Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0192870 | XP_003593732.1 | 98.750 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 6.80e-46 | 151 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0192870 | sp|O82275|TAX1_ARATH | 75.000 | 76 | 17 | 1 | 5 | 80 | 2 | 75 | 3.41e-26 | 94.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0192870 | G7IKA1 | 98.750 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 3.25e-46 | 151 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0047660 | Msa0192870 | -0.806049 | 9.934222e-50 | -8.615850e-47 |
| Msa0127220 | Msa0192870 | -0.801025 | 1.093601e-48 | -8.615850e-47 |
| Msa0192870 | Msa0235000 | 0.808413 | 3.135901e-50 | -8.615850e-47 |
| Msa0192870 | Msa0273400 | 0.861764 | 8.157720e-64 | -8.615850e-47 |
| Msa0192870 | Msa0287480 | 0.804361 | 2.241796e-49 | -8.615850e-47 |
| Msa0192870 | Msa0313660 | 0.843565 | 1.296248e-58 | -8.615850e-47 |
| Msa0192870 | Msa0331580 | 0.823444 | 1.380696e-53 | -8.615850e-47 |
| Msa0192870 | Msa0375790 | 0.804413 | 2.185763e-49 | -8.615850e-47 |
| Msa0192870 | Msa0449500 | 0.814664 | 1.372711e-51 | -8.615850e-47 |
| Msa0192870 | Msa0524670 | 0.810389 | 1.180816e-50 | -8.615850e-47 |
| Msa0192870 | Msa0529110 | 0.814344 | 1.616385e-51 | -8.615850e-47 |
| Msa0192870 | Msa0582550 | -0.804857 | 1.765886e-49 | -8.615850e-47 |
| Msa0192870 | Msa0590620 | -0.807165 | 5.776235e-50 | -8.615850e-47 |
| Msa0192870 | Msa0595270 | 0.807565 | 4.751578e-50 | -8.615850e-47 |
| Msa0192870 | Msa0714230 | -0.810697 | 1.013363e-50 | -8.615850e-47 |
| Msa0192870 | Msa0773030 | 0.800673 | 1.290901e-48 | -8.615850e-47 |
| Msa0192870 | Msa0873910 | 0.819689 | 1.017904e-52 | -8.615850e-47 |
| Msa0192870 | Msa0906660 | 0.800881 | 1.170671e-48 | -8.615850e-47 |
| Msa0192870 | Msa0937160 | -0.806530 | 7.866333e-50 | -8.615850e-47 |
| Msa0192870 | Msa1034690 | 0.819551 | 1.094708e-52 | -8.615850e-47 |
| Msa0192870 | Msa1062060 | 0.805053 | 1.606808e-49 | -8.615850e-47 |
| Msa0192870 | Msa1089990 | 0.824443 | 8.049742e-54 | -8.615850e-47 |
| Msa0192870 | Msa1121720 | -0.800532 | 1.378732e-48 | -8.615850e-47 |
| Msa0192870 | Msa1221270 | 0.809134 | 2.198214e-50 | -8.615850e-47 |
| Msa0192870 | Msa1264300 | -0.807796 | 4.242634e-50 | -8.615850e-47 |
| Msa0192870 | Msa1269200 | 0.803391 | 3.565224e-49 | -8.615850e-47 |
| Msa0192870 | Msa1334350 | -0.812841 | 3.459804e-51 | -8.615850e-47 |
| Msa0192870 | Msa1356100 | -0.800373 | 1.485634e-48 | -8.615850e-47 |
| Msa0192870 | Msa1357710 | -0.803106 | 4.084546e-49 | -8.615850e-47 |
| Msa0192870 | Msa1402340 | -0.812531 | 4.046068e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0192870 | MtrunA17_Chr2g0283451 | 98.750 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 6.26e-50 | 151 |
| Msa0192870 | MtrunA17_Chr2g0325441 | 73.684 | 76 | 20 | 0 | 5 | 80 | 2 | 77 | 4.07e-35 | 114 |
| Msa0192870 | MtrunA17_Chr4g0040991 | 75.000 | 76 | 19 | 0 | 5 | 80 | 2 | 77 | 5.92e-35 | 113 |
| Msa0192870 | MtrunA17_Chr2g0324911 | 59.459 | 74 | 30 | 0 | 7 | 80 | 5 | 78 | 9.40e-21 | 77.8 |
