AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0084300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0084300 MtrunA17_Chr1g0199961 98.233 283 5 0 1 283 1 283 0.0 576
Msa0084300 MtrunA17_Chr6g0462061 74.912 283 71 0 1 283 1 283 7.49e-163 453
Msa0084300 MtrunA17_Chr4g0030021 44.086 279 140 3 3 265 2 280 4.41e-76 233
Msa0084300 MtrunA17_Chr4g0073251 45.000 260 128 4 3 248 2 260 2.60e-73 225
Msa0084300 MtrunA17_Chr8g0366571 43.182 264 140 3 4 257 3 266 6.74e-68 212
Msa0084300 MtrunA17_Chr8g0389221 38.636 264 150 4 5 256 4 267 2.61e-58 187
Msa0084300 MtrunA17_Chr8g0386471 34.661 251 154 2 12 252 13 263 5.29e-58 186
Msa0084300 MtrunA17_Chr5g0411131 41.065 263 145 3 5 257 4 266 5.23e-53 174
Msa0084300 MtrunA17_Chr4g0062101 34.749 259 156 3 2 247 4 262 8.10e-53 173
Msa0084300 MtrunA17_Chr5g0447681 27.273 253 153 4 3 243 48 281 4.88e-25 101
Msa0084300 MtrunA17_Chr2g0307651 36.986 146 92 0 1 146 1 146 8.80e-24 95.1
Msa0084300 MtrunA17_Chr2g0307641 48.052 77 38 1 185 259 17 93 6.15e-19 79.7
Msa0084300 MtrunA17_Chr5g0447671 33.333 117 65 3 120 232 49 156 8.65e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0084300 AT3G12090.1 67.254 284 90 2 1 283 1 282 1.69e-131 374
Msa0084300 AT4G23410.1 56.940 281 119 2 1 280 1 280 3.50e-116 335
Msa0084300 AT3G45600.1 44.803 279 138 3 3 265 2 280 8.21e-84 253
Msa0084300 AT4G28050.1 41.538 260 140 3 1 248 1 260 1.62e-77 236
Msa0084300 AT4G30430.1 38.148 270 155 3 1 258 1 270 1.54e-75 232
Msa0084300 AT2G23810.1 39.773 264 146 4 1 251 1 264 1.56e-75 232
Msa0084300 AT5G60220.1 43.321 277 141 4 3 263 2 278 3.48e-65 207
Msa0084300 AT1G18520.1 40.075 267 142 4 1 250 1 266 1.56e-62 198
Msa0084300 AT5G46700.1 42.045 264 143 3 4 257 3 266 6.62e-61 194
Msa0084300 AT2G19580.1 40.377 265 147 4 4 257 3 267 6.10e-58 187
Msa0084300 AT1G63260.2 35.317 252 147 4 5 246 6 251 7.56e-56 181
Msa0084300 AT1G63260.6 34.167 240 148 3 5 234 6 245 1.14e-51 171
Msa0084300 AT1G63260.1 34.167 240 148 3 5 234 6 245 1.14e-51 171
Msa0084300 AT1G63260.3 34.167 240 148 3 5 234 6 245 1.37e-51 171
Msa0084300 AT1G63260.5 35.211 213 128 3 5 207 6 218 1.35e-45 153
Msa0084300 AT3G45600.2 50.340 147 71 1 3 147 2 148 5.51e-45 151
Msa0084300 AT1G63260.4 36.782 174 108 2 5 176 6 179 1.72e-37 132
Msa0084300 AT2G03840.1 27.848 237 155 4 12 232 23 259 2.39e-32 120
Msa0084300 AT5G23030.1 31.128 257 166 4 1 247 1 256 2.92e-31 117
Msa0084300 AT5G57810.1 26.459 257 140 9 10 233 60 300 8.76e-19 85.1

Find 70 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATTGGGTTTGACTATATT+TGG 0.153305 1_2:-63661857 Msa0084300:CDS
GAATACAAAATCCACGTTAT+TGG 0.203585 1_2:-63661739 Msa0084300:CDS
CATAGTCCTGCTCCAATTAT+TGG 0.212597 1_2:+63662046 None:intergenic
CAAACACCACTTCTTGTAAT+AGG 0.226966 1_2:-63661986 Msa0084300:CDS
TTAGCATCCATACCAATAAT+TGG 0.238729 1_2:-63662058 Msa0084300:CDS
ATTATAATTTGTACCTGTAT+TGG 0.306319 1_2:+63661626 None:intergenic
GGTTTGACTATATTTGGTTT+TGG 0.337933 1_2:-63661851 Msa0084300:CDS
GTTACTAATAGTAGCACTAT+TGG 0.340814 1_2:-63661873 Msa0084300:CDS
CAATAATTGGAGCAGGACTA+TGG 0.354102 1_2:-63662045 Msa0084300:CDS
CTCATAGGGATTTATTCAAT+TGG 0.355629 1_2:-63661286 Msa0084300:CDS
TTACTAATAGTAGCACTATT+GGG 0.365406 1_2:-63661872 Msa0084300:CDS
GGTGGAGATGGTTGCATGAT+AGG 0.367982 1_2:-63660507 Msa0084300:CDS
CACTCATAACATAGCAATCT+TGG 0.369072 1_2:+63661394 None:intergenic
AACTGCATGCAATTACAATA+TGG 0.369952 1_2:-63661467 Msa0084300:CDS
TGCTTGGGTGGGTGCTCCAT+AGG 0.377208 1_2:-63660443 Msa0084300:three_prime_UTR
ACTTTCCTCTTGGACCCCTT+TGG 0.378547 1_2:-63661672 Msa0084300:CDS
