AlfalfaGEDB | Msa1023950

M. sativa cultivar zhongmu-4 / Msa1023950

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023950	XP_024626487.1	82.730	359	32	1	1	329	1	359	0.0	596

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023950	A0A072U1T4	82.730	359	32	1	1	329	1	359	0.0	596

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002460	Msa1023950	0.803612	3.208786e-49	-8.615850e-47
Msa0003890	Msa1023950	0.842797	2.076179e-58	-8.615850e-47
Msa0006110	Msa1023950	0.850461	1.673505e-60	-8.615850e-47
Msa0009180	Msa1023950	0.821772	3.379916e-53	-8.615850e-47
Msa0011170	Msa1023950	0.819952	8.861785e-53	-8.615850e-47
Msa0014710	Msa1023950	0.823130	1.634921e-53	-8.615850e-47
Msa0015390	Msa1023950	0.830879	2.289674e-55	-8.615850e-47
Msa0015610	Msa1023950	0.810839	9.442732e-51	-8.615850e-47
Msa0017510	Msa1023950	0.806010	1.012524e-49	-8.615850e-47
Msa0020420	Msa1023950	0.822177	2.724012e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023950	MtrunA17_Chr7g0241661	82.730	359	32	1	1	329	1	359	0.0	596
Msa1023950	MtrunA17_Chr7g0241681	53.719	363	129	4	1	325	2	363	2.63e-118	345
Msa1023950	MtrunA17_Chr7g0241711	40.773	233	103	3	1	206	1	225	7.65e-34	124
Msa1023950	MtrunA17_Chr7g0241701	64.384	73	26	0	142	214	1	73	1.31e-26	101
Msa1023950	MtrunA17_Chr7g0241941	33.523	176	79	2	69	206	9	184	1.76e-25	101
Msa1023950	MtrunA17_Chr7g0248041	28.000	225	132	6	111	307	312	534	1.51e-12	68.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023950	AT5G04110.1	32.432	185	89	3	165	313	340	524	2.07e-22	98.2
Msa1023950	AT5G04110.3	32.432	185	89	3	165	313	340	524	2.07e-22	98.2
Msa1023950	AT5G04110.2	32.432	185	89	3	165	313	341	525	2.10e-22	98.2
Msa1023950	AT5G04110.4	32.121	165	76	3	165	293	340	504	9.08e-17	81.3

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Find 54 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCCAAAGATCAAAGA+AGG	0.541347	7_1:+52292194	Msa1023950:CDS
AACTGCACCTAAGGGAGATC+TGG	0.441097	7_1:-52292935	None:intergenic
AAGAATATGCTTTAAAATCA+TGG	0.406708	7_1:+52292746	Msa1023950:CDS
ACTTACCATCATTTATGTAT+TGG	0.365830	7_1:-52292126	None:intergenic
AGCTAGAGAATGCTTAAAAT+CGG	0.441855	7_1:+52292681	Msa1023950:CDS
AGCTTGAAACCTAGGTCCAA+TGG	0.530844	7_1:-52292551	None:intergenic
ATAGAGACCAGATCTCCCTT+AGG	0.562925	7_1:+52292928	Msa1023950:CDS
ATCGGTAAAGAGAAATATAG+TGG	0.522648	7_1:+52292169	Msa1023950:CDS
ATGATAAAGATACGATGATA+TGG	0.421547	7_1:+52289163	Msa1023950:exon
ATGCTCGTCATAGATTGGGA+TGG	0.629267	7_1:-52292862	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATAATGTATACATACCTG+TGG	-	chr7_1:52289353-52289372	None:intergenic	30.0%
GATATACCAAATACATCATG+CGG	+	chr7_1:52289408-52289427	Msa1023950:intron	30.0%
TTAAAAACAAAGTAACGGTG+TGG	-	chr7_1:52289513-52289532	None:intergenic	30.0%
ACCTCAAAGAAAAATTGACT+AGG	+	chr7_1:52289653-52289672	Msa1023950:intron	30.0%
TAATGGTTCAATTTCTTGGT+TGG	+	chr7_1:52289778-52289797	Msa1023950:intron	30.0%
AAAAAAGGCCACAATGTATT+TGG	+	chr7_1:52289986-52290005	Msa1023950:intron	30.0%
ACTTCTCAAGATAACAATTG+AGG	-	chr7_1:52290995-52291014	None:intergenic	30.0%
AAAGATATGCATGCCAAAAT+TGG	+	chr7_1:52291363-52291382	Msa1023950:intron	30.0%
TTGAGATGATTTCAAAGGTA+AGG	-	chr7_1:52291889-52291908	None:intergenic	30.0%
ATCGGTAAAGAGAAATATAG+TGG	+	chr7_1:52292169-52292188	Msa1023950:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	52289111	52292981	52289111	ID=Msa1023950;Name=Msa1023950
chr7_1	mRNA	52289111	52292981	52289111	ID=Msa1023950-mRNA-1;Parent=Msa1023950;Name=Msa1023950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=66\|1\|0.5\|1\|1\|1\|2\|0\|329
chr7_1	exon	52289111	52289296	52289111	ID=Msa1023950-mRNA-1:exon:10375;Parent=Msa1023950-mRNA-1
chr7_1	exon	52292112	52292981	52292112	ID=Msa1023950-mRNA-1:exon:10376;Parent=Msa1023950-mRNA-1
chr7_1	five_prime_UTR	52289111	52289176	52289111	ID=Msa1023950-mRNA-1:five_prime_utr;Parent=Msa1023950-mRNA-1
chr7_1	CDS	52289177	52289296	52289177	ID=Msa1023950-mRNA-1:cds;Parent=Msa1023950-mRNA-1
chr7_1	CDS	52292112	52292981	52292112	ID=Msa1023950-mRNA-1:cds;Parent=Msa1023950-mRNA-1