| Msa0192870 | MtrunA17_Chr4g0040491 | 56.944 | 72 | 31 | 0 | 9 | 80 | 8 | 79 | 1.61e-14 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0192870 | AT2G31090.1 | 75.000 | 76 | 17 | 1 | 5 | 80 | 2 | 75 | 3.46e-27 | 94.4 |
| Msa0192870 | AT2G20562.1 | 60.870 | 69 | 27 | 0 | 12 | 80 | 5 | 73 | 5.39e-20 | 76.3 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTTGTAGGTGCAGTTATT+TGG | 0.227999 | 2_1:+8667913 | Msa0192870:CDS |
| AACCACTCCATAACCTTAAT+TGG | 0.239487 | 2_1:-8668137 | None:intergenic |
| TCTCTCATAATCTCCTTTGT+AGG | 0.287883 | 2_1:+8667900 | Msa0192870:CDS |
| CACATGGTTTAATTACCTAA+TGG | 0.296204 | 2_1:-8667807 | None:intergenic |
| TCTCATCAAGGCTCCAATTA+AGG | 0.314893 | 2_1:+8668124 | Msa0192870:CDS |
| ACATGGTTTAATTACCTAAT+GGG | 0.328884 | 2_1:-8667806 | None:intergenic |
| TTTAAGAACTTCAACCCATT+AGG | 0.359993 | 2_1:+8667792 | None:intergenic |
| CAAGGCTCCAATTAAGGTTA+TGG | 0.360751 | 2_1:+8668130 | Msa0192870:CDS |
| GGTGCAGTTATTTGGATTGT+TGG | 0.388294 | 2_1:+8667921 | Msa0192870:CDS |
| GTGCAGTTATTTGGATTGTT+GGG | 0.397665 | 2_1:+8667922 | Msa0192870:CDS |
| GTTTGCTCTGGCTCTCATCA+AGG | 0.420977 | 2_1:+8668112 | Msa0192870:CDS |
| CCTCATAGTTGAGTTTGCTC+TGG | 0.438328 | 2_1:+8668100 | Msa0192870:CDS |
| TCTCTTCTAACATGGAATCT+TGG | 0.440539 | 2_1:-8668165 | None:intergenic |
| GATTGTAAATTCAGGCCTCT+TGG | 0.461509 | 2_1:+8667846 | Msa0192870:CDS |
| ATGAGAGACAAGAAAGCAAA+AGG | 0.478563 | 2_1:-8667885 | None:intergenic |
| AGAAAGCAAAAGGAAGGCCT+AGG | 0.499755 | 2_1:-8667875 | None:intergenic |
| CCAGAGCAAACTCAACTATG+AGG | 0.515083 | 2_1:-8668100 | None:intergenic |
| GTGGTGAGGATTGTAAATTC+AGG | 0.531054 | 2_1:+8667838 | Msa0192870:CDS |
| TAAACCATGTGTTGCTGTTG+TGG | 0.534246 | 2_1:+8667819 | Msa0192870:exon |
| CCAAATAACTGCACCTACAA+AGG | 0.535186 | 2_1:-8667913 | None:intergenic |
| TACAAATATCTCTTCTAACA+TGG | 0.565698 | 2_1:-8668173 | None:intergenic |
| GAGACAAGAAAGCAAAAGGA+AGG | 0.614663 | 2_1:-8667881 | None:intergenic |
| AGGGTCACGCAAAGACAGCA+TGG | 0.623057 | 2_1:-8668080 | None:intergenic |
| CAGAGCAAACTCAACTATGA+GGG | 0.623831 | 2_1:-8668099 | None:intergenic |
| CTCACCACAACAGCAACACA+TGG | 0.625747 | 2_1:-8667823 | None:intergenic |
| CATGTGTTGCTGTTGTGGTG+AGG | 0.646232 | 2_1:+8667824 | Msa0192870:exon |
| CTCCAATTAAGGTTATGGAG+TGG | 0.661349 | 2_1:+8668135 | Msa0192870:CDS |
| AGGCCTAGGAGAAAACCAAG+AGG | 0.747286 | 2_1:-8667861 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAAAATACTAATAGAT+GGG | - | chr2_1:8667964-8667983 | None:intergenic | 15.0% |
| !! | CAAAAGAAAATACTAATAGA+TGG | - | chr2_1:8667965-8667984 | None:intergenic | 20.0% |
| ! | ACATGGTTTAATTACCTAAT+GGG | - | chr2_1:8667809-8667828 | None:intergenic | 25.0% |
| CACATGGTTTAATTACCTAA+TGG | - | chr2_1:8667810-8667829 | None:intergenic | 30.0% | |
| GAACTAGTTATGAATGTGTA+TGG | + | chr2_1:8668020-8668039 | Msa0192870:intron | 30.0% | |
| !! | AATGTGTATGGTGCTATATA+TGG | + | chr2_1:8668032-8668051 | Msa0192870:intron | 30.0% |
| ATGAGAGACAAGAAAGCAAA+AGG | - | chr2_1:8667888-8667907 | None:intergenic | 35.0% | |