CTTGTGACAAACTTTCCTCT+TGG 0.384482 1_2:-63661682 Msa0084300:CDS
CTATTACAAGAAGTGGTGTT+TGG 0.407257 1_2:+63661987 None:intergenic
AGGTTCAGTAACACAGTGAT+AGG 0.407586 1_2:-63662103 Msa0084300:CDS
TAAGAGTGAGAGAAATGTAT+AGG 0.412153 1_2:-63662123 Msa0084300:exon
TTACCAATGGTAATCCCATC+TGG 0.428713 1_2:+63661186 None:intergenic
TTCATGTTGCATGTGCACTA+TGG 0.430443 1_2:-63661916 Msa0084300:CDS
TTGAAGACATAAGAAGGAAT+TGG 0.432488 1_2:-63661348 Msa0084300:CDS
GCTGAAACAGATTATCCATA+TGG 0.446295 1_2:-63661235 Msa0084300:CDS
ATATCATTAGCTGGTTTCAT+TGG 0.450232 1_2:-63661947 Msa0084300:CDS
AGTTCCACAGTGTATGGTAT+TGG 0.468810 1_2:-63660403 Msa0084300:three_prime_UTR
GAATGATATGTCTCCAATAC+AGG 0.472664 1_2:-63661639 Msa0084300:intron
TATGCTCTATCATCCATGCT+TGG 0.472740 1_2:-63660459 Msa0084300:three_prime_UTR
TTCACTAGTTCCACAGTGTA+TGG 0.473795 1_2:-63660409 Msa0084300:three_prime_UTR
TCCTGCTCCAATTATTGGTA+TGG 0.474090 1_2:+63662051 None:intergenic
GCTTGGGTGGGTGCTCCATA+GGG 0.477808 1_2:-63660442 Msa0084300:three_prime_UTR
CATTCTGCATATAATCCAAA+GGG 0.480639 1_2:+63661657 None:intergenic
TACTAGGAAGCTGATGATGC+TGG 0.483715 1_2:+63660344 None:intergenic
TGGGTCTAACTTTGGTCATA+CGG 0.487577 1_2:+63661206 None:intergenic
AAGACTCAGATTGCTACAAA+TGG 0.488256 1_2:-63661426 Msa0084300:CDS
GTACTTGTGATATCATTAGC+TGG 0.488688 1_2:-63661956 Msa0084300:CDS
ATGCTCTATCATCCATGCTT+GGG 0.490576 1_2:-63660458 Msa0084300:three_prime_UTR
ATCCCATCTGGGTCTAACTT+TGG 0.490692 1_2:+63661198 None:intergenic
CTCTATCATCCATGCTTGGG+TGG 0.491952 1_2:-63660455 Msa0084300:three_prime_UTR
ATAAAACCTATTACAAGAAG+TGG 0.520418 1_2:+63661980 None:intergenic
GTGAATTGTCACAATCCCTA+TGG 0.524902 1_2:+63660427 None:intergenic
GACCCAGATGGGATTACCAT+TGG 0.527130 1_2:-63661189 Msa0084300:intron
TTGTAATTGCATGCAGTTGG+TGG 0.535222 1_2:+63661472 None:intergenic
TCCATACCAATAATTGGAGC+AGG 0.539612 1_2:-63662052 Msa0084300:CDS
ATGTGCACTATGGTTGTACT+TGG 0.544797 1_2:-63661906 Msa0084300:CDS
ATCTTACAGATTATTCACCT+TGG 0.548050 1_2:-63661772 Msa0084300:CDS
ATGGAGCACCCACCCAAGCA+TGG 0.563143 1_2:+63660446 None:intergenic
ATATTGTAATTGCATGCAGT+TGG 0.563181 1_2:+63661469 None:intergenic
GGTTCAGTAACACAGTGATA+GGG 0.566697 1_2:-63662102 Msa0084300:CDS
GACCAAAGTTAGACCCAGAT+GGG 0.574022 1_2:-63661200 Msa0084300:CDS
GAGTGTGATTCATGCAAAGC+TGG 0.574954 1_2:-63661376 Msa0084300:CDS
CCCTATGAGCAAAATAAGCA+TGG 0.576576 1_2:+63661300 None:intergenic
TACCAATGGTAATCCCATCT+GGG 0.577451 1_2:+63661187 None:intergenic
GGTGTGACTAGTAAAGGTGG+TGG 0.578149 1_2:-63661830 Msa0084300:CDS
GAGCAGGACTATGGATGGCA+AGG 0.579423 1_2:-63662036 Msa0084300:CDS
TCATTCTGCATATAATCCAA+AGG 0.583877 1_2:+63661656 None:intergenic
TGACCAAAGTTAGACCCAGA+TGG 0.584683 1_2:-63661201 Msa0084300:CDS
GTGTGCTTGAAGACATAAGA+AGG 0.590298 1_2:-63661354 Msa0084300:CDS
GAGATGGTTGCATGATAGGA+AGG 0.591262 1_2:-63660503 Msa0084300:CDS
TCTATCATCCATGCTTGGGT+GGG 0.595712 1_2:-63660454 Msa0084300:three_prime_UTR
ACATAAAATTGAATGTCTCA+AGG 0.597731 1_2:-63660380 Msa0084300:three_prime_UTR
AATTGGAGCAGGACTATGGA+TGG 0.601063 1_2:-63662041 Msa0084300:CDS
TTTGGTGTGACTAGTAAAGG+TGG 0.610196 1_2:-63661833 Msa0084300:CDS
ATGATGCTGGAAAGAAACAA+AGG 0.616696 1_2:+63660357 None:intergenic
GGTGGTGGTGTAGAAGTGCC+TGG 0.625849 1_2:-63661815 Msa0084300:CDS
TTTATAGTATTCCAATAACG+TGG 0.646190 1_2:+63661728 None:intergenic
ATAATCCAAAGGGGTCCAAG+AGG 0.661558 1_2:+63661667 None:intergenic
TACTCACTATAAGATCTACC+AGG 0.665561 1_2:+63661797 None:intergenic
ATGTCCAATACCATACACTG+TGG 0.699729 1_2:+63660399 None:intergenic