Gene Sequence

>Msa1023950

ATGATATGGTCAAAAGATATAGCTTTCAAACCTTGTGAGGAGAAGTCATCACATCATATTCTTCAAAATCAAAAGCACAAAGATCAAGTGCTTTTGAATTCTAGTTCCAAAAATCCACAGAAGAGGAAATTTAACCAATACATAAATGATGGTAAGTGCGCTACTAGTTTTGAACTCATCGGTAAAGAGAAATATAGTGGTGAAAATGCCAAAGATCAAAGAAGGAACTCATCTTATCCTCTATCATGTTCTGCACTATCGGATGGAACACAAGAATATGTTCCCCCTTCTCTAAATGACAATGCAAAGTTTTGTGGTATTGCTACACAAGACTGCTCTCACCTAAGTGACTTTGATGATTCTATTAAGGGTTCTAGTTACCTTGTGAATCAAGATAGCTTTAATATCCATGCTAGAGATTTAATGGCGAGTAATCGATATTTGTATGGTATCGAGACATCAATCGAGGAGAAATCAAAAGAAGAAGACATGGAAGTCATTAACTCACTCAAGAACTTTTTACCTTCTAGGGGTAACCATCTTCCAAGACCAGTTATTCCCATTGGACCTAGGTTTCAAGCTGAAGTTCGTAAATGGGATGGCAAAGCCAACATGAAACATGCTATGCTCAACTCATGCACGTGTGACAATCCTGAATTATTTAATCGTGTTAAAAAACGCATCGGTGAAGCTAGAGAATGCTTAAAATCGGAGATTGATACCGGTTTTTCAAGTCGGAAGTTTGATGACATAGAAGAATATGCTTTAAAATCATGGACAGTGGAAGAGCAAAAGAAATTTGAATCTCTAAAAAAATTGAATTTGCTATCAAGTTACACCAAATTTTCGAAGCTCGTCATGGAGTACTTTCCATCCCAATCTATGACGAGCATGATGAATTACTATTATAATGTATACATCCCTAGATGTATGAGTATAGAGACCAGATCTCCCTTAGGTGCAGTTGATCGTGAATCAAAATTGAACTAG

Protein sequence

>Msa1023950

MIWSKDIAFKPCEEKSSHHILQNQKHKDQVLLNSSSKNPQKRKFNQYINDGKCATSFELIGKEKYSGENAKDQRRNSSYPLSCSALSDGTQEYVPPSLNDNAKFCGIATQDCSHLSDFDDSIKGSSYLVNQDSFNIHARDLMASNRYLYGIETSIEEKSKEEDMEVINSLKNFLPSRGNHLPRPVIPIGPRFQAEVRKWDGKANMKHAMLNSCTCDNPELFNRVKKRIGEARECLKSEIDTGFSSRKFDDIEEYALKSWTVEEQKKFESLKKLNLLSSYTKFSKLVMEYFPSQSMTSMMNYYYNVYIPRCMSIETRSPLGAVDRESKLN*