTTTACAACTCTATATCAA+AGG + chr3_2:73908897-73908916 Msa0423860:CDS 25.0%
AAGCTAGATACAAATTGTGA+CGG + chr3_2:73908748-73908767 Msa0423860:CDS 30.0%
! TAGAGATTCAAGTTCTTCTA+AGG - chr3_2:73908839-73908858 None:intergenic 30.0%
! GACTTTCAAAGTGTTTGATT+TGG + chr3_2:73908987-73909006 Msa0423860:CDS 30.0%
!! TTCAAAGTGTTTGATTTGGA+TGG + chr3_2:73908991-73909010 Msa0423860:CDS 30.0%
!! TCGGTTTTATGATACCAATT+CGG + chr3_2:73909107-73909126 Msa0423860:CDS 30.0%
! TTTTATGATACCAATTCGGA+TGG + chr3_2:73909111-73909130 Msa0423860:CDS 30.0%
AGAAGAACTTGAATCTCTAG+TGG + chr3_2:73908840-73908859 Msa0423860:CDS 35.0%
GAGAAGAATGAAGAGAATAG+AGG + chr3_2:73908919-73908938 Msa0423860:CDS 35.0%
AAGAATGAAGAGAATAGAGG+AGG + chr3_2:73908922-73908941 Msa0423860:CDS 35.0%
TGAAGATGAGAATGATGAGT+TGG + chr3_2:73908948-73908967 Msa0423860:CDS 35.0%
GATTCTTGGATGAAAGTAGT+GGG + chr3_2:73909064-73909083 Msa0423860:CDS 35.0%
! GGGATTGTAGAAGTATGATT+CGG + chr3_2:73909088-73909107 Msa0423860:CDS 35.0%
! ATGAGAATGATGAGTTGGAG+AGG + chr3_2:73908953-73908972 Msa0423860:CDS 40.0%
! TGAGAATGATGAGTTGGAGA+GGG + chr3_2:73908954-73908973 Msa0423860:CDS 40.0%
!! GTGTTTGATTTGGATGGAGA+TGG + chr3_2:73908997-73909016 Msa0423860:CDS 40.0%
GTTGAAGAGACTTGGATTCT+TGG + chr3_2:73909050-73909069 Msa0423860:CDS 40.0%
GGATTCTTGGATGAAAGTAG+TGG + chr3_2:73909063-73909082 Msa0423860:CDS 40.0%
ATGATACCAATTCGGATGGT+AGG + chr3_2:73909115-73909134 Msa0423860:CDS 40.0%
TCAACACACACTCAAGCTCT+TGG - chr3_2:73909035-73909054 None:intergenic 45.0%
GAGTGTGTGTTGAAGAGACT+TGG + chr3_2:73909042-73909061 Msa0423860:CDS 45.0%
! TCTTGGATGAAAGTAGTGGG+AGG + chr3_2:73909067-73909086 Msa0423860:CDS 45.0%
! CTTGGATGAAAGTAGTGGGA+GGG + chr3_2:73909068-73909087 Msa0423860:CDS 45.0%
ATCAAGCCTACCATCCGAAT+TGG - chr3_2:73909124-73909143 None:intergenic 45.0%
! GGATGGTAGGCTTGATTTTC+AGG + chr3_2:73909128-73909147 Msa0423860:CDS 45.0%
!! TGACGGTTTTGTGAGCCTTG+AGG + chr3_2:73908765-73908784 Msa0423860:CDS 50.0%
! GCACAGAGTTTAGCTCCTCA+AGG - chr3_2:73908783-73908802 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 73908706 73909185 73908706 ID=Msa0423860;Name=Msa0423860
chr3_2 mRNA 73908706 73909185 73908706 ID=Msa0423860-mRNA-1;Parent=Msa0423860;Name=Msa0423860-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|1|1|0|159
chr3_2 exon 73908706 73909185 73908706 ID=Msa0423860-mRNA-1:exon:18599;Parent=Msa0423860-mRNA-1
chr3_2 CDS 73908706 73909185 73908706 ID=Msa0423860-mRNA-1:cds;Parent=Msa0423860-mRNA-1
Gene Sequence

>Msa0423860

ATGTCTATTCTATCTAGCACTGATTTGCATCGTATTTTTGAGAAGCTAGATACAAATTGTGACGGTTTTGTGAGCCTTGAGGAGCTAAACTCTGTGCTCCAAAGAATATGCAATAATAGTTCACAATTTACCTTAGAAGAACTTGAATCTCTAGTGGAAAAGAAGAGCTTAGACTTCAATGAATTCTTGTTCTTTTACAACTCTATATCAAAGGAGAAGAATGAAGAGAATAGAGGAGGTGATGAAGATGAGAATGATGAGTTGGAGAGGGATCTTGTGAAGACTTTCAAAGTGTTTGATTTGGATGGAGATGGATTCATTACAAGCCAAGAGCTTGAGTGTGTGTTGAAGAGACTTGGATTCTTGGATGAAAGTAGTGGGAGGGATTGTAGAAGTATGATTCGGTTTTATGATACCAATTCGGATGGTAGGCTTGATTTTCAGGAATTCAAGAACATGATGTTGATTACAAGTGTATGA

Protein sequence

>Msa0423860

MSILSSTDLHRIFEKLDTNCDGFVSLEELNSVLQRICNNSSQFTLEELESLVEKKSLDFNEFLFFYNSISKEKNEENRGGDEDENDELERDLVKTFKVFDLDGDGFITSQELECVLKRLGFLDESSGRDCRSMIRFYDTNSDGRLDFQEFKNMMLITSV*