ATTCTGCATATAATCCAAAG+GGG 0.741377 1_2:+63661658 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAATACCTTTTTTT+AGG - chr1_2:63661385-63661404 Msa0084300:CDS 10.0%
!!! TTTTTAAATACCTTTTTTTA+GGG - chr1_2:63661386-63661405 Msa0084300:CDS 10.0%
!!! TTTTTTAAATACCTTTTTTT+AGG - chr1_2:63661385-63661404 Msa0084300:CDS 10.0%
!!! TTTTTAAATACCTTTTTTTA+GGG - chr1_2:63661386-63661405 Msa0084300:CDS 10.0%
!!! ACTATAAAGAATTGTATTTT+AGG - chr1_2:63660695-63660714 Msa0084300:intron 15.0%
!!! CTATAAAGAATTGTATTTTA+GGG - chr1_2:63660696-63660715 Msa0084300:intron 15.0%
!!! ACATTCAAAAAATGTTATTT+TGG + chr1_2:63660867-63660886 None:intergenic 15.0%
!!! TAACTTTTCATCATATATAT+TGG + chr1_2:63661250-63661269 None:intergenic 15.0%
!! GAATAAAAAAAGTTATACTT+TGG + chr1_2:63661691-63661710 None:intergenic 15.0%
!!! AATGTATAAATTTCTTTTCA+TGG - chr1_2:63661711-63661730 Msa0084300:CDS 15.0%
!! ATATTTCAAATGATCTAAAT+CGG - chr1_2:63661745-63661764 Msa0084300:CDS 15.0%
!!! TATATATTTTTACCATTTCT+TGG + chr1_2:63661829-63661848 None:intergenic 15.0%
!!! ACTATAAAGAATTGTATTTT+AGG - chr1_2:63660695-63660714 Msa0084300:intron 15.0%
!!! CTATAAAGAATTGTATTTTA+GGG - chr1_2:63660696-63660715 Msa0084300:intron 15.0%
!!! ACATTCAAAAAATGTTATTT+TGG + chr1_2:63660867-63660886 None:intergenic 15.0%
!!! TAACTTTTCATCATATATAT+TGG + chr1_2:63661250-63661269 None:intergenic 15.0%
!! GAATAAAAAAAGTTATACTT+TGG + chr1_2:63661691-63661710 None:intergenic 15.0%
!!! AATGTATAAATTTCTTTTCA+TGG - chr1_2:63661711-63661730 Msa0084300:CDS 15.0%
!! ATATTTCAAATGATCTAAAT+CGG - chr1_2:63661745-63661764 Msa0084300:CDS 15.0%
!!! TATATATTTTTACCATTTCT+TGG + chr1_2:63661829-63661848 None:intergenic 15.0%
!!! ATTATAATTTGTACCTGTAT+TGG + chr1_2:63660785-63660804 None:intergenic 20.0%
!! AATTGATTAATGCTAAGTTA+TGG - chr1_2:63660889-63660908 Msa0084300:intron 20.0%
!! CATAATTATTGATAAGCTTA+TGG - chr1_2:63661349-63661368 Msa0084300:CDS 20.0%
!! GTATTTAAATCCCTAAAAAA+AGG + chr1_2:63661399-63661418 None:intergenic 20.0%
!!! AAAAAACATCCTTTTTGTAA+AGG - chr1_2:63661453-63661472 Msa0084300:CDS 20.0%
!!! AAAAACATCCTTTTTGTAAA+GGG - chr1_2:63661454-63661473 Msa0084300:CDS 20.0%
!! TATTTCAAATGATCTAAATC+GGG - chr1_2:63661746-63661765 Msa0084300:CDS 20.0%
!!! ATTATAATTTGTACCTGTAT+TGG + chr1_2:63660785-63660804 None:intergenic 20.0%
!! AATTGATTAATGCTAAGTTA+TGG - chr1_2:63660889-63660908 Msa0084300:intron 20.0%
!! CATAATTATTGATAAGCTTA+TGG - chr1_2:63661349-63661368 Msa0084300:CDS 20.0%
!! GTATTTAAATCCCTAAAAAA+AGG + chr1_2:63661399-63661418 None:intergenic 20.0%
!!! AAAAAACATCCTTTTTGTAA+AGG - chr1_2:63661453-63661472 Msa0084300:CDS 20.0%
!!! AAAAACATCCTTTTTGTAAA+GGG - chr1_2:63661454-63661473 Msa0084300:CDS 20.0%
!! TATTTCAAATGATCTAAATC+GGG - chr1_2:63661746-63661765 Msa0084300:CDS 20.0%
! ATAAAACCTATTACAAGAAG+TGG + chr1_2:63660431-63660450 None:intergenic 25.0%
! TTACTAATAGTAGCACTATT+GGG - chr1_2:63660536-63660555 Msa0084300:intron 25.0%
! TTTATAGTATTCCAATAACG+TGG + chr1_2:63660683-63660702 None:intergenic 25.0%
! ATATATATTGGACTTACCAA+TGG + chr1_2:63661238-63661257 None:intergenic 25.0%
! CCTGAGTAAATCTAATTTAT+AGG + chr1_2:63661279-63661298 None:intergenic 25.0%
! CCTATAAATTAGATTTACTC+AGG - chr1_2:63661276-63661295 Msa0084300:CDS 25.0%
! TCAATGACATTAAACTACTT+GGG + chr1_2:63661434-63661453 None:intergenic 25.0%
! TTCAATGACATTAAACTACT+TGG + chr1_2:63661435-63661454 None:intergenic 25.0%
!! AAAACATCCTTTTTGTAAAG+GGG - chr1_2:63661455-63661474 Msa0084300:CDS 25.0%
!!! ACTATTTTTTTTACCGAATG+AGG + chr1_2:63661495-63661514 None:intergenic 25.0%
! ACAATGAAACTTAACTTAGT+TGG + chr1_2:63661582-63661601 None:intergenic 25.0%
! TTTATACAAGAACCAAGAAA+TGG - chr1_2:63661814-63661833 Msa0084300:CDS 25.0%