| TCTCTCATAATCTCCTTTGT+AGG | + | chr2_1:8667900-8667919 | Msa0192870:CDS | 35.0% | |
| GTGCAGTTATTTGGATTGTT+GGG | + | chr2_1:8667922-8667941 | Msa0192870:CDS | 35.0% | |
| CTAATAGATGGGAAAAGAGA+GGG | - | chr2_1:8667953-8667972 | None:intergenic | 35.0% | |
| ACTAATAGATGGGAAAAGAG+AGG | - | chr2_1:8667954-8667973 | None:intergenic | 35.0% | |
| AACCACTCCATAACCTTAAT+TGG | - | chr2_1:8668140-8668159 | None:intergenic | 35.0% | |
| ! | TAAACCATGTGTTGCTGTTG+TGG | + | chr2_1:8667819-8667838 | Msa0192870:exon | 40.0% |
| GTGGTGAGGATTGTAAATTC+AGG | + | chr2_1:8667838-8667857 | Msa0192870:CDS | 40.0% | |
| GATTGTAAATTCAGGCCTCT+TGG | + | chr2_1:8667846-8667865 | Msa0192870:CDS | 40.0% | |
| GAGACAAGAAAGCAAAAGGA+AGG | - | chr2_1:8667884-8667903 | None:intergenic | 40.0% | |
| CCAAATAACTGCACCTACAA+AGG | - | chr2_1:8667916-8667935 | None:intergenic | 40.0% | |
| CCTTTGTAGGTGCAGTTATT+TGG | + | chr2_1:8667913-8667932 | Msa0192870:CDS | 40.0% | |
| GGTGCAGTTATTTGGATTGT+TGG | + | chr2_1:8667921-8667940 | Msa0192870:CDS | 40.0% | |
| !! | TGTATGGTGCTATATATGGC+AGG | + | chr2_1:8668036-8668055 | Msa0192870:intron | 40.0% |
| CAGAGCAAACTCAACTATGA+GGG | - | chr2_1:8668102-8668121 | None:intergenic | 40.0% | |
| TCTCATCAAGGCTCCAATTA+AGG | + | chr2_1:8668124-8668143 | Msa0192870:CDS | 40.0% | |
| CAAGGCTCCAATTAAGGTTA+TGG | + | chr2_1:8668130-8668149 | Msa0192870:CDS | 40.0% | |
| CTCCAATTAAGGTTATGGAG+TGG | + | chr2_1:8668135-8668154 | Msa0192870:CDS | 40.0% | |
| AGAAAGCAAAAGGAAGGCCT+AGG | - | chr2_1:8667878-8667897 | None:intergenic | 45.0% | |
| CCAGAGCAAACTCAACTATG+AGG | - | chr2_1:8668103-8668122 | None:intergenic | 45.0% | |
| ! | CCTCATAGTTGAGTTTGCTC+TGG | + | chr2_1:8668100-8668119 | Msa0192870:CDS | 45.0% |
| CTCACCACAACAGCAACACA+TGG | - | chr2_1:8667826-8667845 | None:intergenic | 50.0% | |
| ! | CATGTGTTGCTGTTGTGGTG+AGG | + | chr2_1:8667824-8667843 | Msa0192870:exon | 50.0% |
| !! | AGGCCTCTTGGTTTTCTCCT+AGG | + | chr2_1:8667858-8667877 | Msa0192870:CDS | 50.0% |
| AGGCCTAGGAGAAAACCAAG+AGG | - | chr2_1:8667864-8667883 | None:intergenic | 50.0% | |
| !! | GTTTGCTCTGGCTCTCATCA+AGG | + | chr2_1:8668112-8668131 | Msa0192870:CDS | 50.0% |
| AGGGTCACGCAAAGACAGCA+TGG | - | chr2_1:8668083-8668102 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 8667795 | 8668181 | 8667795 | ID=Msa0192870;Name=Msa0192870 |
| chr2_1 | mRNA | 8667795 | 8668181 | 8667795 | ID=Msa0192870-mRNA-1;Parent=Msa0192870;Name=Msa0192870-mRNA-1;_AED=0.15;_eAED=0.15;_QI=30|1|0.5|1|1|1|2|0|80 |
| chr2_1 | exon | 8667795 | 8667943 | 8667795 | ID=Msa0192870-mRNA-1:exon:3985;Parent=Msa0192870-mRNA-1 |
| chr2_1 | exon | 8668058 | 8668181 | 8668058 | ID=Msa0192870-mRNA-1:exon:3986;Parent=Msa0192870-mRNA-1 |
| chr2_1 | five_prime_UTR | 8667795 | 8667824 | 8667795 | ID=Msa0192870-mRNA-1:five_prime_utr;Parent=Msa0192870-mRNA-1 |
| chr2_1 | CDS | 8667825 | 8667943 | 8667825 | ID=Msa0192870-mRNA-1:cds;Parent=Msa0192870-mRNA-1 |
| chr2_1 | CDS | 8668058 | 8668181 | 8668058 | ID=Msa0192870-mRNA-1:cds;Parent=Msa0192870-mRNA-1 |
| Gene Sequence |
| Protein sequence |