! ACATAAAATTGAATGTCTCA+AGG - chr1_2:63662028-63662047 Msa0084300:CDS 25.0%
! ATAAAACCTATTACAAGAAG+TGG + chr1_2:63660431-63660450 None:intergenic 25.0%
! TTACTAATAGTAGCACTATT+GGG - chr1_2:63660536-63660555 Msa0084300:intron 25.0%
! TTTATAGTATTCCAATAACG+TGG + chr1_2:63660683-63660702 None:intergenic 25.0%
! ATATATATTGGACTTACCAA+TGG + chr1_2:63661238-63661257 None:intergenic 25.0%
! CCTGAGTAAATCTAATTTAT+AGG + chr1_2:63661279-63661298 None:intergenic 25.0%
! CCTATAAATTAGATTTACTC+AGG - chr1_2:63661276-63661295 Msa0084300:CDS 25.0%
! TCAATGACATTAAACTACTT+GGG + chr1_2:63661434-63661453 None:intergenic 25.0%
! TTCAATGACATTAAACTACT+TGG + chr1_2:63661435-63661454 None:intergenic 25.0%
!! AAAACATCCTTTTTGTAAAG+GGG - chr1_2:63661455-63661474 Msa0084300:CDS 25.0%
!!! ACTATTTTTTTTACCGAATG+AGG + chr1_2:63661495-63661514 None:intergenic 25.0%
! ACAATGAAACTTAACTTAGT+TGG + chr1_2:63661582-63661601 None:intergenic 25.0%
! TTTATACAAGAACCAAGAAA+TGG - chr1_2:63661814-63661833 Msa0084300:CDS 25.0%
! ACATAAAATTGAATGTCTCA+AGG - chr1_2:63662028-63662047 Msa0084300:CDS 25.0%
TAAGAGTGAGAGAAATGTAT+AGG - chr1_2:63660285-63660304 Msa0084300:three_prime_UTR 30.0%
TTAGCATCCATACCAATAAT+TGG - chr1_2:63660350-63660369 Msa0084300:three_prime_UTR 30.0%
! ATATCATTAGCTGGTTTCAT+TGG - chr1_2:63660461-63660480 Msa0084300:three_prime_UTR 30.0%
GTTACTAATAGTAGCACTAT+TGG - chr1_2:63660535-63660554 Msa0084300:intron 30.0%
CTATTGGGTTTGACTATATT+TGG - chr1_2:63660551-63660570 Msa0084300:intron 30.0%
!! GGTTTGACTATATTTGGTTT+TGG - chr1_2:63660557-63660576 Msa0084300:intron 30.0%
ATCTTACAGATTATTCACCT+TGG - chr1_2:63660636-63660655 Msa0084300:intron 30.0%
! TGTATTCTTTTCTTCAACCA+AGG + chr1_2:63660656-63660675 None:intergenic 30.0%
GAATACAAAATCCACGTTAT+TGG - chr1_2:63660669-63660688 Msa0084300:intron 30.0%
ATTCTGCATATAATCCAAAG+GGG + chr1_2:63660753-63660772 None:intergenic 30.0%
CATTCTGCATATAATCCAAA+GGG + chr1_2:63660754-63660773 None:intergenic 30.0%
TCATTCTGCATATAATCCAA+AGG + chr1_2:63660755-63660774 None:intergenic 30.0%
AAGTTATGGTTAATGTAGTC+AGG - chr1_2:63660903-63660922 Msa0084300:intron 30.0%
ATATTGTAATTGCATGCAGT+TGG + chr1_2:63660942-63660961 None:intergenic 30.0%
AACTGCATGCAATTACAATA+TGG - chr1_2:63660941-63660960 Msa0084300:intron 30.0%
TTGAAGACATAAGAAGGAAT+TGG - chr1_2:63661060-63661079 Msa0084300:intron 30.0%
CTCATAGGGATTTATTCAAT+TGG - chr1_2:63661122-63661141 Msa0084300:intron 30.0%
CAATGACATTAAACTACTTG+GGG + chr1_2:63661433-63661452 None:intergenic 30.0%
TATTGATCCCCTTTACAAAA+AGG + chr1_2:63661465-63661484 None:intergenic 30.0%
!!! TGTTATGTGCTTTTTTCAAC+AGG - chr1_2:63661880-63661899 Msa0084300:CDS 30.0%
TAAGAGTGAGAGAAATGTAT+AGG - chr1_2:63660285-63660304 Msa0084300:three_prime_UTR 30.0%
TTAGCATCCATACCAATAAT+TGG - chr1_2:63660350-63660369 Msa0084300:three_prime_UTR 30.0%
! ATATCATTAGCTGGTTTCAT+TGG - chr1_2:63660461-63660480 Msa0084300:three_prime_UTR 30.0%
GTTACTAATAGTAGCACTAT+TGG - chr1_2:63660535-63660554 Msa0084300:intron 30.0%
CTATTGGGTTTGACTATATT+TGG - chr1_2:63660551-63660570 Msa0084300:intron 30.0%
!! GGTTTGACTATATTTGGTTT+TGG - chr1_2:63660557-63660576 Msa0084300:intron 30.0%
ATCTTACAGATTATTCACCT+TGG - chr1_2:63660636-63660655 Msa0084300:intron 30.0%
! TGTATTCTTTTCTTCAACCA+AGG + chr1_2:63660656-63660675 None:intergenic 30.0%
GAATACAAAATCCACGTTAT+TGG - chr1_2:63660669-63660688 Msa0084300:intron 30.0%
ATTCTGCATATAATCCAAAG+GGG + chr1_2:63660753-63660772 None:intergenic 30.0%
CATTCTGCATATAATCCAAA+GGG + chr1_2:63660754-63660773 None:intergenic 30.0%
TCATTCTGCATATAATCCAA+AGG + chr1_2:63660755-63660774 None:intergenic 30.0%
AAGTTATGGTTAATGTAGTC+AGG - chr1_2:63660903-63660922 Msa0084300:intron 30.0%
ATATTGTAATTGCATGCAGT+TGG + chr1_2:63660942-63660961 None:intergenic 30.0%
AACTGCATGCAATTACAATA+TGG - chr1_2:63660941-63660960 Msa0084300:intron 30.0%
TTGAAGACATAAGAAGGAAT+TGG - chr1_2:63661060-63661079 Msa0084300:intron 30.0%
CTCATAGGGATTTATTCAAT+TGG - chr1_2:63661122-63661141 Msa0084300:intron 30.0%
CAATGACATTAAACTACTTG+GGG + chr1_2:63661433-63661452 None:intergenic 30.0%
TATTGATCCCCTTTACAAAA+AGG + chr1_2:63661465-63661484 None:intergenic 30.0%
!!! TGTTATGTGCTTTTTTCAAC+AGG - chr1_2:63661880-63661899 Msa0084300:CDS 30.0%
CTATTACAAGAAGTGGTGTT+TGG + chr1_2:63660424-63660443 None:intergenic 35.0%
CAAACACCACTTCTTGTAAT+AGG - chr1_2:63660422-63660441 Msa0084300:three_prime_UTR 35.0%
GTACTTGTGATATCATTAGC+TGG - chr1_2:63660452-63660471 Msa0084300:three_prime_UTR 35.0%
TACTCACTATAAGATCTACC+AGG + chr1_2:63660614-63660633 None:intergenic 35.0%
GAATGATATGTCTCCAATAC+AGG - chr1_2:63660769-63660788 Msa0084300:intron 35.0%
AAGACTCAGATTGCTACAAA+TGG - chr1_2:63660982-63661001 Msa0084300:intron 35.0%
CACTCATAACATAGCAATCT+TGG + chr1_2:63661017-63661036 None:intergenic 35.0%
!!! ACCATGCTTATTTTGCTCAT+AGG - chr1_2:63661107-63661126 Msa0084300:intron 35.0%
!!! CCATGCTTATTTTGCTCATA+GGG - chr1_2:63661108-63661127 Msa0084300:intron 35.0%
GCTGAAACAGATTATCCATA+TGG - chr1_2:63661173-63661192 Msa0084300:intron 35.0%
TCAATAGTGTTCTCCTCATT+CGG - chr1_2:63661479-63661498 Msa0084300:CDS 35.0%
!!! ACTCACCTACAACCTTTTTT+TGG - chr1_2:63661538-63661557 Msa0084300:intron 35.0%
CTACACCAAAAAAAGGTTGT+AGG + chr1_2:63661546-63661565 None:intergenic 35.0%
!!! CTACAACCTTTTTTTGGTGT+AGG - chr1_2:63661544-63661563 Msa0084300:intron 35.0%
GTTTCACCTACACCAAAAAA+AGG + chr1_2:63661553-63661572 None:intergenic 35.0%
!! TTACCTTTACTTTTGCCACA+TGG - chr1_2:63661771-63661790 Msa0084300:CDS 35.0%
ATTCCATGTGGCAAAAGTAA+AGG + chr1_2:63661777-63661796 None:intergenic 35.0%
!! TTACTTTTGCCACATGGAAT+AGG - chr1_2:63661777-63661796 Msa0084300:CDS 35.0%
! TACTTTTGCCACATGGAATA+GGG - chr1_2:63661778-63661797 Msa0084300:CDS 35.0%
!!! TATGTGCTTTTTTCAACAGG+TGG - chr1_2:63661883-63661902 Msa0084300:CDS 35.0%
ATGATGCTGGAAAGAAACAA+AGG + chr1_2:63662054-63662073 None:intergenic 35.0%
TCCTAGTAGTAGTAGTAGTT+TGG - chr1_2:63662079-63662098 Msa0084300:CDS 35.0%
CTATTACAAGAAGTGGTGTT+TGG + chr1_2:63660424-63660443 None:intergenic 35.0%
CAAACACCACTTCTTGTAAT+AGG - chr1_2:63660422-63660441 Msa0084300:three_prime_UTR 35.0%
GTACTTGTGATATCATTAGC+TGG - chr1_2:63660452-63660471 Msa0084300:three_prime_UTR 35.0%
TACTCACTATAAGATCTACC+AGG + chr1_2:63660614-63660633 None:intergenic 35.0%
GAATGATATGTCTCCAATAC+AGG - chr1_2:63660769-63660788 Msa0084300:intron 35.0%
AAGACTCAGATTGCTACAAA+TGG - chr1_2:63660982-63661001 Msa0084300:intron 35.0%
CACTCATAACATAGCAATCT+TGG + chr1_2:63661017-63661036 None:intergenic 35.0%
!!! ACCATGCTTATTTTGCTCAT+AGG - chr1_2:63661107-63661126 Msa0084300:intron 35.0%
!!! CCATGCTTATTTTGCTCATA+GGG - chr1_2:63661108-63661127 Msa0084300:intron 35.0%
GCTGAAACAGATTATCCATA+TGG - chr1_2:63661173-63661192 Msa0084300:intron 35.0%
TCAATAGTGTTCTCCTCATT+CGG - chr1_2:63661479-63661498 Msa0084300:CDS 35.0%
!!! ACTCACCTACAACCTTTTTT+TGG - chr1_2:63661538-63661557 Msa0084300:intron 35.0%
CTACACCAAAAAAAGGTTGT+AGG + chr1_2:63661546-63661565 None:intergenic 35.0%
!!! CTACAACCTTTTTTTGGTGT+AGG - chr1_2:63661544-63661563 Msa0084300:intron 35.0%
GTTTCACCTACACCAAAAAA+AGG + chr1_2:63661553-63661572 None:intergenic 35.0%
!! TTACCTTTACTTTTGCCACA+TGG - chr1_2:63661771-63661790 Msa0084300:CDS 35.0%
ATTCCATGTGGCAAAAGTAA+AGG + chr1_2:63661777-63661796 None:intergenic 35.0%
!! TTACTTTTGCCACATGGAAT+AGG - chr1_2:63661777-63661796 Msa0084300:CDS 35.0%
! TACTTTTGCCACATGGAATA+GGG - chr1_2:63661778-63661797 Msa0084300:CDS 35.0%
!!! TATGTGCTTTTTTCAACAGG+TGG - chr1_2:63661883-63661902 Msa0084300:CDS 35.0%
ATGATGCTGGAAAGAAACAA+AGG + chr1_2:63662054-63662073 None:intergenic 35.0%
TCCTAGTAGTAGTAGTAGTT+TGG - chr1_2:63662079-63662098 Msa0084300:CDS 35.0%
AGGTTCAGTAACACAGTGAT+AGG - chr1_2:63660305-63660324 Msa0084300:three_prime_UTR 40.0%
GGTTCAGTAACACAGTGATA+GGG - chr1_2:63660306-63660325 Msa0084300:three_prime_UTR 40.0%
TCCATACCAATAATTGGAGC+AGG - chr1_2:63660356-63660375 Msa0084300:three_prime_UTR 40.0%
TCCTGCTCCAATTATTGGTA+TGG + chr1_2:63660360-63660379 None:intergenic 40.0%
CATAGTCCTGCTCCAATTAT+TGG + chr1_2:63660365-63660384 None:intergenic 40.0%
CAATAATTGGAGCAGGACTA+TGG - chr1_2:63660363-63660382 Msa0084300:three_prime_UTR 40.0%
TTCATGTTGCATGTGCACTA+TGG - chr1_2:63660492-63660511 Msa0084300:CDS 40.0%
ATGTGCACTATGGTTGTACT+TGG - chr1_2:63660502-63660521 Msa0084300:CDS 40.0%
!! GGTTTTGGTGTGACTAGTAA+AGG - chr1_2:63660572-63660591 Msa0084300:intron 40.0%
! TTTGGTGTGACTAGTAAAGG+TGG - chr1_2:63660575-63660594 Msa0084300:intron 40.0%
CTTGTGACAAACTTTCCTCT+TGG - chr1_2:63660726-63660745 Msa0084300:intron 40.0%
TTGTAATTGCATGCAGTTGG+TGG + chr1_2:63660939-63660958 None:intergenic 40.0%
GTGTGCTTGAAGACATAAGA+AGG - chr1_2:63661054-63661073 Msa0084300:intron 40.0%
CCCTATGAGCAAAATAAGCA+TGG + chr1_2:63661111-63661130 None:intergenic 40.0%
! GTCATACGGTTTTCACCATA+TGG + chr1_2:63661191-63661210 None:intergenic 40.0%
TGGGTCTAACTTTGGTCATA+CGG + chr1_2:63661205-63661224 None:intergenic 40.0%
TACCAATGGTAATCCCATCT+GGG + chr1_2:63661224-63661243 None:intergenic 40.0%
TTACCAATGGTAATCCCATC+TGG + chr1_2:63661225-63661244 None:intergenic 40.0%
TGAGTCATGAAACGGAAAAG+TGG + chr1_2:63661523-63661542 None:intergenic 40.0%
GTTGTAGGTGAGTCATGAAA+CGG + chr1_2:63661531-63661550 None:intergenic 40.0%
ACATGGAATAGGGATTTGGA+GGG - chr1_2:63661788-63661807 Msa0084300:CDS 40.0%
!!! CTTTTTTCAACAGGTGGAGA+TGG - chr1_2:63661889-63661908 Msa0084300:CDS 40.0%
TATGCTCTATCATCCATGCT+TGG - chr1_2:63661949-63661968 Msa0084300:CDS 40.0%
ATGCTCTATCATCCATGCTT+GGG - chr1_2:63661950-63661969 Msa0084300:CDS 40.0%
GTGAATTGTCACAATCCCTA+TGG + chr1_2:63661984-63662003 None:intergenic 40.0%
TTCACTAGTTCCACAGTGTA+TGG - chr1_2:63661999-63662018 Msa0084300:CDS 40.0%
AGTTCCACAGTGTATGGTAT+TGG - chr1_2:63662005-63662024 Msa0084300:CDS 40.0%
ATGTCCAATACCATACACTG+TGG + chr1_2:63662012-63662031 None:intergenic 40.0%
GCCAAACTACTACTACTACT+AGG + chr1_2:63662083-63662102 None:intergenic 40.0%
AGGTTCAGTAACACAGTGAT+AGG - chr1_2:63660305-63660324 Msa0084300:three_prime_UTR 40.0%
GGTTCAGTAACACAGTGATA+GGG - chr1_2:63660306-63660325 Msa0084300:three_prime_UTR 40.0%
TCCATACCAATAATTGGAGC+AGG - chr1_2:63660356-63660375 Msa0084300:three_prime_UTR 40.0%
TCCTGCTCCAATTATTGGTA+TGG + chr1_2:63660360-63660379 None:intergenic 40.0%
CATAGTCCTGCTCCAATTAT+TGG + chr1_2:63660365-63660384 None:intergenic 40.0%
CAATAATTGGAGCAGGACTA+TGG - chr1_2:63660363-63660382 Msa0084300:three_prime_UTR 40.0%
TTCATGTTGCATGTGCACTA+TGG - chr1_2:63660492-63660511 Msa0084300:CDS 40.0%
ATGTGCACTATGGTTGTACT+TGG - chr1_2:63660502-63660521 Msa0084300:CDS 40.0%
!! GGTTTTGGTGTGACTAGTAA+AGG - chr1_2:63660572-63660591 Msa0084300:intron 40.0%
! TTTGGTGTGACTAGTAAAGG+TGG - chr1_2:63660575-63660594 Msa0084300:intron 40.0%
CTTGTGACAAACTTTCCTCT+TGG - chr1_2:63660726-63660745 Msa0084300:intron 40.0%
TTGTAATTGCATGCAGTTGG+TGG + chr1_2:63660939-63660958 None:intergenic 40.0%
GTGTGCTTGAAGACATAAGA+AGG - chr1_2:63661054-63661073 Msa0084300:intron 40.0%
CCCTATGAGCAAAATAAGCA+TGG + chr1_2:63661111-63661130 None:intergenic 40.0%
! GTCATACGGTTTTCACCATA+TGG + chr1_2:63661191-63661210 None:intergenic 40.0%
TGGGTCTAACTTTGGTCATA+CGG + chr1_2:63661205-63661224 None:intergenic 40.0%
TACCAATGGTAATCCCATCT+GGG + chr1_2:63661224-63661243 None:intergenic 40.0%
TTACCAATGGTAATCCCATC+TGG + chr1_2:63661225-63661244 None:intergenic 40.0%
TGAGTCATGAAACGGAAAAG+TGG + chr1_2:63661523-63661542 None:intergenic 40.0%
GTTGTAGGTGAGTCATGAAA+CGG + chr1_2:63661531-63661550 None:intergenic 40.0%
ACATGGAATAGGGATTTGGA+GGG - chr1_2:63661788-63661807 Msa0084300:CDS 40.0%
!!! CTTTTTTCAACAGGTGGAGA+TGG - chr1_2:63661889-63661908 Msa0084300:CDS 40.0%
TATGCTCTATCATCCATGCT+TGG - chr1_2:63661949-63661968 Msa0084300:CDS 40.0%
ATGCTCTATCATCCATGCTT+GGG - chr1_2:63661950-63661969 Msa0084300:CDS 40.0%
GTGAATTGTCACAATCCCTA+TGG + chr1_2:63661984-63662003 None:intergenic 40.0%
TTCACTAGTTCCACAGTGTA+TGG - chr1_2:63661999-63662018 Msa0084300:CDS 40.0%
AGTTCCACAGTGTATGGTAT+TGG - chr1_2:63662005-63662024 Msa0084300:CDS 40.0%
ATGTCCAATACCATACACTG+TGG + chr1_2:63662012-63662031 None:intergenic 40.0%
GCCAAACTACTACTACTACT+AGG + chr1_2:63662083-63662102 None:intergenic 40.0%
AATTGGAGCAGGACTATGGA+TGG - chr1_2:63660367-63660386 Msa0084300:three_prime_UTR 45.0%
ATAATCCAAAGGGGTCCAAG+AGG + chr1_2:63660744-63660763 None:intergenic 45.0%
GAGTGTGATTCATGCAAAGC+TGG - chr1_2:63661032-63661051 Msa0084300:intron 45.0%
TGACCAAAGTTAGACCCAGA+TGG - chr1_2:63661207-63661226 Msa0084300:CDS 45.0%
GACCAAAGTTAGACCCAGAT+GGG - chr1_2:63661208-63661227 Msa0084300:CDS 45.0%
ATCCCATCTGGGTCTAACTT+TGG + chr1_2:63661213-63661232 None:intergenic 45.0%
TGCCACATGGAATAGGGATT+TGG - chr1_2:63661784-63661803 Msa0084300:CDS 45.0%
CTCCAAATCCCTATTCCATG+TGG + chr1_2:63661789-63661808 None:intergenic 45.0%
CACATGGAATAGGGATTTGG+AGG - chr1_2:63661787-63661806 Msa0084300:CDS 45.0%
GAGATGGTTGCATGATAGGA+AGG - chr1_2:63661905-63661924 Msa0084300:CDS 45.0%
TCTATCATCCATGCTTGGGT+GGG - chr1_2:63661954-63661973 Msa0084300:CDS 45.0%
! TACTAGGAAGCTGATGATGC+TGG + chr1_2:63662067-63662086 None:intergenic 45.0%
AATTGGAGCAGGACTATGGA+TGG - chr1_2:63660367-63660386 Msa0084300:three_prime_UTR 45.0%
ATAATCCAAAGGGGTCCAAG+AGG + chr1_2:63660744-63660763 None:intergenic 45.0%
GAGTGTGATTCATGCAAAGC+TGG - chr1_2:63661032-63661051 Msa0084300:intron 45.0%
TGACCAAAGTTAGACCCAGA+TGG - chr1_2:63661207-63661226 Msa0084300:CDS 45.0%
GACCAAAGTTAGACCCAGAT+GGG - chr1_2:63661208-63661227 Msa0084300:CDS 45.0%
ATCCCATCTGGGTCTAACTT+TGG + chr1_2:63661213-63661232 None:intergenic 45.0%
TGCCACATGGAATAGGGATT+TGG - chr1_2:63661784-63661803 Msa0084300:CDS 45.0%
CTCCAAATCCCTATTCCATG+TGG + chr1_2:63661789-63661808 None:intergenic 45.0%
CACATGGAATAGGGATTTGG+AGG - chr1_2:63661787-63661806 Msa0084300:CDS 45.0%
GAGATGGTTGCATGATAGGA+AGG - chr1_2:63661905-63661924 Msa0084300:CDS 45.0%
TCTATCATCCATGCTTGGGT+GGG - chr1_2:63661954-63661973 Msa0084300:CDS 45.0%
! TACTAGGAAGCTGATGATGC+TGG + chr1_2:63662067-63662086 None:intergenic 45.0%
!! ATTAAAAAAATAAACTTATT+AGG - chr1_2:63661650-63661669 Msa0084300:CDS 5.0%
!! ATTAAAAAAATAAACTTATT+AGG - chr1_2:63661650-63661669 Msa0084300:CDS 5.0%
GGTGTGACTAGTAAAGGTGG+TGG - chr1_2:63660578-63660597 Msa0084300:intron 50.0%
ACTTTCCTCTTGGACCCCTT+TGG - chr1_2:63660736-63660755 Msa0084300:intron 50.0%
GACCCAGATGGGATTACCAT+TGG - chr1_2:63661219-63661238 Msa0084300:CDS 50.0%
GGTGGAGATGGTTGCATGAT+AGG - chr1_2:63661901-63661920 Msa0084300:CDS 50.0%
CTCTATCATCCATGCTTGGG+TGG - chr1_2:63661953-63661972 Msa0084300:CDS 50.0%
GGTGTGACTAGTAAAGGTGG+TGG - chr1_2:63660578-63660597 Msa0084300:intron 50.0%
ACTTTCCTCTTGGACCCCTT+TGG - chr1_2:63660736-63660755 Msa0084300:intron 50.0%
GACCCAGATGGGATTACCAT+TGG - chr1_2:63661219-63661238 Msa0084300:CDS 50.0%
GGTGGAGATGGTTGCATGAT+AGG - chr1_2:63661901-63661920 Msa0084300:CDS 50.0%
CTCTATCATCCATGCTTGGG+TGG - chr1_2:63661953-63661972 Msa0084300:CDS 50.0%
GAGCAGGACTATGGATGGCA+AGG - chr1_2:63660372-63660391 Msa0084300:three_prime_UTR 55.0%
GAGCAGGACTATGGATGGCA+AGG - chr1_2:63660372-63660391 Msa0084300:three_prime_UTR 55.0%
!! GGTGGTGGTGTAGAAGTGCC+TGG - chr1_2:63660593-63660612 Msa0084300:intron 60.0%
! ATGGAGCACCCACCCAAGCA+TGG + chr1_2:63661965-63661984 None:intergenic 60.0%
TGCTTGGGTGGGTGCTCCAT+AGG - chr1_2:63661965-63661984 Msa0084300:CDS 60.0%
GCTTGGGTGGGTGCTCCATA+GGG - chr1_2:63661966-63661985 Msa0084300:CDS 60.0%
!! GGTGGTGGTGTAGAAGTGCC+TGG - chr1_2:63660593-63660612 Msa0084300:intron 60.0%
! ATGGAGCACCCACCCAAGCA+TGG + chr1_2:63661965-63661984 None:intergenic 60.0%
TGCTTGGGTGGGTGCTCCAT+AGG - chr1_2:63661965-63661984 Msa0084300:CDS 60.0%
GCTTGGGTGGGTGCTCCATA+GGG - chr1_2:63661966-63661985 Msa0084300:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 63660254 63662176 63660254 ID=Msa0084300;Name=Msa0084300
chr1_2 mRNA 63660254 63662176 63660254 ID=Msa0084300-mRNA-1;Parent=Msa0084300;Name=Msa0084300-mRNA-1;_AED=0.03;_eAED=0.03;_QI=45|1|1|1|1|1|3|235|283
chr1_2 exon 63660254 63660528 63660254 ID=Msa0084300-mRNA-1:exon:21350;Parent=Msa0084300-mRNA-1
chr1_2 exon 63661190 63661509 63661190 ID=Msa0084300-mRNA-1:exon:21349;Parent=Msa0084300-mRNA-1
chr1_2 exon 63661640 63662176 63661640 ID=Msa0084300-mRNA-1:exon:21348;Parent=Msa0084300-mRNA-1
chr1_2 five_prime_UTR 63662132 63662176 63662132 ID=Msa0084300-mRNA-1:five_prime_utr;Parent=Msa0084300-mRNA-1
chr1_2 CDS 63661640 63662131 63661640 ID=Msa0084300-mRNA-1:cds;Parent=Msa0084300-mRNA-1
chr1_2 CDS 63661190 63661509 63661190 ID=Msa0084300-mRNA-1:cds;Parent=Msa0084300-mRNA-1
chr1_2 CDS 63660489 63660528 63660489 ID=Msa0084300-mRNA-1:cds;Parent=Msa0084300-mRNA-1
chr1_2 three_prime_UTR 63660254 63660488 63660254 ID=Msa0084300-mRNA-1:three_prime_utr;Parent=Msa0084300-mRNA-1
Gene Sequence

>Msa0084300

ATGTATAGGTTCAGTAACACAGTGATAGGGATCTTAAACTTGCTAACACTTTTAGCATCCATACCAATAATTGGAGCAGGACTATGGATGGCAAGGAGCAGCACAACTTGTGCAAATTTTCTCCAAACACCACTTCTTGTAATAGGTTTTATTGTACTTGTGATATCATTAGCTGGTTTCATTGGTGCTTGTTTTCATGTTGCATGTGCACTATGGTTGTACTTGGTGATTATGCTGTTACTAATAGTAGCACTATTGGGTTTGACTATATTTGGTTTTGGTGTGACTAGTAAAGGTGGTGGTGTAGAAGTGCCTGGTAGATCTTATAGTGAGTATCATCTTACAGATTATTCACCTTGGTTGAAGAAAAGAATACAAAATCCACGTTATTGGAATACTATAAAGAATTGTATTTTAGGGTCTAAAACTTGTGACAAACTTTCCTCTTGGACCCCTTTGGATTATATGCAGAATGATATGTCTCCAATACAGTCAGGATGTTGTAAGCCACCAACTGCATGCAATTACAATATGGAAGCAGTGATGATGACACAAGACTCAGATTGCTACAAATGGAGCAATGAACCAAGATTGCTATGTTATGAGTGTGATTCATGCAAAGCTGGTGTGCTTGAAGACATAAGAAGGAATTGGCATAAGCTATCAGTTCTCACTGTCACCATGCTTATTTTGCTCATAGGGATTTATTCAATTGGATGTTGTGCTTTCAGAAATGCAAGAAGAGCTGAAACAGATTATCCATATGGTGAAAACCGTATGACCAAAGTTAGACCCAGATGGGATTACCATTGGTGGAGATGGTTGCATGATAGGAAGGAACAGCTTTATTAG
Protein sequence

>Msa0084300

MYRFSNTVIGILNLLTLLASIPIIGAGLWMARSSTTCANFLQTPLLVIGFIVLVISLAGFIGACFHVACALWLYLVIMLLLIVALLGLTIFGFGVTSKGGGVEVPGRSYSEYHLTDYSPWLKKRIQNPRYWNTIKNCILGSKTCDKLSSWTPLDYMQNDMSPIQSGCCKPPTACNYNMEAVMMTQDSDCYKWSNEPRLLCYECDSCKAGVLEDIRRNWHKLSVLTVTMLILLIGIYSIGCCAFRNARRAETDYPYGENRMTKVRPRWDYHWWRWLHDRKEQLY